P38786 (RPP1_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ribonuclease P/MRP protein subunit RPP1
EC=3.1.26.5

Alternative name(s):
RNA-processing protein RPP1
RNaseP/MRP 32.2 kDa subunit

Gene names

Name:	RPP1
Ordered Locus Names:	YHR062C

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › Saccharomyces cerevisiae

Protein attributes

Sequence length	293 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Component of ribonuclease P, a protein complex that generates mature tRNA molecules by cleaving their 5'-ends. Also a component of RNase MRP, which cleaves pre-rRNA sequences. Ref.1 Ref.5
Catalytic activity	Endonucleolytic cleavage of RNA, removing 5'-extranucleotides from tRNA precursor.
Subunit structure	Component of nuclear RNase P and RNase MRP complexes. RNase P consists of an RNA moiety and at least 9 protein subunits including POP1, POP3, POP4, POP5, POP6, POP7, POP8, RPP1 and RPR2. RNase MRP complex consists of an RNA moiety and at least 10 protein subunits including POP1, POP3, POP4, POP5, POP6, POP7, POP8, RMP1, RPP1 and SNM1, many of which are shared with the RNase P complex. Ref.1 Ref.5 Ref.8
Subcellular location	Nucleus Ref.6.
Miscellaneous	Present with 6300 molecules/cell in log phase SD medium.
Sequence similarities	Belongs to the eukaryotic/archaeal RNase P protein component 3 family.

Ontologies

Keywords
Biological process	rRNA processing tRNA processing
Cellular component	Nucleus
Molecular function	Hydrolase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	intronic box C/D snoRNA processing Inferred from direct assay PubMed 18713869. Source: SGD mRNA cleavage Inferred from physical interaction PubMed 14729943. Source: SGD rRNA processing Inferred from direct assay Ref.5. Source: SGD tRNA processing Inferred from direct assay Ref.5. Source: SGD
Cellular_component	nucleolar ribonuclease P complex Inferred from direct assay Ref.5. Source: SGD ribonuclease MRP complex Inferred from direct assay Ref.5. Source: SGD
Molecular_function	ribonuclease P activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 293	293	Ribonuclease P/MRP protein subunit RPP1		PRO_0000140034

Sequences

	Sequence		Length	Mass (Da)
	P38786 [UniParc]. Last modified February 1, 1995. Version 1. Checksum: E9C775D8C646B5C7	FASTA	293	32,224
	10 20 30 40 50 60 MLVDLNVPWP QNSYADKVTS QAVNNLIKTL STLHMLGYTH IAINFTVNHS EKFPNDVKLL 70 80 90 100 110 120 NPIDIKRRFG ELMDRTGLKL YSRITLIIDD PSKGQSLSKI SQAFDIVAAL PISEKGLTLS 130 140 150 160 170 180 TTNLDIDLLT FQYGSRLPTF LKHKSICSCV NRGVKLEIVY GYALRDVQAR RQFVSNVRSV 190 200 210 220 230 240 IRSSRSRGIV IGSGAMSPLE CRNILGVTSL IKNLGLPSDR CSKAMGDLAS LVLLNGRLRN 250 260 270 280 290 KSHKQTIVTG GGSGNGDDVV NDVQGIDDVQ TIKVVKRSMD AEQLGHASKR HKP

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Rpp1, an essential protein subunit of nuclear RNase P required for processing of precursor tRNA and 35S precursor rRNA in Saccharomyces cerevisiae." Stolc V., Altman S. Genes Dev. 11:2414-2425(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, SUBUNIT.
[2]	Erratum Stolc V., Altman S. Genes Dev. 11:2926-2937(1997) [PubMed] [Europe PMC] [Abstract]
[3]	"Complete nucleotide sequence of Saccharomyces cerevisiae chromosome VIII." Johnston M., Andrews S., Brinkman R., Cooper J., Ding H., Dover J., Du Z., Favello A., Fulton L., Gattung S., Geisel C., Kirsten J., Kucaba T., Hillier L.W., Jier M., Johnston L., Langston Y., Latreille P. , Louis E.J., Macri C., Mardis E., Menezes S., Mouser L., Nhan M., Rifkin L., Riles L., St Peter H., Trevaskis E., Vaughan K., Vignati D., Wilcox L., Wohldman P., Waterston R., Wilson R., Vaudin M. Science 265:2077-2082(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[4]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[5]	"Purification and characterization of the nuclear RNase P holoenzyme complex reveals extensive subunit overlap with RNase MRP." Chamberlain J.R., Lee Y., Lane W.S., Engelke D.R. Genes Dev. 12:1678-1690(1998) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, IDENTIFICATION IN THE RNASE P COMPLEX BY MASS SPECTROMETRY.
[6]	"Global analysis of protein localization in budding yeast." Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K. Nature 425:686-691(2003) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[7]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[8]	"Characterization and purification of Saccharomyces cerevisiae RNase MRP reveals a new unique protein component." Salinas K., Wierzbicki S., Zhou L., Schmitt M.E. J. Biol. Chem. 280:11352-11360(2005) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION IN THE RNASE MRP COMPLEX BY MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U95757 Genomic DNA. Translation: AAB96559.1. U00061 Genomic DNA. Translation: AAB68389.1. BK006934 Genomic DNA. Translation: DAA06755.1.
PIR	S46710.
RefSeq	NP_011929.1. NM_001179192.1.
3D structure databases
ProteinModelPortal	P38786.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-5590N.
IntAct	P38786. 6 interactions.
MINT	MINT-501586.
STRING	4932.YHR062C.
Proteomic databases
PaxDb	P38786.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YHR062C; YHR062C; YHR062C.
GeneID	856459.
KEGG	sce:YHR062C.
Organism-specific databases
CYGD	YHR062c.
SGD	S000001104. RPP1.
Phylogenomic databases
eggNOG	COG1603.
GeneTree	ENSGT00390000000883.
HOGENOM	HOG000157793.
KO	K03539.
OMA	RITILYV.
OrthoDB	EOG4KPXMF.
Gene expression databases
Genevestigator	P38786.
GermOnline	YHR062C. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR016195. Pol/histidinol_Pase-like. IPR002738. RNase_P_p30. [Graphical view]
PANTHER	PTHR13031. PTHR13031. 1 hit.
Pfam	PF01876. RNase_P_p30. 1 hit. [Graphical view]
SUPFAM	SSF89550. PHP-like. 1 hit.
ProtoNet	Search...
Other
NextBio	982102.

Entry information

Entry name

RPP1_YEAST

Accession

Primary (citable) accession number: P38786
Secondary accession number(s): D3DL11

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1995
Last sequence update:	February 1, 1995
Last modified:	April 3, 2013
This is version 103 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program