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P38889 (SKN7_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 130. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Transcription factor SKN7
Alternative name(s):
Peroxide sensitivity protein 9
Gene names
Name:SKN7
Synonyms:BRY1, POS9
Ordered Locus Names:YHR206W
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length622 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Transcription factor that is part of a SLN1-YPD1-SKN7 two-component regulatory system, which controls gene expression in response to changes in the osmolarity of the extracellular environment. Under low osmotic conditions, phosphorylated and activated by the phosphorelay intermediate protein YPD1. Also activated in response to oxidative stress, independent on the two-component regulatory system. Regulates heat shock genes in response to oxidative stress and genes involved in cell wall integrity in response to osmotic changes. Ref.2 Ref.6 Ref.7 Ref.8

Subcellular location

Nucleus Ref.8 Ref.9.

Post-translational modification

The phosphorelay mechanism involves the sequential transfer of a phosphate group from 'His-576' (H1) to 'Asp-1144' (D1) of SLN1, then to 'His-64' (H2) of YPD1 and finally to Asp-427 (D2) of SKN7.

Miscellaneous

Present with 2570 molecules/cell in log phase SD medium.

Sequence similarities

Belongs to the SKN7 family.

Contains 1 response regulatory domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 622622Transcription factor SKN7
PRO_0000081404

Regions

Domain378 – 492115Response regulatory
DNA binding86 – 190105 By similarity

Amino acid modifications

Modified residue42714-aspartylphosphate

Experimental info

Mutagenesis4271D → A: No activity. Ref.2 Ref.6
Mutagenesis4271D → E: Augments activity. Ref.2 Ref.6
Mutagenesis4271D → N: Diminishes activity. Ref.2 Ref.6
Mutagenesis4271D → R: No activity. Ref.2 Ref.6
Sequence conflict451T → A in AAU09745. Ref.5

Sequences

Sequence LengthMass (Da)Tools
P38889 [UniParc].

Last modified February 1, 1995. Version 1.
Checksum: 4C732FD66E326742

FASTA62269,202
        10         20         30         40         50         60 
MSFSTINSNV NKTTGDSNNN TTENSSTADL LGMDLLQSGP RLMNTMQPNN SSDMLHINNK 

        70         80         90        100        110        120 
TNNVQQPAGN TNISSANAGA KAPANEFVRK LFRILENNEY PDIVTWTENG KSFVVLDTGK 

       130        140        150        160        170        180 
FTTHILPNHF KHSNFASFVR QLNKYDFHKV KRSPEERQRC KYGEQSWEFQ HPEFRVHYGK 

       190        200        210        220        230        240 
GLDNIKRKIP AQRKVLLDES QKALLHFNSE GTNPNNPSGS LLNESTTELL LSNTVSKDAF 

       250        260        270        280        290        300 
GNLRRRVDKL QKELDMSKME SYATKVELQK LNSKYNTVIE SLITFKTINE NLLNNFNTLC 

       310        320        330        340        350        360 
STLANNGIEV PIFGDNGNRN PTGNTNPATT TAIQSNNNTN NASPATSTVS LQLPNLPDQN 

       370        380        390        400        410        420 
SLTPNAQNNT VTLRKGFHVL LVEDDAVSIQ LCSKFLRKYG CTVQVVSDGL SAISTLEKYR 

       430        440        450        460        470        480 
YDLVLMDIVM PNLDGATATS IVRSFDNETP IIAMTGNIMN QDLITYLQHG MNDILAKPFT 

       490        500        510        520        530        540 
RDDLHSILIR YLKDRIPLCE QQLPPRNSSP QTHSNTNTAN SNPNTINEQS LAMLPQDNPS 

       550        560        570        580        590        600 
TTTPVTPGAS ISSAQHVQQG QQEQQHQIFH AQQQQQHHNA IANARSDVAI PNLEHEINTV 

       610        620 
PHSSMGSTPQ LPQSTLQENQ LS 

« Hide

References

« Hide 'large scale' references
[1]"SKN7, a yeast multicopy suppressor of a mutation affecting cell wall beta-glucan assembly, encodes a product with domains homologous to prokaryotic two-component regulators and to heat shock transcription factors."
Brown J.L., North S., Bussey H.
J. Bacteriol. 175:6908-6915(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE.
[2]"The response regulator-like protein Pos9/Skn7 of Saccharomyces cerevisiae is involved in oxidative stress resistance."
Krems B., Charizanis C., Entian K.-D.
Curr. Genet. 29:327-334(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, MUTAGENESIS OF ASP-427.
[3]"Complete nucleotide sequence of Saccharomyces cerevisiae chromosome VIII."
Johnston M., Andrews S., Brinkman R., Cooper J., Ding H., Dover J., Du Z., Favello A., Fulton L., Gattung S., Geisel C., Kirsten J., Kucaba T., Hillier L.W., Jier M., Johnston L., Langston Y., Latreille P. expand/collapse author list , Louis E.J., Macri C., Mardis E., Menezes S., Mouser L., Nhan M., Rifkin L., Riles L., St Peter H., Trevaskis E., Vaughan K., Vignati D., Wilcox L., Wohldman P., Waterston R., Wilson R., Vaudin M.
Science 265:2077-2082(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[4]Saccharomyces Genome Database
Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[5]"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae."
Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. expand/collapse author list , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J.
Genome Res. 17:536-543(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[6]"Yeast Skn7p functions in a eukaryotic two-component regulatory pathway."
Brown J.L., Bussey H., Stewart R.C.
EMBO J. 13:5186-5194(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, MUTAGENESIS OF ASP-427.
[7]"The yeast histidine protein kinase, Sln1p, mediates phosphotransfer to two response regulators, Ssk1p and Skn7p."
Li S., Ault A., Malone C.L., Raitt D., Dean S., Johnston L.H., Deschenes R.J., Fassler J.S.
EMBO J. 17:6952-6962(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, PHOSPHORYLATION AT ASP-427.
[8]"The Skn7 response regulator of Saccharomyces cerevisiae interacts with Hsf1 in vivo and is required for the induction of heat shock genes by oxidative stress."
Raitt D.C., Johnson A.L., Erkine A.M., Makino K., Morgan B., Gross D.S., Johnston L.H.
Mol. Biol. Cell 11:2335-2347(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, SUBCELLULAR LOCATION.
[9]"Saccharomyces cerevisiae histidine phosphotransferase Ypd1p shuttles between the nucleus and cytoplasm for SLN1-dependent phosphorylation of Ssk1p and Skn7p."
Lu J.M.-Y., Deschenes R.J., Fassler J.S.
Eukaryot. Cell 2:1304-1314(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION.
[10]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[11]"A multidimensional chromatography technology for in-depth phosphoproteome analysis."
Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U00485 Unassigned DNA. Translation: AAC48911.1.
X83031 Genomic DNA. Translation: CAA58143.1.
U00029 Genomic DNA. Translation: AAB69734.1.
AY723828 Genomic DNA. Translation: AAU09745.1.
BK006934 Genomic DNA. Translation: DAA06899.1.
PIRA49344.
RefSeqNP_012076.3. NM_001179337.3.

3D structure databases

ProteinModelPortalP38889.
SMRP38889. Positions 87-171, 379-535.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid36640. 198 interactions.
DIPDIP-2403N.
IntActP38889. 8 interactions.
MINTMINT-4489442.

Chemistry

ChEMBLCHEMBL2146314.

Proteomic databases

PaxDbP38889.
PeptideAtlasP38889.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYHR206W; YHR206W; YHR206W.
GeneID856613.
KEGGsce:YHR206W.

Organism-specific databases

CYGDYHR206w.
SGDS000001249. SKN7.

Phylogenomic databases

eggNOGCOG0784.
GeneTreeENSGT00610000087034.
HOGENOMHOG000248776.
KOK15859.
OMANISSANA.
OrthoDBEOG7ZKSNN.

Enzyme and pathway databases

BioCycYEAST:G3O-31232-MONOMER.

Gene expression databases

GenevestigatorP38889.

Family and domain databases

Gene3D1.10.10.10. 1 hit.
InterProIPR011006. CheY-like_superfamily.
IPR000232. HSF_DNA-bd.
IPR027725. HSF_fam.
IPR001789. Sig_transdc_resp-reg_receiver.
IPR014402. Sig_transdc_resp-reg_Skn7.
IPR027718. TF_Skn7.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PANTHERPTHR10015. PTHR10015. 1 hit.
PTHR10015:SF6. PTHR10015:SF6. 1 hit.
PfamPF00447. HSF_DNA-bind. 1 hit.
PF00072. Response_reg. 1 hit.
[Graphical view]
PIRSFPIRSF002595. RR_SKN7. 1 hit.
PRINTSPR00056. HSFDOMAIN.
SMARTSM00415. HSF. 1 hit.
SM00448. REC. 1 hit.
[Graphical view]
SUPFAMSSF52172. SSF52172. 1 hit.
PROSITEPS00434. HSF_DOMAIN. 1 hit.
PS50110. RESPONSE_REGULATORY. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio982535.

Entry information

Entry nameSKN7_YEAST
AccessionPrimary (citable) accession number: P38889
Secondary accession number(s): D3DLF5, E9P959, P39747
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: February 1, 1995
Last modified: April 16, 2014
This is version 130 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome VIII

Yeast (Saccharomyces cerevisiae) chromosome VIII: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families