ID   ANS1_YEAST              Reviewed;         159 AA.
AC   P38832; D3DL75;
DT   01-FEB-1995, integrated into UniProtKB/Swiss-Prot.
DT   01-FEB-1995, sequence version 1.
DT   08-NOV-2023, entry version 116.
DE   RecName: Full=Probable GPI-anchored protein ANS1;
DE   Flags: Precursor;
GN   Name=ANS1; OrderedLocusNames=YHR126C;
OS   Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC   Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX   NCBI_TaxID=559292;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 204508 / S288c;
RX   PubMed=8091229; DOI=10.1126/science.8091229;
RA   Johnston M., Andrews S., Brinkman R., Cooper J., Ding H., Dover J., Du Z.,
RA   Favello A., Fulton L., Gattung S., Geisel C., Kirsten J., Kucaba T.,
RA   Hillier L.W., Jier M., Johnston L., Langston Y., Latreille P., Louis E.J.,
RA   Macri C., Mardis E., Menezes S., Mouser L., Nhan M., Rifkin L., Riles L.,
RA   St Peter H., Trevaskis E., Vaughan K., Vignati D., Wilcox L., Wohldman P.,
RA   Waterston R., Wilson R., Vaudin M.;
RT   "Complete nucleotide sequence of Saccharomyces cerevisiae chromosome
RT   VIII.";
RL   Science 265:2077-2082(1994).
RN   [2]
RP   GENOME REANNOTATION.
RC   STRAIN=ATCC 204508 / S288c;
RX   PubMed=24374639; DOI=10.1534/g3.113.008995;
RA   Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA   Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA   Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT   "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL   G3 (Bethesda) 4:389-398(2014).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=ATCC 204508 / S288c;
RX   PubMed=17322287; DOI=10.1101/gr.6037607;
RA   Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F.,
RA   Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J.,
RA   Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J.,
RA   Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D.,
RA   LaBaer J.;
RT   "Approaching a complete repository of sequence-verified protein-encoding
RT   clones for Saccharomyces cerevisiae.";
RL   Genome Res. 17:536-543(2007).
RN   [4]
RP   INDUCTION.
RX   PubMed=16467472; DOI=10.1128/ec.5.2.313-320.2006;
RA   Slattery M.G., Liko D., Heideman W.;
RT   "The function and properties of the Azf1 transcriptional regulator change
RT   with growth conditions in Saccharomyces cerevisiae.";
RL   Eukaryot. Cell 5:313-320(2006).
CC   -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000305}; Lipid-anchor, GPI-
CC       anchor {ECO:0000305}.
CC   -!- INDUCTION: Expression is regulated by AZF1.
CC       {ECO:0000269|PubMed:16467472}.
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DR   EMBL; U10398; AAB68411.1; -; Genomic_DNA.
DR   EMBL; AY692674; AAT92693.1; -; Genomic_DNA.
DR   EMBL; BK006934; DAA06819.1; -; Genomic_DNA.
DR   PIR; S48970; S48970.
DR   RefSeq; NP_011994.1; NM_001179256.1.
DR   AlphaFoldDB; P38832; -.
DR   BioGRID; 36559; 15.
DR   STRING; 4932.YHR126C; -.
DR   PaxDb; 4932-YHR126C; -.
DR   EnsemblFungi; YHR126C_mRNA; YHR126C; YHR126C.
DR   GeneID; 856527; -.
DR   KEGG; sce:YHR126C; -.
DR   AGR; SGD:S000001168; -.
DR   SGD; S000001168; ANS1.
DR   VEuPathDB; FungiDB:YHR126C; -.
DR   HOGENOM; CLU_1678931_0_0_1; -.
DR   InParanoid; P38832; -.
DR   OrthoDB; 2028776at2759; -.
DR   BioCyc; YEAST:G3O-31166-MONOMER; -.
DR   BioGRID-ORCS; 856527; 7 hits in 10 CRISPR screens.
DR   PRO; PR:P38832; -.
DR   Proteomes; UP000002311; Chromosome VIII.
DR   RNAct; P38832; Protein.
DR   GO; GO:0000324; C:fungal-type vacuole; HDA:SGD.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0098552; C:side of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005199; F:structural constituent of cell wall; IEA:InterPro.
DR   InterPro; IPR000420; Yeast_PIR.
DR   Pfam; PF00399; PIR; 1.
DR   PROSITE; PS00929; PIR_REPEAT_1; 1.
DR   PROSITE; PS50256; PIR_REPEAT_2; 1.
PE   2: Evidence at transcript level;
KW   Cell membrane; Glycoprotein; GPI-anchor; Lipoprotein; Membrane;
KW   Reference proteome; Signal.
FT   SIGNAL          1..20
FT                   /evidence="ECO:0000255"
FT   CHAIN           21..137
FT                   /note="Probable GPI-anchored protein ANS1"
FT                   /id="PRO_0000203777"
FT   PROPEP          138..159
FT                   /note="Removed in mature form"
FT                   /evidence="ECO:0000255"
FT                   /id="PRO_0000402190"
FT   REPEAT          101..114
FT                   /note="PIR1/2/3"
FT   LIPID           137
FT                   /note="GPI-anchor amidated glycine"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   159 AA;  16635 MW;  EFF6F5F5A6DEA211 CRC64;
     MKCTLVSTLF AITNILVAHA QVSNSSDTLD VQFANSTNSY IEGKFNSTDE AFNSSASWSL
     AAQQKKISNA AVYDVGGWNG SLYRSNRSAV ADHQPGKKQD AAISQISDGQ IQATASGPET
     TAATTPSSTA NVSVYEGAGM KVESKNMGYI VGVAALLFL
//