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Protein

Ribosome production factor 1

Gene

RPF1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Essential protein. Required for biogenesis of the 60S ribosomal subunit.2 Publications

GO - Molecular functioni

  • rRNA primary transcript binding Source: SGD

GO - Biological processi

  • maturation of 5.8S rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) Source: SGD
  • maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) Source: SGD
  • ribosomal large subunit assembly Source: SGD
  • ribosomal large subunit export from nucleus Source: SGD
Complete GO annotation...

Keywords - Biological processi

Ribosome biogenesis, rRNA processing

Keywords - Ligandi

RNA-binding, rRNA-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-31135-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Ribosome production factor 1
Alternative name(s):
Ribosome biogenesis protein RPF1
Gene namesi
Name:RPF1
Ordered Locus Names:YHR088W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311 Componenti: Chromosome VIII

Organism-specific databases

CYGDiYHR088w.
EuPathDBiFungiDB:YHR088W.
SGDiS000001130. RPF1.

Subcellular locationi

GO - Cellular componenti

  • nucleolus Source: SGD
  • preribosome, large subunit precursor Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 295295Ribosome production factor 1PRO_0000120255Add
BLAST

Proteomic databases

MaxQBiP38805.
PaxDbiP38805.

Expressioni

Gene expression databases

GenevestigatoriP38805.

Interactioni

Subunit structurei

Part of a complex that includes BRX1, RPF1, RPF2 and SSF1 or SSF2.

Protein-protein interaction databases

BioGridi36523. 83 interactions.
DIPiDIP-6511N.
IntActiP38805. 26 interactions.
MINTiMINT-8285251.
STRINGi4932.YHR088W.

Structurei

3D structure databases

ProteinModelPortaliP38805.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini93 – 276184BrixPROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni254 – 27118RNA-bindingAdd
BLAST

Sequence similaritiesi

Contains 1 Brix domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiCOG2136.
GeneTreeiENSGT00550000074691.
HOGENOMiHOG000172062.
InParanoidiP38805.
KOiK14846.
OMAiYEWIHKR.
OrthoDBiEOG757D7K.

Family and domain databases

Gene3Di3.40.50.10480. 1 hit.
InterProiIPR004154. Anticodon-bd.
IPR007109. Brix.
[Graphical view]
PfamiPF04427. Brix. 1 hit.
[Graphical view]
SMARTiSM00879. Brix. 1 hit.
[Graphical view]
SUPFAMiSSF52954. SSF52954. 1 hit.
PROSITEiPS50833. BRIX. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P38805-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MALGNEINIT NKLKRQEIFA DIKHEKNKER HTMRRKRAKE ERENPELREQ
60 70 80 90 100
RLKENVTQTI ENTRVYDETI NKEVEGDEDD LMRYFNSNSN EPPKIFLTTN
110 120 130 140 150
VNAKKSAYEF ANILIEILPN VTFVKRKFGY KLKEISDICI KRNFTDIVII
160 170 180 190 200
NEDKKKVTGL TFIHLPEGPT FYFKLSSFVE VKKIVGHGRP TSHIPELILN
210 220 230 240 250
NFQTRLGQTV GRLFQSILPQ NPDIEGRQVI TLHNQRDYIF FRRHRYVFKD
260 270 280 290
NERVGLQELG PQFTLKLKRL QRGIKEETEW EHKPEMDKEK KKFYL
Length:295
Mass (Da):35,121
Last modified:February 1, 1995 - v1
Checksum:iEC6EDB2FCE252D4A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00060 Genomic DNA. Translation: AAB68926.1.
BK006934 Genomic DNA. Translation: DAA06784.1.
PIRiS46718.
RefSeqiNP_011956.1. NM_001179218.1.

Genome annotation databases

EnsemblFungiiYHR088W; YHR088W; YHR088W.
GeneIDi856488.
KEGGisce:YHR088W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00060 Genomic DNA. Translation: AAB68926.1.
BK006934 Genomic DNA. Translation: DAA06784.1.
PIRiS46718.
RefSeqiNP_011956.1. NM_001179218.1.

3D structure databases

ProteinModelPortaliP38805.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi36523. 83 interactions.
DIPiDIP-6511N.
IntActiP38805. 26 interactions.
MINTiMINT-8285251.
STRINGi4932.YHR088W.

Proteomic databases

MaxQBiP38805.
PaxDbiP38805.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYHR088W; YHR088W; YHR088W.
GeneIDi856488.
KEGGisce:YHR088W.

Organism-specific databases

CYGDiYHR088w.
EuPathDBiFungiDB:YHR088W.
SGDiS000001130. RPF1.

Phylogenomic databases

eggNOGiCOG2136.
GeneTreeiENSGT00550000074691.
HOGENOMiHOG000172062.
InParanoidiP38805.
KOiK14846.
OMAiYEWIHKR.
OrthoDBiEOG757D7K.

Enzyme and pathway databases

BioCyciYEAST:G3O-31135-MONOMER.

Miscellaneous databases

NextBioi982188.
PROiP38805.

Gene expression databases

GenevestigatoriP38805.

Family and domain databases

Gene3Di3.40.50.10480. 1 hit.
InterProiIPR004154. Anticodon-bd.
IPR007109. Brix.
[Graphical view]
PfamiPF04427. Brix. 1 hit.
[Graphical view]
SMARTiSM00879. Brix. 1 hit.
[Graphical view]
SUPFAMiSSF52954. SSF52954. 1 hit.
PROSITEiPS50833. BRIX. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  2. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  3. "The sigma(70)-like motif: a eukaryotic RNA binding domain unique to a superfamily of proteins required for ribosome biogenesis."
    Wehner K.A., Baserga S.J.
    Mol. Cell 9:329-339(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION.
  4. "Functional analysis in yeast of the Brix protein superfamily involved in the biogenesis of ribosomes."
    Bogengruber E., Briza P., Doppler E., Wimmer H., Koller L., Fasiolo F., Senger B., Hegemann J.H., Breitenbach M.
    FEMS Yeast Res. 3:35-43(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION, POSSIBLE COMPLEX COMPOSITION.
  5. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  6. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiRPF1_YEAST
AccessioniPrimary (citable) accession number: P38805
Secondary accession number(s): D3DL40
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: February 1, 1995
Last modified: April 29, 2015
This is version 107 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 784 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome VIII
    Yeast (Saccharomyces cerevisiae) chromosome VIII: entries and gene names

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.