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P38817

- GGA2_YEAST

UniProt

P38817 - GGA2_YEAST

Protein

ADP-ribosylation factor-binding protein GGA2

Gene

GGA2

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 122 (01 Oct 2014)
      Sequence version 1 (01 Feb 1995)
      Previous versions | rss
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    Functioni

    May play a role in the regulation of membrane traffic through the trans-Golgi network.2 Publications

    GO - Molecular functioni

    1. identical protein binding Source: IntAct
    2. phosphatidylinositol-4-phosphate binding Source: SGD
    3. protein binding Source: IntAct
    4. ubiquitin binding Source: SGD

    GO - Biological processi

    1. Golgi to endosome transport Source: SGD
    2. Golgi to vacuole transport Source: SGD
    3. protein targeting to vacuole involved in ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway Source: SGD

    Keywords - Biological processi

    Protein transport, Transport

    Enzyme and pathway databases

    BioCyciYEAST:G3O-31151-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    ADP-ribosylation factor-binding protein GGA2
    Alternative name(s):
    Golgi-localized, gamma ear-containing, ARF-binding protein 2
    Gene namesi
    Name:GGA2
    Ordered Locus Names:YHR108W
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome VIII

    Organism-specific databases

    CYGDiYHR108w.
    SGDiS000001150. GGA2.

    Subcellular locationi

    GO - Cellular componenti

    1. clathrin adaptor complex Source: InterPro
    2. trans-Golgi network Source: SGD

    Keywords - Cellular componenti

    Golgi apparatus

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi560 – 5601K → Q: Reduced binding to ENT3. 1 Publication
    Mutagenesisi563 – 5631W → A: Reduced binding to ENT3 and ENT5. 1 Publication
    Mutagenesisi564 – 5641K → Q: Reduced binding to ENT3 and ENT5. 1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 585585ADP-ribosylation factor-binding protein GGA2PRO_0000212687Add
    BLAST

    Proteomic databases

    MaxQBiP38817.
    PaxDbiP38817.
    PeptideAtlasiP38817.

    Expressioni

    Gene expression databases

    GenevestigatoriP38817.

    Interactioni

    Subunit structurei

    Binds to ARF1 and ARF2.1 Publication

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    itself2EBI-7569,EBI-7569
    MON2P485633EBI-7569,EBI-28333

    Protein-protein interaction databases

    BioGridi36541. 84 interactions.
    DIPiDIP-2971N.
    IntActiP38817. 19 interactions.
    MINTiMINT-634509.
    STRINGi4932.YHR108W.

    Structurei

    Secondary structure

    1
    585
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi472 – 4776
    Beta strandi479 – 48911
    Beta strandi491 – 4933
    Beta strandi495 – 5039
    Beta strandi505 – 5073
    Beta strandi509 – 5179
    Beta strandi522 – 5265
    Beta strandi542 – 5509
    Beta strandi560 – 56910
    Beta strandi572 – 58110

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    3MNMX-ray1.73A/B/C465-585[»]
    ProteinModelPortaliP38817.
    SMRiP38817. Positions 24-169, 471-585.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini33 – 169137VHSPROSITE-ProRule annotationAdd
    BLAST
    Domaini196 – 321126GATPROSITE-ProRule annotationAdd
    BLAST
    Domaini466 – 581116GAEPROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Contains 1 GAE domain.PROSITE-ProRule annotation
    Contains 1 GAT domain.PROSITE-ProRule annotation
    Contains 1 VHS domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Coiled coil

    Phylogenomic databases

    eggNOGiNOG321636.
    GeneTreeiENSGT00700000104396.
    HOGENOMiHOG000209783.
    OMAiMCEEDSE.
    OrthoDBiEOG7GJ6PG.

    Family and domain databases

    Gene3Di1.25.40.90. 1 hit.
    2.60.40.1230. 1 hit.
    InterProiIPR008152. Clathrin_a/b/g-adaptin_app_Ig.
    IPR008153. Clathrin_g-adaptin_app.
    IPR013041. Coatomer/clathrin_app_Ig-like.
    IPR008942. ENTH_VHS.
    IPR004152. GAT.
    IPR002014. VHS.
    IPR018205. VHS_subgr.
    [Graphical view]
    PfamiPF02883. Alpha_adaptinC2. 1 hit.
    PF03127. GAT. 1 hit.
    PF00790. VHS. 1 hit.
    [Graphical view]
    SMARTiSM00809. Alpha_adaptinC2. 1 hit.
    SM00288. VHS. 1 hit.
    [Graphical view]
    SUPFAMiSSF48464. SSF48464. 1 hit.
    SSF49348. SSF49348. 1 hit.
    PROSITEiPS50180. GAE. 1 hit.
    PS50909. GAT. 1 hit.
    PS50179. VHS. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P38817-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSHPHSHSIY LSELPVRKPQ ALGNPLLRKI QRACRMSLAE PDLALNLDIA    50
    DYINEKQGAA PRDAAIALAK LINNRESHVA IFALSLLDVL VKNCGYPFHL 100
    QISRKEFLNE LVKRFPGHPP LRYSKIQRLI LTAIEEWYQT ICKHSSYKND 150
    MGYIRDMHRL LKYKGYAFPK ISESDLAVLK PSNQLKTASE IQKEQEIAQA 200
    AKLEELIRRG KPEDLREANK LMKIMAGFKE DNAVQAKQAI SSELNKLKRK 250
    ADLLNEMLES PDSQNWDNET TQELHSALKV AQPKFQKIIE EEQEDDALVQ 300
    DLLKFNDTVN QLLEKFNLLK NGDSNAASQI HPSHVSAPLQ QSSGALTNEI 350
    NLIDFNDLDE APSQGNNNTN GTGTPAAAET SVNDLLGDLT DLSISNPSTA 400
    NQASFGLGGD IVLGSSQPAP PVTTTNNSNN TLDLLGLSTP QSPTNSQAVN 450
    SSGFDLLMGF NPTTGTTTAP ARTLVNQSPN LKIEFEISRE SNSVIRIKSF 500
    FTNLSSSPIS NLVFLLAVPK SMSLKLQPQS SNFMIGNAKD GISQEGTIEN 550
    APANPSKALK VKWKVNYSVN STQAEETAVF TLPNV 585
    Length:585
    Mass (Da):64,347
    Last modified:February 1, 1995 - v1
    Checksum:i7AC3A317AEE302E2
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U00059 Genomic DNA. Translation: AAB68854.1.
    BK006934 Genomic DNA. Translation: DAA06802.1.
    PIRiS48950.
    RefSeqiNP_011976.1. NM_001179238.1.

    Genome annotation databases

    EnsemblFungiiYHR108W; YHR108W; YHR108W.
    GeneIDi856508.
    KEGGisce:YHR108W.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U00059 Genomic DNA. Translation: AAB68854.1 .
    BK006934 Genomic DNA. Translation: DAA06802.1 .
    PIRi S48950.
    RefSeqi NP_011976.1. NM_001179238.1.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    3MNM X-ray 1.73 A/B/C 465-585 [» ]
    ProteinModelPortali P38817.
    SMRi P38817. Positions 24-169, 471-585.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 36541. 84 interactions.
    DIPi DIP-2971N.
    IntActi P38817. 19 interactions.
    MINTi MINT-634509.
    STRINGi 4932.YHR108W.

    Proteomic databases

    MaxQBi P38817.
    PaxDbi P38817.
    PeptideAtlasi P38817.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YHR108W ; YHR108W ; YHR108W .
    GeneIDi 856508.
    KEGGi sce:YHR108W.

    Organism-specific databases

    CYGDi YHR108w.
    SGDi S000001150. GGA2.

    Phylogenomic databases

    eggNOGi NOG321636.
    GeneTreei ENSGT00700000104396.
    HOGENOMi HOG000209783.
    OMAi MCEEDSE.
    OrthoDBi EOG7GJ6PG.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-31151-MONOMER.

    Miscellaneous databases

    NextBioi 982242.
    PROi P38817.

    Gene expression databases

    Genevestigatori P38817.

    Family and domain databases

    Gene3Di 1.25.40.90. 1 hit.
    2.60.40.1230. 1 hit.
    InterProi IPR008152. Clathrin_a/b/g-adaptin_app_Ig.
    IPR008153. Clathrin_g-adaptin_app.
    IPR013041. Coatomer/clathrin_app_Ig-like.
    IPR008942. ENTH_VHS.
    IPR004152. GAT.
    IPR002014. VHS.
    IPR018205. VHS_subgr.
    [Graphical view ]
    Pfami PF02883. Alpha_adaptinC2. 1 hit.
    PF03127. GAT. 1 hit.
    PF00790. VHS. 1 hit.
    [Graphical view ]
    SMARTi SM00809. Alpha_adaptinC2. 1 hit.
    SM00288. VHS. 1 hit.
    [Graphical view ]
    SUPFAMi SSF48464. SSF48464. 1 hit.
    SSF49348. SSF49348. 1 hit.
    PROSITEi PS50180. GAE. 1 hit.
    PS50909. GAT. 1 hit.
    PS50179. VHS. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    2. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    3. "A family of proteins with gamma-adaptin and VHS domains that facilitate trafficking between the trans-Golgi network and the vacuole/lysosome."
      Hirst J., Lui W.W.Y., Bright N.A., Totty N., Seaman M.N.J., Robinson M.S.
      J. Cell Biol. 149:67-80(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    4. "Yeast GGA proteins interact with GTP-bound Arf and facilitate transport through the Golgi."
      Zhdankina O., Strand N.L., Redmond J.M., Boman A.L.
      Yeast 18:1-18(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, SUBUNIT.
    5. "Yeast epsin-related proteins required for Golgi-endosome traffic define a gamma-adaptin ear-binding motif."
      Duncan M.C., Costaguta G., Payne G.S.
      Nat. Cell Biol. 5:77-81(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH ENT3 AND ENT5, MUTAGENESIS OF LYS-560; TRP-563 AND LYS-564.
    6. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
    7. "Sites of ubiquitin attachment in Saccharomyces cerevisiae."
      Starita L.M., Lo R.S., Eng J.K., von Haller P.D., Fields S.
      Proteomics 12:236-240(2012) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiGGA2_YEAST
    AccessioniPrimary (citable) accession number: P38817
    Secondary accession number(s): D3DL58
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 1, 1995
    Last sequence update: February 1, 1995
    Last modified: October 1, 2014
    This is version 122 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 2270 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families
    3. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    4. Yeast chromosome VIII
      Yeast (Saccharomyces cerevisiae) chromosome VIII: entries and gene names

    External Data

    Dasty 3