Reviewed, UniProtKB/Swiss-Prot P38787 (PANE_YEAST)

Names and origin

Protein names

Recommended name:
    2-dehydropantoate 2-reductase
    EC=1.1.1.169
Alternative name(s):
    Ketopantoate reductase
      Short name=KPA reductase
      Short name=KPR

Gene names

Name:	PAN5
Ordered Locus Names:	YHR063C

Organism

Saccharomyces cerevisiae (Baker's yeast) [Complete proteome]

Taxonomic identifier

4932 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	379 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Catalyzes the NADPH-dependent reduction of ketopantoate into pantoic acid By similarity.
Catalytic activity	(R)-pantoate + NADP(+) = 2-dehydropantoate + NADPH.
Pathway	Cofactor biosynthesis; pantothenate biosynthesis; pantoate from 3-methyl-2-oxobutanoate: step 2/2.
Miscellaneous	Present with 2600 molecules/cell in log phase SD medium.
Sequence similarities	Belongs to the ketopantoate reductase family.

Ontologies

Keywords
Biological process	Pantothenate biosynthesis
Ligand	NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW pantothenate biosynthetic process Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: InterPro soluble fraction Inferred from direct assay. Source: SGD
Molecular function	2-dehydropantoate 2-reductase activity Inferred from electronic annotation. Source: EC NADP binding Inferred from electronic annotation. Source: InterPro protein binding Inferred from physical interaction. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
AUS1	Q08409	1	EBI-12913,EBI-35723
PRP40	P33203	1	EBI-12913,EBI-701
SSM4	P40318	1	EBI-12913,EBI-18208

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 379	379	2-dehydropantoate 2-reductase		PRO_0000157328
Regions
	Nucleotide binding	13 – 18	6	NADP By similarity
Sites
	Active site	224	1	Proton donor By similarity
	Binding site	119	1	NADP; via amide nitrogen By similarity
	Binding site	119	1	Substrate By similarity
	Binding site	228	1	Substrate By similarity
	Binding site	232	1	Substrate By similarity
	Binding site	316	1	Substrate By similarity
	Binding site	328	1	NADP By similarity

Sequences

	Sequence		Length	Mass (Da)
	P38787-1 [UniParc]. Last modified February 1, 1995. Version 1. Checksum: 22BCAB56B2AE1AF3	FASTA	379	42,821
	10 20 30 40 50 60 MTAPHRSTIH ILGLGAMGTV LAVDLLRFTN ALVVPLFRSQ ERLAQFQKTN GNNISIRKLY 70 80 90 100 110 120 LEGSPIFSYP VEKCECPETF SKKPIDNLVV TTKTYQTKEA LAPYLPYINK NTNLILIQNG 130 140 150 160 170 180 LGVLELLREE IFTDSKNRPH LFQGVISHGV YQDKAGVFNH AGWAGMKIAK LPWTEEEMIQ 190 200 210 220 230 240 KKSVVEDDAA NNSLVKLLTE PKFAKEFGIE HSTYQEMLFG QLFKFLVNAC MNPVTAILDC 250 260 270 280 290 300 VNGEMKASCG PVFTSIIDEC LQILRVAYRP LFQYHEKYSG NEEYPEMDVN AVLTTDNMVS 310 320 330 340 350 360 EVTRIGCDIN SRNSSSMRQD TLFLRDIEIE YINGYVVKLA DNLNLDPNCC KVNKTIGELA 370 TMRLALNRSR SINGDWRKD

References

[1]	"Complete nucleotide sequence of Saccharomyces cerevisiae chromosome VIII." Johnston M., Andrews S., Brinkman R., Cooper J., Ding H., Dover J., Du Z., Favello A., Fulton L., Gattung S., Geisel C., Kirsten J., Kucaba T., Hillier L.W., Jier M., Johnston L., Langston Y., Latreille P. , Louis E.J., Macri C., Mardis E., Menezes S., Mouser L., Nhan M., Rifkin L., Riles L., St Peter H., Trevaskis E., Vaughan K., Vignati D., Wilcox L., Wohldman P., Waterston R., Wilson R., Vaudin M. Science 265:2077-2082(1994) [PubMed: 8091229] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[2]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	U00061 Genomic DNA. Translation: AAB68390.1.
PIR	S46711.
RefSeq	NP_011930.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
DIP	DIP:5591N.
IntAct	P38787.
Proteomic databases
PeptideAtlas	P38787.
Genome annotation databases
Ensembl	YHR063C. Saccharomyces cerevisiae. [Contig view]
GeneID	856460.
GenomeReviews	Gene locus YHR063C in contig U00093_GR.
KEGG	sce:YHR063C.
NMPDR	fig|4932.3.peg.3083.
Organism-specific databases
CYGD	YHR063c.
SGD	S000001105. PAN5.
Yeast-GFP	Search...
Phylogenomic databases
HOGENOM	P38787.
Gene expression databases
GermOnline	YHR063C. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR003710. ApbA. IPR013752. ApbA_C. IPR013332. ApbA_N. IPR013328. DHase_multihelical. IPR016040. NAD(P)-bd. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit. G3DSA:1.10.1040.10. Opine_DH. 1 hit.
PANTHER	PTHR21708:SF21. ApbA. 1 hit.
Pfam	PF02558. ApbA. 1 hit. PF08546. ApbA_C. 1 hit. [Graphical view]
TIGRFAMs	TIGR00745. apbA_panE. 1 hit.
ProtoNet	Search...
Other Resources
LinkHub	P38787.
NextBio	982105.

Entry information

Entry name

PANE_YEAST

Accession

Primary (citable) accession number: P38787

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1995
Last sequence update:	February 1, 1995
Last modified:	November 25, 2008
This is version 61 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)