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Protein

Protein NSG1

Gene

NSG1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Stabilizes the HMG-CoA reductase HMG2 by preventing its HRD1-dependent degradation. Binds directly to the sterol-sensing domain (SSD)-containing transmembrane region of HMG2, promoting its folding to protect it from degradation.1 Publication

Miscellaneous

Present with 1900 molecules/cell in log phase SD medium.1 Publication

GO - Molecular functioni

  • unfolded protein binding Source: SGD

GO - Biological processi

  • sterol biosynthetic process Source: SGD

Enzyme and pathway databases

BioCyciYEAST:G3O-31171-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Protein NSG1
Alternative name(s):
INSIG homolog 1
Gene namesi
Name:NSG1
Ordered Locus Names:YHR133C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome VIII

Organism-specific databases

EuPathDBiFungiDB:YHR133C
SGDiS000001175 NSG1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 96CytoplasmicSequence analysisAdd BLAST96
Transmembranei97 – 117HelicalSequence analysisAdd BLAST21
Topological domaini118 – 156Perinuclear spaceSequence analysisAdd BLAST39
Transmembranei157 – 177HelicalSequence analysisAdd BLAST21
Topological domaini178 – 230CytoplasmicSequence analysisAdd BLAST53
Transmembranei231 – 251HelicalSequence analysisAdd BLAST21
Topological domaini252 – 261Perinuclear spaceSequence analysis10
Transmembranei262 – 282HelicalSequence analysisAdd BLAST21
Topological domaini283 – 291CytoplasmicSequence analysis9

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002029181 – 291Protein NSG1Add BLAST291

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei39PhosphoserineCombined sources1
Modified residuei76PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP38837
PaxDbiP38837
PRIDEiP38837

PTM databases

iPTMnetiP38837

Interactioni

GO - Molecular functioni

  • unfolded protein binding Source: SGD

Protein-protein interaction databases

BioGridi36566, 61 interactors
DIPiDIP-2775N
IntActiP38837, 9 interactors
MINTiP38837
STRINGi4932.YHR133C

Structurei

3D structure databases

ProteinModelPortaliP38837
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

To yeast YNL156c.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

GeneTreeiENSGT00530000066435
HOGENOMiHOG000113862
InParanoidiP38837
OrthoDBiEOG092C4AO9

Family and domain databases

InterProiView protein in InterPro
IPR025929 INSIG_fam
PANTHERiPTHR15301 PTHR15301, 1 hit
PfamiView protein in Pfam
PF07281 INSIG, 1 hit

Sequencei

Sequence statusi: Complete.

P38837-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGKKKSKNQL NTGGVPNGVH NTKKEAALPP LGNKLGSASF TAINTLTKPA
60 70 80 90 100
LFSFYDDDIT KNEGNVYDKA LLSNASQLEM VPPSATARHE RSLYAKIINT
110 120 130 140 150
IAAFFILFIA GILFPMISEC LFDNDQLAKG DIVSFLKHGI EIKNKIVAEP
160 170 180 190 200
DMVPDWAVFG TEGVIFGSIV PFIDSFVRYQ HQPKTRSSVY KNTLGSFIRC
210 220 230 240 250
ANTLLGLIFG IRKLEWSSSL QAAGAWSLLN IVLWLFFDGT LTVFFPGLVI
260 270 280 290
GALSAFTCSQ CFSQLSLALY FIDFYFFGFL MFSKLGRYLF N
Length:291
Mass (Da):32,214
Last modified:February 1, 1995 - v1
Checksum:iD0A634938AAD118E
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti8N → T in AAS56435 (PubMed:17322287).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U10398 Genomic DNA Translation: AAB68416.1
AY558109 Genomic DNA Translation: AAS56435.1
BK006934 Genomic DNA Translation: DAA06826.1
PIRiS48977
RefSeqiNP_012001.1, NM_001179263.1

Genome annotation databases

EnsemblFungiiYHR133C; YHR133C; YHR133C
GeneIDi856535
KEGGisce:YHR133C

Similar proteinsi

Entry informationi

Entry nameiNSG1_YEAST
AccessioniPrimary (citable) accession number: P38837
Secondary accession number(s): D3DL82, Q6Q5G1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: February 1, 1995
Last modified: May 23, 2018
This is version 122 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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