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Protein

Protein TOM71

Gene

TOM71

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Involved in MBF1-mediated mitochondrial morphogenesis.1 Publication

GO - Molecular functioni

  • protein channel activity Source: SGD

GO - Biological processi

  • protein targeting to mitochondrion Source: SGD
Complete GO annotation...

Enzyme and pathway databases

BioCyciYEAST:G3O-31159-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Protein TOM71
Alternative name(s):
71 kDa mitochondrial outer membrane protein
Gene namesi
Name:TOM71
Synonyms:TOM72
Ordered Locus Names:YHR117W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome VIII

Organism-specific databases

EuPathDBiFungiDB:YHR117W.
SGDiS000001159. TOM71.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 1414Mitochondrial intermembraneBy similarityAdd
BLAST
Transmembranei15 – 3218HelicalSequence analysisAdd
BLAST
Topological domaini33 – 639607CytoplasmicBy similarityAdd
BLAST

GO - Cellular componenti

  • integral component of mitochondrial outer membrane Source: SGD
  • mitochondrial outer membrane Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Membrane, Mitochondrion, Mitochondrion outer membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 639639Protein TOM71PRO_0000106420Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei55 – 551PhosphoserineCombined sources

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP38825.

PTM databases

iPTMnetiP38825.

Interactioni

Subunit structurei

Interacts with TOM40, TOM70 and MBF1.2 Publications

Protein-protein interaction databases

BioGridi36550. 39 interactions.
DIPiDIP-5618N.
IntActiP38825. 23 interactions.
MINTiMINT-512763.

Structurei

Secondary structure

1
639
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi112 – 1154Combined sources
Helixi120 – 13920Combined sources
Helixi147 – 15610Combined sources
Helixi161 – 17414Combined sources
Helixi177 – 19014Combined sources
Helixi195 – 20814Combined sources
Helixi211 – 22111Combined sources
Helixi235 – 25117Combined sources
Helixi266 – 2749Combined sources
Helixi278 – 2836Combined sources
Helixi295 – 30612Combined sources
Helixi310 – 33122Combined sources
Helixi338 – 35720Combined sources
Helixi361 – 37414Combined sources
Helixi378 – 38710Combined sources
Helixi395 – 40713Combined sources
Helixi412 – 42413Combined sources
Helixi428 – 44114Combined sources
Helixi447 – 45812Combined sources
Helixi462 – 47514Combined sources
Helixi481 – 49212Combined sources
Helixi496 – 51217Combined sources
Helixi521 – 53414Combined sources
Helixi543 – 55917Combined sources
Helixi564 – 57613Combined sources
Helixi580 – 59314Combined sources
Helixi598 – 61720Combined sources
Helixi620 – 6267Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3FP2X-ray1.98A107-639[»]
3FP3X-ray1.98A107-639[»]
3FP4X-ray2.14A107-639[»]
3LCAX-ray2.19A107-639[»]
ProteinModelPortaliP38825.
SMRiP38825. Positions 109-627.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP38825.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati127 – 16034TPR 1Add
BLAST
Repeati162 – 19433TPR 2Add
BLAST
Repeati345 – 37834TPR 3Add
BLAST
Repeati379 – 41133TPR 4Add
BLAST
Repeati412 – 44534TPR 5Add
BLAST
Repeati447 – 47933TPR 6Add
BLAST
Repeati480 – 51334TPR 7Add
BLAST
Repeati530 – 56334TPR 8Add
BLAST
Repeati565 – 59733TPR 9Add
BLAST

Sequence similaritiesi

Belongs to the Tom70 family.Curated
Contains 9 TPR repeats.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, TPR repeat, Transmembrane, Transmembrane helix

Phylogenomic databases

GeneTreeiENSGT00840000129775.
HOGENOMiHOG000074722.
InParanoidiP38825.
OMAiSSEADYK.
OrthoDBiEOG7R839F.

Family and domain databases

Gene3Di1.25.40.10. 3 hits.
InterProiIPR013026. TPR-contain_dom.
IPR011990. TPR-like_helical_dom.
IPR001440. TPR_1.
IPR019734. TPR_repeat.
[Graphical view]
PfamiPF00515. TPR_1. 2 hits.
PF13181. TPR_8. 2 hits.
[Graphical view]
SMARTiSM00028. TPR. 8 hits.
[Graphical view]
SUPFAMiSSF48452. SSF48452. 3 hits.
PROSITEiPS50005. TPR. 7 hits.
PS50293. TPR_REGION. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P38825-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAENSLLRFI TKNKVAILAT VSAGTAAVGA YVYYQQIKQQ QQQQLKGTKD
60 70 80 90 100
NRRQSEAFAG QNEDEADLKD DGSVVSGSNK RKKKKNKRKR NNKAKSGEGF
110 120 130 140 150
DYPSLPNGEP DIAQLKGLSP SQRQAYAVQL KNRGNHFFTA KNFNEAIKYY
160 170 180 190 200
QYAIELDPNE PVFYSNISAC YISTGDLEKV IEFTTKALEI KPDHSKALLR
210 220 230 240 250
RASANESLGN FTDAMFDLSV LSLNGDFDGA SIEPMLERNL NKQAMKVLNE
260 270 280 290 300
NLSKDEGRGS QVLPSNTSLA SFFGIFDSHL EVSSVNTSSN YDTAYALLSD
310 320 330 340 350
ALQRLYSATD EGYLVANDLL TKSTDMYHSL LSANTVDDPL RENAALALCY
360 370 380 390 400
TGIFHFLKNN LLDAQVLLQE SINLHPTPNS YIFLALTLAD KENSQEFFKF
410 420 430 440 450
FQKAVDLNPE YPPTYYHRGQ MYFILQDYKN AKEDFQKAQS LNPENVYPYI
460 470 480 490 500
QLACLLYKQG KFTESEAFFN ETKLKFPTLP EVPTFFAEIL TDRGDFDTAI
510 520 530 540 550
KQYDIAKRLE EVQEKIHVGI GPLIGKATIL ARQSSQDPTQ LDEEKFNAAI
560 570 580 590 600
KLLTKACELD PRSEQAKIGL AQLKLQMEKI DEAIELFEDS AILARTMDEK
610 620 630
LQATTFAEAA KIQKRLRADP IISAKMELTL ARYRAKGML
Length:639
Mass (Da):71,856
Last modified:February 1, 1995 - v1
Checksum:iB4AD583BB6CB466B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00059 Genomic DNA. Translation: AAB68868.1.
BK006934 Genomic DNA. Translation: DAA06811.1.
PIRiS48959.
RefSeqiNP_011985.1. NM_001179247.1.

Genome annotation databases

EnsemblFungiiYHR117W; YHR117W; YHR117W.
GeneIDi856517.
KEGGisce:YHR117W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00059 Genomic DNA. Translation: AAB68868.1.
BK006934 Genomic DNA. Translation: DAA06811.1.
PIRiS48959.
RefSeqiNP_011985.1. NM_001179247.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3FP2X-ray1.98A107-639[»]
3FP3X-ray1.98A107-639[»]
3FP4X-ray2.14A107-639[»]
3LCAX-ray2.19A107-639[»]
ProteinModelPortaliP38825.
SMRiP38825. Positions 109-627.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi36550. 39 interactions.
DIPiDIP-5618N.
IntActiP38825. 23 interactions.
MINTiMINT-512763.

PTM databases

iPTMnetiP38825.

Proteomic databases

MaxQBiP38825.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYHR117W; YHR117W; YHR117W.
GeneIDi856517.
KEGGisce:YHR117W.

Organism-specific databases

EuPathDBiFungiDB:YHR117W.
SGDiS000001159. TOM71.

Phylogenomic databases

GeneTreeiENSGT00840000129775.
HOGENOMiHOG000074722.
InParanoidiP38825.
OMAiSSEADYK.
OrthoDBiEOG7R839F.

Enzyme and pathway databases

BioCyciYEAST:G3O-31159-MONOMER.

Miscellaneous databases

EvolutionaryTraceiP38825.
PROiP38825.

Family and domain databases

Gene3Di1.25.40.10. 3 hits.
InterProiIPR013026. TPR-contain_dom.
IPR011990. TPR-like_helical_dom.
IPR001440. TPR_1.
IPR019734. TPR_repeat.
[Graphical view]
PfamiPF00515. TPR_1. 2 hits.
PF13181. TPR_8. 2 hits.
[Graphical view]
SMARTiSM00028. TPR. 8 hits.
[Graphical view]
SUPFAMiSSF48452. SSF48452. 3 hits.
PROSITEiPS50005. TPR. 7 hits.
PS50293. TPR_REGION. 2 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  2. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  3. "Identification of a third yeast mitochondrial Tom protein with tetratrico peptide repeats."
    Boemer U., Pfanner N., Dietmeier K.
    FEBS Lett. 382:153-158(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION.
  4. "Tom71, a novel homologue of the mitochondrial preprotein receptor Tom70."
    Schlossmann J., Lill R., Neupert W., Court D.A.
    J. Biol. Chem. 271:17890-17895(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH TOM40 AND TOM70, SUBCELLULAR LOCATION.
  5. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
  6. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  7. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
    Strain: ATCC 76625 / YPH499.
  8. "Integral membrane proteins in the mitochondrial outer membrane of Saccharomyces cerevisiae."
    Burri L., Vascotto K., Gentle I.E., Chan N.C., Beilharz T., Stapleton D.I., Ramage L., Lithgow T.
    FEBS J. 273:1507-1515(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION, IDENTIFICATION BY MASS SPECTROMETRY.
  9. "Proteomic analysis of the yeast mitochondrial outer membrane reveals accumulation of a subclass of preproteins."
    Zahedi R.P., Sickmann A., Boehm A.M., Winkler C., Zufall N., Schoenfisch B., Guiard B., Pfanner N., Meisinger C.
    Mol. Biol. Cell 17:1436-1450(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION, IDENTIFICATION BY MASS SPECTROMETRY.
  10. "Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
    Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
    J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-55, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Strain: ADR376.
  11. "Analysis of phosphorylation sites on proteins from Saccharomyces cerevisiae by electron transfer dissociation (ETD) mass spectrometry."
    Chi A., Huttenhower C., Geer L.Y., Coon J.J., Syka J.E.P., Bai D.L., Shabanowitz J., Burke D.J., Troyanskaya O.G., Hunt D.F.
    Proc. Natl. Acad. Sci. U.S.A. 104:2193-2198(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-55, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  12. "Tetratricopeptide repeat proteins Tom70 and Tom71 mediate yeast mitochondrial morphogenesis."
    Kondo-Okamoto N., Shaw J.M., Okamoto K.
    EMBO Rep. 9:63-69(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH MBF1.
  13. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
    Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
    Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-55, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  14. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
    Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
    Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-55, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiTOM71_YEAST
AccessioniPrimary (citable) accession number: P38825
Secondary accession number(s): D3DL67
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: February 1, 1995
Last modified: July 6, 2016
This is version 140 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 4110 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome VIII
    Yeast (Saccharomyces cerevisiae) chromosome VIII: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.