P38882 (UTP9_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
U3 small nucleolar RNA-associated protein 9
Short name=U3 snoRNA-associated protein 9

Alternative name(s):
U three protein 9
U3 protein 9 required for transcription
t-UTP9

Gene names

Name:	UTP9
Ordered Locus Names:	YHR196W

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › Saccharomyces cerevisiae

Protein attributes

Sequence length	575 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Involved in nucleolar processing of pre-18S ribosomal RNA. Required for optimal pre-ribosomal RNA transcription by RNA polymerase I together with a subset of U3 proteins required for transcription (t-UTPs). Ref.3 Ref.5
Subunit structure	Interacts with snoRNA U3. Interacts with MPP10. Component of the ribosomal small subunit (SSU) processome composed of at least 40 protein subunits and snoRNA U3. In the absence of snoRNA3, forms a complex with other t-UTPs. This complex can associate with pre-18S ribosomal RNAs. Ref.3 Ref.5
Subcellular location	Nucleus › nucleolus. Note: Associated with ribosomal chromatin, even in the absence of transcription. Ref.3 Ref.5
Miscellaneous	Present with 20000 molecules/cell in log phase SD medium.

Ontologies

Keywords
Biological process	Ribosome biogenesis Transcription rRNA processing
Cellular component	Nucleus
Molecular function	Ribonucleoprotein
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) Inferred from mutant phenotype Ref.3. Source: SGD positive regulation of transcription from RNA polymerase I promoter Inferred from mutant phenotype Ref.5. Source: SGD transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	rDNA heterochromatin Inferred from direct assay Ref.5. Source: SGD small-subunit processome Inferred from direct assay Ref.3. Source: SGD t-UTP complex Inferred from direct assay PubMed 17515605. Source: SGD
Molecular_function	snoRNA binding Inferred from physical interaction Ref.3. Source: SGD
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
UTP15	Q04305	4	EBI-24892,EBI-28183
UTP8	P53276	3	EBI-24892,EBI-23301

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 575	575	U3 small nucleolar RNA-associated protein 9		PRO_0000065744
Amino acid modifications
	Modified residue	547	1	Phosphoserine Ref.6
	Modified residue	564	1	Phosphoserine Ref.6

Sequences

	Sequence		Length	Mass (Da)
	P38882 [UniParc]. Last modified February 1, 1995. Version 1. Checksum: 0C482C6A32855FF1	FASTA	575	65,271
	10 20 30 40 50 60 MGSSLDLVAS FSHDSTRFAF QASVAQKNNV DIYPLNETKD YVVNSSLVSH IDYETNDMKV 70 80 90 100 110 120 SDVIFFGWCS DLIDTQSSNI KRKLDEDEGT GESSEQRCEN FFVNGFPDGR IVVYSSNGKD 130 140 150 160 170 180 IVNIIKNKKE ILGADTDESD IWILDSDKVV KKLQYNNSKP LKTFTLVDGK DDEIVHFQIL 190 200 210 220 230 240 HQNGTLLVCI ITKQMVYIVD PSKRRPSTKY SFEISDAVAC EFSSDGKYLL IANNEELIAY 250 260 270 280 290 300 DLKEDSKLIQ SWPVQVKTLK TLDDLIMALT TDGKINNYKI GEADKVCSIV VNEDLEIIDF 310 320 330 340 350 360 TPINSKQQVL ISWLNVNEPN FESISLKEIE TQGYITINKN EKNNADEADQ KKLEEKEEEA 370 380 390 400 410 420 QPEVQHEKKE TETKINKKVS KSDQVEIANI LSSHLEANST EILDDLMSGS WTEPEIKKFI 430 440 450 460 470 480 LTKINTVDHL SKIFLTISKS ITQNPWNEEN LLPLWLKWLL TLKSGELNSI KDKHTKKNCK 490 500 510 520 530 540 HLKSALRSSE EILPVLLGIQ GRLEMLRRQA KLREDLAQLS MQEGEDDEIE VIEHSNVISN 550 560 570 PLQDQASPVE KLEPDSIVYA NGESDEFVDA SEYKD

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Complete nucleotide sequence of Saccharomyces cerevisiae chromosome VIII." Johnston M., Andrews S., Brinkman R., Cooper J., Ding H., Dover J., Du Z., Favello A., Fulton L., Gattung S., Geisel C., Kirsten J., Kucaba T., Hillier L.W., Jier M., Johnston L., Langston Y., Latreille P. , Louis E.J., Macri C., Mardis E., Menezes S., Mouser L., Nhan M., Rifkin L., Riles L., St Peter H., Trevaskis E., Vaughan K., Vignati D., Wilcox L., Wohldman P., Waterston R., Wilson R., Vaudin M. Science 265:2077-2082(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[2]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[3]	"A large nucleolar U3 ribonucleoprotein required for 18S ribosomal RNA biogenesis." Dragon F., Gallagher J.E.G., Compagnone-Post P.A., Mitchell B.M., Porwancher K.A., Wehner K.A., Wormsley S., Settlage R.E., Shabanowitz J., Osheim Y., Beyer A.L., Hunt D.F., Baserga S.J. Nature 417:967-970(2002) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, INTERACTION WITH MPP10 AND SNORNA U3, IDENTIFICATION IN SSU PROCESSOME BY MASS SPECTROMETRY, SUBCELLULAR LOCATION.
[4]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[5]	"RNA polymerase I transcription and pre-rRNA processing are linked by specific SSU processome components." Gallagher J.E.G., Dunbar D.A., Granneman S., Mitchell B.M., Osheim Y., Beyer A.L., Baserga S.J. Genes Dev. 18:2506-2517(2004) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, IDENTIFICATION IN COMPLEX WITH OTHER T-UTPS, SUBCELLULAR LOCATION.
[6]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-547 AND SER-564, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U00030 Genomic DNA. Translation: AAB68369.1. BK006934 Genomic DNA. Translation: DAA06888.1.
PIR	S46692.
RefSeq	NP_012066.1. NM_001179327.1.
3D structure databases
ProteinModelPortal	P38882.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-6634N.
IntAct	P38882. 57 interactions.
MINT	MINT-628324.
STRING	4932.YHR196W.
Proteomic databases
PaxDb	P38882.
PeptideAtlas	P38882.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YHR196W; YHR196W; YHR196W.
GeneID	856603.
KEGG	sce:YHR196W.
Organism-specific databases
CYGD	YHR196w.
SGD	S000001239. UTP9.
Phylogenomic databases
eggNOG	NOG47655.
HOGENOM	HOG000000891.
KO	K14551.
OMA	NVNEPNF.
OrthoDB	EOG4V1B99.
Gene expression databases
Genevestigator	P38882.
GermOnline	YHR196W. Saccharomyces cerevisiae.
Family and domain databases
Gene3D	2.130.10.10. 1 hit.
InterPro	IPR015943. WD40/YVTN_repeat-like_dom. [Graphical view]
ProtoNet	Search...
Other
NextBio	982505.

Entry information

Entry name

UTP9_YEAST

Accession

Primary (citable) accession number: P38882
Secondary accession number(s): D3DLE4

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1995
Last sequence update:	February 1, 1995
Last modified:	April 3, 2013
This is version 90 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program