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Protein

Transcription factor SKN7

Gene

SKN7

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Transcription factor that is part of a SLN1-YPD1-SKN7 two-component regulatory system, which controls gene expression in response to changes in the osmolarity of the extracellular environment. Under low osmotic conditions, phosphorylated and activated by the phosphorelay intermediate protein YPD1. Also activated in response to oxidative stress, independent on the two-component regulatory system. Regulates heat shock genes in response to oxidative stress and genes involved in cell wall integrity in response to osmotic changes.4 Publications

Miscellaneous

Present with 2570 molecules/cell in log phase SD medium.1 Publication

GO - Molecular functioni

  • DNA binding transcription factor activity Source: SGD
  • phosphorelay response regulator activity Source: SGD
  • sequence-specific DNA binding Source: InterPro

GO - Biological processi

  • regulation of cell size Source: SGD
  • regulation of endoplasmic reticulum unfolded protein response Source: SGD
  • regulation of transcription from RNA polymerase II promoter in response to oxidative stress Source: SGD
  • response to osmotic stress Source: SGD
  • response to singlet oxygen Source: SGD
  • transcription, DNA-templated Source: SGD

Keywordsi

Molecular functionDNA-binding
Biological processTranscription, Transcription regulation, Two-component regulatory system

Enzyme and pathway databases

BioCyciYEAST:G3O-31232-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Transcription factor SKN7
Alternative name(s):
Peroxide sensitivity protein 9
Gene namesi
Name:SKN7
Synonyms:BRY1, POS9
Ordered Locus Names:YHR206W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome VIII

Organism-specific databases

EuPathDBiFungiDB:YHR206W
SGDiS000001249 SKN7

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi427D → A: No activity. 2 Publications1
Mutagenesisi427D → E: Augments activity. 2 Publications1
Mutagenesisi427D → N: Diminishes activity. 2 Publications1
Mutagenesisi427D → R: No activity. 2 Publications1

Chemistry databases

ChEMBLiCHEMBL2146314

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000814041 – 622Transcription factor SKN7Add BLAST622

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei4274-aspartylphosphatePROSITE-ProRule annotation1 Publication1

Post-translational modificationi

The phosphorelay mechanism involves the sequential transfer of a phosphate group from 'His-576' (H1) to 'Asp-1144' (D1) of SLN1, then to 'His-64' (H2) of YPD1 and finally to Asp-427 (D2) of SKN7.

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP38889
PaxDbiP38889
PRIDEiP38889

PTM databases

iPTMnetiP38889

Interactioni

Subunit structurei

Homotrimer.By similarity

Protein-protein interaction databases

BioGridi36640, 259 interactors
DIPiDIP-2403N
IntActiP38889, 8 interactors
STRINGi4932.YHR206W

Structurei

3D structure databases

ProteinModelPortaliP38889
SMRiP38889
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini378 – 492Response regulatoryPROSITE-ProRule annotationAdd BLAST115

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni84 – 190DNA-binding domainBy similarityAdd BLAST107
Regioni212 – 303Hydrophobic repeat HR-A/BBy similarityAdd BLAST92

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili240 – 260Sequence analysisAdd BLAST21

Domaini

Homotrimerization occurs through formation of a three-stranded coiled-coil structure generated by intermolecular interactions between HR-A/B regions allowing DNA-binding activity.By similarity

Sequence similaritiesi

Belongs to the SKN7 family.Curated

Keywords - Domaini

Coiled coil

Phylogenomic databases

GeneTreeiENSGT00610000087034
HOGENOMiHOG000248776
InParanoidiP38889
KOiK15859
OMAiATIEFAQ
OrthoDBiEOG092C2G9O

Family and domain databases

CDDicd00156 REC, 1 hit
Gene3Di1.10.10.10, 1 hit
InterProiView protein in InterPro
IPR011006 CheY-like_superfamily
IPR000232 HSF_DNA-bd
IPR027725 HSF_fam
IPR001789 Sig_transdc_resp-reg_receiver
IPR014402 Sig_transdc_resp-reg_Skn7
IPR036388 WH-like_DNA-bd_sf
IPR036390 WH_DNA-bd_sf
PANTHERiPTHR10015 PTHR10015, 3 hits
PfamiView protein in Pfam
PF00447 HSF_DNA-bind, 1 hit
PF00072 Response_reg, 1 hit
PIRSFiPIRSF002595 RR_SKN7, 1 hit
PRINTSiPR00056 HSFDOMAIN
SMARTiView protein in SMART
SM00415 HSF, 1 hit
SM00448 REC, 1 hit
SUPFAMiSSF46785 SSF46785, 1 hit
SSF52172 SSF52172, 1 hit
PROSITEiView protein in PROSITE
PS00434 HSF_DOMAIN, 1 hit
PS50110 RESPONSE_REGULATORY, 1 hit

Sequencei

Sequence statusi: Complete.

P38889-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSFSTINSNV NKTTGDSNNN TTENSSTADL LGMDLLQSGP RLMNTMQPNN
60 70 80 90 100
SSDMLHINNK TNNVQQPAGN TNISSANAGA KAPANEFVRK LFRILENNEY
110 120 130 140 150
PDIVTWTENG KSFVVLDTGK FTTHILPNHF KHSNFASFVR QLNKYDFHKV
160 170 180 190 200
KRSPEERQRC KYGEQSWEFQ HPEFRVHYGK GLDNIKRKIP AQRKVLLDES
210 220 230 240 250
QKALLHFNSE GTNPNNPSGS LLNESTTELL LSNTVSKDAF GNLRRRVDKL
260 270 280 290 300
QKELDMSKME SYATKVELQK LNSKYNTVIE SLITFKTINE NLLNNFNTLC
310 320 330 340 350
STLANNGIEV PIFGDNGNRN PTGNTNPATT TAIQSNNNTN NASPATSTVS
360 370 380 390 400
LQLPNLPDQN SLTPNAQNNT VTLRKGFHVL LVEDDAVSIQ LCSKFLRKYG
410 420 430 440 450
CTVQVVSDGL SAISTLEKYR YDLVLMDIVM PNLDGATATS IVRSFDNETP
460 470 480 490 500
IIAMTGNIMN QDLITYLQHG MNDILAKPFT RDDLHSILIR YLKDRIPLCE
510 520 530 540 550
QQLPPRNSSP QTHSNTNTAN SNPNTINEQS LAMLPQDNPS TTTPVTPGAS
560 570 580 590 600
ISSAQHVQQG QQEQQHQIFH AQQQQQHHNA IANARSDVAI PNLEHEINTV
610 620
PHSSMGSTPQ LPQSTLQENQ LS
Length:622
Mass (Da):69,202
Last modified:February 1, 1995 - v1
Checksum:i4C732FD66E326742
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti45T → A in AAU09745 (PubMed:17322287).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00485 Unassigned DNA Translation: AAC48911.1
X83031 Genomic DNA Translation: CAA58143.1
U00029 Genomic DNA Translation: AAB69734.1
AY723828 Genomic DNA Translation: AAU09745.1
BK006934 Genomic DNA Translation: DAA06899.1
PIRiA49344
RefSeqiNP_012076.3, NM_001179337.3

Genome annotation databases

EnsemblFungiiYHR206W; YHR206W; YHR206W
GeneIDi856613
KEGGisce:YHR206W

Similar proteinsi

Entry informationi

Entry nameiSKN7_YEAST
AccessioniPrimary (citable) accession number: P38889
Secondary accession number(s): D3DLF5, E9P959, P39747
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: February 1, 1995
Last modified: May 23, 2018
This is version 164 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome VIII
    Yeast (Saccharomyces cerevisiae) chromosome VIII: entries and gene names

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