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Protein

Deoxyhypusine synthase

Gene

DYS1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the NAD-dependent oxidative cleavage of spermidine and the subsequent transfer of the butylamine moiety of spermidine to the epsilon-amino group of a specific lysine residue of the eIF-5A precursor protein to form the intermediate deoxyhypusine residue.

Catalytic activityi

[eIF5A-precursor]-lysine + spermidine = [eIF5A-precursor]-deoxyhypusine + propane-1,3-diamine.

Cofactori

Pathwayi: eIF5A hypusination

This protein is involved in the pathway eIF5A hypusination, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway eIF5A hypusination and in Protein modification.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei140 – 1401NADBy similarity
Binding sitei257 – 2571NADBy similarity
Binding sitei262 – 2621SpermidineBy similarity
Binding sitei304 – 3041NAD; via amide nitrogenBy similarity
Binding sitei309 – 3091SpermidineBy similarity
Active sitei350 – 3501NucleophileBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi108 – 1125NADBy similarity
Nucleotide bindingi134 – 1363NADBy similarity
Nucleotide bindingi329 – 3302NADBy similarity
Nucleotide bindingi363 – 3642NADBy similarity

GO - Molecular functioni

  • deoxyhypusine synthase activity Source: SGD

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Hypusine biosynthesis

Keywords - Ligandi

NAD

Enzyme and pathway databases

BioCyciYEAST:YHR068W-MONOMER.
ReactomeiR-SCE-204626. Hypusine synthesis from eIF5A-lysine.
UniPathwayiUPA00354.

Names & Taxonomyi

Protein namesi
Recommended name:
Deoxyhypusine synthase (EC:2.5.1.46)
Short name:
DHS
Gene namesi
Name:DYS1
Ordered Locus Names:YHR068W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome VIII

Organism-specific databases

EuPathDBiFungiDB:YHR068W.
SGDiS000001110. DYS1.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 387387Deoxyhypusine synthasePRO_0000134488Add
BLAST

Proteomic databases

MaxQBiP38791.

PTM databases

iPTMnetiP38791.

Interactioni

Subunit structurei

Homotetramer.

Binary interactionsi

WithEntry#Exp.IntActNotes
HYP2P233014EBI-5871,EBI-9033

Protein-protein interaction databases

BioGridi36500. 84 interactions.
DIPiDIP-2788N.
IntActiP38791. 7 interactions.
MINTiMINT-516675.

Structurei

3D structure databases

ProteinModelPortaliP38791.
SMRiP38791. Positions 29-377.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni139 – 1402Spermidine bindingBy similarity
Regioni335 – 3373Spermidine bindingBy similarity
Regioni344 – 3507Spermidine bindingBy similarity

Sequence similaritiesi

Belongs to the deoxyhypusine synthase family.Curated

Phylogenomic databases

GeneTreeiENSGT00390000008063.
HOGENOMiHOG000228838.
InParanoidiP38791.
KOiK00809.
OMAiTYEQMVF.
OrthoDBiEOG092C3JEN.

Family and domain databases

Gene3Di3.40.910.10. 1 hit.
InterProiIPR002773. Deoxyhypusine_synthase.
IPR029035. DHS-like_NAD/FAD-binding_dom.
[Graphical view]
PANTHERiPTHR11703. PTHR11703. 2 hits.
PfamiPF01916. DS. 1 hit.
[Graphical view]
SUPFAMiSSF52467. SSF52467. 1 hit.
TIGRFAMsiTIGR00321. dhys. 1 hit.

Sequencei

Sequence statusi: Complete.

P38791-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSDINEKLPE LLQDAVLKAS VPIPDDFVKV QGIDYSKPEA TNMRATDLIE
60 70 80 90 100
AMKTMGFQAS SVGTACEIID SMRSWRGKHI DELDDHEKKG CFDEEGYQKT
110 120 130 140 150
TIFMGYTSNL ISSGVRETLR YLVQHKMVDA VVTSAGGVEE DLIKCLAPTY
160 170 180 190 200
LGEFALKGKS LRDQGMNRIG NLLVPNDNYC KFEEWIVPIL DKMLEEQDEY
210 220 230 240 250
VKKHGADCLE ANQDVDSPIW TPSKMIDRFG KEINDESSVL YWAHKNKIPI
260 270 280 290 300
FCPSLTDGSI GDMLFFHTFK ASPKQLRVDI VGDIRKINSM SMAAYRAGMI
310 320 330 340 350
ILGGGLIKHH IANACLMRNG ADYAVYINTG QEYDGSDAGA RPDEAVSWGK
360 370 380
IKAEAKSVKL FADVTTVLPL IVAATFASGK PIKKVKN
Length:387
Mass (Da):42,892
Last modified:February 1, 1995 - v1
Checksum:i330BE1F5BF305F46
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D78185 Genomic DNA. Translation: BAA11253.1.
U00061 Genomic DNA. Translation: AAB68377.1.
AY558282 Genomic DNA. Translation: AAS56608.1.
BK006934 Genomic DNA. Translation: DAA06761.1.
PIRiS46698.
RefSeqiNP_011935.1. NM_001179198.1.

Genome annotation databases

EnsemblFungiiYHR068W; YHR068W; YHR068W.
GeneIDi856465.
KEGGisce:YHR068W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D78185 Genomic DNA. Translation: BAA11253.1.
U00061 Genomic DNA. Translation: AAB68377.1.
AY558282 Genomic DNA. Translation: AAS56608.1.
BK006934 Genomic DNA. Translation: DAA06761.1.
PIRiS46698.
RefSeqiNP_011935.1. NM_001179198.1.

3D structure databases

ProteinModelPortaliP38791.
SMRiP38791. Positions 29-377.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi36500. 84 interactions.
DIPiDIP-2788N.
IntActiP38791. 7 interactions.
MINTiMINT-516675.

PTM databases

iPTMnetiP38791.

Proteomic databases

MaxQBiP38791.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYHR068W; YHR068W; YHR068W.
GeneIDi856465.
KEGGisce:YHR068W.

Organism-specific databases

EuPathDBiFungiDB:YHR068W.
SGDiS000001110. DYS1.

Phylogenomic databases

GeneTreeiENSGT00390000008063.
HOGENOMiHOG000228838.
InParanoidiP38791.
KOiK00809.
OMAiTYEQMVF.
OrthoDBiEOG092C3JEN.

Enzyme and pathway databases

UniPathwayiUPA00354.
BioCyciYEAST:YHR068W-MONOMER.
ReactomeiR-SCE-204626. Hypusine synthesis from eIF5A-lysine.

Miscellaneous databases

PROiP38791.

Family and domain databases

Gene3Di3.40.910.10. 1 hit.
InterProiIPR002773. Deoxyhypusine_synthase.
IPR029035. DHS-like_NAD/FAD-binding_dom.
[Graphical view]
PANTHERiPTHR11703. PTHR11703. 2 hits.
PfamiPF01916. DS. 1 hit.
[Graphical view]
SUPFAMiSSF52467. SSF52467. 1 hit.
TIGRFAMsiTIGR00321. dhys. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiDHYS_YEAST
AccessioniPrimary (citable) accession number: P38791
Secondary accession number(s): D3DL17
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: February 1, 1995
Last modified: September 7, 2016
This is version 141 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 7600 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome VIII
    Yeast (Saccharomyces cerevisiae) chromosome VIII: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.