P38855 (PEX18_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Peroxisomal membrane protein PEX18

Alternative name(s):
Peroxin-18

Gene names

Name:	PEX18
Ordered Locus Names:	YHR160C

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › Saccharomyces cerevisiae

Protein attributes

Sequence length	283 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Involved in peroxisome biogenesis and the import of peroxisomal matrix proteins that contain the peroxisomal targeting sequence PTS2. Required for peroxisomal targeting of PEX7 and growth on oleate. Ref.3 Ref.5 Ref.6
Subunit structure	Interacts with PEX7 and PEX13. The interaction with PEX7 stabilizes PEX18. Ref.3 Ref.4 Ref.5
Subcellular location	Cytoplasm. Peroxisome membrane; Peripheral membrane protein; Cytoplasmic side Ref.3.
Post-translational modification	Ubiquitinated in a UBC4/UBC5 dependent manner. Ref.4

Ontologies

Keywords
Cellular component	Cytoplasm Membrane Peroxisome
PTM	Ubl conjugation
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	protein import into peroxisome matrix Inferred from mutant phenotype Ref.3. Source: SGD
Cellular_component	cytosol Inferred from direct assay Ref.3. Source: SGD peroxisomal membrane Inferred from electronic annotation. Source: UniProtKB-SubCell peroxisome Inferred from direct assay Ref.3. Source: SGD
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
PEX7	P39108	5	EBI-24803,EBI-13183

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 283	283	Peroxisomal membrane protein PEX18		PRO_0000202929

Sequences

	Sequence		Length	Mass (Da)
	P38855 [UniParc]. Last modified February 1, 1995. Version 1. Checksum: 574C644FD7ED7A71	FASTA	283	32,037
	10 20 30 40 50 60 MNSNRCQTNE VNKFISSTEK GPFTGRDNTL SFNKIGSRLN SPPILKDKIE LKFLQHSEDL 70 80 90 100 110 120 NQSRSYVNIR PRTLEDQSYK FEAPNLNDNE TSWAKDFRYN FPKNVEPPIE NQIANLNINN 130 140 150 160 170 180 GLRTSQTDFP LGFYSQKNFN IASFPVVDHQ IFKTTGLEHP INSHIDSLIN AEFSELEASS 190 200 210 220 230 240 LEEDVHTEEE NSGTSLEDEE TAMKGLASDI IEFCDNNSAN KDVKERLNSS KFMGLMGSIS 250 260 270 280 DGSIVLKKDN GTERNLQKHV GFCFQNSGNW AGLEFHDVED RIA

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Complete nucleotide sequence of Saccharomyces cerevisiae chromosome VIII." Johnston M., Andrews S., Brinkman R., Cooper J., Ding H., Dover J., Du Z., Favello A., Fulton L., Gattung S., Geisel C., Kirsten J., Kucaba T., Hillier L.W., Jier M., Johnston L., Langston Y., Latreille P. , Louis E.J., Macri C., Mardis E., Menezes S., Mouser L., Nhan M., Rifkin L., Riles L., St Peter H., Trevaskis E., Vaughan K., Vignati D., Wilcox L., Wohldman P., Waterston R., Wilson R., Vaudin M. Science 265:2077-2082(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[2]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[3]	"Pex18p and Pex21p, a novel pair of related peroxins essential for peroxisomal targeting by the PTS2 pathway." Purdue P.E., Yang X., Lazarow P.B. J. Cell Biol. 143:1859-1869(1998) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION, INTERACTION WITH PEX7.
[4]	"Pex18p is constitutively degraded during peroxisome biogenesis." Purdue P.E., Lazarow P.B. J. Biol. Chem. 276:47684-47689(2001) [PubMed] [Europe PMC] [Abstract] Cited for: UBIQUITINATION, INTERACTION WITH PEX7, STABILITY.
[5]	"Interactions of Pex7p and Pex18p/Pex21p with the peroxisomal docking machinery: implications for the first steps in PTS2 protein import." Stein K., Schell-Steven A., Erdmann R., Rottensteiner H. Mol. Cell. Biol. 22:6056-6069(2002) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, INTERACTION WITH PEX7 AND PEX13.
[6]	"Functional similarity between the peroxisomal PTS2 receptor binding protein Pex18p and the N-terminal half of the PTS1 receptor Pex5p." Schaefer A., Kerssen D., Veenhuis M., Kunau W.-H., Schliebs W. Mol. Cell. Biol. 24:8895-8906(2004) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U10397 Genomic DNA. Translation: AAB68992.1. BK006934 Genomic DNA. Translation: DAA06853.1.
PIR	S46770.
RefSeq	NP_012030.1. NM_001179291.1.
3D structure databases
ProteinModelPortal	P38855.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-1502N.
IntAct	P38855. 5 interactions.
MINT	MINT-409610.
STRING	4932.YHR160C.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YHR160C; YHR160C; YHR160C.
GeneID	856565.
KEGG	sce:YHR160C.
Organism-specific databases
CYGD	YHR160c.
SGD	S000001203. PEX18.
Gene expression databases
Genevestigator	P38855.
GermOnline	YHR160C. Saccharomyces cerevisiae.
Family and domain databases
ProtoNet	Search...
Other
NextBio	982399.

Entry information

Entry name

PEX18_YEAST

Accession

Primary (citable) accession number: P38855
Secondary accession number(s): D3DLA9

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1995
Last sequence update:	February 1, 1995
Last modified:	April 3, 2013
This is version 73 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program