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P38822

- BZZ1_YEAST

UniProt

P38822 - BZZ1_YEAST

Protein

Protein BZZ1

Gene

BZZ1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 122 (01 Oct 2014)
      Sequence version 1 (01 Feb 1995)
      Previous versions | rss
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    Functioni

    Plays a role in endocytosis and trafficking to the vacuole. Functions with type I myosins to restore polarity of the actin cytoskeleton after NaCl stress.2 Publications

    GO - Molecular functioni

    1. enzyme activator activity Source: SGD
    2. protein binding Source: IntAct

    GO - Biological processi

    1. actin filament organization Source: SGD
    2. actin nucleation Source: SGD
    3. endocytosis Source: SGD
    4. positive regulation of catalytic activity Source: GOC
    5. response to salt stress Source: SGD

    Keywords - Biological processi

    Endocytosis

    Enzyme and pathway databases

    BioCyciYEAST:G3O-31156-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Protein BZZ1
    Alternative name(s):
    LAS17-binding protein 7
    Gene namesi
    Name:BZZ1
    Synonyms:LSB7
    Ordered Locus Names:YHR114W
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome VIII

    Organism-specific databases

    CYGDiYHR114w.
    SGDiS000001156. BZZ1.

    Subcellular locationi

    Cytoplasmcytoskeletonactin patch 1 Publication
    Note: localizes in cortical actin patches in a LAS17-dependent manner.

    GO - Cellular componenti

    1. actin cortical patch Source: SGD
    2. plasma membrane Source: SGD

    Keywords - Cellular componenti

    Cytoplasm, Cytoskeleton

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 633633Protein BZZ1PRO_0000065033Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei327 – 3271Phosphoserine1 Publication
    Modified residuei463 – 4631Phosphoserine2 Publications
    Modified residuei472 – 4721Phosphoserine2 Publications
    Modified residuei476 – 4761Phosphoserine2 Publications

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    MaxQBiP38822.
    PaxDbiP38822.

    Expressioni

    Gene expression databases

    GenevestigatoriP38822.

    Interactioni

    Subunit structurei

    Interacts with LAS17 and MYO5.1 Publication

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    AIM21P405637EBI-3889,EBI-25376
    AIM3P382662EBI-3889,EBI-21584
    APP1P5393314EBI-3889,EBI-28798
    LAS17Q1244614EBI-3889,EBI-10022
    LDB17Q123423EBI-3889,EBI-38872
    MYO5Q044395EBI-3889,EBI-11687
    UBP7P404533EBI-3889,EBI-19857
    YPL277CQ089892EBI-3889,EBI-38031

    Protein-protein interaction databases

    BioGridi36547. 124 interactions.
    DIPiDIP-2143N.
    IntActiP38822. 101 interactions.
    MINTiMINT-474480.
    STRINGi4932.YHR114W.

    Structurei

    Secondary structure

    1
    633
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi520 – 5245
    Beta strandi527 – 53610
    Turni537 – 5404
    Beta strandi541 – 5466
    Helixi547 – 5493
    Beta strandi582 – 5843
    Beta strandi603 – 6086
    Beta strandi612 – 6209
    Beta strandi623 – 6286
    Helixi629 – 6313

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1ZUUX-ray0.97A498-552[»]
    2A28X-ray1.07A/B/C/D582-633[»]
    ProteinModelPortaliP38822.
    SMRiP38822. Positions 14-243, 497-552, 582-633.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP38822.

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini5 – 6864FCHPROSITE-ProRule annotationAdd
    BLAST
    Domaini493 – 55563SH3 1PROSITE-ProRule annotationAdd
    BLAST
    Domaini577 – 63357SH3 2PROSITE-ProRule annotationAdd
    BLAST

    Coiled coil

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Coiled coili138 – 21073Sequence AnalysisAdd
    BLAST

    Sequence similaritiesi

    Belongs to the BZZ1 family.Curated
    Contains 1 FCH domain.PROSITE-ProRule annotation
    Contains 2 SH3 domains.PROSITE-ProRule annotation

    Keywords - Domaini

    Coiled coil, Repeat, SH3 domain

    Phylogenomic databases

    eggNOGiNOG323796.
    HOGENOMiHOG000185865.
    OMAiNIYHELE.
    OrthoDBiEOG78SQSK.

    Family and domain databases

    InterProiIPR001060. FCH_dom.
    IPR001452. SH3_domain.
    [Graphical view]
    PfamiPF00611. FCH. 1 hit.
    PF00018. SH3_1. 1 hit.
    PF14604. SH3_9. 1 hit.
    [Graphical view]
    SMARTiSM00055. FCH. 1 hit.
    SM00326. SH3. 2 hits.
    [Graphical view]
    SUPFAMiSSF50044. SSF50044. 2 hits.
    PROSITEiPS50133. FCH. 1 hit.
    PS50002. SH3. 2 hits.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P38822-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSADLSIGNE IKDSFKETHK WVQNNLKWLK DIEQFYRERA KLEKDYSERL    50
    SRLSAEYFNK KSSTSVPISV GDTPTTTPGS IEAAGVVAWN EILSQTDMIS 100
    KDHDQLSTDF ENHVANQLSG LFTKLDMTLS KINGFNNDMV NKKDNIYHEL 150
    EKAKKDYDEA CSTMEMARNR YTKASNDRNK KKLDEKEMEM NKCKNEYLIK 200
    INQANRTKDK YYFQDVPEVL DLLQDVNEAK TLFLNDLWLK AASVENDLGA 250
    NVSKRLQAAN SVVKQNKPSL NTAIFIKHNL KNWKEPQDFV YKPSPVWHDD 300
    EKFAVPSSLE VEDLRIKLAK AENDYNSLQD KTQNELSKLS TLNKIKHEMK 350
    TNEDNINATK FYDTLKEYLN VVSPFTSHET LKLQAEVQIE SIQNNVPEEY 400
    DLSTDNIDLS KTKKKSGIFS KFKHNILNVD SKPSSGGSTG NGNGGPLHIT 450
    SLFNTSRRTR LGSAPNNAGE DSDNNSIRTT STNNTKKTTQ NSSDDGKNKV 500
    LYAYVQKDDD EITITPGDKI SLVARDTGSG WTKINNDTTG ETGLVPTTYI 550
    RISSAATVKA NDRGPAPEVP PPRRSTLPVR TMEAIYAYEA QGDDEISIDP 600
    GDIITVIRGD DGSGWTYGEC DGLKGLFPTS YCK 633
    Length:633
    Mass (Da):71,171
    Last modified:February 1, 1995 - v1
    Checksum:i5C73DACC69611B41
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti196 – 1961E → G in AAS56434. (PubMed:17322287)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U00059 Genomic DNA. Translation: AAB68850.1.
    AY558108 Genomic DNA. Translation: AAS56434.1.
    BK006934 Genomic DNA. Translation: DAA06808.1.
    PIRiS48956.
    RefSeqiNP_011982.1. NM_001179244.1.

    Genome annotation databases

    EnsemblFungiiYHR114W; YHR114W; YHR114W.
    GeneIDi856514.
    KEGGisce:YHR114W.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U00059 Genomic DNA. Translation: AAB68850.1 .
    AY558108 Genomic DNA. Translation: AAS56434.1 .
    BK006934 Genomic DNA. Translation: DAA06808.1 .
    PIRi S48956.
    RefSeqi NP_011982.1. NM_001179244.1.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1ZUU X-ray 0.97 A 498-552 [» ]
    2A28 X-ray 1.07 A/B/C/D 582-633 [» ]
    ProteinModelPortali P38822.
    SMRi P38822. Positions 14-243, 497-552, 582-633.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 36547. 124 interactions.
    DIPi DIP-2143N.
    IntActi P38822. 101 interactions.
    MINTi MINT-474480.
    STRINGi 4932.YHR114W.

    Proteomic databases

    MaxQBi P38822.
    PaxDbi P38822.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YHR114W ; YHR114W ; YHR114W .
    GeneIDi 856514.
    KEGGi sce:YHR114W.

    Organism-specific databases

    CYGDi YHR114w.
    SGDi S000001156. BZZ1.

    Phylogenomic databases

    eggNOGi NOG323796.
    HOGENOMi HOG000185865.
    OMAi NIYHELE.
    OrthoDBi EOG78SQSK.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-31156-MONOMER.

    Miscellaneous databases

    EvolutionaryTracei P38822.
    NextBioi 982260.
    PROi P38822.

    Gene expression databases

    Genevestigatori P38822.

    Family and domain databases

    InterProi IPR001060. FCH_dom.
    IPR001452. SH3_domain.
    [Graphical view ]
    Pfami PF00611. FCH. 1 hit.
    PF00018. SH3_1. 1 hit.
    PF14604. SH3_9. 1 hit.
    [Graphical view ]
    SMARTi SM00055. FCH. 1 hit.
    SM00326. SH3. 2 hits.
    [Graphical view ]
    SUPFAMi SSF50044. SSF50044. 2 hits.
    PROSITEi PS50133. FCH. 1 hit.
    PS50002. SH3. 2 hits.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    2. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    3. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    4. "Saccharomyces cerevisiae Bzz1p is implicated with type I myosins in actin patch polarization and is able to recruit actin-polymerizing machinery in vitro."
      Soulard A., Lechler T., Spiridonov V., Shevchenko A., Shevchenko A., Li R., Winsor B.
      Mol. Cell. Biol. 22:7889-7906(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, SUBCELLULAR LOCATION, INTERACTION WITH LAS17 AND MYO5.
    5. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
    6. "The WASP/Las17p-interacting protein Bzz1p functions with Myo5p in an early stage of endocytosis."
      Soulard A., Friant S., Fitterer C., Orange C., Kaneva G., Mirey G., Winsor B.
      Protoplasma 226:89-101(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    7. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
      Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
      Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-327; SER-463; SER-472 AND SER-476, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    8. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
      Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
      Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-463; SER-472 AND SER-476, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    9. "Structural genomics of yeast SH3 domains."
      Kursula P., Kursula I., Lehmann F., Zou P., Song Y.H., Wilmanns M.
      Submitted (JUN-2005) to the PDB data bank
      Cited for: X-RAY CRYSTALLOGRAPHY (0.97 ANGSTROMS) OF 498-633.

    Entry informationi

    Entry nameiBZZ1_YEAST
    AccessioniPrimary (citable) accession number: P38822
    Secondary accession number(s): D3DL64, E9P8V2
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 1, 1995
    Last sequence update: February 1, 1995
    Last modified: October 1, 2014
    This is version 122 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 5440 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families
    3. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    4. Yeast chromosome VIII
      Yeast (Saccharomyces cerevisiae) chromosome VIII: entries and gene names

    External Data

    Dasty 3