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P38810

- SFB3_YEAST

UniProt

P38810 - SFB3_YEAST

Protein

SED5-binding protein 3

Gene

SFB3

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 124 (01 Oct 2014)
      Sequence version 1 (01 Feb 1995)
      Previous versions | rss
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    Functioni

    Component of the COPII coat, that covers ER-derived vesicles involved in transport from the endoplasmic reticulum to the Golgi apparatus. COPII acts in the cytoplasm to promote the transport of secretory, plasma membrane, and vacuolar proteins from the endoplasmic reticulum to the Golgi complex.

    GO - Molecular functioni

    1. signal sequence binding Source: SGD
    2. zinc ion binding Source: InterPro

    GO - Biological processi

    1. cargo loading into COPII-coated vesicle Source: SGD
    2. intracellular protein transport Source: InterPro

    Keywords - Biological processi

    ER-Golgi transport, Protein transport, Transport

    Enzyme and pathway databases

    BioCyciYEAST:G3O-31143-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    SED5-binding protein 3
    Alternative name(s):
    Lethal with SEC13 protein 1
    SEC24-related protein 3
    Gene namesi
    Name:SFB3
    Synonyms:LST1
    Ordered Locus Names:YHR098C
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome VIII

    Organism-specific databases

    CYGDiYHR098c.
    SGDiS000001140. SFB3.

    Subcellular locationi

    Cytoplasm By similarity. Golgi apparatus membrane By similarity. Endoplasmic reticulum membrane By similarity

    GO - Cellular componenti

    1. COPII vesicle coat Source: SGD
    2. endoplasmic reticulum membrane Source: UniProtKB-SubCell
    3. Golgi membrane Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm, Endoplasmic reticulum, Golgi apparatus, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 929929SED5-binding protein 3PRO_0000205152Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei15 – 151Phosphoserine1 Publication
    Modified residuei72 – 721Phosphothreonine1 Publication
    Modified residuei83 – 831Phosphoserine1 Publication
    Modified residuei85 – 851Phosphoserine1 Publication
    Modified residuei94 – 941Phosphoserine1 Publication
    Modified residuei101 – 1011Phosphoserine3 Publications
    Modified residuei110 – 1101Phosphoserine1 Publication
    Modified residuei216 – 2161Phosphothreonine1 Publication

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    MaxQBiP38810.
    PaxDbiP38810.
    PeptideAtlasiP38810.

    Expressioni

    Gene expression databases

    GenevestigatoriP38810.

    Interactioni

    Subunit structurei

    COPII is composed of at least five proteins: the SEC23/24 complex, the SEC13/31 complex and SAR1. Binds to SED5. Interacts with GHR1.1 Publication

    Protein-protein interaction databases

    BioGridi36531. 22 interactions.
    DIPiDIP-2949N.
    IntActiP38810. 10 interactions.
    MINTiMINT-509354.
    STRINGi4932.YHR098C.

    Structurei

    3D structure databases

    ProteinModelPortaliP38810.
    SMRiP38810. Positions 125-905.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni220 – 24425Zinc finger-likeAdd
    BLAST

    Sequence similaritiesi

    Belongs to the SEC23/SEC24 family. SEC24 subfamily.Curated

    Phylogenomic databases

    eggNOGiCOG5028.
    HOGENOMiHOG000141722.
    OMAiATHENIH.
    OrthoDBiEOG71K6BX.

    Family and domain databases

    Gene3Di3.40.20.10. 1 hit.
    3.40.50.410. 1 hit.
    InterProiIPR029006. ADF-H/Gelsolin-like_dom.
    IPR007123. Gelsolin-like_dom.
    IPR006900. Sec23/24_helical_dom.
    IPR006896. Sec23/24_trunk_dom.
    IPR012990. Sec23_24_beta_S.
    IPR002035. VWF_A.
    IPR006895. Znf_Sec23_Sec24.
    [Graphical view]
    PfamiPF00626. Gelsolin. 1 hit.
    PF08033. Sec23_BS. 1 hit.
    PF04815. Sec23_helical. 1 hit.
    PF04811. Sec23_trunk. 1 hit.
    PF04810. zf-Sec23_Sec24. 1 hit.
    [Graphical view]
    SUPFAMiSSF53300. SSF53300. 1 hit.
    SSF81811. SSF81811. 1 hit.
    SSF82919. SSF82919. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    P38810-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSQQNILAAS VSALSLDEST VHTGGASSKK SRRPHRAYHN FSSGTVPTLG    50
    NSPYTTPQLN QQDGFQQPQA FTPKQFGGFN NGSGSVMSTP VMVSQERFGA 100
    SEASSPYGQS MLDMTAPQPT SHIVPTQRFE DQAQYLQRSF ETCRDSVPPL 150
    PTTQFYCVDQ GSCDPHLMSL SMYNIPESEH LRAATKLPLG LTIQPFSTLT 200
    PNDAEVPTIP LPMDGTPLRC RRCRAYANPK FQFTYDSSVI CNICRVKMQV 250
    PGEHFAPMGP NGQRSDLNEK SELLHGTVDF LVPSIYNAIQ EKELLPLHYV 300
    FLIDVSLLAN ENGSSLAMVE GVRSCIEYIS DFQPNCEVAI IVYDNKLRFF 350
    NLRPDLDNAQ EYIVSELDDV FLPFYNGLFV KPGNSMKIIN DTLIKISGYI 400
    STDKYSHVPQ VCYGSALQAA KLALDTVTGG QGGKIICSLN SLPTIGNGNL 450
    SLKRDNAHIA HVKCDNGFYK KLASDFLKSY ISLDLYVTNA GFIDMATVGH 500
    PVEMTSGILK YYPHFQQETD AFTLVNDMVT NVSNIVGYQA LLKVRCSTGL 550
    SVEQYYCDSS DNTDHDPIIP VLTRDTTLDV LLKYDSKIKT GTDVHFQTAL 600
    LYTDIDGVRK VRSINTSGAV SNNIREIFKF INQNPVMRIM IKDVIKTLGD 650
    CDFVKIRRLI DDKMVEILTQ YRGLVSSNSS TQLILPDSIK TLPAYMLAFE 700
    KSELMKPNAQ STRGNERIYD LLKYDSLNSA QLCYKLYPQI VPFHVLLEET 750
    DLTFYDANDK LLQINSSSIN NLSVRASHSN FINGGCYLIF QGDTIYLWFN 800
    ENTNRMLLQD LLSVDESLPV SQISLFSGTL PETGTSINQK ASNVIKNWQQ 850
    VVNKSSLPLV LLRPNVDQYY SNVMSQLLCE DKTVNRIESY DNYLVIMHKK 900
    IQEKLQKDDF IKVSTAATHE NIHQKFVQF 929
    Length:929
    Mass (Da):103,950
    Last modified:February 1, 1995 - v1
    Checksum:i6A028AD6B1A2E031
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U00060 Genomic DNA. Translation: AAB68936.1.
    AJ009784 Genomic DNA. Translation: CAA08831.1.
    BK006934 Genomic DNA. Translation: DAA06792.1.
    PIRiS46728.
    RefSeqiNP_011966.1. NM_001179228.1.

    Genome annotation databases

    EnsemblFungiiYHR098C; YHR098C; YHR098C.
    GeneIDi856498.
    KEGGisce:YHR098C.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U00060 Genomic DNA. Translation: AAB68936.1 .
    AJ009784 Genomic DNA. Translation: CAA08831.1 .
    BK006934 Genomic DNA. Translation: DAA06792.1 .
    PIRi S46728.
    RefSeqi NP_011966.1. NM_001179228.1.

    3D structure databases

    ProteinModelPortali P38810.
    SMRi P38810. Positions 125-905.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 36531. 22 interactions.
    DIPi DIP-2949N.
    IntActi P38810. 10 interactions.
    MINTi MINT-509354.
    STRINGi 4932.YHR098C.

    Proteomic databases

    MaxQBi P38810.
    PaxDbi P38810.
    PeptideAtlasi P38810.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YHR098C ; YHR098C ; YHR098C .
    GeneIDi 856498.
    KEGGi sce:YHR098C.

    Organism-specific databases

    CYGDi YHR098c.
    SGDi S000001140. SFB3.

    Phylogenomic databases

    eggNOGi COG5028.
    HOGENOMi HOG000141722.
    OMAi ATHENIH.
    OrthoDBi EOG71K6BX.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-31143-MONOMER.

    Miscellaneous databases

    NextBioi 982212.
    PROi P38810.

    Gene expression databases

    Genevestigatori P38810.

    Family and domain databases

    Gene3Di 3.40.20.10. 1 hit.
    3.40.50.410. 1 hit.
    InterProi IPR029006. ADF-H/Gelsolin-like_dom.
    IPR007123. Gelsolin-like_dom.
    IPR006900. Sec23/24_helical_dom.
    IPR006896. Sec23/24_trunk_dom.
    IPR012990. Sec23_24_beta_S.
    IPR002035. VWF_A.
    IPR006895. Znf_Sec23_Sec24.
    [Graphical view ]
    Pfami PF00626. Gelsolin. 1 hit.
    PF08033. Sec23_BS. 1 hit.
    PF04815. Sec23_helical. 1 hit.
    PF04811. Sec23_trunk. 1 hit.
    PF04810. zf-Sec23_Sec24. 1 hit.
    [Graphical view ]
    SUPFAMi SSF53300. SSF53300. 1 hit.
    SSF81811. SSF81811. 1 hit.
    SSF82919. SSF82919. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    2. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    3. "Evidence for overlapping and distinct functions in protein transport of coat protein Sec24p family members."
      Peng R., De Antoni A., Gallwitz D.
      J. Biol. Chem. 275:11521-11528(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], CHARACTERIZATION.
    4. "LST1 is a SEC24 homologue used for selective export of the plasma membrane ATPase from the endoplasmic reticulum."
      Roberg K.J., Crotwell M., Espenshade P., Gimeno R., Kaiser C.A.
      J. Cell Biol. 145:659-672(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: CHARACTERIZATION.
    5. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
    6. "The yeast orthologue of GRASP65 forms a complex with a coiled-coil protein that contributes to ER to Golgi traffic."
      Behnia R., Barr F.A., Flanagan J.J., Barlowe C., Munro S.
      J. Cell Biol. 176:255-261(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH GRH1.
    7. "Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
      Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
      J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-101, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Strain: ADR376.
    8. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
      Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
      Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-15; SER-94; SER-101 AND THR-216, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    9. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
      Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
      Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-72; SER-83; SER-85; SER-101 AND SER-110, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiSFB3_YEAST
    AccessioniPrimary (citable) accession number: P38810
    Secondary accession number(s): D3DL48
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 1, 1995
    Last sequence update: February 1, 1995
    Last modified: October 1, 2014
    This is version 124 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 12200 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families
    2. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    3. Yeast chromosome VIII
      Yeast (Saccharomyces cerevisiae) chromosome VIII: entries and gene names

    External Data

    Dasty 3