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P38837

- NSG1_YEAST

UniProt

P38837 - NSG1_YEAST

Protein

Protein NSG1

Gene

NSG1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 98 (01 Oct 2014)
      Sequence version 1 (01 Feb 1995)
      Previous versions | rss
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    Functioni

    Stabilizes the HMG-CoA reductase HMG2 by preventing its HRD1-dependent degradation. Binds directly to the sterol-sensing domain (SSD)-containing transmembrane region of HMG2, promoting its folding to protect it from degradation.1 Publication

    GO - Molecular functioni

    1. unfolded protein binding Source: SGD

    GO - Biological processi

    1. sterol biosynthetic process Source: SGD

    Enzyme and pathway databases

    BioCyciYEAST:G3O-31171-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Protein NSG1
    Alternative name(s):
    INSIG homolog 1
    Gene namesi
    Name:NSG1
    Ordered Locus Names:YHR133C
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome VIII

    Organism-specific databases

    CYGDiYHR133c.
    SGDiS000001175. NSG1.

    Subcellular locationi

    Endoplasmic reticulum membrane 1 Publication; Multi-pass membrane protein 1 Publication

    GO - Cellular componenti

    1. cytosol Source: SGD
    2. endoplasmic reticulum Source: SGD
    3. endoplasmic reticulum membrane Source: UniProtKB-SubCell
    4. integral component of membrane Source: UniProtKB-KW
    5. nucleus Source: SGD

    Keywords - Cellular componenti

    Endoplasmic reticulum, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 291291Protein NSG1PRO_0000202918Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei39 – 391Phosphoserine2 Publications
    Modified residuei76 – 761Phosphoserine1 Publication

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    MaxQBiP38837.
    PaxDbiP38837.

    Expressioni

    Gene expression databases

    GenevestigatoriP38837.

    Interactioni

    Protein-protein interaction databases

    BioGridi36566. 43 interactions.
    DIPiDIP-2775N.
    IntActiP38837. 9 interactions.
    MINTiMINT-480589.
    STRINGi4932.YHR133C.

    Structurei

    3D structure databases

    ProteinModelPortaliP38837.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini1 – 9696CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini118 – 15639Perinuclear spaceSequence AnalysisAdd
    BLAST
    Topological domaini178 – 23053CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini252 – 26110Perinuclear spaceSequence Analysis
    Topological domaini283 – 2919CytoplasmicSequence Analysis

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei97 – 11721HelicalSequence AnalysisAdd
    BLAST
    Transmembranei157 – 17721HelicalSequence AnalysisAdd
    BLAST
    Transmembranei231 – 25121HelicalSequence AnalysisAdd
    BLAST
    Transmembranei262 – 28221HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Sequence similaritiesi

    To yeast YNL156c.Curated

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiNOG39401.
    GeneTreeiENSGT00530000066435.
    HOGENOMiHOG000113862.
    OMAiFIRCANT.
    OrthoDBiEOG7N0CH3.

    Family and domain databases

    InterProiIPR025929. INSIG_fam.
    [Graphical view]
    PfamiPF07281. INSIG. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P38837-1 [UniParc]FASTAAdd to Basket

    « Hide

    MGKKKSKNQL NTGGVPNGVH NTKKEAALPP LGNKLGSASF TAINTLTKPA    50
    LFSFYDDDIT KNEGNVYDKA LLSNASQLEM VPPSATARHE RSLYAKIINT 100
    IAAFFILFIA GILFPMISEC LFDNDQLAKG DIVSFLKHGI EIKNKIVAEP 150
    DMVPDWAVFG TEGVIFGSIV PFIDSFVRYQ HQPKTRSSVY KNTLGSFIRC 200
    ANTLLGLIFG IRKLEWSSSL QAAGAWSLLN IVLWLFFDGT LTVFFPGLVI 250
    GALSAFTCSQ CFSQLSLALY FIDFYFFGFL MFSKLGRYLF N 291
    Length:291
    Mass (Da):32,214
    Last modified:February 1, 1995 - v1
    Checksum:iD0A634938AAD118E
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti8 – 81N → T in AAS56435. (PubMed:17322287)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U10398 Genomic DNA. Translation: AAB68416.1.
    AY558109 Genomic DNA. Translation: AAS56435.1.
    BK006934 Genomic DNA. Translation: DAA06826.1.
    PIRiS48977.
    RefSeqiNP_012001.1. NM_001179263.1.

    Genome annotation databases

    EnsemblFungiiYHR133C; YHR133C; YHR133C.
    GeneIDi856535.
    KEGGisce:YHR133C.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U10398 Genomic DNA. Translation: AAB68416.1 .
    AY558109 Genomic DNA. Translation: AAS56435.1 .
    BK006934 Genomic DNA. Translation: DAA06826.1 .
    PIRi S48977.
    RefSeqi NP_012001.1. NM_001179263.1.

    3D structure databases

    ProteinModelPortali P38837.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 36566. 43 interactions.
    DIPi DIP-2775N.
    IntActi P38837. 9 interactions.
    MINTi MINT-480589.
    STRINGi 4932.YHR133C.

    Proteomic databases

    MaxQBi P38837.
    PaxDbi P38837.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YHR133C ; YHR133C ; YHR133C .
    GeneIDi 856535.
    KEGGi sce:YHR133C.

    Organism-specific databases

    CYGDi YHR133c.
    SGDi S000001175. NSG1.

    Phylogenomic databases

    eggNOGi NOG39401.
    GeneTreei ENSGT00530000066435.
    HOGENOMi HOG000113862.
    OMAi FIRCANT.
    OrthoDBi EOG7N0CH3.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-31171-MONOMER.

    Miscellaneous databases

    NextBioi 982316.

    Gene expression databases

    Genevestigatori P38837.

    Family and domain databases

    InterProi IPR025929. INSIG_fam.
    [Graphical view ]
    Pfami PF07281. INSIG. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    2. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    3. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    4. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
    5. "INSIG: a broadly conserved transmembrane chaperone for sterol-sensing domain proteins."
      Flury I., Garza R., Shearer A., Rosen J., Cronin S., Hampton R.Y.
      EMBO J. 24:3917-3926(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, SUBCELLULAR LOCATION, INTERACTION WITH HMG2.
    6. "Quantitative phosphoproteomics applied to the yeast pheromone signaling pathway."
      Gruhler A., Olsen J.V., Mohammed S., Mortensen P., Faergeman N.J., Mann M., Jensen O.N.
      Mol. Cell. Proteomics 4:310-327(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-39, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Strain: YAL6B.
    7. "A global topology map of the Saccharomyces cerevisiae membrane proteome."
      Kim H., Melen K., Oesterberg M., von Heijne G.
      Proc. Natl. Acad. Sci. U.S.A. 103:11142-11147(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: TOPOLOGY [LARGE SCALE ANALYSIS].
      Strain: ATCC 208353 / W303-1A.
    8. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
      Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
      Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-76, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    9. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
      Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
      Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-39, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiNSG1_YEAST
    AccessioniPrimary (citable) accession number: P38837
    Secondary accession number(s): D3DL82, Q6Q5G1
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 1, 1995
    Last sequence update: February 1, 1995
    Last modified: October 1, 2014
    This is version 98 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 1900 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    2. Yeast chromosome VIII
      Yeast (Saccharomyces cerevisiae) chromosome VIII: entries and gene names

    External Data

    Dasty 3