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P38826

- ORC6_YEAST

UniProt

P38826 - ORC6_YEAST

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Protein

Origin recognition complex subunit 6

Gene

ORC6

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Component of the origin recognition complex (ORC) that binds origins of replication. It has a role in both chromosomal replication and mating type transcriptional silencing. Binds to the ARS consensus sequence (ACS) of origins of replication.2 Publications

GO - Molecular functioni

  1. DNA replication origin binding Source: SGD

GO - Biological processi

  1. chromatin silencing at silent mating-type cassette Source: SGD
  2. DNA replication initiation Source: SGD
  3. pre-replicative complex assembly involved in nuclear cell cycle DNA replication Source: SGD
Complete GO annotation...

Keywords - Biological processi

DNA replication

Keywords - Ligandi

DNA-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-31160-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Origin recognition complex subunit 6
Alternative name(s):
ACS-associated protein 1
Origin recognition complex 50 kDa subunit
Gene namesi
Name:ORC6
Synonyms:AAP1
Ordered Locus Names:YHR118C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome VIII

Organism-specific databases

CYGDiYHR118c.
SGDiS000001160. ORC6.

Subcellular locationi

GO - Cellular componenti

  1. DNA replication preinitiation complex Source: SGD
  2. nuclear origin of replication recognition complex Source: SGD
  3. nuclear pre-replicative complex Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 435435Origin recognition complex subunit 6PRO_0000127102Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei146 – 1461Phosphothreonine1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP38826.
PaxDbiP38826.

Expressioni

Gene expression databases

GenevestigatoriP38826.

Interactioni

Subunit structurei

Component of the origin recognition complex (ORC) composed of at least ORC1, ORC2, ORC3, ORC4, ORC5 and ORC6. Interacts with ORC3, ORC5 and TAH11.2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
CDC14Q006842EBI-12588,EBI-4192
CLB2P248692EBI-12588,EBI-4515
ORC2P328336EBI-12588,EBI-12572
ORC3P547904EBI-12588,EBI-12576

Protein-protein interaction databases

BioGridi36551. 68 interactions.
DIPiDIP-2289N.
IntActiP38826. 19 interactions.
MINTiMINT-662807.
STRINGi4932.YHR118C.

Structurei

3D structure databases

ProteinModelPortaliP38826.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the ORC6 family.Curated

Phylogenomic databases

eggNOGiNOG38934.
HOGENOMiHOG000000884.
InParanoidiP38826.
KOiK02608.
OMAiARCHICA.
OrthoDBiEOG7PS1S0.

Family and domain databases

InterProiIPR008721. ORC6.
IPR016811. ORC6_fun.
[Graphical view]
PfamiPF05460. ORC6. 1 hit.
[Graphical view]
PIRSFiPIRSF022941. ORC6_fun. 1 hit.

Sequencei

Sequence statusi: Complete.

P38826-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MSMQQVQHCV AEVLRLDPQE KPDWSSGYLK KLTNATSILY NTSLNKVMLK
60 70 80 90 100
QDEEVARCHI CAYIASQKMN EKHMPDLCYY IDSIPLEPKK AKHLMNLFRQ
110 120 130 140 150
SLSNSSPMKQ FAWTPSPKKN KRSPVKNGGR FTSSDPKELR NQLFGTPTKV
160 170 180 190 200
RKSQNNDSFV IPELPPMQTN ESPSITRRKL AFEEDEDEDE EEPGNDGLSL
210 220 230 240 250
KSHSNKSITG TRNVDSDEYE NHESDPTSEE EPLGVQESRS GRTKQNKAVG
260 270 280 290 300
KPQSELKTAK ALRKRGRIPN SLLVKKYCKM TTEEIIRLCN DFELPREVAY
310 320 330 340 350
KIVDEYNINA SRLVCPWQLV CGLVLNCTFI VFNERRRKDP RIDHFIVSKM
360 370 380 390 400
CSLMLTSKVD DVIECVKLVK ELIIGEKWFR DLQIRYDDFD GIRYDEIIFR
410 420 430
KLGSMLQTTN ILVTDDQYNI WKKRIEMDLA LTEPL
Length:435
Mass (Da):50,296
Last modified:November 1, 1995 - v2
Checksum:i22803C70378B7E85
GO

Sequence cautioni

The sequence AAB68869.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
L23323 Genomic DNA. Translation: AAA21822.1.
U00059 Genomic DNA. Translation: AAB68869.1. Different initiation.
BK006934 Genomic DNA. Translation: DAA06812.1.
PIRiA49387.
RefSeqiNP_011986.1. NM_001179248.1.

Genome annotation databases

EnsemblFungiiYHR118C; YHR118C; YHR118C.
GeneIDi856518.
KEGGisce:YHR118C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
L23323 Genomic DNA. Translation: AAA21822.1 .
U00059 Genomic DNA. Translation: AAB68869.1 . Different initiation.
BK006934 Genomic DNA. Translation: DAA06812.1 .
PIRi A49387.
RefSeqi NP_011986.1. NM_001179248.1.

3D structure databases

ProteinModelPortali P38826.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 36551. 68 interactions.
DIPi DIP-2289N.
IntActi P38826. 19 interactions.
MINTi MINT-662807.
STRINGi 4932.YHR118C.

Proteomic databases

MaxQBi P38826.
PaxDbi P38826.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YHR118C ; YHR118C ; YHR118C .
GeneIDi 856518.
KEGGi sce:YHR118C.

Organism-specific databases

CYGDi YHR118c.
SGDi S000001160. ORC6.

Phylogenomic databases

eggNOGi NOG38934.
HOGENOMi HOG000000884.
InParanoidi P38826.
KOi K02608.
OMAi ARCHICA.
OrthoDBi EOG7PS1S0.

Enzyme and pathway databases

BioCyci YEAST:G3O-31160-MONOMER.

Miscellaneous databases

NextBioi 982272.

Gene expression databases

Genevestigatori P38826.

Family and domain databases

InterProi IPR008721. ORC6.
IPR016811. ORC6_fun.
[Graphical view ]
Pfami PF05460. ORC6. 1 hit.
[Graphical view ]
PIRSFi PIRSF022941. ORC6_fun. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Isolation of ORC6, a component of the yeast origin recognition complex by a one-hybrid system."
    Li J.J., Herskowitz I.
    Science 262:1870-1874(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PARTIAL PROTEIN SEQUENCE.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  4. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  5. "Interaction between ORC and Cdt1p of Saccharomyces cerevisiae."
    Asano T., Makise M., Takehara M., Mizushima T.
    FEMS Yeast Res. 7:1256-1262(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH TAH11.
  6. "Orc6 is required for dynamic recruitment of Cdt1 during repeated Mcm2-7 loading."
    Chen S., de Vries M.A., Bell S.P.
    Genes Dev. 21:2897-2907(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH ORC3; ORC5 AND TAH11.
  7. "Analysis of phosphorylation sites on proteins from Saccharomyces cerevisiae by electron transfer dissociation (ETD) mass spectrometry."
    Chi A., Huttenhower C., Geer L.Y., Coon J.J., Syka J.E.P., Bai D.L., Shabanowitz J., Burke D.J., Troyanskaya O.G., Hunt D.F.
    Proc. Natl. Acad. Sci. U.S.A. 104:2193-2198(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  8. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
    Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
    Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-146, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  9. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
    Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
    Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiORC6_YEAST
AccessioniPrimary (citable) accession number: P38826
Secondary accession number(s): D3DL68
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: November 1, 1995
Last modified: October 29, 2014
This is version 126 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 2970 molecules/cell in log phase SD medium.1 Publication

Caution

It is uncertain whether Met-1 or Met-3 is the initiator.Curated

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome VIII
    Yeast (Saccharomyces cerevisiae) chromosome VIII: entries and gene names

External Data

Dasty 3