Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

P38798

- NMD2_YEAST

UniProt

P38798 - NMD2_YEAST

Protein

Nonsense-mediated mRNA decay protein 2

Gene

NMD2

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 118 (01 Oct 2014)
      Sequence version 2 (01 Nov 1995)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Involved in nonsense-mediated decay of mRNAs containing premature stop codons. It interacts, via its C-terminus, with NAM7/UPF1. Could be involved in determining the efficiency of translational termination or reinitiation or factors involved in the initial assembly of an initiation- and termination-competent mRNP.

    GO - Molecular functioni

    1. RNA binding Source: InterPro

    GO - Biological processi

    1. DNA recombination Source: SGD
    2. nuclear-transcribed mRNA catabolic process, 3'-5' exonucleolytic nonsense-mediated decay Source: SGD

    Enzyme and pathway databases

    BioCyciYEAST:G3O-31124-MONOMER.
    ReactomeiREACT_189050. Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC).

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Nonsense-mediated mRNA decay protein 2
    Alternative name(s):
    Up-frameshift suppressor 2
    Gene namesi
    Name:NMD2
    Synonyms:IFS1, SUA1, UPF2
    Ordered Locus Names:YHR077C
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome VIII

    Organism-specific databases

    CYGDiYHR077c.
    SGDiS000001119. NMD2.

    Subcellular locationi

    Cytoplasm Curated

    GO - Cellular componenti

    1. cytoplasm Source: SGD
    2. polysome Source: SGD

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 10891089Nonsense-mediated mRNA decay protein 2PRO_0000096873Add
    BLAST

    Proteomic databases

    MaxQBiP38798.
    PaxDbiP38798.

    Expressioni

    Gene expression databases

    GenevestigatoriP38798.

    Interactioni

    Protein-protein interaction databases

    BioGridi36511. 160 interactions.
    DIPiDIP-2295N.
    IntActiP38798. 4 interactions.
    MINTiMINT-1521719.
    STRINGi4932.YHR077C.

    Structurei

    3D structure databases

    ProteinModelPortaliP38798.
    SMRiP38798. Positions 3-311, 575-780.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi843 – 975133Asp/Glu-rich (highly acidic)Add
    BLAST

    Phylogenomic databases

    eggNOGiNOG321770.
    GeneTreeiENSGT00530000064318.
    KOiK14327.
    OMAiIRKLHWE.
    OrthoDBiEOG7TJ3S6.

    Family and domain databases

    Gene3Di1.25.40.180. 2 hits.
    InterProiIPR016024. ARM-type_fold.
    IPR016021. MIF4-like_typ_1/2/3.
    IPR003890. MIF4G-like_typ-3.
    IPR007193. Up-fram_suppressor-2.
    [Graphical view]
    PfamiPF02854. MIF4G. 3 hits.
    PF04050. Upf2. 1 hit.
    [Graphical view]
    SMARTiSM00543. MIF4G. 3 hits.
    [Graphical view]
    SUPFAMiSSF48371. SSF48371. 2 hits.

    Sequencei

    Sequence statusi: Complete.

    P38798-1 [UniParc]FASTAAdd to Basket

    « Hide

    MDDGRKKELH DLNTRAWNGE EVFPLKSKKL DSSIKRNTGF IKKLKKGFVK     50
    GSESSLLKDL SEASLEKYLS EIIVTVTECL LNVLNKNDDV IAAVEIISGL 100
    HQRFNGRFTS PLLGAFLQAF ENPSVDIESE RDELQRITRV KGNLRVFTEL 150
    YLVGVFRTLD DIESKDAIPN FLQKKTGRKD PLLFSILREI LNYKFKLGFT 200
    TTIATAFIKK FAPLFRDDDN SWDDLIYDSK LKGALQSLFK NFIDATFARA 250
    TELHKKVNKL QREHQKCQIR TGKLRDEYVE EYDKLLPIFI RFKTSAITLG 300
    EFFKLEIPEL EGASNDDLKE TASPMITNQI LPPNQRLWEN EDTRKFYEIL 350
    PDISKTVEES QSSKTEKDSN VNSKNINLFF TDLEMADCKD IIDDLSNRYW 400
    SSYLDNKATR NRILKFFMET QDWSKLPVYS RFIATNSKYM PEIVSEFINY 450
    LDNGFRSQLH SNKINVKNII FFSEMIKFQL IPSFMIFHKI RTLIMYMQVP 500
    NNVEILTVLL EHSGKFLLNK PEYKELMEKM VQLIKDKKND RQLNMNMKSA 550
    LENIITLLYP PSVKSLNVTV KTITPEQQFY RILIRSELSS LDFKHIVKLV 600
    RKAHWDDVAI QKVLFSLFSK PHKISYQNIP LLTKVLGGLY SYRRDFVIRC 650
    IDQVLENIER GLEINDYGQN MHRISNVRYL TEIFNFEMIK SDVLLDTIYH 700
    IIRFGHINNQ PNPFYLNYSD PPDNYFRIQL VTTILLNINR TPAAFTKKCK 750
    LLLRFFEYYT FIKEQPLPKE TEFRVSSTFK KYENIFGNTK FERSENLVES 800
    ASRLESLLKS LNAIKSKDDR VKGSSASIHN GKESAVPIES ITEDDEDEDD 850
    ENDDGVDLLG EDEDAEISTP NTESAPGKHQ AKQDESEDED DEDDDEDDDD 900
    DDDDDDDDGE EGDEDDDEDD DDEDDDDEEE EDSDSDLEYG GDLDADRDIE 950
    MKRMYEEYER KLKDEEERKA EEELERQFQK MMQESIDARK SEKVVASKIP 1000
    VISKPVSVQK PLLLKKSEEP SSSKETYEEL SKPKKIAFTF LTKSGKKTQS 1050
    RILQLPTDVK FVSDVLEEEE KLKTERNKIK KIVLKRSFD 1089
    Length:1,089
    Mass (Da):126,747
    Last modified:November 1, 1995 - v2
    Checksum:i13BBE725675CBF52
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti2 – 21D → YQQ(PubMed:7604038)Curated
    Sequence conflicti2 – 21D → YQQ(PubMed:8091229)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U14974 Genomic DNA. Translation: AAA67724.1.
    U12137 Genomic DNA. Translation: AAA66521.1.
    U28158 Genomic DNA. Translation: AAA74948.1.
    U10556 Genomic DNA. Translation: AAB68893.1.
    BK006934 Genomic DNA. Translation: DAA06771.1.
    PIRiS48244.
    RefSeqiNP_011944.2. NM_001179207.1.

    Genome annotation databases

    EnsemblFungiiYHR077C; YHR077C; YHR077C.
    GeneIDi856476.
    KEGGisce:YHR077C.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U14974 Genomic DNA. Translation: AAA67724.1 .
    U12137 Genomic DNA. Translation: AAA66521.1 .
    U28158 Genomic DNA. Translation: AAA74948.1 .
    U10556 Genomic DNA. Translation: AAB68893.1 .
    BK006934 Genomic DNA. Translation: DAA06771.1 .
    PIRi S48244.
    RefSeqi NP_011944.2. NM_001179207.1.

    3D structure databases

    ProteinModelPortali P38798.
    SMRi P38798. Positions 3-311, 575-780.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 36511. 160 interactions.
    DIPi DIP-2295N.
    IntActi P38798. 4 interactions.
    MINTi MINT-1521719.
    STRINGi 4932.YHR077C.

    Proteomic databases

    MaxQBi P38798.
    PaxDbi P38798.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YHR077C ; YHR077C ; YHR077C .
    GeneIDi 856476.
    KEGGi sce:YHR077C.

    Organism-specific databases

    CYGDi YHR077c.
    SGDi S000001119. NMD2.

    Phylogenomic databases

    eggNOGi NOG321770.
    GeneTreei ENSGT00530000064318.
    KOi K14327.
    OMAi IRKLHWE.
    OrthoDBi EOG7TJ3S6.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-31124-MONOMER.
    Reactomei REACT_189050. Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC).

    Miscellaneous databases

    NextBioi 982152.
    PROi P38798.

    Gene expression databases

    Genevestigatori P38798.

    Family and domain databases

    Gene3Di 1.25.40.180. 2 hits.
    InterProi IPR016024. ARM-type_fold.
    IPR016021. MIF4-like_typ_1/2/3.
    IPR003890. MIF4G-like_typ-3.
    IPR007193. Up-fram_suppressor-2.
    [Graphical view ]
    Pfami PF02854. MIF4G. 3 hits.
    PF04050. Upf2. 1 hit.
    [Graphical view ]
    SMARTi SM00543. MIF4G. 3 hits.
    [Graphical view ]
    SUPFAMi SSF48371. SSF48371. 2 hits.
    ProtoNeti Search...

    Publicationsi

    1. "Identification of a novel component of the nonsense-mediated mRNA decay pathway by use of an interacting protein screen."
      He F., Jacobson A.
      Genes Dev. 9:437-454(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. "Identification and characterization of genes that are required for the accelerated degradation of mRNAs containing a premature translational termination codon."
      Cui Y., Hagan K.W., Zhang S., Peltz S.W.
      Genes Dev. 9:423-436(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: PLY136.
    3. "A genetic screen identifies cellular factors involved in retroviral -1 frameshifting."
      Lee S.I., Umen J.G., Varmus H.E.
      Proc. Natl. Acad. Sci. U.S.A. 92:6587-6591(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    5. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    6. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiNMD2_YEAST
    AccessioniPrimary (citable) accession number: P38798
    Secondary accession number(s): D3DL27
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 1, 1995
    Last sequence update: November 1, 1995
    Last modified: October 1, 2014
    This is version 118 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 1280 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    2. Yeast chromosome VIII
      Yeast (Saccharomyces cerevisiae) chromosome VIII: entries and gene names

    External Data

    Dasty 3