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Protein

Chromosome transmission fidelity protein 8

Gene

CTF8

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Essential for the fidelity of chromosome transmission. Required for the DNA replication block checkpoint. Component of the RFC-like complex CTF18-RFC which is required for efficient establishment of chromosome cohesion during S-phase and may load or unload POL30/PCNA. During a clamp loading circle, the RFC:clamp complex binds to DNA and the recognition of the double-stranded/single-stranded junction stimulates ATP hydrolysis by RFC. The complex presumably provides bipartite ATP sites in which one subunit supplies a catalytic site for hydrolysis of ATP bound to the neighboring subunit. Dissociation of RFC from the clamp leaves the clamp encircling DNA.2 Publications

GO - Molecular functioni

GO - Biological processi

  • DNA replication Source: UniProtKB-KW
  • maintenance of DNA trinucleotide repeats Source: SGD
  • mitotic sister chromatid cohesion Source: SGD

Keywordsi

Molecular functionDNA-binding
Biological processCell cycle, DNA replication

Enzyme and pathway databases

BioCyciYEAST:G3O-31219-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Chromosome transmission fidelity protein 8
Gene namesi
Name:CTF8
Ordered Locus Names:YHR191C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome VIII

Organism-specific databases

EuPathDBiFungiDB:YHR191C
SGDiS000001234 CTF8

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000794941 – 133Chromosome transmission fidelity protein 8Add BLAST133

Proteomic databases

MaxQBiP38877
PaxDbiP38877
PRIDEiP38877

Interactioni

Subunit structurei

Component of the CTF18-RFC complex, which consists of CTF18, CTF8, DCC1, RFC2, RFC3, RFC4 and RFC5.2 Publications

Binary interactionsi

Show more details

Protein-protein interaction databases

BioGridi36625, 625 interactors
DIPiDIP-2727N
IntActiP38877, 8 interactors
MINTiP38877
STRINGi4932.YHR191C

Structurei

Secondary structure

1133
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi3 – 7Combined sources5
Helixi9 – 14Combined sources6
Beta strandi22 – 25Combined sources4
Beta strandi29 – 40Combined sources12
Helixi45 – 50Combined sources6
Turni54 – 56Combined sources3
Beta strandi59 – 62Combined sources4
Beta strandi65 – 84Combined sources20
Turni85 – 87Combined sources3
Beta strandi88 – 107Combined sources20
Turni108 – 111Combined sources4
Beta strandi112 – 125Combined sources14

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
5MSMX-ray2.29B/E1-133[»]
5OKCX-ray2.30F/H1-133[»]
5OKIX-ray4.50D/H1-133[»]
ProteinModelPortaliP38877
SMRiP38877
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the CTF8 family.Curated

Phylogenomic databases

HOGENOMiHOG000190820
InParanoidiP38877
KOiK11270
OMAiGQHQRLT
OrthoDBiEOG092C5S53

Family and domain databases

InterProiView protein in InterPro
IPR018607 Ctf8
PfamiView protein in Pfam
PF09696 Ctf8, 1 hit

Sequencei

Sequence statusi: Complete.

P38877-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPSVDIDASQ WQKLTQSREK QTTVITPLGM MMLEIQGELE LPKDFASLAR
60 70 80 90 100
RDSPNEGRFS EQDGETLIRF GSLQIDGERA TLFVGKKQRL LGKVTKLDVP
110 120 130
MGIMHFNSKD NKVELVDVMK YKVIFKDRPL PIM
Length:133
Mass (Da):15,169
Last modified:February 1, 1995 - v1
Checksum:iD1E4A255B974D51F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U53671 Genomic DNA Translation: AAA98983.1
U00030 Genomic DNA Translation: AAB68366.1
AY558287 Genomic DNA Translation: AAS56613.1
BK006934 Genomic DNA Translation: DAA06883.1
PIRiS46688
RefSeqiNP_012061.3, NM_001179322.3

Genome annotation databases

EnsemblFungiiYHR191C; YHR191C; YHR191C
GeneIDi856598
KEGGisce:YHR191C

Similar proteinsi

Entry informationi

Entry nameiCTF8_YEAST
AccessioniPrimary (citable) accession number: P38877
Secondary accession number(s): D3DLD9
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: February 1, 1995
Last modified: May 23, 2018
This is version 134 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome VIII
    Yeast (Saccharomyces cerevisiae) chromosome VIII: entries and gene names

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