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Protein

Putative metallocarboxypeptidase ECM14

Gene

ECM14

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Probable carboxypeptidase that may be involved in cell wall organization and biogenesis.

Cofactori

Zn2+By similarityNote: Binds 1 zinc ion per subunit.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi182 – 1821Zinc; catalyticBy similarity
Metal bindingi185 – 1851Zinc; catalyticBy similarity
Binding sitei240 – 2401SubstrateBy similarity
Metal bindingi310 – 3101Zinc; catalyticBy similarity

GO - Molecular functioni

  • metallocarboxypeptidase activity Source: InterPro
  • metalloendopeptidase activity Source: SGD
  • zinc ion binding Source: InterPro

GO - Biological processi

  • cell wall organization Source: UniProtKB-KW
  • proteolysis Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Carboxypeptidase, Hydrolase, Metalloprotease, Protease

Keywords - Biological processi

Cell wall biogenesis/degradation

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

BioCyciYEAST:G3O-31170-MONOMER.

Protein family/group databases

MEROPSiM14.014.

Names & Taxonomyi

Protein namesi
Recommended name:
Putative metallocarboxypeptidase ECM14 (EC:3.4.17.-)
Alternative name(s):
Extracellular mutant protein 14
Gene namesi
Name:ECM14
Ordered Locus Names:YHR132C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome VIII

Organism-specific databases

EuPathDBiFungiDB:YHR132C.
SGDiS000001174. ECM14.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Vacuole

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2424Sequence analysisAdd
BLAST
Chaini25 – 430406Putative metallocarboxypeptidase ECM14PRO_0000004417Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi41 – 411N-linked (GlcNAc...)Sequence analysis
Disulfide bondi251 ↔ 272By similarity
Glycosylationi295 – 2951N-linked (GlcNAc...)Sequence analysis

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

MaxQBiP38836.
PeptideAtlasiP38836.

Interactioni

Protein-protein interaction databases

BioGridi36564. 37 interactions.
DIPiDIP-4615N.
IntActiP38836. 2 interactions.
MINTiMINT-492279.

Structurei

3D structure databases

ProteinModelPortaliP38836.
SMRiP38836. Positions 84-427.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni182 – 1854Substrate bindingBy similarity
Regioni257 – 2582Substrate bindingBy similarity
Regioni311 – 3122Substrate bindingBy similarity

Sequence similaritiesi

Belongs to the peptidase M14 family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

GeneTreeiENSGT00760000119103.
HOGENOMiHOG000214206.
InParanoidiP38836.
KOiK08783.
OMAiCGEEYSG.
OrthoDBiEOG7Z0K5M.

Family and domain databases

InterProiIPR000834. Peptidase_M14.
[Graphical view]
PfamiPF00246. Peptidase_M14. 1 hit.
[Graphical view]
PRINTSiPR00765. CRBOXYPTASEA.
SMARTiSM00631. Zn_pept. 1 hit.
[Graphical view]
PROSITEiPS00132. CARBOXYPEPT_ZN_1. 1 hit.
PS00133. CARBOXYPEPT_ZN_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P38836-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLHMNSLWGC FLFVLLAVTG AVQGLQEDYS EYAVYRFTSD NYSTLVRDVI
60 70 80 90 100
APLTDDYDVW TRSNNFIDIK LPKEIGEQIN DGQVIIDNMN ELIQNTLPTS
110 120 130 140 150
QMMAREQAVF ENDYDFFFNE YRDLDTIYMW LDLLERSFPS LVAVEHLGRT
160 170 180 190 200
FEGRELKALH ISGNKPESNP EKKTIVITGG IHAREWISVS TVCWALYQLL
210 220 230 240 250
NRYGSSKKET KYLDDLDFLV IPVFNPDGYA YTWSHDRLWR KNRQRTHVPQ
260 270 280 290 300
CLGIDIDHSF GFQWEKAHTH ACSEEYSGET PFEAWEASAW YKYINETKGD
310 320 330 340 350
YKIYGYIDMH SYSQEILYPY AYSCDALPRD LENLLELSYG LSKAIRSKSG
360 370 380 390 400
RNYDVISACK DRGSDIFPGL GAGSALDFMY HHRAHWAFQL KLRDTGNHGF
410 420 430
LLPPENIKPV GKETYAALKY FCDFLLDPEI
Length:430
Mass (Da):49,829
Last modified:February 1, 1995 - v1
Checksum:i1B9F5C0D18266A88
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U10398 Genomic DNA. Translation: AAB68415.1.
BK006934 Genomic DNA. Translation: DAA06824.1.
PIRiS48976.
RefSeqiNP_012000.1. NM_001179262.1.

Genome annotation databases

EnsemblFungiiYHR132C; YHR132C; YHR132C.
GeneIDi856533.
KEGGisce:YHR132C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U10398 Genomic DNA. Translation: AAB68415.1.
BK006934 Genomic DNA. Translation: DAA06824.1.
PIRiS48976.
RefSeqiNP_012000.1. NM_001179262.1.

3D structure databases

ProteinModelPortaliP38836.
SMRiP38836. Positions 84-427.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi36564. 37 interactions.
DIPiDIP-4615N.
IntActiP38836. 2 interactions.
MINTiMINT-492279.

Protein family/group databases

MEROPSiM14.014.

Proteomic databases

MaxQBiP38836.
PeptideAtlasiP38836.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYHR132C; YHR132C; YHR132C.
GeneIDi856533.
KEGGisce:YHR132C.

Organism-specific databases

EuPathDBiFungiDB:YHR132C.
SGDiS000001174. ECM14.

Phylogenomic databases

GeneTreeiENSGT00760000119103.
HOGENOMiHOG000214206.
InParanoidiP38836.
KOiK08783.
OMAiCGEEYSG.
OrthoDBiEOG7Z0K5M.

Enzyme and pathway databases

BioCyciYEAST:G3O-31170-MONOMER.

Miscellaneous databases

NextBioi982310.
PROiP38836.

Family and domain databases

InterProiIPR000834. Peptidase_M14.
[Graphical view]
PfamiPF00246. Peptidase_M14. 1 hit.
[Graphical view]
PRINTSiPR00765. CRBOXYPTASEA.
SMARTiSM00631. Zn_pept. 1 hit.
[Graphical view]
PROSITEiPS00132. CARBOXYPEPT_ZN_1. 1 hit.
PS00133. CARBOXYPEPT_ZN_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  2. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  3. "Large scale identification of genes involved in cell surface biosynthesis and architecture in Saccharomyces cerevisiae."
    Lussier M., White A.-M., Sheraton J., di Paolo T., Treadwell J., Southard S.B., Horenstein C.I., Chen-Weiner J., Ram A.F.J., Kapteyn J.C., Roemer T.W., Vo D.H., Bondoc D.C., Hall J., Zhong W.-W., Sdicu A.-M., Davies J., Klis F.M., Robbins P.W., Bussey H.
    Genetics 147:435-450(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION.
  4. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
  5. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiECM14_YEAST
AccessioniPrimary (citable) accession number: P38836
Secondary accession number(s): D3DL80
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: February 1, 1995
Last modified: May 11, 2016
This is version 137 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 468 molecules/cell in log phase SD medium.1 Publication

Caution

Lacks the conserved Glu residue in position 391 essential for carbopeptidase activity. Its enzyme activity is therefore unsure.Curated

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome VIII
    Yeast (Saccharomyces cerevisiae) chromosome VIII: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.