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P38889

- SKN7_YEAST

UniProt

P38889 - SKN7_YEAST

Protein

Transcription factor SKN7

Gene

SKN7

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 133 (01 Oct 2014)
      Sequence version 1 (01 Feb 1995)
      Previous versions | rss
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    Functioni

    Transcription factor that is part of a SLN1-YPD1-SKN7 two-component regulatory system, which controls gene expression in response to changes in the osmolarity of the extracellular environment. Under low osmotic conditions, phosphorylated and activated by the phosphorelay intermediate protein YPD1. Also activated in response to oxidative stress, independent on the two-component regulatory system. Regulates heat shock genes in response to oxidative stress and genes involved in cell wall integrity in response to osmotic changes.4 Publications

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    DNA bindingi86 – 190105By similarityAdd
    BLAST

    GO - Molecular functioni

    1. phosphorelay response regulator activity Source: SGD
    2. sequence-specific DNA binding Source: InterPro
    3. sequence-specific DNA binding transcription factor activity Source: SGD

    GO - Biological processi

    1. intracellular signal transduction Source: GOC
    2. regulation of cell size Source: SGD
    3. regulation of transcription from RNA polymerase II promoter in response to oxidative stress Source: SGD
    4. response to osmotic stress Source: SGD
    5. response to singlet oxygen Source: SGD
    6. transcription, DNA-templated Source: SGD

    Keywords - Biological processi

    Transcription, Transcription regulation, Two-component regulatory system

    Keywords - Ligandi

    DNA-binding

    Enzyme and pathway databases

    BioCyciYEAST:G3O-31232-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Transcription factor SKN7
    Alternative name(s):
    Peroxide sensitivity protein 9
    Gene namesi
    Name:SKN7
    Synonyms:BRY1, POS9
    Ordered Locus Names:YHR206W
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome VIII

    Organism-specific databases

    CYGDiYHR206w.
    SGDiS000001249. SKN7.

    Subcellular locationi

    Nucleus 2 Publications

    GO - Cellular componenti

    1. cytosol Source: SGD
    2. nucleus Source: SGD

    Keywords - Cellular componenti

    Nucleus

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi427 – 4271D → A: No activity. 2 Publications
    Mutagenesisi427 – 4271D → E: Augments activity. 2 Publications
    Mutagenesisi427 – 4271D → N: Diminishes activity. 2 Publications
    Mutagenesisi427 – 4271D → R: No activity. 2 Publications

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 622622Transcription factor SKN7PRO_0000081404Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei427 – 42714-aspartylphosphate1 PublicationPROSITE-ProRule annotation

    Post-translational modificationi

    The phosphorelay mechanism involves the sequential transfer of a phosphate group from 'His-576' (H1) to 'Asp-1144' (D1) of SLN1, then to 'His-64' (H2) of YPD1 and finally to Asp-427 (D2) of SKN7.

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    MaxQBiP38889.
    PaxDbiP38889.
    PeptideAtlasiP38889.

    Expressioni

    Gene expression databases

    GenevestigatoriP38889.

    Interactioni

    Protein-protein interaction databases

    BioGridi36640. 198 interactions.
    DIPiDIP-2403N.
    IntActiP38889. 8 interactions.
    MINTiMINT-4489442.

    Structurei

    3D structure databases

    ProteinModelPortaliP38889.
    SMRiP38889. Positions 87-171, 379-535.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini378 – 492115Response regulatoryPROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Belongs to the SKN7 family.Curated
    Contains 1 response regulatory domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiCOG0784.
    GeneTreeiENSGT00610000087034.
    HOGENOMiHOG000248776.
    KOiK15859.
    OMAiNISSANA.
    OrthoDBiEOG7ZKSNN.

    Family and domain databases

    Gene3Di1.10.10.10. 1 hit.
    InterProiIPR011006. CheY-like_superfamily.
    IPR000232. HSF_DNA-bd.
    IPR027725. HSF_fam.
    IPR001789. Sig_transdc_resp-reg_receiver.
    IPR014402. Sig_transdc_resp-reg_Skn7.
    IPR027718. TF_Skn7.
    IPR011991. WHTH_DNA-bd_dom.
    [Graphical view]
    PANTHERiPTHR10015. PTHR10015. 1 hit.
    PTHR10015:SF145. PTHR10015:SF145. 1 hit.
    PfamiPF00447. HSF_DNA-bind. 1 hit.
    PF00072. Response_reg. 1 hit.
    [Graphical view]
    PIRSFiPIRSF002595. RR_SKN7. 1 hit.
    PRINTSiPR00056. HSFDOMAIN.
    SMARTiSM00415. HSF. 1 hit.
    SM00448. REC. 1 hit.
    [Graphical view]
    SUPFAMiSSF52172. SSF52172. 1 hit.
    PROSITEiPS00434. HSF_DOMAIN. 1 hit.
    PS50110. RESPONSE_REGULATORY. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P38889-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSFSTINSNV NKTTGDSNNN TTENSSTADL LGMDLLQSGP RLMNTMQPNN    50
    SSDMLHINNK TNNVQQPAGN TNISSANAGA KAPANEFVRK LFRILENNEY 100
    PDIVTWTENG KSFVVLDTGK FTTHILPNHF KHSNFASFVR QLNKYDFHKV 150
    KRSPEERQRC KYGEQSWEFQ HPEFRVHYGK GLDNIKRKIP AQRKVLLDES 200
    QKALLHFNSE GTNPNNPSGS LLNESTTELL LSNTVSKDAF GNLRRRVDKL 250
    QKELDMSKME SYATKVELQK LNSKYNTVIE SLITFKTINE NLLNNFNTLC 300
    STLANNGIEV PIFGDNGNRN PTGNTNPATT TAIQSNNNTN NASPATSTVS 350
    LQLPNLPDQN SLTPNAQNNT VTLRKGFHVL LVEDDAVSIQ LCSKFLRKYG 400
    CTVQVVSDGL SAISTLEKYR YDLVLMDIVM PNLDGATATS IVRSFDNETP 450
    IIAMTGNIMN QDLITYLQHG MNDILAKPFT RDDLHSILIR YLKDRIPLCE 500
    QQLPPRNSSP QTHSNTNTAN SNPNTINEQS LAMLPQDNPS TTTPVTPGAS 550
    ISSAQHVQQG QQEQQHQIFH AQQQQQHHNA IANARSDVAI PNLEHEINTV 600
    PHSSMGSTPQ LPQSTLQENQ LS 622
    Length:622
    Mass (Da):69,202
    Last modified:February 1, 1995 - v1
    Checksum:i4C732FD66E326742
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti45 – 451T → A in AAU09745. (PubMed:17322287)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U00485 Unassigned DNA. Translation: AAC48911.1.
    X83031 Genomic DNA. Translation: CAA58143.1.
    U00029 Genomic DNA. Translation: AAB69734.1.
    AY723828 Genomic DNA. Translation: AAU09745.1.
    BK006934 Genomic DNA. Translation: DAA06899.1.
    PIRiA49344.
    RefSeqiNP_012076.3. NM_001179337.3.

    Genome annotation databases

    EnsemblFungiiYHR206W; YHR206W; YHR206W.
    GeneIDi856613.
    KEGGisce:YHR206W.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U00485 Unassigned DNA. Translation: AAC48911.1 .
    X83031 Genomic DNA. Translation: CAA58143.1 .
    U00029 Genomic DNA. Translation: AAB69734.1 .
    AY723828 Genomic DNA. Translation: AAU09745.1 .
    BK006934 Genomic DNA. Translation: DAA06899.1 .
    PIRi A49344.
    RefSeqi NP_012076.3. NM_001179337.3.

    3D structure databases

    ProteinModelPortali P38889.
    SMRi P38889. Positions 87-171, 379-535.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 36640. 198 interactions.
    DIPi DIP-2403N.
    IntActi P38889. 8 interactions.
    MINTi MINT-4489442.

    Chemistry

    ChEMBLi CHEMBL2146314.

    Proteomic databases

    MaxQBi P38889.
    PaxDbi P38889.
    PeptideAtlasi P38889.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YHR206W ; YHR206W ; YHR206W .
    GeneIDi 856613.
    KEGGi sce:YHR206W.

    Organism-specific databases

    CYGDi YHR206w.
    SGDi S000001249. SKN7.

    Phylogenomic databases

    eggNOGi COG0784.
    GeneTreei ENSGT00610000087034.
    HOGENOMi HOG000248776.
    KOi K15859.
    OMAi NISSANA.
    OrthoDBi EOG7ZKSNN.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-31232-MONOMER.

    Miscellaneous databases

    NextBioi 982535.

    Gene expression databases

    Genevestigatori P38889.

    Family and domain databases

    Gene3Di 1.10.10.10. 1 hit.
    InterProi IPR011006. CheY-like_superfamily.
    IPR000232. HSF_DNA-bd.
    IPR027725. HSF_fam.
    IPR001789. Sig_transdc_resp-reg_receiver.
    IPR014402. Sig_transdc_resp-reg_Skn7.
    IPR027718. TF_Skn7.
    IPR011991. WHTH_DNA-bd_dom.
    [Graphical view ]
    PANTHERi PTHR10015. PTHR10015. 1 hit.
    PTHR10015:SF145. PTHR10015:SF145. 1 hit.
    Pfami PF00447. HSF_DNA-bind. 1 hit.
    PF00072. Response_reg. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF002595. RR_SKN7. 1 hit.
    PRINTSi PR00056. HSFDOMAIN.
    SMARTi SM00415. HSF. 1 hit.
    SM00448. REC. 1 hit.
    [Graphical view ]
    SUPFAMi SSF52172. SSF52172. 1 hit.
    PROSITEi PS00434. HSF_DOMAIN. 1 hit.
    PS50110. RESPONSE_REGULATORY. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "SKN7, a yeast multicopy suppressor of a mutation affecting cell wall beta-glucan assembly, encodes a product with domains homologous to prokaryotic two-component regulators and to heat shock transcription factors."
      Brown J.L., North S., Bussey H.
      J. Bacteriol. 175:6908-6915(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE.
    2. "The response regulator-like protein Pos9/Skn7 of Saccharomyces cerevisiae is involved in oxidative stress resistance."
      Krems B., Charizanis C., Entian K.-D.
      Curr. Genet. 29:327-334(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, MUTAGENESIS OF ASP-427.
    3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    4. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    5. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    6. "Yeast Skn7p functions in a eukaryotic two-component regulatory pathway."
      Brown J.L., Bussey H., Stewart R.C.
      EMBO J. 13:5186-5194(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, MUTAGENESIS OF ASP-427.
    7. "The yeast histidine protein kinase, Sln1p, mediates phosphotransfer to two response regulators, Ssk1p and Skn7p."
      Li S., Ault A., Malone C.L., Raitt D., Dean S., Johnston L.H., Deschenes R.J., Fassler J.S.
      EMBO J. 17:6952-6962(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, PHOSPHORYLATION AT ASP-427.
    8. "The Skn7 response regulator of Saccharomyces cerevisiae interacts with Hsf1 in vivo and is required for the induction of heat shock genes by oxidative stress."
      Raitt D.C., Johnson A.L., Erkine A.M., Makino K., Morgan B., Gross D.S., Johnston L.H.
      Mol. Biol. Cell 11:2335-2347(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, SUBCELLULAR LOCATION.
    9. "Saccharomyces cerevisiae histidine phosphotransferase Ypd1p shuttles between the nucleus and cytoplasm for SLN1-dependent phosphorylation of Ssk1p and Skn7p."
      Lu J.M.-Y., Deschenes R.J., Fassler J.S.
      Eukaryot. Cell 2:1304-1314(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBCELLULAR LOCATION.
    10. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
    11. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
      Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
      Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiSKN7_YEAST
    AccessioniPrimary (citable) accession number: P38889
    Secondary accession number(s): D3DLF5, E9P959, P39747
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 1, 1995
    Last sequence update: February 1, 1995
    Last modified: October 1, 2014
    This is version 133 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 2570 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families
    2. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    3. Yeast chromosome VIII
      Yeast (Saccharomyces cerevisiae) chromosome VIII: entries and gene names

    External Data

    Dasty 3