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P38877

- CTF8_YEAST

UniProt

P38877 - CTF8_YEAST

Protein

Chromosome transmission fidelity protein 8

Gene

CTF8

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 104 (01 Oct 2014)
      Sequence version 1 (01 Feb 1995)
      Previous versions | rss
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    Functioni

    Essential for the fidelity of chromosome transmission. Required for the DNA replication block checkpoint. Component of the RFC-like complex CTF18-RFC which is required for efficient establishment of chromosome cohesion during S-phase and may load or unload POL30/PCNA. During a clamp loading circle, the RFC:clamp complex binds to DNA and the recognition of the double-stranded/single-stranded junction stimulates ATP hydrolysis by RFC. The complex presumably provides bipartite ATP sites in which one subunit supplies a catalytic site for hydrolysis of ATP bound to the neighboring subunit. Dissociation of RFC from the clamp leaves the clamp encircling DNA.2 Publications

    GO - Molecular functioni

    1. DNA binding Source: UniProtKB-KW
    2. protein binding Source: IntAct

    GO - Biological processi

    1. DNA replication Source: UniProtKB-KW
    2. maintenance of DNA trinucleotide repeats Source: SGD
    3. mitotic sister chromatid cohesion Source: SGD

    Keywords - Biological processi

    Cell cycle, DNA replication

    Keywords - Ligandi

    DNA-binding

    Enzyme and pathway databases

    BioCyciYEAST:G3O-31219-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Chromosome transmission fidelity protein 8
    Gene namesi
    Name:CTF8
    Ordered Locus Names:YHR191C
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome VIII

    Organism-specific databases

    CYGDiYHR191c.
    SGDiS000001234. CTF8.

    Subcellular locationi

    Nucleus 1 Publication
    Note: Associates with chromatin.

    GO - Cellular componenti

    1. Ctf18 RFC-like complex Source: SGD
    2. nucleus Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 133133Chromosome transmission fidelity protein 8PRO_0000079494Add
    BLAST

    Proteomic databases

    MaxQBiP38877.
    PaxDbiP38877.

    Expressioni

    Gene expression databases

    GenevestigatoriP38877.

    Interactioni

    Subunit structurei

    Component of the CTF18-RFC complex, which consists of CTF18, CTF8, DCC1, RFC2, RFC3, RFC4 and RFC5.2 Publications

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    CTF18P499565EBI-5216,EBI-4560
    DCC1P255592EBI-5216,EBI-5661
    RFC2P403482EBI-5216,EBI-14992
    RFC3P386292EBI-5216,EBI-15000
    RFC4P403392EBI-5216,EBI-15009
    RFC5P382512EBI-5216,EBI-15016

    Protein-protein interaction databases

    BioGridi36625. 348 interactions.
    DIPiDIP-2727N.
    IntActiP38877. 8 interactions.
    MINTiMINT-657494.
    STRINGi4932.YHR191C.

    Structurei

    3D structure databases

    ProteinModelPortaliP38877.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the CTF8 family.Curated

    Phylogenomic databases

    eggNOGiNOG127432.
    HOGENOMiHOG000190820.
    KOiK11270.
    OMAiQRPLPIM.
    OrthoDBiEOG7D868W.

    Family and domain databases

    InterProiIPR018607. Chromosome_trans_fidel_Ctf8.
    [Graphical view]
    PfamiPF09696. Ctf8. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P38877-1 [UniParc]FASTAAdd to Basket

    « Hide

    MPSVDIDASQ WQKLTQSREK QTTVITPLGM MMLEIQGELE LPKDFASLAR    50
    RDSPNEGRFS EQDGETLIRF GSLQIDGERA TLFVGKKQRL LGKVTKLDVP 100
    MGIMHFNSKD NKVELVDVMK YKVIFKDRPL PIM 133
    Length:133
    Mass (Da):15,169
    Last modified:February 1, 1995 - v1
    Checksum:iD1E4A255B974D51F
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U53671 Genomic DNA. Translation: AAA98983.1.
    U00030 Genomic DNA. Translation: AAB68366.1.
    AY558287 Genomic DNA. Translation: AAS56613.1.
    BK006934 Genomic DNA. Translation: DAA06883.1.
    PIRiS46688.
    RefSeqiNP_012061.3. NM_001179322.3.

    Genome annotation databases

    EnsemblFungiiYHR191C; YHR191C; YHR191C.
    GeneIDi856598.
    KEGGisce:YHR191C.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U53671 Genomic DNA. Translation: AAA98983.1 .
    U00030 Genomic DNA. Translation: AAB68366.1 .
    AY558287 Genomic DNA. Translation: AAS56613.1 .
    BK006934 Genomic DNA. Translation: DAA06883.1 .
    PIRi S46688.
    RefSeqi NP_012061.3. NM_001179322.3.

    3D structure databases

    ProteinModelPortali P38877.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 36625. 348 interactions.
    DIPi DIP-2727N.
    IntActi P38877. 8 interactions.
    MINTi MINT-657494.
    STRINGi 4932.YHR191C.

    Proteomic databases

    MaxQBi P38877.
    PaxDbi P38877.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YHR191C ; YHR191C ; YHR191C .
    GeneIDi 856598.
    KEGGi sce:YHR191C.

    Organism-specific databases

    CYGDi YHR191c.
    SGDi S000001234. CTF8.

    Phylogenomic databases

    eggNOGi NOG127432.
    HOGENOMi HOG000190820.
    KOi K11270.
    OMAi QRPLPIM.
    OrthoDBi EOG7D868W.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-31219-MONOMER.

    Miscellaneous databases

    NextBioi 982490.

    Gene expression databases

    Genevestigatori P38877.

    Family and domain databases

    InterProi IPR018607. Chromosome_trans_fidel_Ctf8.
    [Graphical view ]
    Pfami PF09696. Ctf8. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Identification of RFC(Ctf18p, Ctf8p, Dcc1p): an alternative RFC complex required for sister chromatid cohesion in S. cerevisiae."
      Mayer M.L., Gygi S.P., Aebersold R., Hieter P.
      Mol. Cell 7:959-970(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, IDENTIFICATION IN THE CTF18-RFC COMPLEX, SUBCELLULAR LOCATION.
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    3. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    4. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    5. "Replication protein A-directed unloading of PCNA by the Ctf18 cohesion establishment complex."
      Bylund G.O., Burgers P.M.
      Mol. Cell. Biol. 25:5445-5455(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION IN THE CTF18-RFC COMPLEX, FUNCTION OF THE CTF18-RFC COMPLEX.

    Entry informationi

    Entry nameiCTF8_YEAST
    AccessioniPrimary (citable) accession number: P38877
    Secondary accession number(s): D3DLD9
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 1, 1995
    Last sequence update: February 1, 1995
    Last modified: October 1, 2014
    This is version 104 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families
    2. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    3. Yeast chromosome VIII
      Yeast (Saccharomyces cerevisiae) chromosome VIII: entries and gene names

    External Data

    Dasty 3