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P38881 (NVJ1_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified March 19, 2014. Version 93. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Nucleus-vacuole junction protein 1
Gene names
Name:NVJ1
Synonyms:VAB36
Ordered Locus Names:YHR195W
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length321 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Involved in the formation of nucleus-vacuole (NV) junctions during piecemeal microautophagy of the nucleus (PMN). NV junctions are interorganelle interfaces mediated by NVJ1 in the nuclear envelope and VAC8 on the vacuole membrane. Together, NVJ1 and VAC8 form Velcro-like patches through which teardrop-like portions of the nucleus are pinched off into the vacuolar lumen and degraded by the PMN process. Acts also as an outer-nuclear membrane receptor for OSH1 and TSC13. Ref.3 Ref.4 Ref.7 Ref.8 Ref.9

Subunit structure

Interacts with OSH1, TSC13 and VAC8. Ref.3 Ref.7 Ref.8 Ref.9

Subcellular location

Nucleus outer membrane; Single-pass membrane protein Ref.3 Ref.5 Ref.7 Ref.9.

Miscellaneous

Present with 1900 molecules/cell in log phase SD medium.

Binary interactions

With

Entry

#Exp.

IntAct

Notes

VAC8P399684EBI-24885,EBI-20212

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2222 Potential
Chain23 – 321299Nucleus-vacuole junction protein 1
PRO_0000202937

Regions

Transmembrane94 – 11421Helical; Potential
Region73 – 12553TSC13-binding
Region139 – 19557OSH1-binding
Region233 – 32189VAC8-binding
Compositional bias235 – 30369Ser-rich

Amino acid modifications

Modified residue1561Phosphoserine Ref.10 Ref.11
Modified residue1991Phosphoserine Ref.11
Modified residue2851Phosphoserine Ref.11 Ref.12
Modified residue2981Phosphoserine Ref.12

Sequences

Sequence LengthMass (Da)Tools
P38881 [UniParc].

Last modified February 1, 1995. Version 1.
Checksum: 13F54DFE8D1E5875

FASTA32136,421
        10         20         30         40         50         60 
MTRPPLVRGI FSLGLSVAVL KGVEKTVRKH LERQGWIEPQ KVDYELIFTI DRLKNLVDNK 

        70         80         90        100        110        120 
REALTAEQPD AGELSWRKVF NFISRQSSEL DTRIYVLILL LSFLLPIAWT VLDGDRETTL 

       130        140        150        160        170        180 
EDKDNDCNVD LIENERRLKH YNDGERAVLQ FGKNRSEPII LSYKDMNVLE GEHEFTSKEE 

       190        200        210        220        230        240 
HSNSHLTSKS ENALNQVGSE DLLGCHLEKQ LEEDKNEPNG EADGEDDNNR EKDCSSSSEV 

       250        260        270        280        290        300 
ESQSKCRKES TAEPDSLSRD TRTTSSLKSS TSFPISFKGS IDLKSLNQPS SLLHIQVSPT 

       310        320 
KSSNLDAQVN TEQAYSQPFR Y 

« Hide

References

« Hide 'large scale' references
[1]"Complete nucleotide sequence of Saccharomyces cerevisiae chromosome VIII."
Johnston M., Andrews S., Brinkman R., Cooper J., Ding H., Dover J., Du Z., Favello A., Fulton L., Gattung S., Geisel C., Kirsten J., Kucaba T., Hillier L.W., Jier M., Johnston L., Langston Y., Latreille P. expand/collapse author list , Louis E.J., Macri C., Mardis E., Menezes S., Mouser L., Nhan M., Rifkin L., Riles L., St Peter H., Trevaskis E., Vaughan K., Vignati D., Wilcox L., Wohldman P., Waterston R., Wilson R., Vaudin M.
Science 265:2077-2082(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[2]Saccharomyces Genome Database
Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[3]"Nucleus-vacuole junctions in Saccharomyces cerevisiae are formed through the direct interaction of Vac8p with Nvj1p."
Pan X., Roberts P., Chen Y., Kvam E., Shulga N., Huang K., Lemmon S., Goldfarb D.S.
Mol. Biol. Cell 11:2445-2457(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH VAC8, SUBCELLULAR LOCATION, FUNCTION.
[4]"Piecemeal microautophagy of nucleus in Saccharomyces cerevisiae."
Roberts P., Moshitch-Moshkovitz S., Kvam E., O'Toole E., Winey M., Goldfarb D.S.
Mol. Biol. Cell 14:129-141(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[5]"Global analysis of protein localization in budding yeast."
Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K.
Nature 425:686-691(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[6]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[7]"Nvj1p is the outer-nuclear-membrane receptor for oxysterol-binding protein homolog Osh1p in Saccharomyces cerevisiae."
Kvam E., Goldfarb D.S.
J. Cell Sci. 117:4959-4968(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH OSH1, SUBCELLULAR LOCATION, FUNCTION.
[8]"Targeting of Tsc13p to nucleus-vacuole junctions: a role for very-long-chain fatty acids in the biogenesis of microautophagic vesicles."
Kvam E., Gable K., Dunn T.M., Goldfarb D.S.
Mol. Biol. Cell 16:3987-3998(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH TSC13, FUNCTION.
[9]"Structure and function of nucleus-vacuole junctions: outer-nuclear-membrane targeting of Nvj1p and a role in tryptophan uptake."
Kvam E., Goldfarb D.S.
J. Cell Sci. 119:3622-3633(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, DOMAINS, SUBCELLULAR LOCATION, INTERACTION WITH OSH1; TSC13 AND VAC8.
[10]"Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-156, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Strain: ADR376.
[11]"A multidimensional chromatography technology for in-depth phosphoproteome analysis."
Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-156; SER-199 AND SER-285, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[12]"Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-285 AND SER-298, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U00030 Genomic DNA. Translation: AAB68357.1.
BK006934 Genomic DNA. Translation: DAA06887.1.
PIRS46679.
RefSeqNP_012065.3. NM_001179326.3.

3D structure databases

ProteinModelPortalP38881.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid36629. 28 interactions.
DIPDIP-4215N.
IntActP38881. 3 interactions.
MINTMINT-508720.
STRING4932.YHR195W.

Proteomic databases

PaxDbP38881.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYHR195W; YHR195W; YHR195W.
GeneID856602.
KEGGsce:YHR195W.

Organism-specific databases

CYGDYHR195w.
SGDS000001238. NVJ1.

Phylogenomic databases

eggNOGNOG266864.
OrthoDBEOG7T1RNH.

Enzyme and pathway databases

BioCycYEAST:G3O-31223-MONOMER.

Gene expression databases

GenevestigatorP38881.

Family and domain databases

ProtoNetSearch...

Other

NextBio982502.

Entry information

Entry nameNVJ1_YEAST
AccessionPrimary (citable) accession number: P38881
Secondary accession number(s): D3DLE3
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: February 1, 1995
Last modified: March 19, 2014
This is version 93 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome VIII

Yeast (Saccharomyces cerevisiae) chromosome VIII: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD