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Protein

Phosphatidylglycerophosphatase GEP4, mitochondrial

Gene

GEP4

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Phosphatidylglycerophosphatase involved in the biosynthesis of cardiolipin (CL), a unique dimeric phosphoglycerolipid predominantly present in mitochondrial membranes and which has important functions for cellular energy metabolism, mitochondrial dynamics and the initiation of apoptotic pathways. Required for the stability of respiratory chain supercomplexes and for growth at elevated temperature, in presence of ethidium bromide or in absence of prohibitins.2 Publications

Catalytic activityi

Phosphatidylglycerophosphate + H2O = phosphatidylglycerol + phosphate.

Pathwayi: phosphatidylglycerol biosynthesis

This protein is involved in step 2 of the subpathway that synthesizes phosphatidylglycerol from CDP-diacylglycerol.
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. CDP-diacylglycerol--glycerol-3-phosphate 3-phosphatidyltransferase (PGS1)
  2. Phosphatidylglycerophosphatase GEP4, mitochondrial (GEP4)
This subpathway is part of the pathway phosphatidylglycerol biosynthesis, which is itself part of Phospholipid metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes phosphatidylglycerol from CDP-diacylglycerol, the pathway phosphatidylglycerol biosynthesis and in Phospholipid metabolism.

GO - Molecular functioni

  • phosphatidylglycerophosphatase activity Source: SGD

GO - Biological processi

  • cardiolipin biosynthetic process Source: SGD
  • phosphorylated carbohydrate dephosphorylation Source: SGD

Keywordsi

Molecular functionHydrolase
Biological processLipid biosynthesis, Lipid metabolism, Phospholipid biosynthesis, Phospholipid metabolism

Enzyme and pathway databases

BioCyciYEAST:G3O-31145-MONOMER
UniPathwayiUPA00084; UER00504

Chemistry databases

SwissLipidsiSLP:000000061
SLP:000000220

Names & Taxonomyi

Protein namesi
Recommended name:
Phosphatidylglycerophosphatase GEP4, mitochondrial (EC:3.1.3.27)
Alternative name(s):
Genetic interactor of prohibitins 4
PGP phosphatase GEP4
Gene namesi
Name:GEP4
Ordered Locus Names:YHR100C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome VIII

Organism-specific databases

EuPathDBiFungiDB:YHR100C
SGDiS000001142 GEP4

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Membrane, Mitochondrion, Mitochondrion inner membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi45D → N: Abolishes phosphatase activity and impairs cardiolipin biosynthesis. 1 Publication1
Mutagenesisi47D → N: Abolishes phosphatase activity and impairs cardiolipin biosynthesis. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002029081 – 185Phosphatidylglycerophosphatase GEP4, mitochondrialAdd BLAST185

Proteomic databases

MaxQBiP38812
PaxDbiP38812
PRIDEiP38812

Interactioni

Protein-protein interaction databases

BioGridi36533, 47 interactors
DIPiDIP-1343N
IntActiP38812, 1 interactor
MINTiP38812
STRINGi4932.YHR100C

Structurei

3D structure databases

ProteinModelPortaliP38812
SMRiP38812
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi45 – 49Phosphoryl acceptor5

Sequence similaritiesi

Belongs to the GEP4 family.Curated

Phylogenomic databases

HOGENOMiHOG000190506
InParanoidiP38812
KOiK01094
OMAiDKDDCFA
OrthoDBiEOG092C2933

Family and domain databases

Gene3Di3.40.50.1000, 1 hit
InterProiView protein in InterPro
IPR036412 HAD-like_sf
IPR010021 HAD_ppase_YqeG-like
IPR023214 HAD_sf
IPR027706 PGP_Pase
PfamiView protein in Pfam
PF09419 PGP_phosphatase, 1 hit
SUPFAMiSSF56784 SSF56784, 1 hit
TIGRFAMsiTIGR01668 YqeG_hyp_ppase, 1 hit

Sequencei

Sequence statusi: Complete.

P38812-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNISGTLNTL RLLYNPSLCK PSLVVPTFND LPIPIHDSIK AVVLDKDNCI
60 70 80 90 100
AFPHDDKIWP DYLQHWETLR SKYSNKALLI VSNTAGSNSD KDYSQAKLLE
110 120 130 140 150
DKTGIPVLRH STKKPGCHNE ILDYFYRNKT ITNPKEVAVV GDRLFTDILM
160 170 180
ANLMGSYGVW IRDGVKVSAN PLSKFEKKLY NFLGF
Length:185
Mass (Da):20,945
Last modified:February 1, 1995 - v1
Checksum:i1E3D8F936C2EF7EC
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00059 Genomic DNA Translation: AAB68862.1
AY692650 Genomic DNA Translation: AAT92669.1
BK006934 Genomic DNA Translation: DAA06794.1
PIRiS48943
RefSeqiNP_011968.1, NM_001179230.1

Genome annotation databases

EnsemblFungiiYHR100C; YHR100C; YHR100C
GeneIDi856500
KEGGisce:YHR100C

Similar proteinsi

Entry informationi

Entry nameiGEP4_YEAST
AccessioniPrimary (citable) accession number: P38812
Secondary accession number(s): D3DL50
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: February 1, 1995
Last modified: March 28, 2018
This is version 121 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health