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P38788

- SSZ1_YEAST

UniProt

P38788 - SSZ1_YEAST

Protein

Ribosome-associated complex subunit SSZ1

Gene

SSZ1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 126 (01 Oct 2014)
      Sequence version 2 (11 Jul 2006)
      Previous versions | rss
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    Functioni

    Component of the ribosome-associated complex (RAC), a heterodimeric chaperone complex involved in regulation of accurate translation termination and in folding or maintaining nascent polypeptides in a folding-competent state. RAC stimulates the ATPase activity of the ribosome-associated pool of Hsp70-type chaperones SSB1/SSB2 that bind to the nascent polypeptide chain. SSZ1 is required for ZUO1 to function efficiently as a J-protein for SSB1/SSB2. Also involved in pleiotropic drug resistance by post-translational activation of transcription factor PDR1.3 Publications

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. protein binding Source: IntAct
    3. unfolded protein binding Source: SGD

    GO - Biological processi

    1. cytoplasmic translation Source: SGD
    2. regulation of translational fidelity Source: SGD
    3. rRNA processing Source: SGD
    4. translational frameshifting Source: SGD

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciYEAST:G3O-31115-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Ribosome-associated complex subunit SSZ1
    Alternative name(s):
    DnaK-related protein SSZ1
    Heat shock protein 70 homolog SSZ1
    Pleiotropic drug resistance protein 13
    Gene namesi
    Name:SSZ1
    Synonyms:PDR13
    Ordered Locus Names:YHR064C
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome VIII

    Organism-specific databases

    CYGDiYHR064c.
    SGDiS000001106. SSZ1.

    Subcellular locationi

    Cytoplasm 1 Publication

    GO - Cellular componenti

    1. cytoplasm Source: SGD
    2. polysome Source: SGD

    Keywords - Cellular componenti

    Cytoplasm

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi295 – 2951S → F: Increases readthrough in translation termination. 1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 538538Ribosome-associated complex subunit SSZ1PRO_0000078400Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei477 – 4771Phosphoserine3 Publications
    Modified residuei480 – 4801Phosphoserine4 Publications

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    MaxQBiP38788.
    PaxDbiP38788.
    PeptideAtlasiP38788.

    Expressioni

    Gene expression databases

    GenevestigatoriP38788.

    Interactioni

    Subunit structurei

    RAC is a heterodimer of the Hsp70/DnaK-type chaperone SSZ1 and the Hsp40/DnaJ-type chaperone ZUO1. RAC associates with ribosomes via ZUO1.1 Publication

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    ZUO1P325277EBI-24570,EBI-29684

    Protein-protein interaction databases

    BioGridi36496. 94 interactions.
    DIPiDIP-6305N.
    IntActiP38788. 56 interactions.
    MINTiMINT-671986.

    Structurei

    3D structure databases

    ProteinModelPortaliP38788.
    SMRiP38788. Positions 4-535.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni400 – 538139Peptide-binding domainSequence AnalysisAdd
    BLAST

    Domaini

    Neither ATP binding nor ATP hydrolysis is required for SSZ1 function.
    Does not seem to bind unfolded protein substrates, as its C-terminal putative peptide-binding domain is not required for its function.

    Sequence similaritiesi

    Belongs to the heat shock protein 70 family.Curated

    Phylogenomic databases

    eggNOGiCOG0443.
    GeneTreeiENSGT00750000118452.
    HOGENOMiHOG000184645.
    OrthoDBiEOG7P02SZ.

    Family and domain databases

    Gene3Di2.60.34.10. 1 hit.
    InterProiIPR029047. HSP70_peptide-bd.
    IPR013126. Hsp_70_fam.
    [Graphical view]
    PfamiPF00012. HSP70. 1 hit.
    [Graphical view]
    PRINTSiPR00301. HEATSHOCK70.
    SUPFAMiSSF100920. SSF100920. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    P38788-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSSPVIGITF GNTSSSIAYI NPKNDVDVIA NPDGERAIPS ALSYVGEDEY    50
    HGGQALQQLI RNPKNTIINF RDFIGLPFDK CDVSKCANGA PAVEVDGKVG 100
    FVISRGEGKE EKLTVDEVVS RHLNRLKLAA EDYIGSAVKE AVLTVPTNFS 150
    EEQKTALKAS AAKIGLQIVQ FINEPSAALL AHAEQFPFEK DVNVVVADFG 200
    GIRSDAAVIA VRNGIFTILA TAHDLSLGGD NLDTELVEYF ASEFQKKYQA 250
    NPRKNARSLA KLKANSSITK KTLSNATSAT ISIDSLADGF DYHASINRMR 300
    YELVANKVFA QFSSFVDSVI AKAELDPLDI DAVLLTGGVS FTPKLTTNLE 350
    YTLPESVEIL GPQNKNASNN PNELAASGAA LQARLISDYD ADELAEALQP 400
    VIVNTPHLKK PIGLIGAKGE FHPVLLAETS FPVQKKLTLK QAKGDFLIGV 450
    YEGDHHIEEK TLEPIPKEEN AEEDDESEWS DDEPEVVREK LYTLGTKLME 500
    LGIKNANGVE IIFNINKDGA LRVTARDLKT GNAVKGEL 538
    Length:538
    Mass (Da):58,238
    Last modified:July 11, 2006 - v2
    Checksum:i07B1A409B213E0B4
    GO

    Sequence cautioni

    The sequence AAB68391.1 differs from that shown. Reason: Erroneous initiation.
    The sequence AAT93146.1 differs from that shown. Reason: Erroneous initiation.

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti208 – 2081V → A in AAT93146. (PubMed:17322287)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U00061 Genomic DNA. Translation: AAB68391.1. Different initiation.
    AY693127 Genomic DNA. Translation: AAT93146.1. Different initiation.
    AY389300 mRNA. Translation: AAQ97232.1.
    AY389301 mRNA. Translation: AAQ97233.1.
    BK006934 Genomic DNA. Translation: DAA06757.1.
    PIRiS46712.
    RefSeqiNP_011931.2. NM_001179194.1.

    Genome annotation databases

    EnsemblFungiiYHR064C; YHR064C; YHR064C.
    GeneIDi856461.
    KEGGisce:YHR064C.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U00061 Genomic DNA. Translation: AAB68391.1 . Different initiation.
    AY693127 Genomic DNA. Translation: AAT93146.1 . Different initiation.
    AY389300 mRNA. Translation: AAQ97232.1 .
    AY389301 mRNA. Translation: AAQ97233.1 .
    BK006934 Genomic DNA. Translation: DAA06757.1 .
    PIRi S46712.
    RefSeqi NP_011931.2. NM_001179194.1.

    3D structure databases

    ProteinModelPortali P38788.
    SMRi P38788. Positions 4-535.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 36496. 94 interactions.
    DIPi DIP-6305N.
    IntActi P38788. 56 interactions.
    MINTi MINT-671986.

    Proteomic databases

    MaxQBi P38788.
    PaxDbi P38788.
    PeptideAtlasi P38788.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YHR064C ; YHR064C ; YHR064C .
    GeneIDi 856461.
    KEGGi sce:YHR064C.

    Organism-specific databases

    CYGDi YHR064c.
    SGDi S000001106. SSZ1.

    Phylogenomic databases

    eggNOGi COG0443.
    GeneTreei ENSGT00750000118452.
    HOGENOMi HOG000184645.
    OrthoDBi EOG7P02SZ.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-31115-MONOMER.

    Miscellaneous databases

    NextBioi 982108.

    Gene expression databases

    Genevestigatori P38788.

    Family and domain databases

    Gene3Di 2.60.34.10. 1 hit.
    InterProi IPR029047. HSP70_peptide-bd.
    IPR013126. Hsp_70_fam.
    [Graphical view ]
    Pfami PF00012. HSP70. 1 hit.
    [Graphical view ]
    PRINTSi PR00301. HEATSHOCK70.
    SUPFAMi SSF100920. SSF100920. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    2. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    3. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    4. "Verification of 3' and 5' ends of S. cerevisiae transcripts."
      Kennedy M.C., Dietrich F.S.
      Submitted (SEP-2003) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1-72 AND 504-538.
      Strain: ATCC 204511 / S288c / AB972.
    5. "Linking genome and proteome by mass spectrometry: large-scale identification of yeast proteins from two dimensional gels."
      Shevchenko A., Jensen O.N., Podtelejnikov A.V., Sagliocco F., Wilm M., Vorm O., Mortensen P., Shevchenko A., Boucherie H., Mann M.
      Proc. Natl. Acad. Sci. U.S.A. 93:14440-14445(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY.
    6. "Yeast Pdr13p and Zuo1p molecular chaperones are new functional Hsp70 and Hsp40 partners."
      Michimoto T., Aoki T., Toh-e A., Kikuchi Y.
      Gene 257:131-137(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH ZUO1.
    7. "RAC, a stable ribosome-associated complex in yeast formed by the DnaK-DnaJ homologs Ssz1p and zuotin."
      Gautschi M., Lilie H., Fuenfschilling U., Mun A., Ross S., Lithgow T., Ruecknagel P., Rospert S.
      Proc. Natl. Acad. Sci. U.S.A. 98:3762-3767(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION IN RAC.
    8. "The in vivo function of the ribosome-associated Hsp70, Ssz1, does not require its putative peptide-binding domain."
      Hundley H., Eisenman H., Walter W., Evans T., Hotokezaka Y., Wiedmann M., Craig E.A.
      Proc. Natl. Acad. Sci. U.S.A. 99:4203-4208(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    9. "Sequencing and comparison of yeast species to identify genes and regulatory elements."
      Kellis M., Patterson N., Endrizzi M., Birren B.W., Lander E.S.
      Nature 423:241-254(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION OF PROBABLE INITIATION SITE.
    10. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
    11. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
    12. "The ribosome-bound chaperones RAC and Ssb1/2p are required for accurate translation in Saccharomyces cerevisiae."
      Rakwalska M., Rospert S.
      Mol. Cell. Biol. 24:9186-9197(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, MUTAGENESIS OF SER-295.
    13. "The Hsp70 Ssz1 modulates the function of the ribosome-associated J-protein Zuo1."
      Huang P., Gautschi M., Walter W., Rospert S., Craig E.A.
      Nat. Struct. Mol. Biol. 12:497-504(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, ATP-BINDING.
    14. "Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
      Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
      J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-477 AND SER-480, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Strain: ADR376.
    15. "Analysis of phosphorylation sites on proteins from Saccharomyces cerevisiae by electron transfer dissociation (ETD) mass spectrometry."
      Chi A., Huttenhower C., Geer L.Y., Coon J.J., Syka J.E.P., Bai D.L., Shabanowitz J., Burke D.J., Troyanskaya O.G., Hunt D.F.
      Proc. Natl. Acad. Sci. U.S.A. 104:2193-2198(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-480, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    16. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
      Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
      Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-477 AND SER-480, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    17. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
      Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
      Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-477 AND SER-480, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiSSZ1_YEAST
    AccessioniPrimary (citable) accession number: P38788
    Secondary accession number(s): D3DL13
    , Q6B1F3, Q6TQT7, Q6TQT8
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 1, 1995
    Last sequence update: July 11, 2006
    Last modified: October 1, 2014
    This is version 126 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 73600 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families
    2. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    3. Yeast chromosome VIII
      Yeast (Saccharomyces cerevisiae) chromosome VIII: entries and gene names

    External Data

    Dasty 3