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P38850

- RT107_YEAST

UniProt

P38850 - RT107_YEAST

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Protein
Regulator of Ty1 transposition protein 107
Gene
RTT107, ESC4, YHR154W
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at protein leveli

Functioni

Required for resumption of chromosome replication after DNA damage, specifically in S phase. Is recruited to chromatin in the presence of RTT109 and RTT101 in response to stalled replication forks and acts as a scaffold during DNA repair.2 Publications

GO - Biological processi

  1. double-strand break repair Source: SGD
  2. negative regulation of transposition, RNA-mediated Source: SGD
Complete GO annotation...

Keywords - Biological processi

DNA damage, DNA repair

Enzyme and pathway databases

BioCyciYEAST:G3O-31189-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Regulator of Ty1 transposition protein 107
Alternative name(s):
Establishes silent chromatin protein 4
Gene namesi
Name:RTT107
Synonyms:ESC4
Ordered Locus Names:YHR154W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome VIII

Organism-specific databases

CYGDiYHR154w.
SGDiS000001197. RTT107.

Subcellular locationi

Nucleus
Note: Recruited to chromatin in response to replication fork stalling.2 Publications

GO - Cellular componenti

  1. Cul8-RING ubiquitin ligase complex Source: SGD
  2. nucleus Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 10701070Regulator of Ty1 transposition protein 107
PRO_0000097502Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei304 – 3041Phosphoserine1 Publication
Modified residuei532 – 5321Phosphothreonine3 Publications
Modified residuei591 – 5911Phosphoserine1 Publication
Modified residuei593 – 5931Phosphoserine1 Publication
Modified residuei720 – 7201Phosphoserine1 Publication
Modified residuei800 – 8001Phosphoserine1 Publication
Modified residuei806 – 8061Phosphoserine2 Publications

Post-translational modificationi

Phosphorylated by MEC1.1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP38850.
PaxDbiP38850.
PeptideAtlasiP38850.

Expressioni

Gene expression databases

GenevestigatoriP38850.

Interactioni

Subunit structurei

Forms a complex with the cullin-RING ligase (CRL) RTT101(MMS1-MMS22). Interacts with MMS22 and RTT101. Interacts with histone H2A; requires H2A to be phosphorylated (gamma-H2A). Interacts with RAD55.3 Publications

Protein-protein interaction databases

BioGridi36588. 353 interactions.
DIPiDIP-6297N.
IntActiP38850. 13 interactions.
MINTiMINT-702893.
STRINGi4932.YHR154W.

Structurei

Secondary structure

Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi822 – 8276
Beta strandi837 – 8448
Helixi851 – 8599
Beta strandi862 – 8643
Helixi870 – 8723
Beta strandi876 – 8783
Helixi886 – 8916
Beta strandi899 – 9013
Helixi904 – 91310
Helixi927 – 9304
Helixi937 – 9426
Beta strandi945 – 9473
Helixi949 – 9524
Beta strandi957 – 9615
Helixi968 – 97710
Beta strandi982 – 9865
Turni988 – 9903
Helixi993 – 9953
Beta strandi1011 – 10155
Helixi1019 – 103214
Beta strandi1038 – 10414
Helixi1043 – 10519
Beta strandi1063 – 10675

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3T7IX-ray2.30A/B820-1070[»]
3T7JX-ray2.04A/B820-1070[»]
3T7KX-ray2.03A/B820-1070[»]
ProteinModelPortaliP38850.
SMRiP38850. Positions 821-1068.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini2 – 103102BRCT 1
Add
BLAST
Domaini117 – 21397BRCT 2
Add
BLAST
Domaini260 – 35293BRCT 3
Add
BLAST
Domaini369 – 45385BRCT 4
Add
BLAST
Domaini829 – 91082BRCT 5
Add
BLAST
Domaini934 – 1049116BRCT 6
Add
BLAST

Sequence similaritiesi

Contains 6 BRCT domains.

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiNOG248673.
OMAiRINTIFA.
OrthoDBiEOG769ZTW.

Family and domain databases

Gene3Di3.40.50.10190. 2 hits.
InterProiIPR001357. BRCT_dom.
[Graphical view]
PfamiPF00533. BRCT. 2 hits.
[Graphical view]
SMARTiSM00292. BRCT. 4 hits.
[Graphical view]
SUPFAMiSSF52113. SSF52113. 2 hits.
PROSITEiPS50172. BRCT. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P38850-1 [UniParc]FASTAAdd to Basket

« Hide

MSTSLLFEQL NFLILVAAEA ELPIAHSTRK LLMDNSCNNC QIYELYNENL     50
KDVKTDKDWF MNKFGPQTVH FVISNTINFP FYKIVYFDLL IPVVSHTWVQ 100
DSVKTKRHLR TNMYSPNPFH LLRDCQVYIS KSSFNKCEYI LYSDLLHLLG 150
GTLVNYISNR TTHVIVQSPQ DPIIATVSKL TFGSFSSSST NKHTEKPLRE 200
WKFVYPIWIL YHFKMAKPLK GELATLCELD MQDTSEEQLF AKWEEVIGDK 250
QTSSSQLTLH PNKTLFKNHH FAISPDLNFF TPLYWFLKGF IEDLDGKVTP 300
LSFSDDLKSV YQAFPDIDCY IGHSANSPIL EKTKSIKPEI HVGNVSWLFY 350
MFALQKFTPV SQCKLIHQPF HAKLFTSKEL TVAYTNYFGS QRFYIQRLVE 400
ILGGLSTPEL TRKNTHLITK STIGKKFKVA KKWSLDPQNA IIVTNHMWLE 450
QCYMNNSKLN PKDSRFQNFK LDDNMGWNIG QIGMDHSSLP TPKNLSMVTY 500
DTQSISEKPP PTNDQMDQIN DNTNVLSKKD GTPISSFENS IDEKIDKLQK 550
ISGEVAVTHS GDLERSFVSR PSRASFPVVD SKKSNLQKKD SNSDISMETE 600
VFCEGHEKRE EKEFTKPITE YDAPKKQEIR EQSRKKNDID YKKEEEETEL 650
QVQLGQRTKR EIKTSKKNEK EKETNECHIE VDQMTNEKQG EESTGKLIST 700
EDVTSKKDTD KFSHLFEGLS DNDDHINDEK PAVNSKYTTP KTSQNITSGV 750
DTPTTAQTQV FMPSSGNSRL AKTQAAKRLH TDIESLNEFQ KNFKRKRIDS 800
EEISLSQDVE RSNNNKELAT KAEKILARFN ELPNYDLKAV CTGCFHDGFN 850
EVDIEILNQL GIKIFDNIKE TDKLNCIFAP KILRTEKFLK SLSFEPLKFA 900
LKPEFIIDLL KQIHSKKDKL SQININLFDY EINGINESII SKTKLPTKVF 950
ERANIRCINL VNDIPGGVDT IGSVLKAHGI EKINVLRSKK CTFEDIIPND 1000
VSKQENGGIF KYVLIVTKAS QVKKFTKLIN DRDKNETILI VEWNWCVESI 1050
FHLNVDFTSK KNVLYQKKNN 1070
Length:1,070
Mass (Da):123,017
Last modified:February 1, 1995 - v1
Checksum:i767931285BB52580
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U10397 Genomic DNA. Translation: AAB68978.1.
BK006934 Genomic DNA. Translation: DAA06847.1.
PIRiS46755.
RefSeqiNP_012024.1. NM_001179285.1.

Genome annotation databases

EnsemblFungiiYHR154W; YHR154W; YHR154W.
GeneIDi856559.
KEGGisce:YHR154W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U10397 Genomic DNA. Translation: AAB68978.1 .
BK006934 Genomic DNA. Translation: DAA06847.1 .
PIRi S46755.
RefSeqi NP_012024.1. NM_001179285.1.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
3T7I X-ray 2.30 A/B 820-1070 [» ]
3T7J X-ray 2.04 A/B 820-1070 [» ]
3T7K X-ray 2.03 A/B 820-1070 [» ]
ProteinModelPortali P38850.
SMRi P38850. Positions 821-1068.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 36588. 353 interactions.
DIPi DIP-6297N.
IntActi P38850. 13 interactions.
MINTi MINT-702893.
STRINGi 4932.YHR154W.

Proteomic databases

MaxQBi P38850.
PaxDbi P38850.
PeptideAtlasi P38850.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YHR154W ; YHR154W ; YHR154W .
GeneIDi 856559.
KEGGi sce:YHR154W.

Organism-specific databases

CYGDi YHR154w.
SGDi S000001197. RTT107.

Phylogenomic databases

eggNOGi NOG248673.
OMAi RINTIFA.
OrthoDBi EOG769ZTW.

Enzyme and pathway databases

BioCyci YEAST:G3O-31189-MONOMER.

Miscellaneous databases

NextBioi 982381.

Gene expression databases

Genevestigatori P38850.

Family and domain databases

Gene3Di 3.40.50.10190. 2 hits.
InterProi IPR001357. BRCT_dom.
[Graphical view ]
Pfami PF00533. BRCT. 2 hits.
[Graphical view ]
SMARTi SM00292. BRCT. 4 hits.
[Graphical view ]
SUPFAMi SSF52113. SSF52113. 2 hits.
PROSITEi PS50172. BRCT. 2 hits.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  2. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  3. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
  4. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  5. "Esc4p, a new target of Mec1p (ATR), promotes resumption of DNA synthesis after DNA damage."
    Rouse J.
    EMBO J. 23:1188-1197(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, PHOSPHORYLATION BY MEC1.
  6. "Esc4/Rtt107 and the control of recombination during replication."
    Chin J.K., Bashkirov V.I., Heyer W.-D., Romesberg F.E.
    DNA Repair 5:618-628(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH RAD55 AND MMS22, SUBCELLULAR LOCATION.
  7. "Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
    Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
    J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-532, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Strain: ADR376.
  8. "Regulation of rtt107 recruitment to stalled DNA replication forks by the cullin rtt101 and the rtt109 acetyltransferase."
    Roberts T.M., Zaidi I.W., Vaisica J.A., Peter M., Brown G.W.
    Mol. Biol. Cell 19:171-180(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH RTT101.
  9. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
    Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
    Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-304; THR-532; SER-591; SER-593; SER-800 AND SER-806, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  10. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
    Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
    Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-532; SER-720 AND SER-806, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  11. "Cul8/Rtt101 forms a variety of protein complexes that regulate DNA damage response and transcriptional silencing."
    Mimura S., Yamaguchi T., Ishii S., Noro E., Katsura T., Obuse C., Kamura T.
    J. Biol. Chem. 285:9858-9867(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH MMS22, IDENTIFICATION IN A COMPLEX WITH RTT101 AND MMS1.
  12. "Structure of C-terminal tandem BRCT repeats of Rtt107 protein reveals critical role in interaction with phosphorylated histone H2A during DNA damage repair."
    Li X., Liu K., Li F., Wang J., Huang H., Wu J., Shi Y.
    J. Biol. Chem. 287:9137-9146(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.03 ANGSTROMS) OF 820-1070 IN COMPLEX WITH HTA1.

Entry informationi

Entry nameiRT107_YEAST
AccessioniPrimary (citable) accession number: P38850
Secondary accession number(s): D3DLA3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: February 1, 1995
Last modified: September 3, 2014
This is version 114 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 1380 molecules/cell in log phase SD medium.

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome VIII
    Yeast (Saccharomyces cerevisiae) chromosome VIII: entries and gene names

External Data

Dasty 3

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