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P38874

- ELP5_YEAST

UniProt

P38874 - ELP5_YEAST

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Protein

Elongator complex protein 5

Gene
IKI1, ELP5, HAP2, TOT5, YHR187W
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Acts as component of the RNA polymerase II elongator complex, which is a major histone acetyltransferase component of the RNA polymerase II (RNAPII) holoenzyme and is involved in transcriptional elongation. Association with elongating RNAPII requires a hyperphosphorylated state of the RNAPII C-terminal domain (CTD). Elongator binds to both naked and nucleosomal DNA, can acetylate both core and nucleosomal histones, and is involved in chromatin remodeling. It acetylates histones H3, preferentially at 'Lys-14', and H4, preferentially at 'Lys-8'. It functions as a gamma-toxin target (TOT); disruption of the complex confers resistance to Kluyveromyces lactis toxin zymocin (pGKL1 killer toxin). May also be involved in sensitiviy to Pichia inositovora toxin. May be involved in tRNA modification. IKI1 is required for an early step in synthesis of 5-methoxycarbonylmethyl (mcm5) and 5-carbamoylmethyl (ncm5) groups present on uridines at the wobble position in tRNA.6 Publications

GO - Molecular functioni

  1. protein binding Source: IntAct

GO - Biological processi

  1. ATP catabolic process Source: GOC
  2. regulation of transcription, DNA-templated Source: UniProtKB-KW
  3. transcription, DNA-templated Source: UniProtKB-KW
  4. tRNA modification Source: SGD
Complete GO annotation...

Keywords - Biological processi

Transcription, Transcription regulation

Enzyme and pathway databases

BioCyciYEAST:G3O-31217-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Elongator complex protein 5
Alternative name(s):
Gamma-toxin target 5
HAT-associated protein 2
Protein IKI1
Gene namesi
Name:IKI1
Synonyms:ELP5, HAP2, TOT5
Ordered Locus Names:YHR187W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome VIII

Organism-specific databases

CYGDiYHR187w.
SGDiS000001230. IKI1.

Subcellular locationi

Cytoplasm. Nucleus 3 Publications

GO - Cellular componenti

  1. cytoplasm Source: SGD
  2. Elongator holoenzyme complex Source: SGD
  3. nucleoplasm Source: Reactome
  4. nucleus Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 309309Elongator complex protein 5PRO_0000084178Add
BLAST

Proteomic databases

MaxQBiP38874.
PaxDbiP38874.

Expressioni

Gene expression databases

GenevestigatoriP38874.

Interactioni

Subunit structurei

Component of the RNA polymerase II elongator complex, which consists of IKI3, ELP2, ELP3, ELP4, IKI1 and ELP6. ELP4, IKI1 and ELP6 form a distinct subcomplex (HAP).3 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
ELP2P429356EBI-9061,EBI-23459
ELP3Q029086EBI-9061,EBI-33957
ELP4Q0288410EBI-9061,EBI-35277
ELP6Q048686EBI-9061,EBI-27653
IKI3Q067068EBI-9061,EBI-9068

Protein-protein interaction databases

BioGridi36621. 118 interactions.
DIPiDIP-1967N.
IntActiP38874. 9 interactions.
MINTiMINT-385467.
STRINGi4932.YHR187W.

Structurei

Secondary structure

1
309
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi8 – 169
Beta strandi23 – 308
Helixi36 – 4813
Beta strandi55 – 617
Beta strandi70 – 745
Helixi80 – 8910
Beta strandi102 – 1076
Helixi109 – 1113
Helixi114 – 1163
Helixi117 – 1248
Beta strandi129 – 1368
Turni151 – 1533
Helixi157 – 1648
Beta strandi166 – 1738
Helixi181 – 1899
Beta strandi200 – 20910
Beta strandi215 – 2239
Turni224 – 2274
Beta strandi228 – 2314

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4A8JX-ray2.10B/E1-270[»]
4EJSX-ray2.61B1-238[»]
ProteinModelPortaliP38874.
SMRiP38874. Positions 7-234.

Family & Domainsi

Sequence similaritiesi

Belongs to the ELP5 family.

Phylogenomic databases

eggNOGiNOG72129.
HOGENOMiHOG000248152.
KOiK11376.
OMAiTNIIYLS.
OrthoDBiEOG7XWQ03.

Family and domain databases

InterProiIPR019519. Elp5.
[Graphical view]
PfamiPF10483. Elong_Iki1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P38874-1 [UniParc]FASTAAdd to Basket

« Hide

MASSSHNPVI LLKRILSLTE SSPFILCLDS IAQTSYKLIQ EFVHQSKSKG    50
NEYPIVYISF ETVNKPSYCT QFIDATQMDF VHLVKQIISY LPAATATQAK 100
KHMVIIDSLN YISTEYITRF LSEIASPHCT MVATYHKDIK DENRTVIPDW 150
NNNYPDKLTL LQFMATTIVD IDVVLTGTLD TEEVSELLNE FRIPRGLNND 200
IFQLRLVNKR KSGRSLEYDF IVNSNTHEYE LLSTTKQEEE SSSNGLETPE 250
MLQGLTTFNL GTSNKQKLAK DQVALPFLEA QSFGQGGAIV YEYEKDDDYD 300
EEDPYEDPF 309
Length:309
Mass (Da):35,220
Last modified:February 1, 1995 - v1
Checksum:iDFD93D7BA583E2F5
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
D83267 Genomic DNA. Translation: BAA20087.1.
U00030 Genomic DNA. Translation: AAB68362.1.
BK006934 Genomic DNA. Translation: DAA06879.1.
PIRiS46684.
RefSeqiNP_012057.3. NM_001179318.3.

Genome annotation databases

EnsemblFungiiYHR187W; YHR187W; YHR187W.
GeneIDi856594.
KEGGisce:YHR187W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
D83267 Genomic DNA. Translation: BAA20087.1 .
U00030 Genomic DNA. Translation: AAB68362.1 .
BK006934 Genomic DNA. Translation: DAA06879.1 .
PIRi S46684.
RefSeqi NP_012057.3. NM_001179318.3.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
4A8J X-ray 2.10 B/E 1-270 [» ]
4EJS X-ray 2.61 B 1-238 [» ]
ProteinModelPortali P38874.
SMRi P38874. Positions 7-234.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 36621. 118 interactions.
DIPi DIP-1967N.
IntActi P38874. 9 interactions.
MINTi MINT-385467.
STRINGi 4932.YHR187W.

Proteomic databases

MaxQBi P38874.
PaxDbi P38874.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YHR187W ; YHR187W ; YHR187W .
GeneIDi 856594.
KEGGi sce:YHR187W.

Organism-specific databases

CYGDi YHR187w.
SGDi S000001230. IKI1.

Phylogenomic databases

eggNOGi NOG72129.
HOGENOMi HOG000248152.
KOi K11376.
OMAi TNIIYLS.
OrthoDBi EOG7XWQ03.

Enzyme and pathway databases

BioCyci YEAST:G3O-31217-MONOMER.

Miscellaneous databases

NextBioi 982478.
PROi P38874.

Gene expression databases

Genevestigatori P38874.

Family and domain databases

InterProi IPR019519. Elp5.
[Graphical view ]
Pfami PF10483. Elong_Iki1. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Characterization of IKI1 and IKI3 genes conferring pGKL killer sensitivity on Saccharomyces cerevisiae."
    Yajima H., Tokunaga M., Nakayama-Murayama A., Hishinuma F.
    Biosci. Biotechnol. Biochem. 61:704-709(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  4. "Elongator, a multisubunit component of a novel RNA polymerase II holoenzyme for transcriptional elongation."
    Otero G., Fellows J., Li Y., de Bizemont T., Dirac A.M., Gustafsson C.M., Erdjument-Bromage H., Tempst P., Svejstrup J.Q.
    Mol. Cell 3:109-118(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION OF THE RNA POLYMERASE II ELONGATOR COMPLEX IN TRANSCRIPTIONAL REGULATION, SUBCELLULAR LOCATION.
  5. "Saccharomyces cerevisiae Elongator mutations confer resistance to the Kluyveromyces lactis zymocin."
    Frohloff F., Fichtner L., Jablonowski D., Breunig K.D., Schaffrath R.
    EMBO J. 20:1993-2003(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION OF THE RNA POLYMERASE II ELONGATOR COMPLEX IN ZYMOCIN SENSITIVITY.
  6. "RNA polymerase II elongator holoenzyme is composed of two discrete subcomplexes."
    Winkler G.S., Petrakis T.G., Ethelberg S., Tokunaga M., Erdjument-Bromage H., Tempst P., Svejstrup J.Q.
    J. Biol. Chem. 276:32743-32749(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION IN THE RNA POLYMERASE II ELONGATOR COMPLEX.
  7. "A multiprotein complex that interacts with RNA polymerase II elongator."
    Li Y., Takagi Y., Jiang Y., Tokunaga M., Erdjument-Bromage H., Tempst P., Kornberg R.D.
    J. Biol. Chem. 276:29628-29631(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION IN THE RNA POLYMERASE II ELONGATOR COMPLEX, IDENTIFICATION BY MASS SPECTROMETRY.
  8. "Characterization of a six-subunit holo-elongator complex required for the regulated expression of a group of genes in Saccharomyces cerevisiae."
    Krogan N.J., Greenblatt J.F.
    Mol. Cell. Biol. 21:8203-8212(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION IN THE RNA POLYMERASE II ELONGATOR COMPLEX, FUNCTION OF THE RNA POLYMERASE II ELONGATOR COMPLEX IN TRANSCRIPTIONAL REGULATION.
  9. "Elongator is a histone H3 and H4 acetyltransferase important for normal histone acetylation levels in vivo."
    Winkler G.S., Kristjuhan A., Erdjument-Bromage H., Tempst P., Svejstrup J.Q.
    Proc. Natl. Acad. Sci. U.S.A. 99:3517-3522(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION OF THE RNA POLYMERASE II ELONGATOR COMPLEX IN HISTONE ACETYLATION.
  10. "Structural and functional analysis of the killer element pPin1-3 from Pichia inositovora."
    Klassen R., Meinhardt F.
    Mol. Genet. Genomics 270:190-199(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION OF THE RNA POLYMERASE II ELONGATOR COMPLEX IN PICHIA INOSITOVORA TOXIN SENSITIVITY.
  11. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
  12. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  13. "Elp1p, the yeast homolog of the FD disease syndrome protein, negatively regulates exocytosis independently of transcriptional elongation."
    Rahl P.B., Chen C.Z., Collins R.N.
    Mol. Cell 17:841-853(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION.
  14. "An early step in wobble uridine tRNA modification requires the Elongator complex."
    Huang B., Johansson M.J.O., Bystroem A.S.
    RNA 11:424-436(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION IN TRNA MODIFICATION.

Entry informationi

Entry nameiELP5_YEAST
AccessioniPrimary (citable) accession number: P38874
Secondary accession number(s): D3DLD5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: February 1, 1995
Last modified: September 3, 2014
This is version 115 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 3870 molecules/cell in log phase SD medium.

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome VIII
    Yeast (Saccharomyces cerevisiae) chromosome VIII: entries and gene names

External Data

Dasty 3

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