Reviewed, UniProtKB/Swiss-Prot P38816 (TRXB2_YEAST)

Names and origin

Protein names

Recommended name:
    Thioredoxin reductase 2, mitochondrial
    EC=1.8.1.9

Gene names

Name:	TRR2
Ordered Locus Names:	YHR106W

Organism

Saccharomyces cerevisiae (Baker's yeast) [Complete proteome]

Taxonomic identifier

4932 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	342 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Acts on mitochondrial thioredoxin 3. Implicated in the defense against oxidative stress.
Catalytic activity	Thioredoxin + NADP+ = thioredoxin disulfide + NADPH.
Cofactor	Binds 1 FAD per subunit By similarity.
Subunit structure	Homodimer.
Subcellular location	Mitochondrion.
Miscellaneous	Present with 414 molecules/cell in log phase SD medium. Ref.4
Sequence similarities	Belongs to the class-II pyridine nucleotide-disulfide oxidoreductase family.

Ontologies

Keywords
Cellular component	Mitochondrion
Domain	Redox-active center Transit peptide
Ligand	FAD Flavoprotein NADP
Molecular function	Oxidoreductase
PTM	Disulfide bond
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cell redox homeostasis Ref.3 Inferred by curator. Source: SGD oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW removal of superoxide radicals Inferred from electronic annotation. Source: InterPro response to oxidative stress Ref.3 Inferred from mutant phenotype. Source: SGD
Cellular component	mitochondrion Ref.3 Inferred from direct assay. Source: SGD
Molecular function	FAD binding Inferred from electronic annotation. Source: InterPro electron carrier activity Inferred from electronic annotation. Source: InterPro thioredoxin-disulfide reductase activity Ref.3 Inferred from direct assay. Source: SGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – 23	23	Mitochondrion Potential
	Chain	24 – 342	319	Thioredoxin reductase 2, mitochondrial		PRO_0000030303
Regions
	Nucleotide binding	56 – 68	13	FAD By similarity
	Nucleotide binding	311 – 320	10	FAD By similarity
Amino acid modifications
	Disulfide bond	165 ↔ 168		Redox-active By similarity

Sequences

	Sequence		Length	Mass (Da)
	P38816-1 [UniParc]. Last modified February 1, 1995. Version 1. Checksum: 739F302AA0837A5A	FASTA	342	37,087
	10 20 30 40 50 60 MIKHIVSPFR TNFVGISKSV LSRMIHHKVT IIGSGPAAHT AAIYLARAEM KPTLYEGMMA 70 80 90 100 110 120 NGIAAGGQLT TTTDIENFPG FPESLSGSEL MERMRKQSAK FGTNIITETV SKVDLSSKPF 130 140 150 160 170 180 RLWTEFNEDA EPVTTDAIIL ATGASAKRMH LPGEETYWQQ GISACAVCDG AVPIFRNKPL 190 200 210 220 230 240 AVIGGGDSAC EEAEFLTKYA SKVYILVRKD HFRASVIMQR RIEKNPNIIV LFNTVALEAK 250 260 270 280 290 300 GDGKLLNMLR IKNTKSNVEN DLEVNGLFYA IGHSPATDIV KGQVDEEETG YIKTVPGSSL 310 320 330 340 TSVPGFFAAG DVQDSRYRQA VTSAGSGCIA ALDAERYLSA QE

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Complete nucleotide sequence of Saccharomyces cerevisiae chromosome VIII." Johnston M., Andrews S., Brinkman R., Cooper J., Ding H., Dover J., Du Z., Favello A., Fulton L., Gattung S., Geisel C., Kirsten J., Kucaba T., Hillier L.W., Jier M., Johnston L., Langston Y., Latreille P. , Louis E.J., Macri C., Mardis E., Menezes S., Mouser L., Nhan M., Rifkin L., Riles L., St Peter H., Trevaskis E., Vaughan K., Vignati D., Wilcox L., Wohldman P., Waterston R., Wilson R., Vaudin M. Science 265:2077-2082(1994) [PubMed: 8091229] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[2]	"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae." Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J. Genome Res. 17:536-543(2007) [PubMed: 17322287] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[3]	"Identification and functional characterization of a novel mitochondrial thioredoxin system in Saccharomyces cerevisiae." Pedrajas J.R., Kosmidou E., Miranda-Vizuete A., Gustafsson J.-A., Wright A.P.H., Spyrou G. J. Biol. Chem. 274:6366-6373(1999) [PubMed: 10037727] [Abstract] Cited for: CHARACTERIZATION.
[4]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	U00059 Genomic DNA. Translation: AAB68856.1. AY557882 Genomic DNA. Translation: AAS56208.1.
PIR	S48948.
RefSeq	NP_011974.1.
3D structure databases
HSSP	HSSP built from PDB template 1VDC based on UniProtKB Q39243.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP:1942N.
IntAct	P38816. 6 interactions.
Proteomic databases
PeptideAtlas	P38816.
Genome annotation databases
Ensembl	YHR106W. Saccharomyces cerevisiae. [Contig view]
GeneID	856506.
GenomeReviews	Gene locus YHR106W in contig U00093_GR.
KEGG	sce:YHR106W.
NMPDR	fig|4932.3.peg.3135.
Organism-specific databases
CYGD	YHR106w.
SGD	S000001148. TRR2.
Yeast-GFP	Search...
Phylogenomic databases
HOGENOM	P38816.
OMA	P38816. GEIKKVW.
Enzyme and pathway databases
BRENDA	1.8.1.9. 250.
Gene expression databases
GermOnline	YHR106W. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR013027. FAD_pyr_nucl-diS_OxRdtase. IPR008255. Pyr_nucl-diS_OxRdtase_2_AS. IPR001327. Pyr_OxRdtase_NAD_bd. IPR000103. Pyridine_nuc-diS_OxRdtase_2. IPR005982. Thioredox_Rdtase. [Graphical view]
Pfam	PF00070. Pyr_redox. 1 hit. PF07992. Pyr_redox_2. 1 hit. [Graphical view]
PRINTS	PR00368. FADPNR. PR00469. PNDRDTASEII.
ProDom	PD000139. FAD_pyr_redox. 1 hit. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR01292. TRX_reduct. 1 hit.
PROSITE	PS00573. PYRIDINE_REDOX_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	982236.

Entry information

Entry name

TRXB2_YEAST

Accession

Primary (citable) accession number: P38816

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1995
Last sequence update:	February 1, 1995
Last modified:	June 16, 2009
This is version 86 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)