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P38874 (ELP5_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 114. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Elongator complex protein 5
Alternative name(s):
Gamma-toxin target 5
HAT-associated protein 2
Protein IKI1
Gene names
Name:IKI1
Synonyms:ELP5, HAP2, TOT5
Ordered Locus Names:YHR187W
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length309 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Acts as component of the RNA polymerase II elongator complex, which is a major histone acetyltransferase component of the RNA polymerase II (RNAPII) holoenzyme and is involved in transcriptional elongation. Association with elongating RNAPII requires a hyperphosphorylated state of the RNAPII C-terminal domain (CTD). Elongator binds to both naked and nucleosomal DNA, can acetylate both core and nucleosomal histones, and is involved in chromatin remodeling. It acetylates histones H3, preferentially at 'Lys-14', and H4, preferentially at 'Lys-8'. It functions as a gamma-toxin target (TOT); disruption of the complex confers resistance to Kluyveromyces lactis toxin zymocin (pGKL1 killer toxin). May also be involved in sensitiviy to Pichia inositovora toxin. May be involved in tRNA modification. IKI1 is required for an early step in synthesis of 5-methoxycarbonylmethyl (mcm5) and 5-carbamoylmethyl (ncm5) groups present on uridines at the wobble position in tRNA. Ref.4 Ref.5 Ref.8 Ref.9 Ref.10 Ref.14

Subunit structure

Component of the RNA polymerase II elongator complex, which consists of IKI3, ELP2, ELP3, ELP4, IKI1 and ELP6. ELP4, IKI1 and ELP6 form a distinct subcomplex (HAP). Ref.6 Ref.7 Ref.8

Subcellular location

Cytoplasm. Nucleus Ref.4 Ref.11 Ref.13.

Miscellaneous

Present with 3870 molecules/cell in log phase SD medium.

Sequence similarities

Belongs to the ELP5 family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 309309Elongator complex protein 5
PRO_0000084178

Secondary structure

.................................... 309
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P38874 [UniParc].

Last modified February 1, 1995. Version 1.
Checksum: DFD93D7BA583E2F5

FASTA30935,220
        10         20         30         40         50         60 
MASSSHNPVI LLKRILSLTE SSPFILCLDS IAQTSYKLIQ EFVHQSKSKG NEYPIVYISF 

        70         80         90        100        110        120 
ETVNKPSYCT QFIDATQMDF VHLVKQIISY LPAATATQAK KHMVIIDSLN YISTEYITRF 

       130        140        150        160        170        180 
LSEIASPHCT MVATYHKDIK DENRTVIPDW NNNYPDKLTL LQFMATTIVD IDVVLTGTLD 

       190        200        210        220        230        240 
TEEVSELLNE FRIPRGLNND IFQLRLVNKR KSGRSLEYDF IVNSNTHEYE LLSTTKQEEE 

       250        260        270        280        290        300 
SSSNGLETPE MLQGLTTFNL GTSNKQKLAK DQVALPFLEA QSFGQGGAIV YEYEKDDDYD 


EEDPYEDPF 

« Hide

References

« Hide 'large scale' references
[1]"Characterization of IKI1 and IKI3 genes conferring pGKL killer sensitivity on Saccharomyces cerevisiae."
Yajima H., Tokunaga M., Nakayama-Murayama A., Hishinuma F.
Biosci. Biotechnol. Biochem. 61:704-709(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"Complete nucleotide sequence of Saccharomyces cerevisiae chromosome VIII."
Johnston M., Andrews S., Brinkman R., Cooper J., Ding H., Dover J., Du Z., Favello A., Fulton L., Gattung S., Geisel C., Kirsten J., Kucaba T., Hillier L.W., Jier M., Johnston L., Langston Y., Latreille P. expand/collapse author list , Louis E.J., Macri C., Mardis E., Menezes S., Mouser L., Nhan M., Rifkin L., Riles L., St Peter H., Trevaskis E., Vaughan K., Vignati D., Wilcox L., Wohldman P., Waterston R., Wilson R., Vaudin M.
Science 265:2077-2082(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[3]"The reference genome sequence of Saccharomyces cerevisiae: Then and now."
Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R., Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S., Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.
G3 (Bethesda) 4:389-398(2014) [PubMed] [Europe PMC] [Abstract]
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[4]"Elongator, a multisubunit component of a novel RNA polymerase II holoenzyme for transcriptional elongation."
Otero G., Fellows J., Li Y., de Bizemont T., Dirac A.M., Gustafsson C.M., Erdjument-Bromage H., Tempst P., Svejstrup J.Q.
Mol. Cell 3:109-118(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION OF THE RNA POLYMERASE II ELONGATOR COMPLEX IN TRANSCRIPTIONAL REGULATION, SUBCELLULAR LOCATION.
[5]"Saccharomyces cerevisiae Elongator mutations confer resistance to the Kluyveromyces lactis zymocin."
Frohloff F., Fichtner L., Jablonowski D., Breunig K.D., Schaffrath R.
EMBO J. 20:1993-2003(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION OF THE RNA POLYMERASE II ELONGATOR COMPLEX IN ZYMOCIN SENSITIVITY.
[6]"RNA polymerase II elongator holoenzyme is composed of two discrete subcomplexes."
Winkler G.S., Petrakis T.G., Ethelberg S., Tokunaga M., Erdjument-Bromage H., Tempst P., Svejstrup J.Q.
J. Biol. Chem. 276:32743-32749(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION IN THE RNA POLYMERASE II ELONGATOR COMPLEX.
[7]"A multiprotein complex that interacts with RNA polymerase II elongator."
Li Y., Takagi Y., Jiang Y., Tokunaga M., Erdjument-Bromage H., Tempst P., Kornberg R.D.
J. Biol. Chem. 276:29628-29631(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION IN THE RNA POLYMERASE II ELONGATOR COMPLEX, IDENTIFICATION BY MASS SPECTROMETRY.
[8]"Characterization of a six-subunit holo-elongator complex required for the regulated expression of a group of genes in Saccharomyces cerevisiae."
Krogan N.J., Greenblatt J.F.
Mol. Cell. Biol. 21:8203-8212(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION IN THE RNA POLYMERASE II ELONGATOR COMPLEX, FUNCTION OF THE RNA POLYMERASE II ELONGATOR COMPLEX IN TRANSCRIPTIONAL REGULATION.
[9]"Elongator is a histone H3 and H4 acetyltransferase important for normal histone acetylation levels in vivo."
Winkler G.S., Kristjuhan A., Erdjument-Bromage H., Tempst P., Svejstrup J.Q.
Proc. Natl. Acad. Sci. U.S.A. 99:3517-3522(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION OF THE RNA POLYMERASE II ELONGATOR COMPLEX IN HISTONE ACETYLATION.
[10]"Structural and functional analysis of the killer element pPin1-3 from Pichia inositovora."
Klassen R., Meinhardt F.
Mol. Genet. Genomics 270:190-199(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION OF THE RNA POLYMERASE II ELONGATOR COMPLEX IN PICHIA INOSITOVORA TOXIN SENSITIVITY.
[11]"Global analysis of protein localization in budding yeast."
Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K.
Nature 425:686-691(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[12]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[13]"Elp1p, the yeast homolog of the FD disease syndrome protein, negatively regulates exocytosis independently of transcriptional elongation."
Rahl P.B., Chen C.Z., Collins R.N.
Mol. Cell 17:841-853(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION.
[14]"An early step in wobble uridine tRNA modification requires the Elongator complex."
Huang B., Johansson M.J.O., Bystroem A.S.
RNA 11:424-436(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION IN TRNA MODIFICATION.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
D83267 Genomic DNA. Translation: BAA20087.1.
U00030 Genomic DNA. Translation: AAB68362.1.
BK006934 Genomic DNA. Translation: DAA06879.1.
PIRS46684.
RefSeqNP_012057.3. NM_001179318.3.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
4A8JX-ray2.10B/E1-270[»]
4EJSX-ray2.61B1-238[»]
ProteinModelPortalP38874.
SMRP38874. Positions 7-234.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid36621. 118 interactions.
DIPDIP-1967N.
IntActP38874. 9 interactions.
MINTMINT-385467.
STRING4932.YHR187W.

Proteomic databases

MaxQBP38874.
PaxDbP38874.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYHR187W; YHR187W; YHR187W.
GeneID856594.
KEGGsce:YHR187W.

Organism-specific databases

CYGDYHR187w.
SGDS000001230. IKI1.

Phylogenomic databases

eggNOGNOG72129.
HOGENOMHOG000248152.
KOK11376.
OMATNIIYLS.
OrthoDBEOG7XWQ03.

Enzyme and pathway databases

BioCycYEAST:G3O-31217-MONOMER.

Gene expression databases

GenevestigatorP38874.

Family and domain databases

InterProIPR019519. Elp5.
[Graphical view]
PfamPF10483. Elong_Iki1. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio982478.
PROP38874.

Entry information

Entry nameELP5_YEAST
AccessionPrimary (citable) accession number: P38874
Secondary accession number(s): D3DLD5
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: February 1, 1995
Last modified: June 11, 2014
This is version 114 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome VIII

Yeast (Saccharomyces cerevisiae) chromosome VIII: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references