P38837 (NSG1_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Protein NSG1

Alternative name(s):
INSIG homolog 1

Gene names

Name:	NSG1
Ordered Locus Names:	YHR133C

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › Saccharomyces cerevisiae

Protein attributes

Sequence length	291 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Stabilizes the HMG-CoA reductase HMG2 by preventing its HRD1-dependent degradation. Binds directly to the sterol-sensing domain (SSD)-containing transmembrane region of HMG2, promoting its folding to protect it from degradation. Ref.5
Subcellular location	Endoplasmic reticulum membrane; Multi-pass membrane protein Ref.5.
Miscellaneous	Present with 1900 molecules/cell in log phase SD medium.
Sequence similarities	To yeast YNL156c.

Ontologies

Keywords
Cellular component	Endoplasmic reticulum Membrane
Domain	Transmembrane Transmembrane helix
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	sterol biosynthetic process Inferred from mutant phenotype Ref.5. Source: SGD
Cellular_component	endoplasmic reticulum Inferred from direct assay Ref.5. Source: SGD endoplasmic reticulum membrane Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Inferred from sequence model PubMed 12192589. Source: SGD nuclear periphery Inferred from direct assay PubMed 22842922. Source: SGD
Molecular_function	unfolded protein binding Inferred from mutant phenotype Ref.5. Source: SGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 291	291	Protein NSG1		PRO_0000202918
Regions
	Topological domain	1 – 96	96	Cytoplasmic Potential
	Transmembrane	97 – 117	21	Helical; Potential
	Topological domain	118 – 156	39	Perinuclear space Potential
	Transmembrane	157 – 177	21	Helical; Potential
	Topological domain	178 – 230	53	Cytoplasmic Potential
	Transmembrane	231 – 251	21	Helical; Potential
	Topological domain	252 – 261	10	Perinuclear space Potential
	Transmembrane	262 – 282	21	Helical; Potential
	Topological domain	283 – 291	9	Cytoplasmic Potential
Amino acid modifications
	Modified residue	39	1	Phosphoserine Ref.6 Ref.8
	Modified residue	76	1	Phosphoserine Ref.8
Experimental info
	Sequence conflict	8	1	N → T in AAS56435. Ref.3

Sequences

	Sequence		Length	Mass (Da)
	P38837 [UniParc]. Last modified February 1, 1995. Version 1. Checksum: D0A634938AAD118E	FASTA	291	32,214
	10 20 30 40 50 60 MGKKKSKNQL NTGGVPNGVH NTKKEAALPP LGNKLGSASF TAINTLTKPA LFSFYDDDIT 70 80 90 100 110 120 KNEGNVYDKA LLSNASQLEM VPPSATARHE RSLYAKIINT IAAFFILFIA GILFPMISEC 130 140 150 160 170 180 LFDNDQLAKG DIVSFLKHGI EIKNKIVAEP DMVPDWAVFG TEGVIFGSIV PFIDSFVRYQ 190 200 210 220 230 240 HQPKTRSSVY KNTLGSFIRC ANTLLGLIFG IRKLEWSSSL QAAGAWSLLN IVLWLFFDGT 250 260 270 280 290 LTVFFPGLVI GALSAFTCSQ CFSQLSLALY FIDFYFFGFL MFSKLGRYLF N

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Complete nucleotide sequence of Saccharomyces cerevisiae chromosome VIII." Johnston M., Andrews S., Brinkman R., Cooper J., Ding H., Dover J., Du Z., Favello A., Fulton L., Gattung S., Geisel C., Kirsten J., Kucaba T., Hillier L.W., Jier M., Johnston L., Langston Y., Latreille P. , Louis E.J., Macri C., Mardis E., Menezes S., Mouser L., Nhan M., Rifkin L., Riles L., St Peter H., Trevaskis E., Vaughan K., Vignati D., Wilcox L., Wohldman P., Waterston R., Wilson R., Vaudin M. Science 265:2077-2082(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[2]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[3]	"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae." Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J. Genome Res. 17:536-543(2007) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[4]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[5]	"INSIG: a broadly conserved transmembrane chaperone for sterol-sensing domain proteins." Flury I., Garza R., Shearer A., Rosen J., Cronin S., Hampton R.Y. EMBO J. 24:3917-3926(2005) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION, INTERACTION WITH HMG2.
[6]	"Quantitative phosphoproteomics applied to the yeast pheromone signaling pathway." Gruhler A., Olsen J.V., Mohammed S., Mortensen P., Faergeman N.J., Mann M., Jensen O.N. Mol. Cell. Proteomics 4:310-327(2005) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-39, MASS SPECTROMETRY. Strain: YAL6B.
[7]	"A global topology map of the Saccharomyces cerevisiae membrane proteome." Kim H., Melen K., Oesterberg M., von Heijne G. Proc. Natl. Acad. Sci. U.S.A. 103:11142-11147(2006) [PubMed] [Europe PMC] [Abstract] Cited for: TOPOLOGY [LARGE SCALE ANALYSIS]. Strain: ATCC 208353 / W303-1A.
[8]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-39 AND SER-76, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U10398 Genomic DNA. Translation: AAB68416.1. AY558109 Genomic DNA. Translation: AAS56435.1. BK006934 Genomic DNA. Translation: DAA06826.1.
PIR	S48977.
RefSeq	NP_012001.1. NM_001179263.1.
3D structure databases
ProteinModelPortal	P38837.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-2775N.
IntAct	P38837. 5 interactions.
MINT	MINT-480589.
STRING	4932.YHR133C.
Proteomic databases
PaxDb	P38837.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YHR133C; YHR133C; YHR133C.
GeneID	856535.
KEGG	sce:YHR133C.
Organism-specific databases
CYGD	YHR133c.
SGD	S000001175. NSG1.
Phylogenomic databases
eggNOG	NOG39401.
GeneTree	ENSGT00530000066435.
HOGENOM	HOG000113862.
OrthoDB	EOG42JS1Z.
Enzyme and pathway databases
BioCyc	YEAST:G3O-31171-MONOMER.
Gene expression databases
Genevestigator	P38837.
GermOnline	YHR133C. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR025929. INSIG_fam. [Graphical view]
Pfam	PF07281. INSIG. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	982316.

Entry information

Entry name

NSG1_YEAST

Accession

Primary (citable) accession number: P38837
Secondary accession number(s): D3DL82, Q6Q5G1

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1995
Last sequence update:	February 1, 1995
Last modified:	May 29, 2013
This is version 90 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program