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Protein

26S proteasome regulatory subunit RPN10

Gene

RPN10

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Multiubiquitin binding protein.

GO - Molecular functioni

  • polyubiquitin binding Source: SGD
  • structural molecule activity Source: SGD

GO - Biological processi

  • ubiquitin-dependent protein catabolic process Source: SGD
Complete GO annotation...

Enzyme and pathway databases

BioCyciYEAST:G3O-31228-MONOMER.
ReactomeiR-SCE-1236978. Cross-presentation of soluble exogenous antigens (endosomes).
R-SCE-174113. SCF-beta-TrCP mediated degradation of Emi1.
R-SCE-349425. Autodegradation of the E3 ubiquitin ligase COP1.
R-SCE-450408. AUF1 (hnRNP D0) binds and destabilizes mRNA.
R-SCE-5668541. TNFR2 non-canonical NF-kB pathway.
R-SCE-5687128. MAPK6/MAPK4 signaling.
R-SCE-68949. Orc1 removal from chromatin.
R-SCE-69017. CDK-mediated phosphorylation and removal of Cdc6.
R-SCE-69229. Ubiquitin-dependent degradation of Cyclin D1.
R-SCE-69601. Ubiquitin Mediated Degradation of Phosphorylated Cdc25A.
R-SCE-983168. Antigen processing: Ubiquitination & Proteasome degradation.

Names & Taxonomyi

Protein namesi
Recommended name:
26S proteasome regulatory subunit RPN10
Gene namesi
Name:RPN10
Synonyms:MCB1, SUN1
Ordered Locus Names:YHR200W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome VIII

Organism-specific databases

EuPathDBiFungiDB:YHR200W.
SGDiS000001243. RPN10.

Subcellular locationi

GO - Cellular componenti

  • proteasome complex Source: SGD
  • proteasome regulatory particle, base subcomplex Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Proteasome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemoved1 Publication
Chaini2 – 26826726S proteasome regulatory subunit RPN10PRO_0000173834Add
BLAST

Proteomic databases

MaxQBiP38886.
PeptideAtlasiP38886.

PTM databases

iPTMnetiP38886.

Interactioni

Subunit structurei

The 26S proteasome is composed of a core protease, known as the 20S proteasome, capped at one or both ends by the 19S regulatory complex (RC). The RC is composed of at least 18 different subunits in two subcomplexes, the base and the lid, which form the portions proximal and distal to the 20S proteolytic core, respectively (By similarity).By similarity

Binary interactionsi

WithEntry#Exp.IntActNotes
DSK2P485107EBI-15949,EBI-6174
RPN1P387646EBI-15949,EBI-15913
RPT5P332972EBI-15949,EBI-13920

GO - Molecular functioni

  • polyubiquitin binding Source: SGD

Protein-protein interaction databases

BioGridi36634. 665 interactions.
DIPiDIP-2100N.
IntActiP38886. 55 interactions.
MINTiMINT-557564.

Structurei

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4CR2electron microscopy7.70W1-268[»]
4CR3electron microscopy9.30W1-268[»]
4CR4electron microscopy8.80W1-268[»]
5A5Belectron microscopy9.50W1-268[»]
ProteinModelPortaliP38886.
SMRiP38886. Positions 1-197.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini5 – 190186VWFAPROSITE-ProRule annotationAdd
BLAST
Domaini223 – 24220UIMPROSITE-ProRule annotationAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi248 – 2558Poly-Gln

Sequence similaritiesi

Belongs to the proteasome subunit S5A family.Curated
Contains 1 UIM (ubiquitin-interacting motif) domain.PROSITE-ProRule annotation
Contains 1 VWFA domain.PROSITE-ProRule annotation

Phylogenomic databases

GeneTreeiENSGT00530000064050.
HOGENOMiHOG000165630.
InParanoidiP38886.
KOiK03029.
OMAiATSPILM.
OrthoDBiEOG7VDZ15.

Family and domain databases

Gene3Di3.40.50.410. 1 hit.
InterProiIPR027040. Proteasome_su_Rpn10.
IPR003903. UIM_dom.
IPR002035. VWF_A.
[Graphical view]
PANTHERiPTHR10223. PTHR10223. 1 hit.
PfamiPF13519. VWA_2. 1 hit.
[Graphical view]
SUPFAMiSSF53300. SSF53300. 1 hit.
PROSITEiPS50330. UIM. 1 hit.
PS50234. VWFA. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P38886-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVLEATVLVI DNSEYSRNGD FPRTRFEAQI DSVEFIFQAK RNSNPENTVG
60 70 80 90 100
LISGAGANPR VLSTFTAEFG KILAGLHDTQ IEGKLHMATA LQIAQLTLKH
110 120 130 140 150
RQNKVQHQRI VAFVCSPISD SRDELIRLAK TLKKNNVAVD IINFGEIEQN
160 170 180 190 200
TELLDEFIAA VNNPQEETSH LLTVTPGPRL LYENIASSPI ILEEGSSGMG
210 220 230 240 250
AFGGSGGDSD ANGTFMDFGV DPSMDPELAM ALRLSMEEEQ QRQERLRQQQ
260
QQQDQPEQSE QPEQHQDK
Length:268
Mass (Da):29,747
Last modified:January 23, 2007 - v3
Checksum:i8A05198B0CD7C0C3
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D78022 Genomic DNA. Translation: BAA11207.1.
U00030 Genomic DNA. Translation: AAB68355.1.
AY558289 Genomic DNA. Translation: AAS56615.1.
BK006934 Genomic DNA. Translation: DAA06893.1.
PIRiS46677.
RefSeqiNP_012070.3. NM_001179331.3.

Genome annotation databases

EnsemblFungiiYHR200W; YHR200W; YHR200W.
GeneIDi856607.
KEGGisce:YHR200W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D78022 Genomic DNA. Translation: BAA11207.1.
U00030 Genomic DNA. Translation: AAB68355.1.
AY558289 Genomic DNA. Translation: AAS56615.1.
BK006934 Genomic DNA. Translation: DAA06893.1.
PIRiS46677.
RefSeqiNP_012070.3. NM_001179331.3.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4CR2electron microscopy7.70W1-268[»]
4CR3electron microscopy9.30W1-268[»]
4CR4electron microscopy8.80W1-268[»]
5A5Belectron microscopy9.50W1-268[»]
ProteinModelPortaliP38886.
SMRiP38886. Positions 1-197.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi36634. 665 interactions.
DIPiDIP-2100N.
IntActiP38886. 55 interactions.
MINTiMINT-557564.

PTM databases

iPTMnetiP38886.

Proteomic databases

MaxQBiP38886.
PeptideAtlasiP38886.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYHR200W; YHR200W; YHR200W.
GeneIDi856607.
KEGGisce:YHR200W.

Organism-specific databases

EuPathDBiFungiDB:YHR200W.
SGDiS000001243. RPN10.

Phylogenomic databases

GeneTreeiENSGT00530000064050.
HOGENOMiHOG000165630.
InParanoidiP38886.
KOiK03029.
OMAiATSPILM.
OrthoDBiEOG7VDZ15.

Enzyme and pathway databases

BioCyciYEAST:G3O-31228-MONOMER.
ReactomeiR-SCE-1236978. Cross-presentation of soluble exogenous antigens (endosomes).
R-SCE-174113. SCF-beta-TrCP mediated degradation of Emi1.
R-SCE-349425. Autodegradation of the E3 ubiquitin ligase COP1.
R-SCE-450408. AUF1 (hnRNP D0) binds and destabilizes mRNA.
R-SCE-5668541. TNFR2 non-canonical NF-kB pathway.
R-SCE-5687128. MAPK6/MAPK4 signaling.
R-SCE-68949. Orc1 removal from chromatin.
R-SCE-69017. CDK-mediated phosphorylation and removal of Cdc6.
R-SCE-69229. Ubiquitin-dependent degradation of Cyclin D1.
R-SCE-69601. Ubiquitin Mediated Degradation of Phosphorylated Cdc25A.
R-SCE-983168. Antigen processing: Ubiquitination & Proteasome degradation.

Miscellaneous databases

PROiP38886.

Family and domain databases

Gene3Di3.40.50.410. 1 hit.
InterProiIPR027040. Proteasome_su_Rpn10.
IPR003903. UIM_dom.
IPR002035. VWF_A.
[Graphical view]
PANTHERiPTHR10223. PTHR10223. 1 hit.
PfamiPF13519. VWA_2. 1 hit.
[Graphical view]
SUPFAMiSSF53300. SSF53300. 1 hit.
PROSITEiPS50330. UIM. 1 hit.
PS50234. VWFA. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Yeast counterparts of subunits S5a and p58 (S3) of the human 26S proteasome are encoded by two multicopy suppressors of nin1-1."
    Kominami K., Okura N., Kawamura M., Demartino G.N., Slaughter C.A., Shimbara N., Chung C.H., Fujimuro M., Yokosawa H., Shimizu Y., Tanahashi N., Tanaka K., Toh-e A.
    Mol. Biol. Cell 8:171-187(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  4. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  5. "N-terminal modifications of the 19S regulatory particle subunits of the yeast proteasome."
    Kimura Y., Saeki Y., Yokosawa H., Polevoda B., Sherman F., Hirano H.
    Arch. Biochem. Biophys. 409:341-348(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 2-8.
  6. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  7. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  8. Cited for: STRUCTURE BY ELECTRON MICROSCOPY (7.4 ANGSTROMS) OF THE 26S PROTEASOME.

Entry informationi

Entry nameiRPN10_YEAST
AccessioniPrimary (citable) accession number: P38886
Secondary accession number(s): D3DLE9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: January 23, 2007
Last modified: June 8, 2016
This is version 143 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 17200 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome VIII
    Yeast (Saccharomyces cerevisiae) chromosome VIII: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.