ID CTF8_YEAST Reviewed; 133 AA. AC P38877; D3DLD9; DT 01-FEB-1995, integrated into UniProtKB/Swiss-Prot. DT 01-FEB-1995, sequence version 1. DT 27-MAR-2024, entry version 165. DE RecName: Full=Chromosome transmission fidelity protein 8; GN Name=CTF8; OrderedLocusNames=YHR191C; OS Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast). OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; OC Saccharomycetales; Saccharomycetaceae; Saccharomyces. OX NCBI_TaxID=559292; RN [1] RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, IDENTIFICATION IN THE RP CTF18-RFC COMPLEX, AND SUBCELLULAR LOCATION. RX PubMed=11389843; DOI=10.1016/s1097-2765(01)00254-4; RA Mayer M.L., Gygi S.P., Aebersold R., Hieter P.; RT "Identification of RFC(Ctf18p, Ctf8p, Dcc1p): an alternative RFC complex RT required for sister chromatid cohesion in S. cerevisiae."; RL Mol. Cell 7:959-970(2001). RN [2] RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RC STRAIN=ATCC 204508 / S288c; RX PubMed=8091229; DOI=10.1126/science.8091229; RA Johnston M., Andrews S., Brinkman R., Cooper J., Ding H., Dover J., Du Z., RA Favello A., Fulton L., Gattung S., Geisel C., Kirsten J., Kucaba T., RA Hillier L.W., Jier M., Johnston L., Langston Y., Latreille P., Louis E.J., RA Macri C., Mardis E., Menezes S., Mouser L., Nhan M., Rifkin L., Riles L., RA St Peter H., Trevaskis E., Vaughan K., Vignati D., Wilcox L., Wohldman P., RA Waterston R., Wilson R., Vaudin M.; RT "Complete nucleotide sequence of Saccharomyces cerevisiae chromosome RT VIII."; RL Science 265:2077-2082(1994). RN [3] RP GENOME REANNOTATION. RC STRAIN=ATCC 204508 / S288c; RX PubMed=24374639; DOI=10.1534/g3.113.008995; RA Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R., RA Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S., RA Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.; RT "The reference genome sequence of Saccharomyces cerevisiae: Then and now."; RL G3 (Bethesda) 4:389-398(2014). RN [4] RP NUCLEOTIDE SEQUENCE [GENOMIC DNA]. RC STRAIN=ATCC 204508 / S288c; RX PubMed=17322287; DOI=10.1101/gr.6037607; RA Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., RA Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., RA Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J., RA Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., RA LaBaer J.; RT "Approaching a complete repository of sequence-verified protein-encoding RT clones for Saccharomyces cerevisiae."; RL Genome Res. 17:536-543(2007). RN [5] RP IDENTIFICATION IN THE CTF18-RFC COMPLEX, AND FUNCTION OF THE CTF18-RFC RP COMPLEX. RX PubMed=15964801; DOI=10.1128/mcb.25.13.5445-5455.2005; RA Bylund G.O., Burgers P.M.; RT "Replication protein A-directed unloading of PCNA by the Ctf18 cohesion RT establishment complex."; RL Mol. Cell. Biol. 25:5445-5455(2005). CC -!- FUNCTION: Essential for the fidelity of chromosome transmission. CC Required for the DNA replication block checkpoint. Component of the CC RFC-like complex CTF18-RFC which is required for efficient CC establishment of chromosome cohesion during S-phase and may load or CC unload POL30/PCNA. During a clamp loading circle, the RFC:clamp complex CC binds to DNA and the recognition of the double-stranded/single-stranded CC junction stimulates ATP hydrolysis by RFC. The complex presumably CC provides bipartite ATP sites in which one subunit supplies a catalytic CC site for hydrolysis of ATP bound to the neighboring subunit. CC Dissociation of RFC from the clamp leaves the clamp encircling DNA. CC {ECO:0000269|PubMed:11389843, ECO:0000269|PubMed:15964801}. CC -!- SUBUNIT: Component of the CTF18-RFC complex, which consists of CTF18, CC CTF8, DSCC1, RFC2, RFC3, RFC4 and RFC5. {ECO:0000269|PubMed:11389843, CC ECO:0000269|PubMed:15964801}. CC -!- INTERACTION: CC P38877; P49956: CTF18; NbExp=4; IntAct=EBI-5216, EBI-4560; CC P38877; P25559: DCC1; NbExp=2; IntAct=EBI-5216, EBI-5661; CC P38877; P40348: RFC2; NbExp=2; IntAct=EBI-5216, EBI-14992; CC P38877; P38629: RFC3; NbExp=2; IntAct=EBI-5216, EBI-15000; CC P38877; P40339: RFC4; NbExp=2; IntAct=EBI-5216, EBI-15009; CC P38877; P38251: RFC5; NbExp=2; IntAct=EBI-5216, EBI-15016; CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:11389843}. CC Note=Associates with chromatin. CC -!- SIMILARITY: Belongs to the CTF8 family. {ECO:0000305}. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; U53671; AAA98983.1; -; Genomic_DNA. DR EMBL; U00030; AAB68366.1; -; Genomic_DNA. DR EMBL; AY558287; AAS56613.1; -; Genomic_DNA. DR EMBL; BK006934; DAA06883.1; -; Genomic_DNA. DR PIR; S46688; S46688. DR RefSeq; NP_012061.3; NM_001179322.3. DR PDB; 5MSM; X-ray; 2.29 A; B/E=1-133. DR PDB; 5OKC; X-ray; 2.30 A; F/H=1-133. DR PDB; 5OKI; X-ray; 4.50 A; D/H=1-133. DR PDB; 6S1C; X-ray; 6.10 A; C/G=1-133. DR PDB; 6S2E; EM; 4.20 A; D=1-133. DR PDB; 6S2F; EM; 5.80 A; D=1-133. DR PDBsum; 5MSM; -. DR PDBsum; 5OKC; -. DR PDBsum; 5OKI; -. DR PDBsum; 6S1C; -. DR PDBsum; 6S2E; -. DR PDBsum; 6S2F; -. DR AlphaFoldDB; P38877; -. DR EMDB; EMD-10088; -. DR EMDB; EMD-10089; -. DR SMR; P38877; -. DR BioGRID; 36625; 654. DR ComplexPortal; CPX-1731; CTF18-RFC complex. DR DIP; DIP-2727N; -. DR IntAct; P38877; 8. DR MINT; P38877; -. DR STRING; 4932.YHR191C; -. DR MaxQB; P38877; -. DR PaxDb; 4932-YHR191C; -. DR PeptideAtlas; P38877; -. DR EnsemblFungi; YHR191C_mRNA; YHR191C; YHR191C. DR GeneID; 856598; -. DR KEGG; sce:YHR191C; -. DR AGR; SGD:S000001234; -. DR SGD; S000001234; CTF8. DR VEuPathDB; FungiDB:YHR191C; -. DR eggNOG; KOG4487; Eukaryota. DR HOGENOM; CLU_090690_1_0_1; -. DR InParanoid; P38877; -. DR OMA; QRPLPIM; -. DR OrthoDB; 1972318at2759; -. DR BioCyc; YEAST:G3O-31219-MONOMER; -. DR BioGRID-ORCS; 856598; 9 hits in 10 CRISPR screens. DR PRO; PR:P38877; -. DR Proteomes; UP000002311; Chromosome VIII. DR RNAct; P38877; Protein. DR GO; GO:0031390; C:Ctf18 RFC-like complex; IPI:SGD. DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW. DR GO; GO:0006260; P:DNA replication; IEA:UniProtKB-KW. DR GO; GO:0035753; P:maintenance of DNA trinucleotide repeats; IMP:SGD. DR GO; GO:0007064; P:mitotic sister chromatid cohesion; IMP:SGD. DR InterPro; IPR018607; Ctf8. DR PANTHER; PTHR28605; CTF8, CHROMOSOME TRANSMISSION FIDELITY FACTOR 8 HOMOLOG (S. CEREVISIAE); 1. DR PANTHER; PTHR28605:SF1; CTF8, CHROMOSOME TRANSMISSION FIDELITY FACTOR 8 HOMOLOG (S. CEREVISIAE); 1. DR Pfam; PF09696; Ctf8; 1. PE 1: Evidence at protein level; KW 3D-structure; Cell cycle; DNA replication; DNA-binding; Nucleus; KW Reference proteome. FT CHAIN 1..133 FT /note="Chromosome transmission fidelity protein 8" FT /id="PRO_0000079494" FT STRAND 3..7 FT /evidence="ECO:0007829|PDB:5MSM" FT HELIX 9..14 FT /evidence="ECO:0007829|PDB:5MSM" FT STRAND 22..25 FT /evidence="ECO:0007829|PDB:5MSM" FT STRAND 29..40 FT /evidence="ECO:0007829|PDB:5MSM" FT HELIX 45..50 FT /evidence="ECO:0007829|PDB:5MSM" FT TURN 54..56 FT /evidence="ECO:0007829|PDB:5MSM" FT STRAND 59..62 FT /evidence="ECO:0007829|PDB:5MSM" FT STRAND 65..84 FT /evidence="ECO:0007829|PDB:5MSM" FT TURN 85..87 FT /evidence="ECO:0007829|PDB:5MSM" FT STRAND 88..107 FT /evidence="ECO:0007829|PDB:5MSM" FT TURN 108..111 FT /evidence="ECO:0007829|PDB:5MSM" FT STRAND 112..125 FT /evidence="ECO:0007829|PDB:5MSM" SQ SEQUENCE 133 AA; 15169 MW; D1E4A255B974D51F CRC64; MPSVDIDASQ WQKLTQSREK QTTVITPLGM MMLEIQGELE LPKDFASLAR RDSPNEGRFS EQDGETLIRF GSLQIDGERA TLFVGKKQRL LGKVTKLDVP MGIMHFNSKD NKVELVDVMK YKVIFKDRPL PIM //