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P38827

- SET1_YEAST

UniProt

P38827 - SET1_YEAST

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Protein

Histone-lysine N-methyltransferase, H3 lysine-4 specific

Gene

SET1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Catalytic component of the COMPASS (Set1C) complex that specifically mono-, di- and trimethylates histone H3 to form H3K4me1/2/3, which subsequently plays a role in telomere length maintenance and transcription elongation regulation.12 Publications

Catalytic activityi

S-adenosyl-L-methionine + L-lysine-[histone] = S-adenosyl-L-homocysteine + N(6)-methyl-L-lysine-[histone].1 Publication

GO - Molecular functioni

  1. histone-lysine N-methyltransferase activity Source: UniProtKB-EC
  2. histone methyltransferase activity Source: SGD
  3. protein-lysine N-methyltransferase activity Source: SGD
  4. RNA binding Source: SGD

GO - Biological processi

  1. ascospore formation Source: SGD
  2. chromatin silencing at rDNA Source: SGD
  3. chromatin silencing at silent mating-type cassette Source: SGD
  4. chromatin silencing at telomere Source: SGD
  5. histone H3-K4 methylation Source: SGD
  6. peptidyl-lysine dimethylation Source: SGD
  7. positive regulation of histone acetylation Source: SGD
  8. regulation of transcription from RNA polymerase II promoter in response to stress Source: SGD
  9. telomere maintenance Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Chromatin regulator, Methyltransferase, Transferase

Keywords - Ligandi

S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciYEAST:G3O-31161-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Histone-lysine N-methyltransferase, H3 lysine-4 specific (EC:2.1.1.43)
Alternative name(s):
COMPASS component SET1
Lysine N-methyltransferase 2
SET domain-containing protein 1
Gene namesi
Name:SET1
Synonyms:KMT2, YTX1
Ordered Locus Names:YHR119W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome VIII

Organism-specific databases

CYGDiYHR119w.
SGDiS000001161. SET1.

Subcellular locationi

Nucleus Curated. Chromosome Curated

GO - Cellular componenti

  1. chromosome Source: UniProtKB-KW
  2. Set1C/COMPASS complex Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Chromosome, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 10801080Histone-lysine N-methyltransferase, H3 lysine-4 specificPRO_0000186086Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei625 – 6251Phosphoserine2 Publications
Modified residuei875 – 8751Phosphothreonine1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP38827.
PaxDbiP38827.
PRIDEiP38827.

Expressioni

Gene expression databases

GenevestigatoriP38827.

Interactioni

Subunit structurei

Component of the COMPASS (Set1C) complex which consists of SET12, BRE22, SPP12, SDC11, SHG11, SWD11, SWD21, and SWD31. Interacts with MEC3.4 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
BRE2P431326EBI-16977,EBI-27115
MEC3Q025743EBI-16977,EBI-10658
SHG1P383375EBI-16977,EBI-21106
SWD2P361046EBI-16977,EBI-26608

Protein-protein interaction databases

BioGridi36552. 135 interactions.
DIPiDIP-4616N.
IntActiP38827. 30 interactions.
MINTiMINT-552558.
STRINGi4932.YHR119W.

Structurei

Secondary structure

1
1080
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi249 – 25810
Helixi265 – 2739
Beta strandi279 – 2857
Turni287 – 2893
Beta strandi292 – 2998
Helixi310 – 32011
Turni321 – 3244
Beta strandi326 – 3283
Beta strandi331 – 3377
Helixi342 – 36423

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2J8AX-ray3.00A247-375[»]
ProteinModelPortaliP38827.
SMRiP38827. Positions 247-366.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP38827.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini938 – 1055118SETPROSITE-ProRule annotationAdd
BLAST
Domaini1064 – 108017Post-SETPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the class V-like SAM-binding methyltransferase superfamily.PROSITE-ProRule annotation
Contains 1 post-SET domain.PROSITE-ProRule annotation
Contains 1 SET domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiCOG2940.
GeneTreeiENSGT00760000119228.
HOGENOMiHOG000066111.
InParanoidiP38827.
KOiK11422.
OMAiERLPCLC.
OrthoDBiEOG7P8PH3.

Family and domain databases

InterProiIPR024657. COMPASS_Set1_N-SET.
IPR017111. Hist_H3-K4_MeTrfase_1_fun.
IPR003616. Post-SET_dom.
IPR024636. SET_assoc.
IPR001214. SET_dom.
[Graphical view]
PfamiPF11764. N-SET. 1 hit.
PF00856. SET. 1 hit.
PF11767. SET_assoc. 1 hit.
[Graphical view]
PIRSFiPIRSF037104. Histone_H3-K4_mtfrase_Set1_fun. 1 hit.
SMARTiSM00508. PostSET. 1 hit.
SM00317. SET. 1 hit.
[Graphical view]
PROSITEiPS50868. POST_SET. 1 hit.
PS51572. SAM_MT43_1. 1 hit.
PS50280. SET. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P38827-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MSNYYRRAHA SSGSYRQPQE QPQYSRSGHY QYSNGHSHQQ YSSQYNQRRR
60 70 80 90 100
YNHNDGTRRR YNDDRPHSSN NASTRQYYAT NNSQSGPYVN KKSDISSRRG
110 120 130 140 150
MSQSRYSNSN VHNTLASSSG SLPTESALLL QQRPPSVLRY NTDNLKSKFH
160 170 180 190 200
YFDPIKGEFF NKDKMLSWKA TDKEFSETGY YVVKELQDGQ FKFKIKHRHP
210 220 230 240 250
EIKASDPRNE NGIMTSGKVA THRKCRNSLI LLPRISYDRY SLGPPPSCEI
260 270 280 290 300
VVYPAQDSTT TNIQDISIKN YFKKYGEISH FEAFNDPNSA LPLHVYLIKY
310 320 330 340 350
ASSDGKINDA AKAAFSAVRK HESSGCFIMG FKFEVILNKH SILNNIISKF
360 370 380 390 400
VEINVKKLQK LQENLKKAKE KEAENEKAKE LQGKDITLPK EPKVDTLSHS
410 420 430 440 450
SGSEKRIPYD LLGVVNNRPV LHVSKIFVAK HRFCVEDFKY KLRGYRCAKF
460 470 480 490 500
IDHPTGIYII FNDIAHAQTC SNAESGNLTI MSRSRRIPIL IKFHLILPRF
510 520 530 540 550
QNRTRFNKSS SSSNSTNVPI KYESKEEFIE ATAKQILKDL EKTLHVDIKK
560 570 580 590 600
RLIGPTVFDA LDHANFPELL AKRELKEKEK RQQIASKIAE DELKRKEEAK
610 620 630 640 650
RDFDLFGLYG GYAKSNKRNL KRHNSLALDH TSLKRKKLSN GIKPMAHLLN
660 670 680 690 700
EETDSKETTP LNDEGITRVS KEHDEEDENM TSSSSEEEEE EAPDKKFKSE
710 720 730 740 750
SEPTTPESDH LHGIKPLVPD QNGSSDVLDA SSMYKPTATE IPEPVYPPEE
760 770 780 790 800
YDLKYSQTLS SMDLQNAIKD EEDMLILKQL LSTYTPTVTP ETSAALEYKI
810 820 830 840 850
WQSRRKVLEE EKASDWQIEL NGTLFDSELQ PGSSFKAEGF RKIADKLKIN
860 870 880 890 900
YLPHRRRVHQ PLNTVNIHNE RNEYTPELCQ REESSNKEPS DSVPQEVSSS
910 920 930 940 950
RDNRASNRRF QQDIEAQKAA IGTESELLSL NQLNKRKKPV MFARSAIHNW
960 970 980 990 1000
GLYALDSIAA KEMIIEYVGE RIRQPVAEMR EKRYLKNGIG SSYLFRVDEN
1010 1020 1030 1040 1050
TVIDATKKGG IARFINHCCD PNCTAKIIKV GGRRRIVIYA LRDIAASEEL
1060 1070 1080
TYDYKFEREK DDEERLPCLC GAPNCKGFLN
Length:1,080
Mass (Da):123,912
Last modified:February 1, 1995 - v1
Checksum:iB7FA5D60F71063FD
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U00059 Genomic DNA. Translation: AAB68867.1.
BK006934 Genomic DNA. Translation: DAA06813.1.
PIRiS48961.
RefSeqiNP_011987.1. NM_001179249.1.

Genome annotation databases

EnsemblFungiiYHR119W; YHR119W; YHR119W.
GeneIDi856519.
KEGGisce:YHR119W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U00059 Genomic DNA. Translation: AAB68867.1 .
BK006934 Genomic DNA. Translation: DAA06813.1 .
PIRi S48961.
RefSeqi NP_011987.1. NM_001179249.1.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
2J8A X-ray 3.00 A 247-375 [» ]
ProteinModelPortali P38827.
SMRi P38827. Positions 247-366.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 36552. 135 interactions.
DIPi DIP-4616N.
IntActi P38827. 30 interactions.
MINTi MINT-552558.
STRINGi 4932.YHR119W.

Proteomic databases

MaxQBi P38827.
PaxDbi P38827.
PRIDEi P38827.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YHR119W ; YHR119W ; YHR119W .
GeneIDi 856519.
KEGGi sce:YHR119W.

Organism-specific databases

CYGDi YHR119w.
SGDi S000001161. SET1.

Phylogenomic databases

eggNOGi COG2940.
GeneTreei ENSGT00760000119228.
HOGENOMi HOG000066111.
InParanoidi P38827.
KOi K11422.
OMAi ERLPCLC.
OrthoDBi EOG7P8PH3.

Enzyme and pathway databases

BioCyci YEAST:G3O-31161-MONOMER.

Miscellaneous databases

EvolutionaryTracei P38827.
NextBioi 982275.
PROi P38827.

Gene expression databases

Genevestigatori P38827.

Family and domain databases

InterProi IPR024657. COMPASS_Set1_N-SET.
IPR017111. Hist_H3-K4_MeTrfase_1_fun.
IPR003616. Post-SET_dom.
IPR024636. SET_assoc.
IPR001214. SET_dom.
[Graphical view ]
Pfami PF11764. N-SET. 1 hit.
PF00856. SET. 1 hit.
PF11767. SET_assoc. 1 hit.
[Graphical view ]
PIRSFi PIRSF037104. Histone_H3-K4_mtfrase_Set1_fun. 1 hit.
SMARTi SM00508. PostSET. 1 hit.
SM00317. SET. 1 hit.
[Graphical view ]
PROSITEi PS50868. POST_SET. 1 hit.
PS51572. SAM_MT43_1. 1 hit.
PS50280. SET. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  2. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  3. "SET1, a yeast member of the Trithorax family, functions in transcriptional silencing and diverse cellular processes."
    Nislow C., Ray E., Pillus L.
    Mol. Biol. Cell 8:2421-2436(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  4. "Interaction between Set1p and checkpoint protein Mec3p in DNA repair and telomere functions."
    Corda Y., Schramke V., Longhese M.P., Smokvina T., Paciotti V., Brevet V., Gilson E., Geli V.
    Nat. Genet. 21:204-208(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH MEC3.
  5. "The Saccharomyces cerevisiae Set1 complex includes an Ash2 homologue and methylates histone 3 lysine 4."
    Roguev A., Schaft D., Shevchenko A., Pijnappel W.W.M.P., Wilm M., Aasland R., Stewart A.F.
    EMBO J. 20:7137-7148(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION IN THE SET1 COMPLEX, FUNCTION OF THE SET1 COMPLEX.
  6. "Histone H3 lysine 4 methylation is mediated by Set1 and required for cell growth and rDNA silencing in Saccharomyces cerevisiae."
    Briggs S.D., Bryk M., Strahl B.D., Cheung W.L., Davie J.K., Dent S.Y.R., Winston F., Allis C.D.
    Genes Dev. 15:3286-3295(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  7. Cited for: SUBUNIT.
  8. "Evidence that Set1, a factor required for methylation of histone H3, regulates rDNA silencing in S. cerevisiae by a Sir2-independent mechanism."
    Bryk M., Briggs S.D., Strahl B.D., Curcio M.J., Allis C.D., Winston F.
    Curr. Biol. 12:165-170(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  9. "COMPASS, a histone H3 (Lysine 4) methyltransferase required for telomeric silencing of gene expression."
    Krogan N.J., Dover J., Khorrami S., Greenblatt J.F., Schneider J., Johnston M., Shilatifard A.
    J. Biol. Chem. 277:10753-10755(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: ENZYME ACTIVITY, FUNCTION.
  10. Cited for: FUNCTION.
  11. "A trithorax-group complex purified from Saccharomyces cerevisiae is required for methylation of histone H3."
    Nagy P.L., Griesenbeck J., Kornberg R.D., Cleary M.L.
    Proc. Natl. Acad. Sci. U.S.A. 99:90-94(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION IN THE SET1 COMPLEX, FUNCTION OF THE SET1 COMPLEX.
  12. Cited for: FUNCTION.
  13. "Methylation of histone H3 K4 mediates association of the Isw1p ATPase with chromatin."
    Santos-Rosa H., Schneider R., Bernstein B.E., Karabetsou N., Morillon A., Weise C., Schreiber S.L., Mellor J., Kouzarides T.
    Mol. Cell 12:1325-1332(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  14. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  15. "Saccharomyces cerevisiae Set1p is a methyltransferase specific for lysine 4 of histone H3 and is required for efficient gene expression."
    Boa S., Coert C., Patterton H.-G.
    Yeast 20:827-835(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  16. "Dynamic lysine methylation on histone H3 defines the regulatory phase of gene transcription."
    Morillon A., Karabetsou N., Nair A., Mellor J.
    Mol. Cell 18:723-734(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION OF THE COMPASS COMPLEX.
  17. "Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
    Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
    J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-625, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Strain: ADR376.
  18. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
    Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
    Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-625 AND THR-875, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiSET1_YEAST
AccessioniPrimary (citable) accession number: P38827
Secondary accession number(s): D3DL69
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: February 1, 1995
Last modified: October 29, 2014
This is version 136 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 172 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome VIII
    Yeast (Saccharomyces cerevisiae) chromosome VIII: entries and gene names

External Data

Dasty 3