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P38826

- ORC6_YEAST

UniProt

P38826 - ORC6_YEAST

Protein

Origin recognition complex subunit 6

Gene

ORC6

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 125 (01 Oct 2014)
      Sequence version 2 (01 Nov 1995)
      Previous versions | rss
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    Functioni

    Component of the origin recognition complex (ORC) that binds origins of replication. It has a role in both chromosomal replication and mating type transcriptional silencing. Binds to the ARS consensus sequence (ACS) of origins of replication.2 Publications

    GO - Molecular functioni

    1. DNA replication origin binding Source: SGD
    2. protein binding Source: IntAct

    GO - Biological processi

    1. chromatin silencing at silent mating-type cassette Source: SGD
    2. DNA replication initiation Source: SGD
    3. pre-replicative complex assembly involved in nuclear cell cycle DNA replication Source: SGD

    Keywords - Biological processi

    DNA replication

    Keywords - Ligandi

    DNA-binding

    Enzyme and pathway databases

    BioCyciYEAST:G3O-31160-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Origin recognition complex subunit 6
    Alternative name(s):
    ACS-associated protein 1
    Origin recognition complex 50 kDa subunit
    Gene namesi
    Name:ORC6
    Synonyms:AAP1
    Ordered Locus Names:YHR118C
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome VIII

    Organism-specific databases

    CYGDiYHR118c.
    SGDiS000001160. ORC6.

    Subcellular locationi

    GO - Cellular componenti

    1. DNA replication preinitiation complex Source: SGD
    2. nuclear origin of replication recognition complex Source: SGD
    3. nuclear pre-replicative complex Source: SGD

    Keywords - Cellular componenti

    Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 435435Origin recognition complex subunit 6PRO_0000127102Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei146 – 1461Phosphothreonine1 Publication

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    MaxQBiP38826.
    PaxDbiP38826.

    Expressioni

    Gene expression databases

    GenevestigatoriP38826.

    Interactioni

    Subunit structurei

    Component of the origin recognition complex (ORC) composed of at least ORC1, ORC2, ORC3, ORC4, ORC5 and ORC6. Interacts with ORC3, ORC5 and TAH11.2 Publications

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    CDC14Q006842EBI-12588,EBI-4192
    CLB2P248692EBI-12588,EBI-4515
    ORC2P328336EBI-12588,EBI-12572
    ORC3P547904EBI-12588,EBI-12576

    Protein-protein interaction databases

    BioGridi36551. 68 interactions.
    DIPiDIP-2289N.
    IntActiP38826. 19 interactions.
    MINTiMINT-662807.
    STRINGi4932.YHR118C.

    Structurei

    3D structure databases

    ProteinModelPortaliP38826.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the ORC6 family.Curated

    Phylogenomic databases

    eggNOGiNOG38934.
    HOGENOMiHOG000000884.
    KOiK02608.
    OMAiARCHICA.
    OrthoDBiEOG7PS1S0.

    Family and domain databases

    InterProiIPR008721. ORC6.
    IPR016811. ORC6_fun.
    [Graphical view]
    PfamiPF05460. ORC6. 1 hit.
    [Graphical view]
    PIRSFiPIRSF022941. ORC6_fun. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    P38826-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSMQQVQHCV AEVLRLDPQE KPDWSSGYLK KLTNATSILY NTSLNKVMLK    50
    QDEEVARCHI CAYIASQKMN EKHMPDLCYY IDSIPLEPKK AKHLMNLFRQ 100
    SLSNSSPMKQ FAWTPSPKKN KRSPVKNGGR FTSSDPKELR NQLFGTPTKV 150
    RKSQNNDSFV IPELPPMQTN ESPSITRRKL AFEEDEDEDE EEPGNDGLSL 200
    KSHSNKSITG TRNVDSDEYE NHESDPTSEE EPLGVQESRS GRTKQNKAVG 250
    KPQSELKTAK ALRKRGRIPN SLLVKKYCKM TTEEIIRLCN DFELPREVAY 300
    KIVDEYNINA SRLVCPWQLV CGLVLNCTFI VFNERRRKDP RIDHFIVSKM 350
    CSLMLTSKVD DVIECVKLVK ELIIGEKWFR DLQIRYDDFD GIRYDEIIFR 400
    KLGSMLQTTN ILVTDDQYNI WKKRIEMDLA LTEPL 435
    Length:435
    Mass (Da):50,296
    Last modified:November 1, 1995 - v2
    Checksum:i22803C70378B7E85
    GO

    Sequence cautioni

    The sequence AAB68869.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L23323 Genomic DNA. Translation: AAA21822.1.
    U00059 Genomic DNA. Translation: AAB68869.1. Different initiation.
    BK006934 Genomic DNA. Translation: DAA06812.1.
    PIRiA49387.
    RefSeqiNP_011986.1. NM_001179248.1.

    Genome annotation databases

    EnsemblFungiiYHR118C; YHR118C; YHR118C.
    GeneIDi856518.
    KEGGisce:YHR118C.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L23323 Genomic DNA. Translation: AAA21822.1 .
    U00059 Genomic DNA. Translation: AAB68869.1 . Different initiation.
    BK006934 Genomic DNA. Translation: DAA06812.1 .
    PIRi A49387.
    RefSeqi NP_011986.1. NM_001179248.1.

    3D structure databases

    ProteinModelPortali P38826.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 36551. 68 interactions.
    DIPi DIP-2289N.
    IntActi P38826. 19 interactions.
    MINTi MINT-662807.
    STRINGi 4932.YHR118C.

    Proteomic databases

    MaxQBi P38826.
    PaxDbi P38826.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YHR118C ; YHR118C ; YHR118C .
    GeneIDi 856518.
    KEGGi sce:YHR118C.

    Organism-specific databases

    CYGDi YHR118c.
    SGDi S000001160. ORC6.

    Phylogenomic databases

    eggNOGi NOG38934.
    HOGENOMi HOG000000884.
    KOi K02608.
    OMAi ARCHICA.
    OrthoDBi EOG7PS1S0.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-31160-MONOMER.

    Miscellaneous databases

    NextBioi 982272.

    Gene expression databases

    Genevestigatori P38826.

    Family and domain databases

    InterProi IPR008721. ORC6.
    IPR016811. ORC6_fun.
    [Graphical view ]
    Pfami PF05460. ORC6. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF022941. ORC6_fun. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Isolation of ORC6, a component of the yeast origin recognition complex by a one-hybrid system."
      Li J.J., Herskowitz I.
      Science 262:1870-1874(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PARTIAL PROTEIN SEQUENCE.
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    3. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    4. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
    5. "Interaction between ORC and Cdt1p of Saccharomyces cerevisiae."
      Asano T., Makise M., Takehara M., Mizushima T.
      FEMS Yeast Res. 7:1256-1262(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, INTERACTION WITH TAH11.
    6. "Orc6 is required for dynamic recruitment of Cdt1 during repeated Mcm2-7 loading."
      Chen S., de Vries M.A., Bell S.P.
      Genes Dev. 21:2897-2907(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, INTERACTION WITH ORC3; ORC5 AND TAH11.
    7. "Analysis of phosphorylation sites on proteins from Saccharomyces cerevisiae by electron transfer dissociation (ETD) mass spectrometry."
      Chi A., Huttenhower C., Geer L.Y., Coon J.J., Syka J.E.P., Bai D.L., Shabanowitz J., Burke D.J., Troyanskaya O.G., Hunt D.F.
      Proc. Natl. Acad. Sci. U.S.A. 104:2193-2198(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    8. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
      Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
      Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-146, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    9. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
      Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
      Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiORC6_YEAST
    AccessioniPrimary (citable) accession number: P38826
    Secondary accession number(s): D3DL68
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 1, 1995
    Last sequence update: November 1, 1995
    Last modified: October 1, 2014
    This is version 125 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 2970 molecules/cell in log phase SD medium.1 Publication

    Caution

    It is uncertain whether Met-1 or Met-3 is the initiator.Curated

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families
    2. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    3. Yeast chromosome VIII
      Yeast (Saccharomyces cerevisiae) chromosome VIII: entries and gene names

    External Data

    Dasty 3