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Protein

Phosphatidylglycerophosphatase GEP4, mitochondrial

Gene

GEP4

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Phosphatidylglycerophosphatase involved in the biosynthesis of cardiolipin (CL), a unique dimeric phosphoglycerolipid predominantly present in mitochondrial membranes and which has important functions for cellular energy metabolism, mitochondrial dynamics and the initiation of apoptotic pathways. Required for the stability of respiratory chain supercomplexes and for growth at elevated temperature, in presence of ethidium bromide or in absence of prohibitins.2 Publications

Catalytic activityi

Phosphatidylglycerophosphate + H2O = phosphatidylglycerol + phosphate.

Pathway:iphosphatidylglycerol biosynthesis

This protein is involved in step 2 of the subpathway that synthesizes phosphatidylglycerol from CDP-diacylglycerol.
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. CDP-diacylglycerol--glycerol-3-phosphate 3-phosphatidyltransferase (PGS1)
  2. Phosphatidylglycerophosphatase GEP4, mitochondrial (GEP4)
This subpathway is part of the pathway phosphatidylglycerol biosynthesis, which is itself part of Phospholipid metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes phosphatidylglycerol from CDP-diacylglycerol, the pathway phosphatidylglycerol biosynthesis and in Phospholipid metabolism.

GO - Molecular functioni

  • phosphatidylglycerophosphatase activity Source: SGD

GO - Biological processi

  • cardiolipin biosynthetic process Source: SGD
  • phosphorylated carbohydrate dephosphorylation Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Lipid biosynthesis, Lipid metabolism, Phospholipid biosynthesis, Phospholipid metabolism

Enzyme and pathway databases

BioCyciYEAST:G3O-31145-MONOMER.
UniPathwayiUPA00084; UER00504.

Names & Taxonomyi

Protein namesi
Recommended name:
Phosphatidylglycerophosphatase GEP4, mitochondrial (EC:3.1.3.27)
Alternative name(s):
Genetic interactor of prohibitins 4
PGP phosphatase GEP4
Gene namesi
Name:GEP4
Ordered Locus Names:YHR100C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311 Componenti: Chromosome VIII

Organism-specific databases

CYGDiYHR100c.
EuPathDBiFungiDB:YHR100C.
SGDiS000001142. GEP4.

Subcellular locationi

GO - Cellular componenti

  • extrinsic component of mitochondrial inner membrane Source: UniProtKB
  • mitochondrial matrix Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Membrane, Mitochondrion, Mitochondrion inner membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi45 – 451D → N: Abolishes phosphatase activity and impairs cardiolipin biosynthesis. 1 Publication
Mutagenesisi47 – 471D → N: Abolishes phosphatase activity and impairs cardiolipin biosynthesis. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 185185Phosphatidylglycerophosphatase GEP4, mitochondrialPRO_0000202908Add
BLAST

Proteomic databases

MaxQBiP38812.
PaxDbiP38812.
PeptideAtlasiP38812.

Interactioni

Protein-protein interaction databases

BioGridi36533. 46 interactions.
DIPiDIP-1343N.
IntActiP38812. 1 interaction.
MINTiMINT-391100.

Structurei

3D structure databases

ProteinModelPortaliP38812.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi45 – 495Phosphoryl acceptor

Sequence similaritiesi

Belongs to the GEP4 family.Curated

Phylogenomic databases

eggNOGiCOG1011.
HOGENOMiHOG000190506.
InParanoidiP38812.
KOiK01094.
OMAiGSYGVWI.
OrthoDBiEOG7C5MKN.

Family and domain databases

Gene3Di3.40.50.1000. 1 hit.
InterProiIPR023214. HAD-like_dom.
IPR010021. HAD_ppase_YqeG-like.
IPR027706. PGP_Pase.
[Graphical view]
PfamiPF09419. PGP_phosphatase. 1 hit.
[Graphical view]
SUPFAMiSSF56784. SSF56784. 1 hit.
TIGRFAMsiTIGR01668. YqeG_hyp_ppase. 1 hit.

Sequencei

Sequence statusi: Complete.

P38812-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNISGTLNTL RLLYNPSLCK PSLVVPTFND LPIPIHDSIK AVVLDKDNCI
60 70 80 90 100
AFPHDDKIWP DYLQHWETLR SKYSNKALLI VSNTAGSNSD KDYSQAKLLE
110 120 130 140 150
DKTGIPVLRH STKKPGCHNE ILDYFYRNKT ITNPKEVAVV GDRLFTDILM
160 170 180
ANLMGSYGVW IRDGVKVSAN PLSKFEKKLY NFLGF
Length:185
Mass (Da):20,945
Last modified:February 1, 1995 - v1
Checksum:i1E3D8F936C2EF7EC
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00059 Genomic DNA. Translation: AAB68862.1.
AY692650 Genomic DNA. Translation: AAT92669.1.
BK006934 Genomic DNA. Translation: DAA06794.1.
PIRiS48943.
RefSeqiNP_011968.1. NM_001179230.1.

Genome annotation databases

EnsemblFungiiYHR100C; YHR100C; YHR100C.
GeneIDi856500.
KEGGisce:YHR100C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00059 Genomic DNA. Translation: AAB68862.1.
AY692650 Genomic DNA. Translation: AAT92669.1.
BK006934 Genomic DNA. Translation: DAA06794.1.
PIRiS48943.
RefSeqiNP_011968.1. NM_001179230.1.

3D structure databases

ProteinModelPortaliP38812.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi36533. 46 interactions.
DIPiDIP-1343N.
IntActiP38812. 1 interaction.
MINTiMINT-391100.

Proteomic databases

MaxQBiP38812.
PaxDbiP38812.
PeptideAtlasiP38812.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYHR100C; YHR100C; YHR100C.
GeneIDi856500.
KEGGisce:YHR100C.

Organism-specific databases

CYGDiYHR100c.
EuPathDBiFungiDB:YHR100C.
SGDiS000001142. GEP4.

Phylogenomic databases

eggNOGiCOG1011.
HOGENOMiHOG000190506.
InParanoidiP38812.
KOiK01094.
OMAiGSYGVWI.
OrthoDBiEOG7C5MKN.

Enzyme and pathway databases

UniPathwayiUPA00084; UER00504.
BioCyciYEAST:G3O-31145-MONOMER.

Miscellaneous databases

NextBioi982218.
PROiP38812.

Family and domain databases

Gene3Di3.40.50.1000. 1 hit.
InterProiIPR023214. HAD-like_dom.
IPR010021. HAD_ppase_YqeG-like.
IPR027706. PGP_Pase.
[Graphical view]
PfamiPF09419. PGP_phosphatase. 1 hit.
[Graphical view]
SUPFAMiSSF56784. SSF56784. 1 hit.
TIGRFAMsiTIGR01668. YqeG_hyp_ppase. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  2. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  3. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  4. "Toward the complete yeast mitochondrial proteome: multidimensional separation techniques for mitochondrial proteomics."
    Reinders J., Zahedi R.P., Pfanner N., Meisinger C., Sickmann A.
    J. Proteome Res. 5:1543-1554(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
  5. "The genetic interactome of prohibitins: coordinated control of cardiolipin and phosphatidylethanolamine by conserved regulators in mitochondria."
    Osman C., Haag M., Potting C., Rodenfels J., Dip P.V., Wieland F.T., Brugger B., Westermann B., Langer T.
    J. Cell Biol. 184:583-596(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  6. "A mitochondrial phosphatase required for cardiolipin biosynthesis: the PGP phosphatase Gep4."
    Osman C., Haag M., Wieland F.T., Brugger B., Langer T.
    EMBO J. 29:1976-1987(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION, MUTAGENESIS OF ASP-45 AND ASP-47.
  7. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiGEP4_YEAST
AccessioniPrimary (citable) accession number: P38812
Secondary accession number(s): D3DL50
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: February 1, 1995
Last modified: July 22, 2015
This is version 103 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families
  3. Uncharacterized protein families (UPF)
    List of uncharacterized protein family (UPF) entries
  4. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  5. Yeast chromosome VIII
    Yeast (Saccharomyces cerevisiae) chromosome VIII: entries and gene names

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.