P38787 (PANE_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
2-dehydropantoate 2-reductase
EC=1.1.1.169

Alternative name(s):
Ketopantoate reductase
Short name=KPA reductase
Short name=KPR

Gene names

Name:	PAN5
Ordered Locus Names:	YHR063C

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › Saccharomyces cerevisiae

Protein attributes

Sequence length	379 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Catalyzes the NADPH-dependent reduction of ketopantoate into pantoic acid By similarity.
Catalytic activity	(R)-pantoate + NADP+ = 2-dehydropantoate + NADPH.
Pathway	Cofactor biosynthesis; (R)-pantothenate biosynthesis; (R)-pantoate from 3-methyl-2-oxobutanoate: step 2/2.
Miscellaneous	Present with 2600 molecules/cell in log phase SD medium.
Sequence similarities	Belongs to the ketopantoate reductase family.

Ontologies

Keywords
Biological process	Pantothenate biosynthesis
Ligand	NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	pantothenate biosynthetic process Inferred from sequence or structural similarity PubMed 11154694. Source: SGD
Cellular_component	cytoplasm Inferred from direct assay PubMed 14562095. Source: SGD
Molecular_function	2-dehydropantoate 2-reductase activity Inferred from sequence or structural similarity PubMed 11154694. Source: SGD NADP binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
itself		1	EBI-12913,EBI-12913
AUS1	Q08409	1	EBI-12913,EBI-35723
PRP40	P33203	1	EBI-12913,EBI-701
SSM4	P40318	1	EBI-12913,EBI-18208

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 379	379	2-dehydropantoate 2-reductase		PRO_0000157328
Regions
	Nucleotide binding	13 – 18	6	NADP By similarity
Sites
	Active site	224	1	Proton donor By similarity
	Binding site	119	1	NADP; via amide nitrogen By similarity
	Binding site	119	1	Substrate By similarity
	Binding site	228	1	Substrate By similarity
	Binding site	232	1	Substrate By similarity
	Binding site	316	1	Substrate By similarity
	Binding site	328	1	NADP By similarity

Sequences

	Sequence		Length	Mass (Da)
	P38787 [UniParc]. Last modified February 1, 1995. Version 1. Checksum: 22BCAB56B2AE1AF3	FASTA	379	42,821
	10 20 30 40 50 60 MTAPHRSTIH ILGLGAMGTV LAVDLLRFTN ALVVPLFRSQ ERLAQFQKTN GNNISIRKLY 70 80 90 100 110 120 LEGSPIFSYP VEKCECPETF SKKPIDNLVV TTKTYQTKEA LAPYLPYINK NTNLILIQNG 130 140 150 160 170 180 LGVLELLREE IFTDSKNRPH LFQGVISHGV YQDKAGVFNH AGWAGMKIAK LPWTEEEMIQ 190 200 210 220 230 240 KKSVVEDDAA NNSLVKLLTE PKFAKEFGIE HSTYQEMLFG QLFKFLVNAC MNPVTAILDC 250 260 270 280 290 300 VNGEMKASCG PVFTSIIDEC LQILRVAYRP LFQYHEKYSG NEEYPEMDVN AVLTTDNMVS 310 320 330 340 350 360 EVTRIGCDIN SRNSSSMRQD TLFLRDIEIE YINGYVVKLA DNLNLDPNCC KVNKTIGELA 370 TMRLALNRSR SINGDWRKD

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Complete nucleotide sequence of Saccharomyces cerevisiae chromosome VIII." Johnston M., Andrews S., Brinkman R., Cooper J., Ding H., Dover J., Du Z., Favello A., Fulton L., Gattung S., Geisel C., Kirsten J., Kucaba T., Hillier L.W., Jier M., Johnston L., Langston Y., Latreille P. , Louis E.J., Macri C., Mardis E., Menezes S., Mouser L., Nhan M., Rifkin L., Riles L., St Peter H., Trevaskis E., Vaughan K., Vignati D., Wilcox L., Wohldman P., Waterston R., Wilson R., Vaudin M. Science 265:2077-2082(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[2]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[3]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U00061 Genomic DNA. Translation: AAB68390.1. BK006934 Genomic DNA. Translation: DAA06756.1.
PIR	S46711.
RefSeq	NP_011930.1. NM_001179193.1.
3D structure databases
ProteinModelPortal	P38787.
SMR	P38787. Positions 86-346.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-5591N.
IntAct	P38787. 5 interactions.
MINT	MINT-502833.
STRING	4932.YHR063C.
Proteomic databases
PaxDb	P38787.
PeptideAtlas	P38787.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YHR063C; YHR063C; YHR063C.
GeneID	856460.
KEGG	sce:YHR063C.
Organism-specific databases
CYGD	YHR063c.
SGD	S000001105. PAN5.
Phylogenomic databases
eggNOG	COG1893.
HOGENOM	HOG000000875.
KO	K00077.
OMA	NSSSMRE.
OrthoDB	EOG4STWDS.
Enzyme and pathway databases
BioCyc	YEAST:MONOMER3O-214.
UniPathway	UPA00028; UER00004.
Gene expression databases
Genevestigator	P38787.
GermOnline	YHR063C. Saccharomyces cerevisiae.
Family and domain databases
Gene3D	1.10.1040.10. 1 hit. 3.40.50.720. 1 hit.
InterPro	IPR008927. 6-PGluconate_DH_C-like. IPR003710. ApbA. IPR013752. ApbA_C. IPR013332. ApbA_N. IPR013328. DH_multihelical. IPR016040. NAD(P)-bd_dom. [Graphical view]
Pfam	PF02558. ApbA. 1 hit. PF08546. ApbA_C. 1 hit. [Graphical view]
SUPFAM	SSF48179. 6DGDH_C_like. 1 hit.
TIGRFAMs	TIGR00745. apbA_panE. 1 hit.
ProtoNet	Search...
Other
NextBio	982105.

Entry information

Entry name

PANE_YEAST

Accession

Primary (citable) accession number: P38787
Secondary accession number(s): D3DL12

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1995
Last sequence update:	February 1, 1995
Last modified:	May 29, 2013
This is version 108 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program