P38843 (CHS7_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Chitin synthase export chaperone

Gene names

Name:	CHS7
Ordered Locus Names:	YHR142W

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	316 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Chaperone required for the export of the chitin synthase CHS3 from the endoplasmic reticulum. Ref.4 Ref.5 Ref.6 Ref.7
Subunit structure	Interacts with CHS3. Ref.8
Subcellular location	Endoplasmic reticulum membrane; Multi-pass membrane protein Ref.4.
Sequence similarities	Belongs to the CHS7 family.

Ontologies

Keywords
Biological process	Cell wall biogenesis/degradation Protein transport Transport
Cellular component	Endoplasmic reticulum Membrane
Domain	Transmembrane Transmembrane helix
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	ER to Golgi vesicle-mediated transport Inferred from direct assay Ref.4. Source: SGD cellular cell wall organization Inferred from electronic annotation. Source: UniProtKB-KW fungal-type cell wall chitin biosynthetic process Inferred from mutant phenotype Ref.4. Source: SGD protein folding Inferred from mutant phenotype Ref.7. Source: SGD protein transport Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	endoplasmic reticulum membrane Inferred from direct assay Ref.4. Source: SGD integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	unfolded protein binding Inferred from mutant phenotype Ref.7. Source: SGD
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
AGP1	P25376	1	EBI-24756,EBI-2357
CHS3	P29465	1	EBI-24756,EBI-4632
GAP1	P19145	1	EBI-24756,EBI-7314
KEX1	P09620	1	EBI-24756,EBI-9653
OST1	P41543	1	EBI-24756,EBI-12651
PMP2	P40975	1	EBI-24756,EBI-2043041
SOP4	P39543	1	EBI-24756,EBI-26193
SPF1	P39986	1	EBI-24756,EBI-3128

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 316	316	Chitin synthase export chaperone		PRO_0000202922
Regions
	Transmembrane	62 – 82	21	Helical; Potential
	Transmembrane	99 – 119	21	Helical; Potential
	Transmembrane	130 – 150	21	Helical; Potential
	Transmembrane	170 – 190	21	Helical; Potential
	Transmembrane	200 – 220	21	Helical; Potential
	Transmembrane	233 – 253	21	Helical; Potential
	Transmembrane	266 – 286	21	Helical; Potential

Sequences

	Sequence		Length	Mass (Da)
	P38843 [UniParc]. Last modified February 1, 1995. Version 1. Checksum: 9DA1EAFA181B2409	FASTA	316	34,898
	10 20 30 40 50 60 MAFSDFAAIC SKTPLPLCSV IKSKTHLILS NSTIIHDFDP LNLNVGVLPR CYARSIDLAN 70 80 90 100 110 120 TVIFDVGNAF INIGALGVIL IILYNIRQKY TAIGRSEYLY FFQLTLLLII FTLVVDCGVS 130 140 150 160 170 180 PPGSGSYPYF VAIQIGLAGA CCWALLIIGF LGFNLWEDGT TKSMLLVRGT SMLGFIANFL 190 200 210 220 230 240 ASILTFKAWI TDHKVATMNA SGMIVVVYII NAIFLFVFVI CQLLVSLLVV RNLWVTGAIF 250 260 270 280 290 300 LGLFFFVAGQ VLVYAFSTQI CEGFKHYLDG LFFGSICNVF TLMMVYKTWD MTTDDDLEFG 310 VSVSKDGDVV YDNGFM

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Complete nucleotide sequence of Saccharomyces cerevisiae chromosome VIII." Johnston M., Andrews S., Brinkman R., Cooper J., Ding H., Dover J., Du Z., Favello A., Fulton L., Gattung S., Geisel C., Kirsten J., Kucaba T., Hillier L.W., Jier M., Johnston L., Langston Y., Latreille P. , Louis E.J., Macri C., Mardis E., Menezes S., Mouser L., Nhan M., Rifkin L., Riles L., St Peter H., Trevaskis E., Vaughan K., Vignati D., Wilcox L., Wohldman P., Waterston R., Wilson R., Vaudin M. Science 265:2077-2082(1994) [PubMed: 8091229] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[2]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[3]	"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae." Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J. Genome Res. 17:536-543(2007) [PubMed: 17322287] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[4]	"Chs7p, a new protein involved in the control of protein export from the endoplasmic reticulum that is specifically engaged in the regulation of chitin synthesis in Saccharomyces cerevisiae." Trilla J.A., Duran A., Roncero C. J. Cell Biol. 145:1153-1163(1999) [PubMed: 10366589] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION.
[5]	"Involvement of GFA1, which encodes glutamine-fructose-6-phosphate amidotransferase, in the activation of the chitin synthesis pathway in response to cell-wall defects in Saccharomyces cerevisiae." Lagorce A., Le Berre-Anton V., Aguilar-Uscanga B., Martin-Yken H., Dagkessamanskaia A., Francois J. Eur. J. Biochem. 269:1697-1707(2002) [PubMed: 11895440] [Abstract] Cited for: FUNCTION.
[6]	"Maintenance of cell integrity in the gas1 mutant of Saccharomyces cerevisiae requires the Chs3p-targeting and activation pathway and involves an unusual Chs3p localization." Carotti C., Ferrario L., Roncero C., Valdivieso M.-H., Duran A., Popolo L. Yeast 19:1113-1124(2002) [PubMed: 12237852] [Abstract] Cited for: FUNCTION.
[7]	"Specialized membrane-localized chaperones prevent aggregation of polytopic proteins in the ER." Kota J., Ljungdahl P.O. J. Cell Biol. 168:79-88(2005) [PubMed: 15623581] [Abstract] Cited for: FUNCTION.
[8]	"Palmitoylation by the DHHC protein Pfa4 regulates the ER exit of Chs3." Lam K.K.Y., Davey M., Sun B., Roth A.F., Davis N.G., Conibear E. J. Cell Biol. 174:19-25(2006) [PubMed: 16818716] [Abstract] Cited for: INTERACTION WITH CHS3.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U10397 Genomic DNA. Translation: AAB68984.1. AY692978 Genomic DNA. Translation: AAT92997.1. BK006934 Genomic DNA. Translation: DAA06835.1.
PIR	S46761.
RefSeq	NP_012011.1. NM_001179272.1.
3D structure databases
ProteinModelPortal	P38843.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-5238N.
IntAct	P38843. 8 interactions.
MINT	MINT-491582.
STRING	P38843.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YHR142W; YHR142W; YHR142W.
GeneID	856545.
KEGG	sce:YHR142W.
NMPDR	fig|4932.3.peg.3175.
Organism-specific databases
CYGD	YHR142w.
SGD	S000001184. CHS7.
Phylogenomic databases
eggNOG	fuNOG04121.
GeneTree	EFGT00050000003557.
HOGENOM	HBG333395.
OMA	EDLEFSV.
OrthoDB	EOG47WRXN.
Gene expression databases
ArrayExpress	P38843.
Genevestigator	P38843.
GermOnline	YHR142W. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR022057. Chs3p. [Graphical view]
Pfam	PF12271. Chs3p. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	982343.

Entry information

Entry name

CHS7_YEAST

Accession

Primary (citable) accession number: P38843
Secondary accession number(s): D3DL91

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1995
Last sequence update:	February 1, 1995
Last modified:	December 14, 2011
This is version 80 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program