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Protein

E3 ubiquitin-protein ligase DMA1

Gene

DMA1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

E3 ubiquitin-protein ligase which functions in cell cycle retarding in conjonction with the UBC4 and UBC13/MMS2 complex, 2 E2 ubiquitin conjugationg enzymes (PubMed:18202552). Involved in nutritional control of the cell cycle (PubMed:15319434). Targets the G1 cyclin PCL1 for destruction (PubMed:23264631). Required for proper spindle positioning, likely regulating septin ring deposition at the bud neck (PubMed:15146058).4 Publications

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri327 – 37145RING-typePROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

  • ligase activity Source: UniProtKB-KW
  • ubiquitin-protein transferase activity Source: SGD
  • zinc ion binding Source: InterPro

GO - Biological processi

  • cellular bud neck septin ring organization Source: SGD
  • establishment of mitotic spindle orientation Source: SGD
  • mitotic nuclear division Source: UniProtKB-KW
  • mitotic spindle orientation checkpoint Source: SGD
  • protein autoubiquitination Source: SGD
  • protein localization to bud neck Source: SGD
  • protein ubiquitination Source: SGD
  • protein ubiquitination involved in ubiquitin-dependent protein catabolic process Source: SGD
  • septin ring assembly Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Cell cycle, Cell division, Mitosis, Ubl conjugation pathway

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

BioCyciYEAST:G3O-31157-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
E3 ubiquitin-protein ligase DMA11 Publication (EC:6.3.2.-1 Publication)
Alternative name(s):
Checkpoint forkhead associated with RING domains-containing protein 11 Publication
Defective in mitotic arrest protein 11 Publication
Gene namesi
Name:DMA11 Publication
Synonyms:CHF11 Publication
Ordered Locus Names:YHR115CImported
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome VIII

Organism-specific databases

EuPathDBiFungiDB:YHR115C.
SGDiS000001157. DMA1.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi192 – 1921G → E: Decreases the interaction with CDC123. 1 Publication
Mutagenesisi220 – 2201S → A: Decreases the interaction with CDC123, when associated with L-223. 1 Publication
Mutagenesisi223 – 2231H → L: Decreases the interaction with CDC123, when associated with A-220. 1 Publication
Mutagenesisi345 – 3451C → S: Decreases the interaction with CDC123, when associated with A-350. 1 Publication
Mutagenesisi350 – 3501H → A: Decreases the interaction with CDC123, when associated with S-345. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 416416E3 ubiquitin-protein ligase DMA1PRO_0000056338Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Cross-linki150 – 150Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)1 Publication
Cross-linki204 – 204Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)1 Publication
Cross-linki217 – 217Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)1 Publication
Cross-linki237 – 237Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)1 Publication
Cross-linki240 – 240Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)1 Publication
Cross-linki260 – 260Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)1 Publication
Cross-linki300 – 300Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)1 Publication
Cross-linki306 – 306Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)1 Publication
Cross-linki313 – 313Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)1 Publication
Cross-linki317 – 317Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)1 Publication

Post-translational modificationi

UBC4-dependent autoubiquitination occurs at Lys-150, Lys-204, Lys-217, Lys-237, Lys-240, Lys-260, Lys-300, Lys-306, Lys-313 and Lys-317. UBC4-dependent autoubiquitination is responsible for DMA2 turnover. UBC13/MMS2-dependent autoubiquitination occurs at Lys-237 and Lys-306. Lys-204 and Lys-306 are also ubiquitinated in trans by DMA2 E3 ligase in association with UBC4.1 Publication

Keywords - PTMi

Isopeptide bond, Ubl conjugation

Proteomic databases

MaxQBiP38823.

Expressioni

Inductioni

Protein levels are regulated by nutrient status, being high under good nutrient conditions.1 Publication

Interactioni

Subunit structurei

Interacts with CDC123 (PubMed:15319434). Interacts with PCL1 (PubMed:23264631).2 Publications

Protein-protein interaction databases

BioGridi36548. 70 interactions.
DIPiDIP-1884N.
IntActiP38823. 22 interactions.
MINTiMINT-388035.

Structurei

3D structure databases

ProteinModelPortaliP38823.
SMRiP38823. Positions 322-374.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini189 – 25264FHAPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the DMA1 family.Curated
Contains 1 FHA domain.PROSITE-ProRule annotation
Contains 1 RING-type zinc finger.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri327 – 37145RING-typePROSITE-ProRule annotationAdd
BLAST

Keywords - Domaini

Zinc-finger

Phylogenomic databases

GeneTreeiENSGT00510000054808.
HOGENOMiHOG000248264.
InParanoidiP38823.
OMAiPERIDQS.
OrthoDBiEOG092C3UWR.

Family and domain databases

Gene3Di2.60.200.20. 1 hit.
3.30.40.10. 1 hit.
InterProiIPR000253. FHA_dom.
IPR008984. SMAD_FHA_domain.
IPR001841. Znf_RING.
IPR013083. Znf_RING/FYVE/PHD.
[Graphical view]
PfamiPF00498. FHA. 1 hit.
PF17123. zf-RING_11. 1 hit.
[Graphical view]
SMARTiSM00240. FHA. 1 hit.
[Graphical view]
SUPFAMiSSF49879. SSF49879. 1 hit.
PROSITEiPS50006. FHA_DOMAIN. 1 hit.
PS50089. ZF_RING_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P38823-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSTNTVPSSP PNQTPPAASG IATSHDHTKF NNPIRLPISI SLTINDTPNN
60 70 80 90 100
NSNNNSVSNG LGILPSRTAT SLVVANNGSA NGNVGATAAA AATVETNTAP
110 120 130 140 150
AVNTTKSIRH FIYPPNQVNQ TEFSLDIHLP PNTSLPERID QSTLKRRMDK
160 170 180 190 200
HGLFSIRLTP FIDTSSTSVA NQGLFFDPII RTAGAGSQII IGRYTERVRE
210 220 230 240 250
AISKIPDQYH PVVFKSKVIS RTHGCFKVDD QGNWFLKDVK SSSGTFLNHQ
260 270 280 290 300
RLSSASTTSK DYLLHDGDII QLGMDFRGGT EEIYRCVKMK IELNKSWKLK
310 320 330 340 350
ANAFNKEALS RIKNLQKLTT GLEQEDCSIC LNKIKPCQAI FISPCAHSWH
360 370 380 390 400
FHCVRRLVIM NYPQFMCPNC RTNCDLETTL ESESESEFEN EDEDEPDIEM
410
DIDMEINNNL GVRLVD
Length:416
Mass (Da):46,098
Last modified:February 1, 1995 - v1
Checksum:i4344AB155C83CBF7
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BK005578 Genomic DNA. Translation: DAA05593.1.
U00059 Genomic DNA. Translation: AAB68865.1.
BK006934 Genomic DNA. Translation: DAA06809.1.
PIRiS48957.
RefSeqiNP_011983.1. NM_001179245.1.

Genome annotation databases

EnsemblFungiiYHR115C; YHR115C; YHR115C.
GeneIDi856515.
KEGGisce:YHR115C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BK005578 Genomic DNA. Translation: DAA05593.1.
U00059 Genomic DNA. Translation: AAB68865.1.
BK006934 Genomic DNA. Translation: DAA06809.1.
PIRiS48957.
RefSeqiNP_011983.1. NM_001179245.1.

3D structure databases

ProteinModelPortaliP38823.
SMRiP38823. Positions 322-374.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi36548. 70 interactions.
DIPiDIP-1884N.
IntActiP38823. 22 interactions.
MINTiMINT-388035.

Proteomic databases

MaxQBiP38823.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYHR115C; YHR115C; YHR115C.
GeneIDi856515.
KEGGisce:YHR115C.

Organism-specific databases

EuPathDBiFungiDB:YHR115C.
SGDiS000001157. DMA1.

Phylogenomic databases

GeneTreeiENSGT00510000054808.
HOGENOMiHOG000248264.
InParanoidiP38823.
OMAiPERIDQS.
OrthoDBiEOG092C3UWR.

Enzyme and pathway databases

BioCyciYEAST:G3O-31157-MONOMER.

Miscellaneous databases

PROiP38823.

Family and domain databases

Gene3Di2.60.200.20. 1 hit.
3.30.40.10. 1 hit.
InterProiIPR000253. FHA_dom.
IPR008984. SMAD_FHA_domain.
IPR001841. Znf_RING.
IPR013083. Znf_RING/FYVE/PHD.
[Graphical view]
PfamiPF00498. FHA. 1 hit.
PF17123. zf-RING_11. 1 hit.
[Graphical view]
SMARTiSM00240. FHA. 1 hit.
[Graphical view]
SUPFAMiSSF49879. SSF49879. 1 hit.
PROSITEiPS50006. FHA_DOMAIN. 1 hit.
PS50089. ZF_RING_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiDMA1_YEAST
AccessioniPrimary (citable) accession number: P38823
Secondary accession number(s): D3DL65, Q66GT1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: February 1, 1995
Last modified: September 7, 2016
This is version 134 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 2790 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome VIII
    Yeast (Saccharomyces cerevisiae) chromosome VIII: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.