ADP-ribosylation factor-binding protein GGA2 - Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

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P38817 (GGA2_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 108. History...

Clusters with 100%, 90%, 50% identity |

Documents (4) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
ADP-ribosylation factor-binding protein GGA2

Alternative name(s):
Golgi-localized, gamma ear-containing, ARF-binding protein 2

Gene names

Name:	GGA2
Ordered Locus Names:	YHR108W

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces ›

Protein attributes

Sequence length	585 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	May play a role in the regulation of membrane traffic through the trans-Golgi network. Ref.3 Ref.4
Subunit structure	Binds to ARF1 and ARF2. Ref.4 Ref.5
Subcellular location	Golgi apparatus › trans-Golgi network.
Miscellaneous	Present with 2270 molecules/cell in log phase SD medium.
Sequence similarities	Contains 1 GAE domain. Contains 1 GAT domain. Contains 1 VHS domain.

Ontologies

Keywords
Biological process	Protein transport Transport
Cellular component	Golgi apparatus
Domain	Coiled coil
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	Golgi to endosome transport Inferred from mutant phenotype PubMed 15039776. Source: SGD Golgi to vacuole transport Inferred from physical interaction Ref.3. Source: SGD protein targeting to vacuole involved in ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway Inferred from mutant phenotype PubMed 15039776. Source: SGD
Cellular_component	Golgi apparatus part Inferred from electronic annotation. Source: InterPro clathrin adaptor complex Inferred from electronic annotation. Source: InterPro trans-Golgi network Inferred from direct assay Ref.3. Source: SGD
Molecular_function	phosphatidylinositol-4-phosphate binding Inferred from mutant phenotype PubMed 18287542. Source: SGD ubiquitin binding Inferred from direct assay PubMed 15039776. Source: SGD
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 585

585

ADP-ribosylation factor-binding protein GGA2

PRO_0000212687

Regions

Domain

33 – 169

137

VHS

Domain

196 – 321

126

GAT

Domain

466 – 581

116

GAE

Experimental info

Mutagenesis

560

K → Q: Reduced binding to ENT3. Ref.5

Mutagenesis

563

W → A: Reduced binding to ENT3 and ENT5. Ref.5

Mutagenesis

564

K → Q: Reduced binding to ENT3 and ENT5. Ref.5

Secondary structure

585

Helix Strand Turn

Details...

Sequences

Sequence

Length

Mass (Da)

Tools

P38817 [UniParc].

Last modified February 1, 1995. Version 1.
Checksum: 7AC3A317AEE302E2
Show »

FASTA

585

64,347

        10         20         30         40         50         60 
MSHPHSHSIY LSELPVRKPQ ALGNPLLRKI QRACRMSLAE PDLALNLDIA DYINEKQGAA 

        70         80         90        100        110        120 
PRDAAIALAK LINNRESHVA IFALSLLDVL VKNCGYPFHL QISRKEFLNE LVKRFPGHPP 

       130        140        150        160        170        180 
LRYSKIQRLI LTAIEEWYQT ICKHSSYKND MGYIRDMHRL LKYKGYAFPK ISESDLAVLK 

       190        200        210        220        230        240 
PSNQLKTASE IQKEQEIAQA AKLEELIRRG KPEDLREANK LMKIMAGFKE DNAVQAKQAI 

       250        260        270        280        290        300 
SSELNKLKRK ADLLNEMLES PDSQNWDNET TQELHSALKV AQPKFQKIIE EEQEDDALVQ 

       310        320        330        340        350        360 
DLLKFNDTVN QLLEKFNLLK NGDSNAASQI HPSHVSAPLQ QSSGALTNEI NLIDFNDLDE 

       370        380        390        400        410        420 
APSQGNNNTN GTGTPAAAET SVNDLLGDLT DLSISNPSTA NQASFGLGGD IVLGSSQPAP 

       430        440        450        460        470        480 
PVTTTNNSNN TLDLLGLSTP QSPTNSQAVN SSGFDLLMGF NPTTGTTTAP ARTLVNQSPN 

       490        500        510        520        530        540 
LKIEFEISRE SNSVIRIKSF FTNLSSSPIS NLVFLLAVPK SMSLKLQPQS SNFMIGNAKD 

       550        560        570        580 
GISQEGTIEN APANPSKALK VKWKVNYSVN STQAEETAVF TLPNV

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Complete nucleotide sequence of Saccharomyces cerevisiae chromosome VIII." Johnston M., Andrews S., Brinkman R., Cooper J., Ding H., Dover J., Du Z., Favello A., Fulton L., Gattung S., Geisel C., Kirsten J., Kucaba T., Hillier L.W., Jier M., Johnston L., Langston Y., Latreille P. Vaudin M. Science 265:2077-2082(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[2]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[3]	"A family of proteins with gamma-adaptin and VHS domains that facilitate trafficking between the trans-Golgi network and the vacuole/lysosome." Hirst J., Lui W.W.Y., Bright N.A., Totty N., Seaman M.N.J., Robinson M.S. J. Cell Biol. 149:67-80(2000) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
[4]	"Yeast GGA proteins interact with GTP-bound Arf and facilitate transport through the Golgi." Zhdankina O., Strand N.L., Redmond J.M., Boman A.L. Yeast 18:1-18(2001) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, SUBUNIT.
[5]	"Yeast epsin-related proteins required for Golgi-endosome traffic define a gamma-adaptin ear-binding motif." Duncan M.C., Costaguta G., Payne G.S. Nat. Cell Biol. 5:77-81(2003) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH ENT3 AND ENT5, MUTAGENESIS OF LYS-560; TRP-563 AND LYS-564.
[6]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

U00059 Genomic DNA. Translation: AAB68854.1.
BK006934 Genomic DNA. Translation: DAA06802.1.

PIR

S48950.

RefSeq

NP_011976.1. NM_001179238.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
3MNM	X-ray	1.73	A/B/C	465-585	[»]

ProteinModelPortal

P38817.

SMR

P38817. Positions 24-169, 471-585.

ModBase

Search...

Protein-protein interaction databases

DIP

DIP-2971N.

IntAct

P38817. 15 interactions.

MINT

MINT-634509.

STRING

4932.YHR108W.

Proteomic databases

PaxDb

P38817.

PeptideAtlas

P38817.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

EnsemblFungi

YHR108W; YHR108W; YHR108W.

GeneID

856508.

KEGG

sce:YHR108W.

Organism-specific databases

CYGD

YHR108w.

SGD

S000001150. GGA2.

Phylogenomic databases

eggNOG

NOG321636.

GeneTree

ENSGT00700000104396.

HOGENOM

HOG000209783.

OMA

GYIRDMH.

OrthoDB

EOG4VQDXJ.

Gene expression databases

Genevestigator

P38817.

GermOnline

YHR108W. Saccharomyces cerevisiae.

Family and domain databases

Gene3D

1.25.40.90. 1 hit.
2.60.40.1230. 1 hit.

InterPro

IPR008152. Clathrin_a/b/g-adaptin_app_Ig.
IPR008153. Clathrin_g-adaptin_app.
IPR013041. Coatomer/clathrin_app_Ig-like.
IPR008942. ENTH_VHS.
IPR004152. GAT.
IPR002014. VHS.
IPR018205. VHS_subgr.
[Graphical view]

Pfam

PF02883. Alpha_adaptinC2. 1 hit.
PF03127. GAT. 1 hit.
PF00790. VHS. 1 hit.
[Graphical view]

SMART

SM00809. Alpha_adaptinC2. 1 hit.
SM00288. VHS. 1 hit.
[Graphical view]

SUPFAM

SSF49348. Clath_adapt. 1 hit.
SSF48464. ENTH_VHS. 1 hit.

PROSITE

PS50180. GAE. 1 hit.
PS50909. GAT. 1 hit.
PS50179. VHS. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

NextBio

982242.

Entry information

Entry name

GGA2_YEAST

Accession

Primary (citable) accession number: P38817
Secondary accession number(s): D3DL58

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1995
Last sequence update:	February 1, 1995
Last modified:	May 1, 2013
This is version 108 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program

Relevant documents

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

Yeast chromosome VIII

Yeast (Saccharomyces cerevisiae) chromosome VIII: entries and gene names

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Experimental info

Secondary structure

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents