SED5-binding protein 3 - Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

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P38810 (SFB3_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified December 14, 2011. Version 100. History...

Clusters with 100%, 90%, 50% identity |

Documents (3) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
SED5-binding protein 3

Alternative name(s):
Lethal with SEC13 protein 1
SEC24-related protein 3

Gene names

Name:	SFB3
Synonyms:	LST1
Ordered Locus Names:	YHR098C

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	929 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Component of the COPII coat, that covers ER-derived vesicles involved in transport from the endoplasmic reticulum to the Golgi apparatus. COPII acts in the cytoplasm to promote the transport of secretory, plasma membrane, and vacuolar proteins from the endoplasmic reticulum to the Golgi complex.
Subunit structure	COPII is composed of at least five proteins: the SEC23/24 complex, the SEC13/31 complex and SAR1. Binds to SED5.
Subcellular location	Cytoplasm By similarity. Golgi apparatus membrane By similarity. Endoplasmic reticulum membrane By similarity.
Miscellaneous	Present with 12200 molecules/cell in log phase SD medium. Ref.5
Sequence similarities	Belongs to the SEC23/SEC24 family. SEC24 subfamily.

Ontologies

Keywords
Biological process	ER-Golgi transport Protein transport Transport
Cellular component	Cytoplasm Endoplasmic reticulum Golgi apparatus Membrane
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	cargo loading into COPII-coated vesicle Inferred from direct assay. Source: SGD intracellular protein transport Inferred from electronic annotation. Source: InterPro
Cellular component	COPII vesicle coat Inferred from direct assay. Source: SGD Golgi membrane Inferred from electronic annotation. Source: UniProtKB-SubCell endoplasmic reticulum membrane Inferred from electronic annotation. Source: UniProtKB-SubCell mating projection tip Inferred from direct assay. Source: SGD
Molecular function	zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 929

929

SED5-binding protein 3

PRO_0000205152

Regions

Region

220 – 244

Zinc finger-like

Amino acid modifications

Modified residue

Phosphoserine Ref.8

Modified residue

Phosphoserine Ref.8

Modified residue

Phosphoserine Ref.8

Modified residue

Phosphoserine Ref.8

Modified residue

Phosphoserine Ref.8

Modified residue

101

Phosphoserine Ref.6 Ref.8

Modified residue

105

Phosphoserine Ref.8

Modified residue

110

Phosphoserine Ref.7 Ref.8

Modified residue

216

Phosphothreonine Ref.8

Sequences

Sequence

Length

Mass (Da)

Tools

P38810 [UniParc].

Last modified February 1, 1995. Version 1.
Checksum: 6A028AD6B1A2E031
Show »

FASTA

929

103,950

        10         20         30         40         50         60 
MSQQNILAAS VSALSLDEST VHTGGASSKK SRRPHRAYHN FSSGTVPTLG NSPYTTPQLN 

        70         80         90        100        110        120 
QQDGFQQPQA FTPKQFGGFN NGSGSVMSTP VMVSQERFGA SEASSPYGQS MLDMTAPQPT 

       130        140        150        160        170        180 
SHIVPTQRFE DQAQYLQRSF ETCRDSVPPL PTTQFYCVDQ GSCDPHLMSL SMYNIPESEH 

       190        200        210        220        230        240 
LRAATKLPLG LTIQPFSTLT PNDAEVPTIP LPMDGTPLRC RRCRAYANPK FQFTYDSSVI 

       250        260        270        280        290        300 
CNICRVKMQV PGEHFAPMGP NGQRSDLNEK SELLHGTVDF LVPSIYNAIQ EKELLPLHYV 

       310        320        330        340        350        360 
FLIDVSLLAN ENGSSLAMVE GVRSCIEYIS DFQPNCEVAI IVYDNKLRFF NLRPDLDNAQ 

       370        380        390        400        410        420 
EYIVSELDDV FLPFYNGLFV KPGNSMKIIN DTLIKISGYI STDKYSHVPQ VCYGSALQAA 

       430        440        450        460        470        480 
KLALDTVTGG QGGKIICSLN SLPTIGNGNL SLKRDNAHIA HVKCDNGFYK KLASDFLKSY 

       490        500        510        520        530        540 
ISLDLYVTNA GFIDMATVGH PVEMTSGILK YYPHFQQETD AFTLVNDMVT NVSNIVGYQA 

       550        560        570        580        590        600 
LLKVRCSTGL SVEQYYCDSS DNTDHDPIIP VLTRDTTLDV LLKYDSKIKT GTDVHFQTAL 

       610        620        630        640        650        660 
LYTDIDGVRK VRSINTSGAV SNNIREIFKF INQNPVMRIM IKDVIKTLGD CDFVKIRRLI 

       670        680        690        700        710        720 
DDKMVEILTQ YRGLVSSNSS TQLILPDSIK TLPAYMLAFE KSELMKPNAQ STRGNERIYD 

       730        740        750        760        770        780 
LLKYDSLNSA QLCYKLYPQI VPFHVLLEET DLTFYDANDK LLQINSSSIN NLSVRASHSN 

       790        800        810        820        830        840 
FINGGCYLIF QGDTIYLWFN ENTNRMLLQD LLSVDESLPV SQISLFSGTL PETGTSINQK 

       850        860        870        880        890        900 
ASNVIKNWQQ VVNKSSLPLV LLRPNVDQYY SNVMSQLLCE DKTVNRIESY DNYLVIMHKK 

       910        920 
IQEKLQKDDF IKVSTAATHE NIHQKFVQF

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Complete nucleotide sequence of Saccharomyces cerevisiae chromosome VIII." Johnston M., Andrews S., Brinkman R., Cooper J., Ding H., Dover J., Du Z., Favello A., Fulton L., Gattung S., Geisel C., Kirsten J., Kucaba T., Hillier L.W., Jier M., Johnston L., Langston Y., Latreille P. Vaudin M. Science 265:2077-2082(1994) [PubMed: 8091229] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[2]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[3]	"Evidence for overlapping and distinct functions in protein transport of coat protein Sec24p family members." Peng R., De Antoni A., Gallwitz D. J. Biol. Chem. 275:11521-11528(2000) [PubMed: 10753972] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], CHARACTERIZATION.
[4]	"LST1 is a SEC24 homologue used for selective export of the plasma membrane ATPase from the endoplasmic reticulum." Roberg K.J., Crotwell M., Espenshade P., Gimeno R., Kaiser C.A. J. Cell Biol. 145:659-672(1999) [PubMed: 10330397] [Abstract] Cited for: CHARACTERIZATION.
[5]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[6]	"Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae." Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P. J. Proteome Res. 6:1190-1197(2007) [PubMed: 17330950] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-101, MASS SPECTROMETRY. Strain: ADR376.
[7]	"Proteome-wide identification of in vivo targets of DNA damage checkpoint kinases." Smolka M.B., Albuquerque C.P., Chen S.H., Zhou H. Proc. Natl. Acad. Sci. U.S.A. 104:10364-10369(2007) [PubMed: 17563356] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-110, MASS SPECTROMETRY.
[8]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed: 18407956] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-15; SER-52; SER-83; SER-85; SER-94; SER-101; SER-105; SER-110 AND THR-216, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U00060 Genomic DNA. Translation: AAB68936.1. AJ009784 Genomic DNA. Translation: CAA08831.1. BK006934 Genomic DNA. Translation: DAA06792.1.
PIR	S46728.
RefSeq	NP_011966.1. NM_001179228.1.
3D structure databases
ProteinModelPortal	P38810.
SMR	P38810. Positions 125-906.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-2949N.
IntAct	P38810. 13 interactions.
MINT	MINT-509354.
STRING	P38810.
Proteomic databases
PeptideAtlas	P38810.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YHR098C; YHR098C; YHR098C.
GeneID	856498.
KEGG	sce:YHR098C.
NMPDR	fig\|4932.3.peg.3127.
Organism-specific databases
CYGD	YHR098c.
SGD	S000001140. SFB3.
Phylogenomic databases
eggNOG	fuNOG07602.
GeneTree	EFGT00050000002844.
HOGENOM	HBG203772.
OMA	GGCYLIF.
OrthoDB	EOG49S9FH.
Gene expression databases
ArrayExpress	P38810.
Genevestigator	P38810.
GermOnline	YHR098C. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR007123. Gelsolin_dom. IPR006900. Sec23/24_helical_dom. IPR006896. Sec23/24_trunk_dom. IPR012990. Sec23_24_beta_S. IPR006895. Znf_Sec23_Sec24. [Graphical view]
Pfam	PF00626. Gelsolin. 1 hit. PF08033. Sec23_BS. 1 hit. PF04815. Sec23_helical. 1 hit. PF04811. Sec23_trunk. 1 hit. PF04810. zf-Sec23_Sec24. 1 hit. [Graphical view]
SUPFAM	SSF81811. Sec23_helical. 1 hit. SSF82919. Znf_Sec23_Sec24. 1 hit.
ProtoNet	Search...
Other
NextBio	982212.

Entry information

Entry name

SFB3_YEAST

Accession

Primary (citable) accession number: P38810
Secondary accession number(s): D3DL48

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1995
Last sequence update:	February 1, 1995
Last modified:	December 14, 2011
This is version 100 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program

Relevant documents

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

Yeast chromosome VIII

Yeast (Saccharomyces cerevisiae) chromosome VIII: entries and gene names

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Amino acid modifications

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents