P38812 (GEP4_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Phosphatidylglycerophosphatase GEP4, mitochondrial
EC=3.1.3.27

Alternative name(s):
Genetic interactor of prohibitins 4
PGP phosphatase GEP4

Gene names

Name:	GEP4
Ordered Locus Names:	YHR100C

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › Saccharomyces cerevisiae

Protein attributes

Sequence length	185 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Phosphatidylglycerophosphatase involved in the biosynthesis of cardiolipin (CL), a unique dimeric phosphoglycerolipid predominantly present in mitochondrial membranes and which has important functions for cellular energy metabolism, mitochondrial dynamics and the initiation of apoptotic pathways. Required for the stability of respiratory chain supercomplexes and for growth at elevated temperature, in presence of ethidium bromide or in absence of prohibitins. Ref.5 Ref.6
Catalytic activity	Phosphatidylglycerophosphate + H2O = phosphatidylglycerol + phosphate.
Pathway	Phospholipid metabolism; phosphatidylglycerol biosynthesis; phosphatidylglycerol from CDP-diacylglycerol: step 2/2.
Subcellular location	Mitochondrion inner membrane; Peripheral membrane protein; Matrix side Ref.4 Ref.6.
Sequence similarities	Belongs to the GEP4 family.

Ontologies

Keywords
Biological process	Lipid biosynthesis Lipid metabolism Phospholipid biosynthesis Phospholipid metabolism
Cellular component	Membrane Mitochondrion Mitochondrion inner membrane
Molecular function	Hydrolase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	cardiolipin biosynthetic process Inferred from mutant phenotype Ref.6. Source: SGD phosphorylated carbohydrate dephosphorylation Inferred from mutant phenotype Ref.6. Source: SGD
Cellular_component	extrinsic to mitochondrial inner membrane Inferred from direct assay Ref.6. Source: UniProtKB mitochondrial matrix Inferred from direct assay Ref.6. Source: SGD
Molecular_function	phosphatidylglycerophosphatase activity Inferred from mutant phenotype Ref.6. Source: SGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 185	185	Phosphatidylglycerophosphatase GEP4, mitochondrial		PRO_0000202908
Regions
	Motif	45 – 49	5	Phosphoryl acceptor
Experimental info
	Mutagenesis	45	1	D → N: Abolishes phosphatase activity and impairs cardiolipin biosynthesis. Ref.6
	Mutagenesis	47	1	D → N: Abolishes phosphatase activity and impairs cardiolipin biosynthesis. Ref.6

Sequences

	Sequence		Length	Mass (Da)
	P38812 [UniParc]. Last modified February 1, 1995. Version 1. Checksum: 1E3D8F936C2EF7EC	FASTA	185	20,945
	10 20 30 40 50 60 MNISGTLNTL RLLYNPSLCK PSLVVPTFND LPIPIHDSIK AVVLDKDNCI AFPHDDKIWP 70 80 90 100 110 120 DYLQHWETLR SKYSNKALLI VSNTAGSNSD KDYSQAKLLE DKTGIPVLRH STKKPGCHNE 130 140 150 160 170 180 ILDYFYRNKT ITNPKEVAVV GDRLFTDILM ANLMGSYGVW IRDGVKVSAN PLSKFEKKLY NFLGF

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Complete nucleotide sequence of Saccharomyces cerevisiae chromosome VIII." Johnston M., Andrews S., Brinkman R., Cooper J., Ding H., Dover J., Du Z., Favello A., Fulton L., Gattung S., Geisel C., Kirsten J., Kucaba T., Hillier L.W., Jier M., Johnston L., Langston Y., Latreille P. , Louis E.J., Macri C., Mardis E., Menezes S., Mouser L., Nhan M., Rifkin L., Riles L., St Peter H., Trevaskis E., Vaughan K., Vignati D., Wilcox L., Wohldman P., Waterston R., Wilson R., Vaudin M. Science 265:2077-2082(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[2]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[3]	"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae." Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J. Genome Res. 17:536-543(2007) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[4]	"Toward the complete yeast mitochondrial proteome: multidimensional separation techniques for mitochondrial proteomics." Reinders J., Zahedi R.P., Pfanner N., Meisinger C., Sickmann A. J. Proteome Res. 5:1543-1554(2006) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS], IDENTIFICATION BY MASS SPECTROMETRY.
[5]	"The genetic interactome of prohibitins: coordinated control of cardiolipin and phosphatidylethanolamine by conserved regulators in mitochondria." Osman C., Haag M., Potting C., Rodenfels J., Dip P.V., Wieland F.T., Brugger B., Westermann B., Langer T. J. Cell Biol. 184:583-596(2009) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
[6]	"A mitochondrial phosphatase required for cardiolipin biosynthesis: the PGP phosphatase Gep4." Osman C., Haag M., Wieland F.T., Brugger B., Langer T. EMBO J. 29:1976-1987(2010) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION, MUTAGENESIS OF ASP-45 AND ASP-47.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U00059 Genomic DNA. Translation: AAB68862.1. AY692650 Genomic DNA. Translation: AAT92669.1. BK006934 Genomic DNA. Translation: DAA06794.1.
PIR	S48943.
RefSeq	NP_011968.1. NM_001179230.1.
3D structure databases
ProteinModelPortal	P38812.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-1343N.
IntAct	P38812. 1 interaction.
MINT	MINT-391100.
STRING	4932.YHR100C.
Proteomic databases
PaxDb	P38812.
PeptideAtlas	P38812.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YHR100C; YHR100C; YHR100C.
GeneID	856500.
KEGG	sce:YHR100C.
Organism-specific databases
CYGD	YHR100c.
SGD	S000001142. YHR100C.
Phylogenomic databases
eggNOG	COG1011.
HOGENOM	HOG000190506.
KO	K07025.
OMA	LDKDNCI.
OrthoDB	EOG4BGD5F.
Enzyme and pathway databases
UniPathway	UPA00084; UER00504.
Gene expression databases
Genevestigator	P38812.
GermOnline	YHR100C. Saccharomyces cerevisiae.
Family and domain databases
Gene3D	3.40.50.1000. 1 hit.
InterPro	IPR023214. HAD-like_dom. IPR010021. HAD-SF_ppase_IIIA. [Graphical view]
Pfam	PF09419. PGP_phosphatase. 1 hit. [Graphical view]
SUPFAM	SSF56784. HAD-like_dom. 1 hit.
TIGRFAMs	TIGR01668. YqeG_hyp_ppase. 1 hit.
ProtoNet	Search...
Other
NextBio	982218.

Entry information

Entry name

GEP4_YEAST

Accession

Primary (citable) accession number: P38812
Secondary accession number(s): D3DL50

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1995
Last sequence update:	February 1, 1995
Last modified:	May 1, 2013
This is version 84 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program