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Protein

Glutamine-dependent NAD(+) synthetase

Gene

QNS1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Miscellaneous

Present with 172 molecules/cell in log phase SD medium.1 Publication

Catalytic activityi

ATP + deamido-NAD+ + L-glutamine + H2O = AMP + diphosphate + NAD+ + L-glutamate.

Pathwayi: NAD(+) biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes NAD(+) from deamido-NAD(+) (L-Gln route).
Proteins known to be involved in this subpathway in this organism are:
  1. Glutamine-dependent NAD(+) synthetase (QNS1)
This subpathway is part of the pathway NAD(+) biosynthesis, which is itself part of Cofactor biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes NAD(+) from deamido-NAD(+) (L-Gln route), the pathway NAD(+) biosynthesis and in Cofactor biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei45Proton acceptor; for glutaminase activityBy similarity1
Active sitei114For glutaminase activityBy similarity1
Active sitei175Nucleophile; for glutaminase activityBy similarity1
Active sitei361By similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi359 – 366ATPBy similarity8

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • glutaminase activity Source: SGD
  • NAD+ synthase (glutamine-hydrolyzing) activity Source: SGD

GO - Biological processi

  • NAD biosynthetic process Source: SGD

Keywordsi

Molecular functionLigase
LigandATP-binding, NAD, Nucleotide-binding

Enzyme and pathway databases

BioCyciYEAST:MONOMER3O-845
ReactomeiR-SCE-196807 Nicotinate metabolism
UniPathwayiUPA00253; UER00334

Names & Taxonomyi

Protein namesi
Recommended name:
Glutamine-dependent NAD(+) synthetase (EC:6.3.5.1)
Alternative name(s):
NAD(+) synthase [glutamine-hydrolyzing]
Gene namesi
Name:QNS1
Ordered Locus Names:YHR074W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome VIII

Organism-specific databases

EuPathDBiFungiDB:YHR074W
SGDiS000001116 QNS1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001522481 – 714Glutamine-dependent NAD(+) synthetaseAdd BLAST714

Proteomic databases

MaxQBiP38795
PaxDbiP38795
PRIDEiP38795

Interactioni

Protein-protein interaction databases

BioGridi36508, 171 interactors
DIPiDIP-5592N
IntActiP38795, 3 interactors
MINTiP38795
STRINGi4932.YHR074W

Structurei

3D structure databases

ProteinModelPortaliP38795
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini5 – 275CN hydrolasePROSITE-ProRule annotationAdd BLAST271

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni329 – 714LigaseAdd BLAST386

Sequence similaritiesi

In the C-terminal section; belongs to the NAD synthetase family.Curated

Phylogenomic databases

GeneTreeiENSGT00390000010152
HOGENOMiHOG000160137
InParanoidiP38795
KOiK01950
OMAiARNRHKM
OrthoDBiEOG092C116R

Family and domain databases

CDDicd00553 NAD_synthase, 1 hit
Gene3Di3.40.50.620, 1 hit
3.60.110.10, 1 hit
HAMAPiMF_02090 NadE_glutamine_dep, 1 hit
InterProiView protein in InterPro
IPR003010 C-N_Hydrolase
IPR036526 C-N_Hydrolase_sf
IPR014445 Gln-dep_NAD_synthase
IPR022310 NAD/GMP_synthase
IPR003694 NAD_synthase
IPR014729 Rossmann-like_a/b/a_fold
PANTHERiPTHR23090 PTHR23090, 1 hit
PfamiView protein in Pfam
PF00795 CN_hydrolase, 1 hit
PF02540 NAD_synthase, 1 hit
PIRSFiPIRSF006630 NADS_GAT, 1 hit
SUPFAMiSSF56317 SSF56317, 1 hit
TIGRFAMsiTIGR00552 nadE, 1 hit
PROSITEiView protein in PROSITE
PS50263 CN_HYDROLASE, 1 hit

Sequencei

Sequence statusi: Complete.

P38795-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSHLITLATC NLNQWALDFE GNRDRILQSI KIAKERGARL RVGPELEITG
60 70 80 90 100
YGCLDHFLEN DVCLHSWEMY AQIIKNKETH GLILDIGMPV LHKNVRYNCR
110 120 130 140 150
LLSLDGEILF IRPKIWLAND GNYREMRFFT PWMKPGVVED FILPPEIQKV
160 170 180 190 200
TGQRLVPFGD AVINSLDTCI GTETCEELFT PQSPHIAMSL DGVEIMTNSS
210 220 230 240 250
GSHHELRKLN KRLDLILNAT KRCGGVYLYA NQRGCDGDRL YYDGCALIAI
260 270 280 290 300
NGTIVAQGSQ FSLDDVEVVT ATVDLEEVRS YRAAVMSRGL QASLAEIKFK
310 320 330 340 350
RIDIPVELAL MTSRFDPTVC PTKVREPFYH SPEEEIALGP ACWMWDYLRR
360 370 380 390 400
CNGTGFFLPL SGGIDSCATA MIVHSMCRLV TDAAQNGNEQ VIKDVRKITR
410 420 430 440 450
SGDDWIPDSP QDLASKIFHS CFMGTENSSK ETRNRAKDLS NAIGSYHVDL
460 470 480 490 500
KMDSLVSSVV SLFEVATGKK PIYKIFGGSQ IENLALQNIQ ARLRMVLSYL
510 520 530 540 550
FAQLLPWVRG IPNSGGLLVL GSANVDECLR GYLTKYDCSS ADINPIGGIS
560 570 580 590 600
KTDLKRFIAY ASKQYNMPIL NDFLNATPTA ELEPMTKDYV QSDEIDMGMT
610 620 630 640 650
YEELGVFGYL RKVEKCGPYS MFLKLLHQWS PKLTPRQISE KVKRFFFFYA
660 670 680 690 700
INRHKQTVLT PSYHAEQYSP EDNRFDLRPF LINPRFPWAS RKIDEVVEQC
710
EAHKGSTLDI MSID
Length:714
Mass (Da):80,686
Last modified:February 1, 1995 - v1
Checksum:iED8C723550F9E8A9
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U10556 Genomic DNA Translation: AAB68889.1
BK006934 Genomic DNA Translation: DAA06768.1
PIRiS46811
RefSeqiNP_011941.1, NM_001179204.1

Genome annotation databases

EnsemblFungiiYHR074W; YHR074W; YHR074W
GeneIDi856473
KEGGisce:YHR074W

Similar proteinsi

Entry informationi

Entry nameiNADE_YEAST
AccessioniPrimary (citable) accession number: P38795
Secondary accession number(s): D3DL24
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: February 1, 1995
Last modified: June 20, 2018
This is version 159 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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