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P38812

- GEP4_YEAST

UniProt

P38812 - GEP4_YEAST

Protein

Phosphatidylglycerophosphatase GEP4, mitochondrial

Gene

GEP4

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 97 (01 Oct 2014)
      Sequence version 1 (01 Feb 1995)
      Previous versions | rss
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    Functioni

    Phosphatidylglycerophosphatase involved in the biosynthesis of cardiolipin (CL), a unique dimeric phosphoglycerolipid predominantly present in mitochondrial membranes and which has important functions for cellular energy metabolism, mitochondrial dynamics and the initiation of apoptotic pathways. Required for the stability of respiratory chain supercomplexes and for growth at elevated temperature, in presence of ethidium bromide or in absence of prohibitins.2 Publications

    Catalytic activityi

    Phosphatidylglycerophosphate + H2O = phosphatidylglycerol + phosphate.

    Pathwayi

    GO - Molecular functioni

    1. phosphatidylglycerophosphatase activity Source: SGD

    GO - Biological processi

    1. cardiolipin biosynthetic process Source: SGD
    2. phosphorylated carbohydrate dephosphorylation Source: SGD

    Keywords - Molecular functioni

    Hydrolase

    Keywords - Biological processi

    Lipid biosynthesis, Lipid metabolism, Phospholipid biosynthesis, Phospholipid metabolism

    Enzyme and pathway databases

    BioCyciYEAST:G3O-31145-MONOMER.
    UniPathwayiUPA00084; UER00504.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Phosphatidylglycerophosphatase GEP4, mitochondrial (EC:3.1.3.27)
    Alternative name(s):
    Genetic interactor of prohibitins 4
    PGP phosphatase GEP4
    Gene namesi
    Name:GEP4
    Ordered Locus Names:YHR100C
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome VIII

    Organism-specific databases

    CYGDiYHR100c.
    SGDiS000001142. GEP4.

    Subcellular locationi

    Mitochondrion inner membrane 2 Publications; Peripheral membrane protein 2 Publications; Matrix side 2 Publications

    GO - Cellular componenti

    1. extrinsic component of mitochondrial inner membrane Source: UniProtKB
    2. mitochondrial matrix Source: SGD

    Keywords - Cellular componenti

    Membrane, Mitochondrion, Mitochondrion inner membrane

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi45 – 451D → N: Abolishes phosphatase activity and impairs cardiolipin biosynthesis. 1 Publication
    Mutagenesisi47 – 471D → N: Abolishes phosphatase activity and impairs cardiolipin biosynthesis. 1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 185185Phosphatidylglycerophosphatase GEP4, mitochondrialPRO_0000202908Add
    BLAST

    Proteomic databases

    MaxQBiP38812.
    PaxDbiP38812.
    PeptideAtlasiP38812.

    Expressioni

    Gene expression databases

    GenevestigatoriP38812.

    Interactioni

    Protein-protein interaction databases

    BioGridi36533. 45 interactions.
    DIPiDIP-1343N.
    IntActiP38812. 1 interaction.
    MINTiMINT-391100.
    STRINGi4932.YHR100C.

    Structurei

    3D structure databases

    ProteinModelPortaliP38812.
    SMRiP38812. Positions 35-86, 129-169.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi45 – 495Phosphoryl acceptor

    Sequence similaritiesi

    Belongs to the GEP4 family.Curated

    Phylogenomic databases

    eggNOGiCOG1011.
    HOGENOMiHOG000190506.
    KOiK07025.
    OMAiGSYGVWI.
    OrthoDBiEOG7C5MKN.

    Family and domain databases

    Gene3Di3.40.50.1000. 1 hit.
    InterProiIPR023214. HAD-like_dom.
    IPR010021. HAD_ppase_YqeG-like.
    IPR027706. PGP_Pase.
    [Graphical view]
    PfamiPF09419. PGP_phosphatase. 1 hit.
    [Graphical view]
    SUPFAMiSSF56784. SSF56784. 1 hit.
    TIGRFAMsiTIGR01668. YqeG_hyp_ppase. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    P38812-1 [UniParc]FASTAAdd to Basket

    « Hide

    MNISGTLNTL RLLYNPSLCK PSLVVPTFND LPIPIHDSIK AVVLDKDNCI    50
    AFPHDDKIWP DYLQHWETLR SKYSNKALLI VSNTAGSNSD KDYSQAKLLE 100
    DKTGIPVLRH STKKPGCHNE ILDYFYRNKT ITNPKEVAVV GDRLFTDILM 150
    ANLMGSYGVW IRDGVKVSAN PLSKFEKKLY NFLGF 185
    Length:185
    Mass (Da):20,945
    Last modified:February 1, 1995 - v1
    Checksum:i1E3D8F936C2EF7EC
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U00059 Genomic DNA. Translation: AAB68862.1.
    AY692650 Genomic DNA. Translation: AAT92669.1.
    BK006934 Genomic DNA. Translation: DAA06794.1.
    PIRiS48943.
    RefSeqiNP_011968.1. NM_001179230.1.

    Genome annotation databases

    EnsemblFungiiYHR100C; YHR100C; YHR100C.
    GeneIDi856500.
    KEGGisce:YHR100C.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U00059 Genomic DNA. Translation: AAB68862.1 .
    AY692650 Genomic DNA. Translation: AAT92669.1 .
    BK006934 Genomic DNA. Translation: DAA06794.1 .
    PIRi S48943.
    RefSeqi NP_011968.1. NM_001179230.1.

    3D structure databases

    ProteinModelPortali P38812.
    SMRi P38812. Positions 35-86, 129-169.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 36533. 45 interactions.
    DIPi DIP-1343N.
    IntActi P38812. 1 interaction.
    MINTi MINT-391100.
    STRINGi 4932.YHR100C.

    Proteomic databases

    MaxQBi P38812.
    PaxDbi P38812.
    PeptideAtlasi P38812.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YHR100C ; YHR100C ; YHR100C .
    GeneIDi 856500.
    KEGGi sce:YHR100C.

    Organism-specific databases

    CYGDi YHR100c.
    SGDi S000001142. GEP4.

    Phylogenomic databases

    eggNOGi COG1011.
    HOGENOMi HOG000190506.
    KOi K07025.
    OMAi GSYGVWI.
    OrthoDBi EOG7C5MKN.

    Enzyme and pathway databases

    UniPathwayi UPA00084 ; UER00504 .
    BioCyci YEAST:G3O-31145-MONOMER.

    Miscellaneous databases

    NextBioi 982218.

    Gene expression databases

    Genevestigatori P38812.

    Family and domain databases

    Gene3Di 3.40.50.1000. 1 hit.
    InterProi IPR023214. HAD-like_dom.
    IPR010021. HAD_ppase_YqeG-like.
    IPR027706. PGP_Pase.
    [Graphical view ]
    Pfami PF09419. PGP_phosphatase. 1 hit.
    [Graphical view ]
    SUPFAMi SSF56784. SSF56784. 1 hit.
    TIGRFAMsi TIGR01668. YqeG_hyp_ppase. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    2. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    3. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    4. "Toward the complete yeast mitochondrial proteome: multidimensional separation techniques for mitochondrial proteomics."
      Reinders J., Zahedi R.P., Pfanner N., Meisinger C., Sickmann A.
      J. Proteome Res. 5:1543-1554(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
    5. "The genetic interactome of prohibitins: coordinated control of cardiolipin and phosphatidylethanolamine by conserved regulators in mitochondria."
      Osman C., Haag M., Potting C., Rodenfels J., Dip P.V., Wieland F.T., Brugger B., Westermann B., Langer T.
      J. Cell Biol. 184:583-596(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    6. "A mitochondrial phosphatase required for cardiolipin biosynthesis: the PGP phosphatase Gep4."
      Osman C., Haag M., Wieland F.T., Brugger B., Langer T.
      EMBO J. 29:1976-1987(2010) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, SUBCELLULAR LOCATION, MUTAGENESIS OF ASP-45 AND ASP-47.
    7. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiGEP4_YEAST
    AccessioniPrimary (citable) accession number: P38812
    Secondary accession number(s): D3DL50
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 1, 1995
    Last sequence update: February 1, 1995
    Last modified: October 1, 2014
    This is version 97 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families
    3. Uncharacterized protein families (UPF)
      List of uncharacterized protein family (UPF) entries
    4. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    5. Yeast chromosome VIII
      Yeast (Saccharomyces cerevisiae) chromosome VIII: entries and gene names

    External Data

    Dasty 3