Transcription factor SKN7 - Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

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P38889 (SKN7_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 120. History...

Clusters with 100%, 90%, 50% identity |

Documents (3) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Transcription factor SKN7

Alternative name(s):
Peroxide sensitivity protein 9

Gene names

Name:	SKN7
Synonyms:	BRY1, POS9
Ordered Locus Names:	YHR206W

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces ›

Protein attributes

Sequence length	622 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Transcription factor that is part of a SLN1-YPD1-SKN7 two-component regulatory system, which controls gene expression in response to changes in the osmolarity of the extracellular environment. Under low osmotic conditions, phosphorylated and activated by the phosphorelay intermediate protein YPD1. Also activated in response to oxidative stress, independent on the two-component regulatory system. Regulates heat shock genes in response to oxidative stress and genes involved in cell wall integrity in response to osmotic changes. Ref.2 Ref.6 Ref.7 Ref.8
Subcellular location	Nucleus Ref.8 Ref.9.
Post-translational modification	The phosphorelay mechanism involves the sequential transfer of a phosphate group from 'His-576' (H1) to 'Asp-1144' (D1) of SLN1, then to 'His-64' (H2) of YPD1 and finally to Asp-427 (D2) of SKN7.
Miscellaneous	Present with 2570 molecules/cell in log phase SD medium.
Sequence similarities	Contains 1 response regulatory domain.

Ontologies

Keywords
Biological process	Transcription Transcription regulation Two-component regulatory system
Cellular component	Nucleus
Ligand	DNA-binding
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	regulation of cell size Inferred from mutant phenotype PubMed 17302939. Source: SGD regulation of transcription from RNA polymerase II promoter in response to oxidative stress Inferred from mutant phenotype PubMed 17965252. Source: SGD response to osmotic stress Traceable author statement PubMed 11854400. Source: SGD response to singlet oxygen Inferred from mutant phenotype PubMed 16862604. Source: SGD transcription, DNA-dependent Inferred from direct assay PubMed 11854400. Source: SGD
Cellular_component	nucleus Inferred from direct assay Ref.8. Source: SGD
Molecular_function	phosphorelay response regulator activity Inferred from direct assay PubMed 11073911. Source: SGD sequence-specific DNA binding Inferred from direct assay PubMed 19111667 PubMed 19158363. Source: SGD sequence-specific DNA binding transcription factor activity Inferred from direct assay PubMed 11854400. Source: SGD
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 622

622

Transcription factor SKN7

PRO_0000081404

Regions

Domain

378 – 492

115

Response regulatory

DNA binding

86 – 190

105

By similarity

Amino acid modifications

Modified residue

Phosphothreonine Ref.11

Modified residue

Phosphoserine Ref.11

Modified residue

Phosphoserine Ref.11

Modified residue

427

4-aspartylphosphate

Experimental info

Mutagenesis

427

D → A: No activity. Ref.2 Ref.6

Mutagenesis

427

D → E: Augments activity. Ref.2 Ref.6

Mutagenesis

427

D → N: Diminishes activity. Ref.2 Ref.6

Mutagenesis

427

D → R: No activity. Ref.2 Ref.6

Sequence conflict

T → A in AAU09745. Ref.5

Sequences

Sequence

Length

Mass (Da)

Tools

P38889 [UniParc].

Last modified February 1, 1995. Version 1.
Checksum: 4C732FD66E326742
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FASTA

622

69,202

        10         20         30         40         50         60 
MSFSTINSNV NKTTGDSNNN TTENSSTADL LGMDLLQSGP RLMNTMQPNN SSDMLHINNK 

        70         80         90        100        110        120 
TNNVQQPAGN TNISSANAGA KAPANEFVRK LFRILENNEY PDIVTWTENG KSFVVLDTGK 

       130        140        150        160        170        180 
FTTHILPNHF KHSNFASFVR QLNKYDFHKV KRSPEERQRC KYGEQSWEFQ HPEFRVHYGK 

       190        200        210        220        230        240 
GLDNIKRKIP AQRKVLLDES QKALLHFNSE GTNPNNPSGS LLNESTTELL LSNTVSKDAF 

       250        260        270        280        290        300 
GNLRRRVDKL QKELDMSKME SYATKVELQK LNSKYNTVIE SLITFKTINE NLLNNFNTLC 

       310        320        330        340        350        360 
STLANNGIEV PIFGDNGNRN PTGNTNPATT TAIQSNNNTN NASPATSTVS LQLPNLPDQN 

       370        380        390        400        410        420 
SLTPNAQNNT VTLRKGFHVL LVEDDAVSIQ LCSKFLRKYG CTVQVVSDGL SAISTLEKYR 

       430        440        450        460        470        480 
YDLVLMDIVM PNLDGATATS IVRSFDNETP IIAMTGNIMN QDLITYLQHG MNDILAKPFT 

       490        500        510        520        530        540 
RDDLHSILIR YLKDRIPLCE QQLPPRNSSP QTHSNTNTAN SNPNTINEQS LAMLPQDNPS 

       550        560        570        580        590        600 
TTTPVTPGAS ISSAQHVQQG QQEQQHQIFH AQQQQQHHNA IANARSDVAI PNLEHEINTV 

       610        620 
PHSSMGSTPQ LPQSTLQENQ LS

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"SKN7, a yeast multicopy suppressor of a mutation affecting cell wall beta-glucan assembly, encodes a product with domains homologous to prokaryotic two-component regulators and to heat shock transcription factors." Brown J.L., North S., Bussey H. J. Bacteriol. 175:6908-6915(1993) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE.
[2]	"The response regulator-like protein Pos9/Skn7 of Saccharomyces cerevisiae is involved in oxidative stress resistance." Krems B., Charizanis C., Entian K.-D. Curr. Genet. 29:327-334(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, MUTAGENESIS OF ASP-427.
[3]	"Complete nucleotide sequence of Saccharomyces cerevisiae chromosome VIII." Johnston M., Andrews S., Brinkman R., Cooper J., Ding H., Dover J., Du Z., Favello A., Fulton L., Gattung S., Geisel C., Kirsten J., Kucaba T., Hillier L.W., Jier M., Johnston L., Langston Y., Latreille P. Vaudin M. Science 265:2077-2082(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[4]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[5]	"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae." Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. LaBaer J. Genome Res. 17:536-543(2007) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[6]	"Yeast Skn7p functions in a eukaryotic two-component regulatory pathway." Brown J.L., Bussey H., Stewart R.C. EMBO J. 13:5186-5194(1994) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, MUTAGENESIS OF ASP-427.
[7]	"The yeast histidine protein kinase, Sln1p, mediates phosphotransfer to two response regulators, Ssk1p and Skn7p." Li S., Ault A., Malone C.L., Raitt D., Dean S., Johnston L.H., Deschenes R.J., Fassler J.S. EMBO J. 17:6952-6962(1998) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, PHOSPHORYLATION AT ASP-427.
[8]	"The Skn7 response regulator of Saccharomyces cerevisiae interacts with Hsf1 in vivo and is required for the induction of heat shock genes by oxidative stress." Raitt D.C., Johnson A.L., Erkine A.M., Makino K., Morgan B., Gross D.S., Johnston L.H. Mol. Biol. Cell 11:2335-2347(2000) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION.
[9]	"Saccharomyces cerevisiae histidine phosphotransferase Ypd1p shuttles between the nucleus and cytoplasm for SLN1-dependent phosphorylation of Ssk1p and Skn7p." Lu J.M.-Y., Deschenes R.J., Fassler J.S. Eukaryot. Cell 2:1304-1314(2003) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION.
[10]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[11]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-27; SER-51 AND SER-52, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U00485 Unassigned DNA. Translation: AAC48911.1. X83031 Genomic DNA. Translation: CAA58143.1. U00029 Genomic DNA. Translation: AAB69734.1. AY723828 Genomic DNA. Translation: AAU09745.1. BK006934 Genomic DNA. Translation: DAA06899.1.
PIR	A49344.
RefSeq	NP_012076.3. NM_001179337.3.
3D structure databases
ProteinModelPortal	P38889.
SMR	P38889. Positions 87-171, 379-535.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-2403N.
IntAct	P38889. 8 interactions.
Proteomic databases
PaxDb	P38889.
PeptideAtlas	P38889.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YHR206W; YHR206W; YHR206W.
GeneID	856613.
KEGG	sce:YHR206W. sce:YHR208W.
Organism-specific databases
CYGD	YHR206w.
SGD	S000001249. SKN7.
Phylogenomic databases
eggNOG	COG0784.
GeneTree	ENSGT00610000087034.
HOGENOM	HOG000248776.
KO	K00826. K15859.
OMA	QHGMNDI.
OrthoDB	EOG4TMV98.
Gene expression databases
Genevestigator	P38889.
GermOnline	YHR206W. Saccharomyces cerevisiae.
Family and domain databases
Gene3D	1.10.10.10. 1 hit.
InterPro	IPR011006. CheY-like_superfamily. IPR000232. HSF_DNA-bd. IPR001789. Sig_transdc_resp-reg_receiver. IPR014402. Sig_transdc_resp-reg_SKN7. IPR011991. WHTH_DNA-bd_dom. [Graphical view]
Pfam	PF00447. HSF_DNA-bind. 1 hit. PF00072. Response_reg. 1 hit. [Graphical view]
PIRSF	PIRSF002595. RR_SKN7. 1 hit.
PRINTS	PR00056. HSFDOMAIN.
SMART	SM00415. HSF. 1 hit. SM00448. REC. 1 hit. [Graphical view]
SUPFAM	SSF52172. CheY_like. 1 hit.
PROSITE	PS00434. HSF_DOMAIN. 1 hit. PS50110. RESPONSE_REGULATORY. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	982535.

Entry information

Entry name

SKN7_YEAST

Accession

Primary (citable) accession number: P38889
Secondary accession number(s): D3DLF5, E9P959, P39747

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1995
Last sequence update:	February 1, 1995
Last modified:	May 1, 2013
This is version 120 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program

Relevant documents

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

Yeast chromosome VIII

Yeast (Saccharomyces cerevisiae) chromosome VIII: entries and gene names

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Amino acid modifications

Experimental info

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents