Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

P38793 (TRM5_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 113. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
tRNA (guanine(37)-N1)-methyltransferase

EC=2.1.1.228
Alternative name(s):
M1G-methyltransferase
tRNA [GM37] methyltransferase
tRNA methyltransferase 5
Gene names
Name:TRM5
Ordered Locus Names:YHR070W
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length499 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Specifically methylates the N1 position of guanosine-37 in various cytoplasmic and mitochondrial tRNAs. Methylation is not dependent on the nature of the nucleoside 5' of the target nucleoside. This is the first step in the biosynthesis of wybutosine (yW), a modified base adjacent to the anticodon of tRNAs and required for accurate decoding. Postspliced cytoplasmic tRNAs are imported into the nucleus, where this first step seems to take place, after which they are reexported to the cytoplasm, where the yW sythesis is completed by cytoplasmic enzymes. Ref.3 Ref.6 Ref.7 Ref.8

Catalytic activity

S-adenosyl-L-methionine + guanine37 in tRNA = S-adenosyl-L-homocysteine + N(1)-methylguanine37 in tRNA. HAMAP-Rule MF_03152

Subunit structure

Monomer By similarity. HAMAP-Rule MF_03152

Subcellular location

Mitochondrion matrix. Nucleus. Cytoplasm. Note: Predominantly in the mitochondria and in the nucleus. Ref.4 Ref.6 Ref.8

Miscellaneous

Present with 4120 molecules/cell in log phase SD medium.

It is unsure how the mitochondrial and cytoplasmic forms of this protein are produced. The cytoplasmic form may be produced by alternative initiation at a downstream in-frame AUG codon at position 34, lacking a mitochondrial transit peptide.

Sequence similarities

Belongs to the class I-like SAM-binding methyltransferase superfamily. TRM5/TYW2 family.

Binary interactions

With

Entry

#Exp.

IntAct

Notes

itself1EBI-24581,EBI-24581

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Transit peptide1 – 4444Mitochondrion Potential
Chain45 – 499455tRNA (guanine(37)-N1)-methyltransferase HAMAP-Rule MF_03152
PRO_0000202899

Regions

Region307 – 3082S-adenosyl-L-methionine binding By similarity
Region335 – 3362S-adenosyl-L-methionine binding By similarity

Sites

Binding site2681S-adenosyl-L-methionine By similarity
Binding site3991S-adenosyl-L-methionine By similarity

Experimental info

Mutagenesis1 – 3333Missing: Abolishes mitochondrial localization and activity, but not cytoplasmic activity of the enzyme. Ref.6

Sequences

Sequence LengthMass (Da)Tools
P38793 [UniParc].

Last modified February 1, 1995. Version 1.
Checksum: 4ADFE01440FF2188

FASTA49956,514
        10         20         30         40         50         60 
MKIALPVFQK FNRLISSCKM SGVFPYNPPV NRQMRELDRS FFITKIPMCA VKFPEPKNIS 

        70         80         90        100        110        120 
VFSKNFKNCI LRVPRIPHVV KLNSSKPKDE LTSVQNKKLK TADGNNTPVT KGVLLHESIH 

       130        140        150        160        170        180 
SVEDAYGKLP EDALAFLKEN SAEIVPHEYV LDYDFWKAEE ILRAVLPEQF LEEVPTGFTI 

       190        200        210        220        230        240 
TGHIAHLNLR TEFKPFDSLI GQVILDKNNK IECVVDKVSS IATQFRTFPM KVIAGKSDSL 

       250        260        270        280        290        300 
VVEQKESNCT FKFDFSKVYW NSRLHTEHER LVKQYFQPGQ VVCDVFAGVG PFAVPAGKKD 

       310        320        330        340        350        360 
VIVLANDLNP ESYKYLKENI ALNKVAKTVK SFNMDGADFI RQSPQLLQQW IQDEEGGKIT 

       370        380        390        400        410        420 
IPLPLKKRHR SQQHNDQQPP QPRTKELIIP SHISHYVMNL PDSAISFLGN FRGIFAAHTK 

       430        440        450        460        470        480 
GATDTIQMPW VHVHCFEKYP PGDQVTEDEL HARVHARIIA ALKVTADDLP LNAVSLHLVR 

       490 
KVAPTKPMYC ASFQLPANV 

« Hide

References

« Hide 'large scale' references
[1]"Complete nucleotide sequence of Saccharomyces cerevisiae chromosome VIII."
Johnston M., Andrews S., Brinkman R., Cooper J., Ding H., Dover J., Du Z., Favello A., Fulton L., Gattung S., Geisel C., Kirsten J., Kucaba T., Hillier L.W., Jier M., Johnston L., Langston Y., Latreille P. expand/collapse author list , Louis E.J., Macri C., Mardis E., Menezes S., Mouser L., Nhan M., Rifkin L., Riles L., St Peter H., Trevaskis E., Vaughan K., Vignati D., Wilcox L., Wohldman P., Waterston R., Wilson R., Vaudin M.
Science 265:2077-2082(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[2]Saccharomyces Genome Database
Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[3]"A primordial tRNA modification required for the evolution of life?"
Bjork G.R., Jacobsson K., Nilsson K., Johansson M.J., Bystroem A.S., Persson O.P.
EMBO J. 20:231-239(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[4]"Global analysis of protein localization in budding yeast."
Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K.
Nature 425:686-691(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[5]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[6]"Yeast mitochondrial initiator tRNA is methylated at guanosine 37 by the Trm5-encoded tRNA (guanine-N1-)-methyltransferase."
Lee C., Kramer G., Graham D.E., Appling D.R.
J. Biol. Chem. 282:27744-27753(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, SUBCELLULAR LOCATION, MUTAGENESIS OF 1-MET--GLN-33.
[7]"The use of Saccharomyces cerevisiae proteomic libraries to identify RNA-modifying proteins."
Jackman J.E., Grayhack E.J., Phizicky E.M.
Methods Mol. Biol. 488:383-393(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[8]"Retrograde nuclear import of tRNA precursors is required for modified base biogenesis in yeast."
Ohira T., Suzuki T.
Proc. Natl. Acad. Sci. U.S.A. 108:10502-10507(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, SUBCELLULAR LOCATION.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U00061 Genomic DNA. Translation: AAB68376.1.
BK006934 Genomic DNA. Translation: DAA06763.1.
PIRS46697.
RefSeqNP_011937.1. NM_001179200.1.

3D structure databases

ProteinModelPortalP38793.
SMRP38793. Positions 157-359.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid36502. 12 interactions.
DIPDIP-2950N.
IntActP38793. 2 interactions.
MINTMINT-582793.
STRING4932.YHR070W.

Proteomic databases

PaxDbP38793.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYHR070W; YHR070W; YHR070W.
GeneID856467.
KEGGsce:YHR070W.

Organism-specific databases

CYGDYHR070w.
SGDS000001112. TRM5.

Phylogenomic databases

eggNOGCOG2520.
GeneTreeENSGT00390000008208.
HOGENOMHOG000195270.
KOK15429.
OMAPTKPMFC.
OrthoDBEOG75TMPT.

Enzyme and pathway databases

BioCycMetaCyc:G3O-31120-MONOMER.
YEAST:G3O-31120-MONOMER.

Gene expression databases

GenevestigatorP38793.

Family and domain databases

HAMAPMF_03152. TRM5.
InterProIPR025792. tRNA_Gua_MeTrfase_euk.
IPR003402. tRNA_Trfase_Trm5/Tyw2.
[Graphical view]
PfamPF02475. Met_10. 1 hit.
[Graphical view]
PROSITEPS51684. SAM_MT_TRM5_TYW2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio982126.
PROP38793.

Entry information

Entry nameTRM5_YEAST
AccessionPrimary (citable) accession number: P38793
Secondary accession number(s): D3DL19
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: February 1, 1995
Last modified: April 16, 2014
This is version 113 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome VIII

Yeast (Saccharomyces cerevisiae) chromosome VIII: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families