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P38883

- RIX1_YEAST

UniProt

P38883 - RIX1_YEAST

Protein

Pre-rRNA-processing protein RIX1

Gene

RIX1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 103 (01 Oct 2014)
      Sequence version 2 (27 Jul 2011)
      Previous versions | rss
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    Functioni

    Component of the RIX1 complex required for processing of ITS2 sequences from 35S pre-rRNA.3 Publications

    GO - Molecular functioni

    1. chromatin binding Source: SGD
    2. protein binding Source: IntAct

    GO - Biological processi

    1. pre-replicative complex assembly involved in nuclear cell cycle DNA replication Source: SGD
    2. regulation of DNA-dependent DNA replication initiation Source: SGD
    3. ribosomal large subunit assembly Source: SGD
    4. ribosomal subunit export from nucleus Source: SGD
    5. rRNA processing Source: SGD

    Keywords - Biological processi

    Ribosome biogenesis, rRNA processing

    Enzyme and pathway databases

    BioCyciYEAST:G3O-31225-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Pre-rRNA-processing protein RIX1
    Alternative name(s):
    Involved in processing IST2 protein 2
    Ribosomal export protein 1
    Gene namesi
    Name:RIX1
    Synonyms:IPI2
    Ordered Locus Names:YHR197W
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome VIII

    Organism-specific databases

    CYGDiYHR197w.
    SGDiS000001240. RIX1.

    Subcellular locationi

    Nucleus 3 Publications

    GO - Cellular componenti

    1. cytosol Source: SGD
    2. nucleoplasm Source: SGD
    3. nucleus Source: SGD
    4. Rix1 complex Source: SGD

    Keywords - Cellular componenti

    Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11Removed1 Publication
    Chaini2 – 763762Pre-rRNA-processing protein RIX1PRO_0000202938Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei2 – 21N-acetylserine1 Publication

    Keywords - PTMi

    Acetylation

    Proteomic databases

    MaxQBiP38883.
    PaxDbiP38883.
    PeptideAtlasiP38883.

    Expressioni

    Gene expression databases

    GenevestigatoriP38883.

    Interactioni

    Subunit structurei

    Component of the RIX1 complex, composed of at least IPI1, IPI3 and RIX1. The complex interacts with MDN1 and the pre-60S ribosome particles. Interacts with NUG1.5 Publications

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    IPI3P538777EBI-24899,EBI-29063

    Protein-protein interaction databases

    BioGridi36631. 97 interactions.
    DIPiDIP-804N.
    IntActiP38883. 45 interactions.
    MINTiMINT-403308.
    STRINGi4932.YHR197W.

    Family & Domainsi

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi690 – 76374Glu-richAdd
    BLAST

    Sequence similaritiesi

    Belongs to the RIX1 family.Curated

    Phylogenomic databases

    eggNOGiNOG45551.
    HOGENOMiHOG000154134.
    KOiK14828.
    OMAiWCGINLI.
    OrthoDBiEOG7MKWG4.

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P38883-1 [UniParc]FASTAAdd to Basket

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    MSEEFIAVST LARNLEIAKG NEFHTILATL RSPVYINEQL LKSELSFLVT    50
    KILKLIRSGN DFDLWKGCHT SVVTCAYNPL VLSTHGGQLL AAIYSRLEQK 100
    TGFYSSVISS SHGKQLFNTL ISSVAIIIDL MKNKPTLSRE ALVPKLKAII 150
    PTLITLSQYE PELVLPVLQR ILKRNTTTFK PFTNKFRTVL INLIISDYAS 200
    LGTKTQRLVC ENFAYLHLLK IQVSDTSDDE TQAHHKIYAD SNWRTGLMSI 250
    LSQFKPIIQL CGEILDFEQD NELYKLIKSL PVIDESNNKE EFLPSLKLDF 300
    NAPLTLWEIP QRLSLLADML VAFISLPTPF PIRVPLGGIN SLCEVLLGVS 350
    NKYLPLKKEL RHDNELNGVI NTILPQIQFQ GIRLWEIMVS KYGKCGLSFF 400
    EGILSSIELF IPLKKKSNNE IDFNVVGSLK FEFATVFRLV NMILSHLGHQ 450
    LNIISVISQL IEVALFLSHD KTLIDSLFKN RKSIMKQQTK TKQSKRSKSA 500
    EGAFSDIYTH PELFVCKNSM NWFNEINDFF ITALNNWILP STPHIQILKY 550
    SITQSLRLKE RFGYIPESFV NLLRCEVLHP GSERVSILPI AISLLKNIND 600
    DMFELLCHPK VPVGMVYQLH KPLDLGEDGE VRDDINKKEV ETNESSSNAN 650
    TGLETLKALE NLENVTIPEP KHEVPKVVDD TAIFKKRSVE EVIERESTSS 700
    HKKVKFVEET TVDNGEELIV KKAVSQTKEE EKPMEDSEDE EQEEFEIPAI 750
    ELSDDEEEEE EEE 763
    Length:763
    Mass (Da):86,739
    Last modified:July 27, 2011 - v2
    Checksum:i14A0CE9A21F11711
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti762 – 7621E → G in AAB68356. (PubMed:8091229)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U00030 Genomic DNA. Translation: AAB68356.1.
    BK006934 Genomic DNA. Translation: DAA06889.2.
    PIRiS46678.
    RefSeqiNP_012067.4. NM_001179328.4.

    Genome annotation databases

    EnsemblFungiiYHR197W; YHR197W; YHR197W.
    GeneIDi856604.
    KEGGisce:YHR197W.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U00030 Genomic DNA. Translation: AAB68356.1 .
    BK006934 Genomic DNA. Translation: DAA06889.2 .
    PIRi S46678.
    RefSeqi NP_012067.4. NM_001179328.4.

    3D structure databases

    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 36631. 97 interactions.
    DIPi DIP-804N.
    IntActi P38883. 45 interactions.
    MINTi MINT-403308.
    STRINGi 4932.YHR197W.

    Proteomic databases

    MaxQBi P38883.
    PaxDbi P38883.
    PeptideAtlasi P38883.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YHR197W ; YHR197W ; YHR197W .
    GeneIDi 856604.
    KEGGi sce:YHR197W.

    Organism-specific databases

    CYGDi YHR197w.
    SGDi S000001240. RIX1.

    Phylogenomic databases

    eggNOGi NOG45551.
    HOGENOMi HOG000154134.
    KOi K14828.
    OMAi WCGINLI.
    OrthoDBi EOG7MKWG4.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-31225-MONOMER.

    Miscellaneous databases

    NextBioi 982508.

    Gene expression databases

    Genevestigatori P38883.

    Family and domain databases

    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    2. Cited for: GENOME REANNOTATION, SEQUENCE REVISION TO 762.
      Strain: ATCC 204508 / S288c.
    3. "Identification of a 60S preribosomal particle that is closely linked to nuclear export."
      Bassler J., Grandi P., Gadal O., Lessmann T., Petfalski E., Tollervey D., Lechner J., Hurt E.
      Mol. Cell 8:517-529(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH NUG1.
    4. "Nuclear export of 60s ribosomal subunits depends on Xpo1p and requires a nuclear export sequence-containing factor, Nmd3p, that associates with the large subunit protein Rpl10p."
      Gadal O., Strauss D., Kessl J., Trumpower B., Tollervey D., Hurt E.
      Mol. Cell. Biol. 21:3405-3415(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    5. Cited for: IDENTIFICATION IN THE RIX1 COMPLEX, SUBCELLULAR LOCATION, IDENTIFICATION BY MASS SPECTROMETRY.
    6. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
    7. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
    8. "Rea1, a dynein-related nuclear AAA-ATPase, is involved in late rRNA processing and nuclear export of 60 S subunits."
      Galani K., Nissan T.A., Petfalski E., Tollervey D., Hurt E.
      J. Biol. Chem. 279:55411-55418(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION IN THE RIX1 COMPLEX, SUBCELLULAR LOCATION, FUNCTION OF THE RIX1 COMPLEX, IDENTIFICATION BY MASS SPECTROMETRY.
    9. "A pre-ribosome with a tadpole-like structure functions in ATP-dependent maturation of 60S subunits."
      Nissan T.A., Galani K., Maco B., Tollervey D., Aebi U., Hurt E.
      Mol. Cell 15:295-301(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION IN THE RIX1 COMPLEX, INTERACTION WITH MDN1 AND PRE-60S RIBOSOMAL PARTICLES, IDENTIFICATION BY MASS SPECTROMETRY.
    10. Cited for: IDENTIFICATION IN THE RIX1 COMPLEX, FUNCTION OF THE RIX1 COMPLEX.
    11. Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT SER-2, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS], CLEAVAGE OF INITIATOR METHIONINE [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiRIX1_YEAST
    AccessioniPrimary (citable) accession number: P38883
    Secondary accession number(s): D3DLE5
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 1, 1995
    Last sequence update: July 27, 2011
    Last modified: October 1, 2014
    This is version 103 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 7820 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families
    2. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    3. Yeast chromosome VIII
      Yeast (Saccharomyces cerevisiae) chromosome VIII: entries and gene names

    External Data

    Dasty 3