P38892 (CRG1_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Probable S-adenosylmethionine-dependent methyltransferase CRG1
EC=2.1.1.-

Alternative name(s):
Cantharidin resistance protein 1

Gene names

Name:	CRG1
Ordered Locus Names:	YHR209W

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › Saccharomyces cerevisiae

Protein attributes

Sequence length	291 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Probable S-adenosylmethionine-dependent methyltransferase which mediates cantharidin resistance. Ref.4 Ref.8
Subcellular location	Cytoplasm Ref.5.
Induction	By cell wall perturbation. Ref.7
Miscellaneous	Present with 4380 molecules/cell in log phase SD medium.
Sequence similarities	Belongs to the methyltransferase superfamily.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	lipid homeostasis Inferred from mutant phenotype PubMed 22028670. Source: SGD
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	S-adenosylmethionine-dependent methyltransferase activity Inferred from direct assay PubMed 22028670. Source: SGD mRNA binding Inferred from direct assay PubMed 20844764. Source: SGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 291	291	Probable S-adenosylmethionine-dependent methyltransferase CRG1		PRO_0000202943
Experimental info
	Sequence conflict	23	1	Y → C in AAS56093. Ref.3

Sequences

	Sequence		Length	Mass (Da)
	P38892 [UniParc]. Last modified February 1, 1995. Version 1. Checksum: C1DFE5F73BAAC617	FASTA	291	33,785
	10 20 30 40 50 60 MPKTSYLNKN FESAHYNNVR PSYPLSLVNE IMKFHKGTRK SLVDIGCGTG KATFVVEPYF 70 80 90 100 110 120 KEVIGIDPSS AMLSIAEKET NERRLDKKIR FINAPGEDLS SIRPESVDMV ISAEAIHWCN 130 140 150 160 170 180 LERLFQQVSS ILRSDGTFAF WFYIQPEFVD FPEALNVYYK YGWSKDYMGK YLNDNQREIL 190 200 210 220 230 240 LNYGGEKLRS LLSDRFGDIE VTIYSPSDPN ASTVTAENSQ FLWRAAITLN QFKEFVKSWS 250 260 270 280 290 IYTSWARDNP SKPDIADIFI NELKEICHCE DLNVPLKIEW STFYYLCRKR E

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Complete nucleotide sequence of Saccharomyces cerevisiae chromosome VIII." Johnston M., Andrews S., Brinkman R., Cooper J., Ding H., Dover J., Du Z., Favello A., Fulton L., Gattung S., Geisel C., Kirsten J., Kucaba T., Hillier L.W., Jier M., Johnston L., Langston Y., Latreille P. , Louis E.J., Macri C., Mardis E., Menezes S., Mouser L., Nhan M., Rifkin L., Riles L., St Peter H., Trevaskis E., Vaughan K., Vignati D., Wilcox L., Wohldman P., Waterston R., Wilson R., Vaudin M. Science 265:2077-2082(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[2]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[3]	"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae." Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J. Genome Res. 17:536-543(2007) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[4]	"S-adenosylmethionine-dependent methylation in Saccharomyces cerevisiae. Identification of a novel protein arginine methyltransferase." Niewmierzycka A., Clarke S. J. Biol. Chem. 274:814-824(1999) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
[5]	"Global analysis of protein localization in budding yeast." Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K. Nature 425:686-691(2003) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[6]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[7]	"Characterization of the transcriptional response to cell wall stress in Saccharomyces cerevisiae." Boorsma A., de Nobel H., ter Riet B., Bargmann B., Brul S., Hellingwerf K.J., Klis F.M. Yeast 21:413-427(2004) [PubMed] [Europe PMC] [Abstract] Cited for: INDUCTION.
[8]	"An integrated platform of genomic assays reveals small-molecule bioactivities." Hoon S., Smith A.M., Wallace I.M., Suresh S., Miranda M., Fung E., Proctor M., Shokat K.M., Zhang C., Davis R.W., Giaever G., St Onge R.P., Nislow C. Nat. Chem. Biol. 4:498-506(2008) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U00029 Genomic DNA. Translation: AAB69732.1. AY557767 Genomic DNA. Translation: AAS56093.1. BK006934 Genomic DNA. Translation: DAA06902.1.
PIR	S48990.
RefSeq	NP_012079.1. NM_001179340.1.
3D structure databases
ProteinModelPortal	P38892.
SMR	P38892. Positions 31-289.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-828N.
MINT	MINT-488540.
STRING	4932.YHR209W.
Proteomic databases
PaxDb	P38892.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YHR209W; YHR209W; YHR209W.
GeneID	856616.
KEGG	sce:YHR209W.
Organism-specific databases
CYGD	YHR209w.
SGD	S000001252. CRG1.
Phylogenomic databases
eggNOG	COG0500.
GeneTree	ENSGT00510000049434.
HOGENOM	HOG000246573.
OMA	VEVETEW.
OrthoDB	EOG4897WX.
Enzyme and pathway databases
BioCyc	YEAST:G3O-31234-MONOMER.
Gene expression databases
Genevestigator	P38892.
GermOnline	YHR209W. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR013216. Methyltransf_11. [Graphical view]
Pfam	PF08241. Methyltransf_11. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	982544.

Entry information

Entry name

CRG1_YEAST

Accession

Primary (citable) accession number: P38892
Secondary accession number(s): D3DLF8, Q6Q5R3

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1995
Last sequence update:	February 1, 1995
Last modified:	May 29, 2013
This is version 81 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program