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P38883 (RIX1_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 100. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Pre-rRNA-processing protein RIX1
Alternative name(s):
Involved in processing IST2 protein 2
Ribosomal export protein 1
Gene names
Name:RIX1
Synonyms:IPI2
Ordered Locus Names:YHR197W
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length763 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Component of the RIX1 complex required for processing of ITS2 sequences from 35S pre-rRNA. Ref.4 Ref.8 Ref.10

Subunit structure

Component of the RIX1 complex, composed of at least IPI1, IPI3 and RIX1. The complex interacts with MDN1 and the pre-60S ribosome particles. Interacts with NUG1. Ref.3 Ref.5 Ref.8 Ref.9 Ref.10

Subcellular location

Nucleus Ref.5 Ref.6 Ref.8.

Miscellaneous

Present with 7820 molecules/cell in log phase SD medium.

Sequence similarities

Belongs to the RIX1 family.

Binary interactions

With

Entry

#Exp.

IntAct

Notes

IPI3P538777EBI-24899,EBI-29063

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed Ref.11
Chain2 – 763762Pre-rRNA-processing protein RIX1
PRO_0000202938

Regions

Compositional bias690 – 76374Glu-rich

Amino acid modifications

Modified residue21N-acetylserine Ref.11

Experimental info

Sequence conflict7621E → G in AAB68356. Ref.1

Sequences

Sequence LengthMass (Da)Tools
P38883 [UniParc].

Last modified July 27, 2011. Version 2.
Checksum: 14A0CE9A21F11711

FASTA76386,739
        10         20         30         40         50         60 
MSEEFIAVST LARNLEIAKG NEFHTILATL RSPVYINEQL LKSELSFLVT KILKLIRSGN 

        70         80         90        100        110        120 
DFDLWKGCHT SVVTCAYNPL VLSTHGGQLL AAIYSRLEQK TGFYSSVISS SHGKQLFNTL 

       130        140        150        160        170        180 
ISSVAIIIDL MKNKPTLSRE ALVPKLKAII PTLITLSQYE PELVLPVLQR ILKRNTTTFK 

       190        200        210        220        230        240 
PFTNKFRTVL INLIISDYAS LGTKTQRLVC ENFAYLHLLK IQVSDTSDDE TQAHHKIYAD 

       250        260        270        280        290        300 
SNWRTGLMSI LSQFKPIIQL CGEILDFEQD NELYKLIKSL PVIDESNNKE EFLPSLKLDF 

       310        320        330        340        350        360 
NAPLTLWEIP QRLSLLADML VAFISLPTPF PIRVPLGGIN SLCEVLLGVS NKYLPLKKEL 

       370        380        390        400        410        420 
RHDNELNGVI NTILPQIQFQ GIRLWEIMVS KYGKCGLSFF EGILSSIELF IPLKKKSNNE 

       430        440        450        460        470        480 
IDFNVVGSLK FEFATVFRLV NMILSHLGHQ LNIISVISQL IEVALFLSHD KTLIDSLFKN 

       490        500        510        520        530        540 
RKSIMKQQTK TKQSKRSKSA EGAFSDIYTH PELFVCKNSM NWFNEINDFF ITALNNWILP 

       550        560        570        580        590        600 
STPHIQILKY SITQSLRLKE RFGYIPESFV NLLRCEVLHP GSERVSILPI AISLLKNIND 

       610        620        630        640        650        660 
DMFELLCHPK VPVGMVYQLH KPLDLGEDGE VRDDINKKEV ETNESSSNAN TGLETLKALE 

       670        680        690        700        710        720 
NLENVTIPEP KHEVPKVVDD TAIFKKRSVE EVIERESTSS HKKVKFVEET TVDNGEELIV 

       730        740        750        760 
KKAVSQTKEE EKPMEDSEDE EQEEFEIPAI ELSDDEEEEE EEE 

« Hide

References

« Hide 'large scale' references
[1]"Complete nucleotide sequence of Saccharomyces cerevisiae chromosome VIII."
Johnston M., Andrews S., Brinkman R., Cooper J., Ding H., Dover J., Du Z., Favello A., Fulton L., Gattung S., Geisel C., Kirsten J., Kucaba T., Hillier L.W., Jier M., Johnston L., Langston Y., Latreille P. expand/collapse author list , Louis E.J., Macri C., Mardis E., Menezes S., Mouser L., Nhan M., Rifkin L., Riles L., St Peter H., Trevaskis E., Vaughan K., Vignati D., Wilcox L., Wohldman P., Waterston R., Wilson R., Vaudin M.
Science 265:2077-2082(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[2]Saccharomyces Genome Database
Submitted (MAR-2011) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION, SEQUENCE REVISION TO 762.
Strain: ATCC 204508 / S288c.
[3]"Identification of a 60S preribosomal particle that is closely linked to nuclear export."
Bassler J., Grandi P., Gadal O., Lessmann T., Petfalski E., Tollervey D., Lechner J., Hurt E.
Mol. Cell 8:517-529(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH NUG1.
[4]"Nuclear export of 60s ribosomal subunits depends on Xpo1p and requires a nuclear export sequence-containing factor, Nmd3p, that associates with the large subunit protein Rpl10p."
Gadal O., Strauss D., Kessl J., Trumpower B., Tollervey D., Hurt E.
Mol. Cell. Biol. 21:3405-3415(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[5]"Assigning function to yeast proteins by integration of technologies."
Hazbun T.R., Malmstroem L., Anderson S., Graczyk B.J., Fox B., Riffle M., Sundin B.A., Aranda J.D., McDonald W.H., Chiu C.-H., Snydsman B.E., Bradley P., Muller E.G.D., Fields S., Baker D., Yates J.R. III, Davis T.N.
Mol. Cell 12:1353-1365(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION IN THE RIX1 COMPLEX, SUBCELLULAR LOCATION, IDENTIFICATION BY MASS SPECTROMETRY.
[6]"Global analysis of protein localization in budding yeast."
Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K.
Nature 425:686-691(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[7]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[8]"Rea1, a dynein-related nuclear AAA-ATPase, is involved in late rRNA processing and nuclear export of 60 S subunits."
Galani K., Nissan T.A., Petfalski E., Tollervey D., Hurt E.
J. Biol. Chem. 279:55411-55418(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION IN THE RIX1 COMPLEX, SUBCELLULAR LOCATION, FUNCTION OF THE RIX1 COMPLEX, IDENTIFICATION BY MASS SPECTROMETRY.
[9]"A pre-ribosome with a tadpole-like structure functions in ATP-dependent maturation of 60S subunits."
Nissan T.A., Galani K., Maco B., Tollervey D., Aebi U., Hurt E.
Mol. Cell 15:295-301(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION IN THE RIX1 COMPLEX, INTERACTION WITH MDN1 AND PRE-60S RIBOSOMAL PARTICLES, IDENTIFICATION BY MASS SPECTROMETRY.
[10]"High-definition macromolecular composition of yeast RNA-processing complexes."
Krogan N.J., Peng W.-T., Cagney G., Robinson M.D., Haw R., Zhong G., Guo X., Zhang X., Canadien V., Richards D.P., Beattie B.K., Lalev A., Zhang W., Davierwala A.P., Mnaimneh S., Starostine A., Tikuisis A.P., Grigull J. expand/collapse author list , Datta N., Bray J.E., Hughes T.R., Emili A., Greenblatt J.F.
Mol. Cell 13:225-239(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION IN THE RIX1 COMPLEX, FUNCTION OF THE RIX1 COMPLEX.
[11]"N-terminal acetylome analyses and functional insights of the N-terminal acetyltransferase NatB."
Van Damme P., Lasa M., Polevoda B., Gazquez C., Elosegui-Artola A., Kim D.S., De Juan-Pardo E., Demeyer K., Hole K., Larrea E., Timmerman E., Prieto J., Arnesen T., Sherman F., Gevaert K., Aldabe R.
Proc. Natl. Acad. Sci. U.S.A. 109:12449-12454(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT SER-2, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS], CLEAVAGE OF INITIATOR METHIONINE [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U00030 Genomic DNA. Translation: AAB68356.1.
BK006934 Genomic DNA. Translation: DAA06889.2.
PIRS46678.
RefSeqNP_012067.4. NM_001179328.4.

3D structure databases

ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid36631. 97 interactions.
DIPDIP-804N.
IntActP38883. 45 interactions.
MINTMINT-403308.
STRING4932.YHR197W.

Proteomic databases

PaxDbP38883.
PeptideAtlasP38883.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYHR197W; YHR197W; YHR197W.
GeneID856604.
KEGGsce:YHR197W.

Organism-specific databases

CYGDYHR197w.
SGDS000001240. RIX1.

Phylogenomic databases

eggNOGNOG45551.
HOGENOMHOG000154134.
KOK14828.
OMAWCGINLI.
OrthoDBEOG7MKWG4.

Enzyme and pathway databases

BioCycYEAST:G3O-31225-MONOMER.

Gene expression databases

GenevestigatorP38883.

Family and domain databases

ProtoNetSearch...

Other

NextBio982508.

Entry information

Entry nameRIX1_YEAST
AccessionPrimary (citable) accession number: P38883
Secondary accession number(s): D3DLE5
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: July 27, 2011
Last modified: April 16, 2014
This is version 100 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome VIII

Yeast (Saccharomyces cerevisiae) chromosome VIII: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families