Protein BZZ1 - Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

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P38822 (BZZ1_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 111. History...

Clusters with 100%, 90%, 50% identity |

Documents (4) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Protein BZZ1

Alternative name(s):
LAS17-binding protein 7

Gene names

Name:	BZZ1
Synonyms:	LSB7
Ordered Locus Names:	YHR114W

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces ›

Protein attributes

Sequence length	633 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Plays a role in endocytosis and trafficking to the vacuole. Functions with type I myosins to restore polarity of the actin cytoskeleton after NaCl stress. Ref.4 Ref.6
Subunit structure	Interacts with LAS17 and MYO5. Ref.4
Subcellular location	Cytoplasm › cytoskeleton › actin patch. Note: localizes in cortical actin patches in a LAS17-dependent manner. Ref.4
Miscellaneous	Present with 5440 molecules/cell in log phase SD medium.
Sequence similarities	Belongs to the BZZ1 family. Contains 1 FCH domain. Contains 2 SH3 domains.

Ontologies

Keywords
Biological process	Endocytosis
Cellular component	Cytoplasm Cytoskeleton
Domain	Coiled coil Repeat SH3 domain
PTM	Phosphoprotein
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	actin filament organization Inferred from physical interaction Ref.4. Source: SGD endocytosis Inferred from genetic interaction Ref.6. Source: SGD response to salt stress Inferred from physical interaction Ref.4. Source: SGD
Cellular_component	actin cortical patch Inferred from direct assay Ref.4. Source: SGD mating projection tip Inferred from direct assay PubMed 19053807. Source: SGD
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct
APP1	P53933	7	EBI-3889,EBI-28798
LAS17	Q12446	23	EBI-3889,EBI-10022
MYO5	Q04439	3	EBI-3889,EBI-11687

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 633

633

Protein BZZ1

PRO_0000065033

Regions

Domain

5 – 68

FCH

Domain

493 – 555

SH3 1

Domain

577 – 633

SH3 2

Coiled coil

138 – 210

Potential

Amino acid modifications

Modified residue

327

Phosphoserine Ref.8

Modified residue

463

Phosphoserine Ref.8

Modified residue

472

Phosphoserine Ref.8

Modified residue

476

Phosphoserine Ref.8

Modified residue

576

Phosphothreonine Ref.7

Experimental info

Sequence conflict

196

E → G in AAS56434. Ref.3

Secondary structure

633

Helix Strand Turn

Details...

Sequences

Sequence

Length

Mass (Da)

Tools

P38822 [UniParc].

Last modified February 1, 1995. Version 1.
Checksum: 5C73DACC69611B41
Show »

FASTA

633

71,171

        10         20         30         40         50         60 
MSADLSIGNE IKDSFKETHK WVQNNLKWLK DIEQFYRERA KLEKDYSERL SRLSAEYFNK 

        70         80         90        100        110        120 
KSSTSVPISV GDTPTTTPGS IEAAGVVAWN EILSQTDMIS KDHDQLSTDF ENHVANQLSG 

       130        140        150        160        170        180 
LFTKLDMTLS KINGFNNDMV NKKDNIYHEL EKAKKDYDEA CSTMEMARNR YTKASNDRNK 

       190        200        210        220        230        240 
KKLDEKEMEM NKCKNEYLIK INQANRTKDK YYFQDVPEVL DLLQDVNEAK TLFLNDLWLK 

       250        260        270        280        290        300 
AASVENDLGA NVSKRLQAAN SVVKQNKPSL NTAIFIKHNL KNWKEPQDFV YKPSPVWHDD 

       310        320        330        340        350        360 
EKFAVPSSLE VEDLRIKLAK AENDYNSLQD KTQNELSKLS TLNKIKHEMK TNEDNINATK 

       370        380        390        400        410        420 
FYDTLKEYLN VVSPFTSHET LKLQAEVQIE SIQNNVPEEY DLSTDNIDLS KTKKKSGIFS 

       430        440        450        460        470        480 
KFKHNILNVD SKPSSGGSTG NGNGGPLHIT SLFNTSRRTR LGSAPNNAGE DSDNNSIRTT 

       490        500        510        520        530        540 
STNNTKKTTQ NSSDDGKNKV LYAYVQKDDD EITITPGDKI SLVARDTGSG WTKINNDTTG 

       550        560        570        580        590        600 
ETGLVPTTYI RISSAATVKA NDRGPAPEVP PPRRSTLPVR TMEAIYAYEA QGDDEISIDP 

       610        620        630 
GDIITVIRGD DGSGWTYGEC DGLKGLFPTS YCK

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Complete nucleotide sequence of Saccharomyces cerevisiae chromosome VIII." Johnston M., Andrews S., Brinkman R., Cooper J., Ding H., Dover J., Du Z., Favello A., Fulton L., Gattung S., Geisel C., Kirsten J., Kucaba T., Hillier L.W., Jier M., Johnston L., Langston Y., Latreille P. Vaudin M. Science 265:2077-2082(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[2]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[3]	"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae." Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. LaBaer J. Genome Res. 17:536-543(2007) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[4]	"Saccharomyces cerevisiae Bzz1p is implicated with type I myosins in actin patch polarization and is able to recruit actin-polymerizing machinery in vitro." Soulard A., Lechler T., Spiridonov V., Shevchenko A., Shevchenko A., Li R., Winsor B. Mol. Cell. Biol. 22:7889-7906(2002) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION, INTERACTION WITH LAS17 AND MYO5.
[5]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[6]	"The WASP/Las17p-interacting protein Bzz1p functions with Myo5p in an early stage of endocytosis." Soulard A., Friant S., Fitterer C., Orange C., Kaneva G., Mirey G., Winsor B. Protoplasma 226:89-101(2005) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
[7]	"Proteome-wide identification of in vivo targets of DNA damage checkpoint kinases." Smolka M.B., Albuquerque C.P., Chen S.H., Zhou H. Proc. Natl. Acad. Sci. U.S.A. 104:10364-10369(2007) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-576, MASS SPECTROMETRY.
[8]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-327; SER-463; SER-472 AND SER-476, MASS SPECTROMETRY.
[9]	"Structural genomics of yeast SH3 domains." Kursula P., Kursula I., Lehmann F., Zou P., Song Y.H., Wilmanns M. Submitted (JUN-2005) to the PDB data bank Cited for: X-RAY CRYSTALLOGRAPHY (0.97 ANGSTROMS) OF 498-633.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

U00059 Genomic DNA. Translation: AAB68850.1.
AY558108 Genomic DNA. Translation: AAS56434.1.
BK006934 Genomic DNA. Translation: DAA06808.1.

PIR

S48956.

RefSeq

NP_011982.1. NM_001179244.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1ZUU	X-ray	0.97	A	498-552	[»]
2A28	X-ray	1.07	A/B/C/D	582-633	[»]

ProteinModelPortal

P38822.

SMR

P38822. Positions 14-243, 497-552, 582-633.

ModBase

Search...

Protein-protein interaction databases

DIP

DIP-2143N.

IntAct

P38822. 47 interactions.

MINT

MINT-474480.

STRING

4932.YHR114W.

Proteomic databases

PaxDb

P38822.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

EnsemblFungi

YHR114W; YHR114W; YHR114W.

GeneID

856514.

KEGG

sce:YHR114W.

Organism-specific databases

CYGD

YHR114w.

SGD

S000001156. BZZ1.

Phylogenomic databases

eggNOG

NOG323796.

GeneTree

ENSGT00510000046403.

HOGENOM

HOG000185865.

OMA

SVPANYV.

OrthoDB

EOG4Z924N.

Gene expression databases

Genevestigator

P38822.

GermOnline

YHR114W. Saccharomyces cerevisiae.

Family and domain databases

InterPro

IPR001060. FCH_dom.
IPR001452. SH3_domain.
[Graphical view]

Pfam

PF00611. FCH. 1 hit.
PF00018. SH3_1. 2 hits.
[Graphical view]

SMART

SM00055. FCH. 1 hit.
SM00326. SH3. 2 hits.
[Graphical view]

SUPFAM

SSF50044. SH3. 2 hits.

PROSITE

PS50133. FCH. 1 hit.
PS50002. SH3. 2 hits.
[Graphical view]

ProtoNet

Search...

Other

EvolutionaryTrace

P38822.

NextBio

982260.

Entry information

Entry name

BZZ1_YEAST

Accession

Primary (citable) accession number: P38822
Secondary accession number(s): D3DL64, E9P8V2

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1995
Last sequence update:	February 1, 1995
Last modified:	May 1, 2013
This is version 111 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program

Relevant documents

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

Yeast chromosome VIII

Yeast (Saccharomyces cerevisiae) chromosome VIII: entries and gene names

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Binary interactions

With

Entry

#Exp.

IntAct

Notes

Sequence annotation (Features)

Molecule processing

Regions

Amino acid modifications

Experimental info

Secondary structure

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents