P38881 (NVJ1_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Nucleus-vacuole junction protein 1

Gene names

Name:	NVJ1
Synonyms:	VAB36
Ordered Locus Names:	YHR195W

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › Saccharomyces cerevisiae

Protein attributes

Sequence length	321 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Involved in the formation of nucleus-vacuole (NV) junctions during piecemeal microautophagy of the nucleus (PMN). NV junctions are interorganelle interfaces mediated by NVJ1 in the nuclear envelope and VAC8 on the vacuole membrane. Together, NVJ1 and VAC8 form Velcro-like patches through which teardrop-like portions of the nucleus are pinched off into the vacuolar lumen and degraded by the PMN process. Acts also as an outer-nuclear membrane receptor for OSH1 and TSC13. Ref.3 Ref.4 Ref.7 Ref.8 Ref.9
Subunit structure	Interacts with OSH1, TSC13 and VAC8. Ref.3 Ref.7 Ref.8 Ref.9
Subcellular location	Nucleus outer membrane; Single-pass membrane protein Ref.3 Ref.5 Ref.7 Ref.9.
Miscellaneous	Present with 1900 molecules/cell in log phase SD medium.

Ontologies

Keywords
Biological process	Autophagy
Cellular component	Membrane Nucleus
Domain	Signal Transmembrane Transmembrane helix
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	cellular protein localization Inferred from mutant phenotype Ref.9. Source: SGD microautophagy Inferred from mutant phenotype Ref.4. Source: SGD
Cellular_component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW nuclear envelope Inferred from direct assay Ref.3. Source: SGD nuclear outer membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
VAC8	P39968	4	EBI-24885,EBI-20212

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 22	22	Potential
	Chain	23 – 321	299	Nucleus-vacuole junction protein 1		PRO_0000202937
Regions
	Transmembrane	94 – 114	21	Helical; Potential
	Region	73 – 125	53	TSC13-binding
	Region	139 – 195	57	OSH1-binding
	Region	233 – 321	89	VAC8-binding
	Compositional bias	235 – 303	69	Ser-rich
Amino acid modifications
	Modified residue	156	1	Phosphoserine Ref.10 Ref.11 Ref.12
	Modified residue	190	1	Phosphoserine Ref.12
	Modified residue	199	1	Phosphoserine Ref.12
	Modified residue	250	1	Phosphoserine Ref.11
	Modified residue	251	1	Phosphothreonine Ref.11 Ref.12
	Modified residue	272	1	Phosphoserine Ref.12
	Modified residue	285	1	Phosphoserine Ref.12
	Modified residue	298	1	Phosphoserine Ref.12

Sequences

	Sequence		Length	Mass (Da)
	P38881 [UniParc]. Last modified February 1, 1995. Version 1. Checksum: 13F54DFE8D1E5875	FASTA	321	36,421
	10 20 30 40 50 60 MTRPPLVRGI FSLGLSVAVL KGVEKTVRKH LERQGWIEPQ KVDYELIFTI DRLKNLVDNK 70 80 90 100 110 120 REALTAEQPD AGELSWRKVF NFISRQSSEL DTRIYVLILL LSFLLPIAWT VLDGDRETTL 130 140 150 160 170 180 EDKDNDCNVD LIENERRLKH YNDGERAVLQ FGKNRSEPII LSYKDMNVLE GEHEFTSKEE 190 200 210 220 230 240 HSNSHLTSKS ENALNQVGSE DLLGCHLEKQ LEEDKNEPNG EADGEDDNNR EKDCSSSSEV 250 260 270 280 290 300 ESQSKCRKES TAEPDSLSRD TRTTSSLKSS TSFPISFKGS IDLKSLNQPS SLLHIQVSPT 310 320 KSSNLDAQVN TEQAYSQPFR Y

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Complete nucleotide sequence of Saccharomyces cerevisiae chromosome VIII." Johnston M., Andrews S., Brinkman R., Cooper J., Ding H., Dover J., Du Z., Favello A., Fulton L., Gattung S., Geisel C., Kirsten J., Kucaba T., Hillier L.W., Jier M., Johnston L., Langston Y., Latreille P. , Louis E.J., Macri C., Mardis E., Menezes S., Mouser L., Nhan M., Rifkin L., Riles L., St Peter H., Trevaskis E., Vaughan K., Vignati D., Wilcox L., Wohldman P., Waterston R., Wilson R., Vaudin M. Science 265:2077-2082(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[2]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[3]	"Nucleus-vacuole junctions in Saccharomyces cerevisiae are formed through the direct interaction of Vac8p with Nvj1p." Pan X., Roberts P., Chen Y., Kvam E., Shulga N., Huang K., Lemmon S., Goldfarb D.S. Mol. Biol. Cell 11:2445-2457(2000) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH VAC8, SUBCELLULAR LOCATION, FUNCTION.
[4]	"Piecemeal microautophagy of nucleus in Saccharomyces cerevisiae." Roberts P., Moshitch-Moshkovitz S., Kvam E., O'Toole E., Winey M., Goldfarb D.S. Mol. Biol. Cell 14:129-141(2003) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
[5]	"Global analysis of protein localization in budding yeast." Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K. Nature 425:686-691(2003) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[6]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[7]	"Nvj1p is the outer-nuclear-membrane receptor for oxysterol-binding protein homolog Osh1p in Saccharomyces cerevisiae." Kvam E., Goldfarb D.S. J. Cell Sci. 117:4959-4968(2004) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH OSH1, SUBCELLULAR LOCATION, FUNCTION.
[8]	"Targeting of Tsc13p to nucleus-vacuole junctions: a role for very-long-chain fatty acids in the biogenesis of microautophagic vesicles." Kvam E., Gable K., Dunn T.M., Goldfarb D.S. Mol. Biol. Cell 16:3987-3998(2005) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH TSC13, FUNCTION.
[9]	"Structure and function of nucleus-vacuole junctions: outer-nuclear-membrane targeting of Nvj1p and a role in tryptophan uptake." Kvam E., Goldfarb D.S. J. Cell Sci. 119:3622-3633(2006) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, DOMAINS, SUBCELLULAR LOCATION, INTERACTION WITH OSH1; TSC13 AND VAC8.
[10]	"Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae." Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P. J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-156, MASS SPECTROMETRY. Strain: ADR376.
[11]	"Proteome-wide identification of in vivo targets of DNA damage checkpoint kinases." Smolka M.B., Albuquerque C.P., Chen S.H., Zhou H. Proc. Natl. Acad. Sci. U.S.A. 104:10364-10369(2007) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-156; SER-250 AND THR-251, MASS SPECTROMETRY.
[12]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-156; SER-190; SER-199; THR-251; SER-272; SER-285 AND SER-298, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U00030 Genomic DNA. Translation: AAB68357.1. BK006934 Genomic DNA. Translation: DAA06887.1.
PIR	S46679.
RefSeq	NP_012065.3. NM_001179326.3.
3D structure databases
ProteinModelPortal	P38881.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-4215N.
IntAct	P38881. 3 interactions.
MINT	MINT-508720.
STRING	4932.YHR195W.
Proteomic databases
PaxDb	P38881.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YHR195W; YHR195W; YHR195W.
GeneID	856602.
KEGG	sce:YHR195W. sce:YHR197W.
Organism-specific databases
CYGD	YHR195w.
SGD	S000001238. NVJ1.
Phylogenomic databases
eggNOG	NOG266864.
KO	K14828.
OrthoDB	EOG41NXXG.
Gene expression databases
Genevestigator	P38881.
GermOnline	YHR195W. Saccharomyces cerevisiae.
Family and domain databases
ProtoNet	Search...
Other
NextBio	982502.

Entry information

Entry name

NVJ1_YEAST

Accession

Primary (citable) accession number: P38881
Secondary accession number(s): D3DLE3

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1995
Last sequence update:	February 1, 1995
Last modified:	April 3, 2013
This is version 87 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program