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P38817

- GGA2_YEAST

UniProt

P38817 - GGA2_YEAST

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Protein
ADP-ribosylation factor-binding protein GGA2
Gene
GGA2, YHR108W
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

May play a role in the regulation of membrane traffic through the trans-Golgi network.2 Publications

GO - Molecular functioni

  1. identical protein binding Source: IntAct
  2. phosphatidylinositol-4-phosphate binding Source: SGD
  3. protein binding Source: IntAct
  4. ubiquitin binding Source: SGD

GO - Biological processi

  1. Golgi to endosome transport Source: SGD
  2. Golgi to vacuole transport Source: SGD
  3. protein targeting to vacuole involved in ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway Source: SGD
Complete GO annotation...

Keywords - Biological processi

Protein transport, Transport

Enzyme and pathway databases

BioCyciYEAST:G3O-31151-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
ADP-ribosylation factor-binding protein GGA2
Alternative name(s):
Golgi-localized, gamma ear-containing, ARF-binding protein 2
Gene namesi
Name:GGA2
Ordered Locus Names:YHR108W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome VIII

Organism-specific databases

CYGDiYHR108w.
SGDiS000001150. GGA2.

Subcellular locationi

GO - Cellular componenti

  1. clathrin adaptor complex Source: InterPro
  2. trans-Golgi network Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Golgi apparatus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi560 – 5601K → Q: Reduced binding to ENT3. 1 Publication
Mutagenesisi563 – 5631W → A: Reduced binding to ENT3 and ENT5. 1 Publication
Mutagenesisi564 – 5641K → Q: Reduced binding to ENT3 and ENT5. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 585585ADP-ribosylation factor-binding protein GGA2
PRO_0000212687Add
BLAST

Proteomic databases

MaxQBiP38817.
PaxDbiP38817.
PeptideAtlasiP38817.

Expressioni

Gene expression databases

GenevestigatoriP38817.

Interactioni

Subunit structurei

Binds to ARF1 and ARF2.2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
itself2EBI-7569,EBI-7569
MON2P485633EBI-7569,EBI-28333

Protein-protein interaction databases

BioGridi36541. 84 interactions.
DIPiDIP-2971N.
IntActiP38817. 19 interactions.
MINTiMINT-634509.
STRINGi4932.YHR108W.

Structurei

Secondary structure

Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi472 – 4776
Beta strandi479 – 48911
Beta strandi491 – 4933
Beta strandi495 – 5039
Beta strandi505 – 5073
Beta strandi509 – 5179
Beta strandi522 – 5265
Beta strandi542 – 5509
Beta strandi560 – 56910
Beta strandi572 – 58110

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3MNMX-ray1.73A/B/C465-585[»]
ProteinModelPortaliP38817.
SMRiP38817. Positions 24-169, 471-585.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini33 – 169137VHS
Add
BLAST
Domaini196 – 321126GAT
Add
BLAST
Domaini466 – 581116GAE
Add
BLAST

Sequence similaritiesi

Contains 1 GAE domain.
Contains 1 GAT domain.
Contains 1 VHS domain.

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiNOG321636.
GeneTreeiENSGT00700000104396.
HOGENOMiHOG000209783.
OMAiMCEEDSE.
OrthoDBiEOG7GJ6PG.

Family and domain databases

Gene3Di1.25.40.90. 1 hit.
2.60.40.1230. 1 hit.
InterProiIPR008152. Clathrin_a/b/g-adaptin_app_Ig.
IPR008153. Clathrin_g-adaptin_app.
IPR013041. Coatomer/clathrin_app_Ig-like.
IPR008942. ENTH_VHS.
IPR004152. GAT.
IPR002014. VHS.
IPR018205. VHS_subgr.
[Graphical view]
PfamiPF02883. Alpha_adaptinC2. 1 hit.
PF03127. GAT. 1 hit.
PF00790. VHS. 1 hit.
[Graphical view]
SMARTiSM00809. Alpha_adaptinC2. 1 hit.
SM00288. VHS. 1 hit.
[Graphical view]
SUPFAMiSSF48464. SSF48464. 1 hit.
SSF49348. SSF49348. 1 hit.
PROSITEiPS50180. GAE. 1 hit.
PS50909. GAT. 1 hit.
PS50179. VHS. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P38817-1 [UniParc]FASTAAdd to Basket

« Hide

MSHPHSHSIY LSELPVRKPQ ALGNPLLRKI QRACRMSLAE PDLALNLDIA    50
DYINEKQGAA PRDAAIALAK LINNRESHVA IFALSLLDVL VKNCGYPFHL 100
QISRKEFLNE LVKRFPGHPP LRYSKIQRLI LTAIEEWYQT ICKHSSYKND 150
MGYIRDMHRL LKYKGYAFPK ISESDLAVLK PSNQLKTASE IQKEQEIAQA 200
AKLEELIRRG KPEDLREANK LMKIMAGFKE DNAVQAKQAI SSELNKLKRK 250
ADLLNEMLES PDSQNWDNET TQELHSALKV AQPKFQKIIE EEQEDDALVQ 300
DLLKFNDTVN QLLEKFNLLK NGDSNAASQI HPSHVSAPLQ QSSGALTNEI 350
NLIDFNDLDE APSQGNNNTN GTGTPAAAET SVNDLLGDLT DLSISNPSTA 400
NQASFGLGGD IVLGSSQPAP PVTTTNNSNN TLDLLGLSTP QSPTNSQAVN 450
SSGFDLLMGF NPTTGTTTAP ARTLVNQSPN LKIEFEISRE SNSVIRIKSF 500
FTNLSSSPIS NLVFLLAVPK SMSLKLQPQS SNFMIGNAKD GISQEGTIEN 550
APANPSKALK VKWKVNYSVN STQAEETAVF TLPNV 585
Length:585
Mass (Da):64,347
Last modified:February 1, 1995 - v1
Checksum:i7AC3A317AEE302E2
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U00059 Genomic DNA. Translation: AAB68854.1.
BK006934 Genomic DNA. Translation: DAA06802.1.
PIRiS48950.
RefSeqiNP_011976.1. NM_001179238.1.

Genome annotation databases

EnsemblFungiiYHR108W; YHR108W; YHR108W.
GeneIDi856508.
KEGGisce:YHR108W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U00059 Genomic DNA. Translation: AAB68854.1 .
BK006934 Genomic DNA. Translation: DAA06802.1 .
PIRi S48950.
RefSeqi NP_011976.1. NM_001179238.1.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
3MNM X-ray 1.73 A/B/C 465-585 [» ]
ProteinModelPortali P38817.
SMRi P38817. Positions 24-169, 471-585.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 36541. 84 interactions.
DIPi DIP-2971N.
IntActi P38817. 19 interactions.
MINTi MINT-634509.
STRINGi 4932.YHR108W.

Proteomic databases

MaxQBi P38817.
PaxDbi P38817.
PeptideAtlasi P38817.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YHR108W ; YHR108W ; YHR108W .
GeneIDi 856508.
KEGGi sce:YHR108W.

Organism-specific databases

CYGDi YHR108w.
SGDi S000001150. GGA2.

Phylogenomic databases

eggNOGi NOG321636.
GeneTreei ENSGT00700000104396.
HOGENOMi HOG000209783.
OMAi MCEEDSE.
OrthoDBi EOG7GJ6PG.

Enzyme and pathway databases

BioCyci YEAST:G3O-31151-MONOMER.

Miscellaneous databases

NextBioi 982242.
PROi P38817.

Gene expression databases

Genevestigatori P38817.

Family and domain databases

Gene3Di 1.25.40.90. 1 hit.
2.60.40.1230. 1 hit.
InterProi IPR008152. Clathrin_a/b/g-adaptin_app_Ig.
IPR008153. Clathrin_g-adaptin_app.
IPR013041. Coatomer/clathrin_app_Ig-like.
IPR008942. ENTH_VHS.
IPR004152. GAT.
IPR002014. VHS.
IPR018205. VHS_subgr.
[Graphical view ]
Pfami PF02883. Alpha_adaptinC2. 1 hit.
PF03127. GAT. 1 hit.
PF00790. VHS. 1 hit.
[Graphical view ]
SMARTi SM00809. Alpha_adaptinC2. 1 hit.
SM00288. VHS. 1 hit.
[Graphical view ]
SUPFAMi SSF48464. SSF48464. 1 hit.
SSF49348. SSF49348. 1 hit.
PROSITEi PS50180. GAE. 1 hit.
PS50909. GAT. 1 hit.
PS50179. VHS. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  2. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  3. "A family of proteins with gamma-adaptin and VHS domains that facilitate trafficking between the trans-Golgi network and the vacuole/lysosome."
    Hirst J., Lui W.W.Y., Bright N.A., Totty N., Seaman M.N.J., Robinson M.S.
    J. Cell Biol. 149:67-80(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  4. "Yeast GGA proteins interact with GTP-bound Arf and facilitate transport through the Golgi."
    Zhdankina O., Strand N.L., Redmond J.M., Boman A.L.
    Yeast 18:1-18(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBUNIT.
  5. "Yeast epsin-related proteins required for Golgi-endosome traffic define a gamma-adaptin ear-binding motif."
    Duncan M.C., Costaguta G., Payne G.S.
    Nat. Cell Biol. 5:77-81(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH ENT3 AND ENT5, MUTAGENESIS OF LYS-560; TRP-563 AND LYS-564.
  6. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  7. "Sites of ubiquitin attachment in Saccharomyces cerevisiae."
    Starita L.M., Lo R.S., Eng J.K., von Haller P.D., Fields S.
    Proteomics 12:236-240(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiGGA2_YEAST
AccessioniPrimary (citable) accession number: P38817
Secondary accession number(s): D3DL58
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: February 1, 1995
Last modified: July 9, 2014
This is version 121 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 2270 molecules/cell in log phase SD medium.

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome VIII
    Yeast (Saccharomyces cerevisiae) chromosome VIII: entries and gene names

External Data

Dasty 3

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