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Protein

Transcription factor SKN7

Gene

SKN7

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Transcription factor that is part of a SLN1-YPD1-SKN7 two-component regulatory system, which controls gene expression in response to changes in the osmolarity of the extracellular environment. Under low osmotic conditions, phosphorylated and activated by the phosphorelay intermediate protein YPD1. Also activated in response to oxidative stress, independent on the two-component regulatory system. Regulates heat shock genes in response to oxidative stress and genes involved in cell wall integrity in response to osmotic changes.4 Publications

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
DNA bindingi86 – 190105By similarityAdd
BLAST

GO - Molecular functioni

  • phosphorelay response regulator activity Source: SGD
  • sequence-specific DNA binding Source: InterPro
  • sequence-specific DNA binding transcription factor activity Source: SGD

GO - Biological processi

  • regulation of cell size Source: SGD
  • regulation of transcription from RNA polymerase II promoter in response to oxidative stress Source: SGD
  • response to osmotic stress Source: SGD
  • response to singlet oxygen Source: SGD
  • transcription, DNA-templated Source: SGD
Complete GO annotation...

Keywords - Biological processi

Transcription, Transcription regulation, Two-component regulatory system

Keywords - Ligandi

DNA-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-31232-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Transcription factor SKN7
Alternative name(s):
Peroxide sensitivity protein 9
Gene namesi
Name:SKN7
Synonyms:BRY1, POS9
Ordered Locus Names:YHR206W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311 Componenti: Chromosome VIII

Organism-specific databases

CYGDiYHR206w.
EuPathDBiFungiDB:YHR206W.
SGDiS000001249. SKN7.

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: SGD
  • nucleus Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi427 – 4271D → A: No activity. 2 Publications
Mutagenesisi427 – 4271D → E: Augments activity. 2 Publications
Mutagenesisi427 – 4271D → N: Diminishes activity. 2 Publications
Mutagenesisi427 – 4271D → R: No activity. 2 Publications

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 622622Transcription factor SKN7PRO_0000081404Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei427 – 42714-aspartylphosphatePROSITE-ProRule annotation1 Publication

Post-translational modificationi

The phosphorelay mechanism involves the sequential transfer of a phosphate group from 'His-576' (H1) to 'Asp-1144' (D1) of SLN1, then to 'His-64' (H2) of YPD1 and finally to Asp-427 (D2) of SKN7.

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP38889.
PaxDbiP38889.
PeptideAtlasiP38889.

Interactioni

Protein-protein interaction databases

BioGridi36640. 199 interactions.
DIPiDIP-2403N.
IntActiP38889. 8 interactions.
MINTiMINT-4489442.

Structurei

3D structure databases

ProteinModelPortaliP38889.
SMRiP38889. Positions 87-171, 379-535.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini378 – 492115Response regulatoryPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the SKN7 family.Curated
Contains 1 response regulatory domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiCOG0784.
GeneTreeiENSGT00610000087034.
HOGENOMiHOG000248776.
InParanoidiP38889.
KOiK15859.
OMAiKFTTHIL.
OrthoDBiEOG7ZKSNN.

Family and domain databases

Gene3Di1.10.10.10. 1 hit.
InterProiIPR011006. CheY-like_superfamily.
IPR000232. HSF_DNA-bd.
IPR027725. HSF_fam.
IPR001789. Sig_transdc_resp-reg_receiver.
IPR027718. TF_Skn7.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PANTHERiPTHR10015. PTHR10015. 1 hit.
PTHR10015:SF145. PTHR10015:SF145. 1 hit.
PfamiPF00447. HSF_DNA-bind. 1 hit.
PF00072. Response_reg. 1 hit.
[Graphical view]
PRINTSiPR00056. HSFDOMAIN.
SMARTiSM00415. HSF. 1 hit.
SM00448. REC. 1 hit.
[Graphical view]
SUPFAMiSSF52172. SSF52172. 1 hit.
PROSITEiPS00434. HSF_DOMAIN. 1 hit.
PS50110. RESPONSE_REGULATORY. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P38889-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSFSTINSNV NKTTGDSNNN TTENSSTADL LGMDLLQSGP RLMNTMQPNN
60 70 80 90 100
SSDMLHINNK TNNVQQPAGN TNISSANAGA KAPANEFVRK LFRILENNEY
110 120 130 140 150
PDIVTWTENG KSFVVLDTGK FTTHILPNHF KHSNFASFVR QLNKYDFHKV
160 170 180 190 200
KRSPEERQRC KYGEQSWEFQ HPEFRVHYGK GLDNIKRKIP AQRKVLLDES
210 220 230 240 250
QKALLHFNSE GTNPNNPSGS LLNESTTELL LSNTVSKDAF GNLRRRVDKL
260 270 280 290 300
QKELDMSKME SYATKVELQK LNSKYNTVIE SLITFKTINE NLLNNFNTLC
310 320 330 340 350
STLANNGIEV PIFGDNGNRN PTGNTNPATT TAIQSNNNTN NASPATSTVS
360 370 380 390 400
LQLPNLPDQN SLTPNAQNNT VTLRKGFHVL LVEDDAVSIQ LCSKFLRKYG
410 420 430 440 450
CTVQVVSDGL SAISTLEKYR YDLVLMDIVM PNLDGATATS IVRSFDNETP
460 470 480 490 500
IIAMTGNIMN QDLITYLQHG MNDILAKPFT RDDLHSILIR YLKDRIPLCE
510 520 530 540 550
QQLPPRNSSP QTHSNTNTAN SNPNTINEQS LAMLPQDNPS TTTPVTPGAS
560 570 580 590 600
ISSAQHVQQG QQEQQHQIFH AQQQQQHHNA IANARSDVAI PNLEHEINTV
610 620
PHSSMGSTPQ LPQSTLQENQ LS
Length:622
Mass (Da):69,202
Last modified:February 1, 1995 - v1
Checksum:i4C732FD66E326742
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti45 – 451T → A in AAU09745 (PubMed:17322287).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00485 Unassigned DNA. Translation: AAC48911.1.
X83031 Genomic DNA. Translation: CAA58143.1.
U00029 Genomic DNA. Translation: AAB69734.1.
AY723828 Genomic DNA. Translation: AAU09745.1.
BK006934 Genomic DNA. Translation: DAA06899.1.
PIRiA49344.
RefSeqiNP_012076.3. NM_001179337.3.

Genome annotation databases

EnsemblFungiiYHR206W; YHR206W; YHR206W.
GeneIDi856613.
KEGGisce:YHR206W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00485 Unassigned DNA. Translation: AAC48911.1.
X83031 Genomic DNA. Translation: CAA58143.1.
U00029 Genomic DNA. Translation: AAB69734.1.
AY723828 Genomic DNA. Translation: AAU09745.1.
BK006934 Genomic DNA. Translation: DAA06899.1.
PIRiA49344.
RefSeqiNP_012076.3. NM_001179337.3.

3D structure databases

ProteinModelPortaliP38889.
SMRiP38889. Positions 87-171, 379-535.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi36640. 199 interactions.
DIPiDIP-2403N.
IntActiP38889. 8 interactions.
MINTiMINT-4489442.

Chemistry

ChEMBLiCHEMBL2146314.

Proteomic databases

MaxQBiP38889.
PaxDbiP38889.
PeptideAtlasiP38889.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYHR206W; YHR206W; YHR206W.
GeneIDi856613.
KEGGisce:YHR206W.

Organism-specific databases

CYGDiYHR206w.
EuPathDBiFungiDB:YHR206W.
SGDiS000001249. SKN7.

Phylogenomic databases

eggNOGiCOG0784.
GeneTreeiENSGT00610000087034.
HOGENOMiHOG000248776.
InParanoidiP38889.
KOiK15859.
OMAiKFTTHIL.
OrthoDBiEOG7ZKSNN.

Enzyme and pathway databases

BioCyciYEAST:G3O-31232-MONOMER.

Miscellaneous databases

NextBioi982535.
PROiP38889.

Family and domain databases

Gene3Di1.10.10.10. 1 hit.
InterProiIPR011006. CheY-like_superfamily.
IPR000232. HSF_DNA-bd.
IPR027725. HSF_fam.
IPR001789. Sig_transdc_resp-reg_receiver.
IPR027718. TF_Skn7.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PANTHERiPTHR10015. PTHR10015. 1 hit.
PTHR10015:SF145. PTHR10015:SF145. 1 hit.
PfamiPF00447. HSF_DNA-bind. 1 hit.
PF00072. Response_reg. 1 hit.
[Graphical view]
PRINTSiPR00056. HSFDOMAIN.
SMARTiSM00415. HSF. 1 hit.
SM00448. REC. 1 hit.
[Graphical view]
SUPFAMiSSF52172. SSF52172. 1 hit.
PROSITEiPS00434. HSF_DOMAIN. 1 hit.
PS50110. RESPONSE_REGULATORY. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "SKN7, a yeast multicopy suppressor of a mutation affecting cell wall beta-glucan assembly, encodes a product with domains homologous to prokaryotic two-component regulators and to heat shock transcription factors."
    Brown J.L., North S., Bussey H.
    J. Bacteriol. 175:6908-6915(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE.
  2. "The response regulator-like protein Pos9/Skn7 of Saccharomyces cerevisiae is involved in oxidative stress resistance."
    Krems B., Charizanis C., Entian K.-D.
    Curr. Genet. 29:327-334(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, MUTAGENESIS OF ASP-427.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  4. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  5. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  6. "Yeast Skn7p functions in a eukaryotic two-component regulatory pathway."
    Brown J.L., Bussey H., Stewart R.C.
    EMBO J. 13:5186-5194(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, MUTAGENESIS OF ASP-427.
  7. "The yeast histidine protein kinase, Sln1p, mediates phosphotransfer to two response regulators, Ssk1p and Skn7p."
    Li S., Ault A., Malone C.L., Raitt D., Dean S., Johnston L.H., Deschenes R.J., Fassler J.S.
    EMBO J. 17:6952-6962(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, PHOSPHORYLATION AT ASP-427.
  8. "The Skn7 response regulator of Saccharomyces cerevisiae interacts with Hsf1 in vivo and is required for the induction of heat shock genes by oxidative stress."
    Raitt D.C., Johnson A.L., Erkine A.M., Makino K., Morgan B., Gross D.S., Johnston L.H.
    Mol. Biol. Cell 11:2335-2347(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION.
  9. "Saccharomyces cerevisiae histidine phosphotransferase Ypd1p shuttles between the nucleus and cytoplasm for SLN1-dependent phosphorylation of Ssk1p and Skn7p."
    Lu J.M.-Y., Deschenes R.J., Fassler J.S.
    Eukaryot. Cell 2:1304-1314(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION.
  10. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  11. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
    Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
    Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiSKN7_YEAST
AccessioniPrimary (citable) accession number: P38889
Secondary accession number(s): D3DLF5, E9P959, P39747
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: February 1, 1995
Last modified: July 22, 2015
This is version 139 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 2570 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome VIII
    Yeast (Saccharomyces cerevisiae) chromosome VIII: entries and gene names

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.