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Protein

ER degradation-enhancing alpha-mannosidase-like protein 1

Gene

MNL1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Alpha-1,2-specific exomannosidase involved in endoplasmic reticulum-associated degradation (ERAD). Delivers misfolded glycoproteins to proteasomes. Forms a complex with PDI1 to process unfolded protein-bound Man8GlcNAc2 oligosaccharides to Man7GlcNAc2, promoting degradation in unfolded protein response.7 Publications

Catalytic activityi

Hydrolysis of terminal, non-reducing alpha-D-mannose residues in alpha-D-mannosides.2 Publications

Cofactori

Ca2+By similarity

Pathwayi: protein glycosylation

This protein is involved in the pathway protein glycosylation, which is part of Protein modification.By similarity
View all proteins of this organism that are known to be involved in the pathway protein glycosylation and in Protein modification.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei372Proton donorBy similarity1
Metal bindingi495CalciumBy similarity1

GO - Molecular functioni

  • calcium ion binding Source: InterPro
  • carbohydrate binding Source: SGD
  • mannosyl-oligosaccharide 1,2-alpha-mannosidase activity Source: SGD

GO - Biological processi

  • endoplasmic reticulum mannose trimming Source: SGD
  • endoplasmic reticulum unfolded protein response Source: GO_Central
  • glycoprotein ERAD pathway Source: SGD
  • mannose trimming involved in glycoprotein ERAD pathway Source: SGD
  • N-glycan processing Source: GO_Central
  • protein alpha-1,2-demannosylation Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Keywords - Biological processi

Unfolded protein response

Keywords - Ligandi

Metal-binding

Enzyme and pathway databases

BioCyciYEAST:YHR204W-MONOMER.
ReactomeiR-SCE-901032. ER Quality Control Compartment (ERQC).
UniPathwayiUPA00378.

Protein family/group databases

CAZyiGH47. Glycoside Hydrolase Family 47.

Names & Taxonomyi

Protein namesi
Recommended name:
ER degradation-enhancing alpha-mannosidase-like protein 1 (EC:3.2.1.242 Publications)
Gene namesi
Name:MNL1
Synonyms:HTM1
Ordered Locus Names:YHR204W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome VIII

Organism-specific databases

EuPathDBiFungiDB:YHR204W.
SGDiS000001247. MNL1.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 20Sequence analysisAdd BLAST20
ChainiPRO_000021032421 – 796ER degradation-enhancing alpha-mannosidase-like protein 1Add BLAST776

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi86N-linked (GlcNAc...)Sequence analysis1
Glycosylationi517N-linked (GlcNAc...)Sequence analysis1
Glycosylationi672N-linked (GlcNAc...)Sequence analysis1
Glycosylationi762N-linked (GlcNAc...)Sequence analysis1

Keywords - PTMi

Glycoprotein

Proteomic databases

MaxQBiP38888.
PRIDEiP38888.

Interactioni

Subunit structurei

Interacts with PDI1.2 Publications

Protein-protein interaction databases

BioGridi36638. 19 interactors.
DIPiDIP-1890N.
IntActiP38888. 4 interactors.
MINTiMINT-393041.

Structurei

3D structure databases

ProteinModelPortaliP38888.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyl hydrolase 47 family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

GeneTreeiENSGT00530000063069.
HOGENOMiHOG000247966.
InParanoidiP38888.
OMAiLMLECTP.
OrthoDBiEOG092C2DAZ.

Family and domain databases

Gene3Di1.50.10.50. 1 hit.
InterProiIPR001382. Glyco_hydro_47.
[Graphical view]
PfamiPF01532. Glyco_hydro_47. 1 hit.
[Graphical view]
PRINTSiPR00747. GLYHDRLASE47.
SUPFAMiSSF48225. SSF48225. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P38888-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVCCLWVLLA LLLHLDHVAC EDDAYSFTSK ELKAYKQEVK ELFYFGFDNY
60 70 80 90 100
LEHGYPYDEV KPISCVPKKR NFEDPTDQGT NDILGNFTIT LIDSLTTIAI
110 120 130 140 150
LEDRPQFLKA VRLVERTFPD GNFDIDSTIQ VFEITIRVIG SLLSSHLYAT
160 170 180 190 200
DPTKAVYLGD DYDGSLLRLA QNMADRLLPA YLTSTGLPMP RRNIKRKWDV
210 220 230 240 250
SEFPEFLETE NNVAAMASPM FEFTILSYLT GDPKYEKVTR YAFDKTWSLR
260 270 280 290 300
TGLDLLPMSF HPEKLTPYTP MTGIGASIDS LFEYALKGAI LFDDSELMEV
310 320 330 340 350
WNVAYEALKT NCKNDWFFAN VMADTGHLFV PWIDSLSAFF SGLQVLAGDL
360 370 380 390 400
DDAIANHLMF LKMWNTFGGI PERWNFSPPE FPPLSPLERS GAVALDNILP
410 420 430 440 450
LEWYPLRPEF FESTYFLYRA TKDPFYLNIG VHLLKDLKQR FKSNCGFAGF
460 470 480 490 500
QNVITGELQD RMETFVLSET LKYLYLLFDE ENELHNSASD VIFSTEAHPM
510 520 530 540 550
WLPQEVRSNY KRNAKFNNSV YSSHLEICQK KDREQAGENT LSQRIVGFAK
560 570 580 590 600
SIFHKGPPDE EATDPIIDYT IDTELPGTCS IKPHHVIGDE FWYSPMLSNF
610 620 630 640 650
DRLFEIDSRF AATLIKPSHM HNYNAIELEP GFYNRWSNPQ FSTCLIPPTT
660 670 680 690 700
EIFELLFDLP GYHQLNPLML ENKTITFETF GGRSRLKIEK LQIYQIDYYG
710 720 730 740 750
DLITASTFQD VSRKDIFSNA CDAVASLYSP TYLYRVVAIN GRILPRHGSV
760 770 780 790
QIKKHSPVLT SNGTREEDEF KMDGIGINDH SQLMLECTPI INLFIV
Length:796
Mass (Da):91,246
Last modified:February 1, 1995 - v1
Checksum:iD538E6F28D35BE80
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00030 Genomic DNA. Translation: AAB68370.1.
BK006934 Genomic DNA. Translation: DAA06897.1.
PIRiS46693.
RefSeqiNP_012074.3. NM_001179335.3.

Genome annotation databases

EnsemblFungiiYHR204W; YHR204W; YHR204W.
GeneIDi856611.
KEGGisce:YHR204W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00030 Genomic DNA. Translation: AAB68370.1.
BK006934 Genomic DNA. Translation: DAA06897.1.
PIRiS46693.
RefSeqiNP_012074.3. NM_001179335.3.

3D structure databases

ProteinModelPortaliP38888.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi36638. 19 interactors.
DIPiDIP-1890N.
IntActiP38888. 4 interactors.
MINTiMINT-393041.

Protein family/group databases

CAZyiGH47. Glycoside Hydrolase Family 47.

Proteomic databases

MaxQBiP38888.
PRIDEiP38888.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYHR204W; YHR204W; YHR204W.
GeneIDi856611.
KEGGisce:YHR204W.

Organism-specific databases

EuPathDBiFungiDB:YHR204W.
SGDiS000001247. MNL1.

Phylogenomic databases

GeneTreeiENSGT00530000063069.
HOGENOMiHOG000247966.
InParanoidiP38888.
OMAiLMLECTP.
OrthoDBiEOG092C2DAZ.

Enzyme and pathway databases

UniPathwayiUPA00378.
BioCyciYEAST:YHR204W-MONOMER.
ReactomeiR-SCE-901032. ER Quality Control Compartment (ERQC).

Miscellaneous databases

PROiP38888.

Family and domain databases

Gene3Di1.50.10.50. 1 hit.
InterProiIPR001382. Glyco_hydro_47.
[Graphical view]
PfamiPF01532. Glyco_hydro_47. 1 hit.
[Graphical view]
PRINTSiPR00747. GLYHDRLASE47.
SUPFAMiSSF48225. SSF48225. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiMNL1_YEAST
AccessioniPrimary (citable) accession number: P38888
Secondary accession number(s): D3DLF3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: February 1, 1995
Last modified: November 2, 2016
This is version 132 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 656 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families
  4. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  5. Yeast chromosome VIII
    Yeast (Saccharomyces cerevisiae) chromosome VIII: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.