P38813 (BIG1_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Protein BIG1

Alternative name(s):
Bad in glucose protein 1

Gene names

Name:	BIG1
Ordered Locus Names:	YHR101C

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	335 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Required for normal beta-1,6-glucan synthesis. Ref.3 Ref.4
Subcellular location	Endoplasmic reticulum membrane; Single-pass type I membrane protein Ref.4 Ref.5.
Post-translational modification	N-glycosylated. Ref.4
Miscellaneous	Present with 3460 molecules/cell in log phase SD medium. Ref.6
Sequence similarities	Belongs to the BIG1 family.

Ontologies

Keywords
Biological process	Cell wall biogenesis/degradation
Cellular component	Endoplasmic reticulum Membrane
Domain	Signal Transmembrane Transmembrane helix
PTM	Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	cellular cell wall organization Inferred from electronic annotation. Source: UniProtKB-KW fungal-type cell wall biogenesis Inferred from genetic interaction Ref.4. Source: SGD
Cellular component	integral to endoplasmic reticulum membrane Inferred from direct assay Ref.4. Source: SGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 17	17	Potential
	Chain	18 – 335	318	Protein BIG1		PRO_0000014327
Regions
	Topological domain	20 – 275	256	Lumenal Potential
	Transmembrane	276 – 296	21	Helical; Potential
	Topological domain	297 – 335	39	Cytoplasmic Potential
Amino acid modifications
	Glycosylation	24	1	N-linked (GlcNAc...) Potential
	Glycosylation	144	1	N-linked (GlcNAc...) Potential

Sequences

	Sequence		Length	Mass (Da)
	P38813 [UniParc]. Last modified February 1, 1995. Version 1. Checksum: BA2D68E3A7B8884C	FASTA	335	39,069
	10 20 30 40 50 60 MQTVLKYLLL IMCGSFCASE ELQNQTNVPA IFFSYKLTPG ILKYQEDYDR AVTLPRDTFI 70 80 90 100 110 120 EAAEKFLGVC NADTYVFINQ PGLRKLDFLE FETEFVSLQR YIRRSSTAIK FEKVDLLPQD 130 140 150 160 170 180 LYYDLAEFVK EYCNVDQVLN LRGNNTEDFQ PFIDSEKRVI IIEYPKLPED TNERKEAFRH 190 200 210 220 230 240 YDKYLRTILA QIPSPEQNVI YTSLNPGTTL AHESIIPIQI FPDIFDIKSR VGEVEQNNRV 250 260 270 280 290 300 LDVPRLSFND YTPRFSEPPS EYVSIFDSQL IENNRGLLQL IFTILVGYIL IQFFFTKKTI 310 320 330 VDEKITNKKD NVKQTSPQLL KKVQEIQKKP SQQVS

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Complete nucleotide sequence of Saccharomyces cerevisiae chromosome VIII." Johnston M., Andrews S., Brinkman R., Cooper J., Ding H., Dover J., Du Z., Favello A., Fulton L., Gattung S., Geisel C., Kirsten J., Kucaba T., Hillier L.W., Jier M., Johnston L., Langston Y., Latreille P. , Louis E.J., Macri C., Mardis E., Menezes S., Mouser L., Nhan M., Rifkin L., Riles L., St Peter H., Trevaskis E., Vaughan K., Vignati D., Wilcox L., Wohldman P., Waterston R., Wilson R., Vaudin M. Science 265:2077-2082(1994) [PubMed: 8091229] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[2]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[3]	"Cell wall integrity modulates RHO1 activity via the exchange factor ROM2." Bickle M., Delley P.-A., Schmidt A., Hall M.N. EMBO J. 17:2235-2245(1998) [PubMed: 9545237] [Abstract] Cited for: FUNCTION.
[4]	"Saccharomyces cerevisiae Big1p, a putative endoplasmic reticulum membrane protein required for normal levels of cell wall beta-1,6-glucan." Azuma M., Levinson J.N., Page N., Bussey H. Yeast 19:783-793(2002) [PubMed: 12112232] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION, GLYCOSYLATION.
[5]	"Global analysis of protein localization in budding yeast." Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K. Nature 425:686-691(2003) [PubMed: 14562095] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[6]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U00059 Genomic DNA. Translation: AAB68861.1. BK006934 Genomic DNA. Translation: DAA06795.1.
PIR	S48965.
RefSeq	NP_011969.1. NM_001179231.1.
3D structure databases
ProteinModelPortal	P38813.
ModBase	Search...
Protein-protein interaction databases
IntAct	P38813. 1 interaction.
STRING	P38813.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YHR101C; YHR101C; YHR101C.
GeneID	856501.
KEGG	sce:YHR101C.
NMPDR	fig|4932.3.peg.3130.
Organism-specific databases
CYGD	YHR101c.
SGD	S000001143. BIG1.
Phylogenomic databases
eggNOG	fuNOG10112.
HOGENOM	HBG203773.
OMA	KDEIHLF.
OrthoDB	EOG44F9KM.
Gene expression databases
ArrayExpress	P38813.
Genevestigator	P38813.
GermOnline	YHR101C. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR024722. BIG/ATPase_V1_suS1. [Graphical view]
Pfam	PF05827. ATP-synt_S1. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	982221.

Entry information

Entry name

BIG1_YEAST

Accession

Primary (citable) accession number: P38813
Secondary accession number(s): D3DL51

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1995
Last sequence update:	February 1, 1995
Last modified:	December 14, 2011
This is version 71 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program