Reviewed, UniProtKB/Swiss-Prot P38779 (CIC1_YEAST)

Names and origin

Protein names

Recommended name:
    Proteasome-interacting protein CIC1
Alternative name(s):
    Core interacting component 1

Gene names

Name:	CIC1
Synonyms:	NSA3
Ordered Locus Names:	YHR052W

Organism

Saccharomyces cerevisiae (Baker's yeast) [Complete proteome]

Taxonomic identifier

4932 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	376 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	An adapter protein that specifically links the 26S proteasome to its substrate CDC4 which is one of the substrate recognition subunits of the SCF E3 ubiquitin ligase complex. Required for turnover of cell cycle regulatory proteins CDC4 and GRR1. Required for synthesis and nuclear export of 60S ribosomal subunits. Required for vegetative growth.
Subunit structure	Interacts with CDC4, PRE4, PRE6, RPT1 and SCL1 as part of the fully assembled 26S proteasome. Interacts with pre-ribosomal particles constituent NOP7.
Subcellular location	Nucleus › nucleolus.
Miscellaneous	Present with 45900 molecules/cell in log phase SD medium.

Ontologies

Keywords
Biological process	Ribosome biogenesis
Cellular component	Nucleus Proteasome
PTM	Phosphoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	protein catabolic process Ref.3 Inferred from direct assay. Source: SGD ribosomal large subunit biogenesis Inferred from physical interaction. Source: SGD translation Inferred from electronic annotation. Source: InterPro
Cellular component	cytosol Inferred from electronic annotation. Source: UniProtKB-KW nucleolus Ref.3 Inferred from direct assay. Source: SGD preribosome, large subunit precursor Inferred from direct assay. Source: SGD proteasome complex Ref.3 Inferred from direct assay. Source: SGD ribosome Inferred from electronic annotation. Source: InterPro
Molecular function	protein binding, bridging Ref.3 Inferred from mutant phenotype. Source: SGD structural constituent of ribosome Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
CDC4	P07834	1	EBI-24538,EBI-4434
PRE6	P40303	1	EBI-24538,EBI-13980

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 376	376	Proteasome-interacting protein CIC1		PRO_0000202895
Regions
	Region	310 – 376	67	Required for interaction with CDC4
Amino acid modifications
	Modified residue	5	1	Phosphoserine
	Modified residue	7	1	Phosphoserine
	Modified residue	10	1	Phosphoserine
	Modified residue	11	1	Phosphothreonine
	Modified residue	14	1	Phosphoserine
	Modified residue	15	1	Phosphothreonine
	Modified residue	357	1	Phosphoserine
	Modified residue	359	1	Phosphoserine
	Modified residue	366	1	Phosphoserine

Sequences

	Sequence		Length	Mass (Da)
	P38779-1 [UniParc]. Last modified February 1, 1995. Version 1. Checksum: 13A2AFA0B927CD98	FASTA	376	42,530
	10 20 30 40 50 60 MAKKSNSKKS TPVSTPSKEK KKVIEKKSST AIPRERVIKA VNELIKFTSK PQDENNEEGN 70 80 90 100 110 120 NGKKNLLEDD EEELKKDLQL IVVNNKSFTG TSKSFKLKLL NVKHSFYKPW KEASATAVKD 130 140 150 160 170 180 FKVLLILKDS DIKKVSEDDL FDQLDSEGIK VDEIICGKDL KTVYKAYEAR NAFISQFSLI 190 200 210 220 230 240 LADDSIVTSL PKLMGGKAYN KVETTPISIR THANKEFSLT TLTNNIKKVY MNQLPVKLPR 250 260 270 280 290 300 GTTLNVHLGN LEWLRPEEFV DNVELISEQL IKAYQIRSIF IKTNRSPVLP LYYNQDVLDE 310 320 330 340 350 360 LEAKKDKIEE THEDDMVTID GVQVHLSTFN KGLMEIANPS ELGSIFSKQI NNAKKRSSSE 370 LEKESSESEA VKKAKS

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Complete nucleotide sequence of Saccharomyces cerevisiae chromosome VIII." Johnston M., Andrews S., Brinkman R., Cooper J., Ding H., Dover J., Du Z., Favello A., Fulton L., Gattung S., Geisel C., Kirsten J., Kucaba T., Hillier L.W., Jier M., Johnston L., Langston Y., Latreille P. , Louis E.J., Macri C., Mardis E., Menezes S., Mouser L., Nhan M., Rifkin L., Riles L., St Peter H., Trevaskis E., Vaughan K., Vignati D., Wilcox L., Wohldman P., Waterston R., Wilson R., Vaudin M. Science 265:2077-2082(1994) [PubMed: 8091229] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[2]	"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae." Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J. Genome Res. 17:536-543(2007) [PubMed: 17322287] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[3]	"Cic1, an adaptor protein specifically linking the 26S proteasome to its substrate, the SCF component Cdc4." Jaeger S., Strayle J., Heinemeyer W., Wolf D.H. EMBO J. 20:4423-4431(2001) [PubMed: 11500370] [Abstract] Cited for: FUNCTION, INTERACTION WITH PROTEASOME SUBUNITS, SUBCELLULAR LOCATION.
[4]	"Composition and functional characterization of yeast 66S ribosome assembly intermediates." Harnpicharnchai P., Jakovljevic J., Horsey E., Miles T., Roman J., Rout M., Meagher D., Imai B., Guo Y., Brame C.J., Shabanowitz J., Hunt D.F., Woolford J.L. Jr. Mol. Cell 8:505-515(2001) [PubMed: 11583614] [Abstract] Cited for: INTERACTION WITH NOP7.
[5]	"Phosphoproteome analysis by mass spectrometry and its application to Saccharomyces cerevisiae." Ficarro S.B., McCleland M.L., Stukenberg P.T., Burke D.J., Ross M.M., Shabanowitz J., Hunt D.F., White F.M. Nat. Biotechnol. 20:301-305(2002) [PubMed: 11875433] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-7 AND THR-11, MASS SPECTROMETRY.
[6]	"Global analysis of protein localization in budding yeast." Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K. Nature 425:686-691(2003) [PubMed: 14562095] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[7]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[8]	"Cic1p/Nsa3p is required for synthesis and nuclear export of 60S ribosomal subunits." Fatica A., Oeffinger M., Tollervey D., Bozzoni I. RNA 9:1431-1436(2003) [PubMed: 14623999] [Abstract] Cited for: FUNCTION.
[9]	"Proteome-wide identification of in vivo targets of DNA damage checkpoint kinases." Smolka M.B., Albuquerque C.P., Chen S.H., Zhou H. Proc. Natl. Acad. Sci. U.S.A. 104:10364-10369(2007) [PubMed: 17563356] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-5; SER-7; SER-10; THR-11; SER-14; THR-15; SER-357; SER-359 AND SER-366, MASS SPECTROMETRY.
[10]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed: 18407956] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-10; THR-11; SER-359 AND SER-366, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	U00062 Genomic DNA. Translation: AAB68898.1. AY557834 Genomic DNA. Translation: AAS56160.1.
PIR	S46729.
RefSeq	NP_011919.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
DIP	DIP:6491N.
IntAct	P38779.
Proteomic databases
PeptideAtlas	P38779.
Genome annotation databases
Ensembl	YHR052W. Saccharomyces cerevisiae. [Contig view]
GeneID	856449.
GenomeReviews	Gene locus YHR052W in contig U00093_GR.
KEGG	sce:YHR052W.
NMPDR	fig|4932.3.peg.3069.
Organism-specific databases
CYGD	YHR052w.
SGD	S000001094. CIC1.
Yeast-GFP	Search...
Phylogenomic databases
HOGENOM	P38779.
Gene expression databases
GermOnline	YHR052W. Saccharomyces cerevisiae.
Family and domain databases
ProtoNet	Search...
Other Resources
LinkHub	P38779.
NextBio	982072.

Entry information

Entry name

CIC1_YEAST

Accession

Primary (citable) accession number: P38779

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1995
Last sequence update:	February 1, 1995
Last modified:	November 25, 2008
This is version 60 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)