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Protein

ADP-ribosylation factor-binding protein GGA2

Gene

GGA2

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

May play a role in the regulation of membrane traffic through the trans-Golgi network.2 Publications

GO - Molecular functioni

  • phosphatidylinositol-4-phosphate binding Source: SGD
  • ubiquitin binding Source: SGD

GO - Biological processi

  • Golgi to endosome transport Source: SGD
  • Golgi to vacuole transport Source: SGD
  • protein targeting to vacuole involved in ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway Source: SGD
Complete GO annotation...

Keywords - Biological processi

Protein transport, Transport

Enzyme and pathway databases

BioCyciYEAST:G3O-31151-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
ADP-ribosylation factor-binding protein GGA2
Alternative name(s):
Golgi-localized, gamma ear-containing, ARF-binding protein 2
Gene namesi
Name:GGA2
Ordered Locus Names:YHR108W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome VIII

Organism-specific databases

EuPathDBiFungiDB:YHR108W.
SGDiS000001150. GGA2.

Subcellular locationi

GO - Cellular componenti

  • clathrin adaptor complex Source: InterPro
  • cytosol Source: GOC
  • trans-Golgi network Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Golgi apparatus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi560K → Q: Reduced binding to ENT3. 1 Publication1
Mutagenesisi563W → A: Reduced binding to ENT3 and ENT5. 1 Publication1
Mutagenesisi564K → Q: Reduced binding to ENT3 and ENT5. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002126871 – 585ADP-ribosylation factor-binding protein GGA2Add BLAST585

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Cross-linki180Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)Combined sources
Cross-linki287Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)Combined sources

Keywords - PTMi

Isopeptide bond, Ubl conjugation

Proteomic databases

MaxQBiP38817.
PRIDEiP38817.

Interactioni

Subunit structurei

Binds to ARF1 and ARF2.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
itself2EBI-7569,EBI-7569
MON2P485633EBI-7569,EBI-28333

GO - Molecular functioni

  • ubiquitin binding Source: SGD

Protein-protein interaction databases

BioGridi36541. 85 interactors.
DIPiDIP-2971N.
IntActiP38817. 20 interactors.
MINTiMINT-634509.

Structurei

Secondary structure

1585
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi472 – 477Combined sources6
Beta strandi479 – 489Combined sources11
Beta strandi491 – 493Combined sources3
Beta strandi495 – 503Combined sources9
Beta strandi505 – 507Combined sources3
Beta strandi509 – 517Combined sources9
Beta strandi522 – 526Combined sources5
Beta strandi542 – 550Combined sources9
Beta strandi560 – 569Combined sources10
Beta strandi572 – 581Combined sources10

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3MNMX-ray1.73A/B/C465-585[»]
ProteinModelPortaliP38817.
SMRiP38817.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini33 – 169VHSPROSITE-ProRule annotationAdd BLAST137
Domaini196 – 321GATPROSITE-ProRule annotationAdd BLAST126
Domaini466 – 581GAEPROSITE-ProRule annotationAdd BLAST116

Sequence similaritiesi

Contains 1 GAE domain.PROSITE-ProRule annotation
Contains 1 GAT domain.PROSITE-ProRule annotation
Contains 1 VHS domain.PROSITE-ProRule annotation

Keywords - Domaini

Coiled coil

Phylogenomic databases

GeneTreeiENSGT00860000133703.
HOGENOMiHOG000209783.
InParanoidiP38817.
OMAiMCEEDSE.
OrthoDBiEOG092C1LW8.

Family and domain databases

Gene3Di1.20.58.160. 1 hit.
1.25.40.90. 1 hit.
2.60.40.1230. 1 hit.
InterProiIPR008152. Clathrin_a/b/g-adaptin_app_Ig.
IPR008153. Clathrin_g-adaptin_app.
IPR013041. Coatomer/clathrin_app_Ig-like.
IPR008942. ENTH_VHS.
IPR004152. GAT_dom.
IPR002014. VHS_dom.
[Graphical view]
PfamiPF02883. Alpha_adaptinC2. 1 hit.
PF03127. GAT. 1 hit.
PF00790. VHS. 1 hit.
[Graphical view]
SMARTiSM00809. Alpha_adaptinC2. 1 hit.
SM00288. VHS. 1 hit.
[Graphical view]
SUPFAMiSSF48464. SSF48464. 1 hit.
SSF49348. SSF49348. 1 hit.
PROSITEiPS50180. GAE. 1 hit.
PS50909. GAT. 1 hit.
PS50179. VHS. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P38817-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSHPHSHSIY LSELPVRKPQ ALGNPLLRKI QRACRMSLAE PDLALNLDIA
60 70 80 90 100
DYINEKQGAA PRDAAIALAK LINNRESHVA IFALSLLDVL VKNCGYPFHL
110 120 130 140 150
QISRKEFLNE LVKRFPGHPP LRYSKIQRLI LTAIEEWYQT ICKHSSYKND
160 170 180 190 200
MGYIRDMHRL LKYKGYAFPK ISESDLAVLK PSNQLKTASE IQKEQEIAQA
210 220 230 240 250
AKLEELIRRG KPEDLREANK LMKIMAGFKE DNAVQAKQAI SSELNKLKRK
260 270 280 290 300
ADLLNEMLES PDSQNWDNET TQELHSALKV AQPKFQKIIE EEQEDDALVQ
310 320 330 340 350
DLLKFNDTVN QLLEKFNLLK NGDSNAASQI HPSHVSAPLQ QSSGALTNEI
360 370 380 390 400
NLIDFNDLDE APSQGNNNTN GTGTPAAAET SVNDLLGDLT DLSISNPSTA
410 420 430 440 450
NQASFGLGGD IVLGSSQPAP PVTTTNNSNN TLDLLGLSTP QSPTNSQAVN
460 470 480 490 500
SSGFDLLMGF NPTTGTTTAP ARTLVNQSPN LKIEFEISRE SNSVIRIKSF
510 520 530 540 550
FTNLSSSPIS NLVFLLAVPK SMSLKLQPQS SNFMIGNAKD GISQEGTIEN
560 570 580
APANPSKALK VKWKVNYSVN STQAEETAVF TLPNV
Length:585
Mass (Da):64,347
Last modified:February 1, 1995 - v1
Checksum:i7AC3A317AEE302E2
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00059 Genomic DNA. Translation: AAB68854.1.
BK006934 Genomic DNA. Translation: DAA06802.1.
PIRiS48950.
RefSeqiNP_011976.1. NM_001179238.1.

Genome annotation databases

EnsemblFungiiYHR108W; YHR108W; YHR108W.
GeneIDi856508.
KEGGisce:YHR108W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00059 Genomic DNA. Translation: AAB68854.1.
BK006934 Genomic DNA. Translation: DAA06802.1.
PIRiS48950.
RefSeqiNP_011976.1. NM_001179238.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3MNMX-ray1.73A/B/C465-585[»]
ProteinModelPortaliP38817.
SMRiP38817.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi36541. 85 interactors.
DIPiDIP-2971N.
IntActiP38817. 20 interactors.
MINTiMINT-634509.

Proteomic databases

MaxQBiP38817.
PRIDEiP38817.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYHR108W; YHR108W; YHR108W.
GeneIDi856508.
KEGGisce:YHR108W.

Organism-specific databases

EuPathDBiFungiDB:YHR108W.
SGDiS000001150. GGA2.

Phylogenomic databases

GeneTreeiENSGT00860000133703.
HOGENOMiHOG000209783.
InParanoidiP38817.
OMAiMCEEDSE.
OrthoDBiEOG092C1LW8.

Enzyme and pathway databases

BioCyciYEAST:G3O-31151-MONOMER.

Miscellaneous databases

PROiP38817.

Family and domain databases

Gene3Di1.20.58.160. 1 hit.
1.25.40.90. 1 hit.
2.60.40.1230. 1 hit.
InterProiIPR008152. Clathrin_a/b/g-adaptin_app_Ig.
IPR008153. Clathrin_g-adaptin_app.
IPR013041. Coatomer/clathrin_app_Ig-like.
IPR008942. ENTH_VHS.
IPR004152. GAT_dom.
IPR002014. VHS_dom.
[Graphical view]
PfamiPF02883. Alpha_adaptinC2. 1 hit.
PF03127. GAT. 1 hit.
PF00790. VHS. 1 hit.
[Graphical view]
SMARTiSM00809. Alpha_adaptinC2. 1 hit.
SM00288. VHS. 1 hit.
[Graphical view]
SUPFAMiSSF48464. SSF48464. 1 hit.
SSF49348. SSF49348. 1 hit.
PROSITEiPS50180. GAE. 1 hit.
PS50909. GAT. 1 hit.
PS50179. VHS. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiGGA2_YEAST
AccessioniPrimary (citable) accession number: P38817
Secondary accession number(s): D3DL58
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: February 1, 1995
Last modified: November 30, 2016
This is version 141 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 2270 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome VIII
    Yeast (Saccharomyces cerevisiae) chromosome VIII: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.