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Protein

tRNA (guanine(37)-N1)-methyltransferase

Gene

TRM5

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Specifically methylates the N1 position of guanosine-37 in various cytoplasmic and mitochondrial tRNAs. Methylation is not dependent on the nature of the nucleoside 5' of the target nucleoside. This is the first step in the biosynthesis of wybutosine (yW), a modified base adjacent to the anticodon of tRNAs and required for accurate decoding. Postspliced cytoplasmic tRNAs are imported into the nucleus, where this first step seems to take place, after which they are reexported to the cytoplasm, where the yW sythesis is completed by cytoplasmic enzymes.UniRule annotation4 Publications

Miscellaneous

Present with 4120 molecules/cell in log phase SD medium.1 Publication
It is unsure how the mitochondrial and cytoplasmic forms of this protein are produced. The cytoplasmic form may be produced by alternative initiation at a downstream in-frame AUG codon at position 34, lacking a mitochondrial transit peptide.

Catalytic activityi

S-adenosyl-L-methionine + guanine(37) in tRNA = S-adenosyl-L-homocysteine + N1-methylguanine(37) in tRNA.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei268S-adenosyl-L-methionineUniRule annotation1
Binding sitei399S-adenosyl-L-methionineUniRule annotation1

GO - Molecular functioni

GO - Biological processi

  • mitochondrial tRNA methylation Source: SGD
  • tRNA modification Source: Reactome
  • tRNA N1-guanine methylation Source: SGD

Keywordsi

Molecular functionMethyltransferase, Transferase
Biological processtRNA processing
LigandS-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciMetaCyc:G3O-31120-MONOMER.
YEAST:G3O-31120-MONOMER.
BRENDAi2.1.1.228. 984.
ReactomeiR-SCE-6782861. Synthesis of wybutosine at G37 of tRNA(Phe).

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA (guanine(37)-N1)-methyltransferaseUniRule annotation (EC:2.1.1.228UniRule annotation)
Alternative name(s):
M1G-methyltransferaseUniRule annotation
tRNA [GM37] methyltransferaseUniRule annotation
tRNA methyltransferase 5UniRule annotation
Gene namesi
Name:TRM5UniRule annotation
Ordered Locus Names:YHR070W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome VIII

Organism-specific databases

EuPathDBiFungiDB:YHR070W.
SGDiS000001112. TRM5.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Mitochondrion, Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi1 – 33Missing : Abolishes mitochondrial localization and activity, but not cytoplasmic activity of the enzyme. 1 PublicationAdd BLAST33

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 44MitochondrionUniRule annotationAdd BLAST44
ChainiPRO_000020289945 – 499tRNA (guanine(37)-N1)-methyltransferaseAdd BLAST455

Proteomic databases

MaxQBiP38793.
PRIDEiP38793.

Interactioni

Subunit structurei

Monomer.UniRule annotation

Binary interactionsi

Show more details

Protein-protein interaction databases

BioGridi36502. 13 interactors.
DIPiDIP-2950N.
IntActiP38793. 8 interactors.
MINTiMINT-582793.
STRINGi4932.YHR070W.

Structurei

3D structure databases

ProteinModelPortaliP38793.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni307 – 308S-adenosyl-L-methionine bindingUniRule annotation2
Regioni335 – 336S-adenosyl-L-methionine bindingUniRule annotation2

Sequence similaritiesi

Keywords - Domaini

Transit peptide

Phylogenomic databases

GeneTreeiENSGT00390000008208.
HOGENOMiHOG000195270.
InParanoidiP38793.
KOiK15429.
OMAiKHLIGQV.
OrthoDBiEOG092C2JLG.

Family and domain databases

HAMAPiMF_03152. TRM5. 1 hit.
InterProiView protein in InterPro
IPR030382. MeTrfase_TRM5/TYW2.
IPR029063. SAM-dependent_MTases.
IPR025792. tRNA_Gua_MeTrfase_euk.
PfamiView protein in Pfam
PF02475. Met_10. 1 hit.
SUPFAMiSSF53335. SSF53335. 2 hits.
PROSITEiView protein in PROSITE
PS51684. SAM_MT_TRM5_TYW2. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P38793-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKIALPVFQK FNRLISSCKM SGVFPYNPPV NRQMRELDRS FFITKIPMCA
60 70 80 90 100
VKFPEPKNIS VFSKNFKNCI LRVPRIPHVV KLNSSKPKDE LTSVQNKKLK
110 120 130 140 150
TADGNNTPVT KGVLLHESIH SVEDAYGKLP EDALAFLKEN SAEIVPHEYV
160 170 180 190 200
LDYDFWKAEE ILRAVLPEQF LEEVPTGFTI TGHIAHLNLR TEFKPFDSLI
210 220 230 240 250
GQVILDKNNK IECVVDKVSS IATQFRTFPM KVIAGKSDSL VVEQKESNCT
260 270 280 290 300
FKFDFSKVYW NSRLHTEHER LVKQYFQPGQ VVCDVFAGVG PFAVPAGKKD
310 320 330 340 350
VIVLANDLNP ESYKYLKENI ALNKVAKTVK SFNMDGADFI RQSPQLLQQW
360 370 380 390 400
IQDEEGGKIT IPLPLKKRHR SQQHNDQQPP QPRTKELIIP SHISHYVMNL
410 420 430 440 450
PDSAISFLGN FRGIFAAHTK GATDTIQMPW VHVHCFEKYP PGDQVTEDEL
460 470 480 490
HARVHARIIA ALKVTADDLP LNAVSLHLVR KVAPTKPMYC ASFQLPANV
Length:499
Mass (Da):56,514
Last modified:February 1, 1995 - v1
Checksum:i4ADFE01440FF2188
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00061 Genomic DNA. Translation: AAB68376.1.
BK006934 Genomic DNA. Translation: DAA06763.1.
PIRiS46697.
RefSeqiNP_011937.1. NM_001179200.1.

Genome annotation databases

EnsemblFungiiYHR070W; YHR070W; YHR070W.
GeneIDi856467.
KEGGisce:YHR070W.

Similar proteinsi

Entry informationi

Entry nameiTRM5_YEAST
AccessioniPrimary (citable) accession number: P38793
Secondary accession number(s): D3DL19
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: February 1, 1995
Last modified: September 27, 2017
This is version 141 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome VIII
    Yeast (Saccharomyces cerevisiae) chromosome VIII: entries and gene names