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P38837

- NSG1_YEAST

UniProt

P38837 - NSG1_YEAST

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Protein

Protein NSG1

Gene

NSG1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli

Functioni

Stabilizes the HMG-CoA reductase HMG2 by preventing its HRD1-dependent degradation. Binds directly to the sterol-sensing domain (SSD)-containing transmembrane region of HMG2, promoting its folding to protect it from degradation.1 Publication

GO - Molecular functioni

  1. unfolded protein binding Source: SGD

GO - Biological processi

  1. sterol biosynthetic process Source: SGD
Complete GO annotation...

Enzyme and pathway databases

BioCyciYEAST:G3O-31171-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Protein NSG1
Alternative name(s):
INSIG homolog 1
Gene namesi
Name:NSG1
Ordered Locus Names:YHR133C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome VIII

Organism-specific databases

CYGDiYHR133c.
SGDiS000001175. NSG1.

Subcellular locationi

Endoplasmic reticulum membrane 1 Publication; Multi-pass membrane protein 1 Publication

GO - Cellular componenti

  1. cytosol Source: SGD
  2. endoplasmic reticulum Source: SGD
  3. integral component of membrane Source: UniProtKB-KW
  4. nucleus Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 291291Protein NSG1PRO_0000202918Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei39 – 391Phosphoserine2 Publications
Modified residuei76 – 761Phosphoserine1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP38837.
PaxDbiP38837.

Expressioni

Gene expression databases

GenevestigatoriP38837.

Interactioni

Protein-protein interaction databases

BioGridi36566. 43 interactions.
DIPiDIP-2775N.
IntActiP38837. 9 interactions.
MINTiMINT-480589.
STRINGi4932.YHR133C.

Structurei

3D structure databases

ProteinModelPortaliP38837.
ModBaseiSearch...
MobiDBiSearch...

Topological domain

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 9696CytoplasmicSequence AnalysisAdd
BLAST
Topological domaini118 – 15639Perinuclear spaceSequence AnalysisAdd
BLAST
Topological domaini178 – 23053CytoplasmicSequence AnalysisAdd
BLAST
Topological domaini252 – 26110Perinuclear spaceSequence Analysis
Topological domaini283 – 2919CytoplasmicSequence Analysis

Transmembrane

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei97 – 11721HelicalSequence AnalysisAdd
BLAST
Transmembranei157 – 17721HelicalSequence AnalysisAdd
BLAST
Transmembranei231 – 25121HelicalSequence AnalysisAdd
BLAST
Transmembranei262 – 28221HelicalSequence AnalysisAdd
BLAST

Family & Domainsi

Sequence similaritiesi

To yeast YNL156c.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG39401.
GeneTreeiENSGT00530000066435.
HOGENOMiHOG000113862.
InParanoidiP38837.
OMAiFIRCANT.
OrthoDBiEOG7N0CH3.

Family and domain databases

InterProiIPR025929. INSIG_fam.
[Graphical view]
PfamiPF07281. INSIG. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P38837-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MGKKKSKNQL NTGGVPNGVH NTKKEAALPP LGNKLGSASF TAINTLTKPA
60 70 80 90 100
LFSFYDDDIT KNEGNVYDKA LLSNASQLEM VPPSATARHE RSLYAKIINT
110 120 130 140 150
IAAFFILFIA GILFPMISEC LFDNDQLAKG DIVSFLKHGI EIKNKIVAEP
160 170 180 190 200
DMVPDWAVFG TEGVIFGSIV PFIDSFVRYQ HQPKTRSSVY KNTLGSFIRC
210 220 230 240 250
ANTLLGLIFG IRKLEWSSSL QAAGAWSLLN IVLWLFFDGT LTVFFPGLVI
260 270 280 290
GALSAFTCSQ CFSQLSLALY FIDFYFFGFL MFSKLGRYLF N
Length:291
Mass (Da):32,214
Last modified:February 1, 1995 - v1
Checksum:iD0A634938AAD118E
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti8 – 81N → T in AAS56435. (PubMed:17322287)Curated

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U10398 Genomic DNA. Translation: AAB68416.1.
AY558109 Genomic DNA. Translation: AAS56435.1.
BK006934 Genomic DNA. Translation: DAA06826.1.
PIRiS48977.
RefSeqiNP_012001.1. NM_001179263.1.

Genome annotation databases

EnsemblFungiiYHR133C; YHR133C; YHR133C.
GeneIDi856535.
KEGGisce:YHR133C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U10398 Genomic DNA. Translation: AAB68416.1 .
AY558109 Genomic DNA. Translation: AAS56435.1 .
BK006934 Genomic DNA. Translation: DAA06826.1 .
PIRi S48977.
RefSeqi NP_012001.1. NM_001179263.1.

3D structure databases

ProteinModelPortali P38837.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 36566. 43 interactions.
DIPi DIP-2775N.
IntActi P38837. 9 interactions.
MINTi MINT-480589.
STRINGi 4932.YHR133C.

Proteomic databases

MaxQBi P38837.
PaxDbi P38837.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YHR133C ; YHR133C ; YHR133C .
GeneIDi 856535.
KEGGi sce:YHR133C.

Organism-specific databases

CYGDi YHR133c.
SGDi S000001175. NSG1.

Phylogenomic databases

eggNOGi NOG39401.
GeneTreei ENSGT00530000066435.
HOGENOMi HOG000113862.
InParanoidi P38837.
OMAi FIRCANT.
OrthoDBi EOG7N0CH3.

Enzyme and pathway databases

BioCyci YEAST:G3O-31171-MONOMER.

Miscellaneous databases

NextBioi 982316.

Gene expression databases

Genevestigatori P38837.

Family and domain databases

InterProi IPR025929. INSIG_fam.
[Graphical view ]
Pfami PF07281. INSIG. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  2. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  3. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  4. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  5. "INSIG: a broadly conserved transmembrane chaperone for sterol-sensing domain proteins."
    Flury I., Garza R., Shearer A., Rosen J., Cronin S., Hampton R.Y.
    EMBO J. 24:3917-3926(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION, INTERACTION WITH HMG2.
  6. "Quantitative phosphoproteomics applied to the yeast pheromone signaling pathway."
    Gruhler A., Olsen J.V., Mohammed S., Mortensen P., Faergeman N.J., Mann M., Jensen O.N.
    Mol. Cell. Proteomics 4:310-327(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-39, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Strain: YAL6B.
  7. "A global topology map of the Saccharomyces cerevisiae membrane proteome."
    Kim H., Melen K., Oesterberg M., von Heijne G.
    Proc. Natl. Acad. Sci. U.S.A. 103:11142-11147(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: TOPOLOGY [LARGE SCALE ANALYSIS].
    Strain: ATCC 208353 / W303-1A.
  8. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
    Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
    Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-76, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  9. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
    Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
    Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-39, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiNSG1_YEAST
AccessioniPrimary (citable) accession number: P38837
Secondary accession number(s): D3DL82, Q6Q5G1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: February 1, 1995
Last modified: October 29, 2014
This is version 99 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 1900 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  2. Yeast chromosome VIII
    Yeast (Saccharomyces cerevisiae) chromosome VIII: entries and gene names

External Data

Dasty 3