Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Transcription factor SKN7

Gene

SKN7

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Transcription factor that is part of a SLN1-YPD1-SKN7 two-component regulatory system, which controls gene expression in response to changes in the osmolarity of the extracellular environment. Under low osmotic conditions, phosphorylated and activated by the phosphorelay intermediate protein YPD1. Also activated in response to oxidative stress, independent on the two-component regulatory system. Regulates heat shock genes in response to oxidative stress and genes involved in cell wall integrity in response to osmotic changes.4 Publications

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
DNA bindingi86 – 190By similarityAdd BLAST105

GO - Molecular functioni

  • phosphorelay response regulator activity Source: SGD
  • sequence-specific DNA binding Source: InterPro
  • transcription factor activity, sequence-specific DNA binding Source: SGD

GO - Biological processi

  • regulation of cell size Source: SGD
  • regulation of endoplasmic reticulum unfolded protein response Source: SGD
  • regulation of transcription from RNA polymerase II promoter in response to oxidative stress Source: SGD
  • response to osmotic stress Source: SGD
  • response to singlet oxygen Source: SGD
  • transcription, DNA-templated Source: SGD
Complete GO annotation...

Keywords - Biological processi

Transcription, Transcription regulation, Two-component regulatory system

Keywords - Ligandi

DNA-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-31232-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Transcription factor SKN7
Alternative name(s):
Peroxide sensitivity protein 9
Gene namesi
Name:SKN7
Synonyms:BRY1, POS9
Ordered Locus Names:YHR206W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome VIII

Organism-specific databases

EuPathDBiFungiDB:YHR206W.
SGDiS000001249. SKN7.

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: SGD
  • nucleus Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi427D → A: No activity. 2 Publications1
Mutagenesisi427D → E: Augments activity. 2 Publications1
Mutagenesisi427D → N: Diminishes activity. 2 Publications1
Mutagenesisi427D → R: No activity. 2 Publications1

Chemistry databases

ChEMBLiCHEMBL2146314.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000814041 – 622Transcription factor SKN7Add BLAST622

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei4274-aspartylphosphatePROSITE-ProRule annotation1 Publication1

Post-translational modificationi

The phosphorelay mechanism involves the sequential transfer of a phosphate group from 'His-576' (H1) to 'Asp-1144' (D1) of SLN1, then to 'His-64' (H2) of YPD1 and finally to Asp-427 (D2) of SKN7.

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP38889.
PRIDEiP38889.

PTM databases

iPTMnetiP38889.

Interactioni

Protein-protein interaction databases

BioGridi36640. 202 interactors.
DIPiDIP-2403N.
IntActiP38889. 8 interactors.
MINTiMINT-4489442.

Structurei

3D structure databases

ProteinModelPortaliP38889.
SMRiP38889.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini378 – 492Response regulatoryPROSITE-ProRule annotationAdd BLAST115

Sequence similaritiesi

Belongs to the SKN7 family.Curated
Contains 1 response regulatory domain.PROSITE-ProRule annotation

Phylogenomic databases

GeneTreeiENSGT00610000087034.
HOGENOMiHOG000248776.
InParanoidiP38889.
KOiK15859.
OMAiSWEFQHP.
OrthoDBiEOG092C2G9O.

Family and domain databases

Gene3Di1.10.10.10. 1 hit.
InterProiIPR011006. CheY-like_superfamily.
IPR000232. HSF_DNA-bd.
IPR027725. HSF_fam.
IPR001789. Sig_transdc_resp-reg_receiver.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PANTHERiPTHR10015. PTHR10015. 2 hits.
PfamiPF00447. HSF_DNA-bind. 1 hit.
PF00072. Response_reg. 1 hit.
[Graphical view]
PRINTSiPR00056. HSFDOMAIN.
SMARTiSM00415. HSF. 1 hit.
SM00448. REC. 1 hit.
[Graphical view]
SUPFAMiSSF46785. SSF46785. 1 hit.
SSF52172. SSF52172. 1 hit.
PROSITEiPS00434. HSF_DOMAIN. 1 hit.
PS50110. RESPONSE_REGULATORY. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P38889-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSFSTINSNV NKTTGDSNNN TTENSSTADL LGMDLLQSGP RLMNTMQPNN
60 70 80 90 100
SSDMLHINNK TNNVQQPAGN TNISSANAGA KAPANEFVRK LFRILENNEY
110 120 130 140 150
PDIVTWTENG KSFVVLDTGK FTTHILPNHF KHSNFASFVR QLNKYDFHKV
160 170 180 190 200
KRSPEERQRC KYGEQSWEFQ HPEFRVHYGK GLDNIKRKIP AQRKVLLDES
210 220 230 240 250
QKALLHFNSE GTNPNNPSGS LLNESTTELL LSNTVSKDAF GNLRRRVDKL
260 270 280 290 300
QKELDMSKME SYATKVELQK LNSKYNTVIE SLITFKTINE NLLNNFNTLC
310 320 330 340 350
STLANNGIEV PIFGDNGNRN PTGNTNPATT TAIQSNNNTN NASPATSTVS
360 370 380 390 400
LQLPNLPDQN SLTPNAQNNT VTLRKGFHVL LVEDDAVSIQ LCSKFLRKYG
410 420 430 440 450
CTVQVVSDGL SAISTLEKYR YDLVLMDIVM PNLDGATATS IVRSFDNETP
460 470 480 490 500
IIAMTGNIMN QDLITYLQHG MNDILAKPFT RDDLHSILIR YLKDRIPLCE
510 520 530 540 550
QQLPPRNSSP QTHSNTNTAN SNPNTINEQS LAMLPQDNPS TTTPVTPGAS
560 570 580 590 600
ISSAQHVQQG QQEQQHQIFH AQQQQQHHNA IANARSDVAI PNLEHEINTV
610 620
PHSSMGSTPQ LPQSTLQENQ LS
Length:622
Mass (Da):69,202
Last modified:February 1, 1995 - v1
Checksum:i4C732FD66E326742
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti45T → A in AAU09745 (PubMed:17322287).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00485 Unassigned DNA. Translation: AAC48911.1.
X83031 Genomic DNA. Translation: CAA58143.1.
U00029 Genomic DNA. Translation: AAB69734.1.
AY723828 Genomic DNA. Translation: AAU09745.1.
BK006934 Genomic DNA. Translation: DAA06899.1.
PIRiA49344.
RefSeqiNP_012076.3. NM_001179337.3.

Genome annotation databases

EnsemblFungiiYHR206W; YHR206W; YHR206W.
GeneIDi856613.
KEGGisce:YHR206W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00485 Unassigned DNA. Translation: AAC48911.1.
X83031 Genomic DNA. Translation: CAA58143.1.
U00029 Genomic DNA. Translation: AAB69734.1.
AY723828 Genomic DNA. Translation: AAU09745.1.
BK006934 Genomic DNA. Translation: DAA06899.1.
PIRiA49344.
RefSeqiNP_012076.3. NM_001179337.3.

3D structure databases

ProteinModelPortaliP38889.
SMRiP38889.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi36640. 202 interactors.
DIPiDIP-2403N.
IntActiP38889. 8 interactors.
MINTiMINT-4489442.

Chemistry databases

ChEMBLiCHEMBL2146314.

PTM databases

iPTMnetiP38889.

Proteomic databases

MaxQBiP38889.
PRIDEiP38889.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYHR206W; YHR206W; YHR206W.
GeneIDi856613.
KEGGisce:YHR206W.

Organism-specific databases

EuPathDBiFungiDB:YHR206W.
SGDiS000001249. SKN7.

Phylogenomic databases

GeneTreeiENSGT00610000087034.
HOGENOMiHOG000248776.
InParanoidiP38889.
KOiK15859.
OMAiSWEFQHP.
OrthoDBiEOG092C2G9O.

Enzyme and pathway databases

BioCyciYEAST:G3O-31232-MONOMER.

Miscellaneous databases

PROiP38889.

Family and domain databases

Gene3Di1.10.10.10. 1 hit.
InterProiIPR011006. CheY-like_superfamily.
IPR000232. HSF_DNA-bd.
IPR027725. HSF_fam.
IPR001789. Sig_transdc_resp-reg_receiver.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PANTHERiPTHR10015. PTHR10015. 2 hits.
PfamiPF00447. HSF_DNA-bind. 1 hit.
PF00072. Response_reg. 1 hit.
[Graphical view]
PRINTSiPR00056. HSFDOMAIN.
SMARTiSM00415. HSF. 1 hit.
SM00448. REC. 1 hit.
[Graphical view]
SUPFAMiSSF46785. SSF46785. 1 hit.
SSF52172. SSF52172. 1 hit.
PROSITEiPS00434. HSF_DOMAIN. 1 hit.
PS50110. RESPONSE_REGULATORY. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiSKN7_YEAST
AccessioniPrimary (citable) accession number: P38889
Secondary accession number(s): D3DLF5, E9P959, P39747
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: February 1, 1995
Last modified: November 30, 2016
This is version 151 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 2570 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome VIII
    Yeast (Saccharomyces cerevisiae) chromosome VIII: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.