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Protein

Deoxyhypusine synthase

Gene

DYS1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes the NAD-dependent oxidative cleavage of spermidine and the subsequent transfer of the butylamine moiety of spermidine to the epsilon-amino group of a specific lysine residue of the eIF-5A precursor protein to form the intermediate deoxyhypusine residue.

Miscellaneous

Present with 7600 molecules/cell in log phase SD medium.1 Publication

Catalytic activityi

[eIF5A-precursor]-lysine + spermidine = [eIF5A-precursor]-deoxyhypusine + propane-1,3-diamine.

Cofactori

Pathwayi: eIF5A hypusination

This protein is involved in the pathway eIF5A hypusination, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway eIF5A hypusination and in Protein modification.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei140NADBy similarity1
Binding sitei257NADBy similarity1
Binding sitei262SpermidineBy similarity1
Binding sitei304NAD; via amide nitrogenBy similarity1
Binding sitei309SpermidineBy similarity1
Active sitei350NucleophileBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi108 – 112NADBy similarity5
Nucleotide bindingi134 – 136NADBy similarity3
Nucleotide bindingi329 – 330NADBy similarity2
Nucleotide bindingi363 – 364NADBy similarity2

GO - Molecular functioni

  • deoxyhypusine synthase activity Source: SGD

GO - Biological processi

  • peptidyl-lysine modification to peptidyl-hypusine Source: SGD

Keywordsi

Molecular functionTransferase
Biological processHypusine biosynthesis
LigandNAD

Enzyme and pathway databases

BioCyciYEAST:YHR068W-MONOMER
ReactomeiR-SCE-204626 Hypusine synthesis from eIF5A-lysine
UniPathwayiUPA00354

Names & Taxonomyi

Protein namesi
Recommended name:
Deoxyhypusine synthase (EC:2.5.1.46)
Short name:
DHS
Gene namesi
Name:DYS1
Ordered Locus Names:YHR068W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome VIII

Organism-specific databases

EuPathDBiFungiDB:YHR068W
SGDiS000001110 DYS1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001344881 – 387Deoxyhypusine synthaseAdd BLAST387

Proteomic databases

MaxQBiP38791
PaxDbiP38791
PRIDEiP38791

PTM databases

iPTMnetiP38791

Interactioni

Subunit structurei

Homotetramer.

Binary interactionsi

WithEntry#Exp.IntActNotes
HYP2P233014EBI-5871,EBI-9033

Protein-protein interaction databases

BioGridi36500, 113 interactors
DIPiDIP-2788N
IntActiP38791, 7 interactors
MINTiP38791
STRINGi4932.YHR068W

Structurei

3D structure databases

ProteinModelPortaliP38791
SMRiP38791
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni139 – 140Spermidine bindingBy similarity2
Regioni335 – 337Spermidine bindingBy similarity3
Regioni344 – 350Spermidine bindingBy similarity7

Sequence similaritiesi

Belongs to the deoxyhypusine synthase family.Curated

Phylogenomic databases

GeneTreeiENSGT00390000008063
HOGENOMiHOG000228838
InParanoidiP38791
KOiK00809
OMAiGWEDSTM
OrthoDBiEOG092C3JEN

Family and domain databases

Gene3Di3.40.910.10, 1 hit
InterProiView protein in InterPro
IPR002773 Deoxyhypusine_synthase
IPR036982 Deoxyhypusine_synthase_sf
IPR029035 DHS-like_NAD/FAD-binding_dom
PANTHERiPTHR11703 PTHR11703, 1 hit
PfamiView protein in Pfam
PF01916 DS, 1 hit
SUPFAMiSSF52467 SSF52467, 1 hit
TIGRFAMsiTIGR00321 dhys, 1 hit

Sequencei

Sequence statusi: Complete.

P38791-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSDINEKLPE LLQDAVLKAS VPIPDDFVKV QGIDYSKPEA TNMRATDLIE
60 70 80 90 100
AMKTMGFQAS SVGTACEIID SMRSWRGKHI DELDDHEKKG CFDEEGYQKT
110 120 130 140 150
TIFMGYTSNL ISSGVRETLR YLVQHKMVDA VVTSAGGVEE DLIKCLAPTY
160 170 180 190 200
LGEFALKGKS LRDQGMNRIG NLLVPNDNYC KFEEWIVPIL DKMLEEQDEY
210 220 230 240 250
VKKHGADCLE ANQDVDSPIW TPSKMIDRFG KEINDESSVL YWAHKNKIPI
260 270 280 290 300
FCPSLTDGSI GDMLFFHTFK ASPKQLRVDI VGDIRKINSM SMAAYRAGMI
310 320 330 340 350
ILGGGLIKHH IANACLMRNG ADYAVYINTG QEYDGSDAGA RPDEAVSWGK
360 370 380
IKAEAKSVKL FADVTTVLPL IVAATFASGK PIKKVKN
Length:387
Mass (Da):42,892
Last modified:February 1, 1995 - v1
Checksum:i330BE1F5BF305F46
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D78185 Genomic DNA Translation: BAA11253.1
U00061 Genomic DNA Translation: AAB68377.1
AY558282 Genomic DNA Translation: AAS56608.1
BK006934 Genomic DNA Translation: DAA06761.1
PIRiS46698
RefSeqiNP_011935.1, NM_001179198.1

Genome annotation databases

EnsemblFungiiYHR068W; YHR068W; YHR068W
GeneIDi856465
KEGGisce:YHR068W

Similar proteinsi

Entry informationi

Entry nameiDHYS_YEAST
AccessioniPrimary (citable) accession number: P38791
Secondary accession number(s): D3DL17
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: February 1, 1995
Last modified: June 20, 2018
This is version 153 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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