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Protein

Chromatin structure-remodeling complex protein RSC30

Gene

RSC30

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Component of the chromatin structure-remodeling complex (RSC), which is involved in transcription regulation and nucleosome positioning. RSC is responsible for the transfer of a histone octamer from a nucleosome core particle to naked DNA. The reaction requires ATP and involves an activated RSC-nucleosome intermediate. Remodeling reaction also involves DNA translocation, DNA twist and conformational change. As a reconfigurer of centromeric and flanking nucleosomes, RSC complex is required both for proper kinetochore function in chromosome segregation and, via a PKC1-dependent signaling pathway, for organization of the cellular cytoskeleton. This subunit is required for transcription of ribosomal protein genes and genes involved in the integrity of the cell wall. Together with HTL1, LDB7, NPL6, RSC3 components, defines a fungal-specific module within the RSC complex that plays a role in many cellular functions including the maintenance of cell wall integrity.8 Publications

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
DNA bindingi14 – 45Zn(2)-C6 fungal-typePROSITE-ProRule annotationAdd BLAST32

GO - Molecular functioni

GO - Biological processi

  • covalent chromatin modification Source: UniProtKB-KW
  • double-strand break repair via nonhomologous end joining Source: SGD
  • nucleosome disassembly Source: SGD
  • regulation of transcription, DNA-templated Source: SGD
  • transcription elongation from RNA polymerase II promoter Source: SGD

Keywordsi

Molecular functionChromatin regulator, DNA-binding
Biological processTranscription, Transcription regulation
LigandMetal-binding, Zinc

Enzyme and pathway databases

BioCyciYEAST:G3O-31110-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Chromatin structure-remodeling complex protein RSC30
Alternative name(s):
Remodel the structure of chromatin complex subunit 30
Gene namesi
Name:RSC30
Ordered Locus Names:YHR056C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome VIII

Organism-specific databases

EuPathDBiFungiDB:YHR056C.
SGDiS000001098. RSC30.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi15C → G: Complete inactivation. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001149741 – 883Chromatin structure-remodeling complex protein RSC30Add BLAST883

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei150PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP38781.
PRIDEiP38781.
TopDownProteomicsiP38781.

PTM databases

iPTMnetiP38781.

Interactioni

Subunit structurei

Forms a heteromer with RSC3. Interacts with NPL6. Component of the two forms of the RSC complex composed of at least either RSC1 or RSC2, and ARP7, ARP9, LDB7, NPL6, RSC3, RSC30, RSC4, RSC58, RSC6, RSC8, RSC9, SFH1, STH1, HTL1 and probably RTT102. The complexes interact with histone and histone variant components of centromeric chromatin. Component of a fungal-specific module (HTL1-LDB7-NPL6-RSC3-RSC30) within the RSC complex.3 Publications

Binary interactionsi

Show more details

Protein-protein interaction databases

BioGridi36488. 32 interactors.
DIPiDIP-4275N.
IntActiP38781. 34 interactors.
MINTiMINT-510758.
STRINGi4932.YHR056C.

Structurei

3D structure databases

ProteinModelPortaliP38781.
SMRiP38781.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Phylogenomic databases

GeneTreeiENSGT00390000004801.
KOiK11758.
OrthoDBiEOG092C0LSK.

Family and domain databases

CDDicd00067. GAL4. 1 hit.
Gene3Di4.10.240.10. 1 hit.
InterProiView protein in InterPro
IPR001138. Zn2-C6_fun-type_DNA-bd.
PfamiView protein in Pfam
PF00172. Zn_clus. 1 hit.
SMARTiView protein in SMART
SM00066. GAL4. 1 hit.
SUPFAMiSSF57701. SSF57701. 1 hit.
PROSITEiView protein in PROSITE
PS00463. ZN2_CY6_FUNGAL_1. 1 hit.
PS50048. ZN2_CY6_FUNGAL_2. 1 hit.

Sequencei

Sequence statusi: Complete.

P38781-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MMDMQVRKVR KPPACTQCRK RKIGCDRAKP ICGNCVKYNK PDCFYPDGPG
60 70 80 90 100
KMVAVPSASG MSTHGNGQGS NHFSQGNGVN QKNVMIQTQY PIMQTSIEAF
110 120 130 140 150
NFSFNPSVDT AMQWTKAASY QNNNTNNNTA PRQNSSTVSS NVHGNTIVRS
160 170 180 190 200
DSPDVPSMDQ IREYNTRLQL VNAQSFDYTD NPYSFNVGIN QDSAVFDLMT
210 220 230 240 250
SPFTQEEVLI KEIDFLKNKL LDLQSLQLKS LKEKSNLNAD NTTANKINKT
260 270 280 290 300
GENSKKGKVD GKRAGFDHQT SRTSQSSQKY FTALTITDVQ SLVQVKPLKD
310 320 330 340 350
TPNYLFTKNF IIFRDHYLFK FYNILHDICH INQFKVSPPN NKNHQQYMEV
360 370 380 390 400
CKVNFPPKAI IIETLNSESL NNLNIEEFLP IFDKTLLLEF VHNSFPNGDT
410 420 430 440 450
CPSFSTVDLP LSQLTKLGEL TVLLLLLNDS MTLFNKQAIN NHVSALMNNL
460 470 480 490 500
RLIRSQITLI NLEYYDQETI KFIAITKFYE SLYMHDDHKS SLDEDLSCLL
510 520 530 540 550
SFQIKDFKLF HFLKKMYYSR HSLLGQSSFM VPAAENLSPI PASIDTNDIP
560 570 580 590 600
LIANDLKLLE TQAKLINILQ GVPFYLPVNL TKIESLLETL TMGVSNTVDL
610 620 630 640 650
YFHDNEVRKE WKDTLNFINT IVYTNFFLFV QNESSLSMAV QHSSNNNKTS
660 670 680 690 700
NSERCAKDLM KIISNMHIFY SITFNFIFPI KSIKSFSSGN NRFHSNGKEF
710 720 730 740 750
LFANHFIEIL QNFIAITFAI FQRCEVILYD EFYKNLSNEE INVQLLLIHD
760 770 780 790 800
KILEILKKIE IIVSFLRDEM NSNGSFKSIK GFNKVLNLIK YMLRFSKKKQ
810 820 830 840 850
NFARNSDNNN VTDYSQSAKN KNVLLKFPVS ELNRIYLKFK EISDFLMERE
860 870 880
VVQRSIIIDK DLESDNLGIT TANFNDFYDA FYN
Length:883
Mass (Da):101,335
Last modified:November 1, 1995 - v2
Checksum:iE8542445950FAA93
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00061 Genomic DNA. Translation: AAB68385.2.
BK006934 Genomic DNA. Translation: DAA06749.1.
RefSeqiNP_011923.2. NM_001179186.2.

Genome annotation databases

EnsemblFungiiYHR056C; YHR056C; YHR056C.
GeneIDi856453.
KEGGisce:YHR056C.

Similar proteinsi

Entry informationi

Entry nameiRSC30_YEAST
AccessioniPrimary (citable) accession number: P38781
Secondary accession number(s): D3DL05
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: November 1, 1995
Last modified: September 27, 2017
This is version 155 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  2. Yeast chromosome VIII
    Yeast (Saccharomyces cerevisiae) chromosome VIII: entries and gene names