Proteasome-interacting protein CIC1 - Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

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P38779 (CIC1_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified December 14, 2011. Version 91. History...

Clusters with 100%, 90%, 50% identity |

Documents (2) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Proteasome-interacting protein CIC1

Alternative name(s):
Core interacting component 1

Gene names

Name:	CIC1
Synonyms:	NSA3
Ordered Locus Names:	YHR052W

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	376 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	An adapter protein that specifically links the 26S proteasome to its substrate CDC4 which is one of the substrate recognition subunits of the SCF E3 ubiquitin ligase complex. Required for turnover of cell cycle regulatory proteins CDC4 and GRR1. Required for synthesis and nuclear export of 60S ribosomal subunits. Required for vegetative growth. Ref.4 Ref.9
Subunit structure	Interacts with CDC4, PRE4, PRE6, RPT1 and SCL1 as part of the fully assembled 26S proteasome. Interacts with pre-ribosomal particles constituent NOP7. Ref.4 Ref.5
Subcellular location	Nucleus › nucleolus Ref.4 Ref.7.
Miscellaneous	Present with 45900 molecules/cell in log phase SD medium. Ref.8

Ontologies

Keywords
Biological process	Ribosome biogenesis
Cellular component	Nucleus Proteasome
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	protein catabolic process Inferred from mutant phenotype Ref.4. Source: SGD ribosomal large subunit biogenesis Inferred from physical interaction. Source: SGD translation Inferred from electronic annotation. Source: InterPro
Cellular component	large ribosomal subunit Inferred from electronic annotation. Source: InterPro nucleolus Inferred from direct assay Ref.4. Source: SGD preribosome, large subunit precursor Inferred from direct assay Ref.5. Source: SGD proteasome complex Inferred from physical interaction. Source: SGD
Molecular function	RNA binding Inferred from electronic annotation. Source: InterPro identical protein binding Inferred from physical interaction Ref.4. Source: IntAct protein binding, bridging Inferred from mutant phenotype Ref.4. Source: SGD structural constituent of ribosome Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct
itself		2	EBI-24538,EBI-24538
CDC4	P07834	2	EBI-24538,EBI-4434
NOP7	P53261	4	EBI-24538,EBI-13145
PRE6	P40303	5	EBI-24538,EBI-13980

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 376

376

Proteasome-interacting protein CIC1

PRO_0000202895

Regions

Region

310 – 376

Required for interaction with CDC4

Amino acid modifications

Modified residue

Phosphoserine Ref.10

Modified residue

Phosphoserine Ref.6 Ref.10

Modified residue

Phosphoserine Ref.10 Ref.11

Modified residue

Phosphothreonine Ref.6 Ref.10 Ref.11

Modified residue

Phosphoserine Ref.10

Modified residue

Phosphothreonine Ref.10

Modified residue

357

Phosphoserine Ref.10

Modified residue

359

Phosphoserine Ref.10 Ref.11

Modified residue

366

Phosphoserine Ref.10 Ref.11

Sequences

Sequence

Length

Mass (Da)

Tools

P38779 [UniParc].

Last modified February 1, 1995. Version 1.
Checksum: 13A2AFA0B927CD98
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FASTA

376

42,530

        10         20         30         40         50         60 
MAKKSNSKKS TPVSTPSKEK KKVIEKKSST AIPRERVIKA VNELIKFTSK PQDENNEEGN 

        70         80         90        100        110        120 
NGKKNLLEDD EEELKKDLQL IVVNNKSFTG TSKSFKLKLL NVKHSFYKPW KEASATAVKD 

       130        140        150        160        170        180 
FKVLLILKDS DIKKVSEDDL FDQLDSEGIK VDEIICGKDL KTVYKAYEAR NAFISQFSLI 

       190        200        210        220        230        240 
LADDSIVTSL PKLMGGKAYN KVETTPISIR THANKEFSLT TLTNNIKKVY MNQLPVKLPR 

       250        260        270        280        290        300 
GTTLNVHLGN LEWLRPEEFV DNVELISEQL IKAYQIRSIF IKTNRSPVLP LYYNQDVLDE 

       310        320        330        340        350        360 
LEAKKDKIEE THEDDMVTID GVQVHLSTFN KGLMEIANPS ELGSIFSKQI NNAKKRSSSE 

       370 
LEKESSESEA VKKAKS

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Complete nucleotide sequence of Saccharomyces cerevisiae chromosome VIII." Johnston M., Andrews S., Brinkman R., Cooper J., Ding H., Dover J., Du Z., Favello A., Fulton L., Gattung S., Geisel C., Kirsten J., Kucaba T., Hillier L.W., Jier M., Johnston L., Langston Y., Latreille P. Vaudin M. Science 265:2077-2082(1994) [PubMed: 8091229] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[2]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[3]	"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae." Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. LaBaer J. Genome Res. 17:536-543(2007) [PubMed: 17322287] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[4]	"Cic1, an adaptor protein specifically linking the 26S proteasome to its substrate, the SCF component Cdc4." Jaeger S., Strayle J., Heinemeyer W., Wolf D.H. EMBO J. 20:4423-4431(2001) [PubMed: 11500370] [Abstract] Cited for: FUNCTION, INTERACTION WITH PROTEASOME SUBUNITS, SUBCELLULAR LOCATION.
[5]	"Composition and functional characterization of yeast 66S ribosome assembly intermediates." Harnpicharnchai P., Jakovljevic J., Horsey E., Miles T., Roman J., Rout M., Meagher D., Imai B., Guo Y., Brame C.J., Shabanowitz J., Hunt D.F., Woolford J.L. Jr. Mol. Cell 8:505-515(2001) [PubMed: 11583614] [Abstract] Cited for: INTERACTION WITH NOP7.
[6]	"Phosphoproteome analysis by mass spectrometry and its application to Saccharomyces cerevisiae." Ficarro S.B., McCleland M.L., Stukenberg P.T., Burke D.J., Ross M.M., Shabanowitz J., Hunt D.F., White F.M. Nat. Biotechnol. 20:301-305(2002) [PubMed: 11875433] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-7 AND THR-11, MASS SPECTROMETRY. Strain: 2124.
[7]	"Global analysis of protein localization in budding yeast." Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K. Nature 425:686-691(2003) [PubMed: 14562095] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[8]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[9]	"Cic1p/Nsa3p is required for synthesis and nuclear export of 60S ribosomal subunits." Fatica A., Oeffinger M., Tollervey D., Bozzoni I. RNA 9:1431-1436(2003) [PubMed: 14623999] [Abstract] Cited for: FUNCTION.
[10]	"Proteome-wide identification of in vivo targets of DNA damage checkpoint kinases." Smolka M.B., Albuquerque C.P., Chen S.H., Zhou H. Proc. Natl. Acad. Sci. U.S.A. 104:10364-10369(2007) [PubMed: 17563356] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-5; SER-7; SER-10; THR-11; SER-14; THR-15; SER-357; SER-359 AND SER-366, MASS SPECTROMETRY.
[11]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed: 18407956] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-10; THR-11; SER-359 AND SER-366, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U00062 Genomic DNA. Translation: AAB68898.1. AY557834 Genomic DNA. Translation: AAS56160.1. BK006934 Genomic DNA. Translation: DAA06745.1.
PIR	S46729.
RefSeq	NP_011919.1. NM_001179182.1.
3D structure databases
ProteinModelPortal	P38779.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-6491N.
IntAct	P38779. 102 interactions.
MINT	MINT-615309.
STRING	P38779.
Proteomic databases
PeptideAtlas	P38779.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YHR052W; YHR052W; YHR052W.
GeneID	856449.
KEGG	sce:YHR052W.
NMPDR	fig\|4932.3.peg.3069.
Organism-specific databases
CYGD	YHR052w.
SGD	S000001094. CIC1.
Phylogenomic databases
eggNOG	fuNOG04679.
GeneTree	EFGT00050000004202.
HOGENOM	HBG203749.
OrthoDB	EOG4Q2HQC.
Gene expression databases
ArrayExpress	P38779.
Genevestigator	P38779.
GermOnline	YHR052W. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR002143. Ribosomal_L1. IPR023674. Ribosomal_L1_SF. [Graphical view]
KO	K14783.
Pfam	PF00687. Ribosomal_L1. 1 hit. [Graphical view]
SUPFAM	SSF56808. Ribosomal_L1. 1 hit.
ProtoNet	Search...
Other
NextBio	982072.

Entry information

Entry name

CIC1_YEAST

Accession

Primary (citable) accession number: P38779
Secondary accession number(s): D3DL01

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1995
Last sequence update:	February 1, 1995
Last modified:	December 14, 2011
This is version 91 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program

Relevant documents

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

Yeast chromosome VIII

Yeast (Saccharomyces cerevisiae) chromosome VIII: entries and gene names

Names·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Binary interactions

With

Entry

#Exp.

IntAct

Notes

Sequence annotation (Features)

Molecule processing

Regions

Amino acid modifications

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents