P38877 (CTF8_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Chromosome transmission fidelity protein 8

Gene names

Name:	CTF8
Ordered Locus Names:	YHR191C

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › Saccharomyces cerevisiae

Protein attributes

Sequence length	133 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Essential for the fidelity of chromosome transmission. Required for the DNA replication block checkpoint. Component of the RFC-like complex CTF18-RFC which is required for efficient establishment of chromosome cohesion during S-phase and may load or unload POL30/PCNA. During a clamp loading circle, the RFC:clamp complex binds to DNA and the recognition of the double-stranded/single-stranded junction stimulates ATP hydrolysis by RFC. The complex presumably provides bipartite ATP sites in which one subunit supplies a catalytic site for hydrolysis of ATP bound to the neighboring subunit. Dissociation of RFC from the clamp leaves the clamp encircling DNA. Ref.1 Ref.5
Subunit structure	Component of the CTF18-RFC complex, which consists of CTF18, CTF8, DCC1, RFC2, RFC3, RFC4 and RFC5. Ref.1 Ref.5
Subcellular location	Nucleus. Note: Associates with chromatin. Ref.1
Sequence similarities	Belongs to the CTF8 family.

Ontologies

Keywords
Biological process	Cell cycle DNA replication
Cellular component	Nucleus
Ligand	DNA-binding
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	DNA replication Inferred from electronic annotation. Source: UniProtKB-KW maintenance of DNA trinucleotide repeats Inferred from mutant phenotype PubMed 21347277. Source: SGD mitotic sister chromatid cohesion Inferred from mutant phenotype Ref.1. Source: SGD
Cellular_component	Ctf18 RFC-like complex Inferred from physical interaction Ref.1. Source: SGD nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	DNA binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
CTF18	P49956	5	EBI-5216,EBI-4560
DCC1	P25559	2	EBI-5216,EBI-5661
RFC2	P40348	2	EBI-5216,EBI-14992
RFC3	P38629	2	EBI-5216,EBI-15000
RFC4	P40339	2	EBI-5216,EBI-15009
RFC5	P38251	2	EBI-5216,EBI-15016

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 133	133	Chromosome transmission fidelity protein 8		PRO_0000079494

Sequences

	Sequence		Length	Mass (Da)
	P38877 [UniParc]. Last modified February 1, 1995. Version 1. Checksum: D1E4A255B974D51F	FASTA	133	15,169
	10 20 30 40 50 60 MPSVDIDASQ WQKLTQSREK QTTVITPLGM MMLEIQGELE LPKDFASLAR RDSPNEGRFS 70 80 90 100 110 120 EQDGETLIRF GSLQIDGERA TLFVGKKQRL LGKVTKLDVP MGIMHFNSKD NKVELVDVMK 130 YKVIFKDRPL PIM

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Identification of RFC(Ctf18p, Ctf8p, Dcc1p): an alternative RFC complex required for sister chromatid cohesion in S. cerevisiae." Mayer M.L., Gygi S.P., Aebersold R., Hieter P. Mol. Cell 7:959-970(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, IDENTIFICATION IN THE CTF18-RFC COMPLEX, SUBCELLULAR LOCATION.
[2]	"Complete nucleotide sequence of Saccharomyces cerevisiae chromosome VIII." Johnston M., Andrews S., Brinkman R., Cooper J., Ding H., Dover J., Du Z., Favello A., Fulton L., Gattung S., Geisel C., Kirsten J., Kucaba T., Hillier L.W., Jier M., Johnston L., Langston Y., Latreille P. , Louis E.J., Macri C., Mardis E., Menezes S., Mouser L., Nhan M., Rifkin L., Riles L., St Peter H., Trevaskis E., Vaughan K., Vignati D., Wilcox L., Wohldman P., Waterston R., Wilson R., Vaudin M. Science 265:2077-2082(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[3]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[4]	"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae." Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J. Genome Res. 17:536-543(2007) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[5]	"Replication protein A-directed unloading of PCNA by the Ctf18 cohesion establishment complex." Bylund G.O., Burgers P.M. Mol. Cell. Biol. 25:5445-5455(2005) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION IN THE CTF18-RFC COMPLEX, FUNCTION OF THE CTF18-RFC COMPLEX.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U53671 Genomic DNA. Translation: AAA98983.1. U00030 Genomic DNA. Translation: AAB68366.1. AY558287 Genomic DNA. Translation: AAS56613.1. BK006934 Genomic DNA. Translation: DAA06883.1.
PIR	S46688.
RefSeq	NP_012061.3. NM_001179322.3.
3D structure databases
ProteinModelPortal	P38877.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-2727N.
IntAct	P38877. 8 interactions.
MINT	MINT-657494.
STRING	4932.YHR191C.
Proteomic databases
PaxDb	P38877.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YHR191C; YHR191C; YHR191C.
GeneID	856598.
KEGG	sce:YHR191C.
Organism-specific databases
CYGD	YHR191c.
SGD	S000001234. CTF8.
Phylogenomic databases
eggNOG	NOG127432.
HOGENOM	HOG000190820.
KO	K11270.
OMA	IEIQGDL.
OrthoDB	EOG4WDHN8.
Enzyme and pathway databases
BioCyc	YEAST:G3O-31219-MONOMER.
Gene expression databases
Genevestigator	P38877.
GermOnline	YHR191C. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR018607. Chromosome_trans_fidel_Ctf8. [Graphical view]
Pfam	PF09696. Ctf8. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	982490.

Entry information

Entry name

CTF8_YEAST

Accession

Primary (citable) accession number: P38877
Secondary accession number(s): D3DLD9

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1995
Last sequence update:	February 1, 1995
Last modified:	May 29, 2013
This is version 96 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program