Manganese transporter SMF2 - Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

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P38778 (SMF2_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified April 3, 2013. Version 102. History...

Clusters with 100%, 90%, 50% identity |

Documents (3) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Manganese transporter SMF2

Gene names

Name:	SMF2
Ordered Locus Names:	YHR050W

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces ›

Protein attributes

Sequence length	549 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	High-affinity manganese transporter involved in mobilizing manganese from vesicular stores iin conditions of low manganese ion concentrations. Ref.4 Ref.5
Subcellular location	Vacuole membrane; Multi-pass membrane protein. Note: Targeted to the vacuolar lumen in presence of excess manganese, where it is degraded. Ref.5
Sequence similarities	Belongs to the NRAMP family.

Ontologies

Keywords
Biological process	Transport
Cellular component	Membrane Vacuole
Domain	Transmembrane Transmembrane helix
Ligand	Manganese Metal-binding
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	cellular cobalt ion homeostasis Inferred from genetic interaction PubMed 9115231. Source: SGD cellular manganese ion homeostasis Inferred from mutant phenotype PubMed 11602606. Source: SGD cobalt ion transport Inferred from genetic interaction PubMed 9115231. Source: SGD manganese ion transport Inferred from mutant phenotype PubMed 11602606. Source: SGD
Cellular_component	cytoplasmic membrane-bounded vesicle Inferred from direct assay Ref.5. Source: SGD integral to membrane Inferred from sequence model PubMed 12192589. Source: SGD mitochondrion Inferred from direct assay PubMed 14576278 PubMed 16823961. Source: SGD vacuolar membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	inorganic cation transmembrane transporter activity Inferred from direct assay PubMed 10574989. Source: SGD metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 549

549

Manganese transporter SMF2

PRO_0000212606

Regions

Transmembrane

91 – 109

Helical; Potential

Transmembrane

130 – 147

Helical; Potential

Transmembrane

161 – 185

Helical; Potential

Transmembrane

196 – 214

Helical; Potential

Transmembrane

312 – 332

Helical; Potential

Transmembrane

350 – 372

Helical; Potential

Transmembrane

432 – 452

Helical; Potential

Transmembrane

521 – 541

Helical; Potential

Sequences

Sequence

Length

Mass (Da)

Tools

P38778 [UniParc].

Last modified February 1, 1995. Version 1.
Checksum: 4C200057B82D65E3
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FASTA

549

59,768

        10         20         30         40         50         60 
MTSQEYEPIQ WSDESQTNND SVNDAYADVN TTHESRRRTT LQPNSTSQSM IGTLRKYARF 

        70         80         90        100        110        120 
IGPGLMVSVS YMDPGNYSTA VAAGSAHRYK LLFSVLVSNF MAAFWQYLCA RLGAVTGLDL 

       130        140        150        160        170        180 
AQNCKKHLPF GLNITLYILA EMAIIATDLA EVVGTAISLN ILFHIPLALG VILTVVDVLI 

       190        200        210        220        230        240 
VLLAYKPNGS MKGIRIFEAF VSLLVVLTVV CFTVELFYAK LGPAKEIFSG FLPSKAVFEG 

       250        260        270        280        290        300 
DGLYLSLAIL GATVMPHSLY LGSGVVQPRL REYDIKNGHY LPDANDMDNN HDNYRPSYEA 

       310        320        330        340        350        360 
ISETLHFTIT ELLISLFTVA LFVNCAILIV SGATLYGSTQ NAEEADLFSI YNLLCSTLSK 

       370        380        390        400        410        420 
GAGTVFVLAL LFSGQSAGIV CTLSGQMVSE GFLNWTVSPA LRRSATRAVA ITPCLILVLV 

       430        440        450        460        470        480 
AGRSGLSGAL NASQVVLSLL LPFVSAPLLY FTSSKKIMRV QLNRTKELSR TTDKKPVADR 

       490        500        510        520        530        540 
TEDDETIELE EMGIGSSSQE RSLVSPAPEY KDMSNGMIVT VLAIIVWLII SGLNFYMLLG 


FTTGKEVHL

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Two related genes encoding extremely hydrophobic proteins suppress a lethal mutation in the yeast mitochondrial processing enhancing protein." West A.H., Clark D.J., Martin J., Neupert W., Hartl F.-U., Horwich A.L. J. Biol. Chem. 267:24625-24633(1992) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Complete nucleotide sequence of Saccharomyces cerevisiae chromosome VIII." Johnston M., Andrews S., Brinkman R., Cooper J., Ding H., Dover J., Du Z., Favello A., Fulton L., Gattung S., Geisel C., Kirsten J., Kucaba T., Hillier L.W., Jier M., Johnston L., Langston Y., Latreille P. Vaudin M. Science 265:2077-2082(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[3]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[4]	"The family of SMF metal ion transporters in yeast cells." Cohen A., Nelson H., Nelson N. J. Biol. Chem. 275:33388-33394(2000) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
[5]	"Saccharomyces cerevisiae expresses three functionally distinct homologues of the nramp family of metal transporters." Portnoy M.E., Liu X.F., Culotta V.C. Mol. Cell. Biol. 20:7893-7902(2000) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U00062 Genomic DNA. Translation: AAB68900.1. BK006934 Genomic DNA. Translation: DAA06742.1.
PIR	B45154.
RefSeq	NP_011917.1. NM_001179180.1.
3D structure databases
ProteinModelPortal	P38778.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-4171N.
MINT	MINT-497357.
STRING	4932.YHR050W.
Protein family/group databases
TCDB	2.A.55.1.2. metal ion (Mn2+-iron) transporter (Nramp) family.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YHR050W; YHR050W; YHR050W.
GeneID	856447.
KEGG	sce:YHR050W.
Organism-specific databases
CYGD	YHR050w.
SGD	S000001092. SMF2.
Phylogenomic databases
eggNOG	COG1914.
GeneTree	ENSGT00390000006526.
HOGENOM	HOG000152204.
KO	K12346.
OMA	ANAIALF.
OrthoDB	EOG42FWS3.
Gene expression databases
Genevestigator	P38778.
GermOnline	YHR050W. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR001046. Nat-R-assoc-macro_Nramp. [Graphical view]
PANTHER	PTHR11706. PTHR11706. 1 hit.
Pfam	PF01566. Nramp. 1 hit. [Graphical view]
PRINTS	PR00447. NATRESASSCMP.
TIGRFAMs	TIGR01197. nramp. 1 hit.
ProtoNet	Search...
Other
NextBio	982066.

Entry information

Entry name

SMF2_YEAST

Accession

Primary (citable) accession number: P38778
Secondary accession number(s): D3DKZ8

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1995
Last sequence update:	February 1, 1995
Last modified:	April 3, 2013
This is version 102 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program

Relevant documents

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

Yeast chromosome VIII

Yeast (Saccharomyces cerevisiae) chromosome VIII: entries and gene names

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Protein family/group databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents