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P38845

- CRP1_YEAST

UniProt

P38845 - CRP1_YEAST

Protein

Cruciform DNA-recognizing protein 1

Gene

CRP1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 97 (01 Oct 2014)
      Sequence version 1 (01 Feb 1995)
      Previous versions | rss
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    Functioni

    Cruciform DNA-binding protein which exerts an enhancing effect on the cleavage of cruciform DNA (X-DNA) by endonuclease VII from bacteriophage T4.1 Publication

    GO - Molecular functioni

    1. DNA binding Source: SGD

    Keywords - Ligandi

    DNA-binding

    Enzyme and pathway databases

    BioCyciYEAST:G3O-31181-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Cruciform DNA-recognizing protein 1
    Cleaved into the following 2 chains:
    Gene namesi
    Name:CRP1
    Ordered Locus Names:YHR146W
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome VIII

    Organism-specific databases

    CYGDiYHR146w.
    SGDiS000001189. CRP1.

    Subcellular locationi

    GO - Cellular componenti

    1. nucleus Source: SGD

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 465465Cruciform DNA-recognizing protein 1PRO_0000202923Add
    BLAST
    Chaini1 – 160160CRP1 short N-terminal subpeptidePRO_0000409594Add
    BLAST
    Chaini161 – 465305CRP1 short C-terminal subpeptidePRO_0000409595Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei153 – 1531Phosphoserine1 Publication
    Modified residuei156 – 1561Phosphoserine1 Publication
    Modified residuei182 – 1821Phosphothreonine1 Publication
    Modified residuei271 – 2711Phosphoserine2 Publications
    Modified residuei295 – 2951Phosphothreonine2 Publications
    Modified residuei319 – 3191Phosphoserine1 Publication
    Modified residuei343 – 3431Phosphoserine1 Publication
    Modified residuei366 – 3661Phosphothreonine1 Publication
    Modified residuei394 – 3941Phosphoserine1 Publication
    Modified residuei440 – 4401Phosphoserine3 Publications

    Post-translational modificationi

    Cleaved in the vicinity of position 160 to give an X-DNA-binding N-terminal subpeptide and a non-DNA-binding C-terminal subpeptide.

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    MaxQBiP38845.
    PaxDbiP38845.
    PeptideAtlasiP38845.

    Expressioni

    Gene expression databases

    GenevestigatoriP38845.

    Interactioni

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    itself1EBI-24770,EBI-24770
    AGP1P253761EBI-24770,EBI-2357
    ALO1P547831EBI-24770,EBI-2519
    ARF1P110761EBI-24770,EBI-2816
    ARF3P409941EBI-24770,EBI-2824
    ATG21Q028871EBI-24770,EBI-2045775
    BAP2P380841EBI-24770,EBI-3414
    CAB5Q039411EBI-24770,EBI-22174
    CAN1P048171EBI-24770,EBI-3993
    CHO1P084561EBI-24770,EBI-14055
    COT1P327981EBI-24770,EBI-5006
    DFG5Q050311EBI-24770,EBI-27512
    DIP5P533881EBI-24770,EBI-5904
    EMP47P435551EBI-24770,EBI-6439
    ENA2Q018961EBI-24770,EBI-2055692
    ERG1P324761EBI-24770,EBI-6545
    ERG25P530451EBI-24770,EBI-6506
    ERP1Q053591EBI-24770,EBI-6581
    ERV25P548371EBI-24770,EBI-6642
    FAA3P390021EBI-24770,EBI-10089
    FMP42Q049911EBI-24770,EBI-27478
    FMP45Q076511EBI-24770,EBI-2051056
    FTH1P383101EBI-24770,EBI-20959
    FTR1P400881EBI-24770,EBI-7138
    GAP1P191451EBI-24770,EBI-7314
    GPX2P381431EBI-24770,EBI-7863
    GTT1P405821EBI-24770,EBI-7941
    HNM1P198071EBI-24770,EBI-8409
    HSP30P256191EBI-24770,EBI-8563
    HXT1P324651EBI-24770,EBI-8759
    HXT3P324661EBI-24770,EBI-8770
    HXT5P386951EBI-24770,EBI-8778
    HXT7P390041EBI-24770,EBI-8790
    ITR1P306051EBI-24770,EBI-2050956
    MCD4P360511EBI-24770,EBI-2070979
    MSC3Q058121EBI-24770,EBI-33150
    MUP1P502761EBI-24770,EBI-11624
    ORM2Q061441EBI-24770,EBI-34916
    PDR12Q027851EBI-24770,EBI-13065
    PDR5P333021EBI-24770,EBI-13038
    PEX11Q124621EBI-24770,EBI-13198
    PIS1P061971EBI-24770,EBI-13458
    PLM2Q043831EBI-24770,EBI-2079237
    PMC1P389291EBI-24770,EBI-3097
    PMP2P409751EBI-24770,EBI-2043041
    PMP3P872841EBI-24770,EBI-13555
    QDR2P404741EBI-24770,EBI-25203
    QDR3P382271EBI-24770,EBI-21433
    RIM1P324451EBI-24770,EBI-15206
    SAC1P323681EBI-24770,EBI-16210
    SCS7Q035291EBI-24770,EBI-16747
    SDS23P531721EBI-24770,EBI-23782
    SEC4P075601EBI-24770,EBI-16858
    SFK1P357351EBI-24770,EBI-26678
    SNA3P143591EBI-24770,EBI-26122
    SNA4Q075491EBI-24770,EBI-22078
    SNQ2P325681EBI-24770,EBI-17590
    SPC1P469651EBI-24770,EBI-17823
    SPF1P399861EBI-24770,EBI-3128
    SWP1Q027951EBI-24770,EBI-12666
    TMS1Q121161EBI-24770,EBI-19306
    TPO1Q078241EBI-24770,EBI-38106
    TPO2P532831EBI-24770,EBI-2044753
    TPO3Q064511EBI-24770,EBI-34275
    VHS3Q084381EBI-24770,EBI-30482
    VHT1P532411EBI-24770,EBI-20297
    VMA21P418061EBI-24770,EBI-20323
    VMA6P323661EBI-24770,EBI-20201
    VPH1P325631EBI-24770,EBI-20455
    YBL039W-BP0C2681EBI-24770,EBI-2044092
    YCK3P399621EBI-24770,EBI-4740
    YDR034W-BQ6Q5X21EBI-24770,EBI-2043695
    YOP1Q124021EBI-24770,EBI-37092
    YPT1P011231EBI-24770,EBI-29496
    YVH1Q022561EBI-24770,EBI-14322

    Protein-protein interaction databases

    BioGridi36580. 32 interactions.
    IntActiP38845. 20 interactions.
    MINTiMINT-2734769.
    STRINGi4932.YHR146W.

    Structurei

    3D structure databases

    ProteinModelPortaliP38845.
    SMRiP38845. Positions 9-93.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni160 – 1612X-DNA-binding

    Sequence similaritiesi

    Belongs to the CRP1/MDG1 family.Curated

    Phylogenomic databases

    eggNOGiNOG12793.
    GeneTreeiENSGT00530000066262.
    OMAiVEIISAV.
    OrthoDBiEOG73V6W7.

    Family and domain databases

    InterProiIPR014756. Ig_E-set.
    [Graphical view]
    SUPFAMiSSF81296. SSF81296. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P38845-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSSELMFNYT FSWPAGPKDV ILTGTFDDWR GTLPLVKTAK GNFEITMPVK    50
    LANKDDTFQF KFIVDGVWCV SDSYKKEHVS EGIENNFLQI TDLVETQEVA 100
    GASRIPEAGG LLCGKPPRSA GPPSTSNRKK NKRNNKKRRS KLKKKSTKNN 150
    KKSNESLDDN EEEDGVTGTT TEDVTGTSRE ETPLAEPTNV SKEAPGNFHI 200
    LPIDQSADTT QSNGIIGGPG PVLVPNPGEI KEFTEIRDVD ARELNERLNK 250
    KEEVPEPVAG PIVESSVTEK SPALPQADDP IVETKEVAHN VQELTPQVEA 300
    VTPLINEPEP LPTPEAQISI PESSKVEPVE GSLQSKLVEK RESTEGVLDG 350
    SKKVENKAKK DEEVFTLDPI VNKAPKLPLT DEQTAEGRKS PAVSEEKEKK 400
    KKQEKGSKEV KRSETSKEKK PSAKEVKKQT VKAPKKQTAS PLSSSTEEPK 450
    KKKTGFFGKL KKLFK 465
    Length:465
    Mass (Da):51,116
    Last modified:February 1, 1995 - v1
    Checksum:i30880758F37991C7
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U10397 Genomic DNA. Translation: AAB68982.1.
    BK006934 Genomic DNA. Translation: DAA06839.1.
    PIRiS46759.
    RefSeqiNP_012016.1. NM_001179277.1.

    Genome annotation databases

    EnsemblFungiiYHR146W; YHR146W; YHR146W.
    GeneIDi856551.
    KEGGisce:YHR146W.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U10397 Genomic DNA. Translation: AAB68982.1 .
    BK006934 Genomic DNA. Translation: DAA06839.1 .
    PIRi S46759.
    RefSeqi NP_012016.1. NM_001179277.1.

    3D structure databases

    ProteinModelPortali P38845.
    SMRi P38845. Positions 9-93.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 36580. 32 interactions.
    IntActi P38845. 20 interactions.
    MINTi MINT-2734769.
    STRINGi 4932.YHR146W.

    Proteomic databases

    MaxQBi P38845.
    PaxDbi P38845.
    PeptideAtlasi P38845.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YHR146W ; YHR146W ; YHR146W .
    GeneIDi 856551.
    KEGGi sce:YHR146W.

    Organism-specific databases

    CYGDi YHR146w.
    SGDi S000001189. CRP1.

    Phylogenomic databases

    eggNOGi NOG12793.
    GeneTreei ENSGT00530000066262.
    OMAi VEIISAV.
    OrthoDBi EOG73V6W7.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-31181-MONOMER.

    Miscellaneous databases

    NextBioi 982357.

    Gene expression databases

    Genevestigatori P38845.

    Family and domain databases

    InterProi IPR014756. Ig_E-set.
    [Graphical view ]
    SUPFAMi SSF81296. SSF81296. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    2. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    3. "Crp1p, a new cruciform DNA-binding protein in the yeast Saccharomyces cerevisiae."
      Rass U., Kemper B.
      J. Mol. Biol. 323:685-700(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: CRUCIFORM DNA-BINDING, FUNCTION, DOMAIN, PROTEOLYTIC AUTOCLEAVAGE.
    4. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
    5. "Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
      Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
      J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-153; SER-156 AND SER-440, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Strain: ADR376.
    6. "Analysis of phosphorylation sites on proteins from Saccharomyces cerevisiae by electron transfer dissociation (ETD) mass spectrometry."
      Chi A., Huttenhower C., Geer L.Y., Coon J.J., Syka J.E.P., Bai D.L., Shabanowitz J., Burke D.J., Troyanskaya O.G., Hunt D.F.
      Proc. Natl. Acad. Sci. U.S.A. 104:2193-2198(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    7. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
      Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
      Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-271; THR-295; SER-319; SER-394 AND SER-440, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    8. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
      Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
      Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-182; SER-271; THR-295; SER-343; THR-366 AND SER-440, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiCRP1_YEAST
    AccessioniPrimary (citable) accession number: P38845
    Secondary accession number(s): D3DL95
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 1, 1995
    Last sequence update: February 1, 1995
    Last modified: October 1, 2014
    This is version 97 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 2990 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families
    2. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    3. Yeast chromosome VIII
      Yeast (Saccharomyces cerevisiae) chromosome VIII: entries and gene names

    External Data

    Dasty 3