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P38837 (NSG1_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 97. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Protein NSG1
Alternative name(s):
INSIG homolog 1
Gene names
Name:NSG1
Ordered Locus Names:YHR133C
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length291 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Stabilizes the HMG-CoA reductase HMG2 by preventing its HRD1-dependent degradation. Binds directly to the sterol-sensing domain (SSD)-containing transmembrane region of HMG2, promoting its folding to protect it from degradation. Ref.5

Subcellular location

Endoplasmic reticulum membrane; Multi-pass membrane protein Ref.5.

Miscellaneous

Present with 1900 molecules/cell in log phase SD medium.

Sequence similarities

To yeast YNL156c.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 291291Protein NSG1
PRO_0000202918

Regions

Topological domain1 – 9696Cytoplasmic Potential
Transmembrane97 – 11721Helical; Potential
Topological domain118 – 15639Perinuclear space Potential
Transmembrane157 – 17721Helical; Potential
Topological domain178 – 23053Cytoplasmic Potential
Transmembrane231 – 25121Helical; Potential
Topological domain252 – 26110Perinuclear space Potential
Transmembrane262 – 28221Helical; Potential
Topological domain283 – 2919Cytoplasmic Potential

Amino acid modifications

Modified residue391Phosphoserine Ref.6 Ref.9
Modified residue761Phosphoserine Ref.8

Experimental info

Sequence conflict81N → T in AAS56435. Ref.3

Sequences

Sequence LengthMass (Da)Tools
P38837 [UniParc].

Last modified February 1, 1995. Version 1.
Checksum: D0A634938AAD118E

FASTA29132,214
        10         20         30         40         50         60 
MGKKKSKNQL NTGGVPNGVH NTKKEAALPP LGNKLGSASF TAINTLTKPA LFSFYDDDIT 

        70         80         90        100        110        120 
KNEGNVYDKA LLSNASQLEM VPPSATARHE RSLYAKIINT IAAFFILFIA GILFPMISEC 

       130        140        150        160        170        180 
LFDNDQLAKG DIVSFLKHGI EIKNKIVAEP DMVPDWAVFG TEGVIFGSIV PFIDSFVRYQ 

       190        200        210        220        230        240 
HQPKTRSSVY KNTLGSFIRC ANTLLGLIFG IRKLEWSSSL QAAGAWSLLN IVLWLFFDGT 

       250        260        270        280        290 
LTVFFPGLVI GALSAFTCSQ CFSQLSLALY FIDFYFFGFL MFSKLGRYLF N 

« Hide

References

« Hide 'large scale' references
[1]"Complete nucleotide sequence of Saccharomyces cerevisiae chromosome VIII."
Johnston M., Andrews S., Brinkman R., Cooper J., Ding H., Dover J., Du Z., Favello A., Fulton L., Gattung S., Geisel C., Kirsten J., Kucaba T., Hillier L.W., Jier M., Johnston L., Langston Y., Latreille P. expand/collapse author list , Louis E.J., Macri C., Mardis E., Menezes S., Mouser L., Nhan M., Rifkin L., Riles L., St Peter H., Trevaskis E., Vaughan K., Vignati D., Wilcox L., Wohldman P., Waterston R., Wilson R., Vaudin M.
Science 265:2077-2082(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[2]"The reference genome sequence of Saccharomyces cerevisiae: Then and now."
Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R., Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S., Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.
G3 (Bethesda) 4:389-398(2014) [PubMed] [Europe PMC] [Abstract]
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[3]"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae."
Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. expand/collapse author list , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J.
Genome Res. 17:536-543(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[4]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[5]"INSIG: a broadly conserved transmembrane chaperone for sterol-sensing domain proteins."
Flury I., Garza R., Shearer A., Rosen J., Cronin S., Hampton R.Y.
EMBO J. 24:3917-3926(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, SUBCELLULAR LOCATION, INTERACTION WITH HMG2.
[6]"Quantitative phosphoproteomics applied to the yeast pheromone signaling pathway."
Gruhler A., Olsen J.V., Mohammed S., Mortensen P., Faergeman N.J., Mann M., Jensen O.N.
Mol. Cell. Proteomics 4:310-327(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-39, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Strain: YAL6B.
[7]"A global topology map of the Saccharomyces cerevisiae membrane proteome."
Kim H., Melen K., Oesterberg M., von Heijne G.
Proc. Natl. Acad. Sci. U.S.A. 103:11142-11147(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: TOPOLOGY [LARGE SCALE ANALYSIS].
Strain: ATCC 208353 / W303-1A.
[8]"A multidimensional chromatography technology for in-depth phosphoproteome analysis."
Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-76, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[9]"Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-39, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U10398 Genomic DNA. Translation: AAB68416.1.
AY558109 Genomic DNA. Translation: AAS56435.1.
BK006934 Genomic DNA. Translation: DAA06826.1.
PIRS48977.
RefSeqNP_012001.1. NM_001179263.1.

3D structure databases

ProteinModelPortalP38837.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid36566. 43 interactions.
DIPDIP-2775N.
IntActP38837. 9 interactions.
MINTMINT-480589.
STRING4932.YHR133C.

Proteomic databases

MaxQBP38837.
PaxDbP38837.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYHR133C; YHR133C; YHR133C.
GeneID856535.
KEGGsce:YHR133C.

Organism-specific databases

CYGDYHR133c.
SGDS000001175. NSG1.

Phylogenomic databases

eggNOGNOG39401.
GeneTreeENSGT00530000066435.
HOGENOMHOG000113862.
OMAFIRCANT.
OrthoDBEOG7N0CH3.

Enzyme and pathway databases

BioCycYEAST:G3O-31171-MONOMER.

Gene expression databases

GenevestigatorP38837.

Family and domain databases

InterProIPR025929. INSIG_fam.
[Graphical view]
PfamPF07281. INSIG. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio982316.

Entry information

Entry nameNSG1_YEAST
AccessionPrimary (citable) accession number: P38837
Secondary accession number(s): D3DL82, Q6Q5G1
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: February 1, 1995
Last modified: May 14, 2014
This is version 97 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome VIII

Yeast (Saccharomyces cerevisiae) chromosome VIII: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD