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P38791 (DHYS_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 121. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Deoxyhypusine synthase

Short name=DHS
EC=2.5.1.46
Gene names
Name:DYS1
Ordered Locus Names:YHR068W
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length387 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Catalyzes the NAD-dependent oxidative cleavage of spermidine and the subsequent transfer of the butylamine moiety of spermidine to the epsilon-amino group of a specific lysine residue of the eIF-5A precursor protein to form the intermediate deoxyhypusine residue.

Catalytic activity

[eIF5A-precursor]-lysine + spermidine = [eIF5A-precursor]-deoxyhypusine + propane-1,3-diamine.

Cofactor

NAD.

Pathway

Protein modification; eIF5A hypusination.

Subunit structure

Homotetramer.

Miscellaneous

Present with 7600 molecules/cell in log phase SD medium.

Sequence similarities

Belongs to the deoxyhypusine synthase family.

Ontologies

Binary interactions

With

Entry

#Exp.

IntAct

Notes

HYP2P233014EBI-5871,EBI-9033

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 387387Deoxyhypusine synthase
PRO_0000134488

Regions

Nucleotide binding108 – 1125NAD By similarity
Nucleotide binding134 – 1363NAD By similarity
Nucleotide binding329 – 3302NAD By similarity
Nucleotide binding363 – 3642NAD By similarity
Region139 – 1402Spermidine binding By similarity
Region335 – 3373Spermidine binding By similarity
Region344 – 3507Spermidine binding By similarity

Sites

Active site3501Nucleophile By similarity
Binding site1401NAD By similarity
Binding site2571NAD By similarity
Binding site2621Spermidine By similarity
Binding site3041NAD; via amide nitrogen By similarity
Binding site3091Spermidine By similarity

Sequences

Sequence LengthMass (Da)Tools
P38791 [UniParc].

Last modified February 1, 1995. Version 1.
Checksum: 330BE1F5BF305F46

FASTA38742,892
        10         20         30         40         50         60 
MSDINEKLPE LLQDAVLKAS VPIPDDFVKV QGIDYSKPEA TNMRATDLIE AMKTMGFQAS 

        70         80         90        100        110        120 
SVGTACEIID SMRSWRGKHI DELDDHEKKG CFDEEGYQKT TIFMGYTSNL ISSGVRETLR 

       130        140        150        160        170        180 
YLVQHKMVDA VVTSAGGVEE DLIKCLAPTY LGEFALKGKS LRDQGMNRIG NLLVPNDNYC 

       190        200        210        220        230        240 
KFEEWIVPIL DKMLEEQDEY VKKHGADCLE ANQDVDSPIW TPSKMIDRFG KEINDESSVL 

       250        260        270        280        290        300 
YWAHKNKIPI FCPSLTDGSI GDMLFFHTFK ASPKQLRVDI VGDIRKINSM SMAAYRAGMI 

       310        320        330        340        350        360 
ILGGGLIKHH IANACLMRNG ADYAVYINTG QEYDGSDAGA RPDEAVSWGK IKAEAKSVKL 

       370        380 
FADVTTVLPL IVAATFASGK PIKKVKN 

« Hide

References

« Hide 'large scale' references
[1]"Deoxyhypusine synthase gene is essential for cell viability in the yeast Saccharomyces cerevisiae."
Sasaki K., Abid M.R., Miyazaki M.
FEBS Lett. 384:151-154(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: 131.
[2]"Complete nucleotide sequence of Saccharomyces cerevisiae chromosome VIII."
Johnston M., Andrews S., Brinkman R., Cooper J., Ding H., Dover J., Du Z., Favello A., Fulton L., Gattung S., Geisel C., Kirsten J., Kucaba T., Hillier L.W., Jier M., Johnston L., Langston Y., Latreille P. expand/collapse author list , Louis E.J., Macri C., Mardis E., Menezes S., Mouser L., Nhan M., Rifkin L., Riles L., St Peter H., Trevaskis E., Vaughan K., Vignati D., Wilcox L., Wohldman P., Waterston R., Wilson R., Vaudin M.
Science 265:2077-2082(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[3]Saccharomyces Genome Database
Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[4]"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae."
Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. expand/collapse author list , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J.
Genome Res. 17:536-543(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[5]"Identification of YHR068w in Saccharomyces cerevisiae chromosome VIII as a gene for deoxyhypusine synthase. Expression and characterization of the enzyme."
Kang K.R., Wolff E.C., Park M.H., Folk J.E., Chung S.I.
J. Biol. Chem. 270:18408-18412(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION, CHARACTERIZATION.
[6]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
D78185 Genomic DNA. Translation: BAA11253.1.
U00061 Genomic DNA. Translation: AAB68377.1.
AY558282 Genomic DNA. Translation: AAS56608.1.
BK006934 Genomic DNA. Translation: DAA06761.1.
PIRS46698.
RefSeqNP_011935.1. NM_001179198.1.

3D structure databases

ProteinModelPortalP38791.
SMRP38791. Positions 29-377.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid36500. 84 interactions.
DIPDIP-2788N.
IntActP38791. 7 interactions.
MINTMINT-516675.
STRING4932.YHR068W.

Proteomic databases

PaxDbP38791.
PeptideAtlasP38791.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYHR068W; YHR068W; YHR068W.
GeneID856465.
KEGGsce:YHR068W.

Organism-specific databases

CYGDYHR068w.
SGDS000001110. DYS1.

Phylogenomic databases

eggNOGCOG1899.
GeneTreeENSGT00390000008063.
HOGENOMHOG000228838.
KOK00809.
OMAVEIWADA.
OrthoDBEOG73BVPW.

Enzyme and pathway databases

BioCycYEAST:YHR068W-MONOMER.
UniPathwayUPA00354.

Gene expression databases

GenevestigatorP38791.

Family and domain databases

Gene3D3.40.910.10. 1 hit.
InterProIPR002773. Deoxyhypusine_synthase.
[Graphical view]
PANTHERPTHR11703. PTHR11703. 1 hit.
PfamPF01916. DS. 1 hit.
[Graphical view]
TIGRFAMsTIGR00321. dhys. 1 hit.
ProtoNetSearch...

Other

NextBio982120.
PROP38791.

Entry information

Entry nameDHYS_YEAST
AccessionPrimary (citable) accession number: P38791
Secondary accession number(s): D3DL17
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: February 1, 1995
Last modified: February 19, 2014
This is version 121 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome VIII

Yeast (Saccharomyces cerevisiae) chromosome VIII: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways