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Protein

26S proteasome regulatory subunit RPN10

Gene

RPN10

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Multiubiquitin binding protein.

Miscellaneous

Present with 17200 molecules/cell in log phase SD medium.1 Publication

GO - Molecular functioni

  • polyubiquitin modification-dependent protein binding Source: SGD
  • structural molecule activity Source: SGD

GO - Biological processi

Enzyme and pathway databases

BioCyciYEAST:G3O-31228-MONOMER
ReactomeiR-SCE-1236978 Cross-presentation of soluble exogenous antigens (endosomes)
R-SCE-174113 SCF-beta-TrCP mediated degradation of Emi1
R-SCE-187577 SCF(Skp2)-mediated degradation of p27/p21
R-SCE-349425 Autodegradation of the E3 ubiquitin ligase COP1
R-SCE-382556 ABC-family proteins mediated transport
R-SCE-450408 AUF1 (hnRNP D0) binds and destabilizes mRNA
R-SCE-5668541 TNFR2 non-canonical NF-kB pathway
R-SCE-5687128 MAPK6/MAPK4 signaling
R-SCE-5689880 Ub-specific processing proteases
R-SCE-68949 Orc1 removal from chromatin
R-SCE-69017 CDK-mediated phosphorylation and removal of Cdc6
R-SCE-69601 Ubiquitin Mediated Degradation of Phosphorylated Cdc25A
R-SCE-8854050 FBXL7 down-regulates AURKA during mitotic entry and in early mitosis
R-SCE-983168 Antigen processing: Ubiquitination & Proteasome degradation

Names & Taxonomyi

Protein namesi
Recommended name:
26S proteasome regulatory subunit RPN10
Gene namesi
Name:RPN10
Synonyms:MCB1, SUN1
Ordered Locus Names:YHR200W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome VIII

Organism-specific databases

EuPathDBiFungiDB:YHR200W
SGDiS000001243 RPN10

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Proteasome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved1 Publication
ChainiPRO_00001738342 – 26826S proteasome regulatory subunit RPN10Add BLAST267

Proteomic databases

MaxQBiP38886
PaxDbiP38886
PRIDEiP38886

PTM databases

iPTMnetiP38886

Interactioni

Subunit structurei

The 26S proteasome is composed of a core protease, known as the 20S proteasome, capped at one or both ends by the 19S regulatory complex (RC). The RC is composed of at least 18 different subunits in two subcomplexes, the base and the lid, which form the portions proximal and distal to the 20S proteolytic core, respectively (By similarity).By similarity

Binary interactionsi

Show more details

GO - Molecular functioni

  • polyubiquitin modification-dependent protein binding Source: SGD

Protein-protein interaction databases

BioGridi36634, 815 interactors
DIPiDIP-2100N
IntActiP38886, 59 interactors
MINTiP38886
STRINGi4932.YHR200W

Structurei

Secondary structure

1268
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi4 – 10Combined sources7
Helixi14 – 16Combined sources3
Beta strandi19 – 23Combined sources5
Helixi25 – 43Combined sources19
Beta strandi48 – 53Combined sources6
Beta strandi55 – 58Combined sources4
Beta strandi60 – 67Combined sources8
Helixi69 – 76Combined sources8
Helixi87 – 99Combined sources13
Beta strandi106 – 114Combined sources9
Helixi122 – 134Combined sources13
Beta strandi137 – 143Combined sources7
Helixi154 – 161Combined sources8
Beta strandi164 – 166Combined sources3
Beta strandi171 – 173Combined sources3
Beta strandi176 – 179Combined sources4
Helixi181 – 187Combined sources7

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3JCOelectron microscopy4.80W1-268[»]
3JCPelectron microscopy4.60W1-268[»]
4CR2electron microscopy7.70W1-268[»]
4CR3electron microscopy9.30W1-268[»]
4CR4electron microscopy8.80W1-268[»]
5A5Belectron microscopy9.50W1-268[»]
5LN1X-ray3.14A1-191[»]
5MPBelectron microscopy7.80W1-268[»]
5MPCelectron microscopy7.70W1-268[»]
5MPDelectron microscopy4.10W1-268[»]
5MPEelectron microscopy4.50W1-268[»]
5WVIelectron microscopy6.30W1-268[»]
5WVKelectron microscopy4.20W1-268[»]
ProteinModelPortaliP38886
SMRiP38886
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini5 – 190VWFAPROSITE-ProRule annotationAdd BLAST186
Domaini223 – 242UIMPROSITE-ProRule annotationAdd BLAST20

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi248 – 255Poly-Gln8

Sequence similaritiesi

Belongs to the proteasome subunit S5A family.Curated

Phylogenomic databases

GeneTreeiENSGT00530000064050
HOGENOMiHOG000165630
InParanoidiP38886
KOiK03029
OMAiMGGKGPE
OrthoDBiEOG092C5246

Family and domain databases

Gene3Di3.40.50.410, 1 hit
InterProiView protein in InterPro
IPR027040 Proteasome_su_Rpn10
IPR003903 UIM_dom
IPR002035 VWF_A
IPR036465 vWFA_dom_sf
PANTHERiPTHR10223:SF0 PTHR10223:SF0, 1 hit
PfamiView protein in Pfam
PF13519 VWA_2, 1 hit
SUPFAMiSSF53300 SSF53300, 1 hit
PROSITEiView protein in PROSITE
PS50330 UIM, 1 hit
PS50234 VWFA, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P38886-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVLEATVLVI DNSEYSRNGD FPRTRFEAQI DSVEFIFQAK RNSNPENTVG
60 70 80 90 100
LISGAGANPR VLSTFTAEFG KILAGLHDTQ IEGKLHMATA LQIAQLTLKH
110 120 130 140 150
RQNKVQHQRI VAFVCSPISD SRDELIRLAK TLKKNNVAVD IINFGEIEQN
160 170 180 190 200
TELLDEFIAA VNNPQEETSH LLTVTPGPRL LYENIASSPI ILEEGSSGMG
210 220 230 240 250
AFGGSGGDSD ANGTFMDFGV DPSMDPELAM ALRLSMEEEQ QRQERLRQQQ
260
QQQDQPEQSE QPEQHQDK
Length:268
Mass (Da):29,747
Last modified:January 23, 2007 - v3
Checksum:i8A05198B0CD7C0C3
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D78022 Genomic DNA Translation: BAA11207.1
U00030 Genomic DNA Translation: AAB68355.1
AY558289 Genomic DNA Translation: AAS56615.1
BK006934 Genomic DNA Translation: DAA06893.1
PIRiS46677
RefSeqiNP_012070.3, NM_001179331.3

Genome annotation databases

EnsemblFungiiYHR200W; YHR200W; YHR200W
GeneIDi856607
KEGGisce:YHR200W

Similar proteinsi

Entry informationi

Entry nameiRPN10_YEAST
AccessioniPrimary (citable) accession number: P38886
Secondary accession number(s): D3DLE9
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: January 23, 2007
Last modified: May 23, 2018
This is version 163 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

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