P38830 (NDT80_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Meiosis-specific transcription factor NDT80

Gene names

Name:	NDT80
Synonyms:	DAS1
Ordered Locus Names:	YHR124W

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › Saccharomyces cerevisiae

Protein attributes

Sequence length	627 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Transcription factor required for successful completion of meiosis and spore formation. Gets activated after completion of meiotic recombination at the end of prophase I. Recognizes and binds to the middle sporulation element (MSE) 5'-C[AG]CAAA[AT]-3' in the promoter region of stage-specific genes that are required for progression through meiosis and sporulation. Competes for binding to MSE with the transcriptional repressor SUM1, which represses middle sporulation-specific genes during mitosis and early sporulation. Ref.1 Ref.5 Ref.6 Ref.11
Subunit structure	Binds to DNA as a monomer. Ref.16
Subcellular location	Nucleus Ref.7.
Induction	Induced during prophase I of meiosis. Requires protein kinase IME2 for initial expression and continued transcription during meiotic divisions. Can autoactivate its own synthesis. Ref.6 Ref.8 Ref.10 Ref.12
Post-translational modification	Phosphorylated by pachytene checkpoint kinase IME2, but also phosphorylated in an IME2-independent manner. Phosphorylation probably eliminates SUM1-mediated repression and is also required for full transcriptional activation activity. Phosphorylation of the DNA-binding domain by IME2 does not alter DNA binding affinity. Ref.7 Ref.9 Ref.10 Ref.12 Ref.14
Sequence similarities	Contains 1 NDT80 DNA-binding domain.

Ontologies

Keywords
Biological process	Cell cycle Cell division Meiosis Transcription Transcription regulation
Cellular component	Nucleus
Ligand	DNA-binding
PTM	Phosphoprotein
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	cell division Inferred from electronic annotation. Source: UniProtKB-KW meiosis Inferred from mutant phenotype Ref.1Ref.5. Source: SGD transcription, DNA-dependent Inferred from genetic interaction Ref.5. Source: SGD
Cellular_component	nuclear chromosome Inferred from direct assay Ref.7. Source: SGD
Molecular_function	sequence-specific DNA binding Inferred from direct assay PubMed 19158363. Source: SGD sequence-specific DNA binding transcription factor activity Inferred from mutant phenotype Ref.5. Source: SGD
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 627

627

Meiosis-specific transcription factor NDT80

PRO_0000096772

Regions

DNA binding

28 – 335

308

NDT80 Ref.5 Ref.13 Ref.14

Sites

Site

58

1

Interaction with DNA

Site

111

1

Interaction with DNA

Site

177

1

Interaction with DNA

Site

208

1

Interaction with DNA

Site

254

1

Interaction with DNA

Site

326

1

Interaction with DNA

Experimental info

Mutagenesis

50

1

K → A: Reduces DNA-binding by 70%. Ref.13

Mutagenesis

54

1

K → A: Reduces DNA-binding by 50%. Ref.13

Mutagenesis

57

1

P → A: Reduces DNA-binding by 65%.

Mutagenesis

58

1

R → A: Reduces DNA-binding by 65%.

Mutagenesis

59

1

S → A: Reduces DNA-binding by 86%.

Mutagenesis

97

1

R → A: Reduces DNA-binding by 67%. Ref.13

Mutagenesis

110

1

K → A: No effect on DNA-binding but strongly reduces progress through meiosis and sporulation. Ref.16

Mutagenesis

111

1

R → A: Reduces DNA-binding by 95% and abolishes sporulation. Ref.13 Ref.16

Mutagenesis

113

1

Y → A: Reduces DNA-binding by 80% and abolishes sporulation. Ref.13 Ref.16

Mutagenesis

173

1

H → A: Reduces DNA-binding by 80% and strongly reduces progress through meiosis and sporulation. Ref.16

Mutagenesis

176

1

K → A: Reduces DNA-binding by 50% but does not abolish sporulation. Ref.16

Mutagenesis

177

1

R → A: Reduces DNA-binding by 96% and abolishes sporulation. Ref.16

Mutagenesis

202

1

R → A: No effect on DNA-binding but strongly reduces progress through meiosis and sporulation. Ref.16

Mutagenesis

208

1

R → A: Reduces DNA-binding by 50% and abolishes sporulation. Ref.13 Ref.16

Mutagenesis

254

1

R → A: Reduces DNA-binding by 93% and abolishes sporulation. Ref.13 Ref.16

Mutagenesis

326

1

R → A: Reduces DNA-binding by 50% and abolishes sporulation. Ref.13 Ref.16

Sequence conflict

200

1

I → T Ref.15

Sequence conflict

213

1

M → T Ref.16

Sequence conflict

225

1

D → N Ref.16

Sequence conflict

267

1

S → F Ref.16

Sequence conflict

363

1

L → S in AAU09742. Ref.4

Sequence conflict

493

1

F → L in AAA92299. Ref.1

Sequence conflict

568

1

P → N in AAA92299. Ref.1

Secondary structure

1

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627

Helix Strand Turn

Details...

Beta strand

35 – 38

4

Beta strand

44 – 47

4

Turn

50 – 52

3

Helix

60 – 62

3

Beta strand

70 – 74

5

Beta strand

78 – 80

3

Turn

81 – 83

3

Beta strand

90 – 98

9

Beta strand

100 – 102

3

Beta strand

105 – 109

5

Beta strand

114 – 121

8

Helix

127 – 132

6

Beta strand

135 – 137

3

Turn

141 – 143

3

Beta strand

147 – 149

3

Beta strand

152 – 161

10

Turn

162 – 164

3

Beta strand

166 – 168

3

Beta strand

170 – 173

4

Helix

177 – 179

3

Beta strand

188 – 190

3

Helix

198 – 203

6

Turn

204 – 206

3

Helix

210 – 220

11

Beta strand

221 – 223

3

Helix

224 – 226

3

Helix

229 – 231

3

Helix

237 – 240

4

Beta strand

241 – 257

17

Beta strand

273 – 283

11

Helix

285 – 287

3

Beta strand

288 – 291

4

Beta strand

297 – 301

5

Beta strand

303 – 317

15

Beta strand

321 – 324

4

Helix

328 – 330

3

Helix

332 – 334

3

Sequences

	Sequence		Length	Mass (Da)
	P38830 [UniParc]. Last modified June 15, 2010. Version 4. Checksum: 947DA0BA08065595	FASTA	627	71,479
	10 20 30 40 50 60 MNEMENTDPV LQDDLVSKYE RELSTEQEED TPVILTQLNE DGTTSNYFDK RKLKIAPRST 70 80 90 100 110 120 LQFKVGPPFE LVRDYCPVVE SHTGRTLDLR IIPRIDRGFD HIDEEWVGYK RNYFTLVSTF 130 140 150 160 170 180 ETANCDLDTF LKSSFDLLVE DSSVESRLRV QYFAIKIKAK NDDDDTEINL VQHTAKRDKG 190 200 210 220 230 240 PQFCPSVCPL VPSPLPKHQI IREASNVRNI TKMKKYDSTF YLHRDHVNYE EYGVDSLLFS 250 260 270 280 290 300 YPEDSIQKVA RYERVQFASS ISVKKPSQQN KHFSLHVILG AVVDPDTFHG ENPGIPYDEL 310 320 330 340 350 360 ALKNGSKGMF VYLQEMKTPP LIIRGRSPSN YASSQRITVR TPSSVNSSQN STKRKMPSMA 370 380 390 400 410 420 QPLNESCLNA RPSKRRSKVA LGAPNSGASI SPIKSRQSTP MEASKENEDP FFRPNKRVET 430 440 450 460 470 480 LEHIQNKLGA LKNQCPDSSL KYPSSSSRGM EGCLEKEDLV YSSSFSVNMK QIELKPARSF 490 500 510 520 530 540 EHENIFKVGS LAFKKINELP HENYDITIEK KSMEQNYLRP EIGSRSECKT SYGNELSLSN 550 560 570 580 590 600 ISFSILPNSA ENFHLETALF PATEEDVPRT FSRILETGSF QNYYQKMDAE NADRVYSKGV 610 620 KLIASGTLPS GIFNREELFE EDSFYKY

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"NDT80, a meiosis-specific gene required for exit from pachytene in Saccharomyces cerevisiae." Xu L., Ajimura M., Padmore R., Klein C., Kleckner N. Mol. Cell. Biol. 15:6572-6581(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION. Strain: S288c / GRF88.
[2]	"Complete nucleotide sequence of Saccharomyces cerevisiae chromosome VIII." Johnston M., Andrews S., Brinkman R., Cooper J., Ding H., Dover J., Du Z., Favello A., Fulton L., Gattung S., Geisel C., Kirsten J., Kucaba T., Hillier L.W., Jier M., Johnston L., Langston Y., Latreille P. , Louis E.J., Macri C., Mardis E., Menezes S., Mouser L., Nhan M., Rifkin L., Riles L., St Peter H., Trevaskis E., Vaughan K., Vignati D., Wilcox L., Wohldman P., Waterston R., Wilson R., Vaudin M. Science 265:2077-2082(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[3]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[4]	"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae." Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J. Genome Res. 17:536-543(2007) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[5]	"Gametogenesis in yeast is regulated by a transcriptional cascade dependent on Ndt80." Chu S., Herskowitz I. Mol. Cell 1:685-696(1998) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, DNA-BINDING.
[6]	"NDT80 and the meiotic recombination checkpoint regulate expression of middle sporulation-specific genes in Saccharomyces cerevisiae." Hepworth S.R., Friesen H., Segall J. Mol. Cell. Biol. 18:5750-5761(1998) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, INDUCTION.
[7]	"The pachytene checkpoint prevents accumulation and phosphorylation of the meiosis-specific transcription factor Ndt80." Tung K.-S., Hong E.-J.E., Roeder G.S. Proc. Natl. Acad. Sci. U.S.A. 97:12187-12192(2000) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION, SUBCELLULAR LOCATION.
[8]	"Regulation of the premiddle and middle phases of expression of the NDT80 gene during sporulation of Saccharomyces cerevisiae." Pak J., Segall J. Mol. Cell. Biol. 22:6417-6429(2002) [PubMed] [Europe PMC] [Abstract] Cited for: INDUCTION.
[9]	"Phosphorylation and maximal activity of Saccharomyces cerevisiae meiosis-specific transcription factor Ndt80 is dependent on Ime2." Sopko R., Raithatha S., Stuart D.T. Mol. Cell. Biol. 22:7024-7040(2002) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION BY IME2.
[10]	"Control of landmark events in meiosis by the CDK Cdc28 and the meiosis-specific kinase Ime2." Benjamin K.R., Zhang C., Shokat K.M., Herskowitz I. Genes Dev. 17:1524-1539(2003) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION BY IME2, INDUCTION.
[11]	"Sum1 and Ndt80 proteins compete for binding to middle sporulation element sequences that control meiotic gene expression." Pierce M., Benjamin K.R., Montano S.P., Georgiadis M.M., Winter E., Vershon A.K. Mol. Cell. Biol. 23:4814-4825(2003) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
[12]	"Activity of phosphoforms and truncated versions of Ndt80, a checkpoint-regulated sporulation-specific transcription factor of Saccharomyces cerevisiae." Shubassi G., Luca N., Pak J., Segall J. Mol. Genet. Genomics 270:324-336(2003) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION, INDUCTION.
[13]	"Characterization of critical interactions between Ndt80 and MSE DNA defining a novel family of Ig-fold transcription factors." Fingerman I.M., Sutphen K., Montano S.P., Georgiadis M.M., Vershon A.K. Nucleic Acids Res. 32:2947-2956(2004) [PubMed] [Europe PMC] [Abstract] Cited for: DNA-BINDING, MUTAGENESIS OF LYS-50; LYS-54; 57-PRO--SER-59; ARG-97; ARG-111; TYR-113; 176-LYS-ARG-177; ARG-208; ARG-254 AND ARG-326.
[14]	"Purification and characterization of the DNA binding domain of Saccharomyces cerevisiae meiosis-specific transcription factor Ndt80." Sopko R., Stuart D.T. Protein Expr. Purif. 33:134-144(2004) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION BY IME2, DNA-BINDING.
[15]	"Structure of the sporulation-specific transcription factor Ndt80 bound to DNA." Lamoureux J.S., Stuart D.T., Tsang R., Wu C., Glover J.N.M. EMBO J. 21:5721-5732(2002) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (1.4 ANGSTROMS) OF 1-340 IN COMPLEX WITH DNA.
[16]	"Crystal structure of the DNA-binding domain from Ndt80, a transcriptional activator required for meiosis in yeast." Montano S.P., Cote M.L., Fingerman I.M., Pierce M., Vershon A.K., Georgiadis M.M. Proc. Natl. Acad. Sci. U.S.A. 99:14041-14046(2002) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.3 ANGSTROMS) OF 59-330, MUTAGENESIS OF LYS-110; ARG-111; TYR-113; HIS-173; LYS-176; ARG-177; ARG-202; ARG-208; ARG-254 AND ARG-326, SUBUNIT.
[17]	"Principles of protein-DNA recognition revealed in the structural analysis of Ndt80-MSE DNA complexes." Lamoureux J.S., Glover J.N.M. Structure 14:555-565(2006) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (1.55 ANGSTROMS) OF 1-340 IN COMPLEXES WITH TARGET DNA.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

U35122 Genomic DNA. Translation: AAA92299.1.
U10398 Genomic DNA. Translation: AAB68408.1.
AY723825 Genomic DNA. Translation: AAU09742.1.
BK006934 Genomic DNA. Translation: DAA06818.1.

PIR

S48968.

RefSeq

NP_011992.1. NM_001179254.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1M6U	X-ray	2.30	A/B	59-330	[»]
1M7U	X-ray	2.80	A/B	59-330	[»]
1MN4	X-ray	2.20	A	59-340	[»]
1MNN	X-ray	1.40	A	1-340	[»]
2ETW	X-ray	1.67	A	1-340	[»]
2EUV	X-ray	1.94	A	1-340	[»]
2EUW	X-ray	1.68	A	1-340	[»]
2EUX	X-ray	1.57	A	1-340	[»]
2EUZ	X-ray	1.56	A	1-340	[»]
2EVF	X-ray	1.56	A	1-340	[»]
2EVG	X-ray	1.55	A	1-340	[»]
2EVH	X-ray	1.99	A	1-340	[»]
2EVI	X-ray	1.80	A	1-340	[»]
2EVJ	X-ray	1.89	A	1-340	[»]

ProteinModelPortal

P38830.

SMR

P38830. Positions 33-335.

ModBase

Search...

Protein-protein interaction databases

IntAct

P38830. 3 interactions.

MINT

MINT-2780712.

STRING

4932.YHR124W.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

EnsemblFungi

YHR124W; YHR124W; YHR124W.

GeneID

856524.

KEGG

sce:YHR124W.

Organism-specific databases

SGD

S000001166. NDT80.

Phylogenomic databases

eggNOG

NOG43588.

HOGENOM

HOG000066112.

KO

K12769.

OrthoDB

EOG45F0ZK.

Gene expression databases

Genevestigator

P38830.

GermOnline

YHR124W. Saccharomyces cerevisiae.

Family and domain databases

Gene3D

2.60.40.1390. 1 hit.

InterPro

IPR024061. NDT80_DNA-bd_dom.
IPR008967. p53-like_TF_DNA-bd.
[Graphical view]

Pfam

PF05224. NDT80_PhoG. 1 hit.
[Graphical view]

SUPFAM

SSF49417. P53_like_DNA_bnd. 1 hit.

PROSITE

PS51517. NDT80. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

EvolutionaryTrace

P38830.

NextBio

982290.

Entry information

Entry name

NDT80_YEAST

Accession

Primary (citable) accession number: P38830
Secondary accession number(s): D3DL74, Q66R83

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1995
Last sequence update:	June 15, 2010
Last modified:	May 1, 2013
This is version 107 of the entry and version 4 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program