Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

PX domain-containing protein YPT35

Gene

YPT35

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

GO - Molecular functioni

  • phosphatidylinositol-3-phosphate binding Source: SGD
Complete GO annotation...

Enzyme and pathway databases

BioCyciYEAST:G3O-31149-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
PX domain-containing protein YPT35
Gene namesi
Name:YPT35
Ordered Locus Names:YHR105W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome VIII

Organism-specific databases

EuPathDBiFungiDB:YHR105W.
SGDiS000001147. YPT35.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Endosome, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi123 – 1231Y → A: Abolishes partially PtdIns(3)P-binding. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 214214PX domain-containing protein YPT35PRO_0000202910Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei65 – 651PhosphoserineCombined sources
Modified residuei66 – 661PhosphoserineCombined sources

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP38815.

PTM databases

iPTMnetiP38815.

Interactioni

Subunit structurei

Interacts with RBD2, YIF1, YIP1 and YIP4.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
YIF1P538454EBI-24665,EBI-28230
YIP1P530393EBI-24665,EBI-25295
YIP4P530932EBI-24665,EBI-24124

Protein-protein interaction databases

BioGridi36538. 38 interactions.
DIPiDIP-1881N.
IntActiP38815. 17 interactions.
MINTiMINT-388901.

Structurei

3D structure databases

ProteinModelPortaliP38815.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini73 – 213141PXPROSITE-ProRule annotationAdd
BLAST

Domaini

The PX domain binds phosphatidylinositol 3-phosphate (PtdIns3P) which is necessary for peripheral membrane localization.1 Publication

Sequence similaritiesi

Belongs to the YPT35 family.Curated
Contains 1 PX (phox homology) domain.PROSITE-ProRule annotation

Phylogenomic databases

InParanoidiP38815.
OMAiFAVWRIT.
OrthoDBiEOG7XPZHR.

Family and domain databases

Gene3Di3.30.1520.10. 1 hit.
InterProiIPR001683. Phox.
[Graphical view]
PfamiPF00787. PX. 1 hit.
[Graphical view]
SMARTiSM00312. PX. 1 hit.
[Graphical view]
SUPFAMiSSF64268. SSF64268. 1 hit.
PROSITEiPS50195. PX. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P38815-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSDKISFLPP EPIQLLDEDS TEPELDIDSQ QENEGPISAS NSNDSTSHSN
60 70 80 90 100
DCGATITRTR PRRSSSINAN FSFQKAHVSD CTIVNGDHGT KFAVWRITVF
110 120 130 140 150
LEPNLKAFAA KRESYKIQTY KRYSDFVRLR ENLLTRIKTA KPEKLNCLQI
160 170 180 190 200
PHLPPSVQWY SSWKYQEVNL NKDWLAKRQR GLEYFLNHII LNSSLVEMTK
210
DILIQFLEPS KRVA
Length:214
Mass (Da):24,646
Last modified:February 1, 1995 - v1
Checksum:i2ADD21AF107094DC
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00059 Genomic DNA. Translation: AAB68857.1.
AY557854 Genomic DNA. Translation: AAS56180.1.
BK006934 Genomic DNA. Translation: DAA06799.1.
PIRiS48947.
RefSeqiNP_011973.1. NM_001179235.1.

Genome annotation databases

EnsemblFungiiYHR105W; YHR105W; YHR105W.
GeneIDi856505.
KEGGisce:YHR105W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00059 Genomic DNA. Translation: AAB68857.1.
AY557854 Genomic DNA. Translation: AAS56180.1.
BK006934 Genomic DNA. Translation: DAA06799.1.
PIRiS48947.
RefSeqiNP_011973.1. NM_001179235.1.

3D structure databases

ProteinModelPortaliP38815.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi36538. 38 interactions.
DIPiDIP-1881N.
IntActiP38815. 17 interactions.
MINTiMINT-388901.

PTM databases

iPTMnetiP38815.

Proteomic databases

MaxQBiP38815.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYHR105W; YHR105W; YHR105W.
GeneIDi856505.
KEGGisce:YHR105W.

Organism-specific databases

EuPathDBiFungiDB:YHR105W.
SGDiS000001147. YPT35.

Phylogenomic databases

InParanoidiP38815.
OMAiFAVWRIT.
OrthoDBiEOG7XPZHR.

Enzyme and pathway databases

BioCyciYEAST:G3O-31149-MONOMER.

Miscellaneous databases

PROiP38815.

Family and domain databases

Gene3Di3.30.1520.10. 1 hit.
InterProiIPR001683. Phox.
[Graphical view]
PfamiPF00787. PX. 1 hit.
[Graphical view]
SMARTiSM00312. PX. 1 hit.
[Graphical view]
SUPFAMiSSF64268. SSF64268. 1 hit.
PROSITEiPS50195. PX. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  2. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  3. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  4. "All phox homology (PX) domains from Saccharomyces cerevisiae specifically recognize phosphatidylinositol 3-phosphate."
    Yu J.W., Lemmon M.A.
    J. Biol. Chem. 276:44179-44184(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: DOMAIN.
  5. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
  6. "The phox homology (PX) domain protein interaction network in yeast."
    Vollert C.S., Uetz P.
    Mol. Cell. Proteomics 3:1053-1064(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH RBD2; YIF1; YIP1 AND YIP4; PHOSPHATIDYLINOSITOL 3-PHOSPHATE-BINDING, MUTAGENESIS OF TYR-123.
  7. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
    Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
    Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-65 AND SER-66, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiYPT35_YEAST
AccessioniPrimary (citable) accession number: P38815
Secondary accession number(s): D3DL55
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: February 1, 1995
Last modified: July 6, 2016
This is version 117 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome VIII
    Yeast (Saccharomyces cerevisiae) chromosome VIII: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.