Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

P38817 (GGA2_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 118. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
ADP-ribosylation factor-binding protein GGA2
Alternative name(s):
Golgi-localized, gamma ear-containing, ARF-binding protein 2
Gene names
Name:GGA2
Ordered Locus Names:YHR108W
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length585 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

May play a role in the regulation of membrane traffic through the trans-Golgi network. Ref.3 Ref.4

Subunit structure

Binds to ARF1 and ARF2. Ref.4 Ref.5

Subcellular location

Golgi apparatustrans-Golgi network.

Miscellaneous

Present with 2270 molecules/cell in log phase SD medium.

Sequence similarities

Contains 1 GAE domain.

Contains 1 GAT domain.

Contains 1 VHS domain.

Binary interactions

With

Entry

#Exp.

IntAct

Notes

itself2EBI-7569,EBI-7569
MON2P485633EBI-7569,EBI-28333

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 585585ADP-ribosylation factor-binding protein GGA2
PRO_0000212687

Regions

Domain33 – 169137VHS
Domain196 – 321126GAT
Domain466 – 581116GAE

Experimental info

Mutagenesis5601K → Q: Reduced binding to ENT3. Ref.5
Mutagenesis5631W → A: Reduced binding to ENT3 and ENT5. Ref.5
Mutagenesis5641K → Q: Reduced binding to ENT3 and ENT5. Ref.5

Secondary structure

..................... 585
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P38817 [UniParc].

Last modified February 1, 1995. Version 1.
Checksum: 7AC3A317AEE302E2

FASTA58564,347
        10         20         30         40         50         60 
MSHPHSHSIY LSELPVRKPQ ALGNPLLRKI QRACRMSLAE PDLALNLDIA DYINEKQGAA 

        70         80         90        100        110        120 
PRDAAIALAK LINNRESHVA IFALSLLDVL VKNCGYPFHL QISRKEFLNE LVKRFPGHPP 

       130        140        150        160        170        180 
LRYSKIQRLI LTAIEEWYQT ICKHSSYKND MGYIRDMHRL LKYKGYAFPK ISESDLAVLK 

       190        200        210        220        230        240 
PSNQLKTASE IQKEQEIAQA AKLEELIRRG KPEDLREANK LMKIMAGFKE DNAVQAKQAI 

       250        260        270        280        290        300 
SSELNKLKRK ADLLNEMLES PDSQNWDNET TQELHSALKV AQPKFQKIIE EEQEDDALVQ 

       310        320        330        340        350        360 
DLLKFNDTVN QLLEKFNLLK NGDSNAASQI HPSHVSAPLQ QSSGALTNEI NLIDFNDLDE 

       370        380        390        400        410        420 
APSQGNNNTN GTGTPAAAET SVNDLLGDLT DLSISNPSTA NQASFGLGGD IVLGSSQPAP 

       430        440        450        460        470        480 
PVTTTNNSNN TLDLLGLSTP QSPTNSQAVN SSGFDLLMGF NPTTGTTTAP ARTLVNQSPN 

       490        500        510        520        530        540 
LKIEFEISRE SNSVIRIKSF FTNLSSSPIS NLVFLLAVPK SMSLKLQPQS SNFMIGNAKD 

       550        560        570        580 
GISQEGTIEN APANPSKALK VKWKVNYSVN STQAEETAVF TLPNV 

« Hide

References

« Hide 'large scale' references
[1]"Complete nucleotide sequence of Saccharomyces cerevisiae chromosome VIII."
Johnston M., Andrews S., Brinkman R., Cooper J., Ding H., Dover J., Du Z., Favello A., Fulton L., Gattung S., Geisel C., Kirsten J., Kucaba T., Hillier L.W., Jier M., Johnston L., Langston Y., Latreille P. expand/collapse author list , Louis E.J., Macri C., Mardis E., Menezes S., Mouser L., Nhan M., Rifkin L., Riles L., St Peter H., Trevaskis E., Vaughan K., Vignati D., Wilcox L., Wohldman P., Waterston R., Wilson R., Vaudin M.
Science 265:2077-2082(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[2]Saccharomyces Genome Database
Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[3]"A family of proteins with gamma-adaptin and VHS domains that facilitate trafficking between the trans-Golgi network and the vacuole/lysosome."
Hirst J., Lui W.W.Y., Bright N.A., Totty N., Seaman M.N.J., Robinson M.S.
J. Cell Biol. 149:67-80(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[4]"Yeast GGA proteins interact with GTP-bound Arf and facilitate transport through the Golgi."
Zhdankina O., Strand N.L., Redmond J.M., Boman A.L.
Yeast 18:1-18(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, SUBUNIT.
[5]"Yeast epsin-related proteins required for Golgi-endosome traffic define a gamma-adaptin ear-binding motif."
Duncan M.C., Costaguta G., Payne G.S.
Nat. Cell Biol. 5:77-81(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH ENT3 AND ENT5, MUTAGENESIS OF LYS-560; TRP-563 AND LYS-564.
[6]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[7]"Sites of ubiquitin attachment in Saccharomyces cerevisiae."
Starita L.M., Lo R.S., Eng J.K., von Haller P.D., Fields S.
Proteomics 12:236-240(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U00059 Genomic DNA. Translation: AAB68854.1.
BK006934 Genomic DNA. Translation: DAA06802.1.
PIRS48950.
RefSeqNP_011976.1. NM_001179238.1.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
3MNMX-ray1.73A/B/C465-585[»]
ProteinModelPortalP38817.
SMRP38817. Positions 24-169, 192-323, 471-585.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid36541. 86 interactions.
DIPDIP-2971N.
IntActP38817. 19 interactions.
MINTMINT-634509.
STRING4932.YHR108W.

Proteomic databases

PaxDbP38817.
PeptideAtlasP38817.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYHR108W; YHR108W; YHR108W.
GeneID856508.
KEGGsce:YHR108W.

Organism-specific databases

CYGDYHR108w.
SGDS000001150. GGA2.

Phylogenomic databases

eggNOGNOG321636.
GeneTreeENSGT00700000104396.
HOGENOMHOG000209783.
OMAMCEEDSE.
OrthoDBEOG7GJ6PG.

Enzyme and pathway databases

BioCycYEAST:G3O-31151-MONOMER.

Gene expression databases

GenevestigatorP38817.

Family and domain databases

Gene3D1.25.40.90. 1 hit.
2.60.40.1230. 1 hit.
InterProIPR008152. Clathrin_a/b/g-adaptin_app_Ig.
IPR008153. Clathrin_g-adaptin_app.
IPR013041. Coatomer/clathrin_app_Ig-like.
IPR008942. ENTH_VHS.
IPR004152. GAT.
IPR002014. VHS.
IPR018205. VHS_subgr.
[Graphical view]
PfamPF02883. Alpha_adaptinC2. 1 hit.
PF03127. GAT. 1 hit.
PF00790. VHS. 1 hit.
[Graphical view]
SMARTSM00809. Alpha_adaptinC2. 1 hit.
SM00288. VHS. 1 hit.
[Graphical view]
SUPFAMSSF48464. SSF48464. 1 hit.
SSF49348. SSF49348. 1 hit.
PROSITEPS50180. GAE. 1 hit.
PS50909. GAT. 1 hit.
PS50179. VHS. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio982242.
PROP38817.

Entry information

Entry nameGGA2_YEAST
AccessionPrimary (citable) accession number: P38817
Secondary accession number(s): D3DL58
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: February 1, 1995
Last modified: April 16, 2014
This is version 118 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome VIII

Yeast (Saccharomyces cerevisiae) chromosome VIII: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references