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Protein

Aromatic amino acid aminotransferase 2

Gene

ARO9

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

General aromatic amino acid transaminase involved in several otherwise unrelated metabolic pathways. Mainly involved in tryptophan degradation. Active with phenylalanine, tyrosine and tryptophan as amino donors and with phenylpyruvate, hydroxyphenylpyruvate and pyruvate as amino acceptors. Does not accept glutamate or 2-oxoglutarate as substrates. Also active with methionine, leucine, glutamine and kynurenine. Catalyzes the formation of methionine from 2-keto-4-methylthiobutyrate (KMTB) in the methionine salvage pathway primarily using aromatic amino acids (tyrosine, phenylalanine and tryptophan) as the amino donors. Catalyzes the irreversible transamination of the L-tryptophan metabolite L-kynurenine to form kynurenic acid (KA) with pyruvate as amino acceptor.4 Publications

Catalytic activityi

An aromatic amino acid + 2-oxoglutarate = an aromatic oxo acid + L-glutamate.
An aromatic amino acid + 4-(methylthio)-2-oxobutanoate = an aromatic oxo acid + L-methionine.
L-kynurenine + 2-oxoglutarate = 4-(2-aminophenyl)-2,4-dioxobutanoate + L-glutamate.

Cofactori

Pathwayi: L-methionine biosynthesis via salvage pathway

This protein is involved in step 6 of the subpathway that synthesizes L-methionine from S-methyl-5-thio-alpha-D-ribose 1-phosphate.
Proteins known to be involved in the 6 steps of the subpathway in this organism are:
  1. Methylthioribose-1-phosphate isomerase (MRI1)
  2. Methylthioribulose-1-phosphate dehydratase (MDE1)
  3. Enolase-phosphatase E1 (UTR4)
  4. Enolase-phosphatase E1 (UTR4)
  5. 1,2-dihydroxy-3-keto-5-methylthiopentene dioxygenase (ADI1)
  6. Aromatic amino acid aminotransferase 2 (ARO9), Branched-chain-amino-acid aminotransferase, mitochondrial (BAT1), Branched-chain-amino-acid aminotransferase, cytosolic (BAT2), Aromatic/aminoadipate aminotransferase 1 (ARO8)
This subpathway is part of the pathway L-methionine biosynthesis via salvage pathway, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-methionine from S-methyl-5-thio-alpha-D-ribose 1-phosphate, the pathway L-methionine biosynthesis via salvage pathway and in Amino-acid biosynthesis.

Pathwayi: L-kynurenine degradation

This protein is involved in step 1 of the subpathway that synthesizes kynurenate from L-kynurenine.
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. Aromatic amino acid aminotransferase 2 (ARO9), Probable kynurenine--oxoglutarate transaminase BNA3 (BNA3)
  2. no protein annotated in this organism
This subpathway is part of the pathway L-kynurenine degradation, which is itself part of Amino-acid degradation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes kynurenate from L-kynurenine, the pathway L-kynurenine degradation and in Amino-acid degradation.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Aminotransferase, Transferase

Keywords - Ligandi

Pyridoxal phosphate

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-11842.
YEAST:YHR137W-MONOMER.
BRENDAi2.6.1.57. 984.
ReactomeiR-SCE-71064. Lysine catabolism.
R-SCE-71240. Tryptophan catabolism.
UniPathwayiUPA00334; UER00726.
UPA00904; UER00879.

Names & Taxonomyi

Protein namesi
Recommended name:
Aromatic amino acid aminotransferase 2 (EC:2.6.1.57)
Alternative name(s):
Aromatic amino acid aminotransferase II
Aromatic amino acid-requiring protein 9
Kynurenine aminotransferase I (EC:2.6.1.7)
Short name:
KAT I
Gene namesi
Name:ARO9
Ordered Locus Names:YHR137W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome VIII

Organism-specific databases

EuPathDBiFungiDB:YHR137W.
SGDiS000001179. ARO9.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 513513Aromatic amino acid aminotransferase 2PRO_0000064679Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei90 – 901PhosphoserineCombined sources
Modified residuei92 – 921PhosphoserineCombined sources

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP38840.
PRIDEiP38840.

PTM databases

iPTMnetiP38840.

Expressioni

Inductioni

By aromatic amino acids in the growth medium. Expression also induced in ARO8 mutants grown on minimal ammonia medium.2 Publications

Interactioni

Protein-protein interaction databases

BioGridi36570. 20 interactions.
DIPiDIP-4942N.
IntActiP38840. 3 interactions.
MINTiMINT-475675.

Structurei

3D structure databases

ProteinModelPortaliP38840.
SMRiP38840. Positions 41-506.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

GeneTreeiENSGT00390000004594.
HOGENOMiHOG000223057.
InParanoidiP38840.
KOiK05821.
OMAiETICDES.
OrthoDBiEOG092C1J5R.

Family and domain databases

Gene3Di3.40.640.10. 1 hit.
3.90.1150.10. 1 hit.
InterProiIPR004839. Aminotransferase_I/II.
IPR015424. PyrdxlP-dep_Trfase.
IPR015421. PyrdxlP-dep_Trfase_major_sub1.
IPR015422. PyrdxlP-dep_Trfase_major_sub2.
[Graphical view]
PfamiPF00155. Aminotran_1_2. 1 hit.
[Graphical view]
SUPFAMiSSF53383. SSF53383. 2 hits.

Sequencei

Sequence statusi: Complete.

P38840-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTAGSAPPVD YTSLKKNFQP FLSRRVENRS LKSFWDASDI SDDVIELAGG
60 70 80 90 100
MPNERFFPIE SMDLKISKVP FNDNPKWHNS FTTAHLDLGS PSELPIARSF
110 120 130 140 150
QYAETKGLPP LLHFVKDFVS RINRPAFSDE TESNWDVILS GGSNDSMFKV
160 170 180 190 200
FETICDESTT VMIEEFTFTP AMSNVEATGA KVIPIKMNLT FDRESQGIDV
210 220 230 240 250
EYLTQLLDNW STGPYKDLNK PRVLYTIATG QNPTGMSVPQ WKREKIYQLA
260 270 280 290 300
QRHDFLIVED DPYGYLYFPS YNPQEPLENP YHSSDLTTER YLNDFLMKSF
310 320 330 340 350
LTLDTDARVI RLETFSKIFA PGLRLSFIVA NKFLLQKILD LADITTRAPS
360 370 380 390 400
GTSQAIVYST IKAMAESNLS SSLSMKEAMF EGWIRWIMQI ASKYNHRKNL
410 420 430 440 450
TLKALYETES YQAGQFTVME PSAGMFIIIK INWGNFDRPD DLPQQMDILD
460 470 480 490 500
KFLLKNGVKV VLGYKMAVCP NYSKQNSDFL RLTIAYARDD DQLIEASKRI
510
GSGIKEFFDN YKS
Length:513
Mass (Da):58,528
Last modified:October 5, 2010 - v3
Checksum:i8DA49174D59C8859
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti460 – 4601V → L in CAA73950 (PubMed:9491083).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y13625 Genomic DNA. Translation: CAA73950.1.
U10398 Genomic DNA. Translation: AAB68403.1.
BK006934 Genomic DNA. Translation: DAA06830.1.
PIRiS48981.
RefSeqiNP_012005.1. NM_001179267.1.

Genome annotation databases

EnsemblFungiiYHR137W; YHR137W; YHR137W.
GeneIDi856539.
KEGGisce:YHR137W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y13625 Genomic DNA. Translation: CAA73950.1.
U10398 Genomic DNA. Translation: AAB68403.1.
BK006934 Genomic DNA. Translation: DAA06830.1.
PIRiS48981.
RefSeqiNP_012005.1. NM_001179267.1.

3D structure databases

ProteinModelPortaliP38840.
SMRiP38840. Positions 41-506.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi36570. 20 interactions.
DIPiDIP-4942N.
IntActiP38840. 3 interactions.
MINTiMINT-475675.

PTM databases

iPTMnetiP38840.

Proteomic databases

MaxQBiP38840.
PRIDEiP38840.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYHR137W; YHR137W; YHR137W.
GeneIDi856539.
KEGGisce:YHR137W.

Organism-specific databases

EuPathDBiFungiDB:YHR137W.
SGDiS000001179. ARO9.

Phylogenomic databases

GeneTreeiENSGT00390000004594.
HOGENOMiHOG000223057.
InParanoidiP38840.
KOiK05821.
OMAiETICDES.
OrthoDBiEOG092C1J5R.

Enzyme and pathway databases

UniPathwayiUPA00334; UER00726.
UPA00904; UER00879.
BioCyciMetaCyc:MONOMER-11842.
YEAST:YHR137W-MONOMER.
BRENDAi2.6.1.57. 984.
ReactomeiR-SCE-71064. Lysine catabolism.
R-SCE-71240. Tryptophan catabolism.

Miscellaneous databases

PROiP38840.

Family and domain databases

Gene3Di3.40.640.10. 1 hit.
3.90.1150.10. 1 hit.
InterProiIPR004839. Aminotransferase_I/II.
IPR015424. PyrdxlP-dep_Trfase.
IPR015421. PyrdxlP-dep_Trfase_major_sub1.
IPR015422. PyrdxlP-dep_Trfase_major_sub2.
[Graphical view]
PfamiPF00155. Aminotran_1_2. 1 hit.
[Graphical view]
SUPFAMiSSF53383. SSF53383. 2 hits.
ProtoNetiSearch...

Entry informationi

Entry nameiARO9_YEAST
AccessioniPrimary (citable) accession number: P38840
Secondary accession number(s): D3DL86, P87291
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: October 5, 2010
Last modified: September 7, 2016
This is version 124 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 7700 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome VIII
    Yeast (Saccharomyces cerevisiae) chromosome VIII: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.