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P38810 (SFB3_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 121. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
SED5-binding protein 3
Alternative name(s):
Lethal with SEC13 protein 1
SEC24-related protein 3
Gene names
Name:SFB3
Synonyms:LST1
Ordered Locus Names:YHR098C
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length929 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Component of the COPII coat, that covers ER-derived vesicles involved in transport from the endoplasmic reticulum to the Golgi apparatus. COPII acts in the cytoplasm to promote the transport of secretory, plasma membrane, and vacuolar proteins from the endoplasmic reticulum to the Golgi complex.

Subunit structure

COPII is composed of at least five proteins: the SEC23/24 complex, the SEC13/31 complex and SAR1. Binds to SED5. Interacts with GHR1. Ref.6

Subcellular location

Cytoplasm By similarity. Golgi apparatus membrane By similarity. Endoplasmic reticulum membrane By similarity.

Miscellaneous

Present with 12200 molecules/cell in log phase SD medium.

Sequence similarities

Belongs to the SEC23/SEC24 family. SEC24 subfamily.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 929929SED5-binding protein 3
PRO_0000205152

Regions

Region220 – 24425Zinc finger-like

Amino acid modifications

Modified residue151Phosphoserine Ref.8
Modified residue721Phosphothreonine Ref.9
Modified residue831Phosphoserine Ref.9
Modified residue851Phosphoserine Ref.9
Modified residue941Phosphoserine Ref.8
Modified residue1011Phosphoserine Ref.7 Ref.8 Ref.9
Modified residue1101Phosphoserine Ref.9
Modified residue2161Phosphothreonine Ref.8

Sequences

Sequence LengthMass (Da)Tools
P38810 [UniParc].

Last modified February 1, 1995. Version 1.
Checksum: 6A028AD6B1A2E031

FASTA929103,950
        10         20         30         40         50         60 
MSQQNILAAS VSALSLDEST VHTGGASSKK SRRPHRAYHN FSSGTVPTLG NSPYTTPQLN 

        70         80         90        100        110        120 
QQDGFQQPQA FTPKQFGGFN NGSGSVMSTP VMVSQERFGA SEASSPYGQS MLDMTAPQPT 

       130        140        150        160        170        180 
SHIVPTQRFE DQAQYLQRSF ETCRDSVPPL PTTQFYCVDQ GSCDPHLMSL SMYNIPESEH 

       190        200        210        220        230        240 
LRAATKLPLG LTIQPFSTLT PNDAEVPTIP LPMDGTPLRC RRCRAYANPK FQFTYDSSVI 

       250        260        270        280        290        300 
CNICRVKMQV PGEHFAPMGP NGQRSDLNEK SELLHGTVDF LVPSIYNAIQ EKELLPLHYV 

       310        320        330        340        350        360 
FLIDVSLLAN ENGSSLAMVE GVRSCIEYIS DFQPNCEVAI IVYDNKLRFF NLRPDLDNAQ 

       370        380        390        400        410        420 
EYIVSELDDV FLPFYNGLFV KPGNSMKIIN DTLIKISGYI STDKYSHVPQ VCYGSALQAA 

       430        440        450        460        470        480 
KLALDTVTGG QGGKIICSLN SLPTIGNGNL SLKRDNAHIA HVKCDNGFYK KLASDFLKSY 

       490        500        510        520        530        540 
ISLDLYVTNA GFIDMATVGH PVEMTSGILK YYPHFQQETD AFTLVNDMVT NVSNIVGYQA 

       550        560        570        580        590        600 
LLKVRCSTGL SVEQYYCDSS DNTDHDPIIP VLTRDTTLDV LLKYDSKIKT GTDVHFQTAL 

       610        620        630        640        650        660 
LYTDIDGVRK VRSINTSGAV SNNIREIFKF INQNPVMRIM IKDVIKTLGD CDFVKIRRLI 

       670        680        690        700        710        720 
DDKMVEILTQ YRGLVSSNSS TQLILPDSIK TLPAYMLAFE KSELMKPNAQ STRGNERIYD 

       730        740        750        760        770        780 
LLKYDSLNSA QLCYKLYPQI VPFHVLLEET DLTFYDANDK LLQINSSSIN NLSVRASHSN 

       790        800        810        820        830        840 
FINGGCYLIF QGDTIYLWFN ENTNRMLLQD LLSVDESLPV SQISLFSGTL PETGTSINQK 

       850        860        870        880        890        900 
ASNVIKNWQQ VVNKSSLPLV LLRPNVDQYY SNVMSQLLCE DKTVNRIESY DNYLVIMHKK 

       910        920 
IQEKLQKDDF IKVSTAATHE NIHQKFVQF 

« Hide

References

« Hide 'large scale' references
[1]"Complete nucleotide sequence of Saccharomyces cerevisiae chromosome VIII."
Johnston M., Andrews S., Brinkman R., Cooper J., Ding H., Dover J., Du Z., Favello A., Fulton L., Gattung S., Geisel C., Kirsten J., Kucaba T., Hillier L.W., Jier M., Johnston L., Langston Y., Latreille P. expand/collapse author list , Louis E.J., Macri C., Mardis E., Menezes S., Mouser L., Nhan M., Rifkin L., Riles L., St Peter H., Trevaskis E., Vaughan K., Vignati D., Wilcox L., Wohldman P., Waterston R., Wilson R., Vaudin M.
Science 265:2077-2082(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[2]Saccharomyces Genome Database
Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[3]"Evidence for overlapping and distinct functions in protein transport of coat protein Sec24p family members."
Peng R., De Antoni A., Gallwitz D.
J. Biol. Chem. 275:11521-11528(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], CHARACTERIZATION.
[4]"LST1 is a SEC24 homologue used for selective export of the plasma membrane ATPase from the endoplasmic reticulum."
Roberg K.J., Crotwell M., Espenshade P., Gimeno R., Kaiser C.A.
J. Cell Biol. 145:659-672(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: CHARACTERIZATION.
[5]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[6]"The yeast orthologue of GRASP65 forms a complex with a coiled-coil protein that contributes to ER to Golgi traffic."
Behnia R., Barr F.A., Flanagan J.J., Barlowe C., Munro S.
J. Cell Biol. 176:255-261(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH GRH1.
[7]"Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-101, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Strain: ADR376.
[8]"A multidimensional chromatography technology for in-depth phosphoproteome analysis."
Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-15; SER-94; SER-101 AND THR-216, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[9]"Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-72; SER-83; SER-85; SER-101 AND SER-110, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U00060 Genomic DNA. Translation: AAB68936.1.
AJ009784 Genomic DNA. Translation: CAA08831.1.
BK006934 Genomic DNA. Translation: DAA06792.1.
PIRS46728.
RefSeqNP_011966.1. NM_001179228.1.

3D structure databases

ProteinModelPortalP38810.
SMRP38810. Positions 121-906.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid36531. 22 interactions.
DIPDIP-2949N.
IntActP38810. 10 interactions.
MINTMINT-509354.
STRING4932.YHR098C.

Proteomic databases

PaxDbP38810.
PeptideAtlasP38810.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYHR098C; YHR098C; YHR098C.
GeneID856498.
KEGGsce:YHR098C.

Organism-specific databases

CYGDYHR098c.
SGDS000001140. SFB3.

Phylogenomic databases

eggNOGCOG5028.
HOGENOMHOG000141722.
OMANTDKERT.
OrthoDBEOG71K6BX.

Enzyme and pathway databases

BioCycYEAST:G3O-31143-MONOMER.

Gene expression databases

GenevestigatorP38810.

Family and domain databases

Gene3D3.40.50.410. 1 hit.
InterProIPR007123. Gelsolin-like_dom.
IPR006900. Sec23/24_helical_dom.
IPR006896. Sec23/24_trunk_dom.
IPR012990. Sec23_24_beta_S.
IPR002035. VWF_A.
IPR006895. Znf_Sec23_Sec24.
[Graphical view]
PfamPF00626. Gelsolin. 1 hit.
PF08033. Sec23_BS. 1 hit.
PF04815. Sec23_helical. 1 hit.
PF04811. Sec23_trunk. 1 hit.
PF04810. zf-Sec23_Sec24. 1 hit.
[Graphical view]
SUPFAMSSF53300. SSF53300. 1 hit.
SSF81811. SSF81811. 1 hit.
SSF82919. SSF82919. 1 hit.
ProtoNetSearch...

Other

NextBio982212.
PROP38810.

Entry information

Entry nameSFB3_YEAST
AccessionPrimary (citable) accession number: P38810
Secondary accession number(s): D3DL48
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: February 1, 1995
Last modified: April 16, 2014
This is version 121 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome VIII

Yeast (Saccharomyces cerevisiae) chromosome VIII: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families