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Protein

Glutamine-dependent NAD(+) synthetase

Gene

QNS1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

ATP + deamido-NAD+ + L-glutamine + H2O = AMP + diphosphate + NAD+ + L-glutamate.

Pathwayi: NAD(+) biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes NAD(+) from deamido-NAD(+) (L-Gln route).
Proteins known to be involved in this subpathway in this organism are:
  1. Glutamine-dependent NAD(+) synthetase (QNS1)
This subpathway is part of the pathway NAD(+) biosynthesis, which is itself part of Cofactor biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes NAD(+) from deamido-NAD(+) (L-Gln route), the pathway NAD(+) biosynthesis and in Cofactor biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei361PROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi359 – 366ATPBy similarity8

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • glutaminase activity Source: SGD
  • NAD+ synthase (glutamine-hydrolyzing) activity Source: SGD

GO - Biological processi

  • NAD biosynthetic process Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Ligandi

ATP-binding, NAD, Nucleotide-binding

Enzyme and pathway databases

BioCyciYEAST:MONOMER3O-845.
ReactomeiR-SCE-196807. Nicotinate metabolism.
UniPathwayiUPA00253; UER00334.

Names & Taxonomyi

Protein namesi
Recommended name:
Glutamine-dependent NAD(+) synthetase (EC:6.3.5.1)
Alternative name(s):
NAD(+) synthase [glutamine-hydrolyzing]
Gene namesi
Name:QNS1
Ordered Locus Names:YHR074W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome VIII

Organism-specific databases

EuPathDBiFungiDB:YHR074W.
SGDiS000001116. QNS1.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: SGD
  • nucleus Source: SGD
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001522481 – 714Glutamine-dependent NAD(+) synthetaseAdd BLAST714

Proteomic databases

MaxQBiP38795.
PRIDEiP38795.

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
itself3EBI-11799,EBI-11799

Protein-protein interaction databases

BioGridi36508. 28 interactors.
DIPiDIP-5592N.
IntActiP38795. 1 interactor.
MINTiMINT-479617.

Structurei

3D structure databases

ProteinModelPortaliP38795.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini5 – 296CN hydrolasePROSITE-ProRule annotationAdd BLAST292

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni329 – 714LigaseAdd BLAST386

Sequence similaritiesi

In the C-terminal section; belongs to the NAD synthetase family.Curated
Contains 1 CN hydrolase domain.PROSITE-ProRule annotation

Phylogenomic databases

GeneTreeiENSGT00390000010152.
HOGENOMiHOG000160137.
InParanoidiP38795.
KOiK01950.
OMAiCATAIIV.
OrthoDBiEOG092C116R.

Family and domain databases

CDDicd00553. NAD_synthase. 1 hit.
Gene3Di3.40.50.620. 1 hit.
3.60.110.10. 1 hit.
InterProiIPR003010. C-N_Hydrolase.
IPR014445. Gln-dep_NAD_synthase.
IPR022310. NAD/GMP_synthase.
IPR003694. NAD_synthase.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PfamiPF00795. CN_hydrolase. 1 hit.
PF02540. NAD_synthase. 1 hit.
[Graphical view]
PIRSFiPIRSF006630. NADS_GAT. 1 hit.
SUPFAMiSSF56317. SSF56317. 1 hit.
TIGRFAMsiTIGR00552. nadE. 1 hit.
PROSITEiPS50263. CN_HYDROLASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P38795-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSHLITLATC NLNQWALDFE GNRDRILQSI KIAKERGARL RVGPELEITG
60 70 80 90 100
YGCLDHFLEN DVCLHSWEMY AQIIKNKETH GLILDIGMPV LHKNVRYNCR
110 120 130 140 150
LLSLDGEILF IRPKIWLAND GNYREMRFFT PWMKPGVVED FILPPEIQKV
160 170 180 190 200
TGQRLVPFGD AVINSLDTCI GTETCEELFT PQSPHIAMSL DGVEIMTNSS
210 220 230 240 250
GSHHELRKLN KRLDLILNAT KRCGGVYLYA NQRGCDGDRL YYDGCALIAI
260 270 280 290 300
NGTIVAQGSQ FSLDDVEVVT ATVDLEEVRS YRAAVMSRGL QASLAEIKFK
310 320 330 340 350
RIDIPVELAL MTSRFDPTVC PTKVREPFYH SPEEEIALGP ACWMWDYLRR
360 370 380 390 400
CNGTGFFLPL SGGIDSCATA MIVHSMCRLV TDAAQNGNEQ VIKDVRKITR
410 420 430 440 450
SGDDWIPDSP QDLASKIFHS CFMGTENSSK ETRNRAKDLS NAIGSYHVDL
460 470 480 490 500
KMDSLVSSVV SLFEVATGKK PIYKIFGGSQ IENLALQNIQ ARLRMVLSYL
510 520 530 540 550
FAQLLPWVRG IPNSGGLLVL GSANVDECLR GYLTKYDCSS ADINPIGGIS
560 570 580 590 600
KTDLKRFIAY ASKQYNMPIL NDFLNATPTA ELEPMTKDYV QSDEIDMGMT
610 620 630 640 650
YEELGVFGYL RKVEKCGPYS MFLKLLHQWS PKLTPRQISE KVKRFFFFYA
660 670 680 690 700
INRHKQTVLT PSYHAEQYSP EDNRFDLRPF LINPRFPWAS RKIDEVVEQC
710
EAHKGSTLDI MSID
Length:714
Mass (Da):80,686
Last modified:February 1, 1995 - v1
Checksum:iED8C723550F9E8A9
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U10556 Genomic DNA. Translation: AAB68889.1.
BK006934 Genomic DNA. Translation: DAA06768.1.
PIRiS46811.
RefSeqiNP_011941.1. NM_001179204.1.

Genome annotation databases

EnsemblFungiiYHR074W; YHR074W; YHR074W.
GeneIDi856473.
KEGGisce:YHR074W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U10556 Genomic DNA. Translation: AAB68889.1.
BK006934 Genomic DNA. Translation: DAA06768.1.
PIRiS46811.
RefSeqiNP_011941.1. NM_001179204.1.

3D structure databases

ProteinModelPortaliP38795.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi36508. 28 interactors.
DIPiDIP-5592N.
IntActiP38795. 1 interactor.
MINTiMINT-479617.

Proteomic databases

MaxQBiP38795.
PRIDEiP38795.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYHR074W; YHR074W; YHR074W.
GeneIDi856473.
KEGGisce:YHR074W.

Organism-specific databases

EuPathDBiFungiDB:YHR074W.
SGDiS000001116. QNS1.

Phylogenomic databases

GeneTreeiENSGT00390000010152.
HOGENOMiHOG000160137.
InParanoidiP38795.
KOiK01950.
OMAiCATAIIV.
OrthoDBiEOG092C116R.

Enzyme and pathway databases

UniPathwayiUPA00253; UER00334.
BioCyciYEAST:MONOMER3O-845.
ReactomeiR-SCE-196807. Nicotinate metabolism.

Miscellaneous databases

PROiP38795.

Family and domain databases

CDDicd00553. NAD_synthase. 1 hit.
Gene3Di3.40.50.620. 1 hit.
3.60.110.10. 1 hit.
InterProiIPR003010. C-N_Hydrolase.
IPR014445. Gln-dep_NAD_synthase.
IPR022310. NAD/GMP_synthase.
IPR003694. NAD_synthase.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PfamiPF00795. CN_hydrolase. 1 hit.
PF02540. NAD_synthase. 1 hit.
[Graphical view]
PIRSFiPIRSF006630. NADS_GAT. 1 hit.
SUPFAMiSSF56317. SSF56317. 1 hit.
TIGRFAMsiTIGR00552. nadE. 1 hit.
PROSITEiPS50263. CN_HYDROLASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiNADE_YEAST
AccessioniPrimary (citable) accession number: P38795
Secondary accession number(s): D3DL24
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: February 1, 1995
Last modified: November 2, 2016
This is version 146 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 172 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome VIII
    Yeast (Saccharomyces cerevisiae) chromosome VIII: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.