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P38830

- NDT80_YEAST

UniProt

P38830 - NDT80_YEAST

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Protein

Meiosis-specific transcription factor NDT80

Gene

NDT80

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Transcription factor required for successful completion of meiosis and spore formation. Gets activated after completion of meiotic recombination at the end of prophase I. Recognizes and binds to the middle sporulation element (MSE) 5'-C[AG]CAAA[AT]-3' in the promoter region of stage-specific genes that are required for progression through meiosis and sporulation. Competes for binding to MSE with the transcriptional repressor SUM1, which represses middle sporulation-specific genes during mitosis and early sporulation.4 Publications

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei58 – 581Interaction with DNA
Sitei111 – 1111Interaction with DNA
Sitei177 – 1771Interaction with DNA
Sitei208 – 2081Interaction with DNA
Sitei254 – 2541Interaction with DNA
Sitei326 – 3261Interaction with DNA

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
DNA bindingi28 – 335308NDT80PROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

  1. DNA binding Source: UniProtKB-KW
  2. sequence-specific DNA binding transcription factor activity Source: SGD

GO - Biological processi

  1. meiotic nuclear division Source: SGD
  2. transcription, DNA-templated Source: SGD
Complete GO annotation...

Keywords - Biological processi

Cell cycle, Cell division, Meiosis, Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-31165-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Meiosis-specific transcription factor NDT80
Gene namesi
Name:NDT80
Synonyms:DAS1
Ordered Locus Names:YHR124W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome VIII

Organism-specific databases

SGDiS000001166. NDT80.

Subcellular locationi

Nucleus 1 Publication

GO - Cellular componenti

  1. nuclear chromosome Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi50 – 501K → A: Reduces DNA-binding by 70%. 1 Publication
Mutagenesisi54 – 541K → A: Reduces DNA-binding by 50%. 1 Publication
Mutagenesisi57 – 571P → A: Reduces DNA-binding by 65%.
Mutagenesisi58 – 581R → A: Reduces DNA-binding by 65%.
Mutagenesisi59 – 591S → A: Reduces DNA-binding by 86%.
Mutagenesisi97 – 971R → A: Reduces DNA-binding by 67%. 1 Publication
Mutagenesisi110 – 1101K → A: No effect on DNA-binding but strongly reduces progress through meiosis and sporulation. 1 Publication
Mutagenesisi111 – 1111R → A: Reduces DNA-binding by 95% and abolishes sporulation. 2 Publications
Mutagenesisi113 – 1131Y → A: Reduces DNA-binding by 80% and abolishes sporulation. 2 Publications
Mutagenesisi173 – 1731H → A: Reduces DNA-binding by 80% and strongly reduces progress through meiosis and sporulation. 1 Publication
Mutagenesisi176 – 1761K → A: Reduces DNA-binding by 50% but does not abolish sporulation. 1 Publication
Mutagenesisi177 – 1771R → A: Reduces DNA-binding by 96% and abolishes sporulation. 1 Publication
Mutagenesisi202 – 2021R → A: No effect on DNA-binding but strongly reduces progress through meiosis and sporulation. 1 Publication
Mutagenesisi208 – 2081R → A: Reduces DNA-binding by 50% and abolishes sporulation. 2 Publications
Mutagenesisi254 – 2541R → A: Reduces DNA-binding by 93% and abolishes sporulation. 2 Publications
Mutagenesisi326 – 3261R → A: Reduces DNA-binding by 50% and abolishes sporulation. 2 Publications

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 627627Meiosis-specific transcription factor NDT80PRO_0000096772Add
BLAST

Post-translational modificationi

Phosphorylated by pachytene checkpoint kinase IME2, but also phosphorylated in an IME2-independent manner. Phosphorylation probably eliminates SUM1-mediated repression and is also required for full transcriptional activation activity. Phosphorylation of the DNA-binding domain by IME2 does not alter DNA binding affinity.5 Publications

Keywords - PTMi

Phosphoprotein

Expressioni

Inductioni

Induced during prophase I of meiosis. Requires protein kinase IME2 for initial expression and continued transcription during meiotic divisions. Can autoactivate its own synthesis.4 Publications

Gene expression databases

GenevestigatoriP38830.

Interactioni

Subunit structurei

Binds to DNA as a monomer.2 Publications

Protein-protein interaction databases

BioGridi36557. 50 interactions.
IntActiP38830. 3 interactions.
MINTiMINT-2780712.
STRINGi4932.YHR124W.

Structurei

Secondary structure

1
627
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi35 – 384
Beta strandi44 – 474
Turni50 – 523
Helixi60 – 623
Beta strandi70 – 745
Beta strandi78 – 803
Turni81 – 833
Beta strandi90 – 989
Beta strandi100 – 1023
Beta strandi105 – 1095
Beta strandi114 – 1218
Helixi127 – 1326
Beta strandi135 – 1373
Turni141 – 1433
Beta strandi147 – 1493
Beta strandi152 – 16110
Turni162 – 1643
Beta strandi166 – 1683
Beta strandi170 – 1734
Helixi177 – 1793
Beta strandi188 – 1903
Helixi198 – 2036
Turni204 – 2063
Helixi210 – 22011
Beta strandi221 – 2233
Helixi224 – 2263
Helixi229 – 2313
Helixi237 – 2404
Beta strandi241 – 25717
Beta strandi273 – 28311
Helixi285 – 2873
Beta strandi288 – 2914
Beta strandi297 – 3015
Beta strandi303 – 31715
Beta strandi321 – 3244
Helixi328 – 3303
Helixi332 – 3343

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1M6UX-ray2.30A/B59-330[»]
1M7UX-ray2.80A/B59-330[»]
1MN4X-ray2.20A59-340[»]
1MNNX-ray1.40A1-340[»]
2ETWX-ray1.67A1-340[»]
2EUVX-ray1.94A1-340[»]
2EUWX-ray1.68A1-340[»]
2EUXX-ray1.57A1-340[»]
2EUZX-ray1.56A1-340[»]
2EVFX-ray1.56A1-340[»]
2EVGX-ray1.55A1-340[»]
2EVHX-ray1.99A1-340[»]
2EVIX-ray1.80A1-340[»]
2EVJX-ray1.89A1-340[»]
ProteinModelPortaliP38830.
SMRiP38830. Positions 33-335.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP38830.

Family & Domainsi

Sequence similaritiesi

Contains 1 NDT80 DNA-binding domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiNOG43588.
HOGENOMiHOG000066112.
InParanoidiP38830.
KOiK12769.
OrthoDBiEOG7CCC1W.

Family and domain databases

Gene3Di2.60.40.1390. 1 hit.
InterProiIPR024061. NDT80_DNA-bd_dom.
IPR008967. p53-like_TF_DNA-bd.
[Graphical view]
PfamiPF05224. NDT80_PhoG. 1 hit.
[Graphical view]
SUPFAMiSSF49417. SSF49417. 1 hit.
PROSITEiPS51517. NDT80. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P38830-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MNEMENTDPV LQDDLVSKYE RELSTEQEED TPVILTQLNE DGTTSNYFDK
60 70 80 90 100
RKLKIAPRST LQFKVGPPFE LVRDYCPVVE SHTGRTLDLR IIPRIDRGFD
110 120 130 140 150
HIDEEWVGYK RNYFTLVSTF ETANCDLDTF LKSSFDLLVE DSSVESRLRV
160 170 180 190 200
QYFAIKIKAK NDDDDTEINL VQHTAKRDKG PQFCPSVCPL VPSPLPKHQI
210 220 230 240 250
IREASNVRNI TKMKKYDSTF YLHRDHVNYE EYGVDSLLFS YPEDSIQKVA
260 270 280 290 300
RYERVQFASS ISVKKPSQQN KHFSLHVILG AVVDPDTFHG ENPGIPYDEL
310 320 330 340 350
ALKNGSKGMF VYLQEMKTPP LIIRGRSPSN YASSQRITVR TPSSVNSSQN
360 370 380 390 400
STKRKMPSMA QPLNESCLNA RPSKRRSKVA LGAPNSGASI SPIKSRQSTP
410 420 430 440 450
MEASKENEDP FFRPNKRVET LEHIQNKLGA LKNQCPDSSL KYPSSSSRGM
460 470 480 490 500
EGCLEKEDLV YSSSFSVNMK QIELKPARSF EHENIFKVGS LAFKKINELP
510 520 530 540 550
HENYDITIEK KSMEQNYLRP EIGSRSECKT SYGNELSLSN ISFSILPNSA
560 570 580 590 600
ENFHLETALF PATEEDVPRT FSRILETGSF QNYYQKMDAE NADRVYSKGV
610 620
KLIASGTLPS GIFNREELFE EDSFYKY
Length:627
Mass (Da):71,479
Last modified:June 15, 2010 - v4
Checksum:i947DA0BA08065595
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti200 – 2001I → T(PubMed:12411490)Curated
Sequence conflicti213 – 2131M → T(PubMed:12384578)Curated
Sequence conflicti225 – 2251D → N(PubMed:12384578)Curated
Sequence conflicti267 – 2671S → F(PubMed:12384578)Curated
Sequence conflicti363 – 3631L → S in AAU09742. (PubMed:17322287)Curated
Sequence conflicti493 – 4931F → L in AAA92299. (PubMed:8524222)Curated
Sequence conflicti568 – 5681P → N in AAA92299. (PubMed:8524222)Curated

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U35122 Genomic DNA. Translation: AAA92299.1.
U10398 Genomic DNA. Translation: AAB68408.1.
AY723825 Genomic DNA. Translation: AAU09742.1.
BK006934 Genomic DNA. Translation: DAA06818.1.
PIRiS48968.
RefSeqiNP_011992.1. NM_001179254.1.

Genome annotation databases

EnsemblFungiiYHR124W; YHR124W; YHR124W.
GeneIDi856524.
KEGGisce:YHR124W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U35122 Genomic DNA. Translation: AAA92299.1 .
U10398 Genomic DNA. Translation: AAB68408.1 .
AY723825 Genomic DNA. Translation: AAU09742.1 .
BK006934 Genomic DNA. Translation: DAA06818.1 .
PIRi S48968.
RefSeqi NP_011992.1. NM_001179254.1.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1M6U X-ray 2.30 A/B 59-330 [» ]
1M7U X-ray 2.80 A/B 59-330 [» ]
1MN4 X-ray 2.20 A 59-340 [» ]
1MNN X-ray 1.40 A 1-340 [» ]
2ETW X-ray 1.67 A 1-340 [» ]
2EUV X-ray 1.94 A 1-340 [» ]
2EUW X-ray 1.68 A 1-340 [» ]
2EUX X-ray 1.57 A 1-340 [» ]
2EUZ X-ray 1.56 A 1-340 [» ]
2EVF X-ray 1.56 A 1-340 [» ]
2EVG X-ray 1.55 A 1-340 [» ]
2EVH X-ray 1.99 A 1-340 [» ]
2EVI X-ray 1.80 A 1-340 [» ]
2EVJ X-ray 1.89 A 1-340 [» ]
ProteinModelPortali P38830.
SMRi P38830. Positions 33-335.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 36557. 50 interactions.
IntActi P38830. 3 interactions.
MINTi MINT-2780712.
STRINGi 4932.YHR124W.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YHR124W ; YHR124W ; YHR124W .
GeneIDi 856524.
KEGGi sce:YHR124W.

Organism-specific databases

SGDi S000001166. NDT80.

Phylogenomic databases

eggNOGi NOG43588.
HOGENOMi HOG000066112.
InParanoidi P38830.
KOi K12769.
OrthoDBi EOG7CCC1W.

Enzyme and pathway databases

BioCyci YEAST:G3O-31165-MONOMER.

Miscellaneous databases

EvolutionaryTracei P38830.
NextBioi 982290.

Gene expression databases

Genevestigatori P38830.

Family and domain databases

Gene3Di 2.60.40.1390. 1 hit.
InterProi IPR024061. NDT80_DNA-bd_dom.
IPR008967. p53-like_TF_DNA-bd.
[Graphical view ]
Pfami PF05224. NDT80_PhoG. 1 hit.
[Graphical view ]
SUPFAMi SSF49417. SSF49417. 1 hit.
PROSITEi PS51517. NDT80. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "NDT80, a meiosis-specific gene required for exit from pachytene in Saccharomyces cerevisiae."
    Xu L., Ajimura M., Padmore R., Klein C., Kleckner N.
    Mol. Cell. Biol. 15:6572-6581(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION.
    Strain: S288c / GRF88.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  4. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  5. "Gametogenesis in yeast is regulated by a transcriptional cascade dependent on Ndt80."
    Chu S., Herskowitz I.
    Mol. Cell 1:685-696(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, DNA-BINDING.
  6. "NDT80 and the meiotic recombination checkpoint regulate expression of middle sporulation-specific genes in Saccharomyces cerevisiae."
    Hepworth S.R., Friesen H., Segall J.
    Mol. Cell. Biol. 18:5750-5761(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INDUCTION.
  7. "The pachytene checkpoint prevents accumulation and phosphorylation of the meiosis-specific transcription factor Ndt80."
    Tung K.-S., Hong E.-J.E., Roeder G.S.
    Proc. Natl. Acad. Sci. U.S.A. 97:12187-12192(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION, SUBCELLULAR LOCATION.
  8. "Regulation of the premiddle and middle phases of expression of the NDT80 gene during sporulation of Saccharomyces cerevisiae."
    Pak J., Segall J.
    Mol. Cell. Biol. 22:6417-6429(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: INDUCTION.
  9. "Phosphorylation and maximal activity of Saccharomyces cerevisiae meiosis-specific transcription factor Ndt80 is dependent on Ime2."
    Sopko R., Raithatha S., Stuart D.T.
    Mol. Cell. Biol. 22:7024-7040(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION BY IME2.
  10. "Control of landmark events in meiosis by the CDK Cdc28 and the meiosis-specific kinase Ime2."
    Benjamin K.R., Zhang C., Shokat K.M., Herskowitz I.
    Genes Dev. 17:1524-1539(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION BY IME2, INDUCTION.
  11. "Sum1 and Ndt80 proteins compete for binding to middle sporulation element sequences that control meiotic gene expression."
    Pierce M., Benjamin K.R., Montano S.P., Georgiadis M.M., Winter E., Vershon A.K.
    Mol. Cell. Biol. 23:4814-4825(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  12. "Activity of phosphoforms and truncated versions of Ndt80, a checkpoint-regulated sporulation-specific transcription factor of Saccharomyces cerevisiae."
    Shubassi G., Luca N., Pak J., Segall J.
    Mol. Genet. Genomics 270:324-336(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION, INDUCTION.
  13. "Characterization of critical interactions between Ndt80 and MSE DNA defining a novel family of Ig-fold transcription factors."
    Fingerman I.M., Sutphen K., Montano S.P., Georgiadis M.M., Vershon A.K.
    Nucleic Acids Res. 32:2947-2956(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: DNA-BINDING, MUTAGENESIS OF LYS-50; LYS-54; 57-PRO--SER-59; ARG-97; ARG-111; TYR-113; 176-LYS-ARG-177; ARG-208; ARG-254 AND ARG-326.
  14. "Purification and characterization of the DNA binding domain of Saccharomyces cerevisiae meiosis-specific transcription factor Ndt80."
    Sopko R., Stuart D.T.
    Protein Expr. Purif. 33:134-144(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION BY IME2, DNA-BINDING.
  15. "Structure of the sporulation-specific transcription factor Ndt80 bound to DNA."
    Lamoureux J.S., Stuart D.T., Tsang R., Wu C., Glover J.N.M.
    EMBO J. 21:5721-5732(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (1.4 ANGSTROMS) OF 1-340 IN COMPLEX WITH DNA.
  16. "Crystal structure of the DNA-binding domain from Ndt80, a transcriptional activator required for meiosis in yeast."
    Montano S.P., Cote M.L., Fingerman I.M., Pierce M., Vershon A.K., Georgiadis M.M.
    Proc. Natl. Acad. Sci. U.S.A. 99:14041-14046(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.3 ANGSTROMS) OF 59-330, MUTAGENESIS OF LYS-110; ARG-111; TYR-113; HIS-173; LYS-176; ARG-177; ARG-202; ARG-208; ARG-254 AND ARG-326, SUBUNIT.
  17. "Principles of protein-DNA recognition revealed in the structural analysis of Ndt80-MSE DNA complexes."
    Lamoureux J.S., Glover J.N.M.
    Structure 14:555-565(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (1.55 ANGSTROMS) OF 1-340 IN COMPLEXES WITH TARGET DNA.

Entry informationi

Entry nameiNDT80_YEAST
AccessioniPrimary (citable) accession number: P38830
Secondary accession number(s): D3DL74, Q66R83
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: June 15, 2010
Last modified: October 29, 2014
This is version 118 of the entry and version 4 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome VIII
    Yeast (Saccharomyces cerevisiae) chromosome VIII: entries and gene names

External Data

Dasty 3