P38863 (SPC97_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Spindle pole body component SPC97

Gene names

Name:	SPC97
Ordered Locus Names:	YHR172W

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › Saccharomyces cerevisiae

Protein attributes

Sequence length	823 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Involved in microtubule organization by the microtubule organizing center, the spindle pole body (SPB). Probably part of the microtubule attachment site at the SPB.
Subunit structure	Interacts with TUB4, SPC72 and SPC98. Ref.4
Subcellular location	Nucleus. Cytoplasm › cytoskeleton › microtubule organizing center › spindle pole body.
Miscellaneous	Present with 2230 molecules/cell in log phase SD medium.
Sequence similarities	Belongs to the TUBGCP family.

Ontologies

Keywords
Cellular component	Cytoplasm Cytoskeleton Microtubule Nucleus
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	microtubule nucleation Traceable author statement PubMed 9153752. Source: SGD
Cellular_component	gamma-tubulin small complex, spindle pole body Inferred from physical interaction PubMed 9384578. Source: SGD inner plaque of spindle pole body Inferred from direct assay PubMed 9215630. Source: SGD microtubule Inferred from electronic annotation. Source: UniProtKB-KW nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell outer plaque of spindle pole body Inferred from direct assay PubMed 9215630. Source: SGD
Molecular_function	protein binding Inferred from physical interaction PubMed 10688190PubMed 11805826PubMed 16429126. Source: IntAct structural constituent of cytoskeleton Inferred from physical interaction PubMed 9215630. Source: SGD
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
SPC98	P53540	3	EBI-17786,EBI-17794
TUB4	P53378	4	EBI-17786,EBI-19013

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 823	823	Spindle pole body component SPC97		PRO_0000078117

Sequences

	Sequence		Length	Mass (Da)
	P38863 [UniParc]. Last modified February 1, 1995. Version 1. Checksum: A3F7018957D96E8D	FASTA	823	96,825
	10 20 30 40 50 60 MEIKEVDDRA ELLRYTNNIP LLGKLVNHQP LWSTNPKLKS FSLEKISAPD QRRVQEALVV 70 80 90 100 110 120 KDLLNVLIGL EGTYIRYFND YEPSDPETPI EFKIAKKMDP SFKTFSRRIV RYGKQYMILT 130 140 150 160 170 180 RAYEKWSDTS FGMVLQRFAY EIRRFLEDVY LKTLVERLER DFNKVPNFSI RELEQIINET 190 200 210 220 230 240 EVNKQMELLY NIYEEIFREI EERRTNQSSQ EDFNNFMDSM KNESSLHLRL MVAFDTTVYP 250 260 270 280 290 300 VPKGGAILKI FQQKILENLG DRSSVMFLKK LLNNISQDYC TMLYEWLTQG ILNDPYQEFM 310 320 330 340 350 360 TYDDLEGKTD NIFDTRDRAW DTQYFIRKDV LLRDCDSEED KNLLFKMLRT GILLKVVRAS 370 380 390 400 410 420 LQIPTIPSNS SDITIQEIND FADLMEGSNL ELYVDKCYSR ANEIFLKLFF QGYDLINVLK 430 440 450 460 470 480 HLQQIFLGYQ SGHNVLKFLT KNMGELTKHY RNDNNANYDK LLQNFELERQ SENPNNLMRQ 490 500 510 520 530 540 LLMIQFDTET LPQVLSHYLQ IYPEVPENNS ANDDSDPLMH ANNFKNMNAI LFDELSKERT 550 560 570 580 590 600 GAYHGSNLEL YTPKSAIYHL KFDINIPYPL NIIISRTCMI KYQIILRYQL VLQYHSRLLD 610 620 630 640 650 660 ETWMDLNKTP SWKYRGYSHT VKRRIVRATR VLHAKMNHFI KTIMEYFNQN VIDKEVYSLE 670 680 690 700 710 720 KCYRNPTLAV AIQNELEGGL TNIMTNRCLS DLIPLQLQIF DIVYKFCKFI KSMRAKLCQL 730 740 750 760 770 780 DPVLYEKHKS GMMKTLNEGY RTNNGGQEDV GYQEDAALEL IQKLIEYISN ASSIFRKCLI 790 800 810 820 NFTQELSTEK FDFYDSSSVD AAGIERVLYS IVPPRSASAS SQR

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Complete nucleotide sequence of Saccharomyces cerevisiae chromosome VIII." Johnston M., Andrews S., Brinkman R., Cooper J., Ding H., Dover J., Du Z., Favello A., Fulton L., Gattung S., Geisel C., Kirsten J., Kucaba T., Hillier L.W., Jier M., Johnston L., Langston Y., Latreille P. , Louis E.J., Macri C., Mardis E., Menezes S., Mouser L., Nhan M., Rifkin L., Riles L., St Peter H., Trevaskis E., Vaughan K., Vignati D., Wilcox L., Wohldman P., Waterston R., Wilson R., Vaudin M. Science 265:2077-2082(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[2]	"The reference genome sequence of Saccharomyces cerevisiae: Then and now." Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R., Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S., Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M. G3 (Bethesda) 4:389-398(2014) [PubMed] [Europe PMC] [Abstract] Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[3]	"The spindle pole body component Spc97p interacts with the gamma-tubulin of Saccharomyces cerevisiae and functions in microtubule organization and spindle pole body duplication." Knop M., Pereira G., Geissler S., Grein K., Schiebel E. EMBO J. 16:1550-1564(1997) [PubMed] [Europe PMC] [Abstract] Cited for: CHARACTERIZATION.
[4]	"Receptors determine the cellular localization of a gamma-tubulin complex and thereby the site of microtubule formation." Knop M., Schiebel E. EMBO J. 17:3952-3967(1998) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH SPC72.
[5]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[6]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[7]	"N-terminal acetylome analyses and functional insights of the N-terminal acetyltransferase NatB." Van Damme P., Lasa M., Polevoda B., Gazquez C., Elosegui-Artola A., Kim D.S., De Juan-Pardo E., Demeyer K., Hole K., Larrea E., Timmerman E., Prieto J., Arnesen T., Sherman F., Gevaert K., Aldabe R. Proc. Natl. Acad. Sci. U.S.A. 109:12449-12454(2012) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U00027 Genomic DNA. Translation: AAB68017.1. BK006934 Genomic DNA. Translation: DAA06865.1.
PIR	S48911.
RefSeq	NP_012042.1. NM_001179303.1.
3D structure databases
ProteinModelPortal	P38863.
ModBase	Search...
MobiDB	Search...
Protein-protein interaction databases
BioGrid	36606. 17 interactions.
DIP	DIP-826N.
IntAct	P38863. 14 interactions.
MINT	MINT-477633.
STRING	4932.YHR172W.
Proteomic databases
MaxQB	P38863.
PaxDb	P38863.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YHR172W; YHR172W; YHR172W.
GeneID	856577.
KEGG	sce:YHR172W.
Organism-specific databases
CYGD	YHR172w.
SGD	S000001215. SPC97.
Phylogenomic databases
eggNOG	KOG2001.
HOGENOM	HOG000144398.
KO	K16569.
OMA	RANKLLM.
OrthoDB	EOG77Q554.
Enzyme and pathway databases
BioCyc	YEAST:G3O-31206-MONOMER.
Gene expression databases
Genevestigator	P38863.
Family and domain databases
InterPro	IPR007259. TUBGCP. [Graphical view]
PANTHER	PTHR19302. PTHR19302. 1 hit.
Pfam	PF04130. Spc97_Spc98. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	982435.
PRO	P38863.

Entry information

Entry name

SPC97_YEAST

Accession

Primary (citable) accession number: P38863
Secondary accession number(s): D3DLC1

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1995
Last sequence update:	February 1, 1995
Last modified:	July 9, 2014
This is version 103 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program