Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

P38798 (NMD2_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified March 19, 2014. Version 113. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Nonsense-mediated mRNA decay protein 2
Alternative name(s):
Up-frameshift suppressor 2
Gene names
Name:NMD2
Synonyms:IFS1, SUA1, UPF2
Ordered Locus Names:YHR077C
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length1089 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Involved in nonsense-mediated decay of mRNAs containing premature stop codons. It interacts, via its C-terminus, with NAM7/UPF1. Could be involved in determining the efficiency of translational termination or reinitiation or factors involved in the initial assembly of an initiation- and termination-competent mRNP.

Subcellular location

Cytoplasm Potential.

Miscellaneous

Present with 1280 molecules/cell in log phase SD medium.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 10891089Nonsense-mediated mRNA decay protein 2
PRO_0000096873

Regions

Compositional bias843 – 975133Asp/Glu-rich (highly acidic)

Experimental info

Sequence conflict21D → YQQ Ref.3
Sequence conflict21D → YQQ Ref.4

Sequences

Sequence LengthMass (Da)Tools
P38798 [UniParc].

Last modified November 1, 1995. Version 2.
Checksum: 13BBE725675CBF52

FASTA1,089126,747
        10         20         30         40         50         60 
MDDGRKKELH DLNTRAWNGE EVFPLKSKKL DSSIKRNTGF IKKLKKGFVK GSESSLLKDL 

        70         80         90        100        110        120 
SEASLEKYLS EIIVTVTECL LNVLNKNDDV IAAVEIISGL HQRFNGRFTS PLLGAFLQAF 

       130        140        150        160        170        180 
ENPSVDIESE RDELQRITRV KGNLRVFTEL YLVGVFRTLD DIESKDAIPN FLQKKTGRKD 

       190        200        210        220        230        240 
PLLFSILREI LNYKFKLGFT TTIATAFIKK FAPLFRDDDN SWDDLIYDSK LKGALQSLFK 

       250        260        270        280        290        300 
NFIDATFARA TELHKKVNKL QREHQKCQIR TGKLRDEYVE EYDKLLPIFI RFKTSAITLG 

       310        320        330        340        350        360 
EFFKLEIPEL EGASNDDLKE TASPMITNQI LPPNQRLWEN EDTRKFYEIL PDISKTVEES 

       370        380        390        400        410        420 
QSSKTEKDSN VNSKNINLFF TDLEMADCKD IIDDLSNRYW SSYLDNKATR NRILKFFMET 

       430        440        450        460        470        480 
QDWSKLPVYS RFIATNSKYM PEIVSEFINY LDNGFRSQLH SNKINVKNII FFSEMIKFQL 

       490        500        510        520        530        540 
IPSFMIFHKI RTLIMYMQVP NNVEILTVLL EHSGKFLLNK PEYKELMEKM VQLIKDKKND 

       550        560        570        580        590        600 
RQLNMNMKSA LENIITLLYP PSVKSLNVTV KTITPEQQFY RILIRSELSS LDFKHIVKLV 

       610        620        630        640        650        660 
RKAHWDDVAI QKVLFSLFSK PHKISYQNIP LLTKVLGGLY SYRRDFVIRC IDQVLENIER 

       670        680        690        700        710        720 
GLEINDYGQN MHRISNVRYL TEIFNFEMIK SDVLLDTIYH IIRFGHINNQ PNPFYLNYSD 

       730        740        750        760        770        780 
PPDNYFRIQL VTTILLNINR TPAAFTKKCK LLLRFFEYYT FIKEQPLPKE TEFRVSSTFK 

       790        800        810        820        830        840 
KYENIFGNTK FERSENLVES ASRLESLLKS LNAIKSKDDR VKGSSASIHN GKESAVPIES 

       850        860        870        880        890        900 
ITEDDEDEDD ENDDGVDLLG EDEDAEISTP NTESAPGKHQ AKQDESEDED DEDDDEDDDD 

       910        920        930        940        950        960 
DDDDDDDDGE EGDEDDDEDD DDEDDDDEEE EDSDSDLEYG GDLDADRDIE MKRMYEEYER 

       970        980        990       1000       1010       1020 
KLKDEEERKA EEELERQFQK MMQESIDARK SEKVVASKIP VISKPVSVQK PLLLKKSEEP 

      1030       1040       1050       1060       1070       1080 
SSSKETYEEL SKPKKIAFTF LTKSGKKTQS RILQLPTDVK FVSDVLEEEE KLKTERNKIK 


KIVLKRSFD 

« Hide

References

« Hide 'large scale' references
[1]"Identification of a novel component of the nonsense-mediated mRNA decay pathway by use of an interacting protein screen."
He F., Jacobson A.
Genes Dev. 9:437-454(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"Identification and characterization of genes that are required for the accelerated degradation of mRNAs containing a premature translational termination codon."
Cui Y., Hagan K.W., Zhang S., Peltz S.W.
Genes Dev. 9:423-436(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: PLY136.
[3]"A genetic screen identifies cellular factors involved in retroviral -1 frameshifting."
Lee S.I., Umen J.G., Varmus H.E.
Proc. Natl. Acad. Sci. U.S.A. 92:6587-6591(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[4]"Complete nucleotide sequence of Saccharomyces cerevisiae chromosome VIII."
Johnston M., Andrews S., Brinkman R., Cooper J., Ding H., Dover J., Du Z., Favello A., Fulton L., Gattung S., Geisel C., Kirsten J., Kucaba T., Hillier L.W., Jier M., Johnston L., Langston Y., Latreille P. expand/collapse author list , Louis E.J., Macri C., Mardis E., Menezes S., Mouser L., Nhan M., Rifkin L., Riles L., St Peter H., Trevaskis E., Vaughan K., Vignati D., Wilcox L., Wohldman P., Waterston R., Wilson R., Vaudin M.
Science 265:2077-2082(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[5]Saccharomyces Genome Database
Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[6]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U14974 Genomic DNA. Translation: AAA67724.1.
U12137 Genomic DNA. Translation: AAA66521.1.
U28158 Genomic DNA. Translation: AAA74948.1.
U10556 Genomic DNA. Translation: AAB68893.1.
BK006934 Genomic DNA. Translation: DAA06771.1.
PIRS48244.
RefSeqNP_011944.2. NM_001179207.1.

3D structure databases

ProteinModelPortalP38798.
SMRP38798. Positions 573-812.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid36511. 160 interactions.
DIPDIP-2295N.
IntActP38798. 4 interactions.
MINTMINT-1521719.
STRING4932.YHR077C.

Proteomic databases

PaxDbP38798.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYHR077C; YHR077C; YHR077C.
GeneID856476.
KEGGsce:YHR077C.

Organism-specific databases

CYGDYHR077c.
SGDS000001119. NMD2.

Phylogenomic databases

eggNOGNOG321770.
GeneTreeENSGT00530000064318.
KOK14327.
OMAYLDNGFR.
OrthoDBEOG7TJ3S6.

Enzyme and pathway databases

BioCycYEAST:G3O-31124-MONOMER.

Gene expression databases

GenevestigatorP38798.

Family and domain databases

Gene3D1.25.40.180. 2 hits.
InterProIPR016024. ARM-type_fold.
IPR016021. MIF4-like_typ_1/2/3.
IPR003890. MIF4G-like_typ-3.
IPR007193. Up-fram_suppressor-2.
[Graphical view]
PfamPF02854. MIF4G. 3 hits.
PF04050. Upf2. 1 hit.
[Graphical view]
SMARTSM00543. MIF4G. 3 hits.
[Graphical view]
SUPFAMSSF48371. SSF48371. 2 hits.
ProtoNetSearch...

Other

NextBio982152.
PROP38798.

Entry information

Entry nameNMD2_YEAST
AccessionPrimary (citable) accession number: P38798
Secondary accession number(s): D3DL27
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: November 1, 1995
Last modified: March 19, 2014
This is version 113 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome VIII

Yeast (Saccharomyces cerevisiae) chromosome VIII: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD