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Protein

DNA-dependent metalloprotease WSS1

Gene

WSS1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Metalloendopeptidase that acts selectively on DNA-binding proteins. DNA is needed to bring the protease and substrates together to enable proteolysis. Involved in the repair of toxic DNA-protein cross-links (DPCs) such as covalently trapped topoisomerase 1 (TOP1) adducts on DNA lesions or DPCs induced by reactive compounds such as formaldehyde. Involved in DNA damage response and processing of stalled or collapsed replication forks by removing the covalently trapped TOP1 from chromatin. DPC proteolysis enables the repair of the lesions via downstream DNA repair pathways. May be recruited to DPCs via the SUMOylation of substrate proteins at damaged DNA sites.6 Publications

Enzyme regulationi

Inactivated by EDTA.1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi115 – 1151Zinc; catalyticPROSITE-ProRule annotation
Active sitei116 – 1161PROSITE-ProRule annotation
Metal bindingi119 – 1191Zinc; catalyticPROSITE-ProRule annotation
Metal bindingi125 – 1251Zinc; catalyticPROSITE-ProRule annotation

GO - Molecular functioni

  • metal ion binding Source: UniProtKB-KW
  • metalloendopeptidase activity Source: SGD

GO - Biological processi

  • cellular response to DNA damage stimulus Source: SGD
  • protein sumoylation Source: SGD
  • response to UV-B Source: SGD
  • response to UV-C Source: SGD
  • translesion synthesis Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Metalloprotease, Protease

Keywords - Biological processi

DNA damage

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

BioCyciYEAST:G3O-31172-MONOMER.

Protein family/group databases

MEROPSiM80.001.

Names & Taxonomyi

Protein namesi
Recommended name:
DNA-dependent metalloprotease WSS1 (EC:3.4.24.-)
Alternative name(s):
DNA damage response protein WSS1
SUMO-dependent isopeptidase WSS1
Weak suppressor of SMT3 protein 1
Gene namesi
Name:WSS1
Ordered Locus Names:YHR134W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome VIII

Organism-specific databases

EuPathDBiFungiDB:YHR134W.
SGDiS000001176. WSS1.

Subcellular locationi

  • Nucleus 1 Publication

  • Note: Present in a single sharp spot near the nuclear membrane, distinct from the spindle pole bodies and nucleolus. In dividing cells, the spot is exclusively present in the mother cell.

GO - Cellular componenti

  • nuclear envelope Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Disruption phenotypei

Accumulates DPCs and exhibits gross chromosomal rearrangements.1 Publication

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi115 – 1151H → A in wss1-pd; loss of function; when asscoiated with A-119. 1 Publication
Mutagenesisi116 – 1161E → Q: Loss of function. 1 Publication
Mutagenesisi119 – 1191H → A in wss1-pd; loss of function; when asscoiated with A-115. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 269269DNA-dependent metalloprotease WSS1PRO_0000202919Add
BLAST

Proteomic databases

TopDownProteomicsiP38838.

Interactioni

Subunit structurei

Binds to DNA. Interacts with CDC48 and SMT3. Interacts with PSY2 and TOF1.3 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
UBE2IP632793EBI-24737,EBI-80168From a different organism.

Protein-protein interaction databases

BioGridi36567. 66 interactions.
DIPiDIP-1600N.
IntActiP38838. 4 interactions.
MINTiMINT-411535.

Structurei

3D structure databases

ProteinModelPortaliP38838.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini20 – 221202WLMPROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni161 – 20848Required and sufficient for binding to DNAAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi152 – 1609SHP box
Motifi209 – 21911VCP-interaction motif (VIM)Add
BLAST
Motifi247 – 2548SUMO interaction motif 1 (SIM)
Motifi266 – 2694SUMO interaction motif 2 (SIM)

Domaini

The SHP box and the VCP-interaction motif (VIM) are important both together for binding to CDC48. The 2 SUMO interaction motifs (SIM) are each important for binding to SMT3(SUMO).1 Publication

Sequence similaritiesi

Contains 1 WLM domain.PROSITE-ProRule annotation

Phylogenomic databases

HOGENOMiHOG000093863.
InParanoidiP38838.
OMAiIMGTMLH.
OrthoDBiEOG7HQNJH.

Family and domain databases

InterProiIPR013536. WLM_dom.
[Graphical view]
PfamiPF08325. WLM. 1 hit.
[Graphical view]
PROSITEiPS51397. WLM. 1 hit.
PS00142. ZINC_PROTEASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P38838-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKTEGIKSPS AKYHDMAGSQ RIPHKNPHIQ KVAVLQSKPN KEDALNLIKE
60 70 80 90 100
IAHKVSYLMK ENHFKVTNLV EFYPRDQRLL GMNVNHGSKI MLRLRCSTDE
110 120 130 140 150
FQFLPMECIM GTMLHELTHN LFGPHDKKFY NKLDELIGRQ WVIEQRGLYD
160 170 180 190 200
TFLGNGQRLG GRANLRSNRY PMTGISTNTG IVRKRGKGVK LGSLHPEGIS
210 220 230 240 250
SIDRGNSPRE LAAFAAERRY RDDRWCGETK NNKDQIISDN ISSSLEVVIL
260
DDDDEVLPGD TLIEVIDLT
Length:269
Mass (Da):30,627
Last modified:February 1, 1995 - v1
Checksum:i09DC0854537F9D02
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U10398 Genomic DNA. Translation: AAB68404.1.
BK006934 Genomic DNA. Translation: DAA06827.1.
PIRiS48978.
RefSeqiNP_012002.1. NM_001179264.1.

Genome annotation databases

EnsemblFungiiYHR134W; YHR134W; YHR134W.
GeneIDi856536.
KEGGisce:YHR134W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U10398 Genomic DNA. Translation: AAB68404.1.
BK006934 Genomic DNA. Translation: DAA06827.1.
PIRiS48978.
RefSeqiNP_012002.1. NM_001179264.1.

3D structure databases

ProteinModelPortaliP38838.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi36567. 66 interactions.
DIPiDIP-1600N.
IntActiP38838. 4 interactions.
MINTiMINT-411535.

Protein family/group databases

MEROPSiM80.001.

Proteomic databases

TopDownProteomicsiP38838.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYHR134W; YHR134W; YHR134W.
GeneIDi856536.
KEGGisce:YHR134W.

Organism-specific databases

EuPathDBiFungiDB:YHR134W.
SGDiS000001176. WSS1.

Phylogenomic databases

HOGENOMiHOG000093863.
InParanoidiP38838.
OMAiIMGTMLH.
OrthoDBiEOG7HQNJH.

Enzyme and pathway databases

BioCyciYEAST:G3O-31172-MONOMER.

Miscellaneous databases

NextBioi982319.
PROiP38838.

Family and domain databases

InterProiIPR013536. WLM_dom.
[Graphical view]
PfamiPF08325. WLM. 1 hit.
[Graphical view]
PROSITEiPS51397. WLM. 1 hit.
PS00142. ZINC_PROTEASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  2. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  3. "Genes involved in sister chromatid separation and segregation in the budding yeast Saccharomyces cerevisiae."
    Biggins S., Bhalla N., Chang A., Smith D.L., Murray A.W.
    Genetics 159:453-470(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  4. "A genome-wide screen in Saccharomyces cerevisiae for genes affecting UV radiation sensitivity."
    Birrell G.W., Giaever G., Chu A.M., Davis R.W., Brown J.M.
    Proc. Natl. Acad. Sci. U.S.A. 98:12608-12613(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  5. "Coordinated functions of WSS1, PSY2 and TOF1 in the DNA damage response."
    O'Neill B.M., Hanway D., Winzeler E.A., Romesberg F.E.
    Nucleic Acids Res. 32:6519-6530(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH PSY2 AND TOF1.
  6. "Genome-wide analysis of Rad52 foci reveals diverse mechanisms impacting recombination."
    Alvaro D., Lisby M., Rothstein R.
    PLoS Genet. 3:E228-E228(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  7. "The Saccharomyces cerevisiae Wss1 protein is only present in mother cells."
    van Heusden G.P.H., Steensma H.Y.
    FEMS Microbiol. Lett. 282:100-104(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION.
  8. "Wss1 is a SUMO-dependent isopeptidase that interacts genetically with the Slx5-Slx8 SUMO-targeted ubiquitin ligase."
    Mullen J.R., Chen C.F., Brill S.J.
    Mol. Cell. Biol. 30:3737-3748(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, MUTAGENESIS OF HIS-115 AND HIS-119, INTERACTION WITH SMT3.
  9. "A DNA-dependent protease involved in DNA-protein crosslink repair."
    Stingele J., Schwarz M.S., Bloemeke N., Wolf P.G., Jentsch S.
    Cell 158:327-338(2014) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, ENZYME REGULATION, MUTAGENESIS OF GLU-116, INTERACTION WITH CDC48 AND SMT3, DNA-BINDING, DOMAIN, DISRUPTION PHENOTYPE.

Entry informationi

Entry nameiWSS1_YEAST
AccessioniPrimary (citable) accession number: P38838
Secondary accession number(s): D3DL83
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: February 1, 1995
Last modified: May 11, 2016
This is version 104 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Caution

Was initially reported to be a SUMO-dependent isopeptidase (PubMed:20516210), but this activity could not be confirmed (PubMed:24998930).2 Publications

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome VIII
    Yeast (Saccharomyces cerevisiae) chromosome VIII: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.