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Protein

Protein phosphatase methylesterase 1

Gene

PPE1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Demethylates proteins that have been reversibly carboxymethylated. Demethylates the phosphatase PP2A catalytic subunits PPH21 and PPH22. Forms inactive complexes (PP2Ai) with phosphatase PP2A-like catalytic subunits. Involved in the regulation of cell cycle progression at START.3 Publications

Catalytic activityi

[Phosphatase 2A protein]-leucine methyl ester + H2O = [phosphatase 2A protein]-leucine + methanol.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei205 – 2051By similarity
Active sitei233 – 2331By similarity
Active sitei359 – 3591By similarity

GO - Molecular functioni

  • carboxylic ester hydrolase activity Source: SGD
  • structural constituent of ribosome Source: SGD

GO - Biological processi

  • C-terminal protein demethylation Source: SGD
  • mitochondrial translation Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Serine esterase

Enzyme and pathway databases

BioCyciYEAST:YHR075C-MONOMER.

Protein family/group databases

ESTHERiyeast-ppme1. PPase_methylesterase_euk.

Names & Taxonomyi

Protein namesi
Recommended name:
Protein phosphatase methylesterase 1 (EC:3.1.1.89)
Short name:
PME-1
Alternative name(s):
Yms2
Gene namesi
Name:PPE1
Ordered Locus Names:YHR075C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome VIII

Organism-specific databases

EuPathDBiFungiDB:YHR075C.
SGDiS000001117. PPE1.

Subcellular locationi

GO - Cellular componenti

  • mitochondrial small ribosomal subunit Source: SGD
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 400400Protein phosphatase methylesterase 1PRO_0000030588Add
BLAST

Proteomic databases

MaxQBiP38796.
PeptideAtlasiP38796.

Interactioni

Subunit structurei

Interacts with and inactivates the phosphatase PP2A-like catalytic subunits PPG1, PPH21, PPH22, PPH3 AND SIT4.1 Publication

Protein-protein interaction databases

BioGridi36509. 34 interactions.
DIPiDIP-1943N.
IntActiP38796. 2 interactions.
MINTiMINT-410794.

Structurei

3D structure databases

ProteinModelPortaliP38796.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the AB hydrolase superfamily.Curated

Phylogenomic databases

GeneTreeiENSGT00390000004396.
HOGENOMiHOG000212435.
InParanoidiP38796.
KOiK13617.
OMAiLYTWRIE.
OrthoDBiEOG7WX0JP.

Family and domain databases

Gene3Di3.40.50.1820. 1 hit.
InterProiIPR029058. AB_hydrolase.
IPR000073. AB_hydrolase_1.
IPR016812. PPase_methylesterase_euk.
[Graphical view]
PANTHERiPTHR14189. PTHR14189. 1 hit.
PfamiPF00561. Abhydrolase_1. 1 hit.
[Graphical view]
PIRSFiPIRSF022950. PPase_methylesterase_euk. 1 hit.
SUPFAMiSSF53474. SSF53474. 1 hit.

Sequencei

Sequence statusi: Complete.

P38796-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSDDLRRKIA LSQFERAKNV LDATFQEAYE DDENDGDALG SLPSFNGQSN
60 70 80 90 100
RNRKYTGKTG STTDRISSKE KSSLPTWSDF FDNKELVSLP DRDLDVNTYY
110 120 130 140 150
TLPTSLLSNT TSIPIFIFHH GAGSSGLSFA NLAKELNTKL EGRCGCFAFD
160 170 180 190 200
ARGHAETKFK KADAPICFDR DSFIKDFVSL LNYWFKSKIS QEPLQKVSVI
210 220 230 240 250
LIGHSLGGSI CTFAYPKLST ELQKKILGIT MLDIVEEAAI MALNKVEHFL
260 270 280 290 300
QNTPNVFESI NDAVDWHVQH ALSRLRSSAE IAIPALFAPL KSGKVVRITN
310 320 330 340 350
LKTFSPFWDT WFTDLSHSFV GLPVSKLLIL AGNENLDKEL IVGQMQGKYQ
360 370 380 390 400
LVVFQDSGHF IQEDSPIKTA ITLIDFWKRN DSRNVVIKTN WGQHKTVQNT
Length:400
Mass (Da):44,888
Last modified:February 1, 1995 - v1
Checksum:iAD00BEA4FB7795EE
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti36 – 361G → D AA sequence (PubMed:9151978).Curated
Sequence conflicti39 – 391L → P AA sequence (PubMed:9151978).Curated
Sequence conflicti44 – 441S → K AA sequence (PubMed:9151978).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U10556 Genomic DNA. Translation: AAB68892.1.
BK006934 Genomic DNA. Translation: DAA06769.1.
PIRiS46814.
RefSeqiNP_011942.1. NM_001179205.1.

Genome annotation databases

EnsemblFungiiYHR075C; YHR075C; YHR075C.
GeneIDi856474.
KEGGisce:YHR075C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U10556 Genomic DNA. Translation: AAB68892.1.
BK006934 Genomic DNA. Translation: DAA06769.1.
PIRiS46814.
RefSeqiNP_011942.1. NM_001179205.1.

3D structure databases

ProteinModelPortaliP38796.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi36509. 34 interactions.
DIPiDIP-1943N.
IntActiP38796. 2 interactions.
MINTiMINT-410794.

Protein family/group databases

ESTHERiyeast-ppme1. PPase_methylesterase_euk.

Proteomic databases

MaxQBiP38796.
PeptideAtlasiP38796.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYHR075C; YHR075C; YHR075C.
GeneIDi856474.
KEGGisce:YHR075C.

Organism-specific databases

EuPathDBiFungiDB:YHR075C.
SGDiS000001117. PPE1.

Phylogenomic databases

GeneTreeiENSGT00390000004396.
HOGENOMiHOG000212435.
InParanoidiP38796.
KOiK13617.
OMAiLYTWRIE.
OrthoDBiEOG7WX0JP.

Enzyme and pathway databases

BioCyciYEAST:YHR075C-MONOMER.

Miscellaneous databases

NextBioi982146.
PROiP38796.

Family and domain databases

Gene3Di3.40.50.1820. 1 hit.
InterProiIPR029058. AB_hydrolase.
IPR000073. AB_hydrolase_1.
IPR016812. PPase_methylesterase_euk.
[Graphical view]
PANTHERiPTHR14189. PTHR14189. 1 hit.
PfamiPF00561. Abhydrolase_1. 1 hit.
[Graphical view]
PIRSFiPIRSF022950. PPase_methylesterase_euk. 1 hit.
SUPFAMiSSF53474. SSF53474. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  2. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  3. "Identification and characterization of the genes for mitochondrial ribosomal proteins of Saccharomyces cerevisiae."
    Kitakawa M., Graack H.-R., Grohmann L., Goldschmidt-Reisin S., Herfurth E., Wittmann-Liebold B., Nishimura T., Isono K.
    Eur. J. Biochem. 245:449-456(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 36-44.
    Strain: S288c / DC-5A.
  4. "Carboxyl methylation of the phosphoprotein phosphatase 2A catalytic subunit promotes its functional association with regulatory subunits in vivo."
    Wu J., Tolstykh T., Lee J., Boyd K., Stock J.B., Broach J.R.
    EMBO J. 19:5672-5681(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  5. "A new enrichment approach identifies genes that alter cell cycle progression in Saccharomyces cerevisiae."
    Bogomolnaya L.M., Pathak R., Cham R., Guo J., Surovtseva Y.V., Jaeckel L., Polymenis M.
    Curr. Genet. 45:350-359(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  6. "Synergistic computational and experimental proteomics approaches for more accurate detection of active serine hydrolases in yeast."
    Baxter S.M., Rosenblum J.S., Knutson S., Nelson M.R., Montimurro J.S., Di Gennaro J.A., Speir J.A., Burbaum J.J., Fetrow J.S.
    Mol. Cell. Proteomics 3:209-225(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION AS A SERINE HYDROLASE, IDENTIFICATION BY MASS SPECTROMETRY.
  7. "Specific interactions of PP2A and PP2A-like phosphatases with the yeast PTPA homologues, Ypa1 and Ypa2."
    Van Hoof C., Martens E., Longin S., Jordens J., Stevens I., Janssens V., Goris J.
    Biochem. J. 386:93-102(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH PPG1; PPH21; PPH22; PPH3 AND SIT4.

Entry informationi

Entry nameiPPME1_YEAST
AccessioniPrimary (citable) accession number: P38796
Secondary accession number(s): D3DL25
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: February 1, 1995
Last modified: May 11, 2016
This is version 133 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Caution

Was originally thought to be a mitochondrial ribosomal protein.1 Publication

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome VIII
    Yeast (Saccharomyces cerevisiae) chromosome VIII: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.