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Protein

Vacuolar ATPase assembly protein VMA22

Gene

VMA22

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Required for V-ATPase activity.

GO - Molecular functioni

  • unfolded protein binding Source: SGD

GO - Biological processi

  • vacuolar acidification Source: SGD
  • vacuolar proton-transporting V-type ATPase complex assembly Source: SGD
Complete GO annotation...

Enzyme and pathway databases

BioCyciYEAST:G3O-31113-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Vacuolar ATPase assembly protein VMA22
Gene namesi
Name:VMA22
Ordered Locus Names:YHR060W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome VIII

Organism-specific databases

EuPathDBiFungiDB:YHR060W.
SGDiS000001102. VMA22.

Subcellular locationi

GO - Cellular componenti

  • extrinsic component of endoplasmic reticulum membrane Source: SGD
  • Vma12-Vma22 assembly complex Source: SGD
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 181181Vacuolar ATPase assembly protein VMA22PRO_0000065876Add
BLAST
Isoform 2 (identifier: P38784-2)
Initiator methionineiRemovedCombined sources

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Isoform 2 (identifier: P38784-2)
Modified residuei2 – 21N-acetylalanineCombined sources

Keywords - PTMi

Acetylation

Proteomic databases

MaxQBiP38784.

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
VMA6P323664EBI-20329,EBI-20201

GO - Molecular functioni

  • unfolded protein binding Source: SGD

Protein-protein interaction databases

BioGridi36492. 51 interactions.
DIPiDIP-1736N.
IntActiP38784. 9 interactions.
MINTiMINT-386505.

Structurei

3D structure databases

ProteinModelPortaliP38784.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Phylogenomic databases

HOGENOMiHOG000000873.
InParanoidiP38784.
OMAiRANYHNK.
OrthoDBiEOG72ZCSC.

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative initiation. AlignAdd to basket

Isoform 1 (identifier: P38784-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSETRMAQNM DTTDEQYLRL IELLSNYDST LEQLQKGFQD GYIQLSRSNY
60 70 80 90 100
YNKDSLRGNY GEDYWDETYI GQLMATVEEK NSKVVVEIVK RKAQDKQEKK
110 120 130 140 150
EEEDNKLTQR KKGTKPEKQK TQSHKLKQDY DPILMFGGVL SVPSSLRQSQ
160 170 180
TSFKGCIPLI AQLINYKNEI LTLVETLSEQ E
Length:181
Mass (Da):21,078
Last modified:February 1, 1995 - v1
Checksum:iC1CB5DC8292D2734
GO
Isoform 2 (identifier: P38784-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-5: Missing.

Show »
Length:176
Mass (Da):20,473
Checksum:iB15B1BEB45347578
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 55Missing in isoform 2. CuratedVSP_058123

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U24501 Genomic DNA. Translation: AAA87458.1.
U00061 Genomic DNA. Translation: AAB68380.1.
BK006934 Genomic DNA. Translation: DAA06753.1.
PIRiS46701.
RefSeqiNP_011927.1. NM_001179190.1. [P38784-1]

Genome annotation databases

EnsemblFungiiYHR060W; YHR060W; YHR060W. [P38784-1]
GeneIDi856457.
KEGGisce:YHR060W.

Keywords - Coding sequence diversityi

Alternative initiation

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U24501 Genomic DNA. Translation: AAA87458.1.
U00061 Genomic DNA. Translation: AAB68380.1.
BK006934 Genomic DNA. Translation: DAA06753.1.
PIRiS46701.
RefSeqiNP_011927.1. NM_001179190.1. [P38784-1]

3D structure databases

ProteinModelPortaliP38784.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi36492. 51 interactions.
DIPiDIP-1736N.
IntActiP38784. 9 interactions.
MINTiMINT-386505.

Proteomic databases

MaxQBiP38784.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYHR060W; YHR060W; YHR060W. [P38784-1]
GeneIDi856457.
KEGGisce:YHR060W.

Organism-specific databases

EuPathDBiFungiDB:YHR060W.
SGDiS000001102. VMA22.

Phylogenomic databases

HOGENOMiHOG000000873.
InParanoidiP38784.
OMAiRANYHNK.
OrthoDBiEOG72ZCSC.

Enzyme and pathway databases

BioCyciYEAST:G3O-31113-MONOMER.

Miscellaneous databases

NextBioi982096.
PROiP38784.

Family and domain databases

ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Vma22p is a novel endoplasmic reticulum-associated protein required for assembly of the yeast vacuolar H(+)-ATPase complex."
    Hill K.J., Stevens T.H.
    J. Biol. Chem. 270:22329-22336(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  4. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  5. Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT ALA-2 (ISOFORM 2), CLEAVAGE OF INITIATOR METHIONINE [LARGE SCALE ANALYSIS] (ISOFORM 2), IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiVMA22_YEAST
AccessioniPrimary (citable) accession number: P38784
Secondary accession number(s): D3DL09
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: February 1, 1995
Last modified: May 11, 2016
This is version 115 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 752 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  2. Yeast chromosome VIII
    Yeast (Saccharomyces cerevisiae) chromosome VIII: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.