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Protein

Chromosome transmission fidelity protein 8

Gene

CTF8

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Essential for the fidelity of chromosome transmission. Required for the DNA replication block checkpoint. Component of the RFC-like complex CTF18-RFC which is required for efficient establishment of chromosome cohesion during S-phase and may load or unload POL30/PCNA. During a clamp loading circle, the RFC:clamp complex binds to DNA and the recognition of the double-stranded/single-stranded junction stimulates ATP hydrolysis by RFC. The complex presumably provides bipartite ATP sites in which one subunit supplies a catalytic site for hydrolysis of ATP bound to the neighboring subunit. Dissociation of RFC from the clamp leaves the clamp encircling DNA.2 Publications

GO - Molecular functioni

GO - Biological processi

  • DNA replication Source: UniProtKB-KW
  • maintenance of DNA trinucleotide repeats Source: SGD
  • mitotic sister chromatid cohesion Source: SGD
Complete GO annotation...

Keywords - Biological processi

Cell cycle, DNA replication

Keywords - Ligandi

DNA-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-31219-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Chromosome transmission fidelity protein 8
Gene namesi
Name:CTF8
Ordered Locus Names:YHR191C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311 Componenti: Chromosome VIII

Organism-specific databases

CYGDiYHR191c.
EuPathDBiFungiDB:YHR191C.
SGDiS000001234. CTF8.

Subcellular locationi

  • Nucleus 1 Publication

  • Note: Associates with chromatin.

GO - Cellular componenti

  • Ctf18 RFC-like complex Source: SGD
  • nucleus Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 133133Chromosome transmission fidelity protein 8PRO_0000079494Add
BLAST

Proteomic databases

MaxQBiP38877.
PaxDbiP38877.

Interactioni

Subunit structurei

Component of the CTF18-RFC complex, which consists of CTF18, CTF8, DCC1, RFC2, RFC3, RFC4 and RFC5.2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
CTF18P499565EBI-5216,EBI-4560
DCC1P255592EBI-5216,EBI-5661
RFC2P403482EBI-5216,EBI-14992
RFC3P386292EBI-5216,EBI-15000
RFC4P403392EBI-5216,EBI-15009
RFC5P382512EBI-5216,EBI-15016

Protein-protein interaction databases

BioGridi36625. 349 interactions.
DIPiDIP-2727N.
IntActiP38877. 8 interactions.
MINTiMINT-657494.
STRINGi4932.YHR191C.

Structurei

3D structure databases

ProteinModelPortaliP38877.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the CTF8 family.Curated

Phylogenomic databases

eggNOGiNOG127432.
HOGENOMiHOG000190820.
InParanoidiP38877.
KOiK11270.
OMAiQRPLPIM.
OrthoDBiEOG7D868W.

Family and domain databases

InterProiIPR018607. Chromosome_trans_fidel_Ctf8.
[Graphical view]
PfamiPF09696. Ctf8. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P38877-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPSVDIDASQ WQKLTQSREK QTTVITPLGM MMLEIQGELE LPKDFASLAR
60 70 80 90 100
RDSPNEGRFS EQDGETLIRF GSLQIDGERA TLFVGKKQRL LGKVTKLDVP
110 120 130
MGIMHFNSKD NKVELVDVMK YKVIFKDRPL PIM
Length:133
Mass (Da):15,169
Last modified:February 1, 1995 - v1
Checksum:iD1E4A255B974D51F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U53671 Genomic DNA. Translation: AAA98983.1.
U00030 Genomic DNA. Translation: AAB68366.1.
AY558287 Genomic DNA. Translation: AAS56613.1.
BK006934 Genomic DNA. Translation: DAA06883.1.
PIRiS46688.
RefSeqiNP_012061.3. NM_001179322.3.

Genome annotation databases

EnsemblFungiiYHR191C; YHR191C; YHR191C.
GeneIDi856598.
KEGGisce:YHR191C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U53671 Genomic DNA. Translation: AAA98983.1.
U00030 Genomic DNA. Translation: AAB68366.1.
AY558287 Genomic DNA. Translation: AAS56613.1.
BK006934 Genomic DNA. Translation: DAA06883.1.
PIRiS46688.
RefSeqiNP_012061.3. NM_001179322.3.

3D structure databases

ProteinModelPortaliP38877.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi36625. 349 interactions.
DIPiDIP-2727N.
IntActiP38877. 8 interactions.
MINTiMINT-657494.
STRINGi4932.YHR191C.

Proteomic databases

MaxQBiP38877.
PaxDbiP38877.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYHR191C; YHR191C; YHR191C.
GeneIDi856598.
KEGGisce:YHR191C.

Organism-specific databases

CYGDiYHR191c.
EuPathDBiFungiDB:YHR191C.
SGDiS000001234. CTF8.

Phylogenomic databases

eggNOGiNOG127432.
HOGENOMiHOG000190820.
InParanoidiP38877.
KOiK11270.
OMAiQRPLPIM.
OrthoDBiEOG7D868W.

Enzyme and pathway databases

BioCyciYEAST:G3O-31219-MONOMER.

Miscellaneous databases

NextBioi982490.
PROiP38877.

Family and domain databases

InterProiIPR018607. Chromosome_trans_fidel_Ctf8.
[Graphical view]
PfamiPF09696. Ctf8. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Identification of RFC(Ctf18p, Ctf8p, Dcc1p): an alternative RFC complex required for sister chromatid cohesion in S. cerevisiae."
    Mayer M.L., Gygi S.P., Aebersold R., Hieter P.
    Mol. Cell 7:959-970(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, IDENTIFICATION IN THE CTF18-RFC COMPLEX, SUBCELLULAR LOCATION.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  4. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  5. "Replication protein A-directed unloading of PCNA by the Ctf18 cohesion establishment complex."
    Bylund G.O., Burgers P.M.
    Mol. Cell. Biol. 25:5445-5455(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION IN THE CTF18-RFC COMPLEX, FUNCTION OF THE CTF18-RFC COMPLEX.

Entry informationi

Entry nameiCTF8_YEAST
AccessioniPrimary (citable) accession number: P38877
Secondary accession number(s): D3DLD9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: February 1, 1995
Last modified: June 24, 2015
This is version 108 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome VIII
    Yeast (Saccharomyces cerevisiae) chromosome VIII: entries and gene names

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.