P38886 (RPN10_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
26S proteasome regulatory subunit RPN10

Gene names

Name:	RPN10
Synonyms:	MCB1, SUN1
Ordered Locus Names:	YHR200W

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	268 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Multiubiquitin binding protein.
Subunit structure	The 26S proteasome is composed of a core protease, known as the 20S proteasome, capped at one or both ends by the 19S regulatory complex (RC). The RC is composed of at least 18 different subunits in two subcomplexes, the base and the lid, which form the portions proximal and distal to the 20S proteolytic core, respectively By similarity.
Miscellaneous	Present with 17200 molecules/cell in log phase SD medium. Ref.6
Sequence similarities	Belongs to the proteasome subunit S5A family. Contains 1 UIM (ubiquitin-interacting motif) repeat. Contains 1 VWFA domain.

Ontologies

Keywords
Cellular component	Proteasome
PTM	Phosphoprotein
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological process	DNA repair Inferred from electronic annotation. Source: InterPro regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: InterPro ubiquitin-dependent protein catabolic process Inferred from mutant phenotype. Source: SGD
Cellular component	proteasome regulatory particle, base subcomplex Inferred from direct assay. Source: SGD
Molecular function	polyubiquitin binding Inferred from direct assay. Source: SGD structural molecule activity Inferred from mutant phenotype. Source: SGD zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
RPN1	P38764	5	EBI-15949,EBI-15913

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed Ref.5
	Chain	2 – 268	267	26S proteasome regulatory subunit RPN10		PRO_0000173834
Regions
	Domain	5 – 190	186	VWFA
	Repeat	223 – 242	20	UIM
	Compositional bias	248 – 255	8	Poly-Gln
Amino acid modifications
	Modified residue	259	1	Phosphoserine Ref.7

Sequences

	Sequence		Length	Mass (Da)
	P38886 [UniParc]. Last modified January 23, 2007. Version 3. Checksum: 8A05198B0CD7C0C3	FASTA	268	29,747
	10 20 30 40 50 60 MVLEATVLVI DNSEYSRNGD FPRTRFEAQI DSVEFIFQAK RNSNPENTVG LISGAGANPR 70 80 90 100 110 120 VLSTFTAEFG KILAGLHDTQ IEGKLHMATA LQIAQLTLKH RQNKVQHQRI VAFVCSPISD 130 140 150 160 170 180 SRDELIRLAK TLKKNNVAVD IINFGEIEQN TELLDEFIAA VNNPQEETSH LLTVTPGPRL 190 200 210 220 230 240 LYENIASSPI ILEEGSSGMG AFGGSGGDSD ANGTFMDFGV DPSMDPELAM ALRLSMEEEQ 250 260 QRQERLRQQQ QQQDQPEQSE QPEQHQDK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Yeast counterparts of subunits S5a and p58 (S3) of the human 26S proteasome are encoded by two multicopy suppressors of nin1-1." Kominami K., Okura N., Kawamura M., Demartino G.N., Slaughter C.A., Shimbara N., Chung C.H., Fujimuro M., Yokosawa H., Shimizu Y., Tanahashi N., Tanaka K., Toh-e A. Mol. Biol. Cell 8:171-187(1997) [PubMed: 9017604] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Complete nucleotide sequence of Saccharomyces cerevisiae chromosome VIII." Johnston M., Andrews S., Brinkman R., Cooper J., Ding H., Dover J., Du Z., Favello A., Fulton L., Gattung S., Geisel C., Kirsten J., Kucaba T., Hillier L.W., Jier M., Johnston L., Langston Y., Latreille P. , Louis E.J., Macri C., Mardis E., Menezes S., Mouser L., Nhan M., Rifkin L., Riles L., St Peter H., Trevaskis E., Vaughan K., Vignati D., Wilcox L., Wohldman P., Waterston R., Wilson R., Vaudin M. Science 265:2077-2082(1994) [PubMed: 8091229] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[3]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[4]	"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae." Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J. Genome Res. 17:536-543(2007) [PubMed: 17322287] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[5]	"N-terminal modifications of the 19S regulatory particle subunits of the yeast proteasome." Kimura Y., Saeki Y., Yokosawa H., Polevoda B., Sherman F., Hirano H. Arch. Biochem. Biophys. 409:341-348(2003) [PubMed: 12504901] [Abstract] Cited for: PROTEIN SEQUENCE OF 2-8.
[6]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[7]	"Proteome-wide identification of in vivo targets of DNA damage checkpoint kinases." Smolka M.B., Albuquerque C.P., Chen S.H., Zhou H. Proc. Natl. Acad. Sci. U.S.A. 104:10364-10369(2007) [PubMed: 17563356] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-259, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	D78022 Genomic DNA. Translation: BAA11207.1. U00030 Genomic DNA. Translation: AAB68355.1. AY558289 Genomic DNA. Translation: AAS56615.1. BK006934 Genomic DNA. Translation: DAA06893.1.
PIR	S46677.
RefSeq	NP_012070.1. NM_001179331.1.
3D structure databases
ProteinModelPortal	P38886.
SMR	P38886. Positions 211-259.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-2100N.
IntAct	P38886. 49 interactions.
MINT	MINT-557564.
STRING	P38886.
Proteomic databases
PeptideAtlas	P38886.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YHR200W; YHR200W; YHR200W.
GeneID	856607.
KEGG	sce:YHR200W.
NMPDR	fig|4932.3.peg.3241.
Organism-specific databases
SGD	S000001243. RPN10.
Phylogenomic databases
eggNOG	fuNOG05401.
GeneTree	EFGT00050000005719.
HOGENOM	HBG738415.
OMA	VEFIFQA.
OrthoDB	EOG4GXJX7.
Gene expression databases
ArrayExpress	P38886.
Genevestigator	P38886.
GermOnline	YHR200W. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR007198. Ssl1-like. IPR003903. Ubiquitin-int_motif. IPR002035. VWF_A. [Graphical view]
KO	K03029.
Pfam	PF04056. Ssl1. 1 hit. [Graphical view]
SMART	SM00726. UIM. 1 hit. SM00327. VWA. 1 hit. [Graphical view]
PROSITE	PS50330. UIM. 1 hit. PS50234. VWFA. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	982517.

Entry information

Entry name

RPN10_YEAST

Accession

Primary (citable) accession number: P38886
Secondary accession number(s): D3DLE9

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1995
Last sequence update:	January 23, 2007
Last modified:	December 14, 2011
This is version 98 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program