ID   PEX28_YEAST             Reviewed;         579 AA.
AC   P38848; D3DL99;
DT   01-FEB-1995, integrated into UniProtKB/Swiss-Prot.
DT   01-FEB-1995, sequence version 1.
DT   27-MAR-2024, entry version 148.
DE   RecName: Full=Peroxisomal membrane protein PEX28;
DE   AltName: Full=Peroxin-28;
GN   Name=PEX28; OrderedLocusNames=YHR150W;
OS   Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC   Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX   NCBI_TaxID=559292;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 204508 / S288c;
RX   PubMed=8091229; DOI=10.1126/science.8091229;
RA   Johnston M., Andrews S., Brinkman R., Cooper J., Ding H., Dover J., Du Z.,
RA   Favello A., Fulton L., Gattung S., Geisel C., Kirsten J., Kucaba T.,
RA   Hillier L.W., Jier M., Johnston L., Langston Y., Latreille P., Louis E.J.,
RA   Macri C., Mardis E., Menezes S., Mouser L., Nhan M., Rifkin L., Riles L.,
RA   St Peter H., Trevaskis E., Vaughan K., Vignati D., Wilcox L., Wohldman P.,
RA   Waterston R., Wilson R., Vaudin M.;
RT   "Complete nucleotide sequence of Saccharomyces cerevisiae chromosome
RT   VIII.";
RL   Science 265:2077-2082(1994).
RN   [2]
RP   GENOME REANNOTATION.
RC   STRAIN=ATCC 204508 / S288c;
RX   PubMed=24374639; DOI=10.1534/g3.113.008995;
RA   Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA   Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA   Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT   "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL   G3 (Bethesda) 4:389-398(2014).
RN   [3]
RP   FUNCTION, AND SUBCELLULAR LOCATION.
RX   PubMed=12707309; DOI=10.1083/jcb.200210130;
RA   Vizeacoumar F.J., Torres-Guzman J.C., Tam Y.Y.C., Aitchison J.D.,
RA   Rachubinski R.A.;
RT   "YHR150w and YDR479c encode peroxisomal integral membrane proteins involved
RT   in the regulation of peroxisome number, size, and distribution in
RT   Saccharomyces cerevisiae.";
RL   J. Cell Biol. 161:321-332(2003).
RN   [4]
RP   TOPOLOGY [LARGE SCALE ANALYSIS].
RC   STRAIN=ATCC 208353 / W303-1A;
RX   PubMed=16847258; DOI=10.1073/pnas.0604075103;
RA   Kim H., Melen K., Oesterberg M., von Heijne G.;
RT   "A global topology map of the Saccharomyces cerevisiae membrane proteome.";
RL   Proc. Natl. Acad. Sci. U.S.A. 103:11142-11147(2006).
CC   -!- FUNCTION: Involved in the regulation of peroxisome number, size and
CC       distribution. {ECO:0000269|PubMed:12707309}.
CC   -!- SUBCELLULAR LOCATION: Peroxisome membrane
CC       {ECO:0000269|PubMed:12707309}; Multi-pass membrane protein
CC       {ECO:0000269|PubMed:12707309}.
CC   -!- SIMILARITY: Belongs to the peroxin-2832 family. Peroxin-28 subfamily.
CC       {ECO:0000305}.
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DR   EMBL; U10397; AAB68980.1; -; Genomic_DNA.
DR   EMBL; BK006934; DAA06843.1; -; Genomic_DNA.
DR   PIR; S46757; S46757.
DR   RefSeq; NP_012020.1; NM_001179281.1.
DR   AlphaFoldDB; P38848; -.
DR   BioGRID; 36584; 63.
DR   STRING; 4932.YHR150W; -.
DR   TCDB; 3.A.20.1.5; the peroxisomal protein importer (ppi) family.
DR   GlyCosmos; P38848; 1 site, No reported glycans.
DR   GlyGen; P38848; 1 site.
DR   iPTMnet; P38848; -.
DR   MaxQB; P38848; -.
DR   PaxDb; 4932-YHR150W; -.
DR   PeptideAtlas; P38848; -.
DR   EnsemblFungi; YHR150W_mRNA; YHR150W; YHR150W.
DR   GeneID; 856555; -.
DR   KEGG; sce:YHR150W; -.
DR   AGR; SGD:S000001193; -.
DR   SGD; S000001193; PEX28.
DR   VEuPathDB; FungiDB:YHR150W; -.
DR   eggNOG; ENOG502QUW8; Eukaryota.
DR   HOGENOM; CLU_034954_0_0_1; -.
DR   InParanoid; P38848; -.
DR   OMA; WRLIFIQ; -.
DR   OrthoDB; 1367449at2759; -.
DR   BioCyc; YEAST:G3O-31185-MONOMER; -.
DR   BioGRID-ORCS; 856555; 0 hits in 10 CRISPR screens.
DR   PRO; PR:P38848; -.
DR   Proteomes; UP000002311; Chromosome VIII.
DR   RNAct; P38848; Protein.
DR   GO; GO:0005829; C:cytosol; HDA:SGD.
DR   GO; GO:0005778; C:peroxisomal membrane; IDA:SGD.
DR   GO; GO:0007031; P:peroxisome organization; IDA:SGD.
DR   InterPro; IPR010482; Peroxin.
DR   PANTHER; PTHR28304:SF1; PEROXISOMAL MEMBRANE PROTEIN PEX28; 1.
DR   PANTHER; PTHR28304; PEROXISOMAL MEMBRANE PROTEIN PEX29; 1.
DR   Pfam; PF06398; Pex24p; 1.
PE   1: Evidence at protein level;
KW   Glycoprotein; Membrane; Peroxisome; Peroxisome biogenesis;
KW   Reference proteome; Transmembrane; Transmembrane helix.
FT   CHAIN           1..579
FT                   /note="Peroxisomal membrane protein PEX28"
FT                   /id="PRO_0000202925"
FT   TOPO_DOM        1..243
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        244..264
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        265..392
FT                   /note="Peroxisomal"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        393..413
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        414..579
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   REGION          40..70
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        44..70
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   CARBOHYD        361
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   579 AA;  66148 MW;  2A6A73999C54C19E CRC64;
     MSETSSSRRS ASKDAVKSYF AGKYNKVLDS ILEAEAAISK SPTVAEDLSG SSSSGNSEMS
     HPSLTASSAT SQGISKKELL QQIAGSLFST SIERLKTAHS SEVSSTPEYS VNDSYGEQEC
     RECDGVFKCS AHFEGAPEYY DDETESGPAL EPMTSNSEKD PFIDVFLDKL ISRLVPEKLP
     EREHFSSKTT IEHDLDTGRV PVFSATTLGS NFKKLSKKMG SIFELQDSIV RLLTWRNPTG
     TVTSLILFTL ICFNPMYLVI LPIFRFVYGI VVPGYVRKHP LQRSIYPLKR NHGSSLLYDV
     CYEGKNEYSY GQQFFSKSFM DTLESRNQEI DEISELDKRT ENTGELKQGM KVLINLRDMQ
     NMTSGTLHVI EAINSFLRKS SSFQNEECST KRFFTGFLLI VFLKILSPFV NWSYVCSIFA
     WCLLIYMHPR AHPKIISFFK TGTMGKEYKN LKKREHQALN MIFDEQPETK FIEIFEIYKK
     ALLPNDWKFF RYSNRIFDPQ DPYRRAQQFP PGVDSLADVI PPTGWSFDPN FEWKIDNDVD
     RWVVERGLNL PITGEFLFDP MFKRRRLIHR VIKNATPVA
//