Reviewed, UniProtKB/Swiss-Prot P38810 (SFB3_YEAST)

Names and origin

Protein names

Recommended name:
    SED5-binding protein 3
Alternative name(s):
    SEC24-related protein 3
    Lethal with SEC13 protein 1

Gene names

Name:	SFB3
Synonyms:	LST1
Ordered Locus Names:	YHR098C

Organism

Saccharomyces cerevisiae (Baker's yeast) [Complete proteome]

Taxonomic identifier

4932 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	929 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Component of the COPII coat, that covers ER-derived vesicles involved in transport from the endoplasmic reticulum to the Golgi apparatus. COPII acts in the cytoplasm to promote the transport of secretory, plasma membrane, and vacuolar proteins from the endoplasmic reticulum to the Golgi complex.
Subunit structure	COPII is composed of at least five proteins: the SEC23/24 complex, the SEC13/31 complex and SAR1. Binds to SED5.
Subcellular location	Cytoplasm By similarity. Golgi apparatus membrane By similarity. Endoplasmic reticulum membrane By similarity.
Miscellaneous	Present with 12200 molecules/cell in log phase SD medium. Ref.4
Sequence similarities	Belongs to the SEC23/SEC24 family. SEC24 subfamily.

Ontologies

Keywords
Biological process	ER-Golgi transport Protein transport Transport
Cellular component	Cytoplasm Endoplasmic reticulum Golgi apparatus Membrane
PTM	Phosphoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	ER to Golgi vesicle-mediated transport Ref.3 Inferred from genetic interaction. Source: SGD intracellular protein transport Inferred from electronic annotation. Source: InterPro
Cellular component	COPII vesicle coat Ref.3 Inferred from genetic interaction. Source: SGD endoplasmic reticulum membrane Inferred from electronic annotation. Source: UniProtKB-SubCell mating projection tip Inferred from direct assay. Source: SGD peripheral to membrane of membrane fraction Ref.3 Inferred from direct assay. Source: SGD
Molecular function	identical protein binding Inferred from physical interaction. Source: IntAct zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
itself		1	EBI-17012,EBI-17012
CYC1	P00044	1	EBI-17012,EBI-5393
PMP2	P40975	1	EBI-17012,EBI-2043041
RBD2	Q12270	1	EBI-17012,EBI-31471
SEC24	P40482	1	EBI-17012,EBI-16592

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 929	929	SED5-binding protein 3		PRO_0000205152
Regions
	Region	220 – 244	25	Zinc finger-like
Amino acid modifications
	Modified residue	15	1	Phosphoserine Ref.7
	Modified residue	52	1	Phosphoserine Ref.7
	Modified residue	83	1	Phosphoserine Ref.7
	Modified residue	85	1	Phosphoserine Ref.7
	Modified residue	94	1	Phosphoserine Ref.7
	Modified residue	101	1	Phosphoserine Ref.7 Ref.5
	Modified residue	105	1	Phosphoserine Ref.7
	Modified residue	110	1	Phosphoserine Ref.7 Ref.6
	Modified residue	216	1	Phosphothreonine Ref.7

Sequences

	Sequence		Length	Mass (Da)
	P38810-1 [UniParc]. Last modified February 1, 1995. Version 1. Checksum: 6A028AD6B1A2E031	FASTA	929	103,950
	10 20 30 40 50 60 MSQQNILAAS VSALSLDEST VHTGGASSKK SRRPHRAYHN FSSGTVPTLG NSPYTTPQLN 70 80 90 100 110 120 QQDGFQQPQA FTPKQFGGFN NGSGSVMSTP VMVSQERFGA SEASSPYGQS MLDMTAPQPT 130 140 150 160 170 180 SHIVPTQRFE DQAQYLQRSF ETCRDSVPPL PTTQFYCVDQ GSCDPHLMSL SMYNIPESEH 190 200 210 220 230 240 LRAATKLPLG LTIQPFSTLT PNDAEVPTIP LPMDGTPLRC RRCRAYANPK FQFTYDSSVI 250 260 270 280 290 300 CNICRVKMQV PGEHFAPMGP NGQRSDLNEK SELLHGTVDF LVPSIYNAIQ EKELLPLHYV 310 320 330 340 350 360 FLIDVSLLAN ENGSSLAMVE GVRSCIEYIS DFQPNCEVAI IVYDNKLRFF NLRPDLDNAQ 370 380 390 400 410 420 EYIVSELDDV FLPFYNGLFV KPGNSMKIIN DTLIKISGYI STDKYSHVPQ VCYGSALQAA 430 440 450 460 470 480 KLALDTVTGG QGGKIICSLN SLPTIGNGNL SLKRDNAHIA HVKCDNGFYK KLASDFLKSY 490 500 510 520 530 540 ISLDLYVTNA GFIDMATVGH PVEMTSGILK YYPHFQQETD AFTLVNDMVT NVSNIVGYQA 550 560 570 580 590 600 LLKVRCSTGL SVEQYYCDSS DNTDHDPIIP VLTRDTTLDV LLKYDSKIKT GTDVHFQTAL 610 620 630 640 650 660 LYTDIDGVRK VRSINTSGAV SNNIREIFKF INQNPVMRIM IKDVIKTLGD CDFVKIRRLI 670 680 690 700 710 720 DDKMVEILTQ YRGLVSSNSS TQLILPDSIK TLPAYMLAFE KSELMKPNAQ STRGNERIYD 730 740 750 760 770 780 LLKYDSLNSA QLCYKLYPQI VPFHVLLEET DLTFYDANDK LLQINSSSIN NLSVRASHSN 790 800 810 820 830 840 FINGGCYLIF QGDTIYLWFN ENTNRMLLQD LLSVDESLPV SQISLFSGTL PETGTSINQK 850 860 870 880 890 900 ASNVIKNWQQ VVNKSSLPLV LLRPNVDQYY SNVMSQLLCE DKTVNRIESY DNYLVIMHKK 910 920 IQEKLQKDDF IKVSTAATHE NIHQKFVQF

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Complete nucleotide sequence of Saccharomyces cerevisiae chromosome VIII." Johnston M., Andrews S., Brinkman R., Cooper J., Ding H., Dover J., Du Z., Favello A., Fulton L., Gattung S., Geisel C., Kirsten J., Kucaba T., Hillier L.W., Jier M., Johnston L., Langston Y., Latreille P. , Louis E.J., Macri C., Mardis E., Menezes S., Mouser L., Nhan M., Rifkin L., Riles L., St Peter H., Trevaskis E., Vaughan K., Vignati D., Wilcox L., Wohldman P., Waterston R., Wilson R., Vaudin M. Science 265:2077-2082(1994) [PubMed: 8091229] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[2]	"Evidence for overlapping and distinct functions in protein transport of coat protein Sec24p family members." Peng R., De Antoni A., Gallwitz D. J. Biol. Chem. 275:11521-11528(2000) [PubMed: 10753972] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], CHARACTERIZATION.
[3]	"LST1 is a SEC24 homologue used for selective export of the plasma membrane ATPase from the endoplasmic reticulum." Roberg K.J., Crotwell M., Espenshade P., Gimeno R., Kaiser C.A. J. Cell Biol. 145:659-672(1999) [PubMed: 10330397] [Abstract] Cited for: CHARACTERIZATION.
[4]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[5]	"Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae." Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P. J. Proteome Res. 6:1190-1197(2007) [PubMed: 17330950] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-101, MASS SPECTROMETRY.
[6]	"Proteome-wide identification of in vivo targets of DNA damage checkpoint kinases." Smolka M.B., Albuquerque C.P., Chen S.H., Zhou H. Proc. Natl. Acad. Sci. U.S.A. 104:10364-10369(2007) [PubMed: 17563356] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-110, MASS SPECTROMETRY.
[7]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed: 18407956] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-15; SER-52; SER-83; SER-85; SER-94; SER-101; SER-105; SER-110 AND THR-216, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	U00060 Genomic DNA. Translation: AAB68936.1. AJ009784 Genomic DNA. Translation: CAA08831.1.
PIR	S46728.
RefSeq	NP_011966.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
DIP	DIP:2949N.
IntAct	P38810. 15 interactions.
Proteomic databases
PeptideAtlas	P38810.
Genome annotation databases
Ensembl	YHR098C. Saccharomyces cerevisiae. [Contig view]
GeneID	856498.
GenomeReviews	Gene locus YHR098C in contig U00093_GR.
KEGG	sce:YHR098C.
NMPDR	fig|4932.3.peg.3127.
Organism-specific databases
CYGD	YHR098c.
SGD	S000001140. SFB3.
Yeast-GFP	Search...
Phylogenomic databases
HOGENOM	P38810.
OMA	P38810. NENGSSL.
Gene expression databases
ArrayExpress	P38810.
GermOnline	YHR098C. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR007123. Gelsoln. IPR012990. Sec23_24_beta_S. IPR006900. Sec23_helical. IPR006896. Sec23_trunk. IPR006895. Znf_Sec23_Sec24. [Graphical view]
Pfam	PF00626. Gelsolin. 1 hit. PF08033. Sec23_BS. 1 hit. PF04815. Sec23_helical. 1 hit. PF04811. Sec23_trunk. 1 hit. PF04810. zf-Sec23_Sec24. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	982212.

Entry information

Entry name

SFB3_YEAST

Accession

Primary (citable) accession number: P38810

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1995
Last sequence update:	February 1, 1995
Last modified:	June 16, 2009
This is version 76 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)