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Protein

Putative metallocarboxypeptidase ECM14

Gene

ECM14

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Probable carboxypeptidase that may be involved in cell wall organization and biogenesis.

Miscellaneous

Present with 468 molecules/cell in log phase SD medium.1 Publication

Caution

Lacks the conserved Glu residue in position 391 essential for carbopeptidase activity. Its enzyme activity is therefore unsure.Curated

Cofactori

Zn2+By similarityNote: Binds 1 zinc ion per subunit.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi182Zinc; catalyticBy similarity1
Metal bindingi185Zinc; catalyticBy similarity1
Binding sitei240SubstrateBy similarity1
Metal bindingi310Zinc; catalyticBy similarity1

GO - Molecular functioni

  • metallocarboxypeptidase activity Source: GO_Central
  • metalloendopeptidase activity Source: SGD
  • zinc ion binding Source: InterPro

GO - Biological processi

  • cell wall organization Source: UniProtKB-KW
  • proteolysis Source: SGD

Keywordsi

Molecular functionCarboxypeptidase, Hydrolase, Metalloprotease, Protease
Biological processCell wall biogenesis/degradation
LigandMetal-binding, Zinc

Enzyme and pathway databases

BioCyciYEAST:G3O-31170-MONOMER

Protein family/group databases

MEROPSiM14.014

Names & Taxonomyi

Protein namesi
Recommended name:
Putative metallocarboxypeptidase ECM14 (EC:3.4.17.-)
Alternative name(s):
Extracellular mutant protein 14
Gene namesi
Name:ECM14
Ordered Locus Names:YHR132C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome VIII

Organism-specific databases

EuPathDBiFungiDB:YHR132C
SGDiS000001174 ECM14

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Vacuole

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 24Sequence analysisAdd BLAST24
ChainiPRO_000000441725 – 430Putative metallocarboxypeptidase ECM14Add BLAST406

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi41N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi251 ↔ 272By similarity
Glycosylationi295N-linked (GlcNAc...) asparagineSequence analysis1

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

MaxQBiP38836
PaxDbiP38836
PRIDEiP38836

Interactioni

Protein-protein interaction databases

BioGridi36564, 69 interactors
DIPiDIP-4615N
IntActiP38836, 2 interactors
STRINGi4932.YHR132C

Structurei

3D structure databases

ProteinModelPortaliP38836
SMRiP38836
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni182 – 185Substrate bindingBy similarity4
Regioni257 – 258Substrate bindingBy similarity2
Regioni311 – 312Substrate bindingBy similarity2

Sequence similaritiesi

Belongs to the peptidase M14 family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

GeneTreeiENSGT00760000119103
HOGENOMiHOG000214206
InParanoidiP38836
KOiK08783
OMAiRKHIRAY
OrthoDBiEOG092C238F

Family and domain databases

InterProiView protein in InterPro
IPR000834 Peptidase_M14
PfamiView protein in Pfam
PF00246 Peptidase_M14, 1 hit
PRINTSiPR00765 CRBOXYPTASEA
SMARTiView protein in SMART
SM00631 Zn_pept, 1 hit
PROSITEiView protein in PROSITE
PS00132 CARBOXYPEPT_ZN_1, 1 hit
PS00133 CARBOXYPEPT_ZN_2, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P38836-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLHMNSLWGC FLFVLLAVTG AVQGLQEDYS EYAVYRFTSD NYSTLVRDVI
60 70 80 90 100
APLTDDYDVW TRSNNFIDIK LPKEIGEQIN DGQVIIDNMN ELIQNTLPTS
110 120 130 140 150
QMMAREQAVF ENDYDFFFNE YRDLDTIYMW LDLLERSFPS LVAVEHLGRT
160 170 180 190 200
FEGRELKALH ISGNKPESNP EKKTIVITGG IHAREWISVS TVCWALYQLL
210 220 230 240 250
NRYGSSKKET KYLDDLDFLV IPVFNPDGYA YTWSHDRLWR KNRQRTHVPQ
260 270 280 290 300
CLGIDIDHSF GFQWEKAHTH ACSEEYSGET PFEAWEASAW YKYINETKGD
310 320 330 340 350
YKIYGYIDMH SYSQEILYPY AYSCDALPRD LENLLELSYG LSKAIRSKSG
360 370 380 390 400
RNYDVISACK DRGSDIFPGL GAGSALDFMY HHRAHWAFQL KLRDTGNHGF
410 420 430
LLPPENIKPV GKETYAALKY FCDFLLDPEI
Length:430
Mass (Da):49,829
Last modified:February 1, 1995 - v1
Checksum:i1B9F5C0D18266A88
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U10398 Genomic DNA Translation: AAB68415.1
BK006934 Genomic DNA Translation: DAA06824.1
PIRiS48976
RefSeqiNP_012000.1, NM_001179262.1

Genome annotation databases

EnsemblFungiiYHR132C; YHR132C; YHR132C
GeneIDi856533
KEGGisce:YHR132C

Similar proteinsi

Entry informationi

Entry nameiECM14_YEAST
AccessioniPrimary (citable) accession number: P38836
Secondary accession number(s): D3DL80
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: February 1, 1995
Last modified: May 23, 2018
This is version 154 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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