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P38830 (NDT80_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 114. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Meiosis-specific transcription factor NDT80
Gene names
Name:NDT80
Synonyms:DAS1
Ordered Locus Names:YHR124W
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length627 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Transcription factor required for successful completion of meiosis and spore formation. Gets activated after completion of meiotic recombination at the end of prophase I. Recognizes and binds to the middle sporulation element (MSE) 5'-C[AG]CAAA[AT]-3' in the promoter region of stage-specific genes that are required for progression through meiosis and sporulation. Competes for binding to MSE with the transcriptional repressor SUM1, which represses middle sporulation-specific genes during mitosis and early sporulation. Ref.1 Ref.5 Ref.6 Ref.11

Subunit structure

Binds to DNA as a monomer. Ref.16

Subcellular location

Nucleus Ref.7.

Induction

Induced during prophase I of meiosis. Requires protein kinase IME2 for initial expression and continued transcription during meiotic divisions. Can autoactivate its own synthesis. Ref.6 Ref.8 Ref.10 Ref.12

Post-translational modification

Phosphorylated by pachytene checkpoint kinase IME2, but also phosphorylated in an IME2-independent manner. Phosphorylation probably eliminates SUM1-mediated repression and is also required for full transcriptional activation activity. Phosphorylation of the DNA-binding domain by IME2 does not alter DNA binding affinity. Ref.7 Ref.9 Ref.10 Ref.12 Ref.14

Sequence similarities

Contains 1 NDT80 DNA-binding domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 627627Meiosis-specific transcription factor NDT80
PRO_0000096772

Regions

DNA binding28 – 335308NDT80 Ref.5 Ref.13 Ref.14

Sites

Site581Interaction with DNA
Site1111Interaction with DNA
Site1771Interaction with DNA
Site2081Interaction with DNA
Site2541Interaction with DNA
Site3261Interaction with DNA

Experimental info

Mutagenesis501K → A: Reduces DNA-binding by 70%. Ref.13
Mutagenesis541K → A: Reduces DNA-binding by 50%. Ref.13
Mutagenesis571P → A: Reduces DNA-binding by 65%.
Mutagenesis581R → A: Reduces DNA-binding by 65%.
Mutagenesis591S → A: Reduces DNA-binding by 86%.
Mutagenesis971R → A: Reduces DNA-binding by 67%. Ref.13
Mutagenesis1101K → A: No effect on DNA-binding but strongly reduces progress through meiosis and sporulation. Ref.16
Mutagenesis1111R → A: Reduces DNA-binding by 95% and abolishes sporulation. Ref.13 Ref.16
Mutagenesis1131Y → A: Reduces DNA-binding by 80% and abolishes sporulation. Ref.13 Ref.16
Mutagenesis1731H → A: Reduces DNA-binding by 80% and strongly reduces progress through meiosis and sporulation. Ref.16
Mutagenesis1761K → A: Reduces DNA-binding by 50% but does not abolish sporulation. Ref.16
Mutagenesis1771R → A: Reduces DNA-binding by 96% and abolishes sporulation. Ref.16
Mutagenesis2021R → A: No effect on DNA-binding but strongly reduces progress through meiosis and sporulation. Ref.16
Mutagenesis2081R → A: Reduces DNA-binding by 50% and abolishes sporulation. Ref.13 Ref.16
Mutagenesis2541R → A: Reduces DNA-binding by 93% and abolishes sporulation. Ref.13 Ref.16
Mutagenesis3261R → A: Reduces DNA-binding by 50% and abolishes sporulation. Ref.13 Ref.16
Sequence conflict2001I → T Ref.15
Sequence conflict2131M → T Ref.16
Sequence conflict2251D → N Ref.16
Sequence conflict2671S → F Ref.16
Sequence conflict3631L → S in AAU09742. Ref.4
Sequence conflict4931F → L in AAA92299. Ref.1
Sequence conflict5681P → N in AAA92299. Ref.1

Secondary structure

.................................................................... 627
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P38830 [UniParc].

Last modified June 15, 2010. Version 4.
Checksum: 947DA0BA08065595

FASTA62771,479
        10         20         30         40         50         60 
MNEMENTDPV LQDDLVSKYE RELSTEQEED TPVILTQLNE DGTTSNYFDK RKLKIAPRST 

        70         80         90        100        110        120 
LQFKVGPPFE LVRDYCPVVE SHTGRTLDLR IIPRIDRGFD HIDEEWVGYK RNYFTLVSTF 

       130        140        150        160        170        180 
ETANCDLDTF LKSSFDLLVE DSSVESRLRV QYFAIKIKAK NDDDDTEINL VQHTAKRDKG 

       190        200        210        220        230        240 
PQFCPSVCPL VPSPLPKHQI IREASNVRNI TKMKKYDSTF YLHRDHVNYE EYGVDSLLFS 

       250        260        270        280        290        300 
YPEDSIQKVA RYERVQFASS ISVKKPSQQN KHFSLHVILG AVVDPDTFHG ENPGIPYDEL 

       310        320        330        340        350        360 
ALKNGSKGMF VYLQEMKTPP LIIRGRSPSN YASSQRITVR TPSSVNSSQN STKRKMPSMA 

       370        380        390        400        410        420 
QPLNESCLNA RPSKRRSKVA LGAPNSGASI SPIKSRQSTP MEASKENEDP FFRPNKRVET 

       430        440        450        460        470        480 
LEHIQNKLGA LKNQCPDSSL KYPSSSSRGM EGCLEKEDLV YSSSFSVNMK QIELKPARSF 

       490        500        510        520        530        540 
EHENIFKVGS LAFKKINELP HENYDITIEK KSMEQNYLRP EIGSRSECKT SYGNELSLSN 

       550        560        570        580        590        600 
ISFSILPNSA ENFHLETALF PATEEDVPRT FSRILETGSF QNYYQKMDAE NADRVYSKGV 

       610        620 
KLIASGTLPS GIFNREELFE EDSFYKY 

« Hide

References

« Hide 'large scale' references
[1]"NDT80, a meiosis-specific gene required for exit from pachytene in Saccharomyces cerevisiae."
Xu L., Ajimura M., Padmore R., Klein C., Kleckner N.
Mol. Cell. Biol. 15:6572-6581(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION.
Strain: S288c / GRF88.
[2]"Complete nucleotide sequence of Saccharomyces cerevisiae chromosome VIII."
Johnston M., Andrews S., Brinkman R., Cooper J., Ding H., Dover J., Du Z., Favello A., Fulton L., Gattung S., Geisel C., Kirsten J., Kucaba T., Hillier L.W., Jier M., Johnston L., Langston Y., Latreille P. expand/collapse author list , Louis E.J., Macri C., Mardis E., Menezes S., Mouser L., Nhan M., Rifkin L., Riles L., St Peter H., Trevaskis E., Vaughan K., Vignati D., Wilcox L., Wohldman P., Waterston R., Wilson R., Vaudin M.
Science 265:2077-2082(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[3]Saccharomyces Genome Database
Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[4]"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae."
Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. expand/collapse author list , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J.
Genome Res. 17:536-543(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[5]"Gametogenesis in yeast is regulated by a transcriptional cascade dependent on Ndt80."
Chu S., Herskowitz I.
Mol. Cell 1:685-696(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, DNA-BINDING.
[6]"NDT80 and the meiotic recombination checkpoint regulate expression of middle sporulation-specific genes in Saccharomyces cerevisiae."
Hepworth S.R., Friesen H., Segall J.
Mol. Cell. Biol. 18:5750-5761(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, INDUCTION.
[7]"The pachytene checkpoint prevents accumulation and phosphorylation of the meiosis-specific transcription factor Ndt80."
Tung K.-S., Hong E.-J.E., Roeder G.S.
Proc. Natl. Acad. Sci. U.S.A. 97:12187-12192(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION, SUBCELLULAR LOCATION.
[8]"Regulation of the premiddle and middle phases of expression of the NDT80 gene during sporulation of Saccharomyces cerevisiae."
Pak J., Segall J.
Mol. Cell. Biol. 22:6417-6429(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: INDUCTION.
[9]"Phosphorylation and maximal activity of Saccharomyces cerevisiae meiosis-specific transcription factor Ndt80 is dependent on Ime2."
Sopko R., Raithatha S., Stuart D.T.
Mol. Cell. Biol. 22:7024-7040(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION BY IME2.
[10]"Control of landmark events in meiosis by the CDK Cdc28 and the meiosis-specific kinase Ime2."
Benjamin K.R., Zhang C., Shokat K.M., Herskowitz I.
Genes Dev. 17:1524-1539(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION BY IME2, INDUCTION.
[11]"Sum1 and Ndt80 proteins compete for binding to middle sporulation element sequences that control meiotic gene expression."
Pierce M., Benjamin K.R., Montano S.P., Georgiadis M.M., Winter E., Vershon A.K.
Mol. Cell. Biol. 23:4814-4825(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[12]"Activity of phosphoforms and truncated versions of Ndt80, a checkpoint-regulated sporulation-specific transcription factor of Saccharomyces cerevisiae."
Shubassi G., Luca N., Pak J., Segall J.
Mol. Genet. Genomics 270:324-336(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION, INDUCTION.
[13]"Characterization of critical interactions between Ndt80 and MSE DNA defining a novel family of Ig-fold transcription factors."
Fingerman I.M., Sutphen K., Montano S.P., Georgiadis M.M., Vershon A.K.
Nucleic Acids Res. 32:2947-2956(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: DNA-BINDING, MUTAGENESIS OF LYS-50; LYS-54; 57-PRO--SER-59; ARG-97; ARG-111; TYR-113; 176-LYS-ARG-177; ARG-208; ARG-254 AND ARG-326.
[14]"Purification and characterization of the DNA binding domain of Saccharomyces cerevisiae meiosis-specific transcription factor Ndt80."
Sopko R., Stuart D.T.
Protein Expr. Purif. 33:134-144(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION BY IME2, DNA-BINDING.
[15]"Structure of the sporulation-specific transcription factor Ndt80 bound to DNA."
Lamoureux J.S., Stuart D.T., Tsang R., Wu C., Glover J.N.M.
EMBO J. 21:5721-5732(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (1.4 ANGSTROMS) OF 1-340 IN COMPLEX WITH DNA.
[16]"Crystal structure of the DNA-binding domain from Ndt80, a transcriptional activator required for meiosis in yeast."
Montano S.P., Cote M.L., Fingerman I.M., Pierce M., Vershon A.K., Georgiadis M.M.
Proc. Natl. Acad. Sci. U.S.A. 99:14041-14046(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (2.3 ANGSTROMS) OF 59-330, MUTAGENESIS OF LYS-110; ARG-111; TYR-113; HIS-173; LYS-176; ARG-177; ARG-202; ARG-208; ARG-254 AND ARG-326, SUBUNIT.
[17]"Principles of protein-DNA recognition revealed in the structural analysis of Ndt80-MSE DNA complexes."
Lamoureux J.S., Glover J.N.M.
Structure 14:555-565(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (1.55 ANGSTROMS) OF 1-340 IN COMPLEXES WITH TARGET DNA.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U35122 Genomic DNA. Translation: AAA92299.1.
U10398 Genomic DNA. Translation: AAB68408.1.
AY723825 Genomic DNA. Translation: AAU09742.1.
BK006934 Genomic DNA. Translation: DAA06818.1.
PIRS48968.
RefSeqNP_011992.1. NM_001179254.1.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1M6UX-ray2.30A/B59-330[»]
1M7UX-ray2.80A/B59-330[»]
1MN4X-ray2.20A59-340[»]
1MNNX-ray1.40A1-340[»]
2ETWX-ray1.67A1-340[»]
2EUVX-ray1.94A1-340[»]
2EUWX-ray1.68A1-340[»]
2EUXX-ray1.57A1-340[»]
2EUZX-ray1.56A1-340[»]
2EVFX-ray1.56A1-340[»]
2EVGX-ray1.55A1-340[»]
2EVHX-ray1.99A1-340[»]
2EVIX-ray1.80A1-340[»]
2EVJX-ray1.89A1-340[»]
ProteinModelPortalP38830.
SMRP38830. Positions 33-335.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid36557. 48 interactions.
IntActP38830. 3 interactions.
MINTMINT-2780712.
STRING4932.YHR124W.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYHR124W; YHR124W; YHR124W.
GeneID856524.
KEGGsce:YHR124W.

Organism-specific databases

SGDS000001166. NDT80.

Phylogenomic databases

eggNOGNOG43588.
HOGENOMHOG000066112.
KOK12769.
OrthoDBEOG7CCC1W.

Enzyme and pathway databases

BioCycYEAST:G3O-31165-MONOMER.

Gene expression databases

GenevestigatorP38830.

Family and domain databases

Gene3D2.60.40.1390. 1 hit.
InterProIPR024061. NDT80_DNA-bd_dom.
IPR008967. p53-like_TF_DNA-bd.
[Graphical view]
PfamPF05224. NDT80_PhoG. 1 hit.
[Graphical view]
SUPFAMSSF49417. SSF49417. 1 hit.
PROSITEPS51517. NDT80. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

EvolutionaryTraceP38830.
NextBio982290.

Entry information

Entry nameNDT80_YEAST
AccessionPrimary (citable) accession number: P38830
Secondary accession number(s): D3DL74, Q66R83
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: June 15, 2010
Last modified: February 19, 2014
This is version 114 of the entry and version 4 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome VIII

Yeast (Saccharomyces cerevisiae) chromosome VIII: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references