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Protein

Meiosis-specific transcription factor NDT80

Gene

NDT80

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Transcription factor required for successful completion of meiosis and spore formation. Gets activated after completion of meiotic recombination at the end of prophase I. Recognizes and binds to the middle sporulation element (MSE) 5'-C[AG]CAAA[AT]-3' in the promoter region of stage-specific genes that are required for progression through meiosis and sporulation. Competes for binding to MSE with the transcriptional repressor SUM1, which represses middle sporulation-specific genes during mitosis and early sporulation.4 Publications

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
DNA bindingi28 – 335NDT80PROSITE-ProRule annotationAdd BLAST308

GO - Molecular functioni

  • DNA binding Source: UniProtKB-KW
  • transcription factor activity, sequence-specific DNA binding Source: SGD

GO - Biological processi

  • cell division Source: UniProtKB-KW
  • meiotic nuclear division Source: SGD
  • positive regulation of transcription from RNA polymerase II promoter Source: SGD
  • transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Biological processi

Cell cycle, Cell division, Meiosis, Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-31165-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Meiosis-specific transcription factor NDT80
Gene namesi
Name:NDT80
Synonyms:DAS1
Ordered Locus Names:YHR124W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome VIII

Organism-specific databases

EuPathDBiFungiDB:YHR124W.
SGDiS000001166. NDT80.

Subcellular locationi

GO - Cellular componenti

  • nuclear chromosome Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi50K → A: Reduces DNA-binding by 70%. 1 Publication1
Mutagenesisi54K → A: Reduces DNA-binding by 50%. 1 Publication1
Mutagenesisi57P → A: Reduces DNA-binding by 65%. 1
Mutagenesisi58R → A: Reduces DNA-binding by 65%. 1
Mutagenesisi59S → A: Reduces DNA-binding by 86%. 1
Mutagenesisi97R → A: Reduces DNA-binding by 67%. 1 Publication1
Mutagenesisi110K → A: No effect on DNA-binding but strongly reduces progress through meiosis and sporulation. 1 Publication1
Mutagenesisi111R → A: Reduces DNA-binding by 95% and abolishes sporulation. 2 Publications1
Mutagenesisi113Y → A: Reduces DNA-binding by 80% and abolishes sporulation. 2 Publications1
Mutagenesisi173H → A: Reduces DNA-binding by 80% and strongly reduces progress through meiosis and sporulation. 1 Publication1
Mutagenesisi176K → A: Reduces DNA-binding by 50% but does not abolish sporulation. 1 Publication1
Mutagenesisi177R → A: Reduces DNA-binding by 96% and abolishes sporulation. 1 Publication1
Mutagenesisi202R → A: No effect on DNA-binding but strongly reduces progress through meiosis and sporulation. 1 Publication1
Mutagenesisi208R → A: Reduces DNA-binding by 50% and abolishes sporulation. 2 Publications1
Mutagenesisi254R → A: Reduces DNA-binding by 93% and abolishes sporulation. 2 Publications1
Mutagenesisi326R → A: Reduces DNA-binding by 50% and abolishes sporulation. 2 Publications1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000967721 – 627Meiosis-specific transcription factor NDT80Add BLAST627

Post-translational modificationi

Phosphorylated by pachytene checkpoint kinase IME2, but also phosphorylated in an IME2-independent manner. Phosphorylation probably eliminates SUM1-mediated repression and is also required for full transcriptional activation activity. Phosphorylation of the DNA-binding domain by IME2 does not alter DNA binding affinity.5 Publications

Keywords - PTMi

Phosphoprotein

Proteomic databases

PRIDEiP38830.

Expressioni

Inductioni

Induced during prophase I of meiosis. Requires protein kinase IME2 for initial expression and continued transcription during meiotic divisions. Can autoactivate its own synthesis.4 Publications

Interactioni

Subunit structurei

Binds to DNA as a monomer.2 Publications

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei58Interaction with DNA1
Sitei111Interaction with DNA1
Sitei177Interaction with DNA1
Sitei208Interaction with DNA1
Sitei254Interaction with DNA1
Sitei326Interaction with DNA1

Protein-protein interaction databases

BioGridi36557. 51 interactors.
IntActiP38830. 3 interactors.
MINTiMINT-2780712.

Structurei

Secondary structure

1627
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi35 – 38Combined sources4
Beta strandi44 – 47Combined sources4
Turni50 – 52Combined sources3
Helixi60 – 62Combined sources3
Beta strandi70 – 74Combined sources5
Beta strandi78 – 80Combined sources3
Turni81 – 83Combined sources3
Beta strandi90 – 98Combined sources9
Beta strandi100 – 102Combined sources3
Beta strandi105 – 109Combined sources5
Beta strandi114 – 121Combined sources8
Helixi127 – 132Combined sources6
Beta strandi135 – 137Combined sources3
Turni141 – 143Combined sources3
Beta strandi147 – 149Combined sources3
Beta strandi152 – 161Combined sources10
Turni162 – 164Combined sources3
Beta strandi166 – 168Combined sources3
Beta strandi170 – 173Combined sources4
Helixi177 – 179Combined sources3
Beta strandi188 – 190Combined sources3
Helixi198 – 203Combined sources6
Turni204 – 206Combined sources3
Helixi210 – 220Combined sources11
Beta strandi221 – 223Combined sources3
Helixi224 – 226Combined sources3
Helixi229 – 231Combined sources3
Helixi237 – 240Combined sources4
Beta strandi241 – 257Combined sources17
Beta strandi273 – 283Combined sources11
Helixi285 – 287Combined sources3
Beta strandi288 – 291Combined sources4
Beta strandi297 – 301Combined sources5
Beta strandi303 – 317Combined sources15
Beta strandi321 – 324Combined sources4
Helixi328 – 330Combined sources3
Helixi332 – 334Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1M6UX-ray2.30A/B59-330[»]
1M7UX-ray2.80A/B59-330[»]
1MN4X-ray2.20A59-340[»]
1MNNX-ray1.40A1-340[»]
2ETWX-ray1.67A1-340[»]
2EUVX-ray1.94A1-340[»]
2EUWX-ray1.68A1-340[»]
2EUXX-ray1.57A1-340[»]
2EUZX-ray1.56A1-340[»]
2EVFX-ray1.56A1-340[»]
2EVGX-ray1.55A1-340[»]
2EVHX-ray1.99A1-340[»]
2EVIX-ray1.80A1-340[»]
2EVJX-ray1.89A1-340[»]
ProteinModelPortaliP38830.
SMRiP38830.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP38830.

Family & Domainsi

Sequence similaritiesi

Contains 1 NDT80 DNA-binding domain.PROSITE-ProRule annotation

Phylogenomic databases

HOGENOMiHOG000066112.
InParanoidiP38830.
KOiK12769.
OrthoDBiEOG092C3Q5X.

Family and domain databases

Gene3Di2.60.40.1390. 1 hit.
InterProiIPR024061. NDT80_DNA-bd_dom.
IPR008967. p53-like_TF_DNA-bd.
[Graphical view]
PfamiPF05224. NDT80_PhoG. 1 hit.
[Graphical view]
SUPFAMiSSF49417. SSF49417. 1 hit.
PROSITEiPS51517. NDT80. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P38830-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNEMENTDPV LQDDLVSKYE RELSTEQEED TPVILTQLNE DGTTSNYFDK
60 70 80 90 100
RKLKIAPRST LQFKVGPPFE LVRDYCPVVE SHTGRTLDLR IIPRIDRGFD
110 120 130 140 150
HIDEEWVGYK RNYFTLVSTF ETANCDLDTF LKSSFDLLVE DSSVESRLRV
160 170 180 190 200
QYFAIKIKAK NDDDDTEINL VQHTAKRDKG PQFCPSVCPL VPSPLPKHQI
210 220 230 240 250
IREASNVRNI TKMKKYDSTF YLHRDHVNYE EYGVDSLLFS YPEDSIQKVA
260 270 280 290 300
RYERVQFASS ISVKKPSQQN KHFSLHVILG AVVDPDTFHG ENPGIPYDEL
310 320 330 340 350
ALKNGSKGMF VYLQEMKTPP LIIRGRSPSN YASSQRITVR TPSSVNSSQN
360 370 380 390 400
STKRKMPSMA QPLNESCLNA RPSKRRSKVA LGAPNSGASI SPIKSRQSTP
410 420 430 440 450
MEASKENEDP FFRPNKRVET LEHIQNKLGA LKNQCPDSSL KYPSSSSRGM
460 470 480 490 500
EGCLEKEDLV YSSSFSVNMK QIELKPARSF EHENIFKVGS LAFKKINELP
510 520 530 540 550
HENYDITIEK KSMEQNYLRP EIGSRSECKT SYGNELSLSN ISFSILPNSA
560 570 580 590 600
ENFHLETALF PATEEDVPRT FSRILETGSF QNYYQKMDAE NADRVYSKGV
610 620
KLIASGTLPS GIFNREELFE EDSFYKY
Length:627
Mass (Da):71,479
Last modified:June 15, 2010 - v4
Checksum:i947DA0BA08065595
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti200I → T (PubMed:12411490).Curated1
Sequence conflicti213M → T (PubMed:12384578).Curated1
Sequence conflicti225D → N (PubMed:12384578).Curated1
Sequence conflicti267S → F (PubMed:12384578).Curated1
Sequence conflicti363L → S in AAU09742 (PubMed:17322287).Curated1
Sequence conflicti493F → L in AAA92299 (PubMed:8524222).Curated1
Sequence conflicti568P → N in AAA92299 (PubMed:8524222).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U35122 Genomic DNA. Translation: AAA92299.1.
U10398 Genomic DNA. Translation: AAB68408.1.
AY723825 Genomic DNA. Translation: AAU09742.1.
BK006934 Genomic DNA. Translation: DAA06818.1.
PIRiS48968.
RefSeqiNP_011992.1. NM_001179254.1.

Genome annotation databases

EnsemblFungiiYHR124W; YHR124W; YHR124W.
GeneIDi856524.
KEGGisce:YHR124W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U35122 Genomic DNA. Translation: AAA92299.1.
U10398 Genomic DNA. Translation: AAB68408.1.
AY723825 Genomic DNA. Translation: AAU09742.1.
BK006934 Genomic DNA. Translation: DAA06818.1.
PIRiS48968.
RefSeqiNP_011992.1. NM_001179254.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1M6UX-ray2.30A/B59-330[»]
1M7UX-ray2.80A/B59-330[»]
1MN4X-ray2.20A59-340[»]
1MNNX-ray1.40A1-340[»]
2ETWX-ray1.67A1-340[»]
2EUVX-ray1.94A1-340[»]
2EUWX-ray1.68A1-340[»]
2EUXX-ray1.57A1-340[»]
2EUZX-ray1.56A1-340[»]
2EVFX-ray1.56A1-340[»]
2EVGX-ray1.55A1-340[»]
2EVHX-ray1.99A1-340[»]
2EVIX-ray1.80A1-340[»]
2EVJX-ray1.89A1-340[»]
ProteinModelPortaliP38830.
SMRiP38830.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi36557. 51 interactors.
IntActiP38830. 3 interactors.
MINTiMINT-2780712.

Proteomic databases

PRIDEiP38830.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYHR124W; YHR124W; YHR124W.
GeneIDi856524.
KEGGisce:YHR124W.

Organism-specific databases

EuPathDBiFungiDB:YHR124W.
SGDiS000001166. NDT80.

Phylogenomic databases

HOGENOMiHOG000066112.
InParanoidiP38830.
KOiK12769.
OrthoDBiEOG092C3Q5X.

Enzyme and pathway databases

BioCyciYEAST:G3O-31165-MONOMER.

Miscellaneous databases

EvolutionaryTraceiP38830.
PROiP38830.

Family and domain databases

Gene3Di2.60.40.1390. 1 hit.
InterProiIPR024061. NDT80_DNA-bd_dom.
IPR008967. p53-like_TF_DNA-bd.
[Graphical view]
PfamiPF05224. NDT80_PhoG. 1 hit.
[Graphical view]
SUPFAMiSSF49417. SSF49417. 1 hit.
PROSITEiPS51517. NDT80. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiNDT80_YEAST
AccessioniPrimary (citable) accession number: P38830
Secondary accession number(s): D3DL74, Q66R83
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: June 15, 2010
Last modified: November 2, 2016
This is version 134 of the entry and version 4 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome VIII
    Yeast (Saccharomyces cerevisiae) chromosome VIII: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.