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P38812

- GEP4_YEAST

UniProt

P38812 - GEP4_YEAST

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Protein

Phosphatidylglycerophosphatase GEP4, mitochondrial

Gene

GEP4

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli

Functioni

Phosphatidylglycerophosphatase involved in the biosynthesis of cardiolipin (CL), a unique dimeric phosphoglycerolipid predominantly present in mitochondrial membranes and which has important functions for cellular energy metabolism, mitochondrial dynamics and the initiation of apoptotic pathways. Required for the stability of respiratory chain supercomplexes and for growth at elevated temperature, in presence of ethidium bromide or in absence of prohibitins.2 Publications

Catalytic activityi

Phosphatidylglycerophosphate + H2O = phosphatidylglycerol + phosphate.

Pathwayi

GO - Molecular functioni

  1. phosphatidylglycerophosphatase activity Source: SGD

GO - Biological processi

  1. cardiolipin biosynthetic process Source: SGD
  2. phosphorylated carbohydrate dephosphorylation Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Lipid biosynthesis, Lipid metabolism, Phospholipid biosynthesis, Phospholipid metabolism

Enzyme and pathway databases

BioCyciYEAST:G3O-31145-MONOMER.
UniPathwayiUPA00084; UER00504.

Names & Taxonomyi

Protein namesi
Recommended name:
Phosphatidylglycerophosphatase GEP4, mitochondrial (EC:3.1.3.27)
Alternative name(s):
Genetic interactor of prohibitins 4
PGP phosphatase GEP4
Gene namesi
Name:GEP4
Ordered Locus Names:YHR100C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome VIII

Organism-specific databases

CYGDiYHR100c.
SGDiS000001142. GEP4.

Subcellular locationi

Mitochondrion inner membrane 2 Publications; Peripheral membrane protein 2 Publications; Matrix side 2 Publications

GO - Cellular componenti

  1. extrinsic component of mitochondrial inner membrane Source: UniProtKB
  2. mitochondrial matrix Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Membrane, Mitochondrion, Mitochondrion inner membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi45 – 451D → N: Abolishes phosphatase activity and impairs cardiolipin biosynthesis. 1 Publication
Mutagenesisi47 – 471D → N: Abolishes phosphatase activity and impairs cardiolipin biosynthesis. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 185185Phosphatidylglycerophosphatase GEP4, mitochondrialPRO_0000202908Add
BLAST

Proteomic databases

MaxQBiP38812.
PaxDbiP38812.
PeptideAtlasiP38812.

Expressioni

Gene expression databases

GenevestigatoriP38812.

Interactioni

Protein-protein interaction databases

BioGridi36533. 46 interactions.
DIPiDIP-1343N.
IntActiP38812. 1 interaction.
MINTiMINT-391100.
STRINGi4932.YHR100C.

Structurei

3D structure databases

ProteinModelPortaliP38812.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi45 – 495Phosphoryl acceptor

Sequence similaritiesi

Belongs to the GEP4 family.Curated

Phylogenomic databases

eggNOGiCOG1011.
HOGENOMiHOG000190506.
InParanoidiP38812.
KOiK01094.
OMAiGSYGVWI.
OrthoDBiEOG7C5MKN.

Family and domain databases

Gene3Di3.40.50.1000. 1 hit.
InterProiIPR023214. HAD-like_dom.
IPR010021. HAD_ppase_YqeG-like.
IPR027706. PGP_Pase.
[Graphical view]
PfamiPF09419. PGP_phosphatase. 1 hit.
[Graphical view]
SUPFAMiSSF56784. SSF56784. 1 hit.
TIGRFAMsiTIGR01668. YqeG_hyp_ppase. 1 hit.

Sequencei

Sequence statusi: Complete.

P38812-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MNISGTLNTL RLLYNPSLCK PSLVVPTFND LPIPIHDSIK AVVLDKDNCI
60 70 80 90 100
AFPHDDKIWP DYLQHWETLR SKYSNKALLI VSNTAGSNSD KDYSQAKLLE
110 120 130 140 150
DKTGIPVLRH STKKPGCHNE ILDYFYRNKT ITNPKEVAVV GDRLFTDILM
160 170 180
ANLMGSYGVW IRDGVKVSAN PLSKFEKKLY NFLGF
Length:185
Mass (Da):20,945
Last modified:February 1, 1995 - v1
Checksum:i1E3D8F936C2EF7EC
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00059 Genomic DNA. Translation: AAB68862.1.
AY692650 Genomic DNA. Translation: AAT92669.1.
BK006934 Genomic DNA. Translation: DAA06794.1.
PIRiS48943.
RefSeqiNP_011968.1. NM_001179230.1.

Genome annotation databases

EnsemblFungiiYHR100C; YHR100C; YHR100C.
GeneIDi856500.
KEGGisce:YHR100C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00059 Genomic DNA. Translation: AAB68862.1 .
AY692650 Genomic DNA. Translation: AAT92669.1 .
BK006934 Genomic DNA. Translation: DAA06794.1 .
PIRi S48943.
RefSeqi NP_011968.1. NM_001179230.1.

3D structure databases

ProteinModelPortali P38812.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 36533. 46 interactions.
DIPi DIP-1343N.
IntActi P38812. 1 interaction.
MINTi MINT-391100.
STRINGi 4932.YHR100C.

Proteomic databases

MaxQBi P38812.
PaxDbi P38812.
PeptideAtlasi P38812.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YHR100C ; YHR100C ; YHR100C .
GeneIDi 856500.
KEGGi sce:YHR100C.

Organism-specific databases

CYGDi YHR100c.
SGDi S000001142. GEP4.

Phylogenomic databases

eggNOGi COG1011.
HOGENOMi HOG000190506.
InParanoidi P38812.
KOi K01094.
OMAi GSYGVWI.
OrthoDBi EOG7C5MKN.

Enzyme and pathway databases

UniPathwayi UPA00084 ; UER00504 .
BioCyci YEAST:G3O-31145-MONOMER.

Miscellaneous databases

NextBioi 982218.

Gene expression databases

Genevestigatori P38812.

Family and domain databases

Gene3Di 3.40.50.1000. 1 hit.
InterProi IPR023214. HAD-like_dom.
IPR010021. HAD_ppase_YqeG-like.
IPR027706. PGP_Pase.
[Graphical view ]
Pfami PF09419. PGP_phosphatase. 1 hit.
[Graphical view ]
SUPFAMi SSF56784. SSF56784. 1 hit.
TIGRFAMsi TIGR01668. YqeG_hyp_ppase. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  2. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  3. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  4. "Toward the complete yeast mitochondrial proteome: multidimensional separation techniques for mitochondrial proteomics."
    Reinders J., Zahedi R.P., Pfanner N., Meisinger C., Sickmann A.
    J. Proteome Res. 5:1543-1554(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
  5. "The genetic interactome of prohibitins: coordinated control of cardiolipin and phosphatidylethanolamine by conserved regulators in mitochondria."
    Osman C., Haag M., Potting C., Rodenfels J., Dip P.V., Wieland F.T., Brugger B., Westermann B., Langer T.
    J. Cell Biol. 184:583-596(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  6. "A mitochondrial phosphatase required for cardiolipin biosynthesis: the PGP phosphatase Gep4."
    Osman C., Haag M., Wieland F.T., Brugger B., Langer T.
    EMBO J. 29:1976-1987(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION, MUTAGENESIS OF ASP-45 AND ASP-47.
  7. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiGEP4_YEAST
AccessioniPrimary (citable) accession number: P38812
Secondary accession number(s): D3DL50
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: February 1, 1995
Last modified: November 26, 2014
This is version 99 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families
  3. Uncharacterized protein families (UPF)
    List of uncharacterized protein family (UPF) entries
  4. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  5. Yeast chromosome VIII
    Yeast (Saccharomyces cerevisiae) chromosome VIII: entries and gene names

External Data

Dasty 3