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P38812 (GEP4_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 95. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (5) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Phosphatidylglycerophosphatase GEP4, mitochondrial

EC=3.1.3.27
Alternative name(s):
Genetic interactor of prohibitins 4
PGP phosphatase GEP4
Gene names
Name:GEP4
Ordered Locus Names:YHR100C
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length185 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Phosphatidylglycerophosphatase involved in the biosynthesis of cardiolipin (CL), a unique dimeric phosphoglycerolipid predominantly present in mitochondrial membranes and which has important functions for cellular energy metabolism, mitochondrial dynamics and the initiation of apoptotic pathways. Required for the stability of respiratory chain supercomplexes and for growth at elevated temperature, in presence of ethidium bromide or in absence of prohibitins. Ref.5 Ref.6

Catalytic activity

Phosphatidylglycerophosphate + H2O = phosphatidylglycerol + phosphate.

Pathway

Phospholipid metabolism; phosphatidylglycerol biosynthesis; phosphatidylglycerol from CDP-diacylglycerol: step 2/2.

Subcellular location

Mitochondrion inner membrane; Peripheral membrane protein; Matrix side Ref.4 Ref.6.

Sequence similarities

Belongs to the GEP4 family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 185185Phosphatidylglycerophosphatase GEP4, mitochondrial
PRO_0000202908

Regions

Motif45 – 495Phosphoryl acceptor

Experimental info

Mutagenesis451D → N: Abolishes phosphatase activity and impairs cardiolipin biosynthesis. Ref.6
Mutagenesis471D → N: Abolishes phosphatase activity and impairs cardiolipin biosynthesis. Ref.6

Sequences

Sequence LengthMass (Da)Tools
P38812 [UniParc].

Last modified February 1, 1995. Version 1.
Checksum: 1E3D8F936C2EF7EC

FASTA18520,945
        10         20         30         40         50         60 
MNISGTLNTL RLLYNPSLCK PSLVVPTFND LPIPIHDSIK AVVLDKDNCI AFPHDDKIWP 

        70         80         90        100        110        120 
DYLQHWETLR SKYSNKALLI VSNTAGSNSD KDYSQAKLLE DKTGIPVLRH STKKPGCHNE 

       130        140        150        160        170        180 
ILDYFYRNKT ITNPKEVAVV GDRLFTDILM ANLMGSYGVW IRDGVKVSAN PLSKFEKKLY 


NFLGF 

« Hide

References

« Hide 'large scale' references
[1]"Complete nucleotide sequence of Saccharomyces cerevisiae chromosome VIII."
Johnston M., Andrews S., Brinkman R., Cooper J., Ding H., Dover J., Du Z., Favello A., Fulton L., Gattung S., Geisel C., Kirsten J., Kucaba T., Hillier L.W., Jier M., Johnston L., Langston Y., Latreille P. expand/collapse author list , Louis E.J., Macri C., Mardis E., Menezes S., Mouser L., Nhan M., Rifkin L., Riles L., St Peter H., Trevaskis E., Vaughan K., Vignati D., Wilcox L., Wohldman P., Waterston R., Wilson R., Vaudin M.
Science 265:2077-2082(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[2]"The reference genome sequence of Saccharomyces cerevisiae: Then and now."
Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R., Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S., Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.
G3 (Bethesda) 4:389-398(2014) [PubMed] [Europe PMC] [Abstract]
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[3]"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae."
Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. expand/collapse author list , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J.
Genome Res. 17:536-543(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[4]"Toward the complete yeast mitochondrial proteome: multidimensional separation techniques for mitochondrial proteomics."
Reinders J., Zahedi R.P., Pfanner N., Meisinger C., Sickmann A.
J. Proteome Res. 5:1543-1554(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[5]"The genetic interactome of prohibitins: coordinated control of cardiolipin and phosphatidylethanolamine by conserved regulators in mitochondria."
Osman C., Haag M., Potting C., Rodenfels J., Dip P.V., Wieland F.T., Brugger B., Westermann B., Langer T.
J. Cell Biol. 184:583-596(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[6]"A mitochondrial phosphatase required for cardiolipin biosynthesis: the PGP phosphatase Gep4."
Osman C., Haag M., Wieland F.T., Brugger B., Langer T.
EMBO J. 29:1976-1987(2010) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, SUBCELLULAR LOCATION, MUTAGENESIS OF ASP-45 AND ASP-47.
[7]"N-terminal acetylome analyses and functional insights of the N-terminal acetyltransferase NatB."
Van Damme P., Lasa M., Polevoda B., Gazquez C., Elosegui-Artola A., Kim D.S., De Juan-Pardo E., Demeyer K., Hole K., Larrea E., Timmerman E., Prieto J., Arnesen T., Sherman F., Gevaert K., Aldabe R.
Proc. Natl. Acad. Sci. U.S.A. 109:12449-12454(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U00059 Genomic DNA. Translation: AAB68862.1.
AY692650 Genomic DNA. Translation: AAT92669.1.
BK006934 Genomic DNA. Translation: DAA06794.1.
PIRS48943.
RefSeqNP_011968.1. NM_001179230.1.

3D structure databases

ProteinModelPortalP38812.
SMRP38812. Positions 35-86, 129-169.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid36533. 45 interactions.
DIPDIP-1343N.
IntActP38812. 1 interaction.
MINTMINT-391100.
STRING4932.YHR100C.

Proteomic databases

MaxQBP38812.
PaxDbP38812.
PeptideAtlasP38812.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYHR100C; YHR100C; YHR100C.
GeneID856500.
KEGGsce:YHR100C.

Organism-specific databases

CYGDYHR100c.
SGDS000001142. YHR100C.

Phylogenomic databases

eggNOGCOG1011.
HOGENOMHOG000190506.
KOK07025.
OMAGSYGVWI.
OrthoDBEOG7C5MKN.

Enzyme and pathway databases

BioCycYEAST:G3O-31145-MONOMER.
UniPathwayUPA00084; UER00504.

Gene expression databases

GenevestigatorP38812.

Family and domain databases

Gene3D3.40.50.1000. 1 hit.
InterProIPR023214. HAD-like_dom.
IPR010021. HAD_ppase_YqeG-like.
IPR027706. PGP_Pase.
[Graphical view]
PfamPF09419. PGP_phosphatase. 1 hit.
[Graphical view]
SUPFAMSSF56784. SSF56784. 1 hit.
TIGRFAMsTIGR01668. YqeG_hyp_ppase. 1 hit.
ProtoNetSearch...

Other

NextBio982218.

Entry information

Entry nameGEP4_YEAST
AccessionPrimary (citable) accession number: P38812
Secondary accession number(s): D3DL50
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: February 1, 1995
Last modified: June 11, 2014
This is version 95 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome VIII

Yeast (Saccharomyces cerevisiae) chromosome VIII: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

Uncharacterized protein families (UPF)

List of uncharacterized protein family (UPF) entries

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways