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P38822

- BZZ1_YEAST

UniProt

P38822 - BZZ1_YEAST

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Protein

Protein BZZ1

Gene

BZZ1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Plays a role in endocytosis and trafficking to the vacuole. Functions with type I myosins to restore polarity of the actin cytoskeleton after NaCl stress.2 Publications

GO - Molecular functioni

  1. enzyme activator activity Source: SGD

GO - Biological processi

  1. actin filament organization Source: SGD
  2. actin nucleation Source: SGD
  3. endocytosis Source: SGD
  4. positive regulation of catalytic activity Source: GOC
  5. response to salt stress Source: SGD
Complete GO annotation...

Keywords - Biological processi

Endocytosis

Enzyme and pathway databases

BioCyciYEAST:G3O-31156-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Protein BZZ1
Alternative name(s):
LAS17-binding protein 7
Gene namesi
Name:BZZ1
Synonyms:LSB7
Ordered Locus Names:YHR114W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome VIII

Organism-specific databases

CYGDiYHR114w.
SGDiS000001156. BZZ1.

Subcellular locationi

Cytoplasmcytoskeletonactin patch 1 Publication
Note: localizes in cortical actin patches in a LAS17-dependent manner.

GO - Cellular componenti

  1. actin cortical patch Source: SGD
  2. plasma membrane Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 633633Protein BZZ1PRO_0000065033Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei327 – 3271Phosphoserine1 Publication
Modified residuei463 – 4631Phosphoserine2 Publications
Modified residuei472 – 4721Phosphoserine2 Publications
Modified residuei476 – 4761Phosphoserine2 Publications

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP38822.
PaxDbiP38822.

Expressioni

Gene expression databases

GenevestigatoriP38822.

Interactioni

Subunit structurei

Interacts with LAS17 and MYO5.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
AIM21P405637EBI-3889,EBI-25376
AIM3P382662EBI-3889,EBI-21584
APP1P5393314EBI-3889,EBI-28798
LAS17Q1244614EBI-3889,EBI-10022
LDB17Q123423EBI-3889,EBI-38872
MYO5Q044395EBI-3889,EBI-11687
UBP7P404533EBI-3889,EBI-19857
YPL277CQ089892EBI-3889,EBI-38031

Protein-protein interaction databases

BioGridi36547. 126 interactions.
DIPiDIP-2143N.
IntActiP38822. 101 interactions.
MINTiMINT-474480.
STRINGi4932.YHR114W.

Structurei

Secondary structure

1
633
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi520 – 5245
Beta strandi527 – 53610
Turni537 – 5404
Beta strandi541 – 5466
Helixi547 – 5493
Beta strandi582 – 5843
Beta strandi603 – 6086
Beta strandi612 – 6209
Beta strandi623 – 6286
Helixi629 – 6313

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1ZUUX-ray0.97A498-552[»]
2A28X-ray1.07A/B/C/D582-633[»]
ProteinModelPortaliP38822.
SMRiP38822. Positions 14-243, 497-552, 582-633.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP38822.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini5 – 6864FCHPROSITE-ProRule annotationAdd
BLAST
Domaini493 – 55563SH3 1PROSITE-ProRule annotationAdd
BLAST
Domaini577 – 63357SH3 2PROSITE-ProRule annotationAdd
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili138 – 21073Sequence AnalysisAdd
BLAST

Sequence similaritiesi

Belongs to the BZZ1 family.Curated
Contains 1 FCH domain.PROSITE-ProRule annotation
Contains 2 SH3 domains.PROSITE-ProRule annotation

Keywords - Domaini

Coiled coil, Repeat, SH3 domain

Phylogenomic databases

eggNOGiNOG323796.
HOGENOMiHOG000185865.
InParanoidiP38822.
OMAiNIYHELE.
OrthoDBiEOG78SQSK.

Family and domain databases

InterProiIPR001060. FCH_dom.
IPR001452. SH3_domain.
[Graphical view]
PfamiPF00611. FCH. 1 hit.
PF00018. SH3_1. 1 hit.
PF14604. SH3_9. 1 hit.
[Graphical view]
SMARTiSM00055. FCH. 1 hit.
SM00326. SH3. 2 hits.
[Graphical view]
SUPFAMiSSF50044. SSF50044. 2 hits.
PROSITEiPS50133. FCH. 1 hit.
PS50002. SH3. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P38822 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MSADLSIGNE IKDSFKETHK WVQNNLKWLK DIEQFYRERA KLEKDYSERL
60 70 80 90 100
SRLSAEYFNK KSSTSVPISV GDTPTTTPGS IEAAGVVAWN EILSQTDMIS
110 120 130 140 150
KDHDQLSTDF ENHVANQLSG LFTKLDMTLS KINGFNNDMV NKKDNIYHEL
160 170 180 190 200
EKAKKDYDEA CSTMEMARNR YTKASNDRNK KKLDEKEMEM NKCKNEYLIK
210 220 230 240 250
INQANRTKDK YYFQDVPEVL DLLQDVNEAK TLFLNDLWLK AASVENDLGA
260 270 280 290 300
NVSKRLQAAN SVVKQNKPSL NTAIFIKHNL KNWKEPQDFV YKPSPVWHDD
310 320 330 340 350
EKFAVPSSLE VEDLRIKLAK AENDYNSLQD KTQNELSKLS TLNKIKHEMK
360 370 380 390 400
TNEDNINATK FYDTLKEYLN VVSPFTSHET LKLQAEVQIE SIQNNVPEEY
410 420 430 440 450
DLSTDNIDLS KTKKKSGIFS KFKHNILNVD SKPSSGGSTG NGNGGPLHIT
460 470 480 490 500
SLFNTSRRTR LGSAPNNAGE DSDNNSIRTT STNNTKKTTQ NSSDDGKNKV
510 520 530 540 550
LYAYVQKDDD EITITPGDKI SLVARDTGSG WTKINNDTTG ETGLVPTTYI
560 570 580 590 600
RISSAATVKA NDRGPAPEVP PPRRSTLPVR TMEAIYAYEA QGDDEISIDP
610 620 630
GDIITVIRGD DGSGWTYGEC DGLKGLFPTS YCK
Length:633
Mass (Da):71,171
Last modified:February 1, 1995 - v1
Checksum:i5C73DACC69611B41
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti196 – 1961E → G in AAS56434. (PubMed:17322287)Curated

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U00059 Genomic DNA. Translation: AAB68850.1.
AY558108 Genomic DNA. Translation: AAS56434.1.
BK006934 Genomic DNA. Translation: DAA06808.1.
PIRiS48956.
RefSeqiNP_011982.1. NM_001179244.1.

Genome annotation databases

EnsemblFungiiYHR114W; YHR114W; YHR114W.
GeneIDi856514.
KEGGisce:YHR114W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U00059 Genomic DNA. Translation: AAB68850.1 .
AY558108 Genomic DNA. Translation: AAS56434.1 .
BK006934 Genomic DNA. Translation: DAA06808.1 .
PIRi S48956.
RefSeqi NP_011982.1. NM_001179244.1.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1ZUU X-ray 0.97 A 498-552 [» ]
2A28 X-ray 1.07 A/B/C/D 582-633 [» ]
ProteinModelPortali P38822.
SMRi P38822. Positions 14-243, 497-552, 582-633.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 36547. 126 interactions.
DIPi DIP-2143N.
IntActi P38822. 101 interactions.
MINTi MINT-474480.
STRINGi 4932.YHR114W.

Proteomic databases

MaxQBi P38822.
PaxDbi P38822.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YHR114W ; YHR114W ; YHR114W .
GeneIDi 856514.
KEGGi sce:YHR114W.

Organism-specific databases

CYGDi YHR114w.
SGDi S000001156. BZZ1.

Phylogenomic databases

eggNOGi NOG323796.
HOGENOMi HOG000185865.
InParanoidi P38822.
OMAi NIYHELE.
OrthoDBi EOG78SQSK.

Enzyme and pathway databases

BioCyci YEAST:G3O-31156-MONOMER.

Miscellaneous databases

EvolutionaryTracei P38822.
NextBioi 982260.
PROi P38822.

Gene expression databases

Genevestigatori P38822.

Family and domain databases

InterProi IPR001060. FCH_dom.
IPR001452. SH3_domain.
[Graphical view ]
Pfami PF00611. FCH. 1 hit.
PF00018. SH3_1. 1 hit.
PF14604. SH3_9. 1 hit.
[Graphical view ]
SMARTi SM00055. FCH. 1 hit.
SM00326. SH3. 2 hits.
[Graphical view ]
SUPFAMi SSF50044. SSF50044. 2 hits.
PROSITEi PS50133. FCH. 1 hit.
PS50002. SH3. 2 hits.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  2. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  3. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  4. "Saccharomyces cerevisiae Bzz1p is implicated with type I myosins in actin patch polarization and is able to recruit actin-polymerizing machinery in vitro."
    Soulard A., Lechler T., Spiridonov V., Shevchenko A., Shevchenko A., Li R., Winsor B.
    Mol. Cell. Biol. 22:7889-7906(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION, INTERACTION WITH LAS17 AND MYO5.
  5. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  6. "The WASP/Las17p-interacting protein Bzz1p functions with Myo5p in an early stage of endocytosis."
    Soulard A., Friant S., Fitterer C., Orange C., Kaneva G., Mirey G., Winsor B.
    Protoplasma 226:89-101(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  7. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
    Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
    Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-327; SER-463; SER-472 AND SER-476, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  8. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
    Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
    Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-463; SER-472 AND SER-476, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  9. "Structural genomics of yeast SH3 domains."
    Kursula P., Kursula I., Lehmann F., Zou P., Song Y.H., Wilmanns M.
    Submitted (JUN-2005) to the PDB data bank
    Cited for: X-RAY CRYSTALLOGRAPHY (0.97 ANGSTROMS) OF 498-633.

Entry informationi

Entry nameiBZZ1_YEAST
AccessioniPrimary (citable) accession number: P38822
Secondary accession number(s): D3DL64, E9P8V2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: February 1, 1995
Last modified: October 29, 2014
This is version 123 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 5440 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome VIII
    Yeast (Saccharomyces cerevisiae) chromosome VIII: entries and gene names

External Data

Dasty 3