P38796 (PPME1_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Protein phosphatase methylesterase 1
Short name=PME-1
EC=3.1.1.89

Alternative name(s):
Yms2

Gene names

Name:	PPE1
Ordered Locus Names:	YHR075C

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › Saccharomyces cerevisiae

Protein attributes

Sequence length	400 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Demethylates proteins that have been reversibly carboxymethylated. Demethylates the phosphatase PP2A catalytic subunits PPH21 and PPH22. Forms inactive complexes (PP2Ai) with phosphatase PP2A-like catalytic subunits. Involved in the regulation of cell cycle progression at START. Ref.4 Ref.5 Ref.7
Catalytic activity	[Phosphatase 2A protein]-leucine methyl ester + H2O = [phosphatase 2A protein]-leucine + methanol.
Subunit structure	Interacts with and inactivates the phosphatase PP2A-like catalytic subunits PPG1, PPH21, PPH22, PPH3 AND SIT4. Ref.7
Sequence similarities	Belongs to the AB hydrolase superfamily.
Caution	Was originally (Ref.3) thought to be a mitochondrial ribosomal protein.

Ontologies

Keywords
Molecular function	Hydrolase Serine esterase
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	cellular protein modification process Inferred from genetic interaction Ref.4. Source: SGD mitochondrial translation Inferred by curator Ref.3. Source: SGD
Cellular_component	mitochondrial small ribosomal subunit Inferred from direct assay Ref.3. Source: SGD
Molecular_function	carboxylesterase activity Inferred from direct assay Ref.4. Source: SGD structural constituent of ribosome Inferred from direct assay Ref.3. Source: SGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 400	400	Protein phosphatase methylesterase 1		PRO_0000030588
Sites
	Active site	205	1	By similarity
	Active site	233	1	By similarity
	Active site	359	1	By similarity
Experimental info
	Sequence conflict	36	1	G → D AA sequence Ref.3
	Sequence conflict	39	1	L → P AA sequence Ref.3
	Sequence conflict	44	1	S → K AA sequence Ref.3

Sequences

	Sequence		Length	Mass (Da)
	P38796 [UniParc]. Last modified February 1, 1995. Version 1. Checksum: AD00BEA4FB7795EE	FASTA	400	44,888
	10 20 30 40 50 60 MSDDLRRKIA LSQFERAKNV LDATFQEAYE DDENDGDALG SLPSFNGQSN RNRKYTGKTG 70 80 90 100 110 120 STTDRISSKE KSSLPTWSDF FDNKELVSLP DRDLDVNTYY TLPTSLLSNT TSIPIFIFHH 130 140 150 160 170 180 GAGSSGLSFA NLAKELNTKL EGRCGCFAFD ARGHAETKFK KADAPICFDR DSFIKDFVSL 190 200 210 220 230 240 LNYWFKSKIS QEPLQKVSVI LIGHSLGGSI CTFAYPKLST ELQKKILGIT MLDIVEEAAI 250 260 270 280 290 300 MALNKVEHFL QNTPNVFESI NDAVDWHVQH ALSRLRSSAE IAIPALFAPL KSGKVVRITN 310 320 330 340 350 360 LKTFSPFWDT WFTDLSHSFV GLPVSKLLIL AGNENLDKEL IVGQMQGKYQ LVVFQDSGHF 370 380 390 400 IQEDSPIKTA ITLIDFWKRN DSRNVVIKTN WGQHKTVQNT

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Complete nucleotide sequence of Saccharomyces cerevisiae chromosome VIII." Johnston M., Andrews S., Brinkman R., Cooper J., Ding H., Dover J., Du Z., Favello A., Fulton L., Gattung S., Geisel C., Kirsten J., Kucaba T., Hillier L.W., Jier M., Johnston L., Langston Y., Latreille P. , Louis E.J., Macri C., Mardis E., Menezes S., Mouser L., Nhan M., Rifkin L., Riles L., St Peter H., Trevaskis E., Vaughan K., Vignati D., Wilcox L., Wohldman P., Waterston R., Wilson R., Vaudin M. Science 265:2077-2082(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[2]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[3]	"Identification and characterization of the genes for mitochondrial ribosomal proteins of Saccharomyces cerevisiae." Kitakawa M., Graack H.-R., Grohmann L., Goldschmidt-Reisin S., Herfurth E., Wittmann-Liebold B., Nishimura T., Isono K. Eur. J. Biochem. 245:449-456(1997) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 36-44. Strain: S288c / DC-5A.
[4]	"Carboxyl methylation of the phosphoprotein phosphatase 2A catalytic subunit promotes its functional association with regulatory subunits in vivo." Wu J., Tolstykh T., Lee J., Boyd K., Stock J.B., Broach J.R. EMBO J. 19:5672-5681(2000) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
[5]	"A new enrichment approach identifies genes that alter cell cycle progression in Saccharomyces cerevisiae." Bogomolnaya L.M., Pathak R., Cham R., Guo J., Surovtseva Y.V., Jaeckel L., Polymenis M. Curr. Genet. 45:350-359(2004) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
[6]	"Synergistic computational and experimental proteomics approaches for more accurate detection of active serine hydrolases in yeast." Baxter S.M., Rosenblum J.S., Knutson S., Nelson M.R., Montimurro J.S., Di Gennaro J.A., Speir J.A., Burbaum J.J., Fetrow J.S. Mol. Cell. Proteomics 3:209-225(2004) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION AS A SERINE HYDROLASE, MASS SPECTROMETRY.
[7]	"Specific interactions of PP2A and PP2A-like phosphatases with the yeast PTPA homologues, Ypa1 and Ypa2." Van Hoof C., Martens E., Longin S., Jordens J., Stevens I., Janssens V., Goris J. Biochem. J. 386:93-102(2005) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, INTERACTION WITH PPG1; PPH21; PPH22; PPH3 AND SIT4.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U10556 Genomic DNA. Translation: AAB68892.1. BK006934 Genomic DNA. Translation: DAA06769.1.
PIR	S46814.
RefSeq	NP_011942.1. NM_001179205.1.
3D structure databases
ProteinModelPortal	P38796.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-1943N.
IntAct	P38796. 4 interactions.
MINT	MINT-410794.
STRING	4932.YHR075C.
Proteomic databases
PaxDb	P38796.
PeptideAtlas	P38796.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YHR075C; YHR075C; YHR075C.
GeneID	856474.
KEGG	sce:YHR075C.
Organism-specific databases
CYGD	YHR075c.
SGD	S000001117. PPE1.
Phylogenomic databases
eggNOG	COG0596.
GeneTree	ENSGT00390000004396.
HOGENOM	HOG000212435.
KO	K13617.
OMA	GTDRLDK.
OrthoDB	EOG45QMPB.
Gene expression databases
Genevestigator	P38796.
GermOnline	YHR075C. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR000073. AB_hydrolase_1. IPR016812. PPase_methylesterase_euk. [Graphical view]
PANTHER	PTHR14189. PTHR14189. 1 hit.
Pfam	PF00561. Abhydrolase_1. 1 hit. [Graphical view]
PIRSF	PIRSF022950. PPase_methylesterase_euk. 1 hit.
ProtoNet	Search...
Other
NextBio	982146.

Entry information

Entry name

PPME1_YEAST

Accession

Primary (citable) accession number: P38796
Secondary accession number(s): D3DL25

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1995
Last sequence update:	February 1, 1995
Last modified:	May 1, 2013
This is version 108 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program