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Protein

Nascent polypeptide-associated complex subunit alpha

Gene

EGD2

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Component of the nascent polypeptide-associated complex (NAC), a dynamic component of the ribosomal exit tunnel, protecting the emerging polypeptides from interaction with other cytoplasmic proteins to ensure appropriate nascent protein targeting. The NAC complex also promotes mitochondrial protein import by enhancing productive ribosome interactions with the outer mitochondrial membrane and blocks the inappropriate interaction of ribosomes translating non-secretory nascent polypeptides with translocation sites in the membrane of the endoplasmic reticulum. EGD2 may also be involved in transcription regulation.6 Publications

GO - Molecular functioni

  • phosphatidic acid binding Source: SGD
  • phosphatidylinositol-3,5-bisphosphate binding Source: SGD
  • phosphatidylinositol-3-phosphate binding Source: SGD
  • phosphatidylinositol-4-phosphate binding Source: SGD
  • unfolded protein binding Source: SGD

GO - Biological processi

  • 'de novo' cotranslational protein folding Source: SGD
  • posttranslational protein targeting to membrane Source: SGD
Complete GO annotation...

Keywords - Biological processi

Protein transport, Transport

Enzyme and pathway databases

BioCyciYEAST:G3O-31221-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Nascent polypeptide-associated complex subunit alpha
Short name:
NAC-alpha
Alternative name(s):
Alpha-NAC
GAL4 DNA-binding enhancer protein 2
Gene namesi
Name:EGD2
Ordered Locus Names:YHR193C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome VIII

Organism-specific databases

EuPathDBiFungiDB:YHR193C.
SGDiS000001236. EGD2.

Subcellular locationi

  • Cytoplasm
  • Nucleus

  • Note: Predominantly cytoplasmic, may also transiently localize to the nucleus. Nuclear import may occur via redundant pathways including one requiring the karyopherins PSE1 and KAP123, which also participate in nuclear import of ribosomal subunits.

GO - Cellular componenti

  • nascent polypeptide-associated complex Source: SGD
  • nucleus Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedCombined sources1 Publication
ChainiPRO_00001355942 – 174Nascent polypeptide-associated complex subunit alphaAdd BLAST173

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylserineCombined sources1 Publication1
Modified residuei93PhosphoserineCombined sources1

Post-translational modificationi

Ubiquitinated by the NOT4 E3 ligase and the UBC4 E2 ubiquitin conjugatin enzyme.1 Publication

Keywords - PTMi

Acetylation, Phosphoprotein, Ubl conjugation

Proteomic databases

MaxQBiP38879.
PRIDEiP38879.
TopDownProteomicsiP38879.

PTM databases

iPTMnetiP38879.

Interactioni

Subunit structurei

Part of the nascent polypeptide-associated complex (NAC), consisting of EGD2 and either EGD1 or BTT1. NAC associates with ribosomes via EGD1 or BTT1, and with the CCR4-NOT complex.

Binary interactionsi

WithEntry#Exp.IntActNotes
EGD1Q026427EBI-6379,EBI-6371
RPL4AP106642EBI-6379,EBI-15390
UBC4P157312EBI-6379,EBI-19735

GO - Molecular functioni

  • unfolded protein binding Source: SGD

Protein-protein interaction databases

BioGridi36627. 79 interactors.
DIPiDIP-2098N.
IntActiP38879. 51 interactors.
MINTiMINT-537224.

Structurei

3D structure databases

ProteinModelPortaliP38879.
SMRiP38879.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini15 – 73NAC-A/BPROSITE-ProRule annotationAdd BLAST59
Domaini135 – 174UBAAdd BLAST40

Domaini

The UBA domain is required for the stability of EGD1.

Sequence similaritiesi

Belongs to the NAC-alpha family.Curated
Contains 1 NAC-A/B (NAC-alpha/beta) domain.PROSITE-ProRule annotation
Contains 1 UBA domain.Curated

Phylogenomic databases

GeneTreeiENSGT00440000033468.
HOGENOMiHOG000239674.
InParanoidiP38879.
KOiK03626.
OMAiIMPSNED.
OrthoDBiEOG092C57O4.

Family and domain databases

InterProiIPR016641. EGD2/NACA.
IPR002715. Nas_poly-pep-assoc_cplx_dom.
IPR009060. UBA-like.
[Graphical view]
PANTHERiPTHR21713. PTHR21713. 1 hit.
PfamiPF01849. NAC. 1 hit.
[Graphical view]
PIRSFiPIRSF015901. NAC_alpha. 1 hit.
SMARTiSM01407. NAC. 1 hit.
[Graphical view]
SUPFAMiSSF46934. SSF46934. 1 hit.
PROSITEiPS51151. NAC_AB. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P38879-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSAIPENANV TVLNKNEKKA RELIGKLGLK QIPGIIRVTF RKKDNQIYAI
60 70 80 90 100
EKPEVFRSAG GNYVVFGEAK VDNFTQKLAA AQQQAQASGI MPSNEDVATK
110 120 130 140 150
SPEDIQADMQ AAAEGSVNAA AEEDDEEGEV DAGDLNKDDI ELVVQQTNVS
160 170
KNQAIKALKA HNGDLVNAIM SLSK
Length:174
Mass (Da):18,709
Last modified:January 23, 2007 - v3
Checksum:i86CE0C4E6194C286
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U17134 Genomic DNA. Translation: AAA92080.1.
U00030 Genomic DNA. Translation: AAB68367.1.
AY558288 Genomic DNA. Translation: AAS56614.1.
L12222 mRNA. Translation: AAC15849.1.
BK006934 Genomic DNA. Translation: DAA06885.1.
PIRiS46689.
RefSeqiNP_012063.3. NM_001179324.3.

Genome annotation databases

EnsemblFungiiYHR193C; YHR193C; YHR193C.
GeneIDi856600.
KEGGisce:YHR193C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U17134 Genomic DNA. Translation: AAA92080.1.
U00030 Genomic DNA. Translation: AAB68367.1.
AY558288 Genomic DNA. Translation: AAS56614.1.
L12222 mRNA. Translation: AAC15849.1.
BK006934 Genomic DNA. Translation: DAA06885.1.
PIRiS46689.
RefSeqiNP_012063.3. NM_001179324.3.

3D structure databases

ProteinModelPortaliP38879.
SMRiP38879.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi36627. 79 interactors.
DIPiDIP-2098N.
IntActiP38879. 51 interactors.
MINTiMINT-537224.

PTM databases

iPTMnetiP38879.

Proteomic databases

MaxQBiP38879.
PRIDEiP38879.
TopDownProteomicsiP38879.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYHR193C; YHR193C; YHR193C.
GeneIDi856600.
KEGGisce:YHR193C.

Organism-specific databases

EuPathDBiFungiDB:YHR193C.
SGDiS000001236. EGD2.

Phylogenomic databases

GeneTreeiENSGT00440000033468.
HOGENOMiHOG000239674.
InParanoidiP38879.
KOiK03626.
OMAiIMPSNED.
OrthoDBiEOG092C57O4.

Enzyme and pathway databases

BioCyciYEAST:G3O-31221-MONOMER.

Miscellaneous databases

PROiP38879.

Family and domain databases

InterProiIPR016641. EGD2/NACA.
IPR002715. Nas_poly-pep-assoc_cplx_dom.
IPR009060. UBA-like.
[Graphical view]
PANTHERiPTHR21713. PTHR21713. 1 hit.
PfamiPF01849. NAC. 1 hit.
[Graphical view]
PIRSFiPIRSF015901. NAC_alpha. 1 hit.
SMARTiSM01407. NAC. 1 hit.
[Graphical view]
SUPFAMiSSF46934. SSF46934. 1 hit.
PROSITEiPS51151. NAC_AB. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiNACA_YEAST
AccessioniPrimary (citable) accession number: P38879
Secondary accession number(s): D3DLE1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: January 23, 2007
Last modified: November 30, 2016
This is version 154 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 38016 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome VIII
    Yeast (Saccharomyces cerevisiae) chromosome VIII: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.