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Protein

Protein NSG1

Gene

NSG1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Stabilizes the HMG-CoA reductase HMG2 by preventing its HRD1-dependent degradation. Binds directly to the sterol-sensing domain (SSD)-containing transmembrane region of HMG2, promoting its folding to protect it from degradation.1 Publication

GO - Molecular functioni

  • unfolded protein binding Source: SGD

GO - Biological processi

  • sterol biosynthetic process Source: SGD
Complete GO annotation...

Enzyme and pathway databases

BioCyciYEAST:G3O-31171-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Protein NSG1
Alternative name(s):
INSIG homolog 1
Gene namesi
Name:NSG1
Ordered Locus Names:YHR133C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome VIII

Organism-specific databases

EuPathDBiFungiDB:YHR133C.
SGDiS000001175. NSG1.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 9696CytoplasmicSequence analysisAdd
BLAST
Transmembranei97 – 11721HelicalSequence analysisAdd
BLAST
Topological domaini118 – 15639Perinuclear spaceSequence analysisAdd
BLAST
Transmembranei157 – 17721HelicalSequence analysisAdd
BLAST
Topological domaini178 – 23053CytoplasmicSequence analysisAdd
BLAST
Transmembranei231 – 25121HelicalSequence analysisAdd
BLAST
Topological domaini252 – 26110Perinuclear spaceSequence analysis
Transmembranei262 – 28221HelicalSequence analysisAdd
BLAST
Topological domaini283 – 2919CytoplasmicSequence analysis

GO - Cellular componenti

  • cytosol Source: SGD
  • endoplasmic reticulum Source: SGD
  • endoplasmic reticulum membrane Source: UniProtKB-SubCell
  • integral component of membrane Source: UniProtKB-KW
  • nucleus Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 291291Protein NSG1PRO_0000202918Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei39 – 391PhosphoserineCombined sources
Modified residuei76 – 761PhosphoserineCombined sources

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP38837.

PTM databases

iPTMnetiP38837.

Interactioni

GO - Molecular functioni

  • unfolded protein binding Source: SGD

Protein-protein interaction databases

BioGridi36566. 42 interactions.
DIPiDIP-2775N.
IntActiP38837. 9 interactions.
MINTiMINT-480589.

Structurei

3D structure databases

ProteinModelPortaliP38837.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

To yeast YNL156c.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

GeneTreeiENSGT00530000066435.
HOGENOMiHOG000113862.
InParanoidiP38837.
OrthoDBiEOG092C4AO9.

Family and domain databases

InterProiIPR025929. INSIG_fam.
[Graphical view]
PfamiPF07281. INSIG. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P38837-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGKKKSKNQL NTGGVPNGVH NTKKEAALPP LGNKLGSASF TAINTLTKPA
60 70 80 90 100
LFSFYDDDIT KNEGNVYDKA LLSNASQLEM VPPSATARHE RSLYAKIINT
110 120 130 140 150
IAAFFILFIA GILFPMISEC LFDNDQLAKG DIVSFLKHGI EIKNKIVAEP
160 170 180 190 200
DMVPDWAVFG TEGVIFGSIV PFIDSFVRYQ HQPKTRSSVY KNTLGSFIRC
210 220 230 240 250
ANTLLGLIFG IRKLEWSSSL QAAGAWSLLN IVLWLFFDGT LTVFFPGLVI
260 270 280 290
GALSAFTCSQ CFSQLSLALY FIDFYFFGFL MFSKLGRYLF N
Length:291
Mass (Da):32,214
Last modified:February 1, 1995 - v1
Checksum:iD0A634938AAD118E
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti8 – 81N → T in AAS56435 (PubMed:17322287).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U10398 Genomic DNA. Translation: AAB68416.1.
AY558109 Genomic DNA. Translation: AAS56435.1.
BK006934 Genomic DNA. Translation: DAA06826.1.
PIRiS48977.
RefSeqiNP_012001.1. NM_001179263.1.

Genome annotation databases

EnsemblFungiiYHR133C; YHR133C; YHR133C.
GeneIDi856535.
KEGGisce:YHR133C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U10398 Genomic DNA. Translation: AAB68416.1.
AY558109 Genomic DNA. Translation: AAS56435.1.
BK006934 Genomic DNA. Translation: DAA06826.1.
PIRiS48977.
RefSeqiNP_012001.1. NM_001179263.1.

3D structure databases

ProteinModelPortaliP38837.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi36566. 42 interactions.
DIPiDIP-2775N.
IntActiP38837. 9 interactions.
MINTiMINT-480589.

PTM databases

iPTMnetiP38837.

Proteomic databases

MaxQBiP38837.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYHR133C; YHR133C; YHR133C.
GeneIDi856535.
KEGGisce:YHR133C.

Organism-specific databases

EuPathDBiFungiDB:YHR133C.
SGDiS000001175. NSG1.

Phylogenomic databases

GeneTreeiENSGT00530000066435.
HOGENOMiHOG000113862.
InParanoidiP38837.
OrthoDBiEOG092C4AO9.

Enzyme and pathway databases

BioCyciYEAST:G3O-31171-MONOMER.

Miscellaneous databases

PROiP38837.

Family and domain databases

InterProiIPR025929. INSIG_fam.
[Graphical view]
PfamiPF07281. INSIG. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiNSG1_YEAST
AccessioniPrimary (citable) accession number: P38837
Secondary accession number(s): D3DL82, Q6Q5G1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: February 1, 1995
Last modified: September 7, 2016
This is version 111 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 1900 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  2. Yeast chromosome VIII
    Yeast (Saccharomyces cerevisiae) chromosome VIII: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.