Reviewed,
UniProtKB/Swiss-Prot P38888 (MNL1_YEAST)
Last modified
November 24, 2009.
Version 76.
History...
Clusters with 100%,
90%,
50% identity |
Documents (4) |
Third-party data |
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Names and origin
| Protein names | Recommended name: ER degradation-enhancing alpha-mannosidase-like protein 1 | ||||||
| Gene names |
| ||||||
| Organism | Saccharomyces cerevisiae (Baker's yeast) [Complete proteome] | ||||||
| Taxonomic identifier | 4932 [NCBI] | ||||||
| Taxonomic lineage | Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces |
Protein attributes
| Sequence length | 796 AA. |
| Sequence status | Complete. |
| Sequence processing | The displayed sequence is further processed into a mature form. |
| Protein existence | Evidence at protein level. |
General annotation (Comments)
| Function | Alpha-mannosidase-like protein involved in endoplasmic reticulum-associated degradation (ERAD). Delivers misfolded glycoproteins to proteasomes. It lacks mannosidase activity. Ref.2 Ref.3 Ref.4 Ref.5 Ref.6 |
| Subcellular location | |
| Miscellaneous | Present with 656 molecules/cell in log phase SD medium. Ref.8 |
| Sequence similarities | Belongs to the glycosyl hydrolase 47 family. |
Ontologies
| Keywords | |
|---|---|
| Biological process | Unfolded protein response |
| Cellular component | Endoplasmic reticulum |
| Domain | Signal |
| PTM | Glycoprotein |
| Technical term | Complete proteome |
| Gene Ontology (GO) | |
| Biological process | ER-associated protein catabolic process Ref.2 Ref.3 Inferred from mutant phenotype. Source: SGD response to unfolded proteinInferred from electronic annotation. Source: UniProtKB-KW |
| Cellular component | endoplasmic reticulum lumen Inferred from direct assay. Source: SGD membraneInferred from electronic annotation. Source: InterPro |
| Molecular function | calcium ion binding Inferred from electronic annotation. Source: InterPro carbohydrate binding Ref.2Inferred from mutant phenotype. Source: SGD mannosyl-oligosaccharide 1,2-alpha-mannosidase activityInferred from electronic annotation. Source: InterPro protein bindingInferred from physical interaction. Source: IntAct |
| Complete GO annotation... | |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Signal peptide | 1 – 20 | 20 | Potential | ||||||
| Chain | 21 – 796 | 776 | ER degradation-enhancing alpha-mannosidase-like protein 1 | PRO_0000210324 | |||||
Amino acid modifications | |||||||||
| Glycosylation | 86 | 1 | N-linked (GlcNAc...) Potential | ||||||
| Glycosylation | 517 | 1 | N-linked (GlcNAc...) Potential | ||||||
| Glycosylation | 672 | 1 | N-linked (GlcNAc...) Potential | ||||||
| Glycosylation | 762 | 1 | N-linked (GlcNAc...) Potential | ||||||
Sequences
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References
| « Hide 'large scale' references | |
| [1] | "Complete nucleotide sequence of Saccharomyces cerevisiae chromosome VIII." Johnston M., Andrews S., Brinkman R., Cooper J., Ding H., Dover J., Du Z., Favello A., Fulton L., Gattung S., Geisel C., Kirsten J., Kucaba T., Hillier L.W., Jier M., Johnston L., Langston Y., Latreille P. Vaudin M.Science 265:2077-2082(1994) [PubMed: 8091229] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972. |
| [2] | "Htm1p, a mannosidase-like protein, is involved in glycoprotein degradation in yeast." Jakob C.A., Bodmer D., Spirig U., Baettig P., Marcil A., Dignard D., Bergeron J.J.M., Thomas D.Y., Aebi M. EMBO Rep. 2:423-430(2001) [PubMed: 11375935] [Abstract] Cited for: FUNCTION. |
| [3] | "Mnl1p, an alpha -mannosidase-like protein in yeast Saccharomyces cerevisiae, is required for endoplasmic reticulum-associated degradation of glycoproteins." Nakatsukasa K., Nishikawa S., Hosokawa N., Nagata K., Endo T. J. Biol. Chem. 276:8635-8638(2001) [PubMed: 11254655] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION. |
| [4] | "Misfolded proteins are sorted by a sequential checkpoint mechanism of ER quality control." Vashist S., Ng D.T.W. J. Cell Biol. 165:41-52(2004) [PubMed: 15078901] [Abstract] Cited for: FUNCTION. |
| [5] | "Cystic fibrosis transmembrane conductance regulator degradation depends on the lectins Htm1p/EDEM and the Cdc48 protein complex in yeast." Gnann A., Riordan J.R., Wolf D.H. Mol. Biol. Cell 15:4125-4135(2004) [PubMed: 15215312] [Abstract] Cited for: FUNCTION. |
| [6] | "Single, context-specific glycans can target misfolded glycoproteins for ER-associated degradation." Spear E.D., Ng D.T.W. J. Cell Biol. 169:73-82(2005) [PubMed: 15809311] [Abstract] Cited for: FUNCTION. |
| [7] | "Global analysis of protein localization in budding yeast." Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K. Nature 425:686-691(2003) [PubMed: 14562095] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS]. |
| [8] | "Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS]. |
| + | Additional computationally mapped references. |
Cross-references
Sequence databases | |
|---|---|
| U00030 Genomic DNA. Translation: AAB68370.1. | |
| PIR | S46693. |
| RefSeq | NP_012074.1. |
3D structure databases | |
| ModBase | Search... |
Protein-protein interaction databases | |
| DIP | DIP:1890N. |
| IntAct | P38888. 6 interactions. |
| STRING | P38888. |
Protein family/group databases | |
| CAZy | GH47. Glycoside Hydrolase Family 47. |
Genome annotation databases | |
| Ensembl | YHR204W; YHR204W; YHR204W; Saccharomyces cerevisiae. [Genome view] |
| GeneID | 856611. |
| KEGG | sce:YHR204W. |
| NMPDR | fig|4932.3.peg.3245. |
Organism-specific databases | |
| CYGD | YHR204w. |
| SGD | S000001247. MNL1. |
Phylogenomic databases | |
| HOGENOM | P38888. |
| OMA | SSHLYAT |
| OrthoDB | EOG9WM6B4 |
Gene expression databases | |
| ArrayExpress | P38888. |
| Genevestigator | P38888. |
| GermOnline | YHR204W. Saccharomyces cerevisiae. |
Family and domain databases | |
| InterPro | IPR001382. Glyco_hydro_47. [Graphical view] |
| Gene3D | G3DSA:1.50.10.50. Glyco_hydro_47. 1 hit. |
| PANTHER | PTHR11742. Glyco_hydro_47. 1 hit. |
| Pfam | PF01532. Glyco_hydro_47. 1 hit. [Graphical view] |
| PRINTS | PR00747. GLYHDRLASE47. |
| ProtoNet | Search... |
Other Resources | |
| NextBio | 982529. |
Entry information
| Entry name | MNL1_YEAST | ||||||||
| Accession | Primary (citable) accession number: P38888 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation project | FPAP (Fungal Proteome Annotation Project) | ||||||||
Relevant documents
| Glycosyl hydrolases Classification of glycosyl hydrolase families and list of entries |
| SIMILARITY comments Index of protein domains and families |
| Yeast Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD |
| Yeast chromosome VIII Yeast (Saccharomyces cerevisiae) chromosome VIII: entries and gene names |

Clusters with


