P38789 (SSF1_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ribosome biogenesis protein SSF1

Gene names

Name:	SSF1
Ordered Locus Names:	YHR066W

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › Saccharomyces cerevisiae

Protein attributes

Sequence length	453 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Required for biogenesis of the 60S ribosomal subunit. Ref.4 Ref.5
Subunit structure	Part of a complex that includes BRX1, RPF1, RPF2 and SSF1 or SSF2.
Subcellular location	Nucleus › nucleolus Ref.5.
Miscellaneous	Present with 8150 molecules/cell in log phase SD medium.
Sequence similarities	Contains 1 Brix domain.

Ontologies

Keywords
Biological process	Ribosome biogenesis
Cellular component	Nucleus
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	conjugation with cellular fusion Inferred from genetic interaction Ref.1. Source: SGD regulation of cell size Inferred from mutant phenotype PubMed 12089449. Source: SGD ribosomal large subunit assembly Inferred from mutant phenotype PubMed 11864606. Source: SGD
Cellular_component	nucleolus Inferred from direct assay PubMed 9611192. Source: SGD
Molecular_function	rRNA binding Inferred from physical interaction PubMed 11864606. Source: SGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 453	453	Ribosome biogenesis protein SSF1		PRO_0000120259
Regions
	Domain	26 – 348	323	Brix
Amino acid modifications
	Modified residue	301	1	Phosphothreonine Ref.7 Ref.8

Sequences

	Sequence		Length	Mass (Da)
	P38789 [UniParc]. Last modified February 1, 1995. Version 1. Checksum: 6F0344993BEF2217	FASTA	453	51,764
	10 20 30 40 50 60 MAKRRQKKRT HAQLTPEQEQ GIPKSMVIRV GQTSLANHSL NQLVKDFRQI MQPHTAIKLK 70 80 90 100 110 120 ERKSNKLKDF VVMCGPLGVT HLFMFTQSEK TGNVSLKIAR TPQGPTVTFQ VLDYSLGRDI 130 140 150 160 170 180 KKFLKRPKSL NNDDVLNPPL LVLNGFSTSK RSGEDDQDVN VEKVIVSMFQ NIFPPLNPAR 190 200 210 220 230 240 TSLNSIKRVF MINKDRETGE ISMRHYFIDI REVEISRNLK RLYKAKNNLS KTVPNLHRKE 250 260 270 280 290 300 DISSLILDHD LGAYTSESEI EDDAIVRVVD NQDVKAKHSQ SLKSQRTPVE KKDNKEREKE 310 320 330 340 350 360 TEEEDVEMEE PKPSENLQPT PRKKAIKLTE LGPRLTLKLV KIEEGICSGK VLHHEFVQKS 370 380 390 400 410 420 SEEIKALEKR HAAKMRLKEQ RKKEQEENIA KKKAVKDAKK QRKLERRKAR AAEGGEGQGK 430 440 450 DDAMSDDESS SSDSEHYGSV PEDLDSDLFS EVE

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"An essential gene pair in Saccharomyces cerevisiae with a potential role in mating." Yu Y., Hirsch J.P. DNA Cell Biol. 14:411-418(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Complete nucleotide sequence of Saccharomyces cerevisiae chromosome VIII." Johnston M., Andrews S., Brinkman R., Cooper J., Ding H., Dover J., Du Z., Favello A., Fulton L., Gattung S., Geisel C., Kirsten J., Kucaba T., Hillier L.W., Jier M., Johnston L., Langston Y., Latreille P. , Louis E.J., Macri C., Mardis E., Menezes S., Mouser L., Nhan M., Rifkin L., Riles L., St Peter H., Trevaskis E., Vaughan K., Vignati D., Wilcox L., Wohldman P., Waterston R., Wilson R., Vaudin M. Science 265:2077-2082(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[3]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[4]	"Ssf1p prevents premature processing of an early pre-60S ribosomal particle." Fatica A., Cronshaw A.D., Dlakic M., Tollervey D. Mol. Cell 9:341-351(2002) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
[5]	"Functional analysis in yeast of the Brix protein superfamily involved in the biogenesis of ribosomes." Bogengruber E., Briza P., Doppler E., Wimmer H., Koller L., Fasiolo F., Senger B., Hegemann J.H., Breitenbach M. FEMS Yeast Res. 3:35-43(2003) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION.
[6]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[7]	"Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae." Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P. J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-301, MASS SPECTROMETRY. Strain: ADR376.
[8]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-301, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U18113 Genomic DNA. Translation: AAA79980.1. U00061 Genomic DNA. Translation: AAB68379.1. BK006934 Genomic DNA. Translation: DAA06759.1.
PIR	S46700.
RefSeq	NP_011933.1. NM_001179196.1.
3D structure databases
ProteinModelPortal	P38789.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-6501N.
IntAct	P38789. 50 interactions.
MINT	MINT-612836.
STRING	4932.YHR066W.
Proteomic databases
PeptideAtlas	P38789.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YHR066W; YHR066W; YHR066W.
GeneID	856463.
KEGG	sce:YHR066W.
Organism-specific databases
CYGD	YHR066w.
SGD	S000001108. SSF1.
Phylogenomic databases
GeneTree	ENSGT00530000064158.
HOGENOM	HOG000210038.
KO	K14859.
OMA	KGPGHKP.
OrthoDB	EOG4FV08S.
Gene expression databases
Genevestigator	P38789.
GermOnline	YHR066W. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR007109. Brix. IPR026531. SSF1/SSF2. [Graphical view]
PANTHER	PTHR12661:SF2. PTHR12661:SF2. 1 hit.
Pfam	PF04427. Brix. 1 hit. [Graphical view]
SMART	SM00879. Brix. 1 hit. [Graphical view]
PROSITE	PS50833. BRIX. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	982114.

Entry information

Entry name

SSF1_YEAST

Accession

Primary (citable) accession number: P38789
Secondary accession number(s): D3DL15

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1995
Last sequence update:	February 1, 1995
Last modified:	April 3, 2013
This is version 99 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program