P38784 (VMA22_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Vacuolar ATPase assembly protein VMA22

Gene names

Name:	VMA22
Ordered Locus Names:	YHR060W

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	181 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Required for V-ATPase activity.
Miscellaneous	Present with 752 molecules/cell in log phase SD medium. Ref.4

Ontologies

Keywords
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	vacuolar acidification Inferred from mutant phenotype. Source: SGD vacuolar proton-transporting V-type ATPase complex assembly Inferred from mutant phenotype Ref.1. Source: SGD
Cellular component	extrinsic to endoplasmic reticulum membrane Inferred from direct assay. Source: SGD vacuole Inferred from mutant phenotype Ref.1. Source: GOC
Molecular function	unfolded protein binding Inferred from mutant phenotype Ref.1. Source: SGD
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
VMA6	P32366	4	EBI-20329,EBI-20201

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 181	181	Vacuolar ATPase assembly protein VMA22		PRO_0000065876

Sequences

	Sequence		Length	Mass (Da)
	P38784 [UniParc]. Last modified February 1, 1995. Version 1. Checksum: C1CB5DC8292D2734	FASTA	181	21,078
	10 20 30 40 50 60 MSETRMAQNM DTTDEQYLRL IELLSNYDST LEQLQKGFQD GYIQLSRSNY YNKDSLRGNY 70 80 90 100 110 120 GEDYWDETYI GQLMATVEEK NSKVVVEIVK RKAQDKQEKK EEEDNKLTQR KKGTKPEKQK 130 140 150 160 170 180 TQSHKLKQDY DPILMFGGVL SVPSSLRQSQ TSFKGCIPLI AQLINYKNEI LTLVETLSEQ E

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Vma22p is a novel endoplasmic reticulum-associated protein required for assembly of the yeast vacuolar H(+)-ATPase complex." Hill K.J., Stevens T.H. J. Biol. Chem. 270:22329-22336(1995) [PubMed: 7673216] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Complete nucleotide sequence of Saccharomyces cerevisiae chromosome VIII." Johnston M., Andrews S., Brinkman R., Cooper J., Ding H., Dover J., Du Z., Favello A., Fulton L., Gattung S., Geisel C., Kirsten J., Kucaba T., Hillier L.W., Jier M., Johnston L., Langston Y., Latreille P. , Louis E.J., Macri C., Mardis E., Menezes S., Mouser L., Nhan M., Rifkin L., Riles L., St Peter H., Trevaskis E., Vaughan K., Vignati D., Wilcox L., Wohldman P., Waterston R., Wilson R., Vaudin M. Science 265:2077-2082(1994) [PubMed: 8091229] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[3]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[4]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U24501 Genomic DNA. Translation: AAA87458.1. U00061 Genomic DNA. Translation: AAB68380.1. BK006934 Genomic DNA. Translation: DAA06753.1.
PIR	S46701.
RefSeq	NP_011927.1. NM_001179190.1.
3D structure databases
ProteinModelPortal	P38784.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-1736N.
IntAct	P38784. 10 interactions.
MINT	MINT-386505.
STRING	P38784.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YHR060W; YHR060W; YHR060W.
GeneID	856457.
KEGG	sce:YHR060W.
NMPDR	fig|4932.3.peg.3080.
Organism-specific databases
CYGD	YHR060w.
SGD	S000001102. VMA22.
Phylogenomic databases
eggNOG	fuNOG11740.
HOGENOM	HBG203755.
OMA	RANYHNK.
OrthoDB	EOG4HX89W.
Gene expression databases
ArrayExpress	P38784.
Genevestigator	P38784.
GermOnline	YHR060W. Saccharomyces cerevisiae.
Family and domain databases
ProtoNet	Search...
Other
NextBio	982096.

Entry information

Entry name

VMA22_YEAST

Accession

Primary (citable) accession number: P38784
Secondary accession number(s): D3DL09

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1995
Last sequence update:	February 1, 1995
Last modified:	December 14, 2011
This is version 75 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program