Ribosome production factor 1 - Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

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P38805 (RPF1_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified April 3, 2013. Version 94. History...

Clusters with 100%, 90%, 50% identity |

Documents (3) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Ribosome production factor 1

Alternative name(s):
Ribosome biogenesis protein RPF1

Gene names

Name:	RPF1
Ordered Locus Names:	YHR088W

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces ›

Protein attributes

Sequence length	295 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Essential protein. Required for biogenesis of the 60S ribosomal subunit. Ref.3 Ref.4
Subunit structure	Part of a complex that includes BRX1, RPF1, RPF2 and SSF1 or SSF2.
Subcellular location	Nucleus › nucleolus Ref.3 Ref.4.
Miscellaneous	Present with 784 molecules/cell in log phase SD medium.
Sequence similarities	Contains 1 Brix domain.

Ontologies

Keywords
Biological process	Ribosome biogenesis rRNA processing
Cellular component	Nucleus
Ligand	RNA-binding rRNA-binding
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	maturation of 5.8S rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) Inferred from mutant phenotype Ref.3. Source: SGD maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) Inferred from mutant phenotype Ref.3. Source: SGD ribosomal large subunit assembly Inferred from physical interaction PubMed 11864607. Source: SGD ribosomal large subunit export from nucleus Inferred from mutant phenotype PubMed 16581791. Source: SGD
Cellular_component	nucleolus Inferred from direct assay Ref.3. Source: SGD preribosome, large subunit precursor Inferred from direct assay PubMed 11583614 PubMed 17443350. Source: SGD
Molecular_function	rRNA primary transcript binding Inferred from physical interaction Ref.3. Source: SGD
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 295

295

Ribosome production factor 1

PRO_0000120255

Regions

Domain

93 – 276

184

Brix

Region

254 – 271

RNA-binding

Sequences

Sequence

Length

Mass (Da)

Tools

P38805 [UniParc].

Last modified February 1, 1995. Version 1.
Checksum: EC6EDB2FCE252D4A
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FASTA

295

35,121

        10         20         30         40         50         60 
MALGNEINIT NKLKRQEIFA DIKHEKNKER HTMRRKRAKE ERENPELREQ RLKENVTQTI 

        70         80         90        100        110        120 
ENTRVYDETI NKEVEGDEDD LMRYFNSNSN EPPKIFLTTN VNAKKSAYEF ANILIEILPN 

       130        140        150        160        170        180 
VTFVKRKFGY KLKEISDICI KRNFTDIVII NEDKKKVTGL TFIHLPEGPT FYFKLSSFVE 

       190        200        210        220        230        240 
VKKIVGHGRP TSHIPELILN NFQTRLGQTV GRLFQSILPQ NPDIEGRQVI TLHNQRDYIF 

       250        260        270        280        290 
FRRHRYVFKD NERVGLQELG PQFTLKLKRL QRGIKEETEW EHKPEMDKEK KKFYL

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Complete nucleotide sequence of Saccharomyces cerevisiae chromosome VIII." Johnston M., Andrews S., Brinkman R., Cooper J., Ding H., Dover J., Du Z., Favello A., Fulton L., Gattung S., Geisel C., Kirsten J., Kucaba T., Hillier L.W., Jier M., Johnston L., Langston Y., Latreille P. Vaudin M. Science 265:2077-2082(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[2]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[3]	"The sigma(70)-like motif: a eukaryotic RNA binding domain unique to a superfamily of proteins required for ribosome biogenesis." Wehner K.A., Baserga S.J. Mol. Cell 9:329-339(2002) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION.
[4]	"Functional analysis in yeast of the Brix protein superfamily involved in the biogenesis of ribosomes." Bogengruber E., Briza P., Doppler E., Wimmer H., Koller L., Fasiolo F., Senger B., Hegemann J.H., Breitenbach M. FEMS Yeast Res. 3:35-43(2003) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION, POSSIBLE COMPLEX COMPOSITION.
[5]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U00060 Genomic DNA. Translation: AAB68926.1. BK006934 Genomic DNA. Translation: DAA06784.1.
PIR	S46718.
RefSeq	NP_011956.1. NM_001179218.1.
3D structure databases
ProteinModelPortal	P38805.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-6511N.
IntAct	P38805. 25 interactions.
MINT	MINT-634203.
STRING	4932.YHR088W.
Proteomic databases
PaxDb	P38805.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YHR088W; YHR088W; YHR088W.
GeneID	856488.
KEGG	sce:YHR088W.
Organism-specific databases
CYGD	YHR088w.
SGD	S000001130. RPF1.
Phylogenomic databases
eggNOG	COG2136.
GeneTree	ENSGT00550000074691.
HOGENOM	HOG000172062.
KO	K14846.
OMA	IENQRVY.
OrthoDB	EOG4JT3FB.
Gene expression databases
Genevestigator	P38805.
GermOnline	YHR088W. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR004154. Anticodon-bd. IPR007109. Brix. [Graphical view]
Pfam	PF04427. Brix. 1 hit. [Graphical view]
SMART	SM00879. Brix. 1 hit. [Graphical view]
SUPFAM	SSF52954. Anticodon_bd. 1 hit.
PROSITE	PS50833. BRIX. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	982188.

Entry information

Entry name

RPF1_YEAST

Accession

Primary (citable) accession number: P38805
Secondary accession number(s): D3DL40

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1995
Last sequence update:	February 1, 1995
Last modified:	April 3, 2013
This is version 94 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program

Relevant documents

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

Yeast chromosome VIII

Yeast (Saccharomyces cerevisiae) chromosome VIII: entries and gene names

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents