P38803 (IPI1_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Pre-rRNA-processing protein IPI1

Alternative name(s):
Involved in processing IST2 protein 1

Gene names

Name:	IPI1
Ordered Locus Names:	YHR085W

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › Saccharomyces cerevisiae

Protein attributes

Sequence length	334 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Component of the RIX1 complex required for processing of ITS2 sequences from 35S pre-rRNA. Ref.6 Ref.7
Subunit structure	Component of the RIX1 complex, composed of at least IPI1, IPI3 and RIX1. The complex interacts with MDN1 and the pre-60S ribosome particles. Ref.3 Ref.6 Ref.7 Ref.8
Subcellular location	Nucleus Ref.4 Ref.6.
Miscellaneous	Present with 4420 molecules/cell in log phase SD medium.
Sequence similarities	Belongs to the IPI1 family.

Ontologies

Keywords
Biological process	Ribosome biogenesis rRNA processing
Cellular component	Nucleus
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	pre-replicative complex assembly Inferred from mutant phenotype PubMed 22421151. Source: SGD rRNA processing Inferred from mutant phenotype PubMed 12089522Ref.7. Source: SGD regulation of DNA-dependent DNA replication initiation Inferred from mutant phenotype PubMed 22421151. Source: SGD ribosomal large subunit assembly Inferred from mutant phenotype Ref.6. Source: SGD
Cellular_component	Rix1 complex Inferred from direct assay Ref.7. Source: SGD nucleoplasm Inferred from direct assay Ref.6. Source: SGD
Molecular_function	chromatin binding Inferred from direct assay PubMed 22421151. Source: SGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 334	334	Pre-rRNA-processing protein IPI1		PRO_0000202904

Sequences

	Sequence		Length	Mass (Da)
	P38803 [UniParc]. Last modified February 1, 1995. Version 1. Checksum: 1AB0B318BBFC8096	FASTA	334	37,867
	10 20 30 40 50 60 MTKSRKQKQK KQDFLRKKLK VGKPKEKARN ATDTSFVSKT ISIRNQHLDQ NPHDLTKRLT 70 80 90 100 110 120 LLKHHNINVR KETLTTFQKS IPSIIKSRLM TPLLTQSIPL ICDESQQVRQ GLIDLVDEIG 130 140 150 160 170 180 SHDAEILKLH CNIFVLYINM AMTHIVTQIQ ADSTKFLSHL LKYCGDEVVR KSWVKLLNGV 190 200 210 220 230 240 FGVLGWGQVG KNDSASIVQT KKRNAKYVTI HLNALYTLVE YGCQDERARS DGDTAETTED 250 260 270 280 290 300 SGTLRNPYLI PDYPQPFEHL KLFTRELKVQ DATSSGVNAT LLSLATQDID TRKAVFIEQF 310 320 330 LPIVRKKIEV IIKEGGECGK SANKLKTLLA KIFD

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Complete nucleotide sequence of Saccharomyces cerevisiae chromosome VIII." Johnston M., Andrews S., Brinkman R., Cooper J., Ding H., Dover J., Du Z., Favello A., Fulton L., Gattung S., Geisel C., Kirsten J., Kucaba T., Hillier L.W., Jier M., Johnston L., Langston Y., Latreille P. , Louis E.J., Macri C., Mardis E., Menezes S., Mouser L., Nhan M., Rifkin L., Riles L., St Peter H., Trevaskis E., Vaughan K., Vignati D., Wilcox L., Wohldman P., Waterston R., Wilson R., Vaudin M. Science 265:2077-2082(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[2]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[3]	"Assigning function to yeast proteins by integration of technologies." Hazbun T.R., Malmstroem L., Anderson S., Graczyk B.J., Fox B., Riffle M., Sundin B.A., Aranda J.D., McDonald W.H., Chiu C.-H., Snydsman B.E., Bradley P., Muller E.G.D., Fields S., Baker D., Yates J.R. III, Davis T.N. Mol. Cell 12:1353-1365(2003) [PubMed] [Europe PMC] [Abstract] Cited for: MASS SPECTROMETRY, IDENTIFICATION IN THE RIX1 COMPLEX.
[4]	"Global analysis of protein localization in budding yeast." Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K. Nature 425:686-691(2003) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[5]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[6]	"Rea1, a dynein-related nuclear AAA-ATPase, is involved in late rRNA processing and nuclear export of 60 S subunits." Galani K., Nissan T.A., Petfalski E., Tollervey D., Hurt E. J. Biol. Chem. 279:55411-55418(2004) [PubMed] [Europe PMC] [Abstract] Cited for: MASS SPECTROMETRY, IDENTIFICATION IN THE RIX1 COMPLEX, SUBCELLULAR LOCATION, FUNCTION OF THE RIX1 COMPLEX.
[7]	"High-definition macromolecular composition of yeast RNA-processing complexes." Krogan N.J., Peng W.-T., Cagney G., Robinson M.D., Haw R., Zhong G., Guo X., Zhang X., Canadien V., Richards D.P., Beattie B.K., Lalev A., Zhang W., Davierwala A.P., Mnaimneh S., Starostine A., Tikuisis A.P., Grigull J. , Datta N., Bray J.E., Hughes T.R., Emili A., Greenblatt J.F. Mol. Cell 13:225-239(2004) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION IN THE RIX1 COMPLEX, FUNCTION OF THE RIX1 COMPLEX.
[8]	"A pre-ribosome with a tadpole-like structure functions in ATP-dependent maturation of 60S subunits." Nissan T.A., Galani K., Maco B., Tollervey D., Aebi U., Hurt E. Mol. Cell 15:295-301(2004) [PubMed] [Europe PMC] [Abstract] Cited for: MASS SPECTROMETRY, IDENTIFICATION IN THE RIX1 COMPLEX, INTERACTION WITH MDN1 AND PRE-60S RIBOSOMAL PARTICLES.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U10556 Genomic DNA. Translation: AAB68883.1. BK006934 Genomic DNA. Translation: DAA06780.1.
PIR	S46805.
RefSeq	NP_011953.1. NM_001179215.1.
3D structure databases
ProteinModelPortal	P38803.
SMR	P38803. Positions 103-161.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-4665N.
IntAct	P38803. 28 interactions.
MINT	MINT-481995.
STRING	4932.YHR085W.
Proteomic databases
PaxDb	P38803.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YHR085W; YHR085W; YHR085W.
GeneID	856485.
KEGG	sce:YHR085W.
Organism-specific databases
CYGD	YHR085w.
SGD	S000001127. IPI1.
Phylogenomic databases
eggNOG	NOG315171.
GeneTree	ENSGT00390000012654.
HOGENOM	HOG000094409.
KO	K14827.
OMA	LSLTKHH.
OrthoDB	EOG4H49DM.
Gene expression databases
Genevestigator	P38803.
GermOnline	YHR085W. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR016024. ARM-type_fold. IPR024679. IPI1/TEX10. [Graphical view]
Pfam	PF12333. Ipi1_N. 1 hit. [Graphical view]
SUPFAM	SSF48371. ARM-type_fold. 1 hit.
ProtoNet	Search...
Other
NextBio	982179.

Entry information

Entry name

IPI1_YEAST

Accession

Primary (citable) accession number: P38803
Secondary accession number(s): D3DL36

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1995
Last sequence update:	February 1, 1995
Last modified:	May 1, 2013
This is version 84 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program