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P38788

- SSZ1_YEAST

UniProt

P38788 - SSZ1_YEAST

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Protein

Ribosome-associated complex subunit SSZ1

Gene
SSZ1, PDR13, YHR064C
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Component of the ribosome-associated complex (RAC), a heterodimeric chaperone complex involved in regulation of accurate translation termination and in folding or maintaining nascent polypeptides in a folding-competent state. RAC stimulates the ATPase activity of the ribosome-associated pool of Hsp70-type chaperones SSB1/SSB2 that bind to the nascent polypeptide chain. SSZ1 is required for ZUO1 to function efficiently as a J-protein for SSB1/SSB2. Also involved in pleiotropic drug resistance by post-translational activation of transcription factor PDR1.3 Publications

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. protein binding Source: IntAct
  3. unfolded protein binding Source: SGD

GO - Biological processi

  1. cytoplasmic translation Source: SGD
  2. regulation of translational fidelity Source: SGD
  3. rRNA processing Source: SGD
  4. translational frameshifting Source: SGD
Complete GO annotation...

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-31115-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Ribosome-associated complex subunit SSZ1
Alternative name(s):
DnaK-related protein SSZ1
Heat shock protein 70 homolog SSZ1
Pleiotropic drug resistance protein 13
Gene namesi
Name:SSZ1
Synonyms:PDR13
Ordered Locus Names:YHR064C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome VIII

Organism-specific databases

CYGDiYHR064c.
SGDiS000001106. SSZ1.

Subcellular locationi

Cytoplasm 1 Publication

GO - Cellular componenti

  1. cytoplasm Source: SGD
  2. polysome Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi295 – 2951S → F: Increases readthrough in translation termination. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 538538Ribosome-associated complex subunit SSZ1PRO_0000078400Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei477 – 4771Phosphoserine3 Publications
Modified residuei480 – 4801Phosphoserine4 Publications

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP38788.
PaxDbiP38788.
PeptideAtlasiP38788.

Expressioni

Gene expression databases

GenevestigatoriP38788.

Interactioni

Subunit structurei

RAC is a heterodimer of the Hsp70/DnaK-type chaperone SSZ1 and the Hsp40/DnaJ-type chaperone ZUO1. RAC associates with ribosomes via ZUO1.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
ZUO1P325277EBI-24570,EBI-29684

Protein-protein interaction databases

BioGridi36496. 94 interactions.
DIPiDIP-6305N.
IntActiP38788. 56 interactions.
MINTiMINT-671986.

Structurei

3D structure databases

ProteinModelPortaliP38788.
SMRiP38788. Positions 4-535.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni400 – 538139Peptide-binding domain Reviewed predictionAdd
BLAST

Domaini

Neither ATP binding nor ATP hydrolysis is required for SSZ1 function.
Does not seem to bind unfolded protein substrates, as its C-terminal putative peptide-binding domain is not required for its function.

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0443.
GeneTreeiENSGT00750000118452.
HOGENOMiHOG000184645.
OrthoDBiEOG7P02SZ.

Family and domain databases

Gene3Di2.60.34.10. 1 hit.
InterProiIPR029047. HSP70_peptide-bd.
IPR013126. Hsp_70_fam.
[Graphical view]
PfamiPF00012. HSP70. 1 hit.
[Graphical view]
PRINTSiPR00301. HEATSHOCK70.
SUPFAMiSSF100920. SSF100920. 1 hit.

Sequencei

Sequence statusi: Complete.

P38788-1 [UniParc]FASTAAdd to Basket

« Hide

MSSPVIGITF GNTSSSIAYI NPKNDVDVIA NPDGERAIPS ALSYVGEDEY    50
HGGQALQQLI RNPKNTIINF RDFIGLPFDK CDVSKCANGA PAVEVDGKVG 100
FVISRGEGKE EKLTVDEVVS RHLNRLKLAA EDYIGSAVKE AVLTVPTNFS 150
EEQKTALKAS AAKIGLQIVQ FINEPSAALL AHAEQFPFEK DVNVVVADFG 200
GIRSDAAVIA VRNGIFTILA TAHDLSLGGD NLDTELVEYF ASEFQKKYQA 250
NPRKNARSLA KLKANSSITK KTLSNATSAT ISIDSLADGF DYHASINRMR 300
YELVANKVFA QFSSFVDSVI AKAELDPLDI DAVLLTGGVS FTPKLTTNLE 350
YTLPESVEIL GPQNKNASNN PNELAASGAA LQARLISDYD ADELAEALQP 400
VIVNTPHLKK PIGLIGAKGE FHPVLLAETS FPVQKKLTLK QAKGDFLIGV 450
YEGDHHIEEK TLEPIPKEEN AEEDDESEWS DDEPEVVREK LYTLGTKLME 500
LGIKNANGVE IIFNINKDGA LRVTARDLKT GNAVKGEL 538
Length:538
Mass (Da):58,238
Last modified:July 11, 2006 - v2
Checksum:i07B1A409B213E0B4
GO

Sequence cautioni

The sequence AAB68391.1 differs from that shown. Reason: Erroneous initiation.
The sequence AAT93146.1 differs from that shown. Reason: Erroneous initiation.

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti208 – 2081V → A in AAT93146. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U00061 Genomic DNA. Translation: AAB68391.1. Different initiation.
AY693127 Genomic DNA. Translation: AAT93146.1. Different initiation.
AY389300 mRNA. Translation: AAQ97232.1.
AY389301 mRNA. Translation: AAQ97233.1.
BK006934 Genomic DNA. Translation: DAA06757.1.
PIRiS46712.
RefSeqiNP_011931.2. NM_001179194.1.

Genome annotation databases

EnsemblFungiiYHR064C; YHR064C; YHR064C.
GeneIDi856461.
KEGGisce:YHR064C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U00061 Genomic DNA. Translation: AAB68391.1 . Different initiation.
AY693127 Genomic DNA. Translation: AAT93146.1 . Different initiation.
AY389300 mRNA. Translation: AAQ97232.1 .
AY389301 mRNA. Translation: AAQ97233.1 .
BK006934 Genomic DNA. Translation: DAA06757.1 .
PIRi S46712.
RefSeqi NP_011931.2. NM_001179194.1.

3D structure databases

ProteinModelPortali P38788.
SMRi P38788. Positions 4-535.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 36496. 94 interactions.
DIPi DIP-6305N.
IntActi P38788. 56 interactions.
MINTi MINT-671986.

Proteomic databases

MaxQBi P38788.
PaxDbi P38788.
PeptideAtlasi P38788.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YHR064C ; YHR064C ; YHR064C .
GeneIDi 856461.
KEGGi sce:YHR064C.

Organism-specific databases

CYGDi YHR064c.
SGDi S000001106. SSZ1.

Phylogenomic databases

eggNOGi COG0443.
GeneTreei ENSGT00750000118452.
HOGENOMi HOG000184645.
OrthoDBi EOG7P02SZ.

Enzyme and pathway databases

BioCyci YEAST:G3O-31115-MONOMER.

Miscellaneous databases

NextBioi 982108.

Gene expression databases

Genevestigatori P38788.

Family and domain databases

Gene3Di 2.60.34.10. 1 hit.
InterProi IPR029047. HSP70_peptide-bd.
IPR013126. Hsp_70_fam.
[Graphical view ]
Pfami PF00012. HSP70. 1 hit.
[Graphical view ]
PRINTSi PR00301. HEATSHOCK70.
SUPFAMi SSF100920. SSF100920. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  2. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  3. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  4. "Verification of 3' and 5' ends of S. cerevisiae transcripts."
    Kennedy M.C., Dietrich F.S.
    Submitted (SEP-2003) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1-72 AND 504-538.
    Strain: ATCC 204511 / S288c / AB972.
  5. "Linking genome and proteome by mass spectrometry: large-scale identification of yeast proteins from two dimensional gels."
    Shevchenko A., Jensen O.N., Podtelejnikov A.V., Sagliocco F., Wilm M., Vorm O., Mortensen P., Shevchenko A., Boucherie H., Mann M.
    Proc. Natl. Acad. Sci. U.S.A. 93:14440-14445(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY.
  6. "Yeast Pdr13p and Zuo1p molecular chaperones are new functional Hsp70 and Hsp40 partners."
    Michimoto T., Aoki T., Toh-e A., Kikuchi Y.
    Gene 257:131-137(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH ZUO1.
  7. "RAC, a stable ribosome-associated complex in yeast formed by the DnaK-DnaJ homologs Ssz1p and zuotin."
    Gautschi M., Lilie H., Fuenfschilling U., Mun A., Ross S., Lithgow T., Ruecknagel P., Rospert S.
    Proc. Natl. Acad. Sci. U.S.A. 98:3762-3767(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION IN RAC.
  8. "The in vivo function of the ribosome-associated Hsp70, Ssz1, does not require its putative peptide-binding domain."
    Hundley H., Eisenman H., Walter W., Evans T., Hotokezaka Y., Wiedmann M., Craig E.A.
    Proc. Natl. Acad. Sci. U.S.A. 99:4203-4208(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  9. "Sequencing and comparison of yeast species to identify genes and regulatory elements."
    Kellis M., Patterson N., Endrizzi M., Birren B.W., Lander E.S.
    Nature 423:241-254(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION OF PROBABLE INITIATION SITE.
  10. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
  11. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  12. "The ribosome-bound chaperones RAC and Ssb1/2p are required for accurate translation in Saccharomyces cerevisiae."
    Rakwalska M., Rospert S.
    Mol. Cell. Biol. 24:9186-9197(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, MUTAGENESIS OF SER-295.
  13. "The Hsp70 Ssz1 modulates the function of the ribosome-associated J-protein Zuo1."
    Huang P., Gautschi M., Walter W., Rospert S., Craig E.A.
    Nat. Struct. Mol. Biol. 12:497-504(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, ATP-BINDING.
  14. "Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
    Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
    J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-477 AND SER-480, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Strain: ADR376.
  15. "Analysis of phosphorylation sites on proteins from Saccharomyces cerevisiae by electron transfer dissociation (ETD) mass spectrometry."
    Chi A., Huttenhower C., Geer L.Y., Coon J.J., Syka J.E.P., Bai D.L., Shabanowitz J., Burke D.J., Troyanskaya O.G., Hunt D.F.
    Proc. Natl. Acad. Sci. U.S.A. 104:2193-2198(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-480, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  16. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
    Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
    Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-477 AND SER-480, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  17. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
    Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
    Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-477 AND SER-480, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiSSZ1_YEAST
AccessioniPrimary (citable) accession number: P38788
Secondary accession number(s): D3DL13
, Q6B1F3, Q6TQT7, Q6TQT8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: July 11, 2006
Last modified: June 11, 2014
This is version 125 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 73600 molecules/cell in log phase SD medium.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome VIII
    Yeast (Saccharomyces cerevisiae) chromosome VIII: entries and gene names

External Data

Dasty 3

Similar proteinsi