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P38817

- GGA2_YEAST

UniProt

P38817 - GGA2_YEAST

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Protein

ADP-ribosylation factor-binding protein GGA2

Gene

GGA2

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

May play a role in the regulation of membrane traffic through the trans-Golgi network.2 Publications

GO - Molecular functioni

  1. identical protein binding Source: IntAct
  2. phosphatidylinositol-4-phosphate binding Source: SGD
  3. ubiquitin binding Source: SGD

GO - Biological processi

  1. Golgi to endosome transport Source: SGD
  2. Golgi to vacuole transport Source: SGD
  3. protein targeting to vacuole involved in ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway Source: SGD
Complete GO annotation...

Keywords - Biological processi

Protein transport, Transport

Enzyme and pathway databases

BioCyciYEAST:G3O-31151-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
ADP-ribosylation factor-binding protein GGA2
Alternative name(s):
Golgi-localized, gamma ear-containing, ARF-binding protein 2
Gene namesi
Name:GGA2
Ordered Locus Names:YHR108W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome VIII

Organism-specific databases

CYGDiYHR108w.
SGDiS000001150. GGA2.

Subcellular locationi

GO - Cellular componenti

  1. clathrin adaptor complex Source: InterPro
  2. trans-Golgi network Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Golgi apparatus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi560 – 5601K → Q: Reduced binding to ENT3. 1 Publication
Mutagenesisi563 – 5631W → A: Reduced binding to ENT3 and ENT5. 1 Publication
Mutagenesisi564 – 5641K → Q: Reduced binding to ENT3 and ENT5. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 585585ADP-ribosylation factor-binding protein GGA2PRO_0000212687Add
BLAST

Proteomic databases

MaxQBiP38817.
PaxDbiP38817.
PeptideAtlasiP38817.

Expressioni

Gene expression databases

GenevestigatoriP38817.

Interactioni

Subunit structurei

Binds to ARF1 and ARF2.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
itself2EBI-7569,EBI-7569
MON2P485633EBI-7569,EBI-28333

Protein-protein interaction databases

BioGridi36541. 85 interactions.
DIPiDIP-2971N.
IntActiP38817. 19 interactions.
MINTiMINT-634509.
STRINGi4932.YHR108W.

Structurei

Secondary structure

1
585
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi472 – 4776Combined sources
Beta strandi479 – 48911Combined sources
Beta strandi491 – 4933Combined sources
Beta strandi495 – 5039Combined sources
Beta strandi505 – 5073Combined sources
Beta strandi509 – 5179Combined sources
Beta strandi522 – 5265Combined sources
Beta strandi542 – 5509Combined sources
Beta strandi560 – 56910Combined sources
Beta strandi572 – 58110Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3MNMX-ray1.73A/B/C465-585[»]
ProteinModelPortaliP38817.
SMRiP38817. Positions 24-169, 471-585.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini33 – 169137VHSPROSITE-ProRule annotationAdd
BLAST
Domaini196 – 321126GATPROSITE-ProRule annotationAdd
BLAST
Domaini466 – 581116GAEPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 GAE domain.PROSITE-ProRule annotation
Contains 1 GAT domain.PROSITE-ProRule annotation
Contains 1 VHS domain.PROSITE-ProRule annotation

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiNOG321636.
GeneTreeiENSGT00700000104396.
HOGENOMiHOG000209783.
InParanoidiP38817.
OMAiMCEEDSE.
OrthoDBiEOG7GJ6PG.

Family and domain databases

Gene3Di1.25.40.90. 1 hit.
2.60.40.1230. 1 hit.
InterProiIPR008152. Clathrin_a/b/g-adaptin_app_Ig.
IPR008153. Clathrin_g-adaptin_app.
IPR013041. Coatomer/clathrin_app_Ig-like.
IPR008942. ENTH_VHS.
IPR004152. GAT.
IPR002014. VHS.
IPR018205. VHS_subgr.
[Graphical view]
PfamiPF02883. Alpha_adaptinC2. 1 hit.
PF03127. GAT. 1 hit.
PF00790. VHS. 1 hit.
[Graphical view]
SMARTiSM00809. Alpha_adaptinC2. 1 hit.
SM00288. VHS. 1 hit.
[Graphical view]
SUPFAMiSSF48464. SSF48464. 1 hit.
SSF49348. SSF49348. 1 hit.
PROSITEiPS50180. GAE. 1 hit.
PS50909. GAT. 1 hit.
PS50179. VHS. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P38817-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MSHPHSHSIY LSELPVRKPQ ALGNPLLRKI QRACRMSLAE PDLALNLDIA
60 70 80 90 100
DYINEKQGAA PRDAAIALAK LINNRESHVA IFALSLLDVL VKNCGYPFHL
110 120 130 140 150
QISRKEFLNE LVKRFPGHPP LRYSKIQRLI LTAIEEWYQT ICKHSSYKND
160 170 180 190 200
MGYIRDMHRL LKYKGYAFPK ISESDLAVLK PSNQLKTASE IQKEQEIAQA
210 220 230 240 250
AKLEELIRRG KPEDLREANK LMKIMAGFKE DNAVQAKQAI SSELNKLKRK
260 270 280 290 300
ADLLNEMLES PDSQNWDNET TQELHSALKV AQPKFQKIIE EEQEDDALVQ
310 320 330 340 350
DLLKFNDTVN QLLEKFNLLK NGDSNAASQI HPSHVSAPLQ QSSGALTNEI
360 370 380 390 400
NLIDFNDLDE APSQGNNNTN GTGTPAAAET SVNDLLGDLT DLSISNPSTA
410 420 430 440 450
NQASFGLGGD IVLGSSQPAP PVTTTNNSNN TLDLLGLSTP QSPTNSQAVN
460 470 480 490 500
SSGFDLLMGF NPTTGTTTAP ARTLVNQSPN LKIEFEISRE SNSVIRIKSF
510 520 530 540 550
FTNLSSSPIS NLVFLLAVPK SMSLKLQPQS SNFMIGNAKD GISQEGTIEN
560 570 580
APANPSKALK VKWKVNYSVN STQAEETAVF TLPNV
Length:585
Mass (Da):64,347
Last modified:February 1, 1995 - v1
Checksum:i7AC3A317AEE302E2
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00059 Genomic DNA. Translation: AAB68854.1.
BK006934 Genomic DNA. Translation: DAA06802.1.
PIRiS48950.
RefSeqiNP_011976.1. NM_001179238.1.

Genome annotation databases

EnsemblFungiiYHR108W; YHR108W; YHR108W.
GeneIDi856508.
KEGGisce:YHR108W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00059 Genomic DNA. Translation: AAB68854.1 .
BK006934 Genomic DNA. Translation: DAA06802.1 .
PIRi S48950.
RefSeqi NP_011976.1. NM_001179238.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
3MNM X-ray 1.73 A/B/C 465-585 [» ]
ProteinModelPortali P38817.
SMRi P38817. Positions 24-169, 471-585.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 36541. 85 interactions.
DIPi DIP-2971N.
IntActi P38817. 19 interactions.
MINTi MINT-634509.
STRINGi 4932.YHR108W.

Proteomic databases

MaxQBi P38817.
PaxDbi P38817.
PeptideAtlasi P38817.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YHR108W ; YHR108W ; YHR108W .
GeneIDi 856508.
KEGGi sce:YHR108W.

Organism-specific databases

CYGDi YHR108w.
SGDi S000001150. GGA2.

Phylogenomic databases

eggNOGi NOG321636.
GeneTreei ENSGT00700000104396.
HOGENOMi HOG000209783.
InParanoidi P38817.
OMAi MCEEDSE.
OrthoDBi EOG7GJ6PG.

Enzyme and pathway databases

BioCyci YEAST:G3O-31151-MONOMER.

Miscellaneous databases

NextBioi 982242.
PROi P38817.

Gene expression databases

Genevestigatori P38817.

Family and domain databases

Gene3Di 1.25.40.90. 1 hit.
2.60.40.1230. 1 hit.
InterProi IPR008152. Clathrin_a/b/g-adaptin_app_Ig.
IPR008153. Clathrin_g-adaptin_app.
IPR013041. Coatomer/clathrin_app_Ig-like.
IPR008942. ENTH_VHS.
IPR004152. GAT.
IPR002014. VHS.
IPR018205. VHS_subgr.
[Graphical view ]
Pfami PF02883. Alpha_adaptinC2. 1 hit.
PF03127. GAT. 1 hit.
PF00790. VHS. 1 hit.
[Graphical view ]
SMARTi SM00809. Alpha_adaptinC2. 1 hit.
SM00288. VHS. 1 hit.
[Graphical view ]
SUPFAMi SSF48464. SSF48464. 1 hit.
SSF49348. SSF49348. 1 hit.
PROSITEi PS50180. GAE. 1 hit.
PS50909. GAT. 1 hit.
PS50179. VHS. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  2. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  3. "A family of proteins with gamma-adaptin and VHS domains that facilitate trafficking between the trans-Golgi network and the vacuole/lysosome."
    Hirst J., Lui W.W.Y., Bright N.A., Totty N., Seaman M.N.J., Robinson M.S.
    J. Cell Biol. 149:67-80(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  4. "Yeast GGA proteins interact with GTP-bound Arf and facilitate transport through the Golgi."
    Zhdankina O., Strand N.L., Redmond J.M., Boman A.L.
    Yeast 18:1-18(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBUNIT.
  5. "Yeast epsin-related proteins required for Golgi-endosome traffic define a gamma-adaptin ear-binding motif."
    Duncan M.C., Costaguta G., Payne G.S.
    Nat. Cell Biol. 5:77-81(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH ENT3 AND ENT5, MUTAGENESIS OF LYS-560; TRP-563 AND LYS-564.
  6. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  7. "Sites of ubiquitin attachment in Saccharomyces cerevisiae."
    Starita L.M., Lo R.S., Eng J.K., von Haller P.D., Fields S.
    Proteomics 12:236-240(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiGGA2_YEAST
AccessioniPrimary (citable) accession number: P38817
Secondary accession number(s): D3DL58
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: February 1, 1995
Last modified: November 26, 2014
This is version 124 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 2270 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome VIII
    Yeast (Saccharomyces cerevisiae) chromosome VIII: entries and gene names

External Data

Dasty 3