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Protein

Chromosome transmission fidelity protein 8

Gene

CTF8

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Essential for the fidelity of chromosome transmission. Required for the DNA replication block checkpoint. Component of the RFC-like complex CTF18-RFC which is required for efficient establishment of chromosome cohesion during S-phase and may load or unload POL30/PCNA. During a clamp loading circle, the RFC:clamp complex binds to DNA and the recognition of the double-stranded/single-stranded junction stimulates ATP hydrolysis by RFC. The complex presumably provides bipartite ATP sites in which one subunit supplies a catalytic site for hydrolysis of ATP bound to the neighboring subunit. Dissociation of RFC from the clamp leaves the clamp encircling DNA.2 Publications

GO - Molecular functioni

GO - Biological processi

  • DNA replication Source: UniProtKB-KW
  • maintenance of DNA trinucleotide repeats Source: SGD
  • mitotic sister chromatid cohesion Source: SGD

Keywordsi

Molecular functionDNA-binding
Biological processCell cycle, DNA replication

Enzyme and pathway databases

BioCyciYEAST:G3O-31219-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Chromosome transmission fidelity protein 8
Gene namesi
Name:CTF8
Ordered Locus Names:YHR191C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome VIII

Organism-specific databases

EuPathDBiFungiDB:YHR191C.
SGDiS000001234. CTF8.

Subcellular locationi

  • Nucleus 1 Publication

  • Note: Associates with chromatin.

GO - Cellular componenti

  • Ctf18 RFC-like complex Source: SGD
  • nucleus Source: UniProtKB-SubCell

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000794941 – 133Chromosome transmission fidelity protein 8Add BLAST133

Proteomic databases

MaxQBiP38877.
PRIDEiP38877.

Interactioni

Subunit structurei

Component of the CTF18-RFC complex, which consists of CTF18, CTF8, DCC1, RFC2, RFC3, RFC4 and RFC5.2 Publications

Binary interactionsi

Show more details

Protein-protein interaction databases

BioGridi36625. 622 interactors.
DIPiDIP-2727N.
IntActiP38877. 8 interactors.
MINTiMINT-657494.

Structurei

3D structure databases

ProteinModelPortaliP38877.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the CTF8 family.Curated

Phylogenomic databases

HOGENOMiHOG000190820.
InParanoidiP38877.
KOiK11270.
OMAiQRPLPIM.
OrthoDBiEOG092C5S53.

Family and domain databases

InterProiView protein in InterPro
IPR018607. Ctf8.
PfamiView protein in Pfam
PF09696. Ctf8. 1 hit.

Sequencei

Sequence statusi: Complete.

P38877-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPSVDIDASQ WQKLTQSREK QTTVITPLGM MMLEIQGELE LPKDFASLAR
60 70 80 90 100
RDSPNEGRFS EQDGETLIRF GSLQIDGERA TLFVGKKQRL LGKVTKLDVP
110 120 130
MGIMHFNSKD NKVELVDVMK YKVIFKDRPL PIM
Length:133
Mass (Da):15,169
Last modified:February 1, 1995 - v1
Checksum:iD1E4A255B974D51F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U53671 Genomic DNA. Translation: AAA98983.1.
U00030 Genomic DNA. Translation: AAB68366.1.
AY558287 Genomic DNA. Translation: AAS56613.1.
BK006934 Genomic DNA. Translation: DAA06883.1.
PIRiS46688.
RefSeqiNP_012061.3. NM_001179322.3.

Genome annotation databases

EnsemblFungiiYHR191C; YHR191C; YHR191C.
GeneIDi856598.
KEGGisce:YHR191C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U53671 Genomic DNA. Translation: AAA98983.1.
U00030 Genomic DNA. Translation: AAB68366.1.
AY558287 Genomic DNA. Translation: AAS56613.1.
BK006934 Genomic DNA. Translation: DAA06883.1.
PIRiS46688.
RefSeqiNP_012061.3. NM_001179322.3.

3D structure databases

ProteinModelPortaliP38877.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi36625. 622 interactors.
DIPiDIP-2727N.
IntActiP38877. 8 interactors.
MINTiMINT-657494.

Proteomic databases

MaxQBiP38877.
PRIDEiP38877.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYHR191C; YHR191C; YHR191C.
GeneIDi856598.
KEGGisce:YHR191C.

Organism-specific databases

EuPathDBiFungiDB:YHR191C.
SGDiS000001234. CTF8.

Phylogenomic databases

HOGENOMiHOG000190820.
InParanoidiP38877.
KOiK11270.
OMAiQRPLPIM.
OrthoDBiEOG092C5S53.

Enzyme and pathway databases

BioCyciYEAST:G3O-31219-MONOMER.

Miscellaneous databases

PROiP38877.

Family and domain databases

InterProiView protein in InterPro
IPR018607. Ctf8.
PfamiView protein in Pfam
PF09696. Ctf8. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiCTF8_YEAST
AccessioniPrimary (citable) accession number: P38877
Secondary accession number(s): D3DLD9
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: February 1, 1995
Last modified: March 15, 2017
This is version 122 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome VIII
    Yeast (Saccharomyces cerevisiae) chromosome VIII: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.