Glutamine-dependent NAD(+) synthetase - Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

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P38795 (NADE_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified May 29, 2013. Version 114. History...

Clusters with 100%, 90%, 50% identity |

Documents (4) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Glutamine-dependent NAD(+) synthetase
EC=6.3.5.1

Alternative name(s):
NAD(+) synthase [glutamine-hydrolyzing]

Gene names

Name:	QNS1
Ordered Locus Names:	YHR074W

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces ›

Protein attributes

Sequence length	714 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Catalytic activity	ATP + deamido-NAD⁺ + L-glutamine + H₂O = AMP + diphosphate + NAD⁺ + L-glutamate.
Pathway	Cofactor biosynthesis; NAD(+) biosynthesis; NAD(+) from deamido-NAD(+) (L-Gln route): step 1/1.
Miscellaneous	Present with 172 molecules/cell in log phase SD medium.
Sequence similarities	In the C-terminal section; belongs to the NAD synthetase family. Contains 1 CN hydrolase domain.

Ontologies

Keywords
Ligand	ATP-binding NAD Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	NAD biosynthetic process Inferred from direct assay PubMed 12771147 PubMed 12898714. Source: SGD
Cellular_component	cytoplasm Inferred from direct assay PubMed 12898714. Source: SGD nucleus Inferred from direct assay PubMed 12898714. Source: SGD
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW NAD+ synthase (glutamine-hydrolyzing) activity Inferred from direct assay PubMed 12771147 PubMed 12898714. Source: SGD hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, in linear amides Inferred from mutant phenotype PubMed 12771147. Source: SGD
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
itself		3	EBI-11799,EBI-11799

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 714

714

Glutamine-dependent NAD(+) synthetase

PRO_0000152248

Regions

Domain

5 – 296

292

CN hydrolase

Nucleotide binding

359 – 366

ATP By similarity

Region

329 – 714

386

Ligase

Sites

Active site

361

By similarity

Sequences

Sequence

Length

Mass (Da)

Tools

P38795 [UniParc].

Last modified February 1, 1995. Version 1.
Checksum: ED8C723550F9E8A9
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FASTA

714

80,686

        10         20         30         40         50         60 
MSHLITLATC NLNQWALDFE GNRDRILQSI KIAKERGARL RVGPELEITG YGCLDHFLEN 

        70         80         90        100        110        120 
DVCLHSWEMY AQIIKNKETH GLILDIGMPV LHKNVRYNCR LLSLDGEILF IRPKIWLAND 

       130        140        150        160        170        180 
GNYREMRFFT PWMKPGVVED FILPPEIQKV TGQRLVPFGD AVINSLDTCI GTETCEELFT 

       190        200        210        220        230        240 
PQSPHIAMSL DGVEIMTNSS GSHHELRKLN KRLDLILNAT KRCGGVYLYA NQRGCDGDRL 

       250        260        270        280        290        300 
YYDGCALIAI NGTIVAQGSQ FSLDDVEVVT ATVDLEEVRS YRAAVMSRGL QASLAEIKFK 

       310        320        330        340        350        360 
RIDIPVELAL MTSRFDPTVC PTKVREPFYH SPEEEIALGP ACWMWDYLRR CNGTGFFLPL 

       370        380        390        400        410        420 
SGGIDSCATA MIVHSMCRLV TDAAQNGNEQ VIKDVRKITR SGDDWIPDSP QDLASKIFHS 

       430        440        450        460        470        480 
CFMGTENSSK ETRNRAKDLS NAIGSYHVDL KMDSLVSSVV SLFEVATGKK PIYKIFGGSQ 

       490        500        510        520        530        540 
IENLALQNIQ ARLRMVLSYL FAQLLPWVRG IPNSGGLLVL GSANVDECLR GYLTKYDCSS 

       550        560        570        580        590        600 
ADINPIGGIS KTDLKRFIAY ASKQYNMPIL NDFLNATPTA ELEPMTKDYV QSDEIDMGMT 

       610        620        630        640        650        660 
YEELGVFGYL RKVEKCGPYS MFLKLLHQWS PKLTPRQISE KVKRFFFFYA INRHKQTVLT 

       670        680        690        700        710 
PSYHAEQYSP EDNRFDLRPF LINPRFPWAS RKIDEVVEQC EAHKGSTLDI MSID

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Complete nucleotide sequence of Saccharomyces cerevisiae chromosome VIII." Johnston M., Andrews S., Brinkman R., Cooper J., Ding H., Dover J., Du Z., Favello A., Fulton L., Gattung S., Geisel C., Kirsten J., Kucaba T., Hillier L.W., Jier M., Johnston L., Langston Y., Latreille P. Vaudin M. Science 265:2077-2082(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[2]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[3]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U10556 Genomic DNA. Translation: AAB68889.1. BK006934 Genomic DNA. Translation: DAA06768.1.
PIR	S46811.
RefSeq	NP_011941.1. NM_001179204.1.
3D structure databases
ProteinModelPortal	P38795.
SMR	P38795. Positions 4-663.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-5592N.
IntAct	P38795. 2 interactions.
MINT	MINT-479617.
STRING	4932.YHR074W.
Proteomic databases
PaxDb	P38795.
PeptideAtlas	P38795.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YHR074W; YHR074W; YHR074W.
GeneID	856473.
KEGG	sce:YHR074W.
Organism-specific databases
CYGD	YHR074w.
SGD	S000001116. QNS1.
Phylogenomic databases
eggNOG	COG0388.
GeneTree	ENSGT00390000010152.
HOGENOM	HOG000160137.
KO	K01950.
OMA	DFALSCH.
OrthoDB	EOG4XKZG2.
Enzyme and pathway databases
BioCyc	YEAST:MONOMER3O-845.
UniPathway	UPA00253; UER00334.
Gene expression databases
Genevestigator	P38795.
GermOnline	YHR074W. Saccharomyces cerevisiae.
Family and domain databases
Gene3D	3.40.50.620. 1 hit. 3.60.110.10. 1 hit.
InterPro	IPR003010. C-N_Hydrolase. IPR014445. Gln-dep_NAD_synthase. IPR022310. NAD/GMP_synthase. IPR003694. NAD_synthase. IPR014729. Rossmann-like_a/b/a_fold. [Graphical view]
Pfam	PF00795. CN_hydrolase. 1 hit. PF02540. NAD_synthase. 1 hit. [Graphical view]
PIRSF	PIRSF006630. NADS_GAT. 1 hit.
SUPFAM	SSF56317. Ntlse/CNhydtse. 1 hit.
TIGRFAMs	TIGR00552. nadE. 1 hit.
PROSITE	PS50263. CN_HYDROLASE. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	982143.

Entry information

Entry name

NADE_YEAST

Accession

Primary (citable) accession number: P38795
Secondary accession number(s): D3DL24

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1995
Last sequence update:	February 1, 1995
Last modified:	May 29, 2013
This is version 114 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program

Relevant documents

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

Yeast chromosome VIII

Yeast (Saccharomyces cerevisiae) chromosome VIII: entries and gene names

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Binary interactions

With

Entry

#Exp.

IntAct

Notes

Sequence annotation (Features)

Molecule processing

Regions

Sites

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents