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Protein

Cruciform DNA-recognizing protein 1

Gene

CRP1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Cruciform DNA-binding protein which exerts an enhancing effect on the cleavage of cruciform DNA (X-DNA) by endonuclease VII from bacteriophage T4.1 Publication

Miscellaneous

Present with 2990 molecules/cell in log phase SD medium.1 Publication

GO - Molecular functioni

  • DNA binding Source: SGD

Keywordsi

Molecular functionDNA-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-31181-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Cruciform DNA-recognizing protein 1
Cleaved into the following 2 chains:
Gene namesi
Name:CRP1
Ordered Locus Names:YHR146W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome VIII

Organism-specific databases

EuPathDBiFungiDB:YHR146W.
SGDiS000001189. CRP1.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002029231 – 465Cruciform DNA-recognizing protein 1Add BLAST465
ChainiPRO_00004095941 – 160CRP1 short N-terminal subpeptideAdd BLAST160
ChainiPRO_0000409595161 – 465CRP1 short C-terminal subpeptideAdd BLAST305

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei153PhosphoserineCombined sources1
Modified residuei156PhosphoserineCombined sources1
Modified residuei182PhosphothreonineCombined sources1
Modified residuei271PhosphoserineCombined sources1
Modified residuei295PhosphothreonineCombined sources1
Modified residuei319PhosphoserineCombined sources1
Modified residuei343PhosphoserineCombined sources1
Modified residuei366PhosphothreonineCombined sources1
Modified residuei394PhosphoserineCombined sources1
Modified residuei440PhosphoserineCombined sources1

Post-translational modificationi

Cleaved in the vicinity of position 160 to give an X-DNA-binding N-terminal subpeptide and a non-DNA-binding C-terminal subpeptide.

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP38845.
PRIDEiP38845.

PTM databases

iPTMnetiP38845.

Interactioni

Binary interactionsi

Show more details

Protein-protein interaction databases

BioGridi36580. 107 interactors.
IntActiP38845. 20 interactors.
MINTiMINT-2734769.
STRINGi4932.YHR146W.

Structurei

3D structure databases

ProteinModelPortaliP38845.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni160 – 161X-DNA-binding2

Sequence similaritiesi

Belongs to the CRP1/MDG1 family.Curated

Phylogenomic databases

GeneTreeiENSGT00530000066262.
InParanoidiP38845.
OMAiNNMIPES.
OrthoDBiEOG092C5OKR.

Family and domain databases

Gene3Di2.60.40.10. 1 hit.
InterProiView protein in InterPro
IPR032640. AMPK1_CBM.
IPR013783. Ig-like_fold.
IPR014756. Ig_E-set.
PfamiView protein in Pfam
PF16561. AMPK1_CBM. 1 hit.
SUPFAMiSSF81296. SSF81296. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P38845-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSSELMFNYT FSWPAGPKDV ILTGTFDDWR GTLPLVKTAK GNFEITMPVK
60 70 80 90 100
LANKDDTFQF KFIVDGVWCV SDSYKKEHVS EGIENNFLQI TDLVETQEVA
110 120 130 140 150
GASRIPEAGG LLCGKPPRSA GPPSTSNRKK NKRNNKKRRS KLKKKSTKNN
160 170 180 190 200
KKSNESLDDN EEEDGVTGTT TEDVTGTSRE ETPLAEPTNV SKEAPGNFHI
210 220 230 240 250
LPIDQSADTT QSNGIIGGPG PVLVPNPGEI KEFTEIRDVD ARELNERLNK
260 270 280 290 300
KEEVPEPVAG PIVESSVTEK SPALPQADDP IVETKEVAHN VQELTPQVEA
310 320 330 340 350
VTPLINEPEP LPTPEAQISI PESSKVEPVE GSLQSKLVEK RESTEGVLDG
360 370 380 390 400
SKKVENKAKK DEEVFTLDPI VNKAPKLPLT DEQTAEGRKS PAVSEEKEKK
410 420 430 440 450
KKQEKGSKEV KRSETSKEKK PSAKEVKKQT VKAPKKQTAS PLSSSTEEPK
460
KKKTGFFGKL KKLFK
Length:465
Mass (Da):51,116
Last modified:February 1, 1995 - v1
Checksum:i30880758F37991C7
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U10397 Genomic DNA. Translation: AAB68982.1.
BK006934 Genomic DNA. Translation: DAA06839.1.
PIRiS46759.
RefSeqiNP_012016.1. NM_001179277.1.

Genome annotation databases

EnsemblFungiiYHR146W; YHR146W; YHR146W.
GeneIDi856551.
KEGGisce:YHR146W.

Similar proteinsi

Entry informationi

Entry nameiCRP1_YEAST
AccessioniPrimary (citable) accession number: P38845
Secondary accession number(s): D3DL95
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: February 1, 1995
Last modified: September 27, 2017
This is version 120 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome VIII
    Yeast (Saccharomyces cerevisiae) chromosome VIII: entries and gene names