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Protein

Ribosome production factor 1

Gene

RPF1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Essential protein. Required for biogenesis of the 60S ribosomal subunit.2 Publications

Miscellaneous

Present with 784 molecules/cell in log phase SD medium.1 Publication

GO - Molecular functioni

  • rRNA primary transcript binding Source: SGD

GO - Biological processi

  • maturation of 5.8S rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) Source: SGD
  • maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) Source: SGD
  • ribosomal large subunit assembly Source: SGD
  • ribosomal large subunit export from nucleus Source: SGD

Keywordsi

Molecular functionRNA-binding, rRNA-binding
Biological processRibosome biogenesis, rRNA processing

Enzyme and pathway databases

BioCyciYEAST:G3O-31135-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Ribosome production factor 1
Alternative name(s):
Ribosome biogenesis protein RPF1
Gene namesi
Name:RPF1
Ordered Locus Names:YHR088W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome VIII

Organism-specific databases

EuPathDBiFungiDB:YHR088W.
SGDiS000001130. RPF1.

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: EnsemblPlants
  • nucleolus Source: SGD
  • preribosome, large subunit precursor Source: SGD

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001202551 – 295Ribosome production factor 1Add BLAST295

Proteomic databases

MaxQBiP38805.
PRIDEiP38805.
TopDownProteomicsiP38805.

Interactioni

Subunit structurei

Part of a complex that includes BRX1, RPF1, RPF2 and SSF1 or SSF2.

Binary interactionsi

Show more details

Protein-protein interaction databases

BioGridi36523. 249 interactors.
DIPiDIP-6511N.
IntActiP38805. 33 interactors.
MINTiMINT-8285251.

Structurei

3D structure databases

ProteinModelPortaliP38805.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini93 – 276BrixPROSITE-ProRule annotationAdd BLAST184

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni254 – 271RNA-bindingAdd BLAST18

Phylogenomic databases

GeneTreeiENSGT00550000074691.
HOGENOMiHOG000172062.
InParanoidiP38805.
KOiK14846.
OMAiMFQTLFP.
OrthoDBiEOG092C3ODK.

Family and domain databases

InterProiView protein in InterPro
IPR004154. Anticodon-bd.
IPR007109. Brix.
PfamiView protein in Pfam
PF04427. Brix. 1 hit.
SMARTiView protein in SMART
SM00879. Brix. 1 hit.
SUPFAMiSSF52954. SSF52954. 1 hit.
PROSITEiView protein in PROSITE
PS50833. BRIX. 1 hit.

Sequencei

Sequence statusi: Complete.

P38805-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MALGNEINIT NKLKRQEIFA DIKHEKNKER HTMRRKRAKE ERENPELREQ
60 70 80 90 100
RLKENVTQTI ENTRVYDETI NKEVEGDEDD LMRYFNSNSN EPPKIFLTTN
110 120 130 140 150
VNAKKSAYEF ANILIEILPN VTFVKRKFGY KLKEISDICI KRNFTDIVII
160 170 180 190 200
NEDKKKVTGL TFIHLPEGPT FYFKLSSFVE VKKIVGHGRP TSHIPELILN
210 220 230 240 250
NFQTRLGQTV GRLFQSILPQ NPDIEGRQVI TLHNQRDYIF FRRHRYVFKD
260 270 280 290
NERVGLQELG PQFTLKLKRL QRGIKEETEW EHKPEMDKEK KKFYL
Length:295
Mass (Da):35,121
Last modified:February 1, 1995 - v1
Checksum:iEC6EDB2FCE252D4A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00060 Genomic DNA. Translation: AAB68926.1.
BK006934 Genomic DNA. Translation: DAA06784.1.
PIRiS46718.
RefSeqiNP_011956.1. NM_001179218.1.

Genome annotation databases

EnsemblFungiiYHR088W; YHR088W; YHR088W.
GeneIDi856488.
KEGGisce:YHR088W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00060 Genomic DNA. Translation: AAB68926.1.
BK006934 Genomic DNA. Translation: DAA06784.1.
PIRiS46718.
RefSeqiNP_011956.1. NM_001179218.1.

3D structure databases

ProteinModelPortaliP38805.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi36523. 249 interactors.
DIPiDIP-6511N.
IntActiP38805. 33 interactors.
MINTiMINT-8285251.

Proteomic databases

MaxQBiP38805.
PRIDEiP38805.
TopDownProteomicsiP38805.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYHR088W; YHR088W; YHR088W.
GeneIDi856488.
KEGGisce:YHR088W.

Organism-specific databases

EuPathDBiFungiDB:YHR088W.
SGDiS000001130. RPF1.

Phylogenomic databases

GeneTreeiENSGT00550000074691.
HOGENOMiHOG000172062.
InParanoidiP38805.
KOiK14846.
OMAiMFQTLFP.
OrthoDBiEOG092C3ODK.

Enzyme and pathway databases

BioCyciYEAST:G3O-31135-MONOMER.

Miscellaneous databases

PROiPR:P38805.

Family and domain databases

InterProiView protein in InterPro
IPR004154. Anticodon-bd.
IPR007109. Brix.
PfamiView protein in Pfam
PF04427. Brix. 1 hit.
SMARTiView protein in SMART
SM00879. Brix. 1 hit.
SUPFAMiSSF52954. SSF52954. 1 hit.
PROSITEiView protein in PROSITE
PS50833. BRIX. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiRPF1_YEAST
AccessioniPrimary (citable) accession number: P38805
Secondary accession number(s): D3DL40
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: February 1, 1995
Last modified: May 10, 2017
This is version 124 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  2. Yeast chromosome VIII
    Yeast (Saccharomyces cerevisiae) chromosome VIII: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.