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Protein

Ribonuclease P/MRP protein subunit RPP1

Gene

RPP1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Component of ribonuclease P, a protein complex that generates mature tRNA molecules by cleaving their 5'-ends. Also a component of RNase MRP, which cleaves pre-rRNA sequences.2 Publications

Catalytic activityi

Endonucleolytic cleavage of RNA, removing 5'-extranucleotides from tRNA precursor.

GO - Molecular functioni

  1. ribonuclease P activity Source: UniProtKB-EC
  2. RNA binding Source: SGD

GO - Biological processi

  1. intronic box C/D snoRNA processing Source: SGD
  2. mRNA cleavage Source: SGD
  3. RNA phosphodiester bond hydrolysis Source: GOC
  4. RNA phosphodiester bond hydrolysis, endonucleolytic Source: GOC
  5. rRNA processing Source: SGD
  6. tRNA processing Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

rRNA processing, tRNA processing

Enzyme and pathway databases

BioCyciYEAST:YHR062C-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Ribonuclease P/MRP protein subunit RPP1 (EC:3.1.26.5)
Alternative name(s):
RNA-processing protein RPP1
RNaseP/MRP 32.2 kDa subunit
Gene namesi
Name:RPP1
Ordered Locus Names:YHR062C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome VIII

Organism-specific databases

CYGDiYHR062c.
SGDiS000001104. RPP1.

Subcellular locationi

Nucleus 1 Publication

GO - Cellular componenti

  1. cytosol Source: SGD
  2. nucleolar ribonuclease P complex Source: SGD
  3. nucleus Source: SGD
  4. ribonuclease MRP complex Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 293293Ribonuclease P/MRP protein subunit RPP1PRO_0000140034Add
BLAST

Proteomic databases

MaxQBiP38786.
PaxDbiP38786.

Expressioni

Gene expression databases

GenevestigatoriP38786.

Interactioni

Subunit structurei

Component of nuclear RNase P and RNase MRP complexes. RNase P consists of an RNA moiety and at least 9 protein subunits including POP1, POP3, POP4, POP5, POP6, POP7, POP8, RPP1 and RPR2. RNase MRP complex consists of an RNA moiety and at least 10 protein subunits including POP1, POP3, POP4, POP5, POP6, POP7, POP8, RMP1, RPP1 and SNM1, many of which are shared with the RNase P complex.3 Publications

Protein-protein interaction databases

BioGridi36494. 20 interactions.
DIPiDIP-5590N.
IntActiP38786. 6 interactions.
MINTiMINT-501586.
STRINGi4932.YHR062C.

Structurei

3D structure databases

ProteinModelPortaliP38786.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG1603.
GeneTreeiENSGT00390000000883.
HOGENOMiHOG000157793.
InParanoidiP38786.
KOiK03539.
OMAiRLYDIIA.
OrthoDBiEOG7X3R34.

Family and domain databases

InterProiIPR016195. Pol/histidinol_Pase-like.
IPR002738. RNase_P_p30.
[Graphical view]
PANTHERiPTHR13031. PTHR13031. 1 hit.
PfamiPF01876. RNase_P_p30. 1 hit.
[Graphical view]
SUPFAMiSSF89550. SSF89550. 1 hit.

Sequencei

Sequence statusi: Complete.

P38786-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MLVDLNVPWP QNSYADKVTS QAVNNLIKTL STLHMLGYTH IAINFTVNHS
60 70 80 90 100
EKFPNDVKLL NPIDIKRRFG ELMDRTGLKL YSRITLIIDD PSKGQSLSKI
110 120 130 140 150
SQAFDIVAAL PISEKGLTLS TTNLDIDLLT FQYGSRLPTF LKHKSICSCV
160 170 180 190 200
NRGVKLEIVY GYALRDVQAR RQFVSNVRSV IRSSRSRGIV IGSGAMSPLE
210 220 230 240 250
CRNILGVTSL IKNLGLPSDR CSKAMGDLAS LVLLNGRLRN KSHKQTIVTG
260 270 280 290
GGSGNGDDVV NDVQGIDDVQ TIKVVKRSMD AEQLGHASKR HKP
Length:293
Mass (Da):32,224
Last modified:February 1, 1995 - v1
Checksum:iE9C775D8C646B5C7
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U95757 Genomic DNA. Translation: AAB96559.1.
U00061 Genomic DNA. Translation: AAB68389.1.
BK006934 Genomic DNA. Translation: DAA06755.1.
PIRiS46710.
RefSeqiNP_011929.1. NM_001179192.1.

Genome annotation databases

EnsemblFungiiYHR062C; YHR062C; YHR062C.
GeneIDi856459.
KEGGisce:YHR062C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U95757 Genomic DNA. Translation: AAB96559.1.
U00061 Genomic DNA. Translation: AAB68389.1.
BK006934 Genomic DNA. Translation: DAA06755.1.
PIRiS46710.
RefSeqiNP_011929.1. NM_001179192.1.

3D structure databases

ProteinModelPortaliP38786.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi36494. 20 interactions.
DIPiDIP-5590N.
IntActiP38786. 6 interactions.
MINTiMINT-501586.
STRINGi4932.YHR062C.

Proteomic databases

MaxQBiP38786.
PaxDbiP38786.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYHR062C; YHR062C; YHR062C.
GeneIDi856459.
KEGGisce:YHR062C.

Organism-specific databases

CYGDiYHR062c.
SGDiS000001104. RPP1.

Phylogenomic databases

eggNOGiCOG1603.
GeneTreeiENSGT00390000000883.
HOGENOMiHOG000157793.
InParanoidiP38786.
KOiK03539.
OMAiRLYDIIA.
OrthoDBiEOG7X3R34.

Enzyme and pathway databases

BioCyciYEAST:YHR062C-MONOMER.

Miscellaneous databases

NextBioi982102.
PROiP38786.

Gene expression databases

GenevestigatoriP38786.

Family and domain databases

InterProiIPR016195. Pol/histidinol_Pase-like.
IPR002738. RNase_P_p30.
[Graphical view]
PANTHERiPTHR13031. PTHR13031. 1 hit.
PfamiPF01876. RNase_P_p30. 1 hit.
[Graphical view]
SUPFAMiSSF89550. SSF89550. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Rpp1, an essential protein subunit of nuclear RNase P required for processing of precursor tRNA and 35S precursor rRNA in Saccharomyces cerevisiae."
    Stolc V., Altman S.
    Genes Dev. 11:2414-2425(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, SUBUNIT.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  4. "Purification and characterization of the nuclear RNase P holoenzyme complex reveals extensive subunit overlap with RNase MRP."
    Chamberlain J.R., Lee Y., Lane W.S., Engelke D.R.
    Genes Dev. 12:1678-1690(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, IDENTIFICATION IN THE RNASE P COMPLEX BY MASS SPECTROMETRY.
  5. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
  6. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  7. "Characterization and purification of Saccharomyces cerevisiae RNase MRP reveals a new unique protein component."
    Salinas K., Wierzbicki S., Zhou L., Schmitt M.E.
    J. Biol. Chem. 280:11352-11360(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION IN THE RNASE MRP COMPLEX BY MASS SPECTROMETRY.

Entry informationi

Entry nameiRPP1_YEAST
AccessioniPrimary (citable) accession number: P38786
Secondary accession number(s): D3DL11
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: February 1, 1995
Last modified: January 7, 2015
This is version 117 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 6300 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome VIII
    Yeast (Saccharomyces cerevisiae) chromosome VIII: entries and gene names

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.