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Protein

Elongator complex protein 5

Gene

IKI1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Acts as component of the RNA polymerase II elongator complex, which is a major histone acetyltransferase component of the RNA polymerase II (RNAPII) holoenzyme and is involved in transcriptional elongation. Association with elongating RNAPII requires a hyperphosphorylated state of the RNAPII C-terminal domain (CTD). Elongator binds to both naked and nucleosomal DNA, can acetylate both core and nucleosomal histones, and is involved in chromatin remodeling. It acetylates histones H3, preferentially at 'Lys-14', and H4, preferentially at 'Lys-8'. It functions as a gamma-toxin target (TOT); disruption of the complex confers resistance to Kluyveromyces lactis toxin zymocin (pGKL1 killer toxin). May also be involved in sensitiviy to Pichia inositovora toxin. May be involved in tRNA modification. IKI1 is required for an early step in synthesis of 5-methoxycarbonylmethyl (mcm5) and 5-carbamoylmethyl (ncm5) groups present on uridines at the wobble position in tRNA.6 Publications

GO - Biological processi

  • regulation of transcription, DNA-templated Source: UniProtKB-KW
  • transcription, DNA-templated Source: UniProtKB-KW
  • tRNA modification Source: SGD
Complete GO annotation...

Keywords - Biological processi

Transcription, Transcription regulation

Enzyme and pathway databases

BioCyciYEAST:G3O-31217-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Elongator complex protein 5
Alternative name(s):
Gamma-toxin target 5
HAT-associated protein 2
Protein IKI1
Gene namesi
Name:IKI1
Synonyms:ELP5, HAP2, TOT5
Ordered Locus Names:YHR187W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome VIII

Organism-specific databases

EuPathDBiFungiDB:YHR187W.
SGDiS000001230. IKI1.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: SGD
  • Elongator holoenzyme complex Source: SGD
  • nucleoplasm Source: Reactome
  • nucleus Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000841781 – 309Elongator complex protein 5Add BLAST309

Proteomic databases

MaxQBiP38874.
PRIDEiP38874.

Interactioni

Subunit structurei

Component of the RNA polymerase II elongator complex, which consists of IKI3, ELP2, ELP3, ELP4, IKI1 and ELP6. ELP4, IKI1 and ELP6 form a distinct subcomplex (HAP).3 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
ELP2P429356EBI-9061,EBI-23459
ELP3Q029086EBI-9061,EBI-33957
ELP4Q0288410EBI-9061,EBI-35277
ELP6Q048686EBI-9061,EBI-27653
IKI3Q067068EBI-9061,EBI-9068

Protein-protein interaction databases

BioGridi36621. 119 interactors.
DIPiDIP-1967N.
IntActiP38874. 10 interactors.
MINTiMINT-385467.

Structurei

Secondary structure

1309
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi8 – 16Combined sources9
Beta strandi23 – 30Combined sources8
Helixi36 – 48Combined sources13
Beta strandi55 – 61Combined sources7
Beta strandi70 – 74Combined sources5
Helixi80 – 89Combined sources10
Beta strandi102 – 107Combined sources6
Helixi109 – 111Combined sources3
Helixi114 – 116Combined sources3
Helixi117 – 124Combined sources8
Beta strandi129 – 136Combined sources8
Turni151 – 153Combined sources3
Helixi157 – 164Combined sources8
Beta strandi166 – 173Combined sources8
Helixi181 – 189Combined sources9
Beta strandi200 – 209Combined sources10
Beta strandi215 – 223Combined sources9
Turni224 – 227Combined sources4
Beta strandi228 – 231Combined sources4

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4A8JX-ray2.10B/E1-270[»]
4EJSX-ray2.61B1-238[»]
ProteinModelPortaliP38874.
SMRiP38874.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the ELP5 family.Curated

Phylogenomic databases

HOGENOMiHOG000248152.
InParanoidiP38874.
KOiK11376.
OMAiYEYEKDD.
OrthoDBiEOG092C3T25.

Family and domain databases

InterProiIPR019519. Elp5.
[Graphical view]
PANTHERiPTHR15641. PTHR15641. 1 hit.
PfamiPF10483. Elong_Iki1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P38874-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MASSSHNPVI LLKRILSLTE SSPFILCLDS IAQTSYKLIQ EFVHQSKSKG
60 70 80 90 100
NEYPIVYISF ETVNKPSYCT QFIDATQMDF VHLVKQIISY LPAATATQAK
110 120 130 140 150
KHMVIIDSLN YISTEYITRF LSEIASPHCT MVATYHKDIK DENRTVIPDW
160 170 180 190 200
NNNYPDKLTL LQFMATTIVD IDVVLTGTLD TEEVSELLNE FRIPRGLNND
210 220 230 240 250
IFQLRLVNKR KSGRSLEYDF IVNSNTHEYE LLSTTKQEEE SSSNGLETPE
260 270 280 290 300
MLQGLTTFNL GTSNKQKLAK DQVALPFLEA QSFGQGGAIV YEYEKDDDYD

EEDPYEDPF
Length:309
Mass (Da):35,220
Last modified:February 1, 1995 - v1
Checksum:iDFD93D7BA583E2F5
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D83267 Genomic DNA. Translation: BAA20087.1.
U00030 Genomic DNA. Translation: AAB68362.1.
BK006934 Genomic DNA. Translation: DAA06879.1.
PIRiS46684.
RefSeqiNP_012057.3. NM_001179318.3.

Genome annotation databases

EnsemblFungiiYHR187W; YHR187W; YHR187W.
GeneIDi856594.
KEGGisce:YHR187W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D83267 Genomic DNA. Translation: BAA20087.1.
U00030 Genomic DNA. Translation: AAB68362.1.
BK006934 Genomic DNA. Translation: DAA06879.1.
PIRiS46684.
RefSeqiNP_012057.3. NM_001179318.3.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4A8JX-ray2.10B/E1-270[»]
4EJSX-ray2.61B1-238[»]
ProteinModelPortaliP38874.
SMRiP38874.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi36621. 119 interactors.
DIPiDIP-1967N.
IntActiP38874. 10 interactors.
MINTiMINT-385467.

Proteomic databases

MaxQBiP38874.
PRIDEiP38874.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYHR187W; YHR187W; YHR187W.
GeneIDi856594.
KEGGisce:YHR187W.

Organism-specific databases

EuPathDBiFungiDB:YHR187W.
SGDiS000001230. IKI1.

Phylogenomic databases

HOGENOMiHOG000248152.
InParanoidiP38874.
KOiK11376.
OMAiYEYEKDD.
OrthoDBiEOG092C3T25.

Enzyme and pathway databases

BioCyciYEAST:G3O-31217-MONOMER.

Miscellaneous databases

PROiP38874.

Family and domain databases

InterProiIPR019519. Elp5.
[Graphical view]
PANTHERiPTHR15641. PTHR15641. 1 hit.
PfamiPF10483. Elong_Iki1. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiELP5_YEAST
AccessioniPrimary (citable) accession number: P38874
Secondary accession number(s): D3DLD5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: February 1, 1995
Last modified: November 2, 2016
This is version 131 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 3870 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome VIII
    Yeast (Saccharomyces cerevisiae) chromosome VIII: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.