Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

tRNA (guanine(37)-N1)-methyltransferase

Gene

TRM5

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Specifically methylates the N1 position of guanosine-37 in various cytoplasmic and mitochondrial tRNAs. Methylation is not dependent on the nature of the nucleoside 5' of the target nucleoside. This is the first step in the biosynthesis of wybutosine (yW), a modified base adjacent to the anticodon of tRNAs and required for accurate decoding. Postspliced cytoplasmic tRNAs are imported into the nucleus, where this first step seems to take place, after which they are reexported to the cytoplasm, where the yW sythesis is completed by cytoplasmic enzymes.UniRule annotation4 Publications

Catalytic activityi

S-adenosyl-L-methionine + guanine(37) in tRNA = S-adenosyl-L-homocysteine + N(1)-methylguanine(37) in tRNA.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei268 – 2681S-adenosyl-L-methionineUniRule annotation
Binding sitei399 – 3991S-adenosyl-L-methionineUniRule annotation

GO - Molecular functioni

  • tRNA (guanine(37)-N(1))-methyltransferase activity Source: UniProtKB-EC
  • tRNA (guanine) methyltransferase activity Source: SGD

GO - Biological processi

  • tRNA methylation Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

tRNA processing

Keywords - Ligandi

S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciMetaCyc:G3O-31120-MONOMER.
YEAST:G3O-31120-MONOMER.
BRENDAi2.1.1.228. 984.

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA (guanine(37)-N1)-methyltransferaseUniRule annotation (EC:2.1.1.228UniRule annotation)
Alternative name(s):
M1G-methyltransferaseUniRule annotation
tRNA [GM37] methyltransferaseUniRule annotation
tRNA methyltransferase 5UniRule annotation
Gene namesi
Name:TRM5UniRule annotation
Ordered Locus Names:YHR070W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311 Componenti: Chromosome VIII

Organism-specific databases

CYGDiYHR070w.
EuPathDBiFungiDB:YHR070W.
SGDiS000001112. TRM5.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: SGD
  • mitochondrial matrix Source: SGD
  • nucleus Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Mitochondrion, Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi1 – 3333Missing : Abolishes mitochondrial localization and activity, but not cytoplasmic activity of the enzyme. 1 PublicationAdd
BLAST

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 4444MitochondrionUniRule annotationAdd
BLAST
Chaini45 – 499455tRNA (guanine(37)-N1)-methyltransferasePRO_0000202899Add
BLAST

Proteomic databases

MaxQBiP38793.
PaxDbiP38793.

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

BioGridi36502. 12 interactions.
DIPiDIP-2950N.
IntActiP38793. 2 interactions.
MINTiMINT-582793.

Structurei

3D structure databases

ProteinModelPortaliP38793.
SMRiP38793. Positions 157-339.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni307 – 3082S-adenosyl-L-methionine bindingUniRule annotation
Regioni335 – 3362S-adenosyl-L-methionine bindingUniRule annotation

Sequence similaritiesi

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiCOG2520.
GeneTreeiENSGT00390000008208.
HOGENOMiHOG000195270.
InParanoidiP38793.
KOiK15429.
OMAiHEYVLDY.
OrthoDBiEOG75TMPT.

Family and domain databases

Gene3Di3.40.50.150. 2 hits.
HAMAPiMF_03152. TRM5.
InterProiIPR030382. MeTrfase_TRM5/TYW2.
IPR029063. SAM-dependent_MTases.
IPR025792. tRNA_Gua_MeTrfase_euk.
[Graphical view]
PfamiPF02475. Met_10. 1 hit.
[Graphical view]
SUPFAMiSSF53335. SSF53335. 2 hits.
PROSITEiPS51684. SAM_MT_TRM5_TYW2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P38793-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKIALPVFQK FNRLISSCKM SGVFPYNPPV NRQMRELDRS FFITKIPMCA
60 70 80 90 100
VKFPEPKNIS VFSKNFKNCI LRVPRIPHVV KLNSSKPKDE LTSVQNKKLK
110 120 130 140 150
TADGNNTPVT KGVLLHESIH SVEDAYGKLP EDALAFLKEN SAEIVPHEYV
160 170 180 190 200
LDYDFWKAEE ILRAVLPEQF LEEVPTGFTI TGHIAHLNLR TEFKPFDSLI
210 220 230 240 250
GQVILDKNNK IECVVDKVSS IATQFRTFPM KVIAGKSDSL VVEQKESNCT
260 270 280 290 300
FKFDFSKVYW NSRLHTEHER LVKQYFQPGQ VVCDVFAGVG PFAVPAGKKD
310 320 330 340 350
VIVLANDLNP ESYKYLKENI ALNKVAKTVK SFNMDGADFI RQSPQLLQQW
360 370 380 390 400
IQDEEGGKIT IPLPLKKRHR SQQHNDQQPP QPRTKELIIP SHISHYVMNL
410 420 430 440 450
PDSAISFLGN FRGIFAAHTK GATDTIQMPW VHVHCFEKYP PGDQVTEDEL
460 470 480 490
HARVHARIIA ALKVTADDLP LNAVSLHLVR KVAPTKPMYC ASFQLPANV
Length:499
Mass (Da):56,514
Last modified:February 1, 1995 - v1
Checksum:i4ADFE01440FF2188
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00061 Genomic DNA. Translation: AAB68376.1.
BK006934 Genomic DNA. Translation: DAA06763.1.
PIRiS46697.
RefSeqiNP_011937.1. NM_001179200.1.

Genome annotation databases

EnsemblFungiiYHR070W; YHR070W; YHR070W.
GeneIDi856467.
KEGGisce:YHR070W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00061 Genomic DNA. Translation: AAB68376.1.
BK006934 Genomic DNA. Translation: DAA06763.1.
PIRiS46697.
RefSeqiNP_011937.1. NM_001179200.1.

3D structure databases

ProteinModelPortaliP38793.
SMRiP38793. Positions 157-339.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi36502. 12 interactions.
DIPiDIP-2950N.
IntActiP38793. 2 interactions.
MINTiMINT-582793.

Proteomic databases

MaxQBiP38793.
PaxDbiP38793.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYHR070W; YHR070W; YHR070W.
GeneIDi856467.
KEGGisce:YHR070W.

Organism-specific databases

CYGDiYHR070w.
EuPathDBiFungiDB:YHR070W.
SGDiS000001112. TRM5.

Phylogenomic databases

eggNOGiCOG2520.
GeneTreeiENSGT00390000008208.
HOGENOMiHOG000195270.
InParanoidiP38793.
KOiK15429.
OMAiHEYVLDY.
OrthoDBiEOG75TMPT.

Enzyme and pathway databases

BioCyciMetaCyc:G3O-31120-MONOMER.
YEAST:G3O-31120-MONOMER.
BRENDAi2.1.1.228. 984.

Miscellaneous databases

NextBioi982126.
PROiP38793.

Family and domain databases

Gene3Di3.40.50.150. 2 hits.
HAMAPiMF_03152. TRM5.
InterProiIPR030382. MeTrfase_TRM5/TYW2.
IPR029063. SAM-dependent_MTases.
IPR025792. tRNA_Gua_MeTrfase_euk.
[Graphical view]
PfamiPF02475. Met_10. 1 hit.
[Graphical view]
SUPFAMiSSF53335. SSF53335. 2 hits.
PROSITEiPS51684. SAM_MT_TRM5_TYW2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  2. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  3. "A primordial tRNA modification required for the evolution of life?"
    Bjork G.R., Jacobsson K., Nilsson K., Johansson M.J., Bystroem A.S., Persson O.P.
    EMBO J. 20:231-239(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  4. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
  5. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  6. "Yeast mitochondrial initiator tRNA is methylated at guanosine 37 by the Trm5-encoded tRNA (guanine-N1-)-methyltransferase."
    Lee C., Kramer G., Graham D.E., Appling D.R.
    J. Biol. Chem. 282:27744-27753(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION, MUTAGENESIS OF 1-MET--GLN-33.
  7. "The use of Saccharomyces cerevisiae proteomic libraries to identify RNA-modifying proteins."
    Jackman J.E., Grayhack E.J., Phizicky E.M.
    Methods Mol. Biol. 488:383-393(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  8. "Retrograde nuclear import of tRNA precursors is required for modified base biogenesis in yeast."
    Ohira T., Suzuki T.
    Proc. Natl. Acad. Sci. U.S.A. 108:10502-10507(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION.

Entry informationi

Entry nameiTRM5_YEAST
AccessioniPrimary (citable) accession number: P38793
Secondary accession number(s): D3DL19
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: February 1, 1995
Last modified: July 22, 2015
This is version 124 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 4120 molecules/cell in log phase SD medium.1 Publication
It is unsure how the mitochondrial and cytoplasmic forms of this protein are produced. The cytoplasmic form may be produced by alternative initiation at a downstream in-frame AUG codon at position 34, lacking a mitochondrial transit peptide.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome VIII
    Yeast (Saccharomyces cerevisiae) chromosome VIII: entries and gene names

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.