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Protein

SED5-binding protein 3

Gene

SFB3

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Component of the COPII coat, that covers ER-derived vesicles involved in transport from the endoplasmic reticulum to the Golgi apparatus. COPII acts in the cytoplasm to promote the transport of secretory, plasma membrane, and vacuolar proteins from the endoplasmic reticulum to the Golgi complex.

GO - Molecular functioni

  1. signal sequence binding Source: SGD
  2. zinc ion binding Source: InterPro

GO - Biological processi

  1. cargo loading into COPII-coated vesicle Source: SGD
  2. intracellular protein transport Source: InterPro
Complete GO annotation...

Keywords - Biological processi

ER-Golgi transport, Protein transport, Transport

Enzyme and pathway databases

BioCyciYEAST:G3O-31143-MONOMER.
ReactomeiREACT_298514. COPII (Coat Protein 2) Mediated Vesicle Transport.

Names & Taxonomyi

Protein namesi
Recommended name:
SED5-binding protein 3
Alternative name(s):
Lethal with SEC13 protein 1
SEC24-related protein 3
Gene namesi
Name:SFB3
Synonyms:LST1
Ordered Locus Names:YHR098C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311 Componenti: Chromosome VIII

Organism-specific databases

CYGDiYHR098c.
SGDiS000001140. SFB3.

Subcellular locationi

Cytoplasm By similarity. Golgi apparatus membrane By similarity. Endoplasmic reticulum membrane By similarity

GO - Cellular componenti

  1. COPII vesicle coat Source: SGD
  2. endoplasmic reticulum membrane Source: UniProtKB-SubCell
  3. Golgi membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Endoplasmic reticulum, Golgi apparatus, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 929929SED5-binding protein 3PRO_0000205152Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei15 – 151Phosphoserine1 Publication
Modified residuei72 – 721Phosphothreonine1 Publication
Modified residuei83 – 831Phosphoserine1 Publication
Modified residuei85 – 851Phosphoserine1 Publication
Modified residuei94 – 941Phosphoserine1 Publication
Modified residuei101 – 1011Phosphoserine3 Publications
Modified residuei110 – 1101Phosphoserine1 Publication
Modified residuei216 – 2161Phosphothreonine1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP38810.
PaxDbiP38810.
PeptideAtlasiP38810.

Expressioni

Gene expression databases

GenevestigatoriP38810.

Interactioni

Subunit structurei

COPII is composed of at least five proteins: the SEC23/24 complex, the SEC13/31 complex and SAR1. Binds to SED5. Interacts with GHR1.1 Publication

Protein-protein interaction databases

BioGridi36531. 23 interactions.
DIPiDIP-2949N.
IntActiP38810. 10 interactions.
MINTiMINT-509354.
STRINGi4932.YHR098C.

Structurei

3D structure databases

ProteinModelPortaliP38810.
SMRiP38810. Positions 125-905.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni220 – 24425Zinc finger-likeAdd
BLAST

Sequence similaritiesi

Belongs to the SEC23/SEC24 family. SEC24 subfamily.Curated

Phylogenomic databases

eggNOGiCOG5028.
HOGENOMiHOG000141722.
InParanoidiP38810.
OMAiYLIFQGD.
OrthoDBiEOG71K6BX.

Family and domain databases

Gene3Di3.40.20.10. 1 hit.
3.40.50.410. 1 hit.
InterProiIPR029006. ADF-H/Gelsolin-like_dom.
IPR007123. Gelsolin-like_dom.
IPR006900. Sec23/24_helical_dom.
IPR006896. Sec23/24_trunk_dom.
IPR012990. Sec23_24_beta_S.
IPR002035. VWF_A.
IPR006895. Znf_Sec23_Sec24.
[Graphical view]
PfamiPF00626. Gelsolin. 1 hit.
PF08033. Sec23_BS. 1 hit.
PF04815. Sec23_helical. 1 hit.
PF04811. Sec23_trunk. 1 hit.
PF04810. zf-Sec23_Sec24. 1 hit.
[Graphical view]
SUPFAMiSSF53300. SSF53300. 1 hit.
SSF81811. SSF81811. 1 hit.
SSF82919. SSF82919. 1 hit.

Sequencei

Sequence statusi: Complete.

P38810-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSQQNILAAS VSALSLDEST VHTGGASSKK SRRPHRAYHN FSSGTVPTLG
60 70 80 90 100
NSPYTTPQLN QQDGFQQPQA FTPKQFGGFN NGSGSVMSTP VMVSQERFGA
110 120 130 140 150
SEASSPYGQS MLDMTAPQPT SHIVPTQRFE DQAQYLQRSF ETCRDSVPPL
160 170 180 190 200
PTTQFYCVDQ GSCDPHLMSL SMYNIPESEH LRAATKLPLG LTIQPFSTLT
210 220 230 240 250
PNDAEVPTIP LPMDGTPLRC RRCRAYANPK FQFTYDSSVI CNICRVKMQV
260 270 280 290 300
PGEHFAPMGP NGQRSDLNEK SELLHGTVDF LVPSIYNAIQ EKELLPLHYV
310 320 330 340 350
FLIDVSLLAN ENGSSLAMVE GVRSCIEYIS DFQPNCEVAI IVYDNKLRFF
360 370 380 390 400
NLRPDLDNAQ EYIVSELDDV FLPFYNGLFV KPGNSMKIIN DTLIKISGYI
410 420 430 440 450
STDKYSHVPQ VCYGSALQAA KLALDTVTGG QGGKIICSLN SLPTIGNGNL
460 470 480 490 500
SLKRDNAHIA HVKCDNGFYK KLASDFLKSY ISLDLYVTNA GFIDMATVGH
510 520 530 540 550
PVEMTSGILK YYPHFQQETD AFTLVNDMVT NVSNIVGYQA LLKVRCSTGL
560 570 580 590 600
SVEQYYCDSS DNTDHDPIIP VLTRDTTLDV LLKYDSKIKT GTDVHFQTAL
610 620 630 640 650
LYTDIDGVRK VRSINTSGAV SNNIREIFKF INQNPVMRIM IKDVIKTLGD
660 670 680 690 700
CDFVKIRRLI DDKMVEILTQ YRGLVSSNSS TQLILPDSIK TLPAYMLAFE
710 720 730 740 750
KSELMKPNAQ STRGNERIYD LLKYDSLNSA QLCYKLYPQI VPFHVLLEET
760 770 780 790 800
DLTFYDANDK LLQINSSSIN NLSVRASHSN FINGGCYLIF QGDTIYLWFN
810 820 830 840 850
ENTNRMLLQD LLSVDESLPV SQISLFSGTL PETGTSINQK ASNVIKNWQQ
860 870 880 890 900
VVNKSSLPLV LLRPNVDQYY SNVMSQLLCE DKTVNRIESY DNYLVIMHKK
910 920
IQEKLQKDDF IKVSTAATHE NIHQKFVQF
Length:929
Mass (Da):103,950
Last modified:January 31, 1995 - v1
Checksum:i6A028AD6B1A2E031
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00060 Genomic DNA. Translation: AAB68936.1.
AJ009784 Genomic DNA. Translation: CAA08831.1.
BK006934 Genomic DNA. Translation: DAA06792.1.
PIRiS46728.
RefSeqiNP_011966.1. NM_001179228.1.

Genome annotation databases

EnsemblFungiiYHR098C; YHR098C; YHR098C.
GeneIDi856498.
KEGGisce:YHR098C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00060 Genomic DNA. Translation: AAB68936.1.
AJ009784 Genomic DNA. Translation: CAA08831.1.
BK006934 Genomic DNA. Translation: DAA06792.1.
PIRiS46728.
RefSeqiNP_011966.1. NM_001179228.1.

3D structure databases

ProteinModelPortaliP38810.
SMRiP38810. Positions 125-905.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi36531. 23 interactions.
DIPiDIP-2949N.
IntActiP38810. 10 interactions.
MINTiMINT-509354.
STRINGi4932.YHR098C.

Proteomic databases

MaxQBiP38810.
PaxDbiP38810.
PeptideAtlasiP38810.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYHR098C; YHR098C; YHR098C.
GeneIDi856498.
KEGGisce:YHR098C.

Organism-specific databases

CYGDiYHR098c.
SGDiS000001140. SFB3.

Phylogenomic databases

eggNOGiCOG5028.
HOGENOMiHOG000141722.
InParanoidiP38810.
OMAiYLIFQGD.
OrthoDBiEOG71K6BX.

Enzyme and pathway databases

BioCyciYEAST:G3O-31143-MONOMER.
ReactomeiREACT_298514. COPII (Coat Protein 2) Mediated Vesicle Transport.

Miscellaneous databases

NextBioi982212.
PROiP38810.

Gene expression databases

GenevestigatoriP38810.

Family and domain databases

Gene3Di3.40.20.10. 1 hit.
3.40.50.410. 1 hit.
InterProiIPR029006. ADF-H/Gelsolin-like_dom.
IPR007123. Gelsolin-like_dom.
IPR006900. Sec23/24_helical_dom.
IPR006896. Sec23/24_trunk_dom.
IPR012990. Sec23_24_beta_S.
IPR002035. VWF_A.
IPR006895. Znf_Sec23_Sec24.
[Graphical view]
PfamiPF00626. Gelsolin. 1 hit.
PF08033. Sec23_BS. 1 hit.
PF04815. Sec23_helical. 1 hit.
PF04811. Sec23_trunk. 1 hit.
PF04810. zf-Sec23_Sec24. 1 hit.
[Graphical view]
SUPFAMiSSF53300. SSF53300. 1 hit.
SSF81811. SSF81811. 1 hit.
SSF82919. SSF82919. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  2. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  3. "Evidence for overlapping and distinct functions in protein transport of coat protein Sec24p family members."
    Peng R., De Antoni A., Gallwitz D.
    J. Biol. Chem. 275:11521-11528(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], CHARACTERIZATION.
  4. "LST1 is a SEC24 homologue used for selective export of the plasma membrane ATPase from the endoplasmic reticulum."
    Roberg K.J., Crotwell M., Espenshade P., Gimeno R., Kaiser C.A.
    J. Cell Biol. 145:659-672(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: CHARACTERIZATION.
  5. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  6. "The yeast orthologue of GRASP65 forms a complex with a coiled-coil protein that contributes to ER to Golgi traffic."
    Behnia R., Barr F.A., Flanagan J.J., Barlowe C., Munro S.
    J. Cell Biol. 176:255-261(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH GRH1.
  7. "Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
    Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
    J. Proteome Res. 6:1190-1197(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-101, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Strain: ADR376.
  8. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
    Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
    Mol. Cell. Proteomics 7:1389-1396(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-15; SER-94; SER-101 AND THR-216, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  9. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
    Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
    Science 325:1682-1686(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-72; SER-83; SER-85; SER-101 AND SER-110, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiSFB3_YEAST
AccessioniPrimary (citable) accession number: P38810
Secondary accession number(s): D3DL48
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 31, 1995
Last sequence update: January 31, 1995
Last modified: March 31, 2015
This is version 128 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 12200 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome VIII
    Yeast (Saccharomyces cerevisiae) chromosome VIII: entries and gene names

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.