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P38877 (CTF8_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 103. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Chromosome transmission fidelity protein 8
Gene names
Name:CTF8
Ordered Locus Names:YHR191C
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length133 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Essential for the fidelity of chromosome transmission. Required for the DNA replication block checkpoint. Component of the RFC-like complex CTF18-RFC which is required for efficient establishment of chromosome cohesion during S-phase and may load or unload POL30/PCNA. During a clamp loading circle, the RFC:clamp complex binds to DNA and the recognition of the double-stranded/single-stranded junction stimulates ATP hydrolysis by RFC. The complex presumably provides bipartite ATP sites in which one subunit supplies a catalytic site for hydrolysis of ATP bound to the neighboring subunit. Dissociation of RFC from the clamp leaves the clamp encircling DNA. Ref.1 Ref.5

Subunit structure

Component of the CTF18-RFC complex, which consists of CTF18, CTF8, DCC1, RFC2, RFC3, RFC4 and RFC5. Ref.1 Ref.5

Subcellular location

Nucleus. Note: Associates with chromatin. Ref.1

Sequence similarities

Belongs to the CTF8 family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 133133Chromosome transmission fidelity protein 8
PRO_0000079494

Sequences

Sequence LengthMass (Da)Tools
P38877 [UniParc].

Last modified February 1, 1995. Version 1.
Checksum: D1E4A255B974D51F

FASTA13315,169
        10         20         30         40         50         60 
MPSVDIDASQ WQKLTQSREK QTTVITPLGM MMLEIQGELE LPKDFASLAR RDSPNEGRFS 

        70         80         90        100        110        120 
EQDGETLIRF GSLQIDGERA TLFVGKKQRL LGKVTKLDVP MGIMHFNSKD NKVELVDVMK 

       130 
YKVIFKDRPL PIM 

« Hide

References

« Hide 'large scale' references
[1]"Identification of RFC(Ctf18p, Ctf8p, Dcc1p): an alternative RFC complex required for sister chromatid cohesion in S. cerevisiae."
Mayer M.L., Gygi S.P., Aebersold R., Hieter P.
Mol. Cell 7:959-970(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, IDENTIFICATION IN THE CTF18-RFC COMPLEX, SUBCELLULAR LOCATION.
[2]"Complete nucleotide sequence of Saccharomyces cerevisiae chromosome VIII."
Johnston M., Andrews S., Brinkman R., Cooper J., Ding H., Dover J., Du Z., Favello A., Fulton L., Gattung S., Geisel C., Kirsten J., Kucaba T., Hillier L.W., Jier M., Johnston L., Langston Y., Latreille P. expand/collapse author list , Louis E.J., Macri C., Mardis E., Menezes S., Mouser L., Nhan M., Rifkin L., Riles L., St Peter H., Trevaskis E., Vaughan K., Vignati D., Wilcox L., Wohldman P., Waterston R., Wilson R., Vaudin M.
Science 265:2077-2082(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[3]"The reference genome sequence of Saccharomyces cerevisiae: Then and now."
Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R., Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S., Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.
G3 (Bethesda) 4:389-398(2014) [PubMed] [Europe PMC] [Abstract]
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[4]"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae."
Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. expand/collapse author list , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J.
Genome Res. 17:536-543(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[5]"Replication protein A-directed unloading of PCNA by the Ctf18 cohesion establishment complex."
Bylund G.O., Burgers P.M.
Mol. Cell. Biol. 25:5445-5455(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION IN THE CTF18-RFC COMPLEX, FUNCTION OF THE CTF18-RFC COMPLEX.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U53671 Genomic DNA. Translation: AAA98983.1.
U00030 Genomic DNA. Translation: AAB68366.1.
AY558287 Genomic DNA. Translation: AAS56613.1.
BK006934 Genomic DNA. Translation: DAA06883.1.
PIRS46688.
RefSeqNP_012061.3. NM_001179322.3.

3D structure databases

ProteinModelPortalP38877.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid36625. 348 interactions.
DIPDIP-2727N.
IntActP38877. 8 interactions.
MINTMINT-657494.
STRING4932.YHR191C.

Proteomic databases

MaxQBP38877.
PaxDbP38877.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYHR191C; YHR191C; YHR191C.
GeneID856598.
KEGGsce:YHR191C.

Organism-specific databases

CYGDYHR191c.
SGDS000001234. CTF8.

Phylogenomic databases

eggNOGNOG127432.
HOGENOMHOG000190820.
KOK11270.
OMAQRPLPIM.
OrthoDBEOG7D868W.

Enzyme and pathway databases

BioCycYEAST:G3O-31219-MONOMER.

Gene expression databases

GenevestigatorP38877.

Family and domain databases

InterProIPR018607. Chromosome_trans_fidel_Ctf8.
[Graphical view]
PfamPF09696. Ctf8. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio982490.

Entry information

Entry nameCTF8_YEAST
AccessionPrimary (citable) accession number: P38877
Secondary accession number(s): D3DLD9
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: February 1, 1995
Last modified: June 11, 2014
This is version 103 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome VIII

Yeast (Saccharomyces cerevisiae) chromosome VIII: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families