Origin recognition complex subunit 6 - Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

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P38826 (ORC6_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 114. History...

Clusters with 100%, 90%, 50% identity |

Documents (3) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Origin recognition complex subunit 6

Alternative name(s):
ACS-associated protein 1
Origin recognition complex 50 kDa subunit

Gene names

Name:	ORC6
Synonyms:	AAP1
Ordered Locus Names:	YHR118C

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces ›

Protein attributes

Sequence length	435 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Component of the origin recognition complex (ORC) that binds origins of replication. It has a role in both chromosomal replication and mating type transcriptional silencing. Binds to the ARS consensus sequence (ACS) of origins of replication. Ref.6 Ref.7
Subunit structure	Component of the origin recognition complex (ORC) composed of at least ORC1, ORC2, ORC3, ORC4, ORC5 and ORC6. Interacts with ORC3, ORC5 and TAH11. Ref.6 Ref.7
Subcellular location	Nucleus.
Miscellaneous	Present with 2970 molecules/cell in log phase SD medium.
Sequence similarities	Belongs to the ORC6 family.
Caution	It is uncertain whether Met-1 or Met-3 is the initiator.
Sequence caution	The sequence AAB68869.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.

Ontologies

Keywords
Biological process	DNA replication
Cellular component	Nucleus
Ligand	DNA-binding
PTM	Phosphoprotein
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	DNA replication initiation Inferred from mutant phenotype PubMed 17053779. Source: SGD chromatin silencing at silent mating-type cassette Inferred from direct assay PubMed 12897051. Source: SGD pre-replicative complex assembly Inferred from direct assay PubMed 16824194. Source: SGD
Cellular_component	DNA replication preinitiation complex Inferred from direct assay PubMed 9554851. Source: SGD nuclear origin of replication recognition complex Inferred from direct assay PubMed 9372948. Source: SGD pre-replicative complex Inferred from direct assay PubMed 16824194 PubMed 9335335. Source: SGD
Molecular_function	DNA replication origin binding Inferred from direct assay PubMed 16824194. Source: SGD
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct
CDC14	Q00684	2	EBI-12588,EBI-4192
CLB2	P24869	2	EBI-12588,EBI-4515
ORC2	P32833	6	EBI-12588,EBI-12572
ORC3	P54790	4	EBI-12588,EBI-12576

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 435

435

Origin recognition complex subunit 6

PRO_0000127102

Amino acid modifications

Modified residue

106

Phosphoserine Ref.8

Modified residue

146

Phosphothreonine Ref.5 Ref.10

Modified residue

216

Phosphoserine Ref.9

Sequences

Sequence

Length

Mass (Da)

Tools

P38826 [UniParc].

Last modified November 1, 1995. Version 2.
Checksum: 22803C70378B7E85
Show »

FASTA

435

50,296

        10         20         30         40         50         60 
MSMQQVQHCV AEVLRLDPQE KPDWSSGYLK KLTNATSILY NTSLNKVMLK QDEEVARCHI 

        70         80         90        100        110        120 
CAYIASQKMN EKHMPDLCYY IDSIPLEPKK AKHLMNLFRQ SLSNSSPMKQ FAWTPSPKKN 

       130        140        150        160        170        180 
KRSPVKNGGR FTSSDPKELR NQLFGTPTKV RKSQNNDSFV IPELPPMQTN ESPSITRRKL 

       190        200        210        220        230        240 
AFEEDEDEDE EEPGNDGLSL KSHSNKSITG TRNVDSDEYE NHESDPTSEE EPLGVQESRS 

       250        260        270        280        290        300 
GRTKQNKAVG KPQSELKTAK ALRKRGRIPN SLLVKKYCKM TTEEIIRLCN DFELPREVAY 

       310        320        330        340        350        360 
KIVDEYNINA SRLVCPWQLV CGLVLNCTFI VFNERRRKDP RIDHFIVSKM CSLMLTSKVD 

       370        380        390        400        410        420 
DVIECVKLVK ELIIGEKWFR DLQIRYDDFD GIRYDEIIFR KLGSMLQTTN ILVTDDQYNI 

       430 
WKKRIEMDLA LTEPL

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Isolation of ORC6, a component of the yeast origin recognition complex by a one-hybrid system." Li J.J., Herskowitz I. Science 262:1870-1874(1993) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PARTIAL PROTEIN SEQUENCE.
[2]	"Complete nucleotide sequence of Saccharomyces cerevisiae chromosome VIII." Johnston M., Andrews S., Brinkman R., Cooper J., Ding H., Dover J., Du Z., Favello A., Fulton L., Gattung S., Geisel C., Kirsten J., Kucaba T., Hillier L.W., Jier M., Johnston L., Langston Y., Latreille P. Vaudin M. Science 265:2077-2082(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[3]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[4]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[5]	"Quantitative phosphoproteomics applied to the yeast pheromone signaling pathway." Gruhler A., Olsen J.V., Mohammed S., Mortensen P., Faergeman N.J., Mann M., Jensen O.N. Mol. Cell. Proteomics 4:310-327(2005) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-146, MASS SPECTROMETRY. Strain: YAL6B.
[6]	"Interaction between ORC and Cdt1p of Saccharomyces cerevisiae." Asano T., Makise M., Takehara M., Mizushima T. FEMS Yeast Res. 7:1256-1262(2007) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, INTERACTION WITH TAH11.
[7]	"Orc6 is required for dynamic recruitment of Cdt1 during repeated Mcm2-7 loading." Chen S., de Vries M.A., Bell S.P. Genes Dev. 21:2897-2907(2007) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, INTERACTION WITH ORC3; ORC5 AND TAH11.
[8]	"Analysis of phosphorylation sites on proteins from Saccharomyces cerevisiae by electron transfer dissociation (ETD) mass spectrometry." Chi A., Huttenhower C., Geer L.Y., Coon J.J., Syka J.E.P., Bai D.L., Shabanowitz J., Burke D.J., Troyanskaya O.G., Hunt D.F. Proc. Natl. Acad. Sci. U.S.A. 104:2193-2198(2007) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-106, MASS SPECTROMETRY.
[9]	"Proteome-wide identification of in vivo targets of DNA damage checkpoint kinases." Smolka M.B., Albuquerque C.P., Chen S.H., Zhou H. Proc. Natl. Acad. Sci. U.S.A. 104:10364-10369(2007) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-216, MASS SPECTROMETRY.
[10]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-146, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	L23323 Genomic DNA. Translation: AAA21822.1. U00059 Genomic DNA. Translation: AAB68869.1. Different initiation. BK006934 Genomic DNA. Translation: DAA06812.1.
PIR	A49387.
RefSeq	NP_011986.1. NM_001179248.1.
3D structure databases
ProteinModelPortal	P38826.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-2289N.
IntAct	P38826. 19 interactions.
MINT	MINT-662807.
STRING	4932.YHR118C.
Proteomic databases
PaxDb	P38826.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YHR118C; YHR118C; YHR118C.
GeneID	856518.
KEGG	sce:YHR118C.
Organism-specific databases
CYGD	YHR118c.
SGD	S000001160. ORC6.
Phylogenomic databases
eggNOG	NOG38934.
HOGENOM	HOG000000884.
KO	K02608.
OMA	VARCHIC.
OrthoDB	EOG4MD18J.
Gene expression databases
Genevestigator	P38826.
GermOnline	YHR118C. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR008721. ORC6. IPR016811. ORC6_fun. [Graphical view]
Pfam	PF05460. ORC6. 1 hit. [Graphical view]
PIRSF	PIRSF022941. ORC6_fun. 1 hit.
ProtoNet	Search...
Other
NextBio	982272.

Entry information

Entry name

ORC6_YEAST

Accession

Primary (citable) accession number: P38826
Secondary accession number(s): D3DL68

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1995
Last sequence update:	November 1, 1995
Last modified:	May 1, 2013
This is version 114 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program

Relevant documents

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

Yeast chromosome VIII

Yeast (Saccharomyces cerevisiae) chromosome VIII: entries and gene names

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Binary interactions

With

Entry

#Exp.

IntAct

Notes

Sequence annotation (Features)

Molecule processing

Amino acid modifications

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents